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京都大学 / 新制・課程博士 / 博士(農学) / 甲第23946号 / 農博第2495号 / 新制||農||1090(附属図書館) / 学位論文||R4||N5381(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 舘野 隆之輔, 教授 北島 薫, 教授 德地 直子 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

The purpose of this research was to determine whether all-purpose flour could be partially replaced with locust bean gum (LBG) and guar gum, and produce an acceptable bread product. A pilot study determined that up to 4% gum replacement for flour was feasible. All bread treatments were evaluated objectively by standing height, texture, color, and cell size. Moisture determinations were obtained for each bread variation. Sensory quality was examined by consumer testing. The amount of heat required to break the hydrogen bonds in amylopectin, indicative of bread staling, was measured by differential scanning calorimetry (DSC) for all bread treatments. Two percent LBG replacement significantly increased standing height. Firmness of bread increased with an increase in gum; the 4% guar bread was significantly firmer. Crumb color was not significantly different for any of the five bread treatments. Crust color, however, was significantly lighter for the control in comparison to the 2 and 4% guar, and 4% LBG breads. Two percent guar produced a more even cell size distribution throughout the bread crumb. For all 5 bread formulations moistures were not significantly different. Sensory evaluation determined a significant difference between the control and 4% LBG. The 4% LBG bread was preferred, although not significantly. Both gums were found to retard bread staling and 2% LBG was the most effective in lengthening the shelf life of the bread product. Objective and sensory evaluation indicated both gums produced acceptable bread products for consumer consumption and possible use in further research. / Master of Science

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This study provides chemical confirmation for the use of resinous plant exudates in mortuary contexts in Roman Britain. Analysis of amorphous masses, adhering residues and grave deposits using gas chromatography-mass spectrometry has revealed terpenoid biomarkers in sixteen inhumation and two cremation burials. The natural products characterized include European Pinaceae (conifer) resins, Pistacia spp. (mastic/terebinth) resins from the Mediterranean or the Levant and Boswellia spp. (frankincense) gum-resins from southern Arabia or eastern Africa. In addition, traces of a balsamic resin, probably Liquidambar orientalis, have been identified. A correlation between the use of these exotic exudates and interment in substantial, often multiple, containers with high-quality textiles and grave goods was observed. Theoretical consideration of this imported rite illuminates the multiplicity of roles played by resins/gum-resins in the mortuary sphere. The material properties of these highly scented substances speak to the biological reality of the decomposing body and to the socially constructed identity of the individual. On a practical level, they acted as temporary preservatives and masked the odour of decay. As social signifiers, they denoted the status of the deceased and promoted remembrance through conspicuous consumption and sensory impact. Encoded with ritual meaning, they purified the body and facilitated the final rite of passage to the afterlife. The recovery of these resinous traces provides us with new insights into the treatment of the body in the Roman period and establishes fresh links between the remote province of Britannia and the remainder of the Empire.

Diabetic retinopathy (DR) is becoming a global health concern, which causes the loss of vision of most patients with the disease. Due to the vast prevalence of the disease, the automated detection of the DR is needed for quick diagnoses where the progress of the disease is monitored by detection of exudates changes and their classifications in the fundus retina images. Today in the automated system of the disease diagnoses, several image enhancement methods are used on original Fundus images. The primary goal of this thesis is to make a comparison of three of popular enhancement methods of the Mahalanobis Distance (MD), the Histogram Equalization (HE) and the Contrast Limited Adaptive Histogram Equalization (CLAHE). By quantifying the comparison in the aspect of the ability to detect and classify exudates, the best of the three enhancement methods is implemented to detect and classify soft and hard exudates. A graphical user interface is also adopted, with the help of MATLAB. The results showed that the MD enhancement method yielded better results in enhancement of the digital images compared to the HE and the CLAHE. The technique also enabled this study to successfully classify exudates into hard and soft exudates classification. Generally, the research concluded that the method that was suggested yielded the best results regarding the detection of the exudates; its classification and management can be suggested to the doctors and the ophthalmologists.

This study provides chemical confirmation for the use of resinous plant exudates in mortuary contexts in Roman Britain. Analysis of amorphous masses, adhering residues and grave deposits using gas chromatography-mass spectrometry has revealed terpenoid biomarkers in sixteen inhumation and two cremation burials. The natural products characterized include European Pinaceae (conifer) resins, Pistacia spp. (mastic/terebinth) resins from the Mediterranean or the Levant and Boswellia spp. (frankincense) gum-resins from southern Arabia or eastern Africa. In addition, traces of a balsamic resin, probably Liquidambar orientalis, have been identified. A correlation between the use of these exotic exudates and interment in substantial, often multiple, containers with high-quality textiles and grave goods was observed. Theoretical consideration of this imported rite illuminates the multiplicity of roles played by resins/gum-resins in the mortuary sphere. The material properties of these highly scented substances speak to the biological reality of the decomposing body and to the socially constructed identity of the individual. On a practical level, they acted as temporary preservatives and masked the odour of decay. As social signifiers, they denoted the status of the deceased and promoted remembrance through conspicuous consumption and sensory impact. Encoded with ritual meaning, they purified the body and facilitated the final rite of passage to the afterlife. The recovery of these resinous traces provides us with new insights into the treatment of the body in the Roman period and establishes fresh links between the remote province of Britannia and the remainder of the Empire. / Arts and Humanities Research Council (AHRC). / Vol. II, which contains supplementary material files, is not available online.

