Plants secrete peptide ligands and use receptor signaling to respond to stress and control development. Understanding the signaling mechanisms and associated molecular trafficking is key to improving plant health and productivity for food, fiber and energy applications. However, one of the challenges to elucidating communication pathways in plants is to study the trafficking of molecules and signals iteratively and non-destructively. This study focuses on using fiber-optic fluorescence microscopy to image live plants iteratively and non-destructively after delivering both labeled and unlabeled phytosulfokine (PSK) into the plant. PSK is a sulfated peptide hormone involved in the regulation of plant cell division and growth via specific receptors, PSKRs. It also plays a role in regulating how plants are able to tolerate stress conditions. The microscope provides two-color (FITC/TRITC) optics and provides high-resolution (3–5 µm) epifluorescence micrographs via a 1-m coherent imaging fiber and a GRIN objective lens. To obtain high-quality images, the fiber was mounted either to a conventional upright microscope body equipped with a leaf compressor, or to a leaf clip with 5-axis positioning (X–Y–Z plus pitch and yaw) mounted on an extensible arm. PSK and TAMRA-labelled PSK were delivered into the roots of various Arabidopsis thaliana genotypes (wt; receptor-deficient: pskr1/pskr2; and tagged receptor overproducing: PSKR1‑GFP), and their movement in roots and leaves was tracked with the fiber-optic fluorescence microscope. Peptide trafficking was successfully observed in live plants non- destructively, confirming that PSK is mobile in both wt and receptor-deficient plants. Preliminary results suggest that the level of receptor PSKR1 may change in response to PSK, and that levels of PSKR1, PSKR2 or both may impact the trafficking of PSK. Understanding how PSK is trafficked in plants will offer insights into how we can improve plants health and productivity.

京都大学 / 0048 / 新制・課程博士 / 博士(情報学) / 甲第21216号 / 情博第669号 / 新制||情||115(附属図書館) / 京都大学大学院情報学研究科通信情報システム専攻 / (主査)教授 守倉 正博, 教授 大木 英司, 教授 梅野 健 / 学位規則第4条第1項該当 / Doctor of Informatics / Kyoto University / DFAM

Laser induced damage is of interest in studying the transmission of large amounts of optical energy through step-index, large core multimode fibers. Optical fibers often have to be routed around objects when laser light is being transmitted between two locations which require the fiber to bend into a curve. Depending on how tight the bend is, this can result in transmission losses or even catastrophic damage when the energy density of the laser pulse exceeds the damage threshold of silica glass. The purpose of this study is to: Establish a minimum bend radius that would allow high energy (GW/cm2 ) to be transmitted through multimode fiber. Evaluate unique fiber routing configurations including loops, 180 bends, and S-bends. Develop optical modeling simulations backed with experimental data that can serve to predict critical areas for future systems. Waveguide theory predicts that light traveling through a bend will form whispering-gallery modes that propagate through total internal reflection bounces along the inside of the outer edge of the bend. This is critical since in these locations the energy density of the light will increase significantly, raising the potential of laser damage, nonlinear effects, and transmission losses. This loss is especially problematic when two 90° bends going in opposite directions are in close proximity to each other, forming an ‘S-bend’. Light that is grouped along the outer edge going through the first bend will enter the second bend at a sharper angle which causes much high transmission losses and raises the possibility of failure. iv Models using R-Soft BeamProp and Zemax were developed to study transmission losses, investigate light interactions at critical areas, and predict under which conditions laser damage would occur. BeamProp presents a clearer view of the modal distribution of light within the core of the fiber and is used to analyze how a plane wave with a Gaussian intensity distribution excites the fiber modes. Zemax provides a tool to perform non-sequential ray tracing through the fiber cable and stray light analysis within the core and once the light exits the fiber. Intensity distributions of the cross sectional area of the fiber shows the whispering gallery modes forming as the light propagates around bends and disburses as it propagates afterwards. It was discovered using R-Soft that if the separation distance between bends in an S-bend is approximately 3 mm there exists a condition where maximum transmission occurs. For 365 µm diameter core fiber it was calculated that the difference in output power could be as high as 150%. This was initially completely unexpected; however ray tracing using Zemax was able to verify that this distance allows the light to transition so that it enters the 2nd bend at the optimal angle to enter the whispering gallery mode. Experiments were performed that validated the models’ predictions and images were captured clearly showing the spatial distribution shift of the light within the core of the fiber. Experiments were performed to verify light grouping together to form whispering gallery modes as predicted by Zemax. Microscope images were taken as a function of distance from various bends to observe the periodic nature in which the laser light fills up the fiber. Additionally, a configuration was setup to examine stimulated Brillioun scattering and determine the onset of laser damage in the fiber. Fibers were tested as a function of bend radius and number of shots v and recommendations for future systems were made. Lastly, mechanical failure tests were performed to determine the relationship between stress placed on the fiber through bending and fiber lifetime in a static environment. This allowed a minimum safe bend radius to be calculated for a 30 year lifetime that agreed with previous calculated values.

