Made available in DSpace on 2015-03-26T13:53:08Z (GMT). No. of bitstreams: 1 texto completo.pdf: 393357 bytes, checksum: 48579cc6ca03f6886f75a7b153778817 (MD5) Previous issue date: 2009-02-18 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Brazil is one of the biggest producers of cellulose, paper and vegetal coal of the world, and the activities related to planted forests contribute to 3.5 % of the Gross Domestic Product (PIB) of the country. The eucalypt is the forest essence that stands out, with a planted area of 3.75 millions of hectares, and the state of Minas Gerais has about 28 % of this planted area. In Brazil, with the intensification of the clonal sivilculture, the management of genetic materials that are more productive and more nutritional demanding has increased the appearance of symptoms of micronutrients deficiency, mainly of B, and, more recently, of Cu and Zn, especially in areas of Cerrado. The aim of this study was to evaluate three methods of determining Cu and Zn availability (Mehlich-1, Mehlich-3 and DTPA pH 7.3) to eucalypts seedlings cultivated under greenhouse conditions, in samples of six soils from Minas Gerais state (one sample from João Pinheiro, two from Vazante, one from Três Marias and two from Curvelo) and the influence of the properties of the soil in the efficiency of these extractors. Were realized two experiments, one for each micronutrient. The treatments were arranged in a randomized block design, with four replications, in a factorial scheme of 6 x 5, corresponding to six soils and five doses of micronutrient. The doses were 0, 2, 4, 8 and 16 mg dm-3 of Cu and 0, 2.5, 5, 10 and 20 mg dm-3 of Zn, in the form of Cu and Zn chloride, respectively. After 70 days of growth, the plants were cut into two distinct portions, one being apical (apical third of all the branches of the plant) and the other being basal (the rest of the plant cut down near the ground level). The criticals levels of Cu and Zn in soils from Cerrado, to the growth of eucalypts seedlings, are equal or inferiors to 0.12 and 0.23 mg dm-3 by the Mehlich-1 and are equal or inferiors to 0.09 and 0.05 mg dm-3 by the DTPA, respectively. The concentrations of Cu and Zn in the soil and in the plant, as well as the respectives contents, increase with the elevation of the additional doses of these nutrients. The concentrations and the contents of Cu and Zn in the plant show close relation to the contents extracted by the three extractors. The concentrations of Cu and Zn extracted by Mehlich-1, Mehlich-3 and by DTPA were strongly correlated. The content of organic matter and the P- remanescent are the characteristics of the soils which most negatively influence in the recovering rates of Cu and Zn by the extractors Mehlich-1 and Mehlich-3, while for the DTPA, the recovering rates are more negatively influenced by the pH. The Mehlich-1 is the most recommended extractor to evaluate Cu and Zn availability to eucalypts seedlings. First because the method is easy to handle when compared to other methods, and second because it is a method which has already been used by the great majority of the labs in the country. / O Brasil é um dos maiores produtores de celulose, papel e carvão vegetal do mundo, e as atividades relacionadas às florestas plantadas contribuem com 3,5 % do Produto Interno Bruto (PIB) do país. O eucalipto é a essência florestal que se destaca, com uma área plantada de 3,75 milhões de hectares e o Estado de Minas Gerais possui em torno de 28 % dessa área plantada. No Brasil, com a intensificação da silvicultura clonal e o plantio de materiais genéticos mais produtivos e exigentes nutricionalmente têm aumentado o aparecimento de sintomas de deficiência de micronutrientes, principalmente de B, e, mais recentemente, de Cu e Zn, especialmente em áreas de Cerrado. Este trabalho teve por objetivo avaliar três métodos de determinação da disponibilidade de Cu e de Zn (Mehlich-1, Mehlich-3 e DTPA pH 7,3) para mudas de eucalipto cultivadas em casa de vegetação, em amostras de seis solos do Estado de Minas Gerais (um de João Pinheiro, dois de Vazante, um de Três Marias e dois de Curvelo) e a influência de propriedades do solo na eficiência desses extratores. Foram realizados dois experimentos, um para cada micronutriente. Os tratamentos foram arranjados no esquema fatorial 6 x 5, correspondendo a seis solos e cinco doses do micronutriente, com quatro repetições, em blocos ao acaso. As doses foram de 0, 2, 4, 8 e 16 mg dm-3 de Cu e de 0, 2,5, 5, 10 e 20 mg dm-3 de Zn, na forma de cloreto de Cu e Zn, respectivamente. Após 70 dias de crescimento, as plantas foram cortadas em duas porções distintas, sendo uma apical (terço apical de todos os ramos da planta) e a outra basal (restante da planta cortada rente ao solo). Os níveis críticos de Cu e de Zn em solos de Cerrado, para o crescimento de mudas de eucalipto, são iguais ou inferiores a 0,12 e 0,23 mg dm-3 pelo Mehlich-1 e iguais ou inferiores a 0,09 e 0,05 mg dm-3 pelo DTPA, respectivamente. Os teores de Cu e de Zn no solo e na planta, bem como os respectivos conteúdos, aumentam com a elevação das doses adicionadas desses nutrientes. Os teores e conteúdos de Cu e de Zn na planta mostram estreita relação com os teores extraídos pelos três extratores. Os teores de Cu e de Zn extraídos pelo Mehlich-1, Mehlich-3 e DTPA são altamente correlacionados entre si. O teor de matéria orgânica e o P-remanescente são características dos solos que mais influenciam negativamente nas taxas de recuperação de Cu e de Zn pelos extratores Mehlich-1 e Mehlich-3, enquanto que para o DTPA, as taxas de recuperação são mais influenciadas pelo pH. O Mehlich-1 é o extrator mais recomendado para avaliar a disponibilidade de Cu e de Zn para mudas de eucalipto, pela facilidade operacional deste método frente aos outros e por este já ser usado na grande maioria dos laboratórios do país.

