This thesis was about the relationship of the Factor V Leiden mutation and activated protein C resistance. I looked up patients with Leiden mutation and activated protein C resistance. I monitored the frequency of thromboembolism and miscarriages in the personal and family case history of patients. Subsequently, I looked up if other risk factors which affect clinical manifestations in patients with this mutation can be found.

Diploma thesis deals with design and implementation of manufacturing technology of a part which was given by company Frentech Aerospace s.r.o. Manufacturing technology is prepared for conditions of workshop of Department of Machining FME Brno UT (laboratory C2). Acquired knowledges are used for design of innovative manufacturing technology with cutting tools from company Pramet Tools, s.r.o. Manufacturing technologies of gained part are designed for alloy blank EN AW 6082. Technical-economical assessment of all manufacturing technologies is part of this thesis. Both of manufacturing technologies designed for laboratory C2 are assessed together and manufacturing technology given by company Frentech Aerospace s.r.o. is assessed alone due to using different blank material.

This master´s thesis applies to design of power and data wiring controlle by an intelligent wiring. The aim is to get to know the issue and design a project for family house that includes fhotovoltaic power station. In the introduction is a description of the inteligent wirings, its potential, topology, advantages or disadvantages towards standard electroinstallations,it also presents an overview of widely used intelligent wiring, especially Foxtrot. What follows is the subject itself for what is the project designated for. The next topic will be a presentation of complete electroinstallation and photovoltaic power station. It all ends with an economic evaluation of the whole project.

This thesis describes the construction and characterisation of an insulin-like growth factor (IGF-I)-binding single chain Fv (scFv) and the utilisation of this scFv as a model protein for the study of the application of DNA shuffling and ribosome display to antibody engineering. The variable domain genes were isolated from the hybridoma cell line producing the monoclonal antibody and successfully joined by PCR for the construction of the scFv, named anti-GPE. Sequencing of the gene revealed an unusually short heavy chain CDR2 region. The cloned scFv was expressed in E. coli and purified. Expression levels were low and the protein has poor solubility, most likely due to a reduction in folding efficiency caused by the abbreviated CDR2. The purified monomeric form of the protein was analysed for binding to IGF-I using surface plasmon resonance on the BIAcore 1000 with the specificity of the IgG version of the antibody for the three N-terminal residues of IGF-I - Gly-Pro-Glu - reproduced. The scFv's calculated dissociation constant of 3.68 µM is a low affinity for an antibody and is approximately 36-fold weaker than was calculated for the Fab version of the antibody, but it is concluded that the calculated affinity for the scFv was an apparent affinity that may be an underestimation of true affinity due to the presence of non-functional or misfolded scFv species within the gel-filtration purified monomer peaks. A mutant version of anti-GPE with residues inserted in the CDR2 to restore it to normal length produced a protein with improved expression and solubility characteristics while retaining IGF-I-binding. It was concluded that the short CDR2 was due to deletions generated during the somatic mutation process and a model for this is described. A ribosome display method using a rabbit reticulocyte lysate as a source of ribosomes was developed for specific selection of anti-GPE against IGF-I. Error prone PCR was used to produce a random point mutated library of anti-GPE (EPGPE). This was taken through several cycles of display and selection but selection for non-specifically binding scFvs was commonly observed. This was probably due to poor folding of ribosome-displayed proteins in the system used, possibly caused by the presence of DTT in the lysate and/or the low capacity of the anti-GPE framework to tolerate mutation while retaining stability. It is assumed misfolds, exposing hydrophobic regions, would have a tendency to non-specifically interact with the selection surface. Of the 64 EPGPE clones screened from four rounds of display and selection, many were shown to have poor or non-specific binding, but one scFv was characterised that was affinity matured 2.6-fold over anti-GPE wild type affinity for IGF-I. A DNA shuffling method was developed to produce libraries of chimaeric scFvs between anti-GPE and NC10 (anti-neuraminidase scFv) with the objective of isolating functional IGF-I-binding chimaeras. The NC10 scFv had its CDRs replaced with the anti-GPE CDRs prior to the shuffling to increase the likelihood of isolating IGF-I binders. Ribosome display was used for selection from the chimaera libraries. Selection strategies included elution of specific binders by GPE peptide and a GPE 10-mer peptide. Selection was also performed using IGF-I immobilised on a BIAcore sensorchip as a selection surface. Again, much non-specific selection was observed as seen for display of EPGPE, for what was expected to be the same reasons. Selected scFvs were genuinely chimaeric but with poor expression and solubility and mostly non-specific in their binding. One characterised selected chimaera, made up of three segments of each of the parental scFvs, was shown to bind specifically to IGF-I by BIAcore. Steps to improve the efficiency of the ribosome display system have been identified and are discussed.

