INTRODUÇÃO: A urbanização em bacias hidrográficas contribui para a eutrofização antrópica dos seus corpos d´água, propiciando proliferações de algas tóxicas como as cianobactérias. Existe um padrão característico de sazonalidade nas florações de algas e comunidades de cianobactérias, embora estas algas possam estar presentes ou mesmo dominantes, na maior parte do ano, trazendo problemas práticos associados à elevada biomassa de cianobactérias e potenciais ameaças à saúde pelo aumento de toxinas. A hipótese desta tese é de que as variações do tempo e do clima podem influenciar a qualidade da água de abastecimento ao propiciar florações das algas tóxicas, cianobactérias, que geram toxinas dificilmente filtradas pelos mecanismos comuns, gerando um problema de saúde pública que pode ser agravado com as mudanças climáticas conjuntamente o fenômeno de urbanização crescente nos países de economia emergente. OBJETIVO: O objetivo deste estudo foi investigar qual a influência do tempo e do clima na qualidade da água de abastecimento público de bacias urbanizadas através do indicador de algas tóxicas chamadas cianobactérias. MATERIAL E MÉTODOS: Para tanto investigou-se em primeiro lugar qual o contexto das proliferações de cianobactérias em reservatório de abastecimento público e suas consequências bem como qual o estado da arte sobre a qualidade da água de reservatórios eutrofizados em bacias urbanizadas, foi elaborado um mapeamento bibliográfico para saber qual a extensão do problema e do que se sabe a respeito. E, por fim, correlacionou-se a influência do tempo e do clima na qualidade da água para abastecimento no caso da bacia urbanizada da Represa Guarapiranga, localizada ao sul da Cidade de São Paulo, com vistas à previsibilidade e prevenção do fenômeno das florações das cianobactérias. RESULTADOS E DISCUSSÃO: Chegando-se a conclusão de que a pressão antrópica e o uso do solo são um fator determinante para a eutrofização de reservatórios de abastecimento e que, por tanto, bacias hidrológicas ocupadas pelo espraiamento urbano são particularmente propensas a apresentar reservatórios de água contaminados com algas toxicas, ao mesmo tempo que são as mais visadas para abastecer a população em seu entorno. O mapeamento bibliográfico mostrou que a proliferação de cianotoxinas em reservatórios eutrofizados é prevalente em diferentes latitudes e climas. A modelagem de correlação teve sucesso em explicar de 16% à 30% das florações na Guarapiranga de acordo com episódios de Irradiação e de precipitação pluviométrica, sendo resultados coerentes com a literatura, embora sejam baixo para gerar um modelo preditivo. CONSIDERAÇÕES FINAIS: A pressão antrópica da urbanização de bacias hidrográficas sem planejamento ou infra-estrura sanitária se mostrou uma questão de Saúde Pública e Saúde Urbana. As florações de cianobactérias se apresentaram como fenômenos presentes em diferentes latitudes, climas e graus de organização do espaço, mostrando assim serem uma questão global de saúde pública. A modelagem para o caso de São Paulo resultou em valores de explicação insuficientes para gerar um modelo preditivo do fenômeno das florações, evidenciando o sistema complexo de interação ambiente-ocupação humana-uso do solo- clima, exigindo assim que sejam acrescentadas demais variáveis ao modelo. / INTRODUCTION: Urbanization in hydrographic basins contributes to the anthropic eutrophication of their water bodies, propitiating toxic algae proliferation such as cyanobacterias. There is a characteristic pattern of seasonality in algae blooms and cyanobacteria communities, although theses algae may be present or even dominant during most part of the year. They cause practical problems associated with high biomass volumes, and potential health threats by the rise of toxins. This thesis\' hypothesis is that weather and climate variations can influence the quality of drinking water supply, because toxins are hardly filtered by ordinary mechanisms. The problem can be aggravated by climate change conjointly with the rapid widespread of urbanization in low and middle income countries. OBJECTIVE: This study\'s aim was to investigate the influence of weather and climate variables on the quality of