Bibliography: leaves 246-280. / xxi, 280 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Identifies that exercise-induced hypoxemia occurs at a much lower exercise intensity than reported previously and that exercise mode changes the severity of the phenomonen. / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 1999

Optimising recovery post-game or post-training could provide a competitive advantage to an athlete, especially if more than one bout of exercise is performed in a day. Active recovery is one common method that is thought to enhance the recovery process. Another recovery method that is gaining popularity is water immersion. The objective of this thesis was to analyse whether these two recovery methods provided greater recovery from explosive exercise than passive recovery. A physiological rationale that may explain the possibility of enhanced recovery with water immersion was initially investigated. The literature surrounding active recovery, water immersion and passive recovery on strength, cycling, running and jumping was then examined. Following these reviews an experimental study was conducted investigating the effects of water immersion, active recovery and passive recovery conducted after repeated bouts of explosive exercise. The rationale for active recovery post-exercise is that during intense exercise, fluid from the blood is forced into the working muscles due to the increase in mean arterial pressure, which increases muscle volume and decreases blood plasma fraction. Active recovery reduces this exercise induced edema and, with an associated increase in blood flow throughout the body, may increase the metabolism of waste substrates produced during exercise. Researchers have observed this increased substrate metabolism with reductions in post-exercise blood lactate accumulation following active recovery. Water immersion would appear to cause a similar physiological response to active recovery without the need to expend extra energy. When a large portion of the body is immersed, hydrostatic pressure acts on the body's fluids within the immersed region. Fluids from the extravascular space move into the vascular system reducing exercise-induced increases in muscular volume and reducing soft tissue inflammation. Additionally, blood volume increases and is redistributed towards the central cavity, which in turn increases cardiac preload, stroke volume, cardiac output, and blood flow throughout the body. Cardiac output increases in relation to the depth of immersion and have been observed to increase by as much as 102% during head-out immersions. These cardiovascular responses occur without any increase in energy expenditure. If extra-intravascular fluid movement is enhanced, then the movement and metabolism of waste substrates could increase. Observations of increased post-exercise blood lactate clearance with water immersion would support this theory. Most methodologies studying the performance benefits of active recovery and water immersion suffer many limitations. These limitations often consist of the experimental time schedule not replicating what is likely to occur in a practical situation, no isolation of water temperature and hydrostatic pressure effects, and lack of a sport-like exercise consisting of repeated expressions of explosive power. Light-intensity active recovery and water immersion do not appear to be detrimental to performance, but neither does there appear to be enough evidence to claim they are beneficial. Effects of active recovery and water immersion would seem to be trivial to small, with any benefits more likely following multiple bouts of high-intensity exercise and recovery or following muscle damaging exercise. There may be a link between blood plasma fraction and performance, however, evidence is inconclusive. Given these issues and limitations the aim of this research was to investigate whether combinations of active recovery, water immersion and passive recovery could maintain peak power and work during subsequent bouts of explosive exercise. We also investigated whether there was any difference in subjects' blood plasma faction and perceived fatigue between the recovery modes. A cross-over experiment was conducted on seven subjects over four weeks. On the same day of each week subjects performed three sessions of maximal jumping, each two hours apart, followed by a different recovery method. Each jump session consisted of three sets of 20 maximal jumps repeated every three seconds, with a minute's rest in-between. Immediately following the jumping subjects performed 10 minutes of either (A) active recovery on a cycle ergometer followed by seated rest, (I) immersion to the gluteal fold in 19°C water followed by seated rest, (AI) active recovery followed by immersion, or (P) seated passive rest. Jumping was conducted on an instrumented supine squat machine that allowed the measurement of total peak power and total work. Pre-jump, post jump and post-recovery blood was taken and the percentage of blood plasma fraction calculated. Perceived leg fatigue was also measured at these times. Observed differences in total peak power and total work between the recovery modes were non-significant. No differences were observed in the change of blood plasma fraction between the recovery modes or perceived fatigue. One reason for any lack of difference between the recovery modes may have been the brevity of the recovery time. Research that has observed significant benefits of active recovery and water immersion compared to passive recovery have used recovery times greater of 15 minutes or more. Additionally, changes in blood plasma fraction between active recovery, water immersion and passive recovery have not been apparent until at least 10 minutes post-recovery in previous research. Alternatively, rather than brevity, it may be that active recovery or water immersion simply does not provide any benefit to performance recovery. Overall there is a meagre amount of research into active recovery, water immersion and passive recovery. Further research that incorporates a variety of exercise and recovery protocols is required.

