<p>The purpose of this thesis is to analyse the Swedish debate regarding genetically modified food products. What does the moral disagreements within this debate entail? In which way would it be possible to obtain and maintain a true dialogue? Is consensus a plausible or even a desirable goal? Can communicative ethics contribute? These questions are analysed in this research project.</p><p>The project focuses on three themes of the debate on genetically modified food 1990-2000 in Sweden. The first theme is in relation to the concepts: natural and unnatural. Within the debate on genetically modified food, there have been various wide ranging arguments on whether or not genetic modification is natural or unnatural. </p><p>The second theme concerns the concepts of risk and benefit. Safety questions and possible risks and benefits have been subjects of discussion and debate for a long time. This analysis highlights the way risks or benefits are valued. What does it mean if something is a risk? </p><p>The third theme concerns the argumentations regarding democracy, mostlyin relation to labelling discussions. Democracy is used to describe freedom of choice, another argumentation focuses on the right to be a part of the decision making process.</p><p>The last part of the thesis discusses the question whether communicative ethics can contribute to obtain dialogue between various parties, and whether consensus is possible or even a desirable as a goal. How would this function in a debate where there is an ethical disagreement based on divergent ethical standpoints? </p>

The purpose of this thesis is to analyse the Swedish debate regarding genetically modified food products. What does the moral disagreements within this debate entail? In which way would it be possible to obtain and maintain a true dialogue? Is consensus a plausible or even a desirable goal? Can communicative ethics contribute? These questions are analysed in this research project. The project focuses on three themes of the debate on genetically modified food 1990-2000 in Sweden. The first theme is in relation to the concepts: natural and unnatural. Within the debate on genetically modified food, there have been various wide ranging arguments on whether or not genetic modification is natural or unnatural. The second theme concerns the concepts of risk and benefit. Safety questions and possible risks and benefits have been subjects of discussion and debate for a long time. This analysis highlights the way risks or benefits are valued. What does it mean if something is a risk? The third theme concerns the argumentations regarding democracy, mostlyin relation to labelling discussions. Democracy is used to describe freedom of choice, another argumentation focuses on the right to be a part of the decision making process. The last part of the thesis discusses the question whether communicative ethics can contribute to obtain dialogue between various parties, and whether consensus is possible or even a desirable as a goal. How would this function in a debate where there is an ethical disagreement based on divergent ethical standpoints?

The aim was to study consumer attitudes to and perceptions of organic and genetically modified (GM) foods and factors influencing the purchase/non purchase of these two types of foods. Data were collected by two questionnaire surveys (random nation-wide samples of 2000 consumers, response rate 58% and 39% respectively) and one interview study (n=40). A majority of the consumers had positive attitudes towards purchasing organic foods but few reported purchasing organic foods regularly. The most common beliefs about organic foods were that they are more expensive and healthier. Choice of organic foods was related to the perceived consequences for human health and to environmentally friendly behaviour. Perceived positive health consequences appeared to be a stronger motive for purchasing organic foods than were environmental benefits. Attitudes towards genetic engineering (GE) appear to be dependent on the application area. Medical applications were generally more easily accepted than were food and agricultural applications. However, all medical applications were not equally accepted. Consumers had generally rather negative attitudes towards GM foods, which were perceived as not being healthy. GM of animals for food production was perceived more negative than GM of plants. Tangible benefits of GM foods, like being better for the environment or healthier, seemed to have a potential to increase willingness to buy. The results indicate that the attitudes to organic and GM foods differ: the attitude to organic foods is positive while the attitude to GM foods is more negative. Further, the perceptions of organic and GM foods are opposed in relation to health. The purchase of organic foods and the potential willingness to purchase GM foods appear to be related to positive consequences for health and the environment.

This study examines the possible incompatibility between environmental conventions and agreements promoting international trade. More specifically, it aims at discussing the Precautionary Principle and the WTO agreements on trade and how they might contradict each other on the international arena. The study focuses on the international trade with genetically modified organisms and discusses probable needs of reformulating the principles and/or conventions concerned if these are to function as tools of equal importance when handling environmental problems through policy making. The controversy is illustrated through a case-study of a trade dispute where the conflicting principles: the Precautionary Principle and WTO agreements on trade are involved, namely a dispute where a formal complaint to the WTO by the US, Canada and Argentina about the European Community supposedly applying a de facto moratorium on genetically modified organisms had the final outcome of the European Community being found to contravene international trade rules. The study concludes that actors on the international arena are still opinions apart concerning the way to perceive possible risks connected to biotechnology and genetically modified organisms. This incongruence is also found to apply to the status of the Precautionary Principle as a rule of international law as well as to which of the conventions that were applicable to the concerned dispute.