water from the public drinking water supply system of an urbanized watershed through the indicator of toxic algae - denominated cyanobacterias. MATERIALS AND METHODS: First, the cyanobacterias\' blooms in eutrophic reservoir and its\' consequences, was studied by literature review and by an exploratory analysis of monthy weather varables and cyanobacterias data. Secondly, a bibliographical mapping review was drafted in order to know the extension of the problem and what is known regarding it. Finally, data of weather and climate were correlated with the quality of supple water of the, Guarapiranga reservoir, located on the south of São Paulo metropolitan area, acknowledging predictability and prevention of the phenomenon of cyanobacteria bloom. FINDINGS AND DISCUSSION: The conclusion was that the anthropic pressure and land use are determinant factors to the eutrophication of supply reservoir and, therefore, hydrological basins occupied by urban sprawling are particularly at risk of presenting water reservoirs contaminated with toxic algae. They are the most targeted to supply water to their neighbor population. The bibliographic mapping showed that the proliferation of cyanotoxins in eutrophized reservoirs is prevalent in different latitudes and climates. The correlation modeling was successful in explaining 16% to 30% of blooms in Guarapiranga according to episodes of irradiation and low precipitation, matching results found in literature, although they are low for the creation of a predicative model. FINAL CONSIDERATIONS: The anthropic pressure of urbanization on hydrographic basins without planning or sanitary infrastructure guidelines was shown to be a matter of public and urban health. Cyanobacterias\' blooms was shown to be a phenomena present in different latitudes, climates and degrees of space organization, proving to be a matter of global public health. The modeling for the case of São Paulo has resulted in insufficient explanation values to generate a predictive model of the phenomena of flowering, indicating the complex interaction system involving environment, human occupation, land use and climate, and demanding that more variables be acknowledged in the model.

Secretion of mucus by epidermal goblet cells is an important first line of defense for teleosts. It protects them against many of the biological, physical and chemical insults they encounter in their environment. This project monitored changes in hemoglobin concentration in epidermal mucus and in the density, diameter and mucus quality of epidermal goblet cells in the mummichog, Fundulus heteroclitus, following exposure to creosote-contaminated sediment. Male fish (200) were exposed for 13 days in flowthough aquaria to either an uncontaminated reference sediment or contaminated sediment containing 30% Elizabeth River sediment. Fish were sampled on Days 0, 3, 7 and 13. The hemoglobin content of their mucus was assessed using a commercial hemoglobin test strip. Their condition index was determined and a portion of their ventral skin was mounted after sequential staining by alcian blue and periodic acid Schiff s reagent. The proportion of the mucin types present in the goblet cells, differentiated by the stains, was determined using light microscopy (600x). The aromatic compound concentrations in test aquaria effluents decreased significantly during the experiment, probably as a result of a reduction in resuspension of the sediment by the fish. The condition index was lower and the mortality rate and occurrence of epidermal lesions were higher in the treated fish than in the control fish. The hemoglobin content in the epidermal mucus of treated fish was significantly higher than in control fish. Significant reductions in both size and density of goblet cells observed in treated fish suggested a mucus secretion rate exceeding its production rate. Significant variations in mucin types occurred in both treatments, most likely as a result of the transfer of the fish from the holding tank to the experimental aquaria. Significant changes in mucin types between treatments did not occur until Day 13 and are not believed to be directly related to the creosote present in the treatment sediment.