This thesis explores motion trajectory-based approaches to recognize human actions in real-world, unconstrained videos. Recognizing actions is an important task in applications such as video retrieval, surveillance, human-robot interactions, analysis of sports videos, summarization of videos, behaviour monitoring, etc. There has been a considerable amount of research done in this regard. Earlier work used to be on videos captured by static cameras where it was relatively easy to recognise the actions. With more videos being captured by moving cameras, recognition of actions in such videos with irregular camera motion is still a challenge in unconstrained settings with variations in scale, view, illumination, occlusion and unrelated motions in the background. With the increase in videos being captured from wearable or head-mounted cameras, recognizing actions in egocentric videos is also explored in this thesis. At first, an effective motion segmentation method to identify the camera motion in videos captured by moving cameras is explored. Next, action recognition in videos captured in normal third-person view (perspective) is discussed. Further, the action recognition approaches for first-person (egocentric) views are investigated. First-person videos are often associated with frequent unintended camera motion. This is due to the motion of the head resulting in the motion of the head-mounted cameras (wearable cameras). This is followed by recognition of actions in egocentric videos in a multicamera setting. And lastly, novel feature encoding and subvolume sampling (for “deep” approaches) techniques are explored in the context of action recognition in videos. The first part of the thesis explores two effective segmentation approaches to identify the motion due to camera. The first approach is based on curve fitting of the motion trajectories and finding the model which best fits the camera motion model. The curve fitting approach works when the trajectories generated are smooth enough. To overcome this drawback and segment trajectories under non-smooth conditions, a second approach based on trajectory scoring and grouping is proposed. By identifying the instantaneous dominant background motion and accordingly aggregating the scores (denoting the “foregroundness”) along the trajectory, the motion that is associated with the camera can be separated from the motion due to foreground objects. Additionally, the segmentation result has been used to align videos from moving cameras, resulting in videos that seem to be captured by nearly-static cameras. In the second part of the thesis, recognising actions in normal videos captured from third-person cameras is investigated. To this end, two kinds of descriptors are explored. The first descriptor is the covariance descriptor adapted for the motion trajectories. The covariance descriptor for a trajectory encodes the co-variations of different features along the trajectory’s length. Covariance, being a second-order encoding, encodes information of the trajectory that is different from that of the first-order encoding. The second descriptor is based on Granger causality. The novel causality descriptor encodes the “cause and effect” relationships between the motion trajectories of the actions. This type of interaction descriptors captures the causal inter-dependencies among the motion trajectories and encodes complimentary information different from those descriptors based on the occurrence of features. The causal dependencies are traditionally computed on time-varying signals. We extend it further to capture dependencies between spatiotemporal signals and compute generalised causality descriptors which perform better than their traditional counterparts. An egocentric or first-person video is captured from the perspective of the personof-interest (POI). The POI wears a camera and moves around doing his/her activities. This camera records the events and activities as seen by him/her. The POI who is performing actions or activities is not seen by the camera worn by him/her. Activities performed by the POI are called first-person actions and third-person actions are those done by others and observed by the POI. The third part of the thesis explores action recognition in egocentric videos. Differentiating first-person and third-person actions is important when summarising/analysing the behaviour of the POI. Thus, the goal is to recognise the action and the perspective from which it is being observed. Trajectory descriptors are adapted to recognise actions along with the motion trajectory ranking method of segmentation as pre-processing step to identify the camera motion. The motion segmentation step is necessary to remove unintended head motion (camera motion) during video capture. To recognise actions and corresponding perspectives in a multi-camera setup, a novel inter-view causality descriptor based on the causal dependencies between trajectories in different views is explored. Since this is a new problem being addressed, two first-person datasets are created with eight actions in third-person and first-person perspectives. The first dataset is a single camera dataset with action instances from first-person and third-person views. The second dataset is a multi-camera dataset with each action instance having multiple first-person and third-person views. In the final part of the thesis, a feature encoding scheme and a subvolume sampling scheme for recognising actions in videos is proposed. The proposed Hyper-Fisher Vector feature encoding is based on embedding the Bag-of-Words encoding into the Fisher Vector encoding. The resulting encoding is simple, effective and improves the classification performance over the state-of-the-art techniques. This encoding can be used in place of the traditional Fisher Vector encoding in other recognition approaches. The proposed subvolume sampling scheme, used to generate second layer features in “deep” approaches for action recognition in videos, is based on iteratively increasing the size of the valid subvolumes in the temporal direction to generate newer subvolumes. The proposed sampling requires lesser number of subvolumes to be generated to “better represent” the actions and thus, is less computationally intensive compared to the original sampling scheme. The techniques are evaluated on large-scale, challenging, publicly available datasets. The Hyper-Fisher Vector combined with the proposed sampling scheme perform better than the state-of-the-art techniques for action classification in videos.

