The ultra-processing of food has become a much more important aspect of dietary patterns and dietary quality in terms of its impact on body weight, diet related diseases, health, and well-being in the past decades. NOVA is a set of guidelines developed that classifies diet quality by degree of food processing. The NOVA guidelines distinguish four categories: unprocessed /minimally processed foods; culinary ingredients; processed foods; and ultra-processed foods. Numerous studies have found an association of ultra-processed foods and health conditions such as obesity and metabolic syndrome. This study analyzed the associations between maternal diet quality as measured by NOVA and maternal anthropometric and neonatal body composition outcomes. The optimal method of nutrition intervention and education for this special population remains unknown; using NOVA may provide researchers with a different lens to assess diet quality and health care professionals with additional vocabulary to convey more tailored messages regarding optimal nutrition strategies for mother and offspring. Using data collected from a large randomized controlled intervention trial at pre and post intervention, this study aimed to compare the NOVA guidelines assessment of maternal diet quality to the parent study assessment of diet quality, the Healthy Eating Index (HEI), using statistical correlations. Secondly, this study aimed to look at the relationship of ultra-processed food intake to the maternal gestational weight gain experience using a logistic regression. Thirdly, this dissertation aimed to explore the relationship between maternal ultra-processed food intake and neonatal lean mass as measured by quantitative magnetic resonance (QMR) and fat free mass as measured by air displacement plethysmography (ADP: PEAPOD). In terms of maternal outcomes, the study found that NOVA and HEI were significantly correlated at pre intervention but not at post intervention. The odds of gaining excessive gestational weight decreased as maternal ultra-processed food intake increased - which was not in the hypothesized direction - when using study participant data. However, the odds of gaining excessive gestational weight increased as maternal ultra-processed food intake increased - which was in the hypothesized direction - when using the Institute of Medicine weight gain recommendations. Also, while obesity did not predict excessive gestational weight gain, those with obesity ultra-processed food intake did predict gestational weight gain. These various inconsistencies are likely due to the instability of the dietary intake data because only one 24 -hour dietary recall was obtained from mother. In addition, the mothers’ diets were very healthy to begin with, where ultra-processed food intake formed about 45% of calories both pre and post intervention, when the national average is 57%. Race was also significant predictors of gestational weight gain for the mothers. Being non-white significantly increased the odds of gaining excessively as did the interaction of having obesity and eating more ultra-processed foods. In terms of neonatal outcomes, findings from this study suggest that length and fat mass are significant predictors of lean mass in neonates. In terms of the impact of maternal ultra-processed food intake, the higher the consumption of ultra-processed food, the greater the neonatal lean mass, which this was not in the hypothesized direction. However, the association was minimal with very small beta weights and regression line, when plotted was quite flat, so that the finding is not clinically meaningful. It remains important to know whether maternal ultra-processed food intake influences gestational weight gain and the body composition of the neonate. Thus, future research should include using similar data analyses on a population with a more nationally representative diet, a larger sample size, and a more robust measure of dietary intake such as three 24-hour recalls. Given that a similar recent study found ultra-processed food to be highly predictive of maternal and neonatal outcomes, and many other studies have demonstrated that ultra-processed food is related to several health conditions in many countries that this study did not measure, it seems prudent for healthcare providers to take advantage of prenatal visits as a window of opportunity to encourage the consumption of unprocessed and minimally foods and help women make informed decisions regarding ultra-processed foods.

Thesis (MTech (Consumer Science: Food and Nutrition))--Cape Peninsula University of Technology, 2007 / Labels are the source of information about the contents of food products and must be correct so that consumers are not misled and can make informed product choices. However, food label information is often incorrect, misleading or just insufficient. The aim of this study was to determine the labelling errors and concerns that occurred in specific categories of the South African processed food market. Randomly selected food product labels (N=246) were evaluated that represented the selected categories of processed foods (N=7), namely: breakfast cereal (9%), savoury snacks (13%), sweet snacks (29%), non-refrigerated meals (7%), refrigerated meals (9%), soups and sauces (25%) and convenience desserts and baked goods (8%). A pre-tested labelling checklist was used to evaluate each food label according to the food labelling areas that could impact consumer health considering the current South African labelling regulations published in 1993, the draft of these regulations published in 2002, and the further new proposed draft regulations. Labelling errors found induded the use of prohibited statements and not identifying compound ingredients (19% and 12% of the products respectively). A labelling concern was also the lack of identification of the fatsloils used (61% of the products). Further concerns identified included the lack of additive-free and allergen-free claims. For example, significant differences (p<O.05) were found between the number of products claiming to be aclditive-free and those that could have made such ctaims but did not. A real concern was the listing of ingredients of unknown origin with allergenicity potential (80% of the products). The breakfast cereal category contained the most eneigy and nutrient claims and nutritional education information, with most errors identified in the categories refrigerated meals and soups and sauces, and most concerns in the category savoury snacks. There is scope for food labelling improvement, specifically in the areas of ingredient and allergen identification, additive-free claims and heaJth-related information provision.