Shoreline hardening and construction of restoration oyster reefs are occurring at rapid rates throughout Chesapeake Bay and little research has been conducted to determine whether installment of a hardened shoreline and oyster reef placement has an effect on the surrounding benthic infaunal communities. I investigated the effects of shoreline development and oyster reefs on benthic communities in Lynnhaven, Virginia. Throughout Lynnhaven, I determined the effects of shoreline type (natural marsh, oyster reef, rip-rap and bulkhead), sediment grain size, Total Organic Carbon/Total Nitrogen (TOC/TN) of the sediment, and predation (caging study) on density, biomass, and diversity of benthic infauna. An information-theoretic approach using Akaike’s Information Criterion (AIC) was used. Of the variables measured, shoreline type was the best predictor of benthic infaunal density (highest density at oyster reefs and lowest at bulkheads), while sediment composition (grain size and TOC/TN) and predators were the best predictors of biomass and diversity. Lynnhaven is a polyhaline, shallow, semienclosed, natural marsh-dominated system with high overall productivity, which could be masking any small-scale disturbances due to shoreline hardening at the sites. A Before-After-Control-Impact (BACI) study was completed at two sites (Eastern Branch and Linkhorn Bay) in Lynnhaven to examine the benthos before and after placement of oyster reefs. Replicate samples for benthic infauna, sediment grain size, and sediment TOC/TN were taken before and one year after reef placement. Based on the AIC analysis, oyster reefs had a positive effect on infaunal density at the Linkhorn Bay site after one year, mainly attributed to an influx of the bivalve Gemma gemma. The density increase occurred even with a decrease in polychaete density. There was no change in infaunal biomass or diversity at this site. At the Eastern Branch site, there was no effect of oyster reefs on density, biomass, or diversity. To characterize the benthos prior to reef placement, benthic samples were collected at two sites (Eastern Branch and Linkhorn Bay) in Lynnhaven. Four reef types (oyster shell, rip-rap, concrete modules, and reef ball) were deployed at both sites. Oyster production values for existing oyster reefs were used to estimate expected oyster production on each reef type. Biomass estimates and published P:B ratios for each taxa were used to calculate secondary production for benthic infauna and oysters. Lost benthic production due to each reef type at both sites was compared to estimated oyster production on each reef type to determine if each reef compensated for benthic production lost by placing the reefs on top of the benthos. Oyster production on oyster shell and reef ball reefs compensated for benthic production lost due to placement of the reefs at both sites. Oyster productivity on rip-rap and concrete module reefs did not compensate for lost benthic production at the highly productive Eastern Branch site, and barely compensated for lost benthos at the lower productivity Linkhorn Bay site. The preservation of natural marsh and use of the proper types of oyster reefs could help maintain the high productivity of both the benthic community and the Lynnhaven system itself.

The research projects presented in this dissertation used multiple tagging technologies to examine the movements, growth, and mortality rates of summer flounder tagged and released in Chesapeake Bay. In the first two chapters, I used acoustic, archival, and conventional tags to examine the behavior of summer flounder on different spatial scales. Investigating the movement behavior of individuals on different scales is an important step towards understanding how large-scale distributions of a population are established. Based on the observed behaviors of summer flounder, I hypothesize that the movements of these fish are primarily related to foraging behavior while they are resident in Chesapeake Bay. In the third chapter, I use growth models to investigate hypotheses regarding recreational angler noncompliance with minimum size regulations in Virginia. Angler noncompliance with management regulations can severely degrade the ability of fishery managers to prevent overexploitation of fish populations. Using a growth model fit to recreational angler mark-recapture data, I demonstrate that recreational anglers in Virginia responded to changes in summer flounder management regulations, but considerable levels of noncompliance were detected in years when management agencies drastically increased the minimum size regulations. In the final chapter, I attempt to estimate natural and fishing mortality rates of summer flounder using conventional mark-recapture data collected by an angler tagging program. These mortality rates were estimated using a Barker model, which is a generalization of the Cormack-Jolly-Seber tagging model. Results from this study indicated that sublegal summer flounder experience different emigration or mortality processes than do larger fish. Furthermore, handling and tagging mortality rates of summer flounder were much larger than the recreational discard mortality rate currently used in the stock assessment, implying that the recreational discard mortality rate should be reexamined. The research presented in this dissertation provides information that could be used by management agencies to further understand the behavior of summer flounder, and how to most effectively manage this population.