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A citricultura brasileira apresenta números bastante significativos e expressivos, refletindo a grande importância social e econômica que essa atividade agrícola e industrial tem para a economia do país. O Brasil se destaca como o maior produtor de laranja do mundo. As plantas cítricas podem ser produzidas e multiplicadas de várias formas e a enxertia é a forma de propagação vegetativa mais utilizada comercialmente no Brasil. Dentre os porta-enxertos mais empregados, sobressaem-se o citrumeleiro Swingle e a tangerineira Sunki. Apesar das vantagens presentes nesses dois genótipos, a produção deles está sendo limitada por doenças causadas por fungos e estraminipilas. Em citros, as diversas espécies de Phytophthora são responsáveis pelas doenças gomose e podridões, principalmente de radicelas. P. nicotianae é a espécie que mais afeta as plantas cítricas no Brasil. Não obstante a grande importância de P. nicotianae para os porta-enxertos, alguns mecanismos de resistência ou susceptibilidade ainda requerem mais investigações cientificas. O presente trabalho visa a elucidação de alguns mecanismos de resistência dos citros a P. nicotianae, por meio de estudos fisiológicos e bioquímicos dos porta-enxertos, além da ação de meios de cultura e da qualidade da luz no estudo da fisiologia desse estraminipila. Para isso realizou-se a detecção de alguns equivalentes de compostos fenólicos através da técnica HPLC e o comportamento do genótipo resistente frente à possível inibição da síntese de compostos fenólicos através do tratamento das raízes do porta-enxerto Swingle com Pro-Ca; avaliou-se a atração de zoósporos de P. nicotianae por exsudatos radiculares provenientes dos dois porta-enxertos com o uso de uma armadilha adaptada; verificou-se o comportamento do zoósporo, a motilidade e a zoosporogênese frente à ação de vários compostos fenólicos e testou-se a ação de diferentes meios de culturas e da qualidade da luz no crescimento micelial e na esporulação de P. nicotianae. Os resultados revelaram que a tangerineira Sunki possui maiores quantidades de equivalentes em apigenina que o citrumeleiro Swingle nos dois períodos de avaliação, para o HPLC. O porta-enxerto resistente apresentou mais equivalentes de ácido clorogênico que o genótipo susceptível. Quanto ao Pro-Ca, os equivalentes de compostos fenólicos em raízes de plântulas do citrumeleiro Swingle não diferiram daquelas plantas tratadas com água. O Pro-Ca não inibiu e não desativou a síntese de compostos fenólicos. O uso do anticorpo contra a elicitina \"α-plurivorina\" teve resultados intermediários no consumo de água por plântulas de tangerineira Sunki e a atividade zoosporicida dos compostos fenólicos apresentou resultados variáveis no comportamento da motilidade de zoósporos. Os compostos fenólicos escopoletina e tricetinpentametoxi conseguiram paralisar a motilidade e proporcionaram as menores porcentagens de zoosporogênese. O meio de cultura AA permitiu a maior TCMD, seguido de CA e V8-CaCO3-Ágar. Os maiores valores de esporulação foram encontrados nesses dois últimos meios, respectivamente. Todas as faixas de luzes (e a ausência de luz) estudadas no presente trabalho podem ser avaliadas para o crescimento micelial, porém o tratamento claro foi aquele que mais induziu a esporulação de zoósporos de P. nicotianae. / The Brazilian citrus industry has very significant and expressive numbers, reflecting the great social and economic importance of this industrial and agricultural activity has for the economy. Brazil stands out as the world\'s largest orange producer. Citrus plants can be produced and multiplied in various ways and the bud is the form of vegetative propagation more commercially used in Brazil. Among the most commonly used rootstocks, stand up the Swingle citrumelo and Sunki mandarin. Despite the advantages these two genotypes present, their production is being limited by diseases caused by fungi and estraminipilas. In citrus, all species of Phytophthora are responsible for diseases gummosis and fibrous root rot, especially. P. nicotianae is the kind that affects citrus plants in Brazil. Regardless of the great importance of P. nicotianae for rootstocks, some mechanisms of resistance or susceptibility still require further scientific investigation. This study aims to elucidate some mechanisms of resistance of citrus to P. nicotianae, through physiological and biochemical studies of rootstocks, beyond of action culture media and quality of light in the physiological study of this estraminipila. To this was carried out to detect certain equivalent of phenolic compounds by HPLC and genotype resistant behavior against the possible inhibition of the synthesis of phenolic compounds by treatment of the roots of Swingle rootstock with Pro-Ca; evaluated the zoospores attraction P. nicotianae by exudates from the two rootstocks using an adapted trap; it was the zoospore behavior, motility and zoosporogenesis to the action of various phenolic compounds and tested the action of different culture media and quality of light on mycelial growth and sporulation of P. nicotianae. The results for HPLC revealed that Sunki mandarin has larger amounts of apigenin equivalents Swingle citrumelo in that the two periods. The sturdy rootstock had more chlorogenic acid equivalents of the susceptible genotype. As for the Pro-Ca equivalent of phenolic compounds in Swingle seedling roots not differ from those plants treated with water. The Pro-Ca not inhibited and not disabled the synthesis of phenolic compounds. The use of the antibody against elicitin \"α-plurivorin\" had intermediate results in water consumption by Sunki mandarin seedlings and zoosporicide activity of phenolic compounds showed variable results in the behavior of zoospore motility. The scopoletin and tricetinpentametoxi phenolic compounds managed to paralyze the motility and provided the smallest percentages of zoosporogenesis. The culture medium AA allowed greater DMGR, followed by CA and V8-CaCO3-Agar. The highest sporulation values were found in the last two media, respectively. All lights tracks (and the absence of light) studied in this work can be evaluated for mycelial growth, but the treatment light was that more induced sporulation of zoospores of P. nicotianae.