Thesis (MPhil)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: A thesis undertaken to quantify genotype-by-environment interaction within Eucalyptus grandis clones growing in the eastern portion of South Africa. Thirty one sites were selected to represent the "traditional" E. grandis growing areas of South Africa. Eleven common macro- site variables and twelve common micro- site soil variables were recorded at each site. Twenty seven E. grandis clones and four E. grandis hybrid clones were then evaluated over these 31 sites. An incomplete latin square design was used to evaluate the 31 test clones, and five E. grandis controls were incorporated into the trial design to link the 31 sites. Volume production, stem form, stem defects and survival were assessed at two and five years, as well as the disease infestation of three stem cankers at five years. The analytical methods which were used to evaluate and quantify the GEl portion of the study are the analysis of variance (ANOV A), correlation analysis, and joint regression analysis (IRA) together with the analysis of co-variance (ACOV AR). The growth-site association for volume production, stem form and Endothia disease infestation were investigated using factor analysis (FA), and equations derived for the species and for the individual clones using a stepwise multiple regression approach. GEl, as evaluated through JRA, revealed that an increase in site productivity lead to a positive linear response in productivity on a clonal level, and that there was a diverging or fanning pattern among the regression lines of the clones. This tendency was also observed for both the stem form and the Endothia infestation. Hence, no significant changes in the rankings of the clones were found, and only relevant differences between the clones were found to change significantly. Juvenile-mature genetic correlations for volume production and the stem form showed moderate (rg = 0,66 and rg = 0,70) correlations between the two and the five year assessments. On a species level, rainfall was the main environmental factor responsible for volume production, while latitude was the main influence on stem form and Endothia infestation. On an individual clone basis, some micro-site soil factor interaction within the clones was found for the growth-site response models. Keywords: Eucalyptus grandis, genotype environment interaction, clones, site factors, growth-site response, ANOV A, ACOV AR, GEl, FA, JRA, / AFRIKAANSE OPSOMMING: 'n Studie is ondemeem om die genotipe-omgewingsinteraksie van Eucalyptus grandis klone, wat in die oostelike deel van Suid-Afrika groei, te kwantifiseer. Eenen- dertig groeiplekke is geselekteer om die "tradisionele" E. grandis groeiplekke in Suid-Afrika te verteenwoordig. Elf gemeenskaplike makro-groeiplek veranderlikes en twaalf gemeenskaplike mikro-groeiplek veranderlikes is by elk van die groei areas opgeteken. Sewe-en-twintig E. grandis klone en vier E. grandis basterklone is daama oor hierdie 31 groeiplekke geevalueer. 'n Onvolledige Latynse roosterontwerp is gebruik om die 31 toetsklone te evalueer en vyf kontroles is gebruik om die groeiplekke gemeenskaplik te verbind. Volume produksie, stamvorm, stamdefekte en oorlewing is op twee- en vyfjarige ouderdomme geevalueer terwyl besmetting met drie stamkankers op vyf jaar beoordeel is. Die analitiese metodes wat gebruik was om genotipeomgewingsinteraksie te evalueer en te kwantifiseer is die variansie analise (ANOYA), korrelasie analise, en gesamentlike regressie analise (JRA) tesame met ko-variansie analise (ACOY AR). Die groeiplek assosiasie vir volume produksie, stamvorm en Endothia besmetting is ondersoek deur gebruik te maak van faktor analise (FA), en vergelykings is verkry vir die spesies en individuele klone deur gebruik van 'n stapsgewyse meervoudige regressie benadering. Genotipe-omgewingsinteraksie, soos geevalueer deur JRA, wys dat 'n toename in groeiplek produktiwiteit lei tot 'n positiewe lineere reaksie in produktiwiteit op klonale vlak en dat daar 'n divergerende patroon tussen die regressielyne van die klone is. Hierdie tendens is ook vir beide die stamvorm en Endothia besmetting waargeneem. Gevolglik is nie-beduidende veranderings in die rangorde van die klone gevind en slegs reletiewe verskille tussen klone is gevind. Onvolwasse-volwasse genetiese korrelasies vir volume produksie en stamvorm toon matige korrelasies (rg =0.66 en rg =0.70) tussen die twee- en vyfjaar metings. Op 'n spesiesvlak was reenval die oorheersende omgewingsfaktor verantwoordelik vir volume produksie terwyl die breedtegraad ligging stamvorm en Endothia besmetting bemvloed het. Op individuele kloonvlak het sommige mikro-groeiplek interaksie binne klone bygedra tot die groei en groeiplek reaksie modelle. Sleutelwoorde: Eucalyptus grandis, Genotipe-omgewingsinteraksie, klone, groeiplek faktore, groeiplek reaksie, ANOY A, ACOY AR, FA, JRA