by Cheung Chi-kong, Chu Hok-keung, Ting Wai-tong. / Thesis (M.B.A.)--Chinese University of Hong Kong, 1990. / Bibliography: leaf 83. / Chapter I. --- BACKGROUND --- p.1 / Introduction --- p.1 / The Origin of Chinese Medicine --- p.2 / A Definition of Chinese Medicine --- p.5 / A Survey --- p.6 / Chapter II. --- LITERATURE REVIEW --- p.7 / Chapter III. --- METHODOLOGY --- p.14 / Data Sources --- p.14 / Sample Design --- p.15 / Data Processing --- p.16 / Chapter IV. --- FINDINGS FROM THE STUDY --- p.17 / Chinese Herbal Drugs --- p.17 / Chinese Health Foods --- p.23 / Further Analysis --- p.29 / Chapter V. --- SUMMARY AND RECOMMENDATIONS --- p.37 / Summary --- p.37 / Chinese Herbal Drugs : Recommendations --- p.39 / Chinese Health Foods : Recommendations --- p.52 / APPENDIX --- p.65 / Profiles of Respondents --- p.65 / Questionnaire (English/Chinese Version) --- p.68 / BIBLIOGRAPHY --- p.83

Thesis. 1977. M.C.P.--Massachusetts Institute of Technology. Dept. of Urban Studies and Planning. / MICROFICHE COPY AVAILABLE IN ARCHIVES AND ROTCH. / Bibliography : leaf 75. / by Stephen Klein. / M.C.P.

This dissertation, Fresh from the Factory: Breakfast Cereal, Natural Food, and the Marketing of Reform, 1890–1920, challenges dominant depictions of industry and environmental activism as adversarial by investigating producers who sought to reform capitalism with a new consumer good. Cereal companies at the end of the nineteenth century became some of the first manufacturers to distribute ready-to-eat food to consumers nationwide. Breakfast cereal’s ubiquitous advertising spoke of the virtues of “natural food” made in some of the country’s most impressive factories. Aimed squarely at women, this advertising preached the virtues of machine-made “natural food” by associating it with nutritional science, religious imagery, and stereotypes about the closeness-to-nature of women and racialized people. Selling a vision in which people could “return to nature” without going anywhere, industrialists persuaded consumers to pursue communion with nature by buying and eating packaged breakfast food. Breakfast cereal manufacturers became some of the world’s largest food processors— and among its most widely-read nature writers, health authorities, and social reformers. Fresh from the Factory follows the production and promotion of cereal as it developed in the early twentieth century. The first chapter tracks the cereal industry’s emergence out of a natural food movement that warmed to mass commerce over the nineteenth century. This movement’s spokespeople claimed to alone know what God, interchangeable with Nature, wanted people to eat. God’s authority proved useful for breakfast cereal producers, too, in branding their goods as “natural.” Subsequent chapters follow breakfast cereal from nature to table. To sell natural food, cereal companies spread new definitions of nature. These depictions rarely included plants or farms, instead emphasizing factories as the source of breakfast food and distribution in packages as the key to its freshness; in company nature writing, it was electric power, machinery, and pasteboard boxes that best mimicked the Garden of Eden. As cereal reached the table, consumers, regulators, and writers embraced, criticized, or even litigated against the product. Men often satirized the expensive grains in garish boxes, but many women found in cereal a more promising cure for sick children and arduous housework than the country retreats then favored by literary nature writers. By the early 1900s, breakfast cereal had become an American staple food, altering the country’s relationship to nature, cities, and the consumer economy. The dissertation ends in the 1920s. By this point, the federal government did more to protect national health, more people bought prepared packaged foods, and vitamins and calories had ascended over religion-infused ideas about nutrition. Still, the breakfast cereal industry’s ideas of nature persisted, and so the dissertation concludes by reflecting on continuing links between reform, business, and nature. I intend for scholars across fields to find this dissertation useful in considering how industry and the environment shape each other and the capacity of capitalism to reform itself.

