A dissertation of published papers submitted by the author for the degree of Doctor in AgricSciences / Thesis (DSc)--Stellenbosch University, 1983. / ENGLISH ABSTRACT: No abstract available / AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar

The scarcity of feed resources often imposes a major challenge to the development of animal production in the tropics and subtropics. However, by-products have long been recognised in providing cheaper alternative feed ingredients relative to conventional feed ingredients, thus alleviating the challenge. The aim of the present study was to investigate the potential of three waste products from the subtropical fruit processing industry in animal feeding. The products include avocado meal (AM), macadamia oil cake (MOC) and macadamia chips (MCH). The samples were obtained from the processing plants in Nelspruit of Mpumalanga Province, Makhado (Louis Trichardt) and Tzaneen of Limpompo Province. Eight samples of each waste product were collected, prepared and their chemical composition, in vitro dry matter digestibility and, in situ ruminal dry matter and crude protein (CP) degradability were determined. A broiler growth trial was conducted as well to determine the effect of replacing maize with AM on performance of broilers under commercial production thus establishing the replacement value of avocado meal in broiler feeds. As the products under study were from the oil extraction process of the two fruits, there were high remnants of oil in all three waste products. As a result the ether extract (EE) content of the three waste products was higher compared to any of the commonly used oilseed meals. The MCH had the highest EE concentration, followed by the MOC and the AM had the lowest. However, after defatting the AM had the highest (P<0.05) EE concentration compared to either the MOC or MCH. The CP concentration of the MOC was significantly higher than that of the AM and MCH. There was no significant difference observed between the AM and MCH in CP concentration. A better amino acid profile was observed with the MOC compared to the AM and MCH. None of the products can be regarded as a protein source. The fibre fractions of the MCH were significantly higher than that of AM and MOC, with the exception of the acid detergent lignin (ADL) concentration. The ADL concentration of the AM and MCH did not differ significantly although the MCH concentration was higher. The MOC had the lowest (P<0.05) ADL concentration compared among these waste products. Generally, the fibre concentration in the MOC was lower compared to other waste products but higher compared to the SBM and POCM. The acid detergent insoluble nitrogen (ADIN) concentration of the AM was significantly higher than that of MOC and MCH. There was no significant difference observed between MOC and MCH. The condensed tannin (CT) concentration of the waste products differed significantly with the AM being the highest and the MOC the lowest. The ADIN concentration of the MOC could be compared to that of the SBM and lower than that of the POCM. There were some significant differences observed in the mineral composition between the waste products under study. The mineral concentrations were below the maximum tolerable levels of animals except for iron (Fe) in AM which can be toxic to sheep as it was above the maximum tolerable level of 500mg/kg. The in vitro dry matter digestibility of the three waste products differed significantly, with the MOC being the highest and the MCH the lowest. Huge variation within waste products was observed and it could be due to the contamination levels of the products with the indigestible portions of the parent fruits. The in situ degradability of dry matter and CP of the AM and MOC differed significantly, with the MOC surpassing the AM in most of the degradability characteristics. The potential degradable fraction (“b”) and the degradation rate of the b fraction (“c”) fractions of the AM and MOC did not differ significantly. The MCH had more indigestible particles of the kernel and as a result it could not be analysed statistically and was therefore omitted. The effect of replacing maize with AM at different inclusion rates led to decreased feed intake and the final mass of the broilers during the trial period. The feed intake of the broilers on commercial diet was significantly higher than that of the broilers on commercial diets with avocado meal, except for the inclusion rate of 10% AM. The final mass, the ADG and the FCE of the broilers on commercial diet were significantly higher compared to the broilers on diet with AM irrespective of the inclusion rate. No mortalities observed during the experimental period. Copyright / Dissertation (MSc(Agric))--University of Pretoria, 2012. / Animal and Wildlife Sciences / unrestricted