Thesis (PhD (For))--Stellenbosch University, 2012. / ENGLISH ABSTRACT: A primary reason for the existence of the forest industry is to provide a renewable and natural resource for much needed timber and fibre products. Substantial improvements in management practices are required to increase forest volume and pulp yields for increased demand. Eucalyptus grandis clonal trees of age 6.75 years, grown in a Nelder 1a spacing experiment, were sampled and analysed to describe the effect of planting density on i) growth and yield, ii) wood properties and iii) pulp and paper quality. The main objective was to populate a fibre productivity index (FPI) which would be suitable from technical and economical perspectives. A material study was conducted on the wood and in addition, two methods were developed to further describe the variability of the forest resource to i) separate growth rings by means of wood density peaks from gamma-ray densitometry and ii) calibrate near infrared (NIR) prediction models. The results indicated that planting density did not influence the variability of wood density but mechanisms affecting available soil water are important. NIR prediction models were developed to rapidly and reliably assess wood properties on a non-destructive basis. The validation models for wood density, total pulp yield, kappa number and insoluble lignin returned high predictive ability. When applied to predict chemical properties from an independent data set, the outcomes were accurate in comparison with measured data. Growth and yield functions were developed for tree survival, dominant height and basal area. They accurately predicted outcomes as demonstrated by the goodness of fit and their logical behaviour tested over the range of planting densities. When the most extreme stand density treatments, 6809 and 275 trees per hectare (TPH) were evaluated for wood and fibre properties, the larger trees grown at 275 TPH, produced wood of better quality for pulp processing; basic wood density at 0.520 g cm–3 (21 % higher), fibre cell wall thickness at 2.10 μm (18.6 % thicker) and fibre lumen diameter at 8.16 μm (9.9 % lower) than for 6809 TPH. Intra-specific tree variability of wood and product properties increased from diameter at breast height (DBH) to 35 % and then decreased to 65 % of tree height. The effect of planting density was carried throughout the product value chain up to the paper manufacturing phase. Paper with higher bulk mass and thickness and more porous sheets is most likely to be made from lower planting densities (801 and 275 TPH), and stronger, smoother and denser paper is most likely to be made with trees at high planting densities (6809 or 2336 TPH). From the growth and yield and materials investigation, technical indicators identified to populate a fibre productivity index were: i) mean annual increment (MAI) as a forestry growth indicator, ii) wood density, summarising the composition of wood and, iii) pulp yield, the indicator of the amount of fibre processed through a chemical cooking process. Delivered cost of timber to the mill, was identified as the most suitable economic indicator which included fixed costs elements, variable costs and aspects of mill efficiency. The product of the technical and economic indicators concluded in a profit/loss scenario of producing 1 ton of pulp was deemed the best index to describe the entire and integrated value chain. This index, termed the Fibre Productivity Index (FPI) at the Mill, denoted as FPMill, is an integrated index that is easy to interpret in the realms of a forestry - pulp manufacturing, and can be used for differential pricing of timber for wood quality. / AFRIKAANSE OPSOMMING: 'n Primêre rede vir die bestaan van die bosbouindustrie is om ‘n hernubare, natuurlike hulpbron vir hout en vesel te voorsien. Aansienlike verbeterings in bestuurspraktyke is nodig om die houtvolume en pulpopbrengste vir die toename in aanvraag te verhoog. Eucalyptus grandis klonale bome met ‘n ouderdom van 6.75 jaar en wat in 'n Nelder 1a spasiëring eksperiment gegroei is, is versamel en ontleed om die effek van opstandsdigtheid te beskryf op a) groei en opbrengs, b) houteienskappe en c) pulp- en papiergehalte. Die hoofdoel was om 'n veselproduktiwiteitsindeks (FPI), wat geskik sou wees in terme van tegniese en ekonomiese perspektiewe, te ontwikkel. 'n Materiaalkundigestudie is op hout uitgevoer. Twee metodes is ontwikkel om die variasie in hout as natuurlike hulpbron te beskryf deur a) vroeëhout- en laathoutdigtheidspieke deur gammastraal-densitometrie van mekaar te skei en variasie in groeiringe te beskryf en b) daarstelling van naby-infrarooispektroskopiese (NIR) voorspellingsmodelle. Die resultate het aangedui dat aanplantingsdigtheid nie ‘n invloed het op die variasie van houtdigtheid nie, maar dat meganismes wat beskikbare grondwater bepaal, belangrik is. NIR-voorspellingsmodelle is ontwikkel om houteienskappe op 'n nie-destruktiewe manier betroubaar te kan evalueer. Die validasiemodelle vir houtdigtheid, pulpopbrengs, kappanommer en onoplosbare lignien, openbaar akkurate voorspellingsvermoë. Wanneer dit toegepas word om chemiese eienskappe van 'n onafhanklike datastel te voorspel, was die resultate akkuraat in vergelyking met gemete data. Groei- en opbrengsfunksies is ontwikkel vir mortaliteit, dominante boomhoogte en basale area. Akkurate voorspellingsuitkomste is verkry soos gedemonstreer deur die logiese gedrag wat getoets is vir alle plantdigthede. Toe die mees ekstreme opstansdigtheidbehandelings vir hul hout- en veseleienskappe geëvalueer is, was die hout van die groter bome, teen 275 stamme per hektaar (SPH), van beter gehalte. Dit was veral prominent vir houtdigtheid van 0.520 g cm-3 (21 % hoër), veselselwanddikte van 2.10 μm (18.6 % dikker) en vesellumendeursnit van 8.16 μm (9.9 % laer) as by die hoër (6809) SPH. Intra-spesifieke boomvariasie van hout- en produkeienskappe het toegeneem van deursnee op borshoogte (DBH) tot 35 % en dan weer afgeneem tot 65 % van die boomhoogte. Die effek van plantdigtheid is regdeur die produkwaardeketting tot by die