[Truncated abstract] Exercise is generally recommended for individuals with type 1 diabetes mellitus since it is associated with numerous physiological and psychological benefits. However, participation in exercise can also increase the risk of experiencing severe hypoglycaemia both during exercise and recovery. Unfortunately, existing guidelines to minimise the risk of exercise-induced hypoglycaemia are often general and fail to take into account that different precautions are required for exercise of varying type, duration and intensity. Specifically, there are no evidence-based guidelines for safe participation in intermittent high-intensity exercise (IHE), which characterises the activity patterns of most team and field sports, manual labour occupations and spontaneous play in children. This is because the response of blood glucose levels to this type of exercise is not known. Consequently, the purpose of this thesis was to investigate the glucoregulatory responses to IHE that replicates the high-intensity work-to-recovery ratios observed in intermittent sports in individuals with type 1 diabetes, in order to assess the associated risk of hypoglycaemia. The first study of this thesis examined the effect of the repeated bouts of high-intensity exercise that characterise IHE compared to remaining inactive, on blood glucose and glucoregulatory hormone levels in individuals with type 1 diabetes. Eight healthy individuals with type 1 diabetes were tested on two separate occasions during which either a 20 minute rest (CON) or an IHE protocol designed to simulate the activity patterns of team sports was performed (repeated 4 second sprints every 2 minutes). ... During the second hour of recovery, Ra and Rd returned to baseline following MOD, but remained elevated after IHE. These changes in Ra and Rd were consistent with a lower glucose infusion rate (GIR) during early recovery from IHE and a higher GIR after 2 hours of recovery compared to MOD. In conclusion, the repeated bouts of high-intensity exercise associated with IHE stimulate a more rapid and greater increment in Ra during exercise and attenuate glucose Rd during early recovery. These findings assist in explaining, in part, the previous observation that the risk of hypoglycaemia might be lower during IHE and early recovery compared to MOD. Overall, the findings of this thesis have implications for current recommendations aimed at managing blood glucose levels during and after exercise to avoid hypoglycaemia.

Whole Body Vibration (WBV) training is a new addition to the field of Exercise and Sports Science and has been developed for the use in strength and conditioning exercises. With the introduction of this new mode of exercise, the study focused on comparing the strength gaining effect of WBV training versus conventional resistance training. The study was conducted in a descriptive, exploratory manner utilizing a quasi-experimental approach with a three group comparison pre-test-post-test design consisting of an experimental-, comparison- and control group. Convenience and snowball sampling were used to select 43 male and female healthy, sedentary volunteer participants. The research focused on reviewing the contribution that each mode of training offers to increase strength in the upper leg and underlines the important physiological adaptations that the human body undergoes to bring about an increase in muscle strength. Both the whole body vibration and land-based resistance groups trained three times a week over an eight week intervention period. Exercises were performed with progressive increments in the frequency, amplitude and duration for the WBV- and in workload, number of sets and repetitions for the conventional resistance training program. The control group remained sedentary throughout the duration of the study. The dependent variables of peak torque flexion and extension of the knee joint in both legs were analyzed using descriptive and inferential statistics. Analysis of covariance (ANCOVA) was done to determine intra-group differences. Post-hoc analysis in the form of Scheffé’s test was done to determine and compare inter-group differences. Practical significance was indicated by means of Partial eta2 The analysis of the results revealed significant strength increases in both conventional resistance training and WBV for most of the dependent variables, except for peak torque extension, where the WBV group did not increase significantly. Based on these results, it can be concluded that both modes of conventional resistance and whole body vibration increased selected dependent variables for upper leg strength in previously inactive individuals.

