Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-01-16T19:14:42Z No. of bitstreams: 1 VictorAraujoFerreiraMatos_DISSERT.pdf: 1450996 bytes, checksum: e18c336aa8ee5aeeb4bcf215b7398c7d (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-01-22T12:42:58Z (GMT) No. of bitstreams: 1 VictorAraujoFerreiraMatos_DISSERT.pdf: 1450996 bytes, checksum: e18c336aa8ee5aeeb4bcf215b7398c7d (MD5) / Made available in DSpace on 2018-01-22T12:42:58Z (GMT). No. of bitstreams: 1 VictorAraujoFerreiraMatos_DISSERT.pdf: 1450996 bytes, checksum: e18c336aa8ee5aeeb4bcf215b7398c7d (MD5) Previous issue date: 2017-07-10 / Introdu??o: O exerc?cio f?sico ? considerado uma estrat?gia fundamental no tratamento da obesidade por promover aumento no d?ficit cal?rico. Evid?ncias recentes sugerem que o exerc?cio de alta intensidade pode induzir altera??es fisiol?gicas que diminuem o apetite, contribuindo para redu??o da ingest?o cal?rica, fen?meno conhecido como anorexia induzida pelo exerc?cio (AIE). Objetivo: comparar o efeito agudo do exerc?cio intervalado de alta intensidade (EIAI) e exerc?cio cont?nuo de intensidade moderada (ECMI) sobre o consumo energ?tico e percep??o do apetite em homens com obesidade. M?todos: A pesquisa caracterizou-se como um ensaio cl?nico randomizado com delineamento cruzado. Quinze volunt?rios (31,0 ? 6,1 anos, 31,0 ? 6,0 kg/m?, 40,1 ? 2,2% de gordura corporal) foram submetidos a tr?s sess?es experimentais, separadas por sete dias: I) EIAI (10 x 1 min a 90% da frequ?ncia card?aca m?xima [FCm?x] + 1 min recupera??o ativa), II) ECMI (20 min a 70% da FCm?x) e III) Controle (sem exerc?cio). Uma refei??o padronizada foi oferecida 60 min antes de cada sess?o. A percep??o subjetiva do apetite (fome, saciedade, plenitude e perspectiva de consumo alimentar) foi avaliada por meio de uma escala visual anal?gica (EVA) em tr?s momentos: 1) pr?-sess?o, 2) p?s-sess?o e 3) uma hora ap?s sess?o. O consumo alimentar ad libitum foi analisado uma hora ap?s as sess?es experimentais e controle, por meio de registro pesado e o consumo alimentar ao longo do dia da sess?o (24 h) foi avaliado por meio de um registro alimentar estimado. A ANOVA two-way com medidas repetidas foi utilizada para analisar poss?veis diferen?as entre as condi??es e momentos na percep??o do apetite e uma ANOVA de medidas repetidas para analisar as diferen?as entre as condi??es no consumo alimentar ad libitum e 24 h. Resultados: N?o houve diferen?as significativas na percep??o de fome [F(1,3, 17,5)=0,00, p=0,972], saciedade [F(1,3, 17,5)=0,00, p=0,972], plenitude [F(2, 28)=0,13, p=0,876] e perspectiva de consumo [F(2, 28)=0,76, p=0,476] entre as condi??es, bem como n?o foram identificadas diferen?as significativas no consumo energ?tico na refei??o ad libitum: CON (674,5 ? 252,1), ECMI (666,7? 213,8) e EIAI (689,6? 263,8kcal) [F (2, 28)=0,13, p=0,877] e ao longo do dia da sess?o: CON (2857,6 ? 867,2), ECMI (2608,5? 595) e EIAI (2556,1? 489,5kcal) [F (2, 28) = 1,54, p=0,233]. Conclus?o: Uma sess?o de EIAI e ECMI n?o modificou o apetite e o consumo energ?tico ap?s 60 min e ao longo do dia em homens com obesidade. / Introduction: Physical exercise is considered a fundamental strategy in the treatment of obesity by promoting increase in the caloric deficit. Recent evidence suggests that high-intensity exercise can induce physiological changes that suppress appetite, decreasing caloric intake, a phenomenon known as exercise-induced anorexia (EIA). Objective: compare the acute effect of high intensity interval exercise (HIIE) and continuous moderate intensity exercise (ECM) on energy intake and appetite perception in obese men. Methods: The study was characterized as a randomized clinical trial in cross-over design. Fifteen volunteers (31.0 ? 6.1 years, 31.0 ? 6.0 kg/ m?, 40.1 ? 