Eucalyptus is the most planted forest genus in Brazil and worldwide. The eucalyptus plantations are established in various environments, and in the state of Rio Grande do Sul, new plantations are being established in the Pampa biome region. Therefore, there is need to use appropriate and correct genetic material nutritional management of stands. This study aimed to characterize nutritionally one Seed Production Area (APS) Eucalyptus benthamii, to six years in the city of São Francisco de Assis - RS, on the farm Estância das Oliveiras, owned by Stora Enso Florestal RS company. For the study, nine trees were harvested in three diameter classes, where samples of biomass were obtained above and below ground (leaves, branches, wood, bark, fruits and root) with subsequent determination of the dry mass and the concentration of macro and micronutrients. The total biomass in Seed Production Area was 47,59 Mg ha-1, with the following percentage of biomass: 62,14% in the wood, 10,19% in the branches, 8,26% in the bark, the leaves has 6,25%, 0,05% in the fruits and 13.11% in the roots. The stock of elements in the total biomass accumulation showed the following order of macronutrients: N > K > Ca > Mg > P > S and micronutrients to the order of distribution was as follows: Fe > Mn > Zn > B > Cu. The macronutrient concentrations in the biomass followed the sequence: wood > roots > leaves > branches > bark > fruit. For micronutrient concentrations follow the order: root > wood > branches > leaves > bark > fruit. The export of nutrients by the intensity of harvest of biomass components had the following distribution trend in the collection scenario 1 (30% removal leaves, twigs + 30% + 100% fruit): Mn> N> Cu> K> Mg> S> B> Ca> P> Zn> Fe Considering the scenario 2 (50% removal leaves, twigs + 50% + 100% fruit) harvesting the export of nutrients had the following distribution: Mn > N> Cu> K> Ca> Mg> S> B> P> Zn> Fe. For the scenario of harvest 3 (removal of 100% + 100% leaves and branches all fruit) export of nutrients presented following distribution: Mn> N> Cu> B> Mg> K> Ca> S> P> Zn> Fe / O Eucalyptus é o gênero florestal mais plantado no Brasil e no mundo. Os povoamentos de eucalipto estão estabelecidos nos mais variados ambientes, sendo que, no estado do Rio Grande do Sul, novas plantações estão sendo estabelecidas na região do bioma Pampa. Para tanto, há necessidade de utilização de material genético adaptado e correto manejo nutricional dos povoamentos. O presente estudo teve por objetivo caracterizar nutricionalmente uma Área de Produção de Sementes (APS) de Eucalyptus benthamii, aos seis anos, no município de São Francisco de Assis RS, na fazenda Estância das Oliveiras, pertencente à empresa StoraEnso Florestal RS. Para realizar o estudo, foram abatidas nove árvores, em três classes de diâmetro, onde as amostras da biomassa foram obtidas acima e abaixo do solo (folhas, galhos, madeira do tronco, casca do tronco, frutos e raiz), com posterior determinação da massa seca e a concentração dos macro e micronutrientes. A produção de biomassa total na Área de Produção de Sementes foi de 47,59 Mg ha-1, com a seguinte distribuição de biomassa: 62,14% na madeira do tronco, 10,19% nos galhos, 8,25% na casca do tronco, 6,26% nas folhas, 0,05% nos frutos e 13,11% nas raízes. O estoque dos elementos na biomassa total apresentou a seguinte ordem de acúmulo para macronutrientes: N > K > Ca > Mg > P > S e para os micronutrientes a ordem de distribuição foi a seguinte: Fe > Mn > Zn > B > Cu. A concentração dos macronutrientes na biomassa apresentou a seguinte ordem: madeira do tronco > raízes > folhas > galhos > casca do tronco > frutos. Para os micronutrientes as concentrações seguem a ordem: raízes > madeira do tronco > galhos > folhas > casca do tronco > frutos. A exportação de nutrientes pela intensidade da colheita dos componentes da biomassa, apresentou a seguinte tendência de distribuição no cenário de colheita 1 (remoção de 30% de folhas,+ 30% de galhos + 100% dos frutos): Mn > N > Cu > K > Mg > S > B > Ca > P > Zn > Fe. Considerando o cenário de colheita 2 (remoção de 50% de folhas, + 50% galhos + 100% dos frutos) a exportação de nutrientes teve a seguinte distribuição: Mn > N > Cu > K > Ca > Mg > S > B > P > Zn > Fe. Para o cenário de colheita 3 (remoção de 100% de folhas + 100% de galhos e todos os frutos) a exportação de nutrientes apresentou a seguinte distribuição: Mn > N > Cu > B > Mg > K > Ca > S > P > Zn > Fe.

