M. Tech. / OBJECTIVE: To determine the most effective treatment protocol in the treatment of Upper Crossed Syndrome by comparing objective results gained from Spinal Manipulative Therapy and a stretching program only and a combination of these treatments directed at the shoulder girdle and cervical spine. DESIGN: The study was a clinical trial in which three experimental groups of fifteen participants each were compared to each other. These participants were recruited from the local general population and were selected on the basis of inclusion and exclusion criteria, presenting with Upper Crossed Syndrome and demonstrating unremarkable clinical and radiological findings.

No / This study aimed to address whether a water exercise programme improves pain and quality of life in pregnant patients with Pelvic Girdle Pain (PGP) compared to a land-based exercise programme and the feasibility of undertaking a large-scale research programme. Twenty-three participants with diagnosed PGP, recruited at St George’s Hospital London, were randomised into two groups (water or land exercise). Each group received, four, once-weekly exercise sessions on land or water. Exercise effects on PGP were measured using the Pelvic Girdle Pain Questionnaire (PGPQ) (primary outcome), Visual Analogue Scale (VAS), Patient Specific Functional Score (PSFS) and Active Straight Leg Raise (ASLR). Quality of life was measured using the Subjective Exercise Experience Scale (SEES). Outcomes were assessed at baseline and post four weeks exercise. Results showed there was a clinically significant improvement shown in all outcome measures in the water group, compared to the land group. A statistical difference between groups was shown for ASLR (p=0.036), Positive Well-Being (p=0.000) and Fatigue levels (p=0.011). No statistical difference was shown for PGPQ (p=0.056), PSFS (p=0.530) and Psychological Distress (p=0.712) scores. Exercise in water appears to offer a clinical benefit for patients experiencing PGP compared to a land exercise, particularly with Fatigue, Positive Well-Being and ASLR scores. Statistical differences between groups are limited by small sample size and that no power calculation was used in this study. Methodology and results provide support for a larger study on this topic to provide more definitive conclusions to support the use of water-based therapy for PGP.

Introdução: As distrofias musculares de cinturas (limb-girdle muscular dystrophies - LGMD) são causadas por mutações em uma grande variedade de genes que codificam proteínas musculares, podendo ser herdadas de forma autossômica dominante ou recessiva. O diagnóstico é feito tanto através de exame de biópsia muscular que mostra um padrão histológico distrófico ao lado de deficiência específica de proteínas musculares quanto por estudo genético. Em alguns subtipos de LGMD não é possível fazer o diagnóstico específico pela biópsia muscular, tais como na deficiência da calpaína-3 (CAPN3) e da proteína relacionada a fukutina (FKRP). Nestes casos, portanto, o exame molecular é de grande valor para a confirmação do diagnóstico. Objetivos: Analisar os genes CAPN3 e FKRP em pacientes com diagnóstico histológico de LGMD e verificar a expressão proteica da CAPN3 nesses pacientes, correlacionando com as mutações identificadas e com o quadro clínico e histológico dos mesmos. Resultados: Fizeram parte deste estudo 36 pacientes com LGMD provenientes do ambulatório de miopatias do HC-FMUSP em que a biópsia muscular não identificou deficiência de distrofina, disferlina, caveolina-3 e sarcoglicanas. Destes, nove (25%) foram diagnosticados com LGMD2A, seis (17%) com LGMD2I e em 21 (58%) não foi possível identificar o subtipo específico. Foram encontradas mutações patogênicas no gene CAPN3 em oito pacientes, sendo em homozigose em dois casos, heterozigose composta em cinco casos e em heterozigose em um caso. Em um caso o diagnóstico de LGMD2A foi realizado baseado apenas na análise da expressão da proteína CAPN3 no tecido muscular. Em seis pacientes foram identificadas mutações patogênicas no FKRP, sendo em homozigose em cinco casos e em heterozigose em um caso. A maioria dos pacientes com LGMD2I (cinco casos) apresentava a mutação c.826C > A. Foi observada ausência total ou parcial da expressão da CAPN3 em pacientes com LGMD2A. Conclusões: O presente estudo mostrou que mutações nos genes CAPN3 e FKRP são frequentes em pacientes com diagnóstico clínico e histológico de LGMD. A análise da expressão da CAPN3 se mostrou como uma importante ferramenta no diagnóstico da LGMD2A / Introduction: The Limb-Girdle Muscular Dystrophies (LGMD) are caused by mutations on a wide variety of genes that encode muscular proteins which can be inherited in dominant or recessive autosomal forms. The diagnosis is made either by genetic study or by muscle biopsy which shows a dystrophic histologic pattern with specific deficiency of muscular proteins. On some LGMD subtypes such as calpain-3 (CAPN3) and fukutin related protein (FKRP) deficiencies it is not possible to make a specific diagnosis by muscle biopsy. In these cases, the molecular exam is of great value to confirm the diagnosis. Objectives: Analyze the CAPN3 and FKRP genes in patients with histological diagnoses of LGMD, and verify the protein expression of CAPN3 on these patients correlating it with the identified mutations and their clinical and histological pattern. Results: Thirty-six patients with LGMD, where the muscular biopsy did not identify deficiency of dystrophin, dysferlin, caveolin-3 and sarcoglycans, from the Muscle Ambulatory of HC-FMUSP took part in this study. Of these, nine (25%) were diagnosed with LGMD2A, six (17%) with LGMD2I, and on 21 of them (58%), it was not possible to identify the specific subtype. Pathogenic mutations on CAPN3 were found in eight patients, being homozygous in two cases, compound heterozygous in five cases and heterozygous in one case. The diagnosis of LGMD2A in one patient was done based exclusively by CAPN3 protein analysis on the muscle tissue. Pathogenic mutations on FKRP were found in six patients, being homozygous in five cases and heterozygous in one case. Most of the patients with LGMD2I (five cases) presented the mutation c.826C > A. It was observed total or partial absence of the CAPN3 expression in patients with LGMD2A. Conclusions: The study showed that mutations on CAPN3 and FKRP are frequent in patients with clinical and histological diagnosis of LGMD. The CAPN3 expression analysis proved as an important tool in the LGMD2A diagnosis