2.2% body fat) underwent three experimental sessions, seven days apart: I) HIIE ( 10x 1 min at 90% of maximal heart rate [HRmax] + 1 min active recovery), II) ECM (20min to 70% of HRmax) and III) Control (without exercise). A standardized meal was offered 60 min before each session. Appetite perception (hunger, satiety, fullness and perspective food consumption) were evaluated through a visual analogue scale (VAS) in three moments: 1) Pre-session, 2) Post-session and 3) one hour after session. Energy intake was assessed one hour after session with an ad libitum meal and the consumption throughout the day (24hrs) was analyzed with a estimated food record. ANOVA Two-way with repeated measures was used to analyze the differences between conditions and moments on appetite perception and ANOVA with repeated measures to assess the differences between conditions on energy intake in ad libitum and 24hrs. Results: There were no significant differences on hunger [F (1.3, 17.5) = 0.00, p = 0.972], satiety [F (1.3, 17.5) = 0.00, p = 0.972], fullness [F (2, 28) = 0.13, p = 0.876] and prospective food consumption [F (2, 28) = 0.76, p = 0.476] between conditions. As well as no significant differences on energy intake were observed during ad libitum meal: CON (674,5 ? 252,1), ECM (666,7? 213,8) e HIIE (689,6? 263,8kcal) [F (2, 28) = 0.13, p = 0.877] and throughout the day CON (2857,6 ? 867,2), ECM (2608,5? 595) e HIIE (2556,1? 489,5kcal) [F (2, 28) = 1.54, p = 0.233].Conclusion: A single session of HIIE and ECM did not modified appetite and energy intake 60 min after and during the day following exercise in obese man.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-06-23T21:52:18Z No. of bitstreams: 1 PedroIvoDeSouzaPinheiro_DISSERT.pdf: 1470768 bytes, checksum: 95e0a6d8271caf71927f8658f369b623 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-06-28T21:55:12Z (GMT) No. of bitstreams: 1 PedroIvoDeSouzaPinheiro_DISSERT.pdf: 1470768 bytes, checksum: 95e0a6d8271caf71927f8658f369b623 (MD5) / Made available in DSpace on 2016-06-28T21:55:12Z (GMT). No. of bitstreams: 1 PedroIvoDeSouzaPinheiro_DISSERT.pdf: 1470768 bytes, checksum: 95e0a6d8271caf71927f8658f369b623 (MD5) Previous issue date: 2015-05-27 / O exerc?cio intervalado de alta intensidade tem sido apontado como op??o para o aumento da pr?tica da atividade f?sica al?m de ser sugerido no manejo terap?utico de diversas condi??es como diabetes mellitus e insufici?ncia card?aca. Contudo, o conhecimento pleno das suas repercuss?es fisiol?gicas e dos par?metros que possam trazer maior seguran?a quanto ? sua prescri??o; em especial os efeitos a curto e m?dio prazo (24 horas ap?s o exerc?cio) sobre a recupera??o do exerc?cio, necessitam ser esclarecidos. O objetivo do presente trabalho ? avaliar a repercuss?o de uma sess?o de exerc?cio aer?bico cont?nuo e intervalado no controle auton?mico card?aco imediato e em m?dio prazo (24 horas), atrav?s da avalia??o da variabilidade da frequ?ncia card?aca (VFC). Trata-se de um ensaio cl?nico randomizado do tipo crossover onde indiv?duos jovens saud?veis e com baixo n?vel de atividade f?sica tiveram a VFC de 24 horas mensurada atrav?s de frequenc?metro e aceler?metro port?til (eMotion HRV 3D, Kuopio, Finl?ndia) antes e ap?s sess?o de exerc?cio aer?bio cont?nuo (60-70% FCmax, 21min.) e intervalado (ciclo 1 min. a 80-90% FCmax, 2 min. a 50-60% FCmax, dura??o 21 min.). A VFC foi avaliada nos dom?nio tempo e frequ?ncia e o balan?o simpatovagal determinado pela raz?o LF/HF. Avalia??o n?o linear foi calculada pela entropia de Shannon. O dados demonstraram retardo na recupera??o imediata da frequ?ncia card?aca p?s exerc?cio e menor FC 24 horas comparados a valores pr? interven??o, principalmente no exerc?cio intervalado. Houve tend?ncia ? maior predom?nio e valores de ?ndices representantes da estimula??o simp?tica durante o dia no grupo de exerc?cio intervalado; contudo, sem signific?ncia estat?stica. O resultados do estudo auxiliam no esclarecimento das repercuss?es do exerc?cio intervalado nas 24 horas que sucedem a interven??o permitindo par?metros para prescri??o e futura avalia??o de grupos de indiv?duos com patologias metab?licas e cardiovasculares. / The high-intensity interval exercise has been described as an option for increasing physical activity and its use also being suggested in the therapeutic management of many conditions such as diabetes