A great effort has been recently devoted to the development of new devices for the detection of specific sequences of DNA, due to increasing need of label - free, fast, cheap, and miniaturized analytical systems able to detect target sequences for screening purposes, especially in food industry for genetically modified organisms (GMOs). In this study, development of a QCM - based DNA biosensor for the detection of the hybridisation of CaMV 35S promoter sequence (P35S) was investigated. Attention was focused on the choice of the coating chemistry that could be used for the immobilisation of probe sequences on the gold surface of the quartz crystal. Two immobilisation procedures were tested and compared considering the amount of the immobilised probe, the extent of the hybridisation reaction, the possibility of regeneration and the absence of non - specific adsorption. The two coating methods were based on the use of self - assembled monolayers. One of them employed the interaction between the thiol and gold for the immobilisation of a thiolated P35S probe, while the other employed formation of functionalised aldehyde groups by ethylenediamine plasma polymerization on the gold surface for the immobilisation of amined P35S probes through gluteraldehyde activation. Results indicated that immobilisation of a thiolated probe provides better immobilisation characteristic, higher sensitivity for the detection of the hybridisation reaction, absence of non - specific adsorption and a higher stability with respect to the regeneration step. The optimised immobilisation procedure for the thiolated probe was used for the detection of P35S sequence in PCR - amplified DNAs and in real samples of pflp - gene inserted tobacco plants that produce ferrodoxin like protein additionally. Fragmentation of the genomic DNAs were achieved by digestion with restriction endonucleases and sonication. The obtained results from the fragmented genomic DNAs demonstrated that it is possible to detect the target sequence directly in non-amplified genomic DNAs by using the developed QCM - based DNA biosensor system. The developed QCM-based DNA biosensor represented promising results for a real-time, label - free, direct detection of DNA samples for the screening of GMOs.

This study was carried out to analyze tomato samples and tomato seeds, purchased from different food markets of Turkey randomly, for the presence of genetic modification by using PCR method as it allows more specific detection. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and also with extraction kits. Screening tests of tomatoes were done by targeting 35S promoter, NOS terminator and NptII kanamycin resistance gene with eight different primer sets. Real time PCR is used to confirm 35S and NOS positives results obtained from conventional PCR. In this study, it was observed that 14 out of 35 seed samples, and 14 out of 40 fresh tomato samples which were screened had at least one transgenic element of 35S promoter, NOS terminator and NPTII kanamycin resistance gene indicating the possible presence of genetic modifications. After screening, gene specific studies were carried out for PG, sam-k indicating F type ripening delayed tomato and the 35 1 N lines respectively and cry1Ac genes inserted in 5345-1 insect resistant tomato line. PG and sam-k specific primers were not amplified in any of the samples investigated whereas 18 out of 75 samples were cry1Ac positive and 1 out of 75 samples was sam-k positive. Positives were confirmed by sequence analysis. Additionally, construct specific primers specific to 5345-1 and 35 1 N lines were designed. PCR amplicons indicate the existence of the construct sequence. In order to verify the results, PCR products were sent to sequence analysis

D.Tech. Business Administration. Business School. / Focuses on the awareness and knowledge of consumers on organically produced and genetically modified food, and how their self-rated level of knowledge impacts on their attitudes, perceptions and purchasing behaviour in the field. Furthermore, it highlights changes in consumer knowledge, perceptions, attitudes and purchasing behaviour over time, indicating to the rate of acceptance of these foods. In addition, it also supplies guidelines for future marketing communication on such food.