Orientador: Marcos David Ferreira / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agrícola / Made available in DSpace on 2018-08-22T12:55:03Z (GMT). No. of bitstreams: 1 Braga_CelsodeOliveira_D.pdf: 3406174 bytes, checksum: 113a8ddedc3815bc23a5a33c9e335c89 (MD5) Previous issue date: 2013 / Resumo: O Brasil desponta como grande produtor agrícola, líder em tecnologia de ponta para regiões tropicais e evoluiu da condição de importador para a de grande exportador de alimentos, sendo o quarto produtor mundial de frutas, atrás dos Estados Unidos, da China e da Índia (IBGE, 2010). Para que esta posição se mantenha é fundamental investir em pesquisa e em inovação tecnológica. Este estudo foi desenvolvido em uma unidade beneficiadora de laranja localizada na região de Aguaí, Estado de São Paulo. A laranja foi escolhida devido à sua importância para o agronegócio brasileiro. Um modelo que fornece um quadro avaliativo de uma unidade de beneficiamento (UB) com indicadores de qualidade, de boas práticas agrícolas, de impactos mecânicos e de exigências laborais foi desenvolvido. Desta forma, dois instrumentos inéditos foram criados: o IAQ ou Instrumento para Avaliação de Qualidade e o IABPA, Instrumento para Aplicação das Boas Práticas Agrícolas. A obtenção de indicadores de Qualidade e de BPA permite posicionar a UB em relação às demais UB nacionais e internacionais (ranking) e elaborar o planejamento estratégico para garantir melhorias e competitividade cada vez maior. Para avaliação de Impactos Mecânicos e de Exigências Laborais procedimentos convencionais foram utilizados. A esfera instrumentada foi empregada para avaliação de impactos mecânicos e o instrumento NASA TLX para avaliação das exigências laborais mentais e físicas. Para avaliação de exigências visuais, utilizou-se o instrumento QAV, Questionário de Avaliação Visual. Por intermédio dessas avaliações, podem-se determinar condições adequadas da tecnologia dos equipamentos, da organização do trabalho, do projeto e das condições ambientais do galpão. O conjunto de instrumentos desenvolvidos e utilizados nesta pesquisa constitui um modelo de avaliação e certificação de uma UB que permite um amplo diagnóstico de avaliação e de certificação, com o objetivo de introduzir melhorias e contribuir para garantia da competitividade da UB e da posição do país como grande exportador de alimentos. Este modelo possibilita conhecer o processo produtivo para introdução de melhorias da qualidade e da produtividade, preservando os aspectos ambientais, sociais, de segurança alimentar, de saúde e segurança dos trabalhadores, com sustentabilidade e se aplica a unidades de beneficiamento de outras frutas e hortaliças / Abstract: Brazil is emerging as a major agricultural producer, leader in cutting edge technology for tropical regions and evolving from importer to becoming a major exporter of food, currently being the fourth largest producer of fruit, behind the United States, China and India (IBGE, 2009). In order for this position to be maintained, investment in research and technological innovation is essential. This study was conducted in an orange processing plant in the region of Aguaí, Sao Paulo. The orange was chosen due to its importance for Brazilian agribusiness. A model that provides a framework of an evaluative processing unit (BU) with indicators of quality, good agricultural practices, mechanical impacts and labor requirements was developed. Thus, two new instruments were created: the IAQ or Instrument for Quality Assessment and IABPA, the Instrument for the Application of Good Agricultural Practices. Obtaining Quality indicators and BPA enables positioning this UB in relation to other national and international UB (ranking) and develop strategic planning to ensure improvements and increasing competitiveness. Conventional procedures were used to evaluate impacts of Mechanical and Industrial Requirements. The instrumented sphere was used for the evaluation of mechanical impacts and NASA TLX instrument for the assessment of mental and physical job demands. For evaluation of visual requirements, we used the instrument QAV, Visual Assessment Questionnaire. Through these reviews, one can determine appropriate conditions of technology equipment, work organization, design and environmental conditions of the shed. The set of tools developed and used in this research is a model of evaluation and certification of a UB that allows a broad diagnostic evaluation and certification, in order to make improvements and contribute to ensuring the competitiveness of UB and the country's position as major food exporter. This model enables the understanding and improving of the production process for improvements in quality and productivity, preserving the environmental, social, food security, health and safety aspects of workers, with sustainability and can also be applied to processing units of other fruits and vegetables / Doutorado / Planejamento e Desenvolvimento Rural Sustentável / Doutor em Engenharia Agrícola