Durch die Kopplung von Szintillationskristallen an Lichtwellenleiter könnten robuste Messgeräte für die spektroskopitsche Messung von Strahlungsfeldgrößen in Bohrlöchern in Betonstrukturen beim Rückbau von Kernkraftwerken realisiert werden. In dieser Arbeit wurden zwei Prototypen solcher Messgeräte entwickelt, wobei ein auf einem Gadolinium-Aluminium-Gallium-Granat-Szintillationskristall basierendes Messsystem mit einem Kunststofflichtwellenleiter mit hohem Durchmesser aufgrund seiner hohen Zählrate bevorzugt wurde. Mit diesem Messystem konnten spektroskopische Messungen von Gammastrahlung durchgeführt werden. Weiterhin konnte gezeigt werden, dass Kontaminationen einer bestimmten Aktivität bis in einen Abstand von mehreren Zentimetern von der Messsonde im Beton nachweisbar sind. Ein Schätzwert der minimale Messzeit zur Erkennung einer nachweisbaren Kontamination konnte bestimmt werden. Eine hinreichend große, gemessene Ereigniszahl ermöglicht zusätzlich die Bestimmung des Abstands einer radioaktiven, punktförmigen Kontamination eines bekannten Nuklids. Für ein bekanntes Nuklid konnte weiterhin aus der Zählrate ein Schätzwert für die Energiedosis am Ort der Messsonde mithilfe von Referenzmessungen der Dosis mittels Berylliumoxid-Detektoren bestimmt werden.:Inhaltsverzeichnis Abbildungsverzeichnis vii Tabellenverzeichnis ix 1 Einleitung 1 2 Physikalische Grundlagen 3 2.1 Radioaktiver Zerfall 3 2.2 Gröÿen des Strahlungsfelds 3 2.3 Wechselwirkungen von Photonen mit Materie 5 2.4 Lichtentstehung in anorganischen Szintillatoren 8 2.5 Lichtmessung 10 2.6 Pulshöhenverteilungen von Szintillationsdetektoren 12 2.7 Ein ussfaktoren der Lichtausbeute 14 3 Material und Methoden 16 3.1 Aufbau LSO-Sonde und Messungen mit radioaktiven Quellen 16 3.2 Aufbau Lichtkopplung mittels Linse 18 3.3 Aufbau und Versuchsablauf Quarzglaskopplungen 20 3.4 Aufbau GAGG-Sonde 21 3.5 Aufbau des Betonphantoms 24 3.5.1 Dichtebestimmung des Betonphantoms 24 3.6 Messablauf Tiefenkurven in Betonphantom 27 3.7 Datenanalyse mittels Kolmogorv-Smirnov-Test 27 4 Ergebnisse 29 4.1 Spektroskopische Eigenschaften LSO-Sonde 29 4.2 Winkelverteilung austretender Photonen aus Szintillationskristall 33 4.3 Quarzglaskopplung 36 4.4 Spektroskopische Eigenschaften GAGG-Sonde 43 4.5 Vergleich GAGG- und LSO-Sonde 47 4.6 Abstandsabhängigkeit der Zählrate 50 4.7 Abschätzung der maximalen Abschirmdicke von Beton für Kontaminationserkennung 53 4.8 Abschätzung der minimalen Messzeit zur Kontaminationserkennung 56 4.9 Abschirmungsdickenbestimmung mittels Abschirmungsparameter 57 4.10 Bestimmung der Dosis 62 5 Diskussion 64 6 Zusammenfassung 67 / A robust measuring system for spectroscopic measurementes of gamma-ray radiation in boreholes in concrete structures can be built by coupling of a scintillation crystal to a light guide. Two prototypes of such measuring systems are developed one based on a Gadolinium-Aluminium-Gallium-Garnet scintillation crystal with a plastic optical fiber with a high diameter is preferred due to the higher count rate. Spectroscopic measurements of gamma-ray radiation with this measuring system can be performed. It can be