En milieu forestier, la décomposition de la matière organique d’origine végétale et son assimilation par les microorganismes sont des processus essentiels au bon fonctionnement des sols et du cycle du carbone. Les mécanismes impliqués dans ces phénomènes de dégradation sont fortement contrôlés par les champignons saprotrophes qui sécrètent de nombreuses enzymes hydrolytiques afin d’accéder à leur principale source de nutriments qui se trouve sous la forme de polysaccharides (cellulose et hémicelluloses). L’hydrolyse enzymatique de ces polymères végétaux engendre une grande diversité de composés de faible poids moléculaire (mono- et oligosaccharides). Ces molécules pénètrent dans la cellule fongique via des systèmes de transporteurs membranaires dont la présence/absence et la spécificité de substrat contribuent à la versatilité métabolique de ces microorganismes du sol. La nature de l’essence forestière affecte fortement la diversité et la composition des communautés fongiques. Dans ce contexte, nous avons émis l’hypothèse que les communautés fongiques sélectionnées par les différentes essences forestières diffèrent entre elles par la diversité des mécanismes qu’elles mettent en place lors du processus de décomposition de la matière organique d'origine végétale et lors du transport des produits de dégradation ; et que de ce fait chaque communauté fongique est spécifiquement adaptée à la nature de la litière produite par l’espèce végétale considérée. Par des approches de séquençage haut-débit d’amplicons générés à partir d’ADNc environnementaux, nous avons analysé la diversité des transcrits codant des protéines fongiques impliquées à la fois dans la dégradation des polysaccharides végétaux et dans le transport des sucres issus de cette hydrolyse enzymatique au sein de sols localisés sous des forêts de hêtres et d’épicéas. L’analyse des données obtenues a mis en évidence des transcrits jusqu’alors inconnus et spécifiquement retrouvés pour plus 80% d’entre eux sous l’un des deux couverts végétaux conduisant ainsi à un effet significatif de l’essence forestière sur la composition des gènes exprimés par les communautés fongiques. Des transporteurs potentiellement spécifiques du mannose ont été détectés, pour la première fois, sous la forêt d’épicéas par une approche de crible fonctionnel dans la levure de banques d’ADNc environnementaux. Or, cette essence forestière est connue pour posséder d’importantes quantités de mannose au niveau de ses hémicelluloses. Les résultats obtenus au cours de cette thèse soulignent l’importance d’étudier la diversité fonctionnelle des communautés fongiques pour comprendre l’impact du couvert végétal sur leur composition et le fonctionnement des écosystèmes forestiers / The degradation of plant biomass is an essential process for the proper functioning of forest soils and terrestrial carbon cycling. Mechanisms involved in these processes are strongly controlled by saprotrophic fungi which secrete several hydrolytic enzymes to access at their primary nutrient sources found under the form of polysaccharides (cellulose and hemicelluloses). Enzymatic hydrolysis of plant polymers releases a high diversity of low molecular weight compounds (mono- and oligosaccharides). These molecules enter in fungal cell using transmembrane transporter systems. Consequently, the presence/absence and the substrate specificity of these transporters might contribute to the metabolic versatility of soil fungi. Several studies have demonstrated that tree species strongly affect diversity and composition of fungal communities. In this context, we hypothesized that the fungal communities selected by the different tree species expressed specific lignocellulolytic enzymes and sugar transporters; and thereby each fungal community was specifically adapted to the nature of litter produced by the tree species considered. We assessed, by the high-throughput sequencing of gene-fragments amplified from soil cDNA, the impact of tree species (Beech vs Spruce) on the diversity of genes encoding either lignocellulolytic enzymes or sugar porters expressed by soil fungi in two mono-specific forests. Our results revealed that most detected genes, encoding either lignocellulolytic enzymes or sugar transporters, have an unknown origin and are specifically found (for more than 80% of them) in one of the two forest soils. This work showed a significant “tree species effect” on the composition of functional genes expressed by soil fungi and suggests that beyond the species level, functional diversity of fungal communities must be addressed to better understand ecosystem functioning. Moreover, by using a functional metatranscriptomic approach, we identified functional transporter sequences differing with respect to their substrate specificities. From a spruce cDNA library, and for the first time, we identified high affinity or mannose specific transporters. Coincidently, as opposed to beech, spruce is indeed a tree species with a large proportion of mannose in its hemicelluloses

Forest ecosystems represent a huge reservoir of carbon. The volume of deadwood in managed forests is in the tens of m3 h-1, while unmanaged forests have the volume of deadwood in hundreds of m3 ha- 1. Deadwood is a substrate with a high potential for decomposition. However, deadwood is a specific habitat that has a high C/N ratio and changes due to ongoing decomposition. The C/N ratio decreases with increasing decay time. In addition to the C/N ratio, the pH also changes during the wood pulping process, due to the decomposition of plant biomass by fungi. Moreover, deadwood has a high impermeability. The main decomposers of dead wood are fungi. In addition to them, bacteria, which are numerous in deadwood, also contribute to decomposition. Bacterial communities that decompose woody biomass are affected by these factors, and their distribution is also affected by dead tree species. The aim of this study was to describe how bacterial communities associated with deadwood change during decay. The most significant factor in the decomposition by bacterial communities is the time of decay. Bacteria found in short-decaying wood (less than 16 years old) are more diverse than those found in long-decaying wood. Methylotrophic bacteria and N-fixing bacteria are common in short-decaying wood as well. N-fixing...