A wealth of support has shown higher levels of state and trait impulsivity can be found among those individuals prone to developing problematic eating behaviors and obesity. Thus, upon commencing the investigations in this thesis, it was hypothesized that impulsivity is an individual difference implicated in overeating behaviour. Increasing information indicates that there are divisions within impulsivity subtypes. Prior to this thesis, studies in the field of eating behaviour had not distinguished between subtypes of impulsivity. This was problematic because it limited researchers ability to describe how impulsivity is specifically involved in the perpetuation of overeating behaviour. The purpose of this Thesis was to provide a methodical inquiry into the relationship between impulsivity, and its relation with overeating behaviour. This objective was achieved by separating three prioritites, first to determine if impulsivity was higher in women who self report overeating, second to define differences between impulsivity classifications and determine if there was a consistnent pattern between self reported overeating and a relation to a subtype of overeating behaviour, and third to designate a specific impulsivity subtype to individuals who self report overeating behaviour. Six Experimental Chapters explored these three priorities. Two exploratory correlational/regression analysis were used to refine our ability to operationalize measures of self reported overeating and impulsivity (Chapters 2 and 5). Chapter 3 and Chapter 5 were devoted to assess the impact that ingestion of palatable food, and the violation of cognitive boundaries of restraint, have on subsequent impulsivity. The two remaining investigations were structured to assess the impact that environmental factors have on impulsive behaviour. In Chapter 4, a Controlled versus Unrestricted eating environment were manipulated to determine whether overeaters benefit from a structured breakfast meal prior to completing a battery of impulsivity tasks. In Chapter 7, anticipation for a rewarding food item was manipulated in two conditions. In this final Chapter, the impact that anticipation for rewarding food in self reported overeaters was assessed. The battery of impulsivity tasks in this thesis include the Balloon Analogue Risk Task (BART), The Go No Go task, the Matching Familiar Figures task (MFFT), and two versions of the Delay Discounting Task (DDT). Impulsivity was classified along a spectrum of Reward Reactivity versus Inhibition subtypes, based on Evenden´s (1999) classification of impulsive behaviour. Participants tendency to overeat was based on a dual classification of tendency to restrain eating (Three Factor Eating Questionnaire- Restraint) with tendency to overeat (Three Factor Eating Questionnaire-Disinhibition subscale). The outcomes of the five experimental investigations in this thesis demonstrated a reliable pattern by which participants with high Disinhibition scores had significantly more impulsive responses on the MFFT task. These results indicated that inhibition impulsivity is the clearest individual difference to be found between healthy volunteers who self-report overeating. The role that Inhibition Impulsivity plays in the perpetuation of overeating behaviour is illustrated and discussed in each Experimental Chapter.

This thesis challenges accounts of individual choice and responsibility where food consumption is concerned, beginning with a critique of government policies to address the health effects of over-consumption of food. Whilst research from psychology and economics has acknowledged the role of habit and automaticity in some behaviours, including eating, the resulting theory of behavioural economics and its operationalisation as ‘nudge' theory does not directly address class differences. I argue that sociological analysis can do so, and discuss the trajectory of social class in social theory in recent decades, bringing together the insights of several theorists to challenge both Giddens's concept of reflexivity and postmodern notions of consumerism as an equalising force. I demonstrate that social theory can provide a solid underpinning to behavioural economics, and at the same time show the weakness of its policy applications to healthy eating. Given the relative inattention to the structures that shape dietary ‘choices', in comparison to the study of behaviour, this thesis examines the nature of the food supply and traces how the food industry develops, markets and sites food, and the ways in which it engages, often interactively, with a highly segmented society. The production and targeted supply of processed foods of varying quality contributes to problematic food consumption, particularly among those of lower social status. This phenomenon is further evidenced by an epidemiological review outlining the food-health-class link. Subsequently, I conduct the first sociological analysis of food industry texts (representing food science, product development and marketing), revealing how actors within these disciplines articulate their role, function and concerns regarding current practice. A critique of marketing emerges from both practitioners and marketing academics, and I apply this and an expanded sociological critique to the role of the food supply in diet-related ill health, in which a social gradient is strongly apparent.