shown that contamination of a specific activity can be detected even if they are located in concrete in a distance of several centimeters from the radiation sensor. for the minimal measurement time of 38 s An estimated value to detect a traceable contamination could be determined. If a high number of events can be detected, even the distance between a point like radioaktive source of a known nuclide and the radiation sensor can be determined. An estimated value for the applied dose at the place of the radiation sensor could be determined for a known nuclide with the help of the count rate. Therefore, reference measurements of dose with berylliumoxide detectors were performed.:Inhaltsverzeichnis Abbildungsverzeichnis vii Tabellenverzeichnis ix 1 Einleitung 1 2 Physikalische Grundlagen 3 2.1 Radioaktiver Zerfall 3 2.2 Gröÿen des Strahlungsfelds 3 2.3 Wechselwirkungen von Photonen mit Materie 5 2.4 Lichtentstehung in anorganischen Szintillatoren 8 2.5 Lichtmessung 10 2.6 Pulshöhenverteilungen von Szintillationsdetektoren 12 2.7 Ein ussfaktoren der Lichtausbeute 14 3 Material und Methoden 16 3.1 Aufbau LSO-Sonde und Messungen mit radioaktiven Quellen 16 3.2 Aufbau Lichtkopplung mittels Linse 18 3.3 Aufbau und Versuchsablauf Quarzglaskopplungen 20 3.4 Aufbau GAGG-Sonde 21 3.5 Aufbau des Betonphantoms 24 3.5.1 Dichtebestimmung des Betonphantoms 24 3.6 Messablauf Tiefenkurven in Betonphantom 27 3.7 Datenanalyse mittels Kolmogorv-Smirnov-Test 27 4 Ergebnisse 29 4.1 Spektroskopische Eigenschaften LSO-Sonde 29 4.2 Winkelverteilung austretender Photonen aus Szintillationskristall 33 4.3 Quarzglaskopplung 36 4.4 Spektroskopische Eigenschaften GAGG-Sonde 43 4.5 Vergleich GAGG- und LSO-Sonde 47 4.6 Abstandsabhängigkeit der Zählrate 50 4.7 Abschätzung der maximalen Abschirmdicke von Beton für Kontaminationserkennung 53 4.8 Abschätzung der minimalen Messzeit zur Kontaminationserkennung 56 4.9 Abschirmungsdickenbestimmung mittels Abschirmungsparameter 57 4.10 Bestimmung der Dosis 62 5 Diskussion 64 6 Zusammenfassung 67

Plants secrete peptide ligands and use receptor signaling to respond to stress and control development. Understanding these phenomena is key to improving plant health and productivity for food, fiber, and energy applications. Phytosulfokine (PSK), a sulfated peptide hormone, regulates plant cell division, growth, and stress tolerance via specific phytosulfokine receptors (PSKRs). This study uses fiber-optic fluorescence microscopy to elucidate trafficking of PSK in live plants. The microscope features two-color optics and an objective lens connected to a 1-m coherent imaging fiber mounted on either a conventional upright microscope body or 5-axis positioning system (X–Y–Z plus pitch and yaw). PSK and fluorescently-labelled PSK were delivered into roots and leaves of various Arabidopsis thaliana genotypes, and their movement was non-destructively tracked with the microscope. High-resolution (3–5 µm) epifluorescence micrographs confirmed that PSK is mobile in plants and levels of PSKR1, PSKR2, or both may impact the trafficking of PSK.

No description available.

No description available.