papiervervaardigingstadium sigbaar. Papier met hoër basismassa en dikte, en meer poreuse papiervelle kan meer waarskynlik van laer aanplantdigtheid (801 en 275 TPH) bome gemaak kan word. Papier wat sterker, gladder en digter is, kan waarskynlik gemaak word van hout van bome teen hoë aanplantdigthede (6809 of 2336 SPH). Die veselproduktiwiteitindeks wat ontwikkel is uit die materiaalondersoek en tegniese aanwysers wat geïdentifiseer is sluit in i) gemiddelde jaarlikse aanwas, as 'n bosbou groei-indikator, ii) houtdigtheid, wat ‘n opsomming van die samestelling van hout is, en iii) pulpopbrengs; die aanduiding van die hoeveelheid vesel verwerk deur 'n chemiese verpulpingsproses. Gelewerde koste van hout by die pulpmeul is geïdentifiseer as die mees geskikte ekonomiese aanwyser wat vaste kosteelemente, veranderlike koste en aspekte van die meul se doeltreffendheid insluit. Die produk van die tegniese en ekonomiese aanwysers is saamgevat in 'n wins / verlies opsie vir die vervaardiging van 1 ton pulp, en is beskou as die mees geskikte indeks om die geïntegreerde waardeketting te beskryf. Dié indeks, die sogenaamde Vesel Produktiwiteitsindeks (VPI) by die Pulpmeul, aangedui as VPMeul, is 'n geïntegreerde indeks wat maklik is om te interpreteer in 'n bosbou - pulpvervaardigingsopset, en kan gebruik word in die differensiële prysbepaling van hout waarby die kwaliteit in ag geneem word.

Mais de 20 milhões de hectares em todo o mundo são atualmente destinados a plantações de Eucalyptus, sendo que o Brasil possui a segunda maior área. No ano de 2007 a rede internacional EUCAGEN, liderada pelo Brasil, África do Sul e Estados Unidos, surgiu com o objetivo de colaboração para a pesquisa genômica do eucalipto. A árvore escolhida para o sequenciamento (Brasuz) foi fornecida pelo Brasil e em 2011 as primeiras sequências foram disponibilizadas. Em todas as fases de seu desenvolvimento, o eucalipto está sob o constante ataque de patógenos, destacando-se a ferrugem, causada pelo Basideomiceto Puccinia psidii Winter como a mais importante doença em regiões tropicais. A doença vem se espalhando rapidamente pelo mundo e recentemente foi relatada na Austrália, centro de origem do eucalipto. Com o objetivo de estudar o mecanismo molecular da interação plantapatógeno entre Eucalyptus grandis e Puccinia psidii, estabeleceu-se um patossistema modelo composto por um isolado monopustular do fungo e plantas resistente e susceptível provenientes de uma progênie de meios irmãos da planta Brasuz. O desenvolvimento do patógeno nos genótipos selecionados foi analisado por meio de microscopia de luz e de epifluorescência, e permitiu o monitoramento da dinâmica do desenvolvimento do fungo nos dois genótipos, com a identificação de todas as etapas de desenvolvimento do patógeno no genótipo susceptível bem como o estágio em que o genótipo resistente bloqueia o seu desenvolvimento. Com base nesses resultados determinou-se seis intervalos de interesse para a realização da análise da expressão gênica por meio da técnica de RNA-Seq. As análises revelaram grandes diferenças no perfil transcricional dos dois genótipos em resposta à presença do patógeno, permitindo a identificação de genes conhecidamente envolvidos em mecanismos de defesa de plantas como Receptores LRR-Quinase, fatores de transcrição WRKY, MYBS e GRAS, Proteínas R TIR-NBS-LRR Proteína Induzida por Injúria e proteínas envolvidas em processos de degradação proteica como F-Box. A comparação dos resultados provenientes das análises histológicas e moleculares permitiram a elaboração de um modelo para explicar os principais processos envolvidos no mecanismo de resistência de Eucalyptus grandis à Puccinia psidii. / More than 20 million hectares are destined to Eucalyptus plantations worldwide and Brazil has the second largest planted area. The International Eucalyptus Genome Network, EUCAGEN, was created in 2007 in order to perform genomic research on Eucalyptus. Brazil provided the biological material from the model tree (Brasuz) to have the complete genome sequenced and the first sequences were released to the scientific community in 2011. During Eucalyptus development, it is constantly exposed to pathogen attack with one of the most threatening diseases being eucalyptus rust caused by the neotropical rust fungus Puccinia psidii Winter which is rapidly spreading around the world and was recently described in Australia. In order to try and understand the molecular plant-microbe interaction between E. grandis and P. psidii we have to create a model system by isolating the pathogen from a single pustle and select resistant and susceptible plants from half-sib population generated using Brasuz as the pollen receptor. We performed light and epifluorescence microscopy analyses, identified all of the stages of the fungal development and recognized the moment which the resistant genotype blocks pathogen development. Based on these results we selected six time points to carry out transcriptomic analysis. Using RNA-Seq analysis we were able to verify large differences in transcriptional profile between resistant and susceptible plants and identify genes known involved in plant defense response such as LRR recptor Kinase, transcription factors (WRKY, MYBS and GRAS), TIR-NBS-LRR Proteins, Woundinduced protein and proteins involved in protein degradation (F-Box). Comparing microscopy and transcriptomic results allowed us to propose a model to explain the molecular mechanism of resistance of Eucalyptus grandis to Puccinia psidii.