Thesis (MBA (Business Management))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The objective of the study was to establish whether a small-scale biomass renewable energy project that uses waste fruit and pomace as the predominant feedstock could be a feasible clean development mechanism (CDM) project. The study was based on the solid waste streams of a pome fruit processor. Renewable energy technologies that convert biomass into a methane rich gas were evaluated. These included the various anaerobic digestion technologies that yield biogas and gasification technologies that yield syngas. Gasification was not found to be a feasible technology due to the moisture content requirement of less than 20% against that of the biomass of 70-80% and due to the low bulk density of the biomass of 250 kg/m3 versus the required minimum of 500kg/m3. The biogas could either be fired in a combined heat and power (CHP) unit or in a dual fired burner with heavy furnace oil (HFO). Feeding the thermal energy and electricity from the CHP unit back to the processor or supplying the electricity to the national electricity grid was considered. The plant would supply electricity to the national electricity grid as an Independent Power Producer (IPP). Eskom would be the renewable energy purchasing agent (REPA) that purchases the electricity at an anticipated R0.96/kWh under a power purchase agreement (PPA) in terms of the renewable energy feed-in tariff phase II (REFIT) guidelines. The anticipated revenue for electricity was six fold the coal offset cost of R0.16/kWh, while there was no demand for the heat energy after the processing season, thus firing biogas in a dual fired burner was not feasible. The most feasible technology was the anaerobic digestion of the biomass using a continuous stirred tank reactor (CSTR) process followed by a CHP unit that feeds the heat to the processing facility and the electricity into the national electricity grid. Waste fruit and wet pomace would be codigested with abattoir waste during the operating season. Dried pomace, abattoir waste, waste fruit and any other non-woody material from the surrounding farms and packhouses would be digested after the season to optimize the utilisation of the plant capacity. A capacity of 11 dry tons per day for the case study at a cost of R13 138 889 yielded an IRR of 15.2% and a net present value (NPV) of - R1 498 616 based on a discount rate of 18%, when excluding revenue from the sale of carbon emission reductions (CERs). Therefore, without the sale of CERs, this project would be rejected as a non-feasible investment. The project is eligible for CDM registration as a small-scale renewable energy project activity. No barriers were identified that would prevent the registration of this project activity as a CDM project in South Africa. The sale of CERs increased the NPV of the optimum solution to R156 483 and the IRR to 18.3%. Registering the project as a CDM project activity improved the feasibility of the project to the extent that it was marginally feasible. The fermentation and hydrolysis of the biomass into bioethanol was investigated as an alternative to the generation of heat and electricity. Although the technology is proven, no facilities were identified that use spoilt apples or apple pomace as the substrate. This increased the risk of the project and a discount rate of 24% was set when calculating the project NPV. This project activity yielded an NPV of - R1 296 057 when excluding CDM revenue. The feasibility improved to an NPV of - R263 507 and an IRR of 23.4% when taking the additional revenue from the sale of CERs into account. This project alternative was only marginally out of the money. With the development of newer technology, this alternative could prove to be more attractive in the future.

Thesis (Msc Food Sc (Food Science))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: Bacteria belonging to the genus Alicyclobacillus are thermo-acidophilic spore-formers that are able to spoil acidic food and beverage products through the production of guaiacol and other taint compounds, which causes a medicinal off-flavour and/or odour in the products. This thesis reports on the comparison of methods used for the isolation of species of Alicyclobacillus, as well as the growth behaviour and guaiacol production of different strains isolated from the South African fruit processing environment. Two methods for guaiacol detection were also evaluated and compared. Three isolation methods frequently used by South African fruit processors were compared with regards to their ability to isolate a strain of A. acidoterrestris from diluted peach juice concentrate. Method 1, the International Federation of Fruit Juice Producers (IFU) Method No. 12, makes use of spread plating onto Bacillus acidoterrestris (BAT) agar plates; Method 2 involves pour plating using acidified potato