mellitus and heart failure. However, the knowledge of its physiological effects and parameters that can assure greater safety for interval exercise prescription; especially its effect on short- and medium-term (24 hours after exercise) exercise recovery, need to be clarified. This study objective was to evaluate the effect of continuous and interval aerobic exercise on the cardiac autonomic control immediate and medium term (24 hours), by assessing heart rate variability (HRV). The present study is a randomized crossover clinical trial in which healthy young individuals with low level of physical activity had the VFC 24 hours measured by a heart rate sensor and portable accelerometer (3D eMotion HRV, Kuopio, Finland) before and after continuous aerobic exercise (60-70% HR max, 21 min.) and interval exercise (cycle 1 min. 80-90% HR max, 2 min. at 50-60% HR max, duration 21 min.). HRV was measured in the time and frequency domain and the sympathovagal balance determined by the ratio LF / HF. Nonlinear evaluation was calculated by Shannon entropy. The data demonstrated delayed heart rate recovery immediate after exercise and lower HR after 24 hours compared to pre intervention values, especially in the interval exercise group. There was a tendency to higher predominance and representatives index values of sympathetic stimulation during the day in interval exercise group; however, without statistical significance. The study results help to clarify the effects of interval exercise on the 24 hours following interval exercise, setting parameters for prescription and for further evaluation of groups with metabolic and cardiovascular diseases.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-04T17:06:18Z No. of bitstreams: 2 rosalina_tossige_gomes.pdf: 1360296 bytes, checksum: 20d9d22baa3b186a5cd4139e7af933da (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-04T17:06:52Z (GMT) No. of bitstreams: 2 rosalina_tossige_gomes.pdf: 1360296 bytes, checksum: 20d9d22baa3b186a5cd4139e7af933da (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-01-04T17:06:52Z (GMT). No. of bitstreams: 2 rosalina_tossige_gomes.pdf: 1360296 bytes, checksum: 20d9d22baa3b186a5cd4139e7af933da (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015 / O exerc?cio intervalado de alta intensidade (HIIE) ? caracterizado por breves e repetidas sess?es de exerc?cio intenso, intercaladas por per?odos de repouso ou exerc?cio de baixa intensidade. ? uma modalidade de exerc?cio que tem ganhado muito destaque nos ?ltimos anos por ser uma modalidade de treinamento de baixo volume, embora pouco se saiba a respeito dos seus efeitos na fun??o imune e no estado redox celular. Assim, este estudo avaliou o efeito de uma sess?o de HIIE sobre o estado redox e a fun??o de linf?citos em homens jovens sedent?rios. Esse trabalho foi dividido em dois estudos. No estudo 1 avaliou-se o efeito do HIIE sobre a prolifera??o e produ??o de citocinas e o estado redox de c?lulas mononucleares do sangue perif?rico (PBMC). No estudo 2 avaliou-se o efeito do HIIE sobre a viabilidade e express?o de marcadores de ativa??o em linf?citos. As sess?es de HIIE foram realizadas em bicicleta ergom?trica, e consistiram em oito s?ries de 1 min a 90-100% de pot?ncia pico, com 75 segundos de recupera??o ativa, a 30W, entre as s?ries. O sangue venoso foi colhido antes, imediatamente ap?s e 30 minutos ap?s a sess?o de HIIE. Para avalia??o da prolifera??o celular, por citometria de fluxo, as PBMC foram coradas com Carboxifluoresce?na Succinimidil Ester (CFSE) (10 ?M) e estimuladas com o superant?geno SEB (100 ng/mL), durante 5 dias a 37? C, 5% de CO2. A produ??o de IL-2 e IFN-? em resposta a estimula??o por SEB durante 18 horas, foi avaliada por ELISA. O estado redox celular foi avaliado pela mensura??o da atividade das enzimas catalase (CAT) e super?xido dismutase (SOD), a concentra??o de subst?ncias que reagem ao ?cido tiobarbit?rico (TBARS) e conte?do de glutationa reduzida (GSH). Para avalia??o da viabilidade celular, por citometria de fluxo, as c?lulas foram marcadas com anticorpos anti-Anexina V FITC e iodeto de prop?deo. Para an?lise da ativa??o dos linf?citos, por citometria de fluxo, as PBMC foram estimuladas com SEB por 18 horas, e em seguida marcadas com anticorpos fluorescentes dirigidos contra CD4, CD8, CD19, CD25 e CD69. Os dados foram analisados utitlizando os testes one-way ou two-way ANOVA, considerando p ? 