O lodo de esgoto (LE) aplicado em solos de florestas plantadas, com base no critério do nitrogênio, pode aumentar a produção de madeira sem causar danos ao ambiente florestal. Entretanto, há necessidade de pesquisas para avaliar o aproveitamento dos nutrientes contidos no LE e para recomendar um manejo adequado em relação a sua aplicação no solo complementada pela fertilização mineral, considerando-se os aspectos de produtividade e econômico. Assim, objetivou-se analisar o efeito das combinações de doses de LE e de fertilizante mineral nitrogenado e fosfatado sobre a produção de biomassa seca e de volume de madeira de Eucalyptus grandis após 95 meses do uso do lodo, e avaliar a viabilidade econômica da recomendação de LE e de sua complementação com adubo mineral nitrogenado e fosfatado para o produtor florestal. O experimento foi instalado em área comercial da Suzano, em Angatuba-SP, aplicando-se doses de lodo (0; 7,7; 15,4 e 23,1 Mg ha-1, base seca), e de fertilizante mineral nitrogenado (0; 46,9; 95,1 e 142 kg ha-1), fosfatado (0; 27,7; 55,9 e 84 kg ha-1, P2O5), e potássico (175 kg ha-1 de K2O para todos os tratamentos, devido ao baixo teor deste nutriente no lodo); em delineamento em blocos ao acaso, em esquema fatorial 4x4x4, totalizando 128 parcelas. Para o objetivo específico do presente estudo, efeito das doses de lodo, foram tomadas aleatoriamente combinações de doses de N e P e dentre dessas foram estudadas todas as doses de LE aplicadas, totalizando 52 parcelas. A produção de biomassa seca e de madeira, calculada pela equação de Smalian, foram determinadas a partir de medidas coletadas aos 95 meses após 8 anos do plantio do eucalipto. Os valores de receita e de custos foram levantados no ano de referência de 2013. Para a avaliação da viabilidade econômica, utilizou-se o método do valor presente líquido anualizado (VPLA), considerando-se taxas de juros de 6 e 12% ao ano e que o produtor florestal arcaria com 0 ou 100% do custo do transporte do LE. Houve efeito linear das doses de LE sobre a produção de biomassa seca de lenho, casca, folha e galho e de volume de madeira. O LE na dose de 23,1 Mg ha-1 de LE, equivalente a 150% da dose de LE recomendada pelo critério do nitrogênio, propiciou a maior produção de matéria seca total, 187 Mg ha-1, e de tronco, 173 Mg ha-1 e de volume, 529 m3 ha-1, com incremento médio anual de 66 m3 ha-1 ano-1. Ao considerar o critério para a aplicação de LE, com base em N, e que o produtor florestal arcou com todo o custo de transporte deste resíduo, observou-se que o tratamento com maior retorno econômico foi o que utilizou a dose de 15, 4 Mg ha-1 de LE e 84 kg ha-1 P2O5 a taxas de juros de 6% a.a.; e aquele que aplicou 7,7 Mg ha-1 de LE e 28 kg ha-1 P2O5 a taxas de juros de 12% a.a. Quando o produtor florestal foi isento do custo de transporte do LE, o tratamento mais viável economicamente foi o que usou doses de 15,4 Mg ha-1 de LE e 84 kg ha-1 P2O5 para taxas de juros de 6 e 12% a.a. / The sewage sludge (SS) applied in planted forest soils, based on the nitrogen criterion, may increase the production of wood without causing damage to the forest environment. However, there is a need for research to evaluate the use of nutrients contained in the SS and to recommend appropriate management regarding for its application on soil using mineral fertilizer supplements by aspects of productivity and economic. The objective were to analyze the effect of combinations of sewage sludge doses and nitrogen and phosphate mineral fertilizer on the production on dry matter content and wood production of Eucalyptus grandis at 95 months after planting, and to evaluate the economic viability of SS recommendation and its complementation with nitrogen and phosphate mineral fertilizer for the forest producer. The experiment was installed in a commercial area of Suzano, in Angatuba-SP, sludge doses are applying (0, 7.7, 15.4 and 23.1 Mg ha-1, dry base), and nitrogen mineral fertilizer (0; 46.9; 95.1 and 142 kg ha-1), phosphate (0, 27.7, 55.9 and 84 kg -1 P2O5), and potassium (175 kg ha-1 K2O for all treatments, due to the low content of this nutrient in SS); in a randomized block design in a factorial 4x4x4. For the specific purpose of this study, combinations were taken in doses of N and P and among all these SS doses were studied. The production of dry matter and wood, calculated by equation Smalian were determined from measurements collected at 95 months post-planting eucalyptus. The revenue and cost values were raised in the reference year 2013. For the evaluation of the