Submitted by Regina Correa (rehecorrea@gmail.com) on 2016-10-03T18:50:36Z No. of bitstreams: 1 DissNZB.pdf: 8463882 bytes, checksum: 493c4ee9ac0735d4fab21c75b50bef13 (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-10T14:21:16Z (GMT) No. of bitstreams: 1 DissNZB.pdf: 8463882 bytes, checksum: 493c4ee9ac0735d4fab21c75b50bef13 (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-10T14:21:24Z (GMT) No. of bitstreams: 1 DissNZB.pdf: 8463882 bytes, checksum: 493c4ee9ac0735d4fab21c75b50bef13 (MD5) / Made available in DSpace on 2016-10-10T14:21:33Z (GMT). No. of bitstreams: 1 DissNZB.pdf: 8463882 bytes, checksum: 493c4ee9ac0735d4fab21c75b50bef13 (MD5) Previous issue date: 2015-12-18 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Friction Riveting is an innovative spot joining technology for metal-polymer hybrid structures. This MSc thesis provided for the first time in the literature, a fundamental understanding on the Friction Riveting process for metal-thermoset composites joints. Joints of Ti-6Al-4V rivet and pultruded glass fiber reinforced thermoset polyester part were produced under three joining conditions with different heat input. Thorough analytical techniques were used to understand the physics of the process and the effect of the energy input on the final microstructure of the joined parts, the physico-chemical changes in the composite and the local and global mechanical properties of the joints. The process temperature reached values up to 761 ± 2°C indicating intrinsic degradation of the composite, formation of a softened/molten glass interlayer between the rivet and the composite and complex metallurgical transformations in the metallic rivet. Through monitoring of the process temperature and torque, an unstable friction regime was observed for FricRiveting of pultruded thermoset composites leading to distinguished extents of composite degradation. The microstructure of the Ti-6Al-4V alloy changed across the length of the rivet, from the equiaxed morphology to acicular structures in the rivet tip, where plastic deformation occurred. Three microstructural zones were proposed for each joint part including two thermo-mechanically affected zones and a heat affected zone. Microhardness mapping was performed in the metallic rivet evidencing an increase from the center to the tip of the rivet, with a hardness increment of over 20% compared to the base material (HVTi6Al4V= 300- 320 HV). The glass interphase consolidated in the metallic surface reached values of up to 974 HV followed by a drastic decrease to 24 HV in the polyester matrix located out of the joint area. The ultimate bearing strength ranged between 60 MPa and 166 MPa. Lesser composite degraded areas led to stronger joints. Two failure modes were observed combining initial composite bearing followed by final failure through shear of the rivet with partial rivet pullout or by full rivet pull-out. Complex failure micro-mechanisms were observed including the combination of cohesive and adhesive failures through the glass layer and the damaged composite interface. Friction-riveted joints achieved an ultimate lap shear strength of up to 80% to that of a similar bolted joint. A case study for a presumptive truss bridge application of friction-riveted joints showed a necessary of 92 rivets per truss node, 43% less than previous studies and with potential for further optimization. / Rebitagem por Fricção é uma tecnologia de união pontual inovadora para estruturas híbridas metal-polímero. Esta tese de mestrado apresenta pela primeira vez na literatura um entendimento aprofundado do processo de Rebitagem por Fricção para juntas de metal e compósito termofixo. Juntas de rebite de Ti-6Al-4V e componente pultrudado de poliéster termofixo reforçado com fibra de vidro foram produzidas seguindo três condições de processo com diferentes aportes térmicos. Diversas técnicas analíticas foram utilizadas para entender a física do processo e o efeito do aporte térmico na microestrutura final dos componentes unidos, mudanças físico-químicas no compósito, formação de camada vítrea intermediária entre o compósito e o rebite metálico e transformações metalúrgicas. Através do monitoramento da temperatura processual e do torque, um regime friccional não estável foi observado para a Rebitagem por Fricção de compósito pultrudado termofixo resultando em distintas extensões da degradação do compósito. A microestrutura da liga de Ti-6Al-4V transforma-se ao longo da secção transversal do rebite, de uma morfologia equiaxial