0,05. O HIIE promoveu redu??o na prolifera??o de linf?citos (p = 0,01), aumento na concentra??o de IL-2 (p = 0,02), e desequil?brio redox nas PBMC, marcado por aumento nas concentra??es de TBARS (p = 0,02) e diminui??o na atividade da CAT (p = 0,04). O HIIE n?o alterou a viabilidade das PBMC, mas a frequ?ncia de c?lulas CD4 e CD19, positivas para os marcadores CD25 e CD69, foi menor ap?s o exerc?cio. Contudo, como foi observada redu??o na frequ?ncia de c?lulas CD4+ e CD19+, a redu??o da frequ?ncia de express?o de marcadores de ativa??o refletiu a redu??o do n?mero de c?lulas, e n?o da resposta ao est?mulo superantig?nico. Nossos resultados mostram portanto, que apesar do HIIE promover desequil?brio redox nas PBMC a resposta dos linf?citos ao est?mulo superantig?nico n?o ? alterada. A redu??o da resposta proliferativa ?, provavelmente, reflexo da altera??o da distribui??o das subpopula??es linfocit?rias em decorr?ncia do HIIE. / Disserta??o (Mestrado) ? Programa Multic?ntrico de P?s-gradua??o em Ci?ncias Fisiol?gicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT High-intensity interval exercise (HIIE) is characterized by brief and repeated intense exercise sessions, interspersed with periods of rest or low intensity exercise. This exercise modality has gained much attention in recent years, although little is known about its effects on immune function and cellular redox state. This study evaluated the effect of HIIE on the redox state and lymphocyte function in sedentary young males. This work was divided in two studies. The firsty study evaluated the effect of HIIE on peripheral blood mononuclear cells (PBMC) proliferation, cytokine production and redox status. The second study evaluated the effect of HIIE on lymphocyte viability and activation markers expression. HIIE was performed on cycloergometer, and consisted of eight series of 1 min at 90-100% of peak power, with 75 seconds of active recovery, at 30W, between sets. Venous blood was collected before, immediately after and 30 minutes after HIIE. For cell proliferation evaluation by flow cytometry, PBMC were stained with Carboxyfluorescein Succinimidyl Ester (CFSE) (10 mM) and stimulated with the superantigen SEB (100 ng/ml) for 5 days at 37? C, 5% CO2. IL-2 and IFN-? secretion in response to SEB stimulation for 18 hours was assessed by ELISA. The cellular redox status was assessed by measuring the activity of catalase (CAT) and superoxide dismutase (SOD), the concentration of thiobarbituric acid reactive substances (TBARS) and reduced glutathione content (GSH). To assess cellular viability, using flow cytometry, cells were labeled with anti-Annexin V-FITC and propidium iodide. To analyze lymphocyte activation, by flow cytometry, PBMC were stimulated with SEB for 18 hours, and then stained with fluorescent antibodies directed against CD4, CD8, CD19, CD25 and CD69. One-way or two-way ANOVA was employed for statistical analyzis, with ? ? 0.05. Lymphocyte proliferation was reduced (p = 0.01) after HIIE, despite increased IL-2 concentration (p = 0.02), and HIIE also induced PBMC redox imbalance characterized by increased TBARS concentration (p = 0.02) and decreased CAT activity (p = 0.04). PBMC viability was not affected by HIIE, but the frequency of CD4+CD25+/CD69+ and CD19+CD25+/CD69+ cells in response to SEB stimulation was lower after exercise. However, as CD4+ and CD19+ frequencies were reduced, reduced activation markers expression was a consequence of cell number reduction. Our results therefore show that, although HIIE induced redox imbalance, lymphocyte response to superantigen stimulation was not affected. Reduced lymphocyte proliferative response after HIIE is probably due to modifications in lymphocyte subpopulations distribution.