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Thesis (MScFor)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: A field trial of six replications was established to test the effect of various wood ash and fertilisers application rates on soil chemistry, tree nutrition and early growth rate of a clonal Eucalyptus grandis x urophylla stand. Wood ash from pulpmills is currently disposed of in landfills. Increasing costs and potential environmental risks have driven companies to investigate alternative disposal methods. Ash consists of a combination of carbonates, hydroxides and other calcium containing minerals that induce the liming effect if ash is applied to a soil. The trial was established near Richards Bay in October 2013 on a sandy soil with a low buffer capacity and a pH of approximately 5.5. The trial consists of four wood ash application rates in combination with three levels of fertiliser, viz. no fertiliser, 150 g conventional NPK fertiliser mixture, or 320g NPK controlled release mixture. Fertiliser mixtures and application levels were based on previous fertiliser trials in the region. Ash application rates for the field trial were based on a lab incubation study done with soil samples from Richards Bay, to which increasing amounts of lime were added. The study tested wood ash application rates of 0, 300, 600 and 1200 kg/ha. Field measurements were taken at 4 and 8 months after trial establishment. The primary objective was to investigate which application levels in combination with the type of fertiliser could be applied to soils without negatively affecting the stand nutrition or increase the levels of potentially harmful elements in the soil; thus investigating the feasibility of safely disposing wood ash on plantation soils as an alternative disposal method. Soil nutrient concentrations were not affected by individual wood ash treatments, but more a product of the time interval after the ash additions were made. Soil C, P, K+ and Mg2+ showed decreased concentrations from 4-8 months after establishment. Ca2+ concentrations increased in the same time interval. In addition, Na+ and B concentrations decreased from 4-8 months. Soil heavy metal concentrations for Cd, Hg, Cr and Pb, analysed for 0-1200 kg/ha ash rates, were well below toxic levels at both time intervals. Wood ash induced a temporary liming effect. Mean soil pH increased with 0.6 units for the period 0 - 4 months and decreased with 0.4 units at 4 - 8 months after trial establishment. Foliar nutrient analyses and assessment techniques revealed sub-optimal nutrient concentrations for P, K and Zn at 4 and 8 months of age. Concentrations were defined as sub-optimal, as none of the nutrients were below critical levels. Foliar heavy metal concentrations for Cd, Hg, Cr and Pb, measured at both time intervals, were less than 1mg/kg. The small concentrations found in this project were attributed to the low bioavailability of all four elements and were likely a product of the edaphic factors at Richards Bay, which was representative of a large greater portion of the Zululand coastal plain sites. The response in biomass index ranged between 13 % and 683 % relative to the control treatment (A0F0). Results showed that application of purely wood ash, or in combination with a supplementary N and P source increased growth up to 8 months after trial establishment for wood ash applications up to 1200 kg/ha. This project demonstrated that 1200 kg/ha wood ash can safely be disposed of on a typical Zululand coastal sand with little environmental risk and no supressed growth, provided that it is balanced with an appropriate NP fertiliser. / AFRIKAANSE OPSOMMING: ‘n Veldproef met ses herhalings is in Oktober 2013 uitgelê met die doel om die uitwerking van verskillende vlakke hout as en kunsmis toedienings op die grond-voedingstof status, boom-voedingstof status en die groei-tempo van ‘n Eucalyptus grandis x urophylla hibried plantasie te bestudeer. Hout as by pulpmeulens word tans weggegooi op stortingsterreine. Toenemende onkostes vir storting en die omgewingsrisiko’s gebonde aan stortingsterreine, dryf maatskappye om verbeterde en meer omgewingsvriendelike metodes te ondersoek om van die as ontslae te raak. Hout as bestaan uit ‘n reeks karbonate, hidroksiede en kalsium bevattende minerale en is verantwoordelik vir die bekalkingseffek op die grond na toediening. Die veldproef is geleë naby Richardsbaai op ‘n sanderige grond met n lae bufferkapasiteit en pH van ongeveer 5.5. Die proef het vier hout as vlakke getoets, gekombineer met drie vlakke van bemesting: geen, 150g konvensionele landbou kunsmis (CV) óf 320g beheerd-vrystellende kunmis (CRF). Die kunsmismengsels en vlakke van bemesting is gebaseer op bestaande of voltooide bemestingseksperimente in die streek. Hout as vlakke was bereken in gekontroleerde laboratorium toestande en gebaseer op ‘n inkubasie studie met grond monsters verkry vanaf Richardsbaai, waarby toenemende vlakke suiwer landboukalk gevoeg is. Die veldproef het hout as vlakke van 0, 300, 600 en 1200 kg/ha getoets. Veld metings is op 4 en 8 maande na behandeling geneem. Die primêre doelwit van die studie was om te bepaal watter vlak hout as en kunmis kombinasie toegedien kan word, sonder om die grond-voedingstof status negatief te beïnvloed of ‘n potensiële skadelike uitwerking op die plantasie groei te veroorsaak. Die uiteinde van die studie was om die haalbaarheid van hout as toedienings op plantasie gronde te bestudeer relatief tot die huidige praktyk van storting, insluitend die risiko van moontlike skadelike newe-effekte. Grondvoedingstatus was nie beduidend beïnvloed deur individuele hout as toevoegings nie, maar was eerder ‘n funksie van die tydsduur sedert behandeling. Grond koolstof, P anione, K+ en Mg2+ konsentrasies het beduidend afgeneem in die periode van 4 - 8 maande na behandling. Die Ca2+ konsentrasies het toegeneem tussen 4 en 8 maande en terselfdertyd het Na+ en B konsentrasies afgeneem. Die swaarmetaal status, spesifiek vir Cd, Hg, Cr en Pb, vir toevoegings van 0-1200 kg/ha hout as was beduidend laer as toelaatbare vlakke in gronde op albei tydsintervalle. Die hout as het ‘n tydelike toename in grond pH veroorsaak. Die gemiddelde pH het tussen 0 - 4 maande toegeneem met 0.6 eenhede en gedurende 4 - 8 maande afgeem met 0.4 eenhede. Blaarontledings en voedingstof assesseringsmetodes het sub-optimale konsentrasies vir P, K en Zn getoon op die ouderdom van 4 en 8 maande. Voedingstof konsentrasies is as sub-optimaal geklassifiseer, omdat konsentrasies nooit laer as kritieke waardes vir gebreksimptome was nie. Die inhoud van Cd, Hg, Cr en Pb in blare was aansienlik kleiner as 1 mg/kg op albei tydsintervalle. Die merkwaardige lae konsentrasies wat in die projek aangeteken is, word toegekryf aan die lae bio-beskikbaarheid van al vier elemente as gevolg van die edafiese faktore eie aan die Richardsbaai omgewing (en ook aan groot dele van die Zoeloelandse kusvlakte). Die groeireaksie (bepaal as biomassa indeks op ouderdom 8 maande) het gewissel van 13 % - 683 % groter as die kontrole behandeling (A0F0). Resultate het bewys dat toedienings van suiwer hout as, of hout as gekombineer met ‘n addisionele N en P kunsmisbron die groei postief beïnvloed tot op die ouderdom van 8 maande. Die studie het bewys dat 1200 kg/ha hout as veilig toegedien kan word op die sandgronde van die kusgebiede in Zululand, met minimale omgewingsrisiko en geen tekens onderdrukte groei nie, mits dit gebalanseer word met ‘n geskikte NP kunsmisbron.