no centro do rebite para uma estrutura acicular em sua extremidade, onde ocorre deformação plástica. Três zonas microestruturais foram propostas para cada componente da junta incluindo duas zonas termo mecanicamente afetadas e uma termicamente afetada. Mapa de microdureza foi realizado no rebite metálico evidenciando um aumento do centro para a extremidade do mesmo, próximo a 20% comparado com o material de base (HVTi6Al4V= 300-320 HV). A interfase vítrea consolidada na superfície do rebite metálico apresentou dureza em torno de 974 HV seguido de um drástico decaimento para 24 HV na matriz de poliéster localizada fora da região de união. A tensão máxima de apoio variou entre 60 e 166 MPa. Juntas com menor área degradada apresentaram os melhores desempenhos mecânicos em ensaio quase estático de cisalhamento. Dois modos de falha foram observados combinando um modo de falha inicial por deformação plástica severa no compósito seguida de falha final por cisalhamento no rebite metálico com parcial remoção do mesmo ou por remoção completa do rebite. Complexos micro mecanismos de falha foram observados incluindo a combinação de falha adesiva e coesiva através da interface entre interfase vítrea e compósito degradado. Juntas rebitadas por fricção atingiram resistência ao cisalhamento de 80% da obtida para juntas parafusadas. O estudo de caso para uma ponte hipotética treliçada revelou um número necessário de rebites de até 92 rebites por nó da ponte, 43% a menos que o encontrado em trabalhos anteriores, com potencial para futuras optimizações.

[ES] Las arritmias cardíacas son un problema importante para los sistemas de salud en el mundo desarrollado debido a su alta incidencia y prevalencia a medida que la población envejece. La fibrilación auricular (FA) y la fibrilación ventricular (FV) se encuentran entre las arritmias más complejas observadas en la práctica clínica. Las consecuencias clínicas de tales alteraciones arrítmicas incluyen el desarrollo de eventos cardioembólicos complejos en la FA, y repercusiones dramáticas debido a procesos fibrilatorios sostenidos que amenazan la vida infringiendo daño neurológico tras paro cardíaco por FV, y que pueden provocar la muerte súbita cardíaca (MSC). Sin embargo, a pesar de los avances tecnológicos de las últimas décadas, sus mecanismos intrínsecos se comprenden de forma incompleta y, hasta la fecha, las estrategias terapéuticas carecen de una base mecanicista suficiente y poseen bajas tasas de éxito. Entre los mecanismos implicados en la inducción y perpetuación de arritmias cardíacas, como la FA, se cree que las dinámicas de las fuentes focales y reentrantes de alta frecuencia, en sus diferentes modalidades, son las fuentes primarias que mantienen la arritmia. Sin embargo, se sabe poco sobre los atractores, así como, de la dinámica espacio-temporal de tales fuentes fibrilatorias primarias, específicamente, las fuentes focales o rotacionales dominantes que mantienen la arritmia. Por ello, se ha desarrollado una plataforma computacional, para comprender los factores (activos, pasivos y estructurales) determinantes, y moduladores de dicha dinámica. Esto ha permitido establecer un marco para comprender la compleja dinámica de los rotores con énfasis en sus propiedades deterministas para desarrollar herramientas basadas en los mecanismos para ayuda diagnóstica y terapéutica. Comprender los procesos fibrilatorios es clave para desarrollar marcadores y herramientas fisiológica- y clínicamente relevantes para la ayuda de diagnóstico temprano. Específicamente, las propiedades espectrales y de tiempo-frecuencia de los procesos fibrilatorios han demostrado resaltar el comportamiento determinista principal de los mecanismos intrínsecos subyacentes a las arritmias y el impacto de tales eventos arrítmicos. Esto es especialmente relevante para determinar el pronóstico temprano de los supervivientes comatosos después de un paro cardíaco debido a fibrilación ventricular (FV). Las técnicas de mapeo electrofisiológico, el mapeo eléctrico y óptico cardíaco, han demostrado ser recursos muy valiosos para dar forma a nuevas hipótesis y desarrollar nuevos enfoques mecanicistas y estrategias terapéuticas mejoradas. Esta tecnología permite además el trabajo multidisciplinar entre clínicos y bioingenieros, para el desarrollo y validación de dispositivos y metodologías para identificar biomarcadores multi-dominio que permitan rastrear con precisión la dinámica de las arritmias identificando fuentes dominantes y atractores con alta precisión para