economic viability, we used the method of annualized net present value (ANPV), considering 6 and 12% per year discount rates and that the forest producer would bear 0 or 100% of the cost of transport of sewage sludge. There was a linear effect of SS doses on the production of dry biomass of wood, bark, leaf and branches and wood volume. The dose of 23.1 Mg ha-1 SS, equivalent to 150% of SS dose recommended by the criterion nitrogen, provided the highest total dry matter, 187 Mg ha-1, and wood+bark of 173 Mg ha-1 and stem volume of 529 m3 ha-1, with average annual increment of 66 m3 ha-1 yr -1. When considering the criterion to the application of SS, based on N, and the forestry producer has borne all the shipping cost of this waste, it was observed that the highest economic return treatment was that using a dose of 15.4 Mg ha- 1 SS and 84 kg ha-1 P2O5 to 6% per year discount rate; and one that applied 7.7 Mg ha-1 SS and 28 kg ha-1 P2O5 to the interest discount rate to 12% per year rate. When the forest producer was exempted to pay the SS shipping cost, the most economically viable treatment was what used 15.4 Mg ha-1 doses of SS and 84 kg ha-1 P2O5 to 6 and 12% per year discount rates

O trabalho realizado constitui uma estratégia em Biotecnologia de Plantas para a investigação dos esquemas bioquímicos controladores da diferenciação celular no processo de enraizamento. Através da técnica da cultura de tecidos \"in vitro\" foram micropropagados clones de Eucalyptus grandis HILL ex MAIDEN (GO 250, GO 682 e GO 669) selecionadas no CEBTEC/USP. Em uma pesquisa preliminar foram estabelecidas e multiplicadas gemas, seguida de testes da composição de meios de cultura para elongação e enraizamento. Foram estudadas diversas possibilidades de balanceamento das substâncias reguladoras de crescimento - ácido indolilbutírico (AIB)/ benzilaminopurina (BAP). Os clones apresentaram comportamentos anatômico-fisiológicos (massa fresca e seca; teor protéico; taxas de multiplicação das brotações epicórmicas, elongação e enraizamento) diferenciados entre si. As frequências de enraizamento mostraram-se superiores às já observadas por outros autores. Foram estabelecidas, ainda, algumas alterações na composição dos meios de cultura empregados. Através de experimentos cinéticos foram determinados os padrões de indução e/ou inibição das enzimas fenilalanina amônia-liase (PAL) e glucose 6-fosfato-desidrogenase (G-6P-DH) importantes para a biossíntese de compostos flavonóides, considerados como os principais cofatores das auxinas endógenas no complexo iniciador do enraizamento. Verificou-se que embora o suprimento exógeno crescente de AIB tenha resultado em maiores taxas de enraizamento, as atividades da PAL e G-6P-DH não se mostraram significativamente alteradas. Contudo, foi observado também que as gemas que enraizaram apresentaram atividade da PAL significativamente superior, evidenciando maior fluxo do Metabolismo do Fenilpropano durante o enraizamento. / This study constitutes a strategy in Plant Biotechnology with the purpose to investigate the regulating biochemical development of the cellular differentiation in the rooting process. Through plant tissue culture \"in vitro\" technique, clones of Eucalyptus grandis Hill ex Maiden (GO 250, GO 682 e GO 669) selected at CEBTEC/USP, were micropropagated. In a preliminary study shoots were multiplied, followed by tests of media culture composition for shoot elongation and root initiation. Several possibilities and rates of plant growth regulators, indolilbutyric acid (IBA)/benzylaminopurine (BAP) were studied. The clones showed different anatomic-physiological behaviours (fresh and oven-dry weight; proteic content; epicormic shoots multiplication rates, elongation and rooting). The frequences of rooting were higher than those described by other authors. Some modifications in the composition of used cuIture media were also defined. Through kinetic experiments, induction and inhibition patterns of phenylalanine ammonia-lyase (PAL) and glucose 6- phosphate-dehydrogenase (G-6P-DH) important to the biossyntesis of flavonoid compounds were determined. They were considered as the main cofactors of endogenous auxins in the root stimulating complex. Although, the increasing exogenous supply of AIB resulted in higher rooting rates, the activities of PAl and G-6P-DH enzyme did not present alterations. However, it was also observed that shoots wich rooted, in the treatments, showed higher PAL activity. resulting in higher flux of phenylpropanoid metabolism during the rooting.

