Indiana University-Purdue University Indianapolis (IUPUI) / Fetal alcohol spectrum disorder is a disease caused by prenatal alcohol exposure. It is characterized by craniofacial abnormalities, growth retardation, central nervous system defects, learning disabilities and a variety of other minor defects. Even though it affects 2-5% of individuals born every year, very little is known about the mechanisms that cause it. The zebrafish (Danio rerio) presents as an interesting and efficient model for studying this disease. This study provides some insight into the mechanisms underlying observed FASD phenotypes and, more specifically, the transcription factor elf3, which is downregulated in response to ethanol exposure during early embryonic development. Here we show a number of elf3 target genes that are downregulated during early development in response to ethanol exposure. We also give some insight into the expression pattern of elf3 in relation to zygotic genome activation. Translation blocking morpholino oligonucleotides were used to implicate Elf3 in epiboly movements during gastrulation and zebrafish tail development. Taken together these results help to strengthen the zebrafish as a model for FASD in addition to giving greater insight into both the expression pattern and role of Elf3 during development.

No description available.

The main objective of this study is to construct a profile on the child with Fetal alcohol syndrome that can assist individuals working with these children. The focus of the study is the recognition of the emotional needs of the child with Fetal alcohol syndrome. Most studies done previously suggest that children with Fetal alcohol syndrome show behaviour similar to children with Attention Deficit Hyperactive Disorder. Although a child with Fetal alcohol syndrome shows the same characteristics as a child with Attention Deficit Hyperactive Disorder, the manifestation of their emotional needs differ. The Child with Attention Deficit Hyperactive Disorder does not necessarily show symptoms of cognitive developmental delay where the child with Fetal alcohol syndrome show symptoms of cognitive developmental delays. A Gestalt play therapy model has been used to show that through play therapy the child with Fetal alcohol syndrome can be guided to emotional awareness. These techniques are easy to use and applicable in class situations where the childcare worker, teachers or counselor work with the child with Fetal alcohol syndrome. The empirical research was done by means of quantitative research with was done by using the Conner symptom checklist to determine if the child with Fetal alcohol syndrome is hyperactive and impulsive and qualitative research with was done by means of participating observation Gestalt play therapy with the child with Fetal alcohol syndrome. The results show that the child with Fetal alcohol syndrome is hyperactive, impulsive and inattentional. These guidelines provided in the study will help the teacher and the child with Fetal alcohol syndrome cope better in the classroom environment and the child will learn how to cope with his emotional behaviours. / Social Work / M. Diac. (Play Therapy)

The main objective of this study is to construct a profile on the child with Fetal alcohol syndrome that can assist individuals working with these children. The focus of the study is the recognition of the emotional needs of the child with Fetal alcohol syndrome. Most studies done previously suggest that children with Fetal alcohol syndrome show behaviour similar to children with Attention Deficit Hyperactive Disorder. Although a child with Fetal alcohol syndrome shows the same characteristics as a child with Attention Deficit Hyperactive Disorder, the manifestation of their emotional needs differ. The Child with Attention Deficit Hyperactive Disorder does not necessarily show symptoms of cognitive developmental delay where the child with Fetal alcohol syndrome show symptoms of cognitive developmental delays. A Gestalt play therapy model has been used to show that through play therapy the child with Fetal alcohol syndrome can be guided to emotional awareness. These techniques are easy to use and applicable in class situations where the childcare worker, teachers or counselor work with the child with Fetal alcohol syndrome. The empirical research was done by means of quantitative research with was done by using the Conner symptom checklist to determine if the child with Fetal alcohol syndrome is hyperactive and impulsive and qualitative research with was done by means of participating observation Gestalt play therapy with the child with Fetal alcohol syndrome. The results show that the child with Fetal alcohol syndrome is hyperactive, impulsive and inattentional. These guidelines provided in the study will help the teacher and the child with Fetal alcohol syndrome cope better in the classroom environment and the child will learn how to cope with his emotional behaviours. / Social Work / M. Diac. (Play Therapy)