Beneath North America's roads lie 1.6 million miles of pipeline that provides users with potable water and carry away wastewater. These buried infrastructure systems have been functioning for duration longer than their intended design life, often with little or no repair. Asset management of pipeline systems pose a major challenge for most municipalities due to budgetary constraints, demand for quality service, and need to preserve existing pipeline infrastructure. The first step in developing and implementing a comprehensive asset management plan is to perform a condition assessment. There is a gamut of inspection and monitoring technologies available to enable the condition assessment of pipelines. All of these have advantages and limitations, which determine the performance quality and effectiveness of an individual technology for particular utility assets. Unfortunately, utilities choose technologies not suitable for their specific assets and collect data that is not useful for understanding the condition of their system. The objective of this thesis is to develop a framework for the effective selection of condition assessment technologies for water and wastewater utilities. A Microsoft-Excel based framework is developed to help the utility managers in selecting condition assessment technologies for their water and wastewater pipeline assets. The recommended tool selection approach uses a multi-step exclusion protocol in which the tools are excluded on the basis of their applicability relating to technical feasibility and technical suitability for a particular situation. Usable tools are then compared against a performance and cost database to determine performance and cost in a given project/ utility condition. This thesis provides a brief description and review of 24 non-destructive commercialized condition assessment technologies, including the principal and implementation considerations. A framework for decision system tool was developed to facilitate utilities in selecting appropriate condition assessment technologies. This framework could facilitate the selection of usable technologies by excluding the options which are not technically feasible and suitable. The user can then further explore the usable tools and determine the most suitable technologies for their assets. The data considered in the research is provided by technology providers, thus it may lack complete understanding of the capabilities and limitations of technology. This thesis also presents a case study which highlights the existing gap between the understanding of capabilities and limitations of various technologies. A program is developed as a part of this thesis, Condition Assessment Selection Tool (CAST), which consists of performance and economic database, a graphical user interface to facilitate user input, and the results of the comparison of each usable technology in the database to the project information provided by the user for their assets. The results are presented as performance indices and economic indices indicating the performance and technology cost of usable technologies. A data reliability index was also developed to provide a scale for comparing the reliability of the existing data in the database. / Master of Science

A fatigue test has been performed on 7075-T651 aluminum specimens which were bonded with polyimide coated optical fibers with discrete Bragg gratings. These fibers were bonded with AE-10 strain gage adhesive. The results indicate that lower strain amplitudes do not produce cause for concern, but that larger strain amplitudes (on the order of 3500 μ) may cause some sensors to become unreliable. The strain response of acrylate coated optical fiber strain sensors bonded to aluminum specimens with AE-10 and M-Bond 200 strain gage adhesives was investigated with both axial and cantilever beam tests. These results were compared to both the strain response of conventional strain gages and to model predictions. The results indicate that only about 82.6% of the strain in the specimen was transferred through the glue line and fiber coating into the fiber. Thus, multiplying by a strain transfer factor of approximately 1.21 was sufficient to correct the optical fiber strain output. This effect was found to be independent of the adhesive used and independent of the three-dimensional profile of the glue line used to attach the fiber. Finally, this effect did not depend on whether the fiber had a polyimide or an acrylate coating. Further investigation was conducted on the feasibility of using optical fiber strain sensors for monitoring subcritical damage (such as matrix cracks) in fiber reinforced composite materials. These results indicate that an array of optical fibers which monitor the strain profile on both sides of a composite panel may be sufficient for these purposes / Master of Science