The index-removal method estimates abundance, exploitation and catchability coefficient, given surveys conducted before and after a known removal. The method assumes a closed population between surveys. Index-removal has seldom been applied due to its strong assumption of constant survey catchabilities. This work generalizes the method to allow multiple years of data to be incorporated, and the assumptions of the original model to be relaxed. If catchability is constant across years, precision can be improved by analyzing multi-year data simultaneously. Two multiple-year models were developed: the first, 1qIR, assumes constant catchability within and among years; the second, 2qIR, allows catchability to change between surveys within years, but assumes survey-specific catchability constant across years. The new models were tested by Monte Carlo simulation then applied to data from two southern rock lobster (Jasus edwardsii) populations. The 1qIR model produced reasonable estimates in one application, but the 2qIR model was required to produce reasonable estimates for the second population. A likelihood ratio test found 1qIR to be the most parsimonious model, even when, the assumption of constant survey catchability appeared to be violated. In that case, diagnostic plots suggested that the 2qIR model provided the most reliable estimates. However, when the constant catchability assumption is tenable, the 1qIR model offers the greatest precision for parameter estimates. Size- and sex-specific heterogeneity of catchability introduces bias in model estimates. Field experiments were performed to test whether the catchability of small lobster was constant for southern rock lobster during two seasons when fishing occurs. No evidence of heterogeneous catchability was observed during the spring. However, significantly more small lobster were caught in control traps and traps seeded with one large adult male lobster than were caught in traps seeded with one large adult female during the summer, when females are preparing to molt and reproduce in Tasmania. Because heterogeneous catchability occurred during the summer, but not the spring, an index of recruitment based on the catch of lobsters one molt size below legal size might be developed for the spring, however, more sampling is needed to resolve the annual timing of sex- and size-specific catchability changes.

No description available.

An investigation into the carbon dynamics and weathering processes occurring in Icelandic glacial-fed streams was conducted during the spring to summer seasonal transition in June of 2017. Four major outlet rives were sampled from the glaciers of Gígjökull, Steinsholtsjökull, Sólheimajökull, and Falljökull. Markarfljót, the major river that Gígjökull, Steinsholtsjökull, and many other glaciers drain into, was also sampled. Longitudinal sampling occurred at all sites to capture downstream trends in the hydrogeochemistry and carbon dynamics. Distinct differences in geochemistry between glacier surface meltwater, sub-glacial waters, pro-glacial lake water, and post-mixed downstream samples were evident in the data. Glacier surface streams were characterized by relatively colder water temperatures, lower specific conductivity, lower total dissolved solids (TDS) and ion concentrations, and more enriched δ13CDIC values than downstream samples. The THINCARB model was used to calculate the total dissolved inorganic carbon (TDIC), excess partial pressure of carbon dioxide (EpCO2), and percent contribution to TDIC by bicarbonate (HCO3), carbonate (CO3), and carbonic acid and dissolved CO2 (H2CO3). All sites showed a slight decreasing trend in DIC and EpCO2 downstream. The calculated CO2 flux ranged from 1.14 × 107 g/yr to 2.80 × 109 g/yr. The DIC flux ranged from 6.81 × 107 g/yr to 8.44 × 109 g/yr. The average carbon within the CO2 fluxing in these rivers accounts for 0.0004% of the annual, global flux of carbon. The δ13C values were the most variable throughout the study and indicate there are multiple sources influencing the river downstream. This study suggests that, despite previous assumptions and estimations, these glacial-fed rivers act as sources of CO2; however, the samples from this study only provide a snapshot into the carbon flux dynamics during the Spring to Summer seasonal transition. In most samples, HCO3 was the dominant species contributing to DIC content within the rivers, suggesting that DIC is being transported to the ocean as HCO3 but sourced to the atmosphere as CO2. By acting as sources of CO2 to the atmosphere, the process of glaciers melting, which drive geochemical processes within the rivers, are contributing to a positive feedback loop with respect to global warming.