Sensory-specific satiety (SSS) is a significantly greater pleasantness decline for a consumed (Eaten) food, than foods that are tasted but not consumed (Uneaten). SSS occurs during consumption, reaches optimal magnitude immediately afterwards, and returns to baseline within two to three hours. The phenomenon is dependent on the sensory properties, rather than the energy or macronutrient content of the food. To the extent that an Uneaten food shares similar sensory properties with the Eaten food, the Uneaten food may be subject to pleasantness decline: a transfer effect. Repeated exposure to a food stimulus may alter liking in the long-term, through mere exposure, monotony, and dietary learning paradigms resulting in an association between the novel target food and either a known food stimulus, or a consequence of consumption. Novel foods are more susceptible to these effects than familiar foods, for which learned associations may have already formed. Repeated consumption alone does not modulate SSS, but to date such studies have not tested novel foods. Through six experiments this research explores the influences of long-term pleasantness changes of novel foods and the number and type of Uneaten foods present during SSS testing, on the magnitude of SSS for snack foods. While no evidence of mere exposure or dietary learning was found, and in some instances experiments failed to induce SSS, these negative results are likely due to methodological, and sometimes procedural issues in the design and conduct of experimental testing. Findings revealed SSS to be vulnerable to a number of procedural and methodological factors, such as: portion size; baseline novelty and pleasantness ratings; hunger; perceived ambiguity of measurement scales; and expectations raised by the type and number of Uneaten foods present during testing.

Orientador: Rogerio Vieites / Banca: Juliana Audi Giannoni / Banca: Elisangela Marques Jeronimo Torres / Resumo: O pêssego (Prunus pérsica (L) Batch) é um fruto altamente perecível, devido a sua elevada atividade metabólica. Este fruto é apreciado pelo consumidores do mercado nacional e internacional por suas agradáveis propriedades organolépticas (cor, odor e sabor). A utilização de técnicas pós-colheita possibilita conservar os atributos de qualidade dos frutos. O objetivo deste trabalho foi avaliar a conservação de pêssegos cv Tropic Beauty minimamente processados refrigerados, empregando diferentes atmosferas modificadas ativas. Os frutos após a colheita foram selecionados, lavados, cortados, homogeneizados e embalados em diferentes atmosferas modificadas ativas e armazenados sob refrigeração em câmara fria a 5±0,5 °C e 85 ± 5% UR. Foram analisados parâmetros fisico-quimicos (pH, acidez titulavel, sólidos solúveis, ratio, açúcares redutores e totais, perda de massa, taxa respiratória e cor) e bioquímicos (compostos fenólicos totais, atividade antioxidante pelo método DPPH, pigmentos, flavonoides, peroxidase, polifenoloxidase), além da análise sensorial. As analises foram realizados nos frutos em triplicata a cada 2 dias durante 10 dias. O delineamentos experimental utilizado foi o inteiramente casualizado (DIC) em esquema fatorial (6x5) com 3 repetições por tratamento. Os dados foram submetidos à análise de variância e as medias foram comparadas pelo teste de Tukey e regressão polinomial (p<0,05). As atmosferas compostas por 7% CO2 ou 8% CO2 + 4% O2 apresentaram os melhores resultad... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Peach (Prunus persica (L) Batch) is a highly perishable fruit, due to its high metabolic activity. This fruit is appreciated by consumers in the national and international market for its pleasant organoleptic properties (color, odor and flavor). The use of postharvest techniques can preserve fruit quality attributes. The aim of this research was to evaluate the conservation of freshly processed refrigerated peach cv Tropic Beauty, using different active modified atmospheres. The fruits after harvest were selected, washed, cut, mixed and packaged in different active atmospheres and stored under refrigeration in a cold room 5 ± 0.5 ° C and 85 ± 5% RH. Physical and chemical analyzes (pH, titratable acidity, soluble solids, ratio, reducing and total sugars, mass loss, respiratory rate, color) and biochemical analyzes (total phenolic compounds, antioxidant activity by DPPH method, pigments, flavonoids, peroxidase, polyphenoloxidase) and sensory analysis. The analysis were performed on the fruits in triplicate every 2 days for 10 days. The experimental design was completely randomized in a factorial scheme (6x5) with 3 replicates per treatment. Data were submitted to analysis of variance by Tukey test and polynomial regression (p <0.05). The atmospheres composed of 7% CO2 or 8% CO2 + 4% O2 presented better results regarding the conservation and maintenance of the quality of this fruit / Mestre

Wong Wai Mei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 175-192). / Abstracts in English and Chinese. / Declaration --- p.ii / Acknowledgements --- p.iii / Abstract --- p.iv / Abbreviation --- p.vi / Table of Contents --- p.vii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Section I --- The Making of Genetically Modified Organisms --- p.2 / Chapter 1.1 --- Conventional breeding in agriculture --- p.2 / Chapter 1.2 --- What is genetic engineering? --- p.4 / Chapter 1.3 --- Plant transformation --- p.5 / Chapter 1.3.1 --- Agrobacterium-mediated --- p.6 / Chapter 1.3.2 --- Direct gene transfer --- p.8 / Chapter 1.3.2.1 --- Microparticle bombardment --- p.8 / Chapter 1.3.2.2 --- Protoplasts --- p.9 / Chapter 1.3.3 --- Gene silencing --- p.10 / Chapter 1.4 --- Examples of genetically modified crops --- p.13 / Chapter 1.5 --- Foreign genes commonly found in transgenic plants --- p.14 / Chapter Section II --- Benefits and Environmental Concern of GMOs --- p.17 / Chapter 2.1 --- Mechanism of GMO --- p.17 / Chapter 2.1.1 --- Herbicide tolerant crops --- p.18 / Chapter 2.1.2 --- Insect resistant crops --- p.19 / Chapter 2.1.3 --- Delayed ripening crops --- p.20 / Chapter 2.1.4 --- Virus resistant crops --- p.20 / Chapter 2.2 --- Benefits of GMOs --- p.21 / Chapter 2.3 --- Impact of GM foods to human health and the environment --- p.22 / Chapter 2.3.1 --- Human health --- p.22 / Chapter 2.3.1.1 --- GM potatoes --- p.23 / Chapter 2.3.1.2 --- CaMV risks? --- p.24 / Chapter 2.3.1.3 --- Food allergy --- p.25 / Chapter 2.3.2 --- Environmental concerns --- p.26 / Chapter 2.3.2.1 --- Horizontal gene transfer --- p.27 / Chapter 2.3.2.1.1 --- Selectable marker genes --- p.27 / Chapter 2.3.2.1.2 --- Herbicide resistant genes --- p.29 / Chapter 2.3.2.1.3 --- Insect resistant genes --- p.29 / Chapter 2.3.2.2 --- Ecology --- p.30 / Chapter 2.3.2.2.1 --- Monarch butterfly --- p.30 / Chapter Section III --- Future developments of GMO --- p.32 / Chapter 3.1 --- Designer Food and engineered plants --- p.32 / Chapter 3.1.1 --- Insect resistance --- p.33 / Chapter 3.1.2 --- Viral resistance --- p.33 / Chapter 3.1.3 --- Fungal resistance --- p.34 / Chapter 3.1.4 --- Nutritional