dextrose agar (PDA); and Method 3 makes use of membrane filtration and incubation of the membrane on K agar. The IFU Method No. 12 was the most effective method for the isolation of A. acidoterrestris, with a recovery of 75.97%. These results support the use of the IFU Method No. 12 as a standard international method for the isolation and detection of species of Alicyclobacillus. Seven strains of Alicyclobacillus, including the type strains A. acidoterrestris DSM 3922T and A. acidocaldarius DSM 446T and five strains isolated from a South African fruit processing plant, A. acidoterrestris FB2, FB14, FB32, FB38 and A. acidocaldarius FB19, were analysed based on their growth characteristics and guaiacol production under optimum conditions. Strains were inoculated into BAT medium at pH 4.00, supplemented with 100 mg.L-1 vanillin, and incubated at 45°C for 7 d. All the strains had similar growth patterns, with cell concentrations increasing rapidly from 0-24 h, followed by a stabilisation around maximum cell concentrations of 105-107 cfu.mL-1. Cell concentrations after heat shock, measured as an indication of spore formation, increased to maximum values of 105-107 cfu.mL-1, indicating an increase in spores as the cell density and competition for resources increased. All the strains were able to produce guaiacol in detectable concentrations [as measured by the peroxidase enzyme colourimetric assay (PECA)], and, therefore, possess the potential to cause product spoilage. Bacteria belonging to the genus Alicyclobacillus are thermo-acidophilic spore-formers that are able to spoil acidic food and beverage products through the production of guaiacol and other taint compounds, which causes a medicinal off-flavour and/or odour in the products. This thesis reports on the comparison of methods used for the isolation of species of Alicyclobacillus, as well as the growth behaviour and guaiacol production of different strains isolated from the South African fruit processing environment. Two methods for guaiacol detection were also evaluated and compared. Three isolation methods frequently used by South African fruit processors were compared with regards to their ability to isolate a strain of A. acidoterrestris from diluted peach juice concentrate. Method 1, the International Federation of Fruit Juice Producers (IFU) Method No. 12, makes use of spread plating onto Bacillus acidoterrestris (BAT) agar plates; Method 2 involves pour plating using acidified potato dextrose agar (PDA); and Method 3 makes use of membrane filtration and incubation of the membrane on K agar. The IFU Method No. 12 was the most effective method for the isolation of A. acidoterrestris, with a recovery of 75.97%. These results support the use of the IFU Method No. 12 as a standard international method for the isolation and detection of species of Alicyclobacillus. Seven strains of Alicyclobacillus, including the type strains A. acidoterrestris DSM 3922T and A. acidocaldarius DSM 446T and five strains isolated from a South African fruit processing plant, A. acidoterrestris FB2, FB14, FB32, FB38 and A. acidocaldarius FB19, were analysed based on their growth characteristics and guaiacol production under optimum conditions. Strains were inoculated into BAT medium at pH 4.00, supplemented with 100 mg.L-1 vanillin, and incubated at 45°C for 7 d. All the strains had similar growth patterns, with cell concentrations increasing rapidly from 0-24 h, followed by a stabilisation around maximum cell concentrations of 105-107 cfu.mL-1. Cell concentrations after heat shock, measured as an indication of spore formation, increased to maximum values of 105-107 cfu.mL-1, indicating an increase in spores as the cell density and competition for resources increased. All the strains were able to produce guaiacol in detectable concentrations [as measured by the peroxidase enzyme colourimetric assay (PECA)], and, therefore, possess the potential to cause product spoilage. iv The influence of temperature on the growth and guaiacol production of the Alicyclobacillus strains was also investigated and two guaiacol detection methods, the PECA and headspace gas-chromatography mass-spectrometry (HS GC-MS), were compared with regards to their ability to detect guaiacol. The strains were incubated at 25°C and 45°C for 6 d and samples analysed every 24 h. Growth of the A. acidoterrestris strains was slower at 25°C, and maximum cell concentrations were lower than at 45°C. A decrease in cell concentrations was observed in the A. acidocaldarius strains at 25°C, as this temperature is below their growth temperature range. All the strains were able to produce guaiacol at 45°C, with guaiacol only being detected once a cell concentration of 104-105 cfu.mL-1 had been reached. The maximum guaiacol concentrations detected at 45°C in the samples containing A. acidoterrestris were significantly higher than those detected in the