Les sols forestiers contiennent des stocks de C importants et la gestion de tels espaces naturels est donc cruciale dans le contexte du changement climatique. Ce travail s’attache à comprendre quels leviers (facteurs environnementaux, pratiques sylvicoles) contrôlent le fonctionnement microbien des sols de peuplements représentatifs de Provence calcaire en considérant différentes échelles spatiales. La hiérarchisation des facteurs modulant le fonctionnement des sols forestiers a mis en évidence une structuration des communautés fonctionnelles microbiennes à l’échelle régionale par les conditions bioclimatiques (bioclimats sub-humide vs. humide), et une modulation des activités enzymatiques à l’échelle locale par la MO définie par le peuplement (Quercus ilex et Pinus halepensis vs. Q. pubescens et P. sylvestris en composition pure ou mixte). Les effets de la mixité forestière (décrite via les surfaces terrières et les densités des troncs) sur le fonctionnement du sol a été évaluée dans les deux contextes bioclimatiques: la complémentarité des essences en peuplements mixtes sous bioclimat humide favorise le recyclage de la MO et induit une diminution des stocks de C et N, cet effet non-additif des essences n’est pas observé sous bioclimat sub-humide. L’addition de différents types de rémanents (feuilles/aiguilles vs. bois) a été testée en mésocosmes : les équilibres entre les populations fongiques et bactériennes sont conditionnés par le ratio C/N, lui-même dépendant du type de rémanents apporté. Ainsi, ces résultats indiquent que les pratiques sylvicoles pourraient orienter les dynamiques des flux de C dans les sols forestiers. / Since forest soils can store large carbon amounts, the management of such ecosystem is of major importance considering the forecasted climate change. This work aims at understanding which drivers (environmental factors and sylvicultural practices) control soil microbial functioning in forest stands of Provence (South of France) considering different spatial scales. The hierarchization of drivers showed that microbial functional diversity is driven at the regional scale by bioclimatic conditions (sub-humid vs. humid bioclimate) and enzyme activities at the local scale by the organic matter print defined by stand composition (Quercus ilex and Pinus halepensis vs. Q. pubescens and P. sylvestris in pure or mixed stand). Mixed forest composition and structure (described through basal area and density of trunks of pines and oaks) influenced soil functioning differently depending on the bioclimate: the complementarity of species in mixed stands in humid bioclimate favoured organic matter recycling and induced a decrease in C and N stocks, while an additive effect of tree species was found in sub-humid bioclimate. Amendments with different types of logging residues (leaves / needles vs. wood) were tested in mesocosms: the balance between fungal and bacterial populations was conditioned by the C to N ratio, C and N amounts varying with the type of residue. Thus, these results indicate that forest management - through the choice of adapted practices - has the potential to drive microbial functioning in forest soils.

Forest harvest can have significant impacts on forest ecosystems that may influence the capacity of soils to sequester carbon (C). The microbial community controls decomposition, which is a critical process in partitioning litter- and root-C between CO₂ and storage in semi-permanent soil-C pools. The objectives of this study were to determine the effect of clear-cutting and stand age on: 1) temporal dynamics of soil microbial community (SMC) structure and physiological status; and 2) shifts among microbial functional groups in taking up ¹³C-labeled plant materials during decomposition. The experiment was conducted in Douglas-fir ecosystems within the Gifford Pinchot National Forest, Washington. We chose stands of three different ages: old-growth where trees are between 300 and 500 years old; an 8-year old stand; and a 25-year old stand. Phospholipid fatty acid (PLFA) profiling and ¹³C-PLFA labeling techniques along with the ratio of saturated to monounsaturated PLFAs and the ratios of cyclopropyl PLFAs to their monoenoic precursors as microbial physiological stress markers were utilized. Microbial PLFA profiles showed that SMC structure and physiological status was most affected by season and secondarily by time since clear-cutting. Total microbial biomass and bacterial and fungal biomass were significantly reduced in CC8 but not in CC25 sites relative to old-growth sites. Total microbial biomass concentration was lowest and the stress indicators were highest in August, which corresponded to low soil moisture and high temperatures. The relative amount of ¹³C incorporated into PLFAs was also influenced by stand age and ¹³C source (¹³C-labeled litter vs. ¹³C-labeled root material). A significantly greater amount of ¹³C was incorporated in CC8 samples compared to OG1 samples in five out of the seven sample dates. Additionally, a significantly greater proportion of ¹³C was incorporated into soil samples containing the ¹³C-labeled litter material relative to samples containing ¹³C-labeled root material in four out of the seven dates. In general, 18:lω9 and 18:2ω6,9 (common fungal biomarkers) had the greatest amount of ¹³C incorporation throughout the study period in both clear-cut and old-growth sites, indicating the important role of fungi in the decomposition of litter and root material and translocation of C within soil layers. / Graduation date: 2006