ser dianas de estrategias terapeúticas innovadoras. Es por ello que uno de los objetivos fundamentales ha sido la implantación y validación de nuevos sistemas de mapeo en distintas configuraciones que sirvan de plataforma de desarrollo de nuevas estrategias terapeúticas. Aunque el mapeo panorámico es el método principal y más completo para rastrear simultáneamente biomarcadores electrofisiológicos, su adopción por la comunidad científica es limitada principalmente debido al coste elevado de la tecnología. Aprovechando los avances tecnológicos recientes, nos hemos enfocado en desarrollar, y validar, sistemas de mapeo óptico de alta resolución para registro panorámico cardíaco, utilizando modelos clínicamente relevantes para la investigación básica y la bioingeniería. / [CAT] Les arítmies cardíaques són un problema important per als sistemes de salut del món desenvolupat a causa de la seva alta incidència i prevalença a mesura que la població envelleix. La fibril·lació auricular (FA) i la fibril·lació ventricular (FV), es troben entre les arítmies més complexes observades a la pràctica clínica. Les conseqüències clíniques d'aquests trastorns arítmics inclouen el desenvolupament d'esdeveniments cardioembòlics complexos en FA i repercussions dramàtiques a causa de processos fibril·latoris sostinguts que posen en perill la vida amb danys neurològics posteriors a la FV, que condueixen a una aturada cardíaca i a la mort cardíaca sobtada (SCD). Tanmateix, malgrat els avanços tecnològics de les darreres dècades, els seus mecanismes intrínsecs s'entenen de forma incompleta i, fins a la data, les estratègies terapèutiques no tenen una base mecanicista suficient i tenen baixes taxes d'èxit. La majoria dels avenços en el desenvolupament de biomarcadors òptims i noves estratègies terapèutiques en aquest camp provenen de tècniques valuoses en la investigació de mecanismes d'arítmia. Entre els mecanismes implicats en la inducció i perpetuació de les arítmies cardíaques, es creu que les fonts primàries subjacents a l'arítmia són les fonts focals reingressants d'alta freqüència dinàmica i AF, en les seves diferents modalitats. Tot i això, se sap poc sobre els atractors i la dinàmica espaciotemporal d'aquestes fonts primàries fibril·ladores, específicament les fonts rotacionals o focals dominants que mantenen l'arítmia. Per tant, s'ha desenvolupat una plataforma computacional per entendre determinants actius, passius, estructurals i moduladors d'aquestes dinàmiques. Això va permetre establir un marc per entendre la complexa dinàmica multidomini dels rotors amb ènfasi en les seves propietats deterministes per desenvolupar enfocaments mecanicistes per a l'ajuda i la teràpia diagnòstiques. La comprensió dels processos fibril·latoris és clau per desenvolupar puntuacions i eines rellevants fisiològicament i clínicament per ajudar al diagnòstic precoç. Concretament, les propietats espectrals i de temps-freqüència dels processos fibril·latoris han demostrat destacar un comportament determinista important dels mecanismes intrínsecs subjacents a les arítmies i l'impacte d'aquests esdeveniments arítmics. Mitjançant coneixements previs, processament de senyals, tècniques d'aprenentatge automàtic i anàlisi de dades, es va desenvolupar una puntuació de risc mecanicista a la aturada cardíaca per FV. Les tècniques de cartografia òptica cardíaca i electrofisiològica han demostrat ser recursos inestimables per donar forma a noves hipòtesis i desenvolupar nous enfocaments mecanicistes i estratègies terapèutiques. Aquesta tecnologia ha permès durant molts anys provar noves estratègies terapèutiques farmacològiques o ablatives i desenvolupar mètodes multidominis per fer un seguiment precís de la dinàmica d'arrímies que identifica fonts i atractors dominants. Tot i que el mapatge panoràmic és el mètode principal per al seguiment simultani de paràmetres electrofisiològics, la seva adopció per part de la comunitat multidisciplinària d'investigació cardiovascular està limitada principalment pel cost de la tecnologia. Aprofitant els avenços tecnològics recents, ens centrem en el desenvolupament i la validació de sistemes de mapes òptics de baix cost per a imatges panoràmiques mitjançant models clínicament rellevants per a la investigació bàsica i la bioenginyeria. / [EN] Cardiac arrhythmias are a major problem for health systems in the developed world due to their high incidence and prevalence as the population ages. Atrial fibrillation (AF) and ventricular fibrillation (VF), are amongst the most complex