quality --- p.34 / Chapter 3.1.5 --- Modifications of oil composition --- p.35 / Chapter 3.1.6 --- Medical applications --- p.37 / Chapter 3.1.7 --- Environmental applications --- p.40 / Chapter 3.1.7.1 --- Tolerance to high salinity and drought --- p.40 / Chapter 3.1.7.2 --- Tolerance to frost --- p.41 / Chapter 3.1.7.3 --- Bioremediation --- p.42 / Chapter 3.1.7.4 --- Biodegradable products --- p.43 / Chapter Section IV --- Regulation of GMO --- p.44 / Chapter 4.1 --- The question of labeling --- p.44 / Chapter 4.1.1 --- Moral and ethical issues --- p.44 / Chapter 4.1.2 --- Animal welfare --- p.45 / Chapter 4.2 --- International practice in GMO labeling --- p.46 / Chapter 4.2.1 --- United States of America --- p.46 / Chapter 4.2.2 --- Canada --- p.48 / Chapter 4.2.3 --- European Union --- p.49 / Chapter 4.2.4 --- Australia and New Zealand --- p.50 / Chapter 4.2.5 --- Japan --- p.51 / Chapter 4.2.6 --- Republic of Korea --- p.52 / Chapter 4.2.7 --- China --- p.53 / Chapter 4.2.8 --- Taiwan --- p.53 / Chapter 4.2.9 --- Hong Kong --- p.54 / Chapter Section V --- Uses of crops --- p.56 / Chapter 5.1 --- Uses of crops --- p.56 / Chapter 5.1.1 --- Soybean --- p.56 / Chapter 5.1.2 --- Corn --- p.57 / Chapter 5.1.3 --- Tomato --- p.58 / Chapter 5.1.4 --- Potato --- p.59 / Chapter 5.1.5 --- Rice --- p.60 / Chapter 5.1.6 --- Rapeseed --- p.61 / Chapter 5.1.7 --- Oil --- p.62 / Chapter 5.2 --- "Food additives, hormones and flavourings" --- p.63 / Chapter Chapter 2 --- Materials & Methods --- p.65 / Chapter 2.1 --- Materials --- p.66 / Chapter 2.1.1 --- Growth media & agar --- p.66 / Chapter 2.1.2 --- Reagents for agarose gel electrophoresis --- p.67 / Chapter 2.1.3 --- Reagents for preparation of competent cells --- p.67 / Chapter 2.1.4 --- Reagents for measurement of DNA concentration --- p.68 / Chapter 2.1.4.1 --- Measurement of DNA concentration by PicoGreen --- p.68 / Chapter 2.1.5 --- Reagents for Southern hybridization --- p.68 / Chapter 2.2 --- Methods --- p.70 / Chapter 2.2.1 --- Restriction endonuclease digestion --- p.70 / Chapter 2.2.2 --- Agarose gel electrophoresis of DNA --- p.70 / Chapter 2.2.3 --- DNA recovery from agarose gel --- p.71 / Chapter 2.2.3.1 --- QIAquick® gel extraction --- p.71 / Chapter 2.2.4 --- Ligation of purified DNA fragment into vector --- p.72 / Chapter 2.2.5 --- Transformation --- p.72 / Chapter 2.2.6 --- Rubidium chloride method for making competent cells --- p.12 / Chapter 2.2.7 --- Plasmid DNA preparation --- p.73 / Chapter 2.2.7.1 --- Concert Rapid Mini Prep --- p.73 / Chapter 2.2.7.2 --- QIAprep® Miniprep --- p.74 / Chapter 2.2.8 --- Extraction of plant genomic DNA --- p.75 / Chapter 2.2.8.1 --- Qiagen DNeasy´ёØ Plant Mini Kit --- p.75 / Chapter 2.2.9 --- Southern Hybridization --- p.75 / Chapter 2.2.9.1 --- Denaturation --- p.76 / Chapter 2.2.9.2 --- Blot transfer --- p.76 / Chapter 2.2.9.3 --- Pre-hybridization --- p.77 / Chapter 2.2.9.4 --- Synthesis of radiolabelled probe --- p.77 / Chapter 2.2.9.5 --- Hybridization of radiolabelled probe on filter --- p.77 / Chapter 2.2.9.6. --- Detection of hybridized probes --- p.78 / Chapter 2.2.10 --- Measurement of DNA concentration --- p.78 / Chapter 2.2.10.1 --- Determination of DNA on EtBr stained gel --- p.78 / Chapter 2.2.10.2 --- Determination of DNA by UV spectrophotometer --- p.78 / Chapter 2.2.10.3 --- Determination of DNA by PicoGreen --- p.79 / Chapter 2.2.11 --- DNA sequencing --- p.80 / Chapter 2.2.11.1 --- Automated sequencing by ABI Prism 377 --- p.80 / Chapter Chapter 3 --- PCR Diagnostics --- p.81 / Chapter 3.1 --- Applications of PCR to processed foods --- p.82 / Chapter 3.1.1 --- DNA quality --- p.82 / Chapter 3.1.2 --- PCR & Multiplex PCR --- p.83 / Chapter 3.1.3 --- Choice of primers --- p.84 / Chapter 3.1.4 --- Inhibitors --- p.84 / Chapter 3.2 --- Materials & Methods --- p.85 / Chapter 3.2.1 --- Selection of primers --- p.85 / Chapter 3.2.2 --- Amplification of target sequences --- p.86 / Chapter 3.2.3 --- Multiple amplification of target sequences --- p.87 / Chapter 3.3 --- Results --- p.88 / Chapter 3.4 --- Discussion --- p.93 / Chapter Chapter 4 --- Quality Control in GMO detection --- p.95 / Chapter 4.1 --- Standardization of pre- and post- PCR analysis --- p.96 / Chapter 4.1.1 --- General guidelines --- p.96 / Chapter 4.1.2 --- UV irradiation --- p.97 / Chapter 4.1.3 --- Inactivation protocols --- p.93 / Chapter 4.1.4 --- Positive and negative controls --- p.99 / Chapter 4.1.5 --- PCR verification --- p.99 / Chapter 4.1.6 --- Equipment decontamination --- p.100 / Chapter 4.2 --- Materials & Methods --- p.101 / Chapter 4.2.1 --- Selection of primers for external control --- p.101 / Chapter 4.2.2 --- Development of the external control --- p.101 / Chapter 4.2.3 --- Selection of primers for internal control --- p.103 / Chapter 4.3 --- Results --- p.104 / Chapter 4.4 --- Discussion --- p.107 / Chapter Chapter 5 --- DNA extraction from food samples --- p.110 / Chapter 5.1 --- Introduction --- p.111 / Chapter 5.2 --- Reagents and Buffers for DNA extraction from food samples --- p.112 / Chapter 5.2.1 --- Cetyltrimethylammonium bromide (CTAB) extraction method --- p.112 / Chapter 5.2.2 --- Organic-based extraction method --- p.113 / Chapter 5.2.3 --- Potassium acetate/sodium dodecyl sulphate precipitation method --- p.113 / Chapter 5.2.4 --- Hexane-based extraction method --- p.114 / Chapter 5.3 --- Weight and names of samples --- p.115 / Chapter 5.4 --- DNA extraction methods --- p.115 / Chapter 5.4.1 --- CTAB extraction method --- p.115 / Chapter 5.4.2 --- Qiagen DNeasy´ёØ plant mini kit --- p.116 / Chapter 5.4.3 --- Promega Wizard® genomic DNA purification --- p.116 / Chapter 5.4.4 --- Promega Wizard® Magnetic DNA purification system --- p.117 / Chapter 5.4.5 --- Promega Wizard® DNA Clean-Up system --- p.118 / Chapter 5.4.6 --- Qiagen QIAshreddrer´ёØ and QIAamp spin column --- p.119 / Chapter 5.4.7 --- Chelex-based extraction method --- p.119 / Chapter 5.4.8 --- Organic-based extraction method --- p.120 / Chapter 5.4.9 --- Nucleon PhytoPure extraction and purification method --- p.120 / Chapter 5.4.10 --- Potassium acetate/SDS precipitation method --- p.121 / Chapter 5.4.11 --- Hexane-based extraction method --- p.122 / Chapter 5.5 --- Results --- p.123 / Chapter 5.5.1 --- Comparison of eleven extraction methods --- p.123 / Chapter 5.5.2 --- Comparison of DNA extraction on selected methods --- p.125 / Chapter 5.6 --- Discussion --- p.132 / Chapter Chapter 6 --- Quantitative Analysis --- p.136 / Chapter 6.1 --- Introduction --- p.137 / Chapter 6.1.1 --- Chemistry of quantitative PCR --- p.138 / Chapter 6.1.2 --- PCR system --- p.140 / Chapter 6.2 --- Materials & Methods --- p.142 / Chapter 6.2.1 --- Design of primers and probes --- p.142 / Chapter 6.2.2 --- Methods --- p.145 / Chapter 6.3 --- Results --- p.146 / Chapter 6.3.1 --- Selection of primer/probe --- p.146 / Chapter 6.3.2 --- Primer optimization --- p.149 / Chapter 6.3.3 --- Quantitative analysis of real samples --- p.158 / Chapter 6.4 --- Discussion --- p.152 / Chapter Chapter 7 --- Conclusion --- p.168 / References --- p.175 / Appendix --- p.193