Text in Afrikaans with a summary in Afrikaans and English / The motivation for this study was to use animal-assisted therapy to the advantage of the child with Fetal Alcohol Syndrome. The purpose of this study was to describe the use of therapeutic horse riding in Gestalt therapy and its influence on the awareness levels of the child with Fetal Alcohol Syndrome that present with Attention deficit/Hyperactivity disorder. For the purpose of this study a quantitative research approach with a descriptive nature was used. Semi-structured interviews were used to compile information about a single child in a therapeutic milieu. This information was combined with information gathered from the literature to compile guidelines for the counsellor. In this study horse riding was effectively combined with Gestalt play therapy to enhance the awareness levels of the child with Fetal Alcohol Syndrome. / Die motivering van hierdie ondersoek was om diergefasiliteerde terapie tot voordeel van die kind met Fetale Alkohol Sindroom te benut. Die doel van die studie was om die benutting van terapeutiese perdry in Gestaltspelterapie en die invloed daarvan op die bewustheidsvlakke van die kind met Fetale Alkohol Sindroom wat met Aandagafleibaarheid/hiperaktiwiteitsindroom presenteer te beskryf. Vir die doel van hierdie studie is kwalitatiewe navorsing met 'n beskrywende aard benut. Semi-gestruktureerde onderhoude is gebruik om inligting in te samel rakende 'n enkele kind in 'n terapeutiese milieu. Hierdie inligting is gekombineer met die inligting uit die literatuur ten einde riglyne vir die berader op te stel. In hierdie studie is perdry effektief by Gestaltspelterapie geintegreer om die kind met Fetale Alkohol Sindroom se bewustheidsvlakke te verhoog. / Practical Theology / M.Diac. (Play Therapy)

Text in Afrikaans with a summary in Afrikaans and English / The motivation for this study was to use animal-assisted therapy to the advantage of the child with Fetal Alcohol Syndrome. The purpose of this study was to describe the use of therapeutic horse riding in Gestalt therapy and its influence on the awareness levels of the child with Fetal Alcohol Syndrome that present with Attention deficit/Hyperactivity disorder. For the purpose of this study a quantitative research approach with a descriptive nature was used. Semi-structured interviews were used to compile information about a single child in a therapeutic milieu. This information was combined with information gathered from the literature to compile guidelines for the counsellor. In this study horse riding was effectively combined with Gestalt play therapy to enhance the awareness levels of the child with Fetal Alcohol Syndrome. / Die motivering van hierdie ondersoek was om diergefasiliteerde terapie tot voordeel van die kind met Fetale Alkohol Sindroom te benut. Die doel van die studie was om die benutting van terapeutiese perdry in Gestaltspelterapie en die invloed daarvan op die bewustheidsvlakke van die kind met Fetale Alkohol Sindroom wat met Aandagafleibaarheid/hiperaktiwiteitsindroom presenteer te beskryf. Vir die doel van hierdie studie is kwalitatiewe navorsing met 'n beskrywende aard benut. Semi-gestruktureerde onderhoude is gebruik om inligting in te samel rakende 'n enkele kind in 'n terapeutiese milieu. Hierdie inligting is gekombineer met die inligting uit die literatuur ten einde riglyne vir die berader op te stel. In hierdie studie is perdry effektief by Gestaltspelterapie geintegreer om die kind met Fetale Alkohol Sindroom se bewustheidsvlakke te verhoog. / Philosophy, Practical and Systematic Theology / M.Diac. (Play Therapy)

Thesis (MEdPsych (Educational Psychology)--University of Stellenbosch, 2007. / Fetal Alcohol Syndrome (FAS) is an ongoing problem in the Western Cape. Marginalised and poverty-stricken communities use alcohol as a method of entertainment because it is freely available and relatively inexpensive. Due to a cycle of ongoing poverty and lack of education, many women drink large quantities of alcohol when they are pregnant or before they know they are pregnant. This causes the unborn baby to be severely at risk for FAS. There has been much research done in academic and social environments on the presentation and symptoms of FAS and of behaviour. Less research has been done surrounding the mother's experience of her FAS child, it is therefore my aim to research this gap in the research. This research study investigated the experience of mothers who raised children with FAS. Many mothers of children with prenatal exposure to alcohol feel conflict and guilt regarding their children and I attempted to find out what their general experience surrounding this was. Within this research topic I aimed to investigate the mothers' attitudes, their behaviour towards and their general perceptions of their developing child with FAS. This was viewed from an eco-systemic framework in which the mother is an integral part of different systems impacting and working together, that influence her maternal functioning. Finally, the aim of this research study was to ascertain how best mothers of FAS children could be supported. In this same process, I hoped, the mothers could learn to feel empowered to help and support their child, and in the process attempt to shift ongoing cycles of negative behaviour patterns to more positive outcomes.