A. acidocaldarius samples. At 25°C there was a longer lag phase before guaiacol was detected in the A. acidoterrestris samples, while no guaiacol was detected in the samples containing A. acidocaldarius. Because guaiacol is produced at ambient temperatures, cooling of products is recommended to control spoilage by A. acidoterrestris. The sensitivity of the two guaiacol detection methods also differed significantly and, therefore, the PECA is recommended for presence/absence detection of guaiacol, while HS GCMS is recommended where accurate quantification of guaiacol is required. Alicyclobacillus acidoterrestris FB2 was investigated for its ability to grow and produce guaiacol in white grape juice supplemented with vanillin at different concentrations. Alicyclobacillus acidoterrestris FB2 was inoculated into white grape juice concentrate diluted 1:10 with distilled water containing 0-500 mg.L-1 vanillin and incubated at 45°C for 6 d. Similar growth patterns were observed in all the samples, except in the sample containing 500 mg.L-1 vanillin, which had a longer lag phase of growth. Guaiacol concentrations, detected using the PECA, increased as the vanillin concentration increased, with the exception of the sample containing 500 mg.L-1 vanillin, where less guaiacol was detected than in the sample containing 250 mg.L-1 vanillin, due to growth inhibition caused by the higher vanillin concentration. A number of conditions need to be favourable for detectable guaiacol production to occur and it could, therefore, be possible to minimise or prevent guaiacol production by controlling or eliminating some of these factors. Good manufacturing practices should be employed in order to minimise contamination and, therefore, spoilage, by Alicyclobacillus species. / AFRIKAANSE OPSOMMING: Bakterieë wat aan die genus Alicyclobacillus behoort, is termo-asidofiliese spoorvormers wat suur voedsel en drank produkte kan bederf deur die produksie van guaiakol en ander bederf verbindings, wat ‘n medisinale geur en/of reuk in die produkte veroorsaak. Hierdie tesis doen verslag oor die vergelyking van metodes wat vir die isolasie van spesies van Alicyclobacillus gebruik word, sowel as die groei kenmerke en guaiakol produksie van verskillende stamme wat uit die Suid- Afrikaanse vrugte prosesseringsomgewing geïsoleer is. Twee metodes vir die deteksie van guaiakol is ook geëvalueer en vergelyk. Drie isolasie metodes wat algemeen deur Suid-Afrikaanse vrugteprosesseerders gebruik word, is vergelyk ten opsigte van hul vermoë om H A. acidoterrestris stam uit verdunde perskesap konsentraat te isoleer. Metode 1, die Internasionale Federasie van Vrugtesap Produseerders (IFU) Metode No. 12, maak gebruik van spreiplating op Bacillus acidoterrestris (BAT) agar plate; Metode 2 behels gietplating met aartappel dekstrose agar (PDA) and Metode 3 maak gebruik van membraan filtrasie en inkubasie van die membraan op K agar. Die IFU Metode No. 12 was die mees effektiewe metode vir die isolasie van A. acidoterrestris, met H sel herwinning van 75.97%. Hierdie resultate ondersteun die gebruik van die IFU Metode No. 12 as H standaard internasionale metode vir die isolasie en deteksie van spesies van Alicyclobacillus. Sewe Alicyclobacillus stamme, insluitende die tipe stamme A. acidoterrestris DSM 3922T en A. acidocaldarius DSM 446T en vyf stamme geïsoleer uit ‘n Suid- Afrikaanse vrugte prosesseringsaanleg, A. acidoterrestris FB2, FB14, FB32, FB38 en A. acidocaldarius FB19, is geanaliseer met betrekking tot hul groei kenmerke en guaiakol produksie onder optimum toestande. Stamme is in BAT medium by pH 4.00, aangevul met 100 mg.L-1 vanillin, geïnokuleer en geïnkubeer teen 45°C vir 7 d. Al die stamme het soortgelyke groeipatrone getoon, met selgetalle wat vinnig toegeneem het van 0-24 h, gevolg deur ‘n stabilisering rondom maksimum selgetalle van 105-107 kve.mL-1. Selgetalle na hitte behandeling, gemeet as H aanduiding van spoorvorming, het toegeneem tot maksimum waardes van 105-107 kve.mL-1, wat aandui dat spore toegeneem het soos die seldigtheid en kompetisie vir voedingsbronne toegeneem het. Al die stamme kon guaiakol in bespeurbare konsentrasies produseer [soos gemeet deur die peroksidase ensiem kolorimetriese bepaling (PEKB)] en besit dus die potensiaal om produkte te bederf. Die invloed van temperatuur op groei en guaiakol produksie van die Alicyclobacillus stamme is ook ondersoek en twee guaiakol deteksie metodes, die PEKB en topspasie gas-kromatografie massa-spektrometrie (TS GK-MS) is vergelyk ten opsigte van hul vermoë om guaiakol op te spoor. Die stamme is geïnkubeer teen 25°C en 45°C vir 6 d en monsters is elke 24 h geanaliseer. Groei van die A. acidoterrestris stamme was stadiger by 25°C en maksimum selgetalle was laer as by 45°C. H Vermindering in selgetalle is waargeneem in die A. acidocaldarius stamme by 25°C, aangesien hierdie temperatuur buite hul groei temperatuur grense val. Al die