arrhythmias seen in the clinical practice. Clinical consequences of such arrhythmic disturbances include developing complex cardio-embolic events in AF, and dramatic repercussions due to sustained life-threatening fibrillatory processes with subsequent neurological damage under VF, leading to cardiac arrest and sudden cardiac death (SCD). However, despite the technological advances in the last decades, their intrinsic mechanisms are incompletely understood, and, to date, therapeutic strategies lack of sufficient mechanistic basis and have low success rates. Most of the progress for developing optimal biomarkers and novel therapeutic strategies in this field has come from valuable techniques in the research of arrhythmia mechanisms. Amongst the mechanisms involved in the induction and perpetuation of cardiac arrhythmias such AF, dynamic high-frequency re-entrant and focal sources, in its different modalities, are thought to be the primary sources underlying the arrhythmia. However, little is known about the attractors and spatiotemporal dynamics of such fibrillatory primary sources, specifically dominant rotational or focal sources maintaining the arrhythmia. Therefore, a computational platform for understanding active, passive and structural determinants, and modulators of such dynamics was developed. This allowed stablishing a framework for understanding the complex multidomain dynamics of rotors with enphasis in their deterministic properties to develop mechanistic approaches for diagnostic aid and therapy. Understanding fibrillatory processes is key to develop physiologically and clinically relevant scores and tools for early diagnostic aid. Specifically, spectral and time-frequency properties of fibrillatory processes have shown to highlight major deterministic behaviour of intrinsic mechanisms underlying the arrhythmias and the impact of such arrhythmic events. Using prior knowledge, signal processing, machine learning techniques and data analytics, we aimed at developing a reliable mechanistic risk-score for comatose survivors of cardiac arrest due to VF. Cardiac optical mapping and electrophysiological mapping techniques have shown to be unvaluable resources to shape new hypotheses and develop novel mechanistic approaches and therapeutic strategies. This technology has allowed for many years testing new pharmacological or ablative therapeutic strategies, and developing multidomain methods to accurately track arrhymia dynamics identigying dominant sources and attractors. Even though, panoramic mapping is the primary method for simultaneously tracking electrophysiological parameters, its adoption by the multidisciplinary cardiovascular research community is limited mainly due to the cost of the technology. Taking advantage of recent technological advances, we focus on developing and validating low-cost optical mapping systems for panoramic imaging using clinically relevant models for basic research and bioengineering. / Calvo Saiz, CJ. (2022). Novel Cardiac Mapping Approaches and Multimodal Techniques to Unravel Multidomain Dynamics of Complex Arrhythmias Towards a Framework for Translational Mechanistic-Based Therapeutic Strategies [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/182329 / TESIS

The aim of this work is informed first about photovoltaics universally, works to inform the photovoltaic panels and complete plants. The work also includes instructions on how to implement PVP in accordance with law. Another part is the rough draft of the photovoltaic power 30 kWp, which can be placed on the house, computation and calculation of investment and them profitable investments to time. Design is made in two separate forms of the Fronius Solar and Sunny Design, their outputs are compared. The practical part of this work cooperates with the company SOLARTEC Ltd. for experimental measurements of the photovoltaic system and develop a methodology for setting the properties of real solar systems in operation from the measured data then stored in a database. These data further evaluate and compare the similar operating conditions. This data will show as the course of production of electricity during the typical day in percentage terms, depending on the incident irradiance, cell temperature, angle of incident radiation, etc. We can compare what it looks like an ideal day in terms of production of photovoltaic power, with the other days. Further are in work mentioned histograms achievement panel behind classical day and behind all - time investigation.