The Sierra Nevada snowpack is vital to the water supply of California, the world’s sixth largest economy. Though tree ring and instrumental records show the dramatic influence of environmental change on California’s hydroclimate over the last millennium, few proxy archives assess winter precipitation variability farther back in time. Here, we use diatoms from a ~3,200 yr. old sediment core to reconstruct the paleolimnology of June Lake, a hydrologically closed glacial lake in the eastern Sierra Nevada. We test the hypothesis that limnologic and climatic changes control diatom assemblages at June Lake. Fossil diatom assemblages from June Lake sediments chiefly consist of the planktic genera Stephanodiscus and Lindavia; their relative abundances in sediments are controlled by lake response to changes in the length of the winter season. We establish a Lindavia:Stephanodiscus index to infer winter length; our results indicate three periods where winter seasons are longer than average: ~3.2-2.9 ka, ~2.2-1.7 ka, and ~0.6 ka-0.05 ka. Over the last ~100 yrs., June Lake has experienced stronger water column stratification and an expansion of the available benthic diatom habitat, indicating significantly warmer winters and lower lake levels. It is possible that this change is the result of anthropogenic climate warming.

In this thesis, the implementation and results of studies into the effect of pH on the kinetics of various iron transformations in natural waters are described. Specific studies include i) the oxidation of Fe(II) in the absence and presence of both model and natural organic ligands, ii) the complexation of Fe(III) by model organic compounds, and iii) the precipitation of Fe(III) through the use of both laboratory investigations of iron species and kinetic modeling. In the absence of organic ligands, oxidation of nanomolar concentrations of Fe(II) over the pH range 6.0 -- 8.0 is predominantly controlled by the reaction of Fe(II) with oxygen and with superoxide while the disproportionation of superoxide appears to be negligible. Oxidation of Fe(II) by hydrogen peroxide, back reduction of Fe(III) by superoxide and precipitation of Fe(III) have been shown to exert some influences at various stages of the oxidation at different pH and initial Fe(II) concentrations. In the presence of organic ligands, different effects on the Fe(II) oxidation kinetics is shown with different organic ligands, their initial concentrations and with varying pH. A detailed kinetic model is developed and shown to adequately describe the kinetics of Fe(II) oxidation in the absence and presence of various ligands over a range of concentrations and pH. The applicability of the previous oxidation models to describe the experimental data is assessed. Rate constants for formation of Fe(III) by a range of model organic compounds over the pH range 6.0 -- 9.5 are determined. Variation of rate constants for Fe(III) complexation by desferrioxamine B and ethylenediaminetetraacetate with varying pH is explained by an outer-sphere complexation model. The significant variation in rate constants of Fe(III) complexation by salicylate, 5-sulfosalicylate, citrate and 3,4-dihydroxylbenzoate with varying pH is possibly due to the presence of different complexes at different pH. The results of this study demonstrate that organic ligands from different sources may influence the speciation of iron in vastly different ways. The kinetics of Fe(III) precipitation are investigated in bicarbonate solutions over the pH range 6.0 -- 9.5. The rate of precipitation varies by nearly two orders of magnitude with a maximum rate constant at a pH of around 8.0. The results of the study support the existence of the dissolved neutral species Fe(OH)30 and suggests that it is the dominant precursor in Fe(III) polymerization and subsequent precipitation at circumneutral pH. Variation in the precipitation rate constant over the pH range considered is consistent with a mechanism in which the kinetics of iron precipitation are controlled by rates of water exchange in dissolved iron hydrolysis species.