stamme kon guaiakol produseer by 45°C, met guaiakol deteksie wat eers H aanvang geneem het nadat H sel konsentrasie van 104-105 kve.mL-1 bereik is. Die maksimum guaiakol konsentrasies wat by 45°C in die monsters met A. acidoterrestris opgespoor is, was beduidend hoër as die konsentrasies wat in die A. acidocaldarius monsters opgespoor is. By 25°C was daar H langer sloerfase voor guaiakol opgespoor is in die A. acidoterrestris monsters, terwyl geen guaiakol opgespoor is in die monsters wat A. acidocaldarius bevat het nie. Aangesien guaiakol by kamertemperatuur geproduseer word, word verkoeling van produkte aanbeveel ten einde bederf deur A. acidoterrestris te beheer. Die sensitiwiteit van die twee guaiakol deteksie metodes het ook beduidend verskil en dus word die gebruik van die PEKB aanbeveel vir teenwoordigheid/afwesigheid deteksie van guaiakol, terwyl TS GK-MS aanbeveel word waar akkurate kwantifisering van guaiakol vereis word. Ondersoek is ingestel na die vermoë van A. acidoterrestris FB2 om te groei en guaiakol te produseer in witdruiwesap aangevul met verskillende vanillin konsentrasies. Alicyclobacillus acidoterrestris FB2 is geïnokuleer in witdruiwesap konsentraat 1:10 verdun met gedistilleerde water wat 0-500 mg.L-1 vanillin bevat het en is geïnkubeer teen 45°C vir 6 d. Soortgelyke groeipatrone is waargeneem in al die monsters, behalwe die monster wat 500 mg.L-1 vanillin bevat het, wat H langer sloerfase van groei gehad het. Guaiakol konsentrasies, soos gemeet deur die PEKB, het toegeneem soos die vanillin konsentrasie toegeneem het, met die uitsondering van die monster wat 500 mg.L-1 vanillin bevat het, waar minder guaiakol opgespoor is as in die monster wat 250 mg.L-1 bevat het as gevolg van groei inhibisie veroorsaak deur die hoër vanillin konsentrasie. H Aantal toestande moet gunstig wees vir guaiakol produksie om plaas te vind en dit kan dus moontlik wees om guaiakol produksie te minimaliseer of te voorkom deur die beheer of uitskakeling van sommige van hierdie faktore. Goeie vervaardigingspraktyke moet in plek gestel word ten einde kontaminasie en bederf deur Alicyclobacillus spesies tot H minimum te beperk.

<p>Sporedni proizvodi industrijske prerade voća, trop borovnice i maline, postupcima su&scaron;enja i mlevenja prevedeni su u oblik pogodan za aplikaciju u prehrambene proizvode. Ovako dobijeni sastojci imaju visok sadržaj prehrambenih vlakana, esencijalnih masnih kiselina poput linolne i &alpha;-linolenske, a karakteri&scaron;e ih i izbalansiran odnos PUFA/SFA. Od makronutrijenata, u najvećoj koncentraciji prisutni su K, Mg i Ca. Imajući u vidu da su glavne fitohemikalije bobičastog voća polifenolna jediinjenja, u proizvedenim funkcionalnim sastojcima određen je sadržaj ukupnih rastvorljivih polifenola, antocijana i flavonoida, kao i sastav i sadržaj odabranih polifenolnih jedinjenja primenom LC/MS-MS tehnike.<br />Primena osu&scaron;enih i samlevenih tropova kao sastojaka prehrambenih proizvoda ispitana je u formulaciji bezglutenskog keksa, sa ciljem njegovog funkcionalnog obogaćivanja. Za optimizaciju formulacije keksa kori&scaron;ćen je postupak odzivne povr&scaron;ine (RSM), a formulacija u kojoj je bezglutenska sme&scaron;a zamenjena sa 28,2% tropa borovnice i 1,8% tropa maline dobijena je kao optimalna, uzimajući u obzir sve zadate kriterijume. Dodatkom ova dva funkcionalana sastojka u gorenavedenom odnosu, postignuto je značajno pobolj&scaron;anje nutritivnih svojstava bezglutenskog keksa, a dobijeni proizvod po kvalitetu može da parira komercijalno dostupnim funkcionalno obogaćenim keksovima, namenjenim zdravoj populaciji.<br />Rezultati dijetetske interventne studije sprovedene na 20 zdravih, normalno uhranjenih ispitanika ženskog pola, ukazuju da se konzumiranjem kreiranog bezglutenskog keksa u količini od 32 g dnevno, postiže značajna redukcije LDL holesterola, i povećanje nivoa adiponektina u krvi, a budući da se ovaj adipocitni-sekretorni protein dovodi u vezu sa antiinflamatornim i antiaterogenim potencijalom u tretmanu metaboličkog sindroma i dijabetesa tipa 2, može se očekivati protektivno dejstvo kreiranog bezgutenskog keksa.</p> / <p>Blueberry and raspberry pomace, by-products from fruit juice industry, were processed into the new food ingredients by drying and grinding. The obtained functional ingredients are characterized by high content of dietary fibres and essential fatty acids (linoleic and &alpha;-linolenic acid), as well as by optimal ratio of PUFA/SFA. The main macronutrients in dried and ground pomace are K, Mg and Ca. The content of total phenolic compounds, anthocyanins and flavonoids were determined, as well as composition and content of individual phenolic compounds using LC/MS-MS in order to evaluate their antioxidant potential.