Introdução: O consumo de bebidas alcoólicas por gestantes acarreta alterações de formação e de desenvolvimento fetal, incluindo alterações estruturais e funcionais do Sistema Nervoso Central. Os comprometimentos sensoriais de crianças expostas ao álcool na gestação, como a audição, são uma das morbidades relatadas nesta população. A ocorrência de alteração da sensitividade auditiva é melhor conhecida, mas o envolvimento do sistema nervosos auditivo central ainda carece de pesquisas. Objetivo: Estudar a função auditiva, periférica e central, em crianças em idade escolar com expostas ao álcool no período pré-natal. Métodos: Foram avaliadas 45 crianças, de ambos os gêneros, com 13 e 14 anos, provenientes do projeto INFANTO-ÁLCOOL, divididas em grupo exposto (GE) e não exposto (GNE) ao álcool na gestão. Foram realizadas audiometria tonal limiar, imitânciometria, emissões otoacústicas transientes (EOA-T), potencial evocado auditivo de tronco encefálico (PEATE) e o potencial evocado auditivo tardio, potencial evocado auditivo de longa latência (PEALL) e relacionado a eventos (PEARE), registrado em Cz e Pz. Foi realizada a análise comparativa entre grupos. Resultados: Apenas uma criança do GE apresentou perda auditiva unilateral do tipo mista, sendo os demais com limiares auditivos dentro da normalidade, com predominância de curva timpanomética do tipo \"A\" e presença de reflexo contralateral. Quanto ao funcionamento coclear e avaliação comportamental do processamento auditivo, não houve diferença entre grupos. Embora os resultados do PEATE tenham sido sugestivos de alteração na sincronia neural em nível de tronco encefálico, a diferença entre grupos não foi apontada. Para o PEALL, o GE apresentou menor amplitude de P2, na derivação Pz; no PEARE maiores amplitudes de P3a foram evidenciadas no GE, em Cz. Na comparação entre os eletrodos ativos foi possível constatar maior identificação da P3 com duplo pico para o eletrodo em Pz. Conclusão: O perfil de exposição fetal ao álcool em qualquer trimestre gestacional, esteve relacionado a alteração na sincronia neural em nível de tronco encefálico, bem como na identificação das componentes P2 e P3a. / Introduction: The consumption of alcohol by pregnant women causes abnormalities on fetal growth and development, including structural and functional changes in the Central Nervous System. The sensory impairments of children exposed to alcohol during pregnancy, such as hearing, are one of the morbidities reported in this population. The occurrence of change in hearing sensitivity is better known, but the auditory central nervous system involvement still needs more research. Objective: To study the hearing function, peripheral and central, in school-age children with exposed to alcohol prenatally. Methods: 45 children were assessed, of both genders, in age of 13 and 14 years, from INFANTO-ÁLCOOL project, allocated in two groups, exposed (EG) and not exposed (NEG) to alcohol during pregnancy. Following tests were performed: - pure tone audiometry, acoustic immittance, transient otoacoustic emissions (TOEA), brainstem auditory responses (ABR), late auditory evoked potentials, longlatency auditory evoked potential (LLAEP) and event-related (ERAP), registered in Cz and Pz, with comparative analysis between groups. Results: Only one child of EG had a unilateral mixed hearing loss, being the others participants found in the normal hearing thresholds, with predominance of timpanometric curve of type \"A\" and presence of contralateral acoustic reflex. Regarding the cochlear function and behavioral auditory processing assessment, there were no differences between groups. Although, the ABR results have been suggestive of change in neural synchrony brainstem level, the difference between groups was not confirmed. For the LLAEP, the EG showed lower amplitude of P2, in Pz deriving electrode; to the EARP P3a component had larger amplitudes in EG, in Cz. The comparison between the active electrode was possible to record a greater identification of the P3 components with double peak for the electrode in Pz. Conclusion: The profile of fetal exposure to alcohol in any trimester dates was related to change in neural synchrony in brainstem level as well as in the identification of P2 and P3a components.