To investigate the relationship between pulmonary diffusion limitation, ventilation-perfusion (VA/Q) mismatch, pulmonary transit times (PTT) and pulmonary gas exchange during exercise, 10 highly trained male athletes (age=26.4±4.4 years, Height=185.5±5.3cms, Weight=78.2±8.6 kg, V 02max=5.15±0.521-min-1) under went exercise testing at rest (R) and 150W, 300W and maximal exercise (372±22W), corresponding to an oxygen consumption (V0₂) of 0.41±0.09, 2.16±0.17, 4.32±0.35 and 5.13±0.50 1-min-1respectively, while trace amounts of six inert gases were infused via a peripheral vein. Arterial blood samples, mixed expired gas samples and metabolic data were obtained. Observed alveolar arterial difference ([A-a]D0₂(0)was calculated according to the alveolar gas equation. Indices of VA/Q mismatch: LogSDi and Log SDa and predicted [A-a]D0₂([A-a]DO₂(p)) were derived from 50 compartment model analysis of retentions and excretions of the inert gases. Additional indices of '/A/I,) mismatch: DISPR*, DISPE and DISPR*_E and inert gas alveolar difference ([A-a]D, R(A-a)D and E(A-a)D) were obtained directly from the inert gas data. One to two weeks later, the subjects underwent first pass radionuclide angiography using a Siemens ZLC wide field of view gamma camera. Following in vitro labeling with 99mTechnecium, 5-10 ml of the subject's blood, containing 10-20 mCi of activity, were injected at rest. First pass and post-static data were obtained on an ADAC 3003 computer and cardiac output was calculated using the Stewart Hamilton equation. PTT was determined using deconvolution and centroid methods. Gated radionuclide angiography was then performed at rest, 150, and 300W. On a separate occasion, first pass cardiac outputs and pulmonary transit times were obtained at maximal exercise. Mean arterial partial pressure of 0₂ (Pa0₂) decreased significantly from rest to 150W , and from 150 to 300W to a low value of 86±9 torn, before increasing to near resting values at maximal exercise. [A-a]D0₂(3) increased across each exercise levels however only the increase from 150 to 300 W was significant. The overall and perfusion-related indices of VA/Q mismatch showed a significant increase with exercise, mainly as a result of increasing perfusion of areas of high VA/Q [A-a]D0₂(0 was greater than predicted, becoming significant during heavy exercise, indicating diffusion limitation. Cardiac output increased from 6.9±0.9 1-min-1 (R) to 25.2±2.5 1-min-1 at 300W and 33.3±3.7 1-min-1 at maximal exercise. End diastolic volume increased from R to heavy exercise (p < 0.001), accompanied by a decrease in end systolic volume (p =0.05). Stroke volume and ejection fraction also increased significantly from R to 300W (p <0.001). Deconvolution PTT decreased from 9.32±1.41 s at rest to 2.91±0.30 s during max exercise and was highly correlated with centroid PTT both at rest (r=0.99, p<0.001) and during maximal exercise (r=0.96, p<0.001). PTT during maximal exercise was significantly correlated with Pa0₂ (1=0.65, p<0.05) and [A-a]D0₂(0)_[A-a]D0₂(p) (r=-0.60, p<0.05). Calculated pulmonary blood volume increased during maximal exercise by 57% over resting values to over 25% of total blood volume and when corrected for body surface area correlated significantly with Pa0₂ (r=0.69, p<0.05). There was a significant correlation between (A-a)D, PTT, the ventilatory equivalent for CO₂ and Pa0₂ during maximal exercise (r=0.94, p<0.01) allowing prediction of over 80% of the variance in Pa0₂ between subjects. These data indicate that highly trained athletes develop VA/Q mismatch accompanied by diffusion limitation during maximal exercise. Observed decrease in Pa0₂2 during high intensity exercise is the result of a complex interaction between VA/Q mismatch, hypoventilation and diffusion limitation secondary to shortened pulmonary transit. / Graduate and Postdoctoral Studies / Graduate