<br />In the form of dried powder, blueberry and raspberry pomace were used as ingredients in formulation of gluten-free cookies. Response surface methodology (RSM) approach was applied to obtain optimal ingredients proportion. Formulation in which gluten-free flour mixture is substituted with 28.2% of the blueberry and 1.8% of the raspberry pomace was found to be optimal following the used optimization criteria. Addition of blueberry and raspberry pomace has led to a remarkable improvement in nutritional properties, and resulted in a product with similar nutritional profile with commercially available gluten containing cookies.<br />Dietary intervention study on a group of 20 healthy, normal weight female subjects was conducted to investigate the health effect of the created gluten-free cookies. The results showed a significant reduction in levels of LDL cholesterol, and an increase in plasma concentrations of adiponectin, suggesting a potential positive effect of the created product on human health.</p>

Thesis (MSc Food Sc )--Stellenbosch University, 2002. / ENGLISH ABSTRACT: South Africa is a developing country that relies heavily on its agricultural sector for economical welfare especially in the Western Cape Province. However, development gives rise to new technologies, new products, economical stability and unfortunately also to the production of larger volumes of liquid and solid waste. Anaerobic composting is becoming a very attractive treatment option for solid waste disposal because of its unique operational advantages and two valueadded by-products, compost and biogas. Over the last decade progress has been made in anaerobic digestion of solid wastes, but no literature could be found on the anaerobic composting of apple and peach pomace. The main objective of this study was to develop a method to anaerobically compost apple and peach pomace. In the first phase important operational parameters were identified and a method was developed to optimise the parameters. In the second phase of the study, the scaling-up and optimisation of the process were the major objectives. During the first phase of this research 2 L modified glass containers were used as composting units. The most important operational parameters (leachate pH, inoculum source and size, and initial moisture levels) were identified. Anaerobic compost from previous tests, brewery granules and anaerobic sludge were also used as inocula and evaluated for the best source of microbes. After optimising all the identified parameters, good results were obtained, which included higher biogas production, good volume reductions, less bad aromas and a compost product with a neutral pH. After developing the 2 L laboratory-scale method to compost the apple pomace anaerobically, the next step was to ascertain if the method would work if larger volumes of solid fruit waste were composted. A special 20 L composting unit made of PVC was designed to suit the operational requirements of the anaerobic composting process. It was also decided to mix apple pomace and peach pulp together and to use this solid waste source as part of the composting substrate. Different inocula, including cattle manure, anaerobic sludge, brewery granules and anaerobic compost produced in the previous tests, were used. Although good results were obtained with the anaerobic compost and cattle manure as inoculum, the aim was also to decrease the composting period by shortening the pH stabilisation period. To achieve this, it was decided to add NaHC03 to the substrate to be composted to facilitate a faster pH stabilisation. The composting period was subsequently shortened to 25 days with satisfactory results, which included a volume reduction, biogas production and faster pH stabilisation. An upflow anaerobic sludge blanket (UASB) bioreactor was also used to assist the composting process by facilitating the removal of the VFA's present in the composting leachate. This proved to be a valuable addition to the composting process as the UASB bioreactor also provided the composting units with a 'moisturising liquid', which was 'enriched' with a consortium of active anaerobic bacteria when the effluent from the bioreactor was re-added to the composting units. With all the operational parameters in place, good results were obtained and these included a volume reduction of 60% (m/m), a good biogas production, a composting period of only 25 days, a compost that was free of bad aromas, a final compost pH of > 6.5, final leachate COD values of less than 3 000 rnq.l", and a final leachate VFA's concentration of between 0 and 250 rnq.l". If in future research further scaling-up is to be considered, it is recommended that the composting unit be coupled directly to the UASB bioreactor, thus making the process continuous and more practical to operate. If the operational period of the anaerobic composting set-up could be further shortened and the inoculum adapted so that the process could be used for the treatment of other difficult types of solid wastes, it would probably be advantageous for