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11	Investigation on Cell-Cell Junctions by Inhibition of Na,K-ATPase Activity / Studie av cell till cell kontakter genom inhibering av Na,K-ATPas aktivitet Boström, Caroline January 2021 (has links) This thesis report investigates the effect on cellcell junction proteins when the Na,K-ATPase (NKA) is inhibited. The main goal is to develop an understanding of how the NKA activity regulates the cell junction proteins. The investigated proteins are the adherens junction protein ECadherin, and the tight junction proteins Occludin and Claudin7.The NKA is inhibited by introducing the cardiotonic steroid Ouabain to the cells. The treatment is tested for different time lapse and different concentrations. The results show that all proteins are down regulated when treated with high concentrations (500 nM) of Ouabain. ECadherin is up regulated when treated with lower concentrations (10 nM) of Ouabain while Claudin7 is down regulated at low levels. / Detta examensarbete undersöker effekten på cell-cell junctions när Na,K-ATPas (NKA) inhiberas. Målet med rapporten är att få en förståelse för hur NKA aktiviteten reglerar cell-cell junction proteinerna. Proteinerna som undersöks är adherens junction proteinet ECadherin, och tight junction proteinerna Claudin7 och Occludin. NKA inhiberas genom att cellerna behandlas med den kardiotoniska steroiden Ouabain. Behandlingen testas under olika tidsperioder och för olika koncentrationer. Resultaten visar att alla proteiner är nedreglerade när de behandlas med höga koncentrationer (500 nM) av Ouabain. ECadherin blir uppreglerad när det behandlas med lägre koncentrationer (10 nM) av Ouabain medan Claudin7 nedregleras vid låga nivåer. NaK-ATPase Ouabain Cell junctions ECadherin Occludin Claudin7 Western Blot Fluorescent Imaging Tight junctions Adherens Junctions Immunocytochemistry Ouabain NaK-ATPase ECadherin Occludin Claudin7 Western Blot Immunocytokemi Cellkontakter Fluorescernsmikroskopi Physical Sciences Fysik
12	Development of a Z-Stack Projection Imaging Protocol for a Nerve Allograft Selvam, Selvaanish 31 August 2018 (has links) No description available. Biomedical Engineering Optics Cellular Biology
13	The use of Gibson Assembly for DNA cloning / Användning av Gibson Assembly för att klona DNA Johansson, Samuel January 2022 (has links) This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. These plasmids were first amplified using PCR, and then cloned using Gibson-Assembly, and then transfected into live HEK293T or MDCK-II cells. After the transfection, the cells were examined in a microscope. The results showed no signal or localization for the cloned plasmids in their respective corresponding channel, 561 nm for the red fluorescent protein mCherry or 488 nm for the green fluorescent protein EGFP. The step that went wrong was the PCR step in the cloning process, since the backbone vector was not successfully amplified. The reasons for this was either that the backbone vector was too long, the primers regions were to rich with Guanine and Cytoseine, or the primers being too long. / Den här tesen kretsade kring kloningsprocessen för plasmider. Det gjordes försök att från plasmider klona in det röda fluorescerande proteinet mCherry, samt det gröna fluorescerande proteinet EGFP in i andra plasmider som innehöll olika cell-junction proteiner. Både det fluorescerande fragmenten och plasmid-vektorerna innehållande cell-junction proteinerna amplifierades med PCR. Sedan gjordes Gibson-Assembly som var själva kloningsmetoden. Efter det transfekterades HEK293T, samt MDCK-II celler med lösningen från Gibson-Assembly kloningen. Dessa celler undersöktes sedan i mikroskop. Resultatet visade inga tydliga signaler varken i 561 nm kanalen (mCherry), eller i 488 nm kanalen (EGFP), vilket betyder att kloningen inte fungerade. Steget som gick fel var PCR-steget i själva kloningsprocessen, då plasmid-vektorerna inte amplifierades. Anledningen till detta var antingen att själva plasmid-vektorerna var för långa, primer regionerna hade för mycket Guanin och Cytosin, eller att alla primers själva var för långa. Plasmid Primer Cloning PCR Gibson-Assembly Transfection Fluorescent protein Fluorescent imaging mCherry EGFP Zona-Occludens 1 Occludin alphaTubulin Cx-43-7 Plasmid Primer Kloning PCR Gibson-Assembly Transfektion Fluorescerande protein Fluorescerande avbildning mCherry EGFP Zona-Occludens 1 Occludin alphaTubulin Cx-43-7 Physical Sciences Fysik
14	Mesenchymal Stem Cell Immunomodulation Effects as Determined by Cryo-imaging Wuttisarnwattana, Patiwet 03 June 2015 (has links) No description available. Biomedical Research Experiments Medical Imaging Biomedical Engineering Immunology Computer Science stem cell mesenchymal stem cell bio-distribution co-localization cryo-imaging fluorescent imaging medical imaging graft-versus-host disease GVHD cell detection segmentation 3D visualization T-cell T-cell proliferation image processing

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