During pregnancy, body fat stores increase in part to subsidize the high energy cost of lactation. One effect of exercise, on the other hand, is to lower percent body fat. The effect of exercise during pregnancy and lactation on body fat, and on body composition in general, is not well documented. There is also a paucity of data on the effect of exercise during these physiological states on food intake and body weight. If exercise during pregnancy decreases body fat stores, would lactation performance subsequently be compromised? This study was designed to determine the effect of moderately strenuous aerobic exercise, during rat pregnancy and lactation, on food intake, body weight, body composition and lactation performance. Virgin female Sprague-Dawley rats were divided into exercised (n=40) and sedentary (n=40) groups. Exercising rats were trained over three weeks to run on a treadmill at 30 m/min, 2 hours/day, 5 days/week. Within each group, two subgroups were then mated and three subgroups remained as virgin age controls (n=8 per subgroup). Of the mated subgroups, one was terminated within 24 hours of parturition and the other on day 14 of lactation. Subgroups of virgin sedentary and exercising controls were terminated at times corresponding to each of mating, parturition and day 14 of lactation of mated animals. Carcasses were assayed for fat, water, ash and protein. Ad libitum food intake and body weight were monitored throughout the study, as was the weight gain of pups of lactating dams. MANOVA showed the effect of activity to be significant on food intake at week three of training and during the pregnancy period (p<0.00l) and at week one (p<0.0l) and two (p<0.05) of lactation. The effect of activity was highly significant (p<0.00l) on body weight from week three of training and throughout the pregnancy and lactation periods, as well as on the percent fat, water and ash of the rat carcasses. Post hoc multimean comparisons (Scheffe) at the p<0.05 level revealed that exercise resulted in a significant increase in the food intake of virgin rats, and nonsignificant increases in the food intake of pregnant and lactating rats. Body weights of virgin, pregnant and lactating exercising rats were significantly greater than their respective sedentary controls. Despite their heavier body weights and greater food intake, the estimated carcass energy content of exercising animals was lower than that of sedentary animals. This finding was reflected in the carcass composition whereby exercising rats, whether virgin, pregnant or lactating, contained consistently less fat and more water than sedentary controls. At parturition, pregnant animals contained significantly less fat, more water and more ash than sedentary pregnant controls. After 14 days of lactation, there were no significant differences in carcass composition between exercising and sedentary dams. However, lactating rats, whether exercising or sedentary, catabolized approximately 50 percent of the body fat present at parturition. Pup weight gains were not significantly different between exercising and sedentary dams. From these findings it was concluded that the effect of exercise during pregnancy and lactation on food intake, body weight and body composition was comparable to its effect in non-gravid rats. Moderately strenuous exercise during pregnancy prevented the increase in body fat deposition normally present at this time. Despite these depleted fat stores, the energy supplied by the mobilization of the remaining fat and the increase in food intake was adequate to support normal pup growth. / Land and Food Systems, Faculty of / Graduate

In order to test the effect of artificially induced alkalosis and acidosis on the appearance of blood lactate and work production, six well-trained oarsmen (age= 23.8 ±2.5 wt = 82.0 ±7.5kg.) were tested on three separate occasions after ingestion of 0.3 gm/kg body wt. NH4C1 (acidosis) , NaHC03 (alkalosis) or a placebo (control). Blood was taken from a forearm vein immediately prior to exercise for determination of pH and bicarbonate (HC03). One hour following the ingestion period, subjects rowed on a stationary ergometer at a pre-determined sub-maximal rate for 4 minutes, then underwent an immediate transition to a maximal effort for 2 minutes. Blood samples from an indwelling catheter placed in the cephalic vein were taken at rest and every 30 seconds throughout the 6 minute exercise test, and every 3 minutes during a 30 minute passive recovery period. Pre-exercise blood values demonstrated significant differences (p<0.01) in pH and HC03 in all three conditions. Work outputs were unchanged in the submaximal test and in the maximal test (p>0.05), although a trend toward decreased production was evident in the acidotic condition. Analysis of exercise blood samples using ANOVA with repeated measures revealed that the linear increase in blood lactate concentration([BLA]) during control was significantly greater than acidosis (p<0.01), although [BLa] during alkalosis were consistently elevated above control there was no significant difference in the linear trend (p>0.05). During recovery, there was no significant difference in the rate of lactate disappearance amongst the three conditions. It was concluded that under this protocol artificial manipulation of the acid-base status of the blood does not significantly influence work production despite significantly reduced [BLa] during acidosis. The inability of these pH changes to alter exercise performance emphasizes the relative importance of the intracellular and the extracellular buffer systems in well trained athletes. / Education, Faculty of / Curriculum and Pedagogy (EDCP), Department of / Graduate