Ecosystems currently face widespread biodiversity losses and other environmental disturbances, such as climate warming, related to increased anthropogenic activities. Within this context, scientists consider the effects of such changes on the biodiversity, and hence on the activity, of soil microorganisms. Indeed, soil microorganisms mediate a wide range of soil processes. Currently, knowledge on soil microbial diversity is still limited, partially due to technical limitations. The advent of molecular-based analyses now allows studying the soil microbial diversity. These advances in the study of soil microbial communities have lead to a growing evidence of the critical role played by the microbial community in ecosystem functioning. This relationship is supposed to be relevant for narrow processes, regulated by a restricted group of microorganisms, such as the nitrification process. This PhD thesis aimed at studying ammonia oxidizing bacteria (AOB) community structure and richness as an integrated part of soil functioning. This research aimed at investigating the effect of aboveground plant diversity on ammonia oxidizing bacteria diversity and function in forest and grassland soils with focus on the influence of (a) functional group identity of grassland plants (legumes, grasses, forbs), (b) grassland plant species richness and (c) tree species, on AOB diversity and function. Another objective of this research was to study the effect of a 3°C increase in air temperature on AOB diversity and function. The link between AOB diversity and function (potential nitrification) is also investigated. For grassland ecosystems, a microcosm experiment was realized. An experimental platform containing 288 assembled grassland communities was established in Wilrijk (Belgium). Grassland species were grown in 12 sunlit, climate controlled chambers. Each chamber contained 24 communities of variable species richness (S) (9 S=1, 9 S=3 and 6 S=9).The grassland species belonged to three functional groups: three species of each grasses (Dactylis glomerata L., Festuca arundinacea SCHREB., Lolium perenne L.), forbs (non-N-fixing dicots; Bellis perennis L., Rumex acetosa L., Plantagolanceolata L.), and legumes (N-fixing dicots; Trifolium repens L., Medicago sativa L., Lotus corniculatus L.). Half of these chambers were exposed to ambient temperature and the other half were exposed to (ambient +3°C) temperature. One ambient and one (ambient+3°C) chambers were destructively harvested 4, 16 and 28 months after the start of the experiment. The influence of plant functional group identity on the nitrification process and on AOB community structure and richness (AOB diversity) was assessed in soils collected from the first two destructive amplings (chapter 2). The effect of plant species richness on AOB diversity and function was considered for soils sampled after 16 and 28 months (chapter 3). AOB function was determined by potential nitrification. AOB community structure and richness were assessed by polymerase chain reaction followed by denaturing gradient gel electrophoresis (DGGE) and sequencing of excised DGGE bands. I found that functional group identity can affect AOB community structure. In particular, the presence of legumes, both in monoculture or in mixture with forbs and grasses, lead to AOB community composition changes towards AOB clusters tolerating higher ammonium concentrations. This change in AOB community structure was only linked to increased potential nitrification under monocultures of legumes, when ammonium was supposed to be not limiting. This study revealed that physiological attributes of AOB and resource availability may be important factors in controlling the nitrification process. This research showed that the impact of plant species richness on the nitrification process could be mediated by the interactions between plants and AOB, through competition for substrate. A 3°C increase in air temperature did not affect AOB community structure, richness or function. In forest ecosystems, we studied the effect of tree species in forest sites located in Belgian and in the Grand-Duchy of Luxembourg covered each by several deciduous or coniferous tree species (Fagus sylvatica L., Quercus petraea (Mattuschka) Lieblein, Picea abies (L.) Karst, Pseudotsuga menziesii (Mirbel) Franco). We investigated the influence of these tree species on microbial processes (chapter 5) related to C and N cycling, particularly with emphasize on the nitrification process and on the diversity of AOB (chapter 6). The results showed that the effect of tree species on net N mineralization was likely to be mediated through their effect on soil microbial biomass, reflecting their influence on organic matter content and carbon availability. Influence of tree species on nitrification (potential and relative) might be related to the presence of ground vegetation through its influence on soil ammonium and labile C availability. AOB community structure was more site-specific than tree specific. However, within sites, AOB community structure under broadleaved trees differed from the one under coniferous trees. The effect on tree species on AOB was likely to be driven by the influence of tree species on net N mineralization, which regulates the substrate availability for AOB. The results also demonstrated that the relationship between AOB diversity and function might be related both to AOB abundance and AOB community structure and richness. This thesis showed no clear relationship between AOB community structure or richness and AOB function. However, we revealed that aboveground grassland plant richness, grassland plant functional groups and tree species influence AOB community structure and richness. Actuellement, les écosystèmes sont soumis à dimportantes pressions anthropiques et environnementales, pouvant aboutir à des pertes massives de biodiversité. Les scientifiques sinterrogent sur limpact de ces perturbations sur la diversité et, par conséquent, sur lactivité des microorganismes du sol. En effet, ceux-ci régulent de nombreux processus du sol. Actuellement, de nombreuses lacunes subsistent dans la connaissance de la diversité microbienne du sol. Celles-ci peuvent être partiellement attribuées aux difficultés méthodologiques associées à l'étude des micro-organismes du sol. Lavènement des techniques moléculaires nous permet de combler ces lacunes. Les avancées réalisées dans l'étude des communautés microbiennes du sol ont mis