Submitted by Luciclea Silva (luci@ufpa.br) on 2018-07-19T17:42:08Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ avaliacaodesempenhofuncoes.pdf: 5239412 bytes, checksum: a7be25c49d350063c87bb85986bb7199 (MD5) / Approved for entry into archive by Luciclea Silva (luci@ufpa.br) on 2018-07-19T17:42:46Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ avaliacaodesempenhofuncoes.pdf: 5239412 bytes, checksum: a7be25c49d350063c87bb85986bb7199 (MD5) / Made available in DSpace on 2018-07-19T17:42:46Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ avaliacaodesempenhofuncoes.pdf: 5239412 bytes, checksum: a7be25c49d350063c87bb85986bb7199 (MD5) Previous issue date: 2018-03-27 / Esta dissertação aborda alguns dos principais problemas operacionais enfrentados pelos operadores de redes de distribuição devido à elevada integração de geradores fotovoltaicos (FV), tais como sobretensões e comutações excessivas de tape nos autotransformadores reguladores de tensão. Essas situações estão sendo continuamente estudadas, tendo em vista que podem causar problemas de qualidade de tensão e reparos em reguladores de tensão. O problema da sobretensão pode ser causado por uma injeção significativa de potência ativa da geração FV à rede. Paralelamente, as variações rápidas e significativas na potência ativa injetada pelo gerador FV, associadas com os transitórios de nuvens, podem resultar no aumento excessivo de comutações de tape no regulador de tensão, que podem acabar diminuindo a vida útil e aumentar a quantidade de manutenções realizadas nestes equipamentos. Neste contexto, surgiram as funções de controle Volt-Watt e Volt-Var prensentes nos inversores FV inteligentes, que foram adicionadas em estudos recentes para mitigar as sobretensões. Este trabalho mostra como as funções de controle Volt-Watt e Volt-Var mitigam os problemas de sobretensão e regulação de tensão em redes de distribuição com presença de geradores FV. Simulações de fluxo de potência de séries temporais em alimentadores simples de BT e MT com presença da geração FV foram realizadas em cenários de alta e baixa variabilidade solar. / This dissertation addresses some of the major operational problems faced by distribution system operators due to the high integration of photovoltaic (PV) generators, such as overvoltages and excessive tap counts in step voltage regulators (SVR). These situations are continually being studied, since they can cause voltage quality problems and damage in SVRs. The overvoltage problem can be caused by a significant injection of active power from the PV generation to the network. Moreover, the rapid and significant variations in PV generator active power associated with cloud transients result in an increase of voltage regulator tap counts, which can affect the useful life of device. In this context, the smart inverters Volt-Watt and Volt-Var control functions have been added in recent studies to mitigate the overvoltages. This work shows how Volt-Watt and Volt-Var control functions mitigate the overvoltage and voltage regulation problems in distribution networks with PV generation. Time series power flow simulations on LV and MV single feeders with PV solar plant have been performed in high and low solar variability scenarios.

Abstract: Neurodegenerative diseases are associated with progressive loss of structure or function of neurons which results in cell death. Recent evidence indicate that all neurodegenerative disorders, sporadic or transmissible, may have a common pathological mechanism at the molecular level. This common feature consists of protein aggregation and accumulation of harmful aggregates in neuronal cells resulting in cellular apoptosis and neurotoxicity. Neurodegenerative diseases can affect abstract thinking, skilled movements, emotional feelings, cognition, memory and other abilities. This diverse group of diseases includes Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), prion diseases or transmissible spongiform encephalopathies (TSEs) and amyotrophic lateral sclerosis. In my project I worked on the molecular mechanism of protein aggregation, propagation and neurotoxicity in Parkinson's disease and prion disease. Prion disease and PD are associated with misfolding and aggregation of PrPc and a-Synuclein (a-Syn), respectively. Despite being two important neurodegenerative disorders, molecular mechanisms of a-Syn or PrPC aggregation and amyloidogenesis are still unclear in PD and prion disease. Furthermore, the toxic protein species in PD have not been characterized yet. In this study we characterize the mechanism of a-Syn and PrPc misfolding in a physiological-like cell free condition in the