[EN] Structural Health Monitoring (SHM) is a discipline that quantitatively assesses the integrity and performance of infrastructures, relying on sensors, and support the development of efficient Maintenance and Rehabilitation (M&R) plans. Optical Multicore Fiber (MCF) Shape Sensors offer an innovative alternative to traditional methods and enable the reconstruction of the deformed shape of structures directly and in real-time, with no need of computation models or visual contact and exploiting all the advantages of Optical Fiber Sensors (OFS) technology. Despite the intense research efforts centered on this topic by research groups worldwide, a comprehensive investigation on the parameters that influence the performance of these sensors has not been conducted yet. The first part of the thesis presents a numerical study that examines the effects of strain measurement accuracy and core position errors on the performance of optical multicore fiber shape sensors in sensing three-dimensional curvature, which is at the basis of shape reconstruction. The analysis reproduces the strain measurement process using Monte Carlo Method (MCM) and identifies several parameters which play a key role in the phenomenon, including core spacing (distance between outer cores and sensor axis), number of cores and curvature measured. Finally, a set of predictive models were calibrated, by fitting the results of the simulations, to predict the sensors performance. Afterward, an experimental study is proposed to evaluate the performance of optical multicore fiber in sensing shape, with particular focus on the influence of strain sensors length. Two shape sensors were fabricated, by inscribing long (8.0 mm) and short (1.5 mm) Fiber Bragg Gratings (FBG) into the cores of a multicore seven-core fiber. Thus, the performance of the two sensors was assessed and compared, at all the necessary phases for shape reconstruction: strain sensing, curvature calculation and shape reconstruction. To conclude, an innovative approach, based on the Saint-Venant's Torsion Theory, is presented to determine the twisting of multicore fiber and to compensate the errors due to twisting during shape reconstruction. The efficiency of the theoretical approach was then corroborated performing a series of twisting tests on a shape sensor, fabricated by inscribing FBGs sensors into an optical spun multicore seven-core fiber. The investigation of the mechanical behavior of multicore optical shape sensors has synergically involved diverse disciplines: Solid Mechanics, Photonics, Statistics and Data Analysis. Such multidisciplinary research has arisen from the prolific cooperation between the Institutes of the Institute of Science and Technology of Concrete (ICITECH) and the Institute of Telecommunications and Multimedia Applications (iTEAM) - Photonics Research Labs (PRL) - of Universitat Politècnica de València (UPV), in addition to valuable collaboration with other members of the European ITN-FINESSE project, to which this work belongs. This research work aims to enhance the performance optical multicore fiber shape sensors and support the development of new sensor geometries, with great potential for structural health monitoring applications. / [ES] La Monitorización de la Salud Estructural (MSE) evalúa cuantitativamente la integridad y el comportamiento de las infraestructuras y permite desarrollar planes eficaces de Mantenimiento y Rehabilitación (M&R), utilizando los datos de los sensores. Sensores de forma basados en fibra óptica multinúcleo ofrecen una alternativa a los métodos tradicionales y permiten la reconstrucción de la deformada de estructuras de forma directa y en tiempo real, sin necesidad de modelos de cálculo o contacto visual y con todas las ventajas de la tecnología de los Sensores de Fibra Óptica (SFO). A pesar de los grandes esfuerzos en la investigación centrada en este tema por parte de los grupos de investigación de todo el mundo, todavía no se ha realizado una investigación exhaustiva que estudie los parámetros que influyen en el comportamiento de estos sensores. En la primera parte de la tesis se presenta un estudio numérico en el que se examinan los efectos de la precisión de la medición de la tensión y los errores de posición del núcleo en el comportamiento de los sensores de forma basados en fibra óptica multinúcleo para definir la curvatura tridimensional, que es la base de la reconstrucción de la forma. El análisis reproduce el proceso de medición de la tensión utilizando el método de Monte Carlo (MC) e identifica una serie de parámetros que desempeñan un papel en el proceso, entre ellos la separación del núcleo (distancia entre los núcleos exteriores y el eje del sensor), el número de núcleos y la curvatura medida. Por último, se calibró un conjunto de modelos de predicción ajustando los resultados de las simulaciones para predecir el comportamiento de los sensores. A continuación, se propone un estudio experimental para evaluar el comportamiento de los sensores de forma basado en fibra óptica multinúcleo, con especial atención en la influencia de la longitud de los sensores de deformación. Se fabricaron dos sensores de forma, inscribiendo Fiber Bragg Gratings (FBG) con longitudes de 8,0 mm y 1,5 mm en los núcleos de una fibra multinúcleo de siete núcleos. Así, se evaluó y comparó el comportamiento de los dos sensores en todas las fases necesarias para la reconstrucción de la