en évidence le rôle crucial joué par les communautés microbiennes dans le fonctionnement des écosystèmes. De plus, il semblerait que les processus régulés par un groupe restreint dorganismes, tel le processus de nitrification, soient plus sensibles à toute altération de la communauté. Lobjectif de cette thèse était détudier la structure de la communauté ainsi que la richesse (nombre de bandes DGGE) des bactéries oxydant lammoniac (AOB) comme une partie intégrante du fonctionnement des sols. Notre étude se focalisait sur linfluence de (a) différents groupes fonctionnels de plantes (graminées, légumineuses, dicotylédones), (b) communautés de plantes présentant une richesse spécifique croissante et (c) différentes essences forestières, sur la diversité (structure de la communauté et richesse des AOB) et la fonction des AOB. Cette recherche étudiait également limpact dune augmentation de température de 3°C sur ces paramètres. Létablissement dun lien éventuel entre la diversité et la fonction (nitrification potentielle) des AOB a aussi été envisagé. Concernant les écosystèmes prairiaux, nous avons réalisé une étude en microcosmes. Une plateforme expérimentale comprenant 288 communautés artificielles de plantes a été établie à Wilrijk (Belgique). Cette plateforme consistait en 12 chambres, dont une moitié était à température ambiante et la seconde était à température ambiante augmentée de 3°C. Chaque chambre contient 24 communautés de plantes de richesse spécifique variable (9 S=1, 9 S=3 et 6 S=9). Les communautés de plantes sont créées avec 9 espèces de plantes appartenant à trois groupes fonctionnels : 3 espèces de graminées (Dactylis glomerata L., Festuca arundinacea SCHREB., Lolium perenne L.), de légumineuses (dicotylédones fixatrices dazote ;Trifolim repens L., Medicago sativa L., Lotus corniculatus L.), et de dicotylédones non fixatrices dazote (Bellis perennis L., Rumex acetosa L., Plantago lanceolata L.). Les sols issus dune chambre à température ambiante et dune chambre à température ambiante augmentée de 3°C ont été échantillonnés, respectivement, 4, 16 et 28 mois après le début de lexpérimentation. Linfluence des groupes fonctionnels de plantes sur le processus de nitrification ainsi que sur la structure de la communauté et la richesse des AOB a été mesuré sur les sols issus des deux premiers échantillonnages (chapitre 2). Nous avons mesuré leffet de la richesse croissante en plantes sur la diversité et lactivité des AOB sur les sols échantillonnés après 16 et 28 mois dexpérimentation (chapitre 3). La structure de la communauté ainsi que la richesse des AOB ont été évaluées à laide dune amplification spécifique par réaction de polymérisation en chaîne (PCR) de lADN génomique extrait du sol suivie par une séparation par électrophorèse sur gel dacrylamide en présence dun gradient dénaturant (DGGE). Nous avons identifié les différentes AOB présentes par séquençage des bandes DGGE excisées. Nos résultats ont montré que les différents groupes fonctionnels peuvent affecter la structure de la communauté des AOB. En particulier, la présence de légumineuses, aussi bien en monoculture quen mélange avec des graminées ou des dicotylédones non fixatrices dazote, provoque des changements au sein de la structure de la communauté des AOB, favorisant la présence de clusters tolérants des concentrations en ammonium plus élevées. Ces changements de la structure de la communauté des AOB sont liés à des augmentations de la production potentielle de nitrates (nitrification potentielle) quand lammonium est supposé être non limitant. Cette étude révèle que la physiologie des AOB ainsi que la disponibilité en substrat peuvent être des facteurs majeurs intervenant dans le contrôle du processus de nitrification. Cette recherche montre que linfluence de la richesse spécifique des plantes sur la nitrification pourrait dépendre des interactions entre les plantes et les AOB via la compétition pour le substrat. Une augmentation de la température de lair de 3°C na pas influencé les richesse, structure de la communauté ou les fonctions des AOB. Pour les écosystèmes forestiers, nous aborderons leffet de différentes essences forestières (Picea abies (L.) KARST, Fagus sylvatica L., Quercus petraea LIEBLEIN ; Pseudotsuga menziezii (MIRB.) FRANCO) dans différents peuplements au Grand Duché du Luxembourg et en Belgique. Nous avons étudié l'influence de ces essences forestières sur les processus microbiens (chapitre 5) liés aux cycles du carbone et de lazote, en particulier leur effet sur le processus de nitrification et la diversité des AOB (chapitre 6). Notre étude révèle que linfluence des essences forestières sur la minéralisation nette de lazote pourrait être attribuable à leur effet sur la biomasse microbienne, reflétant ainsi leur effet sur la teneur en matière organique et la disponibilité en carbone. Limpact des essences forestières sur la nitrification (à la fois sur la nitrification relative et sur la nitrification potentielle) serait conditionné par la présence de végétation au sol, en raison de linfluence de celle-ci sur la disponibilité en ammonium et en carbone labile. Nous avons observé que la structure de la communauté des AOB était plus spécifique aux sites quaux essences forestières. Cependant, au sein dun site, elle différait sous feuillus et sous conifères. Les essences forestières influenceraient la structure de la communauté des AOB au travers de limpact quelles ont sur la minéralisation nette de lazote qui régule, quant à elle, la disponibilité en ammonium. Cette recherche démontre que le lien observé entre la diversité et la fonction dépendrait la fois de labondance, de la structure de la communauté et de la richesse des AOB. Cette thèse na révélé aucune relation claire entre la structure de la communauté ou la richesse des AOB et leur fonction. Par contre, nous avons observé que la richesse spécifique et les groupes fonctionnels de plantes prairiales et les essences forestières affectent la structure de la communauté et la richesse des AOB.