the fruit processing industry to use this method as an environmental control technology. / AFRIKAANSE OPSOMMING: Suid-Afrika is 'n ontwikkelende land wat baie afhanklik is van die sukses van die landbousektor vir ekonomiese welstand, veral in die Wes Kaap Provinsie. Ontwikkeling gaan gepaard met nuwe tegnologie, nuwe produkte, ekonomiese stabiliteit en daarmee saam gaan die produksie van groter volumes vlooiebare en soliede afvalprodukte. Anaërobiese kompostering is tans besig om opgang te maak as en doeltreffende behandelingstegnologie vir vaste afvalstowwe. Tydens die laaste dekade is baie vooruitgang gemaak in die veld van anaërobiese vertering asook kompostering van afvalmateriaal met en hoë vaste stof inhoud. Anaërobiese kompostering van appel- en perskepulp, afkomstig van die versappingsindustrie, het tot dusver min aandag geniet. Die hoofdoel van hierdie navorsing was om 'n anaërobiese komposterings metode te ontwikkel vir die beheer van vrugte afval om sodoende die basis neer te lê vir en nuwe tegnologie wat baie voordele (biogas en kompos) inhou. In die eerste fase is die belangrikste operationele parameters geïdentifiseer om sodoende beter beheer oor die anaërobiese proses uit te oefen. In die tweede fase is die anaërobiese proses wat gedurende die eerste fase ontwikkel is, opgeskaal om optimum resultate te verkry. Gedurende die eerste fase van hierdie verhandeling was 2 L gemodifiseerde glas houers gebruik as komposteringseenhede. Die belangrikste operasionele parameters (pH beheer, inokulasie grootte, vloeistofvlakke en hoeveelheid vog asook vlugtige vetsuur produksie en verwydering) vir die beheer van die anaërobiese komposteringsproses was geïdentifiseer en gebruik as uitgangspunt om 'n anaërobiese komposteringsmetode te ontwikkel. Anaërobiese slyk, brouery granules en anaërobiese kompos van vorige eksperimente was as inokula gebruik. Gedurende hierdie studies was goeie resultate verkry en het 'n hoë biogas produksie, goeie volume reduksies, vermindering van slegte aromas en kompos met 'n neutrale pH ingesluit. . Nadat hierdie goeie resultate met die 2 L laboratorium-skaal metode verkry was, was groter volumes vaste vrugte afval gebruik om te bepaal of dieselfde metode toegepas kan word op en groter skaal. Spesiale 20 L komposteringseenhede was ontwerp om aan die operasionele vereistes van 'n anaërobiese proses te voldoen. Dit was ook besluit om appel pulp met perske pulp te meng en te gebruik as deel van die komposteringssubstraat. Verskeie inokula was weereens gebruik en het die volgende ingesluit: vars beesmis, anaërobiese slyk, brouery granules en anaërobiese kompos van vorige eksperimente. Hoewel baie goeie resultate met vars beesmis en anaërobiese kompos as inokula verkry was, was 'n volgende doel gewees om die kompoterings tydperk te verkort deur die pH vinniger te stabiliseer. Daar was besluit om NaHC03 by die komposteringssubstraat te voeg en so 'n vinniger pH stabilisasie te fasiliteer. 'n UASB ('upflow anaerobic sludge blanket') bioreaktor was ook gebruik om die komposteringsproses aan te help deur die vlugtige vetsure wat in die kompostloog teenwoordig was, te verwyder. Die insluiting van die bioreaktor in die anaërobiese komposteringsproses het bygedra tot die sukses van die proses deurdat die uitvloeisel as 'n vogmiddel vir die komposteringseenhede gebruik was en 'n konsortium van aktiewe anaërobiese bakterieë bevat het. Nadat al die operationele parameters in plek was, was goeie resultate bereik en het die volgende ingesluit: 'n volume reduksie van 60% (m/m), goeie biogas produksie, 'n komposteringstyd van 25 dae, 'n kompos wat vry was van slegste aromas, 'n finale kompos pH van >6.5, finale loog CSB van <3 000 rnq.l' en 'n finale vetsuur konsentrasie van tussen 0 en 250 mq.l'. lndien verdere navorsing onderneem word, word dit aanbeveel dat die UASB bioreaktor direk aan die komposteringseenheid gekoppel word om sodoende die proses meer aaneenlopend en die proses prakties makliker uitvoerbaar te maak. Indien die operationele tydperk nog korter gemaak kan word en die inokulum aanpasbaar kan wees om moeilik verteerbare afvalprodukte te akkomodeer, sal hierdie tegnologie baie voordelig wees as 'n metode om omgewingsbesoedeling te beheer

The main aim of my thesis is to create sustainable local food production in Jaroslavice region. This region is situated near the Austria – Czechia border. The political changes of previous century influenced this rural location a lot. Changes resulted in socioeconomic degradation of this area. My project tends to shorten production connections between producers and consumers. It also takes the part with local farmers and farmer´s market. My architecture proposal is a building of regional food center focused on fruit production. The main fruits are apples, apricots and pitches according to the local climate conditions. The center has three main parts – processing, storage and retail.