absence of a-Syn aggregates, PrPc ggregated isoform (Pre's), denaturants or acidic environment. A number of studies indicate that dimerization of PrPc or a-Syn may be a key step in the aggregation process. To test this hypothesis we verified if enforced dimerization of PrPc or a-Syn may induce a conformational change reminiscent of the conversion of PrPc or a-Syn to PrPR' or a-Syn aggregates, respectively. We used a well-described inducible dimerization strategy where a dimerizing domain called FK506-binding protein (Fv) was fused to PrPc or a-Syn in order to produce chimeric proteins Fv-PrP and a-SynF'''. A divalent ligand AP20187 was used to induce protein dimerization. Addition of AP20187 to recombinant Fv-PrP in physiological-like conditions resulted in a rapid conformational change characterized by an increase in beta-sheet (13-Sheet) structure and simultaneous aggregation of the proteins. However, non-dimerized PrP formed 13-Sheet conformation in very slower rates. In the presence of AP20187, we also report a rapid random coil into 13-sheet conformational transformation of a-SynF" within 24 h, whereas wild type a-Syn showed 24 h delay to achieve P-sheet structure after 48 h. Electron microscopy experiments demonstrated that dimerization induced amyloid fibril formation after 48 h for both Fv-PrP and a-Syr?", whereas in the absence of dimerizing ligand AP20187, PrP or a-Syn converted into amyloid fibrils after 3 days or even later. Dimerization-induced Fv-PrP aggregates were partially resistant to PK digestion which is a characteristics of the naturally occurring PrPR'. The rates of amyloidogenesis in the presence of dimerization was also characterized by Thioflavin T (ThT) fluorescence probing. Whereas the stable structure of Fv-PrP showed no ThT binding for over 60 h of incubation at 37°C, the addition of AP20187 to Fv-PrP resulted in a time-dependent increase in ThT binding. As for a-SynR, dimerization accelerated the rate of ThT binding and amyloid formation comparing to the slower amyloidogenesis rate of wild type a-Syn in the absence of dimerizer AP20187. The impact of dimerization on a-Syn aggregation was further determined by Fluorescence ANS probing, indicating a higher affinity of dimerization-induced a-SynF" aggregates for binding to ANS comparing to wild type a-Syn aggregates. These results indicate that dimerization increases the aggregation and amyloidogenesis processes for Fv-PrP and a-SynF". Both Fv-PrP and a-SynF" amyloids were successfully propagated in vitro by protein misfolding amplification (PMCA) cycle. These results ar in agreement with the theory that all protein aggregates in neurodegenerative diseases propagate with the same molecular mechanism. Neurotoxicity of recombinant Fv-PrP and a-SynF" aggregates was determined in cellulo and in vivo, respectively. Aggregates of Fv-PrP were toxic to cultured cells whilst soluble Fv-PrP and amyloid fibres were harmless to the cells. When injected to the mice brain, both a-Syni" and a-Syn pre-fibrillar aggregates internalized cells and induced neurotoxicity in the hippocampus of wild-type mice. These recombinant toxic aggregates further converted into non-toxic amyloids which were successfully amplified by PMCA method, providing the first evidence for the in vitro propagation of synthetic a-Syn aggregates. These results suggest an important role for protein dimerization in aggregation and amyloidogenesis, and therefore, in the pathology of PD and prion disease. The similarities between aggregation, amyloidogenesis and toxicity of PrPC and ct-Syn provide further evidence on the existance of a prion-like mechanism in all neurodegenerative disorders. // Résumé: Les maladies neurodégénératives sont associées à la perte progressive des propriétés structurales ou fonctionnelles des neurones, ce qui engendre la mort des cellules. De récentes études indiquent que tous les désordres neurodégénératifs, sporadiques ou transmissibles, peuvent avoir un mécanisme pathologique commun au niveau moléculaire. Ce dispositif commun se compose de l'agrégation de protéines, de la propagation des agrégats, et de l'accumulation d’agrégats toxiques dans les cellules neuronales, menant à l'apoptose et à la neurotoxicité cellulaire. Les maladies neurodégénératives peuvent affecter la pensée abstraite, les mouvements habiles, les sentiments émotifs, la connaissance, la Mémoire et d'autres capacités cognitives. Ce groupe divers de maladies inclut la maladie d'Alzheimer (AD), de Parkinson (PD), de Huntington (HD), les maladies à prions ou encéphalopathies spongiformes transmissibles (TSEs) et la sclérose latérale amyotrophique (ALS). [symboles non conformes]