Thesis (MScFor)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: In South Africa fertilizer applications at establishment is a common practice in the forestry industry. Recommendations are based on past research with conventional sources (CV) and as a result there have only been slight improvements in additional plantation production over recent years. The objective of this study was to investigate initial stand responses in terms of leaf are index (LAI), foliar nutrient content, biomass index (BI) and volume growth to the application of controlled release fertilizers (CRF) at re-establishment. Nitrogen (N), phosphorus (P) and potassium (K) were applied in a three way factorial combination to Pinus radiata at planting on a site in the Western Cape. The design was replicated five times across the study area. N (CRF) and P (CV) were applied in a two way factorial combination at planting to two Eucalyptus grandis x urophylla hybrids and Eucalyptus dunnii across three sites, one ex-agricultural and two re-establishment sites, on the Zululand coastal plain and the Midlands region. The design was replicated nine times on each study site. Harvesting residues were burnt on the eucalypt sites prior to establishment and may have had an impact on the responses found. An outbreak of Fusarium circinatum on the P. radiata site resulted in unexpected and extremely variable responses. An attempt to quantify the effect of the disease had limited success. The mean BI of the control treatment reached 25. The best CV and CRF treatments yielded improvements of 42 % and 83 % in BI over the control respectively, with only the CRF treatment difference being significant. Foliar analysis results revealed marginal to deficient concentrations of P and Mg being alleviated by the treatments in question. On the KZN Zululand sites there was a marked response of the hybrids to N with P having an additive effect on volume growth, LAI and foliar N content. Application of 120 g N and 20 g P per tree on the ex-agriculture site produced a volume of 8 m3 ha-1 at one year of age, a significant increase of 118 % and 80 % over the control and best CV treatment respectively. Application of 80 g N and 20 g P per tree, on the re-establishment site, yielded a volume of 24.6 m3 ha-1 at one year which equates to a significant 39 % and insignificant 7 % additional volume at one year over the control and best CV treatment respectively. A non-significant suppressive effect was found with 20 g CV P application only. At the KZN Midlands site, the major early response in height were to P application. Applications of 20 g CV P per tree, resulted in a mean height of 162.6 cm at seven months of age, a significant 28 % higher than the control. There was no significant effect of 80 g CRF N and 20 g CV P per tree respectively. CRF N applications up to 120 g per tree provide additional growth over recommended CV applications on coastal Zululand sites with low organic carbon (OC) content. On the KZN Midlands site with higher OC and clay content, early responses were limited to P fertilization only regardless of the fertilizer source. / AFRIKAANSE OPSOMMING: Dit is algemene praktyk in die Suid Afrikaanse bosbou industrie om kunsmis tydens aanplanting toe te dien. Hierdie aanbeveling is gebasseer op navorsing resultate met konvensionele bronne (CV), gevolglik was slegs ‘n klein toename in bykomende plantasie produksie gemeet die afgelope paar jaar. Die doelwit tydens hierdie studie is om die aanvanklike reaksie van die bome in terme van blaar oppervlak indeks (LAI), blaar-voedingstof inhoud, biomassa indeks (BI) en volume op die toediening van beheerde vrylating kunsmis (CRF) tydens aanplanting vas te stel. Stikstof (N), fosfor (P) en kalium (K) is in drie-ledige kombinasie aan Pinus radiata op ’n plantasie in die Weskaap toegedien. Die ontwerp is vyf keer in die studiegebied herhaal. N en P is in twee-ledige kombinasie aan twee Eucalyptus grandis x urophylla hibriede en Eucalyptus dunnii op drie groeiplekke, een eks-landbou en twee eks-bosbou, op die Zoeloeland kusvlakte en in die Natalse Middellande toegedien. Die ontwerp is nege keer in elke studiegebied herhaal. Oesreste is voor aanplanting op die Eucalyptus groeiplekke verbrand. Die voorkoms van Fusarium circinatum op die P. radiata groeiplek het onverwagte en hoogs uiteenlopende reaksies tot gevolg gehad. ’n Poging om die effek van die siekte te kwantifiseer, was slegs gedeeltelik suksesvol. Die gemiddelde BI van die kontrole behandeling, was 25. Die beste CV en CRF behandeling het onderskeidelik 42 % en 83 % hoër BI as die kontrole groep gehad, waarvan slegs die CRF behandeling beduidend was. Blaarontleding het gewys dat daar marginale of ontoereikende konsentrasies van P en Mg was. In Zoeloeland het die Eucalyptus hibriede ’n beduidende reaksie op N en P getoon met meer volume groei, LAI en N inhoud. Die toediening van 120 g N en 20 g P per boom op die eks-landbou groeiplek het ’n volume van 8 m3 ha-1 op eenjarige ouderdom tot gevolg gehad. Dis beduidend beter met 118 % en 80 % onderskeidelik vir die kontrole en beste CV behandeling. Die toediening van 80 g N en 20 g P per boom op die hervestigde eks-bosbou groeiplek, het ’n volume van 24.6 m3 ha-1 op eenjarige ouderdom tot gevolg gehad. Dit is 39 % beduidend en 7 % onbeduidend addisionele volume op eenjarige ouderdom vir onderskeidelik die kontrole groep en beste CV behandeling. ’n Onbeduidende depressie effek is met die alleen toediening van 20 g CV P gevind. In die Natalse Middellande groeiplek het die toediening van P ‘n vroeë reaksie in hoogte groei veroorsaak. Die toediening van 20 g CV P per boom, het ’n gemiddelde hoogte van 162.6 cm op die ouderdom van sewe maande tot gevolg gehad. Dit is 28 % beduidend hoër as die kontrole. Die toediening van 80 g CRF N en 20 g CV P per boom was onbeduidend. Toedienings van CRF N tot en met 120 g per boom het in die kusgebiede van Zoeloeland met ‘n lae organiese koolstof (OC) inhoud, groter groei as die aanbevole CV toedienings gehad. Die Middellande groeiplek met ’n hoër OC en klei inhoud, was die vroeë reaksie alleenlik beperk tot P bemesting.