As of 2011 a revolutionary ‘movement of movements’ is emerging coterminous with environmental crises and various other crises including corporate globalization. This study sheds theoretical and empirical light on the origins of the movement of movements. Employing gendered, ethnicized class analysis, this study investigates Karl Marx’s (1867) central discovery in Capital volume one, chapter 32 that corporate concentration and organization impels workers to resist and become a revolutionary class for themselves. Data is derived from investigation into the social movement La Via Campesina’s (‘the peasant way’) struggle against Monsanto Corporation in India, the European Union and Brazil during two periods of Monsanto’s concentration (1996–1998 and 2007–2011). Findings indicate that, in the process of Monsanto’s concentration, there was a leap forward in the formation and actions of the movement of movements. This study concludes that corporate concentration and global organization significantly impels the formation of the movement of movements. / Social Science and Humanities Research Council (SSHRC)

Healthy term pregnancy requires precisely timed coordination of multiple systems, including reproductive, neuroendocrine, immune and cardiovascular. Dynamic maternal alterations occur systemically as well as locally within the reproductive tract. Systemic cardiovascular changes during gestation are relatively conserved in mammals, permitting comparison. These physiological changes are relatively acute and reversible, in contrast to the pathological changes seen during cardiovascular disease development. Gestational hypertensive disorders, such as preeclampsia, are the leading causes of maternal and fetal morbidity and mortality. The pathogenesis of preeclampsia is not fully elucidated, but perturbation of the immune system is a fundamental component. The angiogenic and vascular properties of uterine NK lymphocytes have been well studied in mice and women, but their relationships to gestational blood pressure regulation and cardiovascular adaptations have not been addressed. In non-pregnant women and mice, T cells, but not B cells, have been found to alter cardiovascular functioning. NK cells in humans also possess these capabilities, but no functional studies have been completed. The aim of this thesis was to define the role of NK and T lymphocytes in cardiovascular adaptations during mouse gestation. Using chronic radiotelemetry, histology, post-mortem and other techniques, female inbred mice of differing genotypes that lack specific lymphocyte subsets were compared before and across gestation. In normal, immune competent mice, a five-phase gestational blood pressure profile was found. This dynamic profile corresponded to stages of placental development. In mice with a compound deficit in arterial modification and lymphocytes, no gestational hypertension was observed. To elevate the maternal challenge of pregnancy, studies of pregnant, autoimmune Type 1 Diabetic mice were conducted. Impaired spiral artery remodeling, dysfunctional lymphocytes and growth-restricted fetuses were identified. From mid-gestation, diabetic pregnant mice were hypotensive and bradycardic and showed signs of pre-renal failure (proteinuria and electrolyte imbalances). In pregnant mice lacking T cells, tachycardia was observed despite otherwise normal gestational outcomes. In pregnant mice lacking T cells with impaired NK cells, blood pressure was blunted and tachycardia was observed. These findings support the conclusion that impaired spiral artery remodeling is insufficient to cause gestational hypertension in mice. The data further identify a role for T and NK cells in cardiac function during gestation. / Thesis (Ph.D, Anatomy & Cell Biology) -- Queen's University, 2010-09-01 20:56:15.648

Thesis (D. Tech.: Biotechnology)-Dept. of Biotechnology, Durban University of Technology, 2006 xii, 274 leaves / In Sub-Saharan Africa there is an urgent need to sustain and improve the quality of its food resources. Poverty eradication features high on the agenda of a number of world health organisations, while the number of underweight children in Africa continues to increase (Pellet, 1996). Providing nutritionally enhanced foods to the poor will help towards achieving this objective. Protein-energy malnutrition has been identified as one of the most important problems facing Africa, with maize as the staple diet (Nkama et al., 1995). However, a combination of several factors limits availability and the nutritional quality of maize. During starvation, energy and protein intakes decrease by 20-30%, with most of the children in Africa having an average protein intake of only 20 g per day (Igbedioh, 1996). Energy availability also affects protein utilization because of interrelationships of protein and energy metabolism (Elwyn, 1993). The diets of inhabitants in developing regions depend mainly on cereals (maize) for both protein and dietary energy which lacks indispensable amino acids, minerals, vitamins and carbohydrates. In light of these growing concerns an attempt was made to devise a scientific strategy to combat the nutritional shortfalls of maize meal. A multidisciplinary and concerted approach was followed within this project aimed at designing an improved thermostable amylase and applying the enzyme to nutritionally enhance maize meal. It was envisaged that the manipulation of maize meal, by the application of enzyme technology will improve the nutritional status of this staple food. The consequences is that an alternate solution for the eradication of an ailing, poverty stricken and malnourished African population is achievable. It is possible that the boundaries defining the limits of life will extend to even greater extremes through the application of novel technologies.