Introdução: O consumo de bebidas alcoólicas por gestantes acarreta alterações de formação e de desenvolvimento fetal, incluindo alterações estruturais e funcionais do Sistema Nervoso Central. Os comprometimentos sensoriais de crianças expostas ao álcool na gestação, como a audição, são uma das morbidades relatadas nesta população. A ocorrência de alteração da sensitividade auditiva é melhor conhecida, mas o envolvimento do sistema nervosos auditivo central ainda carece de pesquisas. Objetivo: Estudar a função auditiva, periférica e central, em crianças em idade escolar com expostas ao álcool no período pré-natal. Métodos: Foram avaliadas 45 crianças, de ambos os gêneros, com 13 e 14 anos, provenientes do projeto INFANTO-ÁLCOOL, divididas em grupo exposto (GE) e não exposto (GNE) ao álcool na gestão. Foram realizadas audiometria tonal limiar, imitânciometria, emissões otoacústicas transientes (EOA-T), potencial evocado auditivo de tronco encefálico (PEATE) e o potencial evocado auditivo tardio, potencial evocado auditivo de longa latência (PEALL) e relacionado a eventos (PEARE), registrado em Cz e Pz. Foi realizada a análise comparativa entre grupos. Resultados: Apenas uma criança do GE apresentou perda auditiva unilateral do tipo mista, sendo os demais com limiares auditivos dentro da normalidade, com predominância de curva timpanomética do tipo \"A\" e presença de reflexo contralateral. Quanto ao funcionamento coclear e avaliação comportamental do processamento auditivo, não houve diferença entre grupos. Embora os resultados do PEATE tenham sido sugestivos de alteração na sincronia neural em nível de tronco encefálico, a diferença entre grupos não foi apontada. Para o PEALL, o GE apresentou menor amplitude de P2, na derivação Pz; no PEARE maiores amplitudes de P3a foram evidenciadas no GE, em Cz. Na comparação entre os eletrodos ativos foi possível constatar maior identificação da P3 com duplo pico para o eletrodo em Pz. Conclusão: O perfil de exposição fetal ao álcool em qualquer trimestre gestacional, esteve relacionado a alteração na sincronia neural em nível de tronco encefálico, bem como na identificação das componentes P2 e P3a. / Introduction: The consumption of alcohol by pregnant women causes abnormalities on fetal growth and development, including structural and functional changes in the Central Nervous System. The sensory impairments of children exposed to alcohol during pregnancy, such as hearing, are one of the morbidities reported in this population. The occurrence of change in hearing sensitivity is better known, but the auditory central nervous system involvement still needs more research. Objective: To study the hearing function, peripheral and central, in school-age children with exposed to alcohol prenatally. Methods: 45 children were assessed, of both genders, in age of 13 and 14 years, from INFANTO-ÁLCOOL project, allocated in two groups, exposed (EG) and not exposed (NEG) to alcohol during pregnancy. Following tests were performed: - pure tone audiometry, acoustic immittance, transient otoacoustic emissions (TOEA), brainstem auditory responses (ABR), late auditory evoked potentials, longlatency auditory evoked potential (LLAEP) and event-related (ERAP), registered in Cz and Pz, with comparative analysis between groups. Results: Only one child of EG had a unilateral mixed hearing loss, being the others participants found in the normal hearing thresholds, with predominance of timpanometric curve of type \"A\" and presence of contralateral acoustic reflex. Regarding the cochlear function and behavioral auditory processing assessment, there were no differences between groups. Although, the ABR results have been suggestive of change in neural synchrony brainstem level, the difference between groups was not confirmed. For the LLAEP, the EG showed lower amplitude of P2, in Pz deriving electrode; to the EARP P3a component had larger amplitudes in EG, in Cz. The comparison between the active electrode was possible to record a greater identification of the P3 components with double peak for the electrode in Pz. Conclusion: The profile of fetal exposure to alcohol in any trimester dates was related to change in neural synchrony in brainstem level as well as in the identification of P2 and P3a components.