Os painéis à base de madeira vêm sendo amplamente utilizados em todo o mundo, em resposta à redução de oferta de madeira maciça em diversos segmentos da indústria madeireira, como em móveis, painéis, estruturas e outros componentes na construção civil. O grande volume de resíduos gerados pela agroindústria viabiliza o desenvolvimento de materiais alternativos e sustentáveis, destacando-se os painéis de partículas. Este trabalho apresenta um estudo da produção e avaliação de painéis de partículas de Eucalyptus grandis e casca de aveia, aderidas sob pressão com dois tipos de resinas (poliuretana à base de mamona e ureia formaldeído). O desempenho físico-mecânico dos painéis produzidos foi avaliado com base na ABNT NBR 14810:2006. Por meio de análise de variância (ANOVA), avaliou-se a influência dos fatores adotados: madeira de Eucalyptus grandis, com proporções mássicas de 70, 85 e 100%; casca de aveia, nas proporções de 15, 30 e 100%; e adesivos, nas proporções de 10, 12 e 14%, bem como a combinação entre ambos, em cada uma das variáveis respostas (propriedades físico-mecânicas) avaliadas. Os resultados apontaram excelentes propriedades físico-mecânicas, em alguns casos muito superiores aos requisitos estipulados pelas normas nacionais e internacionais. Desta maneira, ficou comprovado o bom desempenho dos painéis de partículas produzidos, além de sua compatibilidade para aplicações em indústrias, como de móveis, painéis, embalagens e na construção civil. / Wood-based panels have been widely used around the world, replacing solid wood in various segments of the industry, such as furniture, panels, structures and other building components. The large volume of waste generated by agro industry enables the development of alternative and sustainable materials, highlighting the particleboard. This paper presents a study about production and evaluation of particleboard of Eucalyptus grandis and oat hulls, bonded under pressure with two types of resins (polyurethane based on castor oil and urea formaldehyde). Physical-mechanical performance of the panels produced was evaluated based on ABNT NBR 14810:2006. A variance analysis (ANOVA) evaluated the factors influence: Eucalyptus grandis, with mass ratios of 70, 85 and 100%; oat hulls, in proportions of 15, 30 and 100%; adhesives, in proportions of 10, 12 and 14%, and the combination of both, in each of the response variables (physical and mechanical properties) evaluated. Results showed excellent physical-mechanical properties, in some cases much higher than national and international codes requirements. Thus, panel particles good performance was proved, in addition to its compatibility with applications in wood industries, such as furniture, panels, packaging and in building construction.

Thesis (MScFor (Forest and Wood Science))--University of Stellenbosch, 2007. / The study was undertaken to determine the potential physiological benefits to plants provided by the double colonisation of host plant roots by endomycorrhizal (AM) and ectomycorrhizal (ECM) fungi, when growing under normal and under salt stress conditions. Plants of the Eucalyptus grandis x E. camaldulensis clone were grown in a sterile soil with 0 and 75 mM NaCl and with or without infection with the fungi Glomus etunicatum (an AM fungus) and Pisolithus tinctorius (an ECM fungus). The Eucalyptus clone formed both ECM and AM in single and double inoculation. The mycorrhizal symbiosis did not provide any nutritional benefits to the hosts. The double colonisation had no effect on plant growth under normal growth conditions while single colonisations of AM and ECM reduced growth. Double colonisation reduced host plant specific leaf mass by 12% and increased total leaf area by 43% compared with the control under these growth conditions. This colonisation also reduced photosynthesis per leaf area by 29% compared with the control. The reduced photosynthesis of the double colonisation did not result in reduced plant growth because these plants may have had a high total plant photosynthesis because of their large total leaf area. The double symbiosis however did not reduce salt stress when host plants were exposed to 75 mM NaCl, while the AM fungus increased plant dry weight by 13% compared to the control. AM and ECM colonisation in the double colonised roots under salt stress was decreased by 18 and 43% compared to that in plants under normal growth. The reduced colonisation may have reduced the fungi’s abilities to be beneficial to the host plant. The double symbiosis is recommended based on the documented positive effects of this symbiosis to plant growth and the considered possible long-term benefits to host plants growing in saline soils.

This study investigated the physical and mechanical properties of particleboards formed of bamboo (Bambusa tuldoides), pine (Pinus taeda) and eucalyptus (Eucalyptus grandis) and their combinations. To this end, panels were made, defined by the use of each type of particle pure or mixed by two or three being set 0, 25, 50, 75 and 100% of dry weight proportion, totalling 36 panels. The physical tests performed were the moisture content, density, water absorption and thickness swelling and the mechanical were static bending, screw withdrawal and internal bond. In order to complement the study, non-destructive testing were performed by direct and indirect ultrasonic to obtain models relating destructive and nondestructive methods. The results were analysed by for simple correlation and regression. In general, homogeneous panels made of pine and eucalypt showed better results than pure bamboo. The inclusion of bamboo particles decreased, mainly, the mechanical properties of the panels. All particleboard were below the minimum standards in physical and mechanical tests, only internal bond in panels pure pinehad higher values than commercial standards. Treatments using a combination of all species were compared, graphically, and resulted in lower than homogeneous panels of bamboo, for water absorption after two and twenty four hours and swelling after two hours. However, showed superior performance in swelling in thickness after twenty four hours, modulus of rupture and modulus of elasticity in static bending, screw withdrawal and internal bond. The non-destructive direct method showed better correlations than the indirect one. The significant correlations of the direct dynamic modulus of elasticity, in order of significance, occurred with board density, screw withdrawal, and static modulus of rupture and modulus of elasticity. / Este estudo investigou as propriedades físicas e mecânicas dos painéis aglomerados constituídos de bambu (Bambusa tuldoides), pinus (Pinus taeda) e eucalipto (Eucalyptus grandis) e as combinações entre eles. Para tanto, foram confeccionados painéis, definidos pelo uso de cada espécie de partícula pura ou misturada a duas ou três sendo estabelecidos 0, 25, 50, 75 e 100% de proporção em relação a massa seca, totalizando 36 painéis. Os ensaios físicos realizados foram o teor de umidade, massa específica, absorção d água e o inchamento em espessura e os mecânicos foram a flexão estática, arrancamento de parafusos e ligação interna. Para complementar o estudo, foi realizado ensaio não destrutivo pelos métodos direto e indireto para se obter modelos entre os ensaios destrutivos e o não destrutivo. Os resultados obtidos foram submetidos ao teste de correlação simples e analisados por regressão. De modo geral, os painéis homogêneos de pinus e eucalipto apresentaram melhores resultados que dos painéis formados apenas por bambu. A inclusão de bambu prejudicou, principalmente, as propriedades mecânicas das chapas. Todos os painéis ficaram abaixo dos valores mínimos das normas para os ensaios físicos e mecânicos, apenas no ensaio de tração os painéis homogêneos de pinus obtiveram valores superiores aos padrões de comercialização. Os tratamentos utilizando a combinação de todas as espécies foram comparados, graficamente, e resultaram em painéis inferiores aos homogêneos de bambu, para a absorção d água após duas e vinte quatro horas e inchamento após duas horas. Porém, apresentaram desempenhos superiores no inchamento após vinte quatro horas, módulos de ruptura e elasticidade estáticos, arrancamento de parafusos e ligação interna. Para os ensaios não destrutivos o método de propagação direto apresentou melhores correlações quando comparado ao indireto. As correlações significativas do módulo de elasticidade dinâmico direto, em ordem de significância, ocorreram com a massa específica observada, o arrancamento de parafusos, módulo de ruptura e módulo de elasticidade estático.