The purpose of this study was to assess the energy demands of a 2,000 meter race simulation (RS) for national level oarswomen; as evaluated on a rowing ergometer (RE). A Progressive Intensity Test (P.I.T.) was also performed on the RE to further evaluate the RS. Six national level oarswomen (X values: age= 24.5 yrs., ht= 179 cm, wt= 75 kg), all current national team candidates (1988), participated in this investigation. A 6 1/2 minute tape recorded water race was used to execute a 2,000 meter RS on a Dr. Gjessing Ergorow ergometer. The tape recording was an actual race tape that was respliced to last exactly 6 1/2 minutes. Metabolic and respiratory exchange variables were continuously monitored by an open circuit method, utilizing a Beckman Metabolic Measurement Cart interfaced on-line with a Hewlitt Packard 3052A data acquisition system. The energy demands were calculated from metabolic variables, total oxygen cost and the analysis of excess post-exercise oxygen consumption (recovery V0₂).Results indicated a mean V0₂max. of 3.85 1.min⁻¹ , mean net V0₂ of 24.48 1 and a mean recovery V0₂ of 4.92 1. This represented the aerobic cost of the event at approximately 80% or 4/5ths of the total energy cost while the anaerobic contribution was approximately 20% of l/5th of the total energy cost. During the RS, V0₂ values rapidly increased to 90% of mean V0₂max. (3.85 l.min⁻¹) in the first two minutes. Mean max. VE (BTPS) RS value was 122.4 1.min⁻¹. VE plateaued after two minutes and remained around 90% of P.I.T. mean max. VE for the final 4 1/2 minutes. Mean max. excess C0₂ for RS was 19.81 ml.kg⁻¹.min⁻¹. The average maximal heart rate, as recorded in the RS was 192.8 bpm. The results of this study indicate the high aerobic demands and tremendous exercise intensity involved in the 2,000 meter RS. / Education, Faculty of / Curriculum and Pedagogy (EDCP), Department of / Graduate

This thesis presents two separate experiments concerning aerobic and anaerobic amphibian energetics. The first study (Part I) investigates differential alterations in the kinetics of the anaerobic enzyme lactate dehydrogenase, in nine species of anurans with varying natural histories. The second study (Part II) studies changes in different anaerobic and aerobic physiological parameters in a single species (Rana catesbeiana) after six weeks of chronic exercised, unexercised-captive and for field frogs. The common theme of these two experiments involves the potential for physiological changes due to environmental stress. Part I examines different species to infer a possible evolutionary relationship to environmental stresses at the molecular level of lactate dehydrogenase. Part II comprises the effects on captivity on aerobic and anaerobic capacities of R. catesbeiana,

The relationship between training intensities and fitness gains was selected as a topic for analysis in this study. Forty-six college men served as subjects in one of three groups: a jogging, or moderate intensity, training group, a running, or high intensity, training group, or a control group. All subjects were pre- and post-tested on the Astrand Bike Ergometer Work Test where working heart rates were measured and recorded. The two training groups participated in at least thirteen training sessions between these testing days, in which two miles were covered at the correct intensity level for each subject at each training session. A review of the literature revealed that most researchers embraced the concept of a minimal threshold of training stimulus needed to be met or exceeded by subjects for significant cardiovascular fitness gains to occur. Conclusions about the absolute level of this threshold varied from a low of 120 heartbeats per minute to at least 150 heartbeats per minute, depending apparently upon the experimental evidence each researcher had gathered. The author hypothesized that there existed a continuum of training stimuli such that training at higher intensities would produce larger heart rate decrements (a cardiovascular fitness index) than at moderate training intensities, but that moderate intensities would also produce significant gains. Furthermore, wide variations in heart rate decrements were expected to be observed within any one group, possibly indicating differences in initial fitnesses of subjects. The factual results of the study were: Both experimental groups and the control group exhibited significant decrements in heart rates from pre-test to post-test although the experimental groups' gains were significant at a higher confidence level. Explanations were posited about the possible factors which might have unpredictably caused the control group to have shown significant improvement. The moderate intensity training group exhibited a larger decrement in heart rate than the high intensity training group, although the difference was not statistically significant. There, indeed, was a wide variation of heart rate responses among individuals within any one group, possible indicating initial fitness differences. In light of the results derived from this study, the author can only conclude: Significant decreases in submaximal workload heart rates may be expected to be observed in American college men after training at moderate to high intensity levels (150 beats per minute or higher) for relatively short training periods (two days per week for eight weeks). Large fluctuations in fitness responses among subjects within any one training group can be expected, due probably to individual differences in initial fitnesses. The task of training large numbers of subjects at specified intensity levels within a fairly realistic physical education setting seems to have been a fruitful approach.