The Ph.D. dissertation encompasses an interdisciplinary study exploring qualitative, holistic strategies for individuals with Fetal Alcohol Spectrum Disorder (FASD) in integrated areas of law, medicine, education, psychology and justice, through both inductive analysis of field research as well as through relevant documentary analysis, incorporating a global or comparative component. Compliance with Guidelines for Research Involving Aboriginal Peoples has been sustained through community partnerships with various First Nations and Métis Communities, Elders and Parents, as well as with an FASD Parental Advocacy Group, advised by a team of interdisciplinary researchers in the academy. Accordingly, emergent research protocols were co-constructed through ongoing collaboration with the various community partners. In Aboriginal research, it is essential not to parachute in and out of communities with the data, but rather to forge genuine, collaborative, long term partnerships, and to build capacity in those communities.<p> The dissertation format approved by the Student Advisory Committee is Manuscript Style, a format approved by the University of Saskatchewans College of Graduate Studies and Research (formerly referred to as X-Format) similar to a self-edited book or collection of articles with introduction, sub-text, intra-text and general discussion to link the manuscripts. The various manuscripts comprising the present thesis include:<p> 1.Framing the Research Anthology: A Vision Quest, Ékehohksimoht Ke-kiss-see Muya<p> Section One situates the research style, process, approach, substance and rationale of the dissertation. It is largely situated within holistic Indigenous epistemologies, which may require a paradigm shift, in contrast to more bounded western world views.<p> Interdisciplinary, holistic, community-based research on the topic of FASD, including a search for solutions, extends globally, across the lifespan, and across sectors.<p> II. Indigenous Disadvantage and Despair, An Evaluation of Recent Strategies and Alternatives: Healing and Transformation, Pluralism and Reconciliation, Ne wah kuma ka tik<p> Section Two explores historical and contextual factors leading to a high prevalence of FASD, as well as strategies to overcome disadvantage, including Reconciliation, Treaty Processes, and Research as Reconciliation. Local Narratives are privileged over Meta-narratives, to counter the power of global market forces usurping the sphere of family, community and culture.<p> III. Disjunctures and Discontinuities in the Law of Mental Intent: FASD as a Site of Resistance and Transformation, Esquiskuit<p> Section Three examines the disconnect between medical knowledge of FASD, on the one hand, and the Laws of Mental Intent, on the other, inspiring a search for a unified, integrated theory of mental disorder and criminal responsibility that takes into account modern neurocognitive conditions like FASD. Section Three further explores the present piecemeal and compartmentalized rules for fitness, responsibility, various levels of mental intent, and a resultant rationale, substance and process of law reform and systemic change.<p> IV. FASD and Holistic Literacies: A Talking or Sharing Circle, Wa-sa-cam-e-be-ke-skue<p> Section Fours inductive themes comprise model practice guidelines for the gestalt of Literacy and FASD, derived from inductive analysis of qualitative data collected in the field research. The data was collected using Sharing Circles with Aboriginal Elders, Parents, and Mentors of Individuals with FASD; Conversational Interviews with Parents and Children with FASD; as well as Interviews and Focus Groups with various Professionals who support individuals with FASD and their Families. Special protocols were followed in creating and participating in the Indigenous Research, Sharing Circles and Conversational Interviews. Meta-paradigmatic analysis situates Indigenous Research Methodologies among emerging, multi-disciplinary, inductive methodologies suitable for understanding the infinite complexity of natural phenomena, such as FASD.<p> V. Epilogue: An Honour Song, Kethou-ne-ka-mon<p> Circles of healing, transformation and reconciliation heal wounds, reconcile differences, and transform paradigms of justice, health, education and governance, through the incorporation of models of equitable, holistic relationships with one another and with Mother Earth. Multidisciplinary and cross-cultural perspectives, dialogues between local and global, and particular and universal, become matrices to support new paradigms embodying broader reflections of reality.