Resumo: O presente trabalho teve por objetivo avaliar o emprego da reflectometria no domínio do tempo, conhecida também, como TDR (Time Domain Reflectometry) na obtenção da umidade em madeiras. Atualmente esta técnica é muito empregada na física dos solos e sistemas de irrigação, para o monitoramento do teor de água no solo. Para este estudo, empregou-se duas espécies de madeiras comerciais, o Pinus caribaea var. hondurensis e a Grevillea robusta (Cunn.), procedentes dos municípios de Cabrália Paulista-SP e Duartina-SP. Para cada espécie, foram escolhidas de forma aleatória 11 árvores onde estas foram abatidas, serradas em toras de 3,0m e encaminhadas para serraria onde foi realizado o processo de desdobramento em tábuas pelo sistema de corte tangencial. As tábuas apresentaram espessura de 28mm e largura de 24cm. Em seguida, escolheu-se novamente de forma aleatória 11 tábuas, que foram embaladas em plástico para evitar a perda de água, e encaminhadas para o laboratório. Uma vez obtidas as amostras representativas para avaliação, estas foram inicialmente pesadas e secas em estufa elétrica a 40 oC de temperatura. Para o Pinus caribaea var. hondurensis, o processo de secagem iniciou-se com umidade média de 101,45% e finalizou, à 7,36% de umidade. Já para a Grevillea robusta (Cunn.), a umidade média inicial foi de 125,88% e final de 6,62%. Durante o processo de secagem, a umidade foi determinada, periodicamente, através do método de massas correntes, bem como, a respectiva constante dielétrica relativa pela TDR. Para o estudo da viabilização da TDR na determinação... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The main objective of this present work was to evaluate the applied of the reflectometry in the domain of time, technique known as TDR (Time Domain Reflectometry) to obtention of moisture in woods. Nowadays, this technique is very used in soil physics and irrigation system, to the monitoring of moisture in the soil. To this study, two species of commercial woods were used, the Pinus caribaea var. hondurensis and the Grevillea robusta (Cunn.), these woods come from the municipal district of Cabrália Paulista-SP and Duartina-SP. To each specie, were chosen in a casual way, 11 trees. They were dejected, planked in logs of 3,0m and referred to the sawmill, the place where was realized the process of unfolding the wood in boards by the system of tangential section. The boards brung up the ply of 28mm and the width of 24cm. Next, were chosen again, in a casual way, 11 planks that were unrolled in plastic just to avoid the waterless, and referred to the lab. When were obtained the appropriate samples to the evaluation, they were, in an initial instant, measure out and dry in an electric greenhouse of a temperature of 40 oC. To Pinus caribaea var. hondurensis, the dry process started with a media moisture of 101,45% and finished with 7,36% of moisture. To the other specie, the Grevillea robusta (Cunn.), the initial media moisture found was 125,88% and the final was 6,62%. During the whole dry process, the moisture was determinated, periodically, throw the masses current, as like the relative dielectric constant by the TDR. To the study of the feasibility of TDR in the determination of moisture in woods, was chosen to establish 3 distances among the sensor's stick: 10mm, 20mm and 30mm (these distances were applied in commercial resistive gauges too). In the same way, it was established the variations in the velocity of propagation of electromagnetic pulses send out from TDR equipment... (Complete abstract click electronic access below) / Orientador: João Eduardo Guarnetti dos Santos / Coorientador: Antonio Evaldo Klar / Coorientador: Elias Taylor Durgante Severo / Banca: Marcos Antonio de Rezende / Banca: Momotaro Imaizumi / Banca: José Fernandes Presenço / Banca: Eik Teório / Doutor

The express wood volume in meter estere (mst) sometimes need to be converted into cubical meters (m3). This is possible estimating a cubication factor (Fc). With the objective to determine the Fc for Eucalyptus grandis and its variation in pieces of wood of different lengths of 1 and 2,3m of length in reason of the time of exposure to the enviroment, in the summer and the winter. Six stacks of wood pieces 3X2X1m (width, height and depth) dimensions had been prepared and six stacks of wood pieces in the dimensions 3X2X2,3m, installed in the summer and winter. The Fc was obtained by volume reason, with CV of 4,2 to 9,5% and by angular counting with CV of 10,9 to 17%. Thus, continued analysis with Fc s average obtained by reason of volumes because they had presented minor variation. The analysis statistics for factorial experiment(2X2X6), indicated