The aim of this thesis is to use phylogenetic analyses of mitochondrial DNA to investigate the biogeography and evolutionary relationships within the freshwater shrimp family Atyidae in Australia at a nested series of scales, both geographic and systematic. At the largest scale, the relationships between Australian and Indo-West Pacific species were inferred using the two most common atyid genera in Australia, Caridina and Paratya. Most atyids are hypothesised to have colonised Australia from Southeast Asia, but Paratya may be a Gondwanan relict given its distribution. Australian Paratya all form a strong clade, with a sister relationship to species from Tasman Sea islands. Molecular clock estimates place all of the splits within Paratya after the break-up of Gondwana, with Australia being colonised once 3½-8½ million years ago. This transoceanic dispersal is conjectured to have taken place through oceanic currents because of the amphidromous life cycle of some taxa of Paratya. Caridina has a very different biogeographic history in Australia, as numerous Australian species have close evolutionary relationships with non-Australian taxa from locations throughout the region. This implies many colonisations to or from Australia over a long period, and thus highlights the surprising adeptness of freshwater shrimp in dispersal across ocean barriers and the unity of much of the region's freshwater biota. A number of potential species radiations within Australia were also identified. This agrees with patterns detected for a large number of Australian freshwater taxa, and implies a vicariant explanation due to the development of colder, dryer climates. The systematic relationships of the remaining two Australian surface genera (Caridinides, Australatya) and two subterranean genera (Parisia, Pycnisia) were also investigated. Australatya forms a strong clade with Pacific 'Atya-like' genera, and Caridinides falls within a clade containing Australian Caridina. The hypogean genera, Parisia and Pycnisia, form a strong clade in all analyses, implying an Australian subterranean speciation. The possibility of a relationship between Parisia/Pycnisia and some Australian Caridina species may have implications for the monophyly of the highly disjunct genus Parisia, as it may descend from local Caridina species and represent convergent morphologies. The common and speciose genus Caridina was used as a model taxon for analyses within Australia. At the medium scale, molecular taxonomic techniques were used to uncover cryptic species within a problematic east Australian species complex. At least five species were detected. Phylogeographic and population genetic analyses were carried out on each of these five cryptic species, which diverged from each other in the late Miocene/Pliocene. There were very large differences between the species in the scales of overall geographic distribution, intraspecific divergence and population structure. These were characterised as either: 1) species with large ranges, low intraspecific divergence, limited phylogeographic structuring (Caridina sp. D); 2) species with large ranges, high intraspecific divergence, a high level of phylogeographic structuring (sp. B); 3) species with a limited range, low intraspecific divergence, no phylogeographic structuring (sp. E); or 4) species with limited ranges, high intraspecific divergences, a high level of phylogeographic structuring (sp. A & C). These patterns reflect a combination of large-scale factors, such as landscape structure and climate change, and small-scale factors, such as species-specific tolerances to local conditions and differing dispersal capabilities. Life history variation (egg size) between species may be correlated with different dispersal abilities. Species with the smallest eggs have the least intraspecific divergence and largest distribution, while those with the biggest eggs have the most divergence and smallest distribution, with medium-sized egg species in between. At the smallest phylogeographic scale, C. sp. C from the sand dune islands of Moreton Bay in southeastern Queensland was further analysed. Two different lineages (C1, C2) were found which diverged from each other during the late Miocene/Pliocene and so are older than the current landscape in which they are found. Small-scale phylogeographic analyses within C1, C2 and a sympatric fish identified divergences dating to the Pleistocene (about 100-300 thousand years ago). This implies that ice age sea-level changes may have structured these populations, although there is little observable influence of the last glacial maximum (about 18 thousand years ago). This study has highlighted a number of taxonomic anomalies within the Atyidae. The detection of many cryptic species implies that biodiversity within freshwater invertebrates is higher than currently appreciated. The evolutionary and biogeographic relationships of Australian atyids have proved complex, with many taxa having their own individual histories. At the large Indo-Pacific scale, dispersal is most evident, but within Australia, both vicariance and dispersal have been responsible for structuring all taxa at every scale.