forma, incluyendo la medición de la tensión, el cálculo de la curvatura y la reconstrucción de la forma. Para concluir, se presenta un enfoque innovador, basado en la Teoría de la Torsión de Saint-Venant, para determinar la torsión de la fibra multinúcleo y compensar los errores debidos a la torsión durante la reconstrucción de la forma. La eficiencia del enfoque teórico fue verificada realizando una serie de pruebas de torsión en un sensor de forma, fabricado inscribiendo los sensores de FBGs en una fibra óptica multinúcleo torcida y siete núcleos. La investigación del comportamiento mecánico de los sensores ópticos de forma multinúcleo ha involucrado sinérgicamente diversas disciplinas: Mecánica del sólido, Fotónica, Estadística y Análisis de datos. Esta investigación multidisciplinaria ha surgido de la prolífica cooperación entre el Instituto de Ciencia y Tecnología del Hormigón (ICITECH) y el Instituto de Telecomunicaciones y Aplicaciones Multimedia (iTEAM) - Laboratorio de Investigación Fotónica (LIF) - de la Universidad Politécnica de Valencia (UPV), además de la valiosa colaboración con otros miembros del proyecto europeo ITN-FINESSE, al que pertenece este trabajo. Este trabajo de investigación puede permitir mejorar el comportamiento de los sensores de forma basados en fibra óptica multinúcleo y apoyar el desarrollo de nuevas geometrías de sensores, con un gran potencial para aplicaciones de control de la salud estructural. / [CA] Structural Health Monitoring (SHM) avalua quantitativament la integritat i el comportament de les infraestructures i permet desenrotllar plans eficaços de Maintenance and Rehabilitation (M&R), utilitzant les dades dels sensors. Optical Multicore Fiber (MCF) Shape Sensors oferixen una alternativa als mètodes tradicionals i permeten la reconstrucció de la forma de la deformació de les estructures de forma directa i en temps real, sense necessitat de models de càlcul o contacte visual i amb tots els avantatges de l'Optical Fiber Sensors (OFS) Technology. A pesar dels grans esforços en la investigació centrada en aquest tema per part dels grups d'investigació de tot el món, encara no s'ha realitzat una investigació exhaustiva que estudie els paràmetres que influïxen en el comportament d'aquestos sensors. En la primera part de la tesi es presenta un estudi numèric en què s'examinen els efectes de la precisió del mesurament de la tensió i els errors de posició del nucli en el comportament dels sensors de forma basats en fibra òptica multinucli per a definir la curvatura tridimensional, que és la base de la reconstrucció de la forma. L'anàlisi reproduïx el procés de mesurament de la tensió utilitzant el mètode de Monte Carlo (MC) i identifica una sèrie de paràmetres que exercixen un paper en el procés, entre ells la separació del nucli (distància entre els nuclis exteriors i l'eix del sensor), el nombre de nuclis i la mesura de la curvatura. Finalment, es va calibrar un conjunt de models de predicció ajustant els resultats de les simulacions per a predir el comportament dels sensors. A continuació, es proposa un estudi experimental per a avaluar el comportament dels sensors de forma basat en fibra òptica multinucli, amb especial atenció en la influència de la longitud dels sensors de deformació. Es van fabricar dos sensors de forma, inscrivint Fiber Bragg Gratings (FBG) amb longituds de 8,0 mm i 1,5 mm en els nuclis d'una fibra multinucli de set nuclis. Així, es va avaluar i es va comparar el comportament dels dos sensors en totes les fases necessàries per a la reconstrucció de la forma, incloent el mesurament de la tensió, el càlcul de la curvatura i la reconstrucció de la forma. Per a concloure, es presenta un enfocament innovador, basat en la Teoria de la Torsió de Saint-Venant, per a determinar la torsió de la fibra multinucli i compensar els errors deguts a la torsió durant la reconstrucció de la forma. L'eficiència de l'enfocament teòric va ser verificada realitzant una sèrie de proves de torsió en un sensor de forma, fabricat inscrivint els sensors de FBGs en una fibra òptica de set nuclis de filat múltiple. La investigació del comportament mecànic dels sensors òptics de forma multinucli ha involucrat sinèrgicament diverses disciplines: Mecànica del sòlid, Fotónica, Estadística i Anàlisi de dades. Aquesta investigació multidisciplinària ha sorgit de la prolífica cooperació entre l'Institut de Ciència i Tecnologia del Formigó (ICITECH) i l'Institut de Telecomunicacions i Aplicacions Multimèdia (iTEAM) - Laboratori de investigación fotònica (LIF) - de la Universitat Politècnica de València (UPV), a més de la valuosa col·laboració amb altres membres del projecte europeu ITN- FINESSE, al qual pertany aquest treball. Aquest treball d'investigació pot permetre millorar el comportament dels sensors de forma basats en fibra òptica multinucli i ajudar al desenrotllament de noves geometries de sensors, amb un gran potencial per a aplicacions de control de la salut estructural. / Floris, I. (2020). Optical Multicore Fiber Shape Sensors. A numerical and experimental performance assessment [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/148715