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Historically, physiologists have believed steroid hormones act exclusively within the individual producing them. However, studies in mice have shown that bioactive 17β-estradiol (E2) is excreted in male urine and absorbed by female conspecifics where it binds to estrogen receptors in reproductive other tissues. This can lead to pregnancy disruption and/or cause precocious puberty in female conspecifics. In bats the transfer of tritium-labelled estradiol (3H-E2) from male to females has been shown during the mating season. I investigated the influence of season on 3H-E2 transfer and showed that females housed with 3H-E2 injected males had significantly higher levels of radioactivity in reproductive, neural, and peripheral tissues during reproductively relevant timepoints compared to the non-reproductive season. Because urine has been hypothesized as the vector of steroid hormone transmission in mice, I examined the natural patterns of sex steroids in the urine of male and female big brown bats (Eptesicus fuscus) across the annual reproductive cycle. I found that creatinine-adjusted E2, which corrects for animal hydration and activity, was significantly higher in male than female urine, and in adult compared to yearling urine. Seasonal differences in urinary E2 levels were observed within and between sexes. Finally, I designed a protocol to investigate the attractant properties of body odour and urine between bat sexes during the mating season. Using a two-alternative Y-maze arena, I found that female bats first approach the test arm containing urine of a male conspecific before exploring the arm with female urine. Females also tended to spend more time in the test-arm containing male urine and being in the male test-arm at the conclusion of the 5 min trial. My data supports the hypothesis that E2 has the potential to act as reproductive pheromone with urine as a likely vector. Given the close proximity of individual bats within a maternity roost, steroid hormone transfer between conspecifics quite likely occurs in nature and could have profound influences on female reproductive behaviour (e.g. receptivity) and physiology (e.g. estrus cycling). My research provides new evidence for the potential pheromonal actions of E2 in bats that is consistent with how sex steroids act as pheromones in other mammals. / Dissertation / Doctor of Philosophy (PhD) / Historically, physiologists have believed steroid hormones act exclusively within the individual producing them. However, studies in mice have shown that bioactive 17β-estradiol (E2) is excreted in male urine and absorbed by female conspecifics where it binds to estrogen receptors in reproductive and other tissues. This exogenous E2 can result in changes to female reproductive physiology and behaviour. Our lab has previously observed E2 to transfer between male and female captive big brown bats during the mating season. Research from this thesis provides evidence that the transfer of E2 from male to female bats is highest during times of mating and ovulation/fertilization. I also demonstrate that E2 naturally occurs in the urine of both male and females with age, sex, and seasonal differences in concentration. Lastly, I show that female bats are attracted to the urine of males. My research provides new evidence for the potential pheromonal actions of E2 in bats that is consistent with how sex steroids act as pheromones in other mammals.

Background: The parents’ child with type 1 diabetes are responsible to make glucose measurements several times a day and by that have the primary responsibility for the child's health. This results in a new life for the parents. Aim: To describe parents' experiences to live and care for a child with type 1 diabetes. Method: A qualitative literature-based method by systematically searching through Pubmed and Cinahl about 10 selected articles. Results: The article revealed five main themes: Parents' increased responsibility, Parents' longing for a normal life, How the surroundings lack of knowledge affects you, The Healthcare system responsibility, Parents' painful emotions and feelings. Based on these five themes eleven sub themes emerged: The fight for a stable blood sugar level, Constant surveillance, Not limits the child’s daily activities, Parents' life change, School staff and friends' significant support, Constantly educating the surroundings, The importance of Care support, Your own need for knowledge, Continuous worries and fear, Feeling guilt over right treatment and care of the child, Inevitable exhaustion and burnout. Discussion: A nurse can learn a lot from the parents' experiences of living with a child diagnosed with type 1 diabetes and by that create valuable support for the parents. The discussion involves parents' worries and feelings of fear and guilt. The very important responsibility that the health care system has is discussed in relation to the parents. Mothers were mainly the ones who distributed the care of their child’s type 1 diabetes

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