significant difference (α<5%) in the variations of principal effect, that is, in the climatic station (F=18,72) , in the time of exposure (F=6,51) and in the length of the different pieces of wood (F=35,18) .The analysis of the correlation of Pearson indicated that the Fc was also influenced by the cracks on top, the humidity of the wood and the atmospheric variable, in the two observed seasons. Evidenced variation of the Fc of the stacks in exposition, equation with use of Stepewise procedure was shaped for the independent variable: T, T2, T3, T4, T5, 1/T, 1/T2, 1/T3, 1/T4, 1/T5, log(T), log(1/T), log(lnT), 1/lnT, 1/log(T), the equation with the best adjustment was decurrent of short pieces of wood of winter for an initial period until 90 days after installation. Adjusted the equation Fc=0,70385+0,00000961(T2), with values of F and R2 equal 17,22 (Pr>F=0,2%) e 0,63, respectively. However the averages of the value of the Fc obtained by observation and the estimator are the same (0,74) and the model was more accurate, then was opted to using the average gathered by each month. The conversion chart of the volume estere in cubical volume was completed since specific average values of the Fc for the climatic stations summer and winter and for the two lengths of pieces of wood, obtained by reason of volumes. The average values of the Fc s had indicated a stabilization after ninety days of the installation of the stacks, with this subdivided the time of exposure in two periods: one from zero to ninety days and another one from ninety to one hundred and eighty days, these compared with the total of the observations (180 days), the average values of these observations had not presented significant difference by the Tukey test (α<5%). However, pieces of wood of Eucalyptus grandis had presented dimensional variation when displayed in inclement weather for a period of one hundred and eighty days (six months) making the cubication factor Fc to vary in elapsing of the period of exposition. / O volume de madeira expresso em metro estere (mst) algumas vezes necessita ser convertido em metro cúbico (m3). Isso é possível estimando-se um fator de cubicação (Fc). Com o objetivo de determinar o Fc para Eucalyptus grandis e sua variação em toretes de 1 e de 2,3m de comprimento em razão do tempo de exposição ao ambiente, no verão e no inverno. Foram confeccionadas 6 pilhas de toretes nas dimensões 3x2x1m (largura, altura e profundidade) e 6 pilhas nas dimensões 3x2x2,3m, instaladas no verão e no inverno. O Fc foi obtido por razão de volume, com CV de 4,2 a 9,5% e por contagem angular com CV de 10,9 a 17%. Assim, prosseguiu análise com Fc s médios obtidos por razão de volumes pois apresentaram menor variação. A análise estatística para experimento fatorial (2x2x6), indicou diferença significativa (α<5%) nas variáveis do efeito principal, isto é, na estação climática (F=18,72), no tempo de exposição (F=6,51) e no comprimento do torete (F=35,18). A análise da correlação de Pearson indicou que o Fc também foi influenciado pelas rachaduras de topo, pela umidade da madeira e pelas variáveis atmosféricas, nas duas estações observadas. Constatada variação do Fc das pilhas em exposição, modelou-se equação com uso de procedimento Stepewise para as variáveis independentes: T, T2, T3, T4, T5, 1/T, 1/T2, 1/T3, 1/T4, 1/T5, log(T), log(1/T), log(lnT), 1/lnT, 1/log(T), a equação com o melhor ajuste foi a decorrente de toras curtas de inverno para um período inicial até 90 dias após a instalação. Ajustada a equação Fc=0,70385+0,00000961(T2), com valores de F e R2 iguais a 17,22 (Pr>F=0,2%) e 0,63, respectivamente. No entanto as médias do valor do Fc obtidas das observações e do estimador é a mesma (0,74) e o modelo foi tendencioso, optou-se por utilizar a média obtida a cada mês, a partir da exposição. A tabela de conversão do volume estere em volume cúbico foi confeccionada a partir dos valores médios do Fc específicos para as estações climáticas verão e inverno e para os dois comprimentos dos toretes, obtidos por razão de volumes. Os valores médios dos Fc s indicaram uma estabilização após noventa dias da instalação das pilhas, com isso subdividiu-se o tempo de exposição em dois períodos: um de zero a noventa dias e outro de noventa a cento e oitenta dias, esses comparados ao total das observações (180 dias) os valores médios dessas observações não apresentaram diferença significativa pelo teste Tukey (α<5%). No entanto, os toretes de Eucalyptus grandis apresentaram variação dimensional quando expostos as intempéries por um período de cento e oitenta dias (seis meses) fazendo o fator de cubicação (Fc) variar no decorrer do período de exposição.