Introdução: As distrofias de cinturas representam um grupo de miopatias progressivas, geneticamente determinadas, envolvendo 16 formas de herança autossômica recessiva e oito dominantes, sendo as formas recessivas mais comuns, particularmente em crianças. Caracterizam-se por fraqueza muscular progressiva de predomínio proximal em cinturas escapular e pélvica, existindo desde formas graves de início na infância a formas leves de início em adultos. A biópsia muscular, com estudo histológico e imunoistoquímico, é fundamental para o diagnóstico, porém o exame molecular é o teste padrão ouro para o diagnóstico de certeza. Objetivos: Determinar a freqüência dos diferentes subtipos de distrofia de cinturas em crianças na nossa população, descrevendo os aspectos clínicos, histológicos e moleculares. Resultados: Fizeram parte deste estudo 39 crianças provenientes do ambulatório de doenças neuromusculares do HC-FMUSP, sendo a proporção entre o sexo feminino e masculino de 3:1. A idade de início da doença variou de dois a 13 anos, com média de 7,5 anos. Os sinais e sintomas na apresentação clínica incluíram: quedas frequentes (22 casos), dificuldades em subir escadas (13 casos), marcha digitigrada (2 casos) e dificuldades para se levantar do chão (2 casos). Os níveis de CK foram elevados em todos os pacientes, sendo maiores naqueles com diferlinopatia e algumas formas de sarcoglicanopatias. Dentre os 39 pacientes, 37 foram classificados como LGMD. Destes, 15 (40,5%) receberam o diagnóstico de sarcoglicanopatia (LGMD2C-F), cinco (13,5%) de disferlinopatia (LGMD2B), cinco (13,5%) de calpainopatia, dois (5,5%) de LGMD1B, dois (5,5%) de LGMD2I, um (2,5%) de caveolinopatia (LGMD1A), e em sete (19%) não foi possível identificar o subtipo específico. A biópsia muscular mostrou um padrão distrófico em todos os casos, sendo mais acentuado nas sarcoglicanopatias e na LGMD2I. A presença de inflamação foi incomum na LGMD2B, e a presença de fibras lobuladas foi um achado marcante na LGMD2A. Conclusões: O diagnóstico do subtipo específico de LGMD em crianças é um desafio. Este estudo em crianças brasileiras provenientes de um centro de doenças neuromusculares de um grande hospital da rede pública mostrou alta frequência de sarcoglicanopatias, seguida por LGMD2A e LGMD2B. Já a LGMD2I parece ser incomum no Brasil / Background: Limb-girdle muscular dystrophies (LGMD) are a heterogeneous group of genetic muscular dystrophies, involving 16 autosomal recessive subtypes and eight autosomal dominant subtypes. Autosomal recessive dystrophy is far more common than autosomal dominant dystrophy, particularly in children. The clinical course in this group is characterized by progressive proximal weakness, initially in pelvic and after in shoulder-girdle musculature, varying from very mild to severe degree. Significant overlap of clinical phenotypes, with genetic and clinical heterogeneity, constitutes the rule for this group of diseases. Muscle biopsies are useful for histopathologic and immunolabeling studies, and DNA analysis is the gold standard to establish the specific form of muscular dystrophy. Objectives: The aim of this study was to characterize the clinical, histological and molecular aspects in children with LGMD who attend a big public neuromuscular centre in our country to determine the frequency of different forms. Results: Thirty seven patients were classified as LGMD and included in this analysis. The study period extended from 2009-2012. The female to male ratio was 3:1. The age of onset ranged from two to 13 years, mean 7,5 years. Onset in the first decade was seen in 90%. The initial clinical signs included: frequent falls (22 cases), difficulty in climbing stairs (13 cases), walk on tip toes (2 cases), difficulty in rising from the floor (2 cases) and difficulty on walking (1 case). The serum CK levels were high in all cases. Among the 37 patients, 15 (40,5%) were classified as sarcoglycanopathies (LGMD2C-F), five (13,5%) as dysferlinopathy (LGMD2B), five (13,5%) as calpainopathy (LGMD2A). Mutations in LMNA gene (LGMD1B), FKRP gene (LGMDI) and caveolin gene (LGMD 1C) were identified in two (5,5%), two (5,5%) and one patient (2,5%), respectively. In seven of 37 cases (19%) it was impossible to determine specific diagnosis. Calf hypertrophy, scapular winging and scoliosis were the most characteristic signs in sarcoglycanopathies. In LGMD2I calf hypertrophy is also observed. Atrophy of posterior compartment of thighs is frequent in children with LGMD2B and could suggest the diagnosis. In LGMD2A winging of scapulae and contractures in Achilles tendons were important findings. Muscle biopsy showed a dystrophic pattern in all cases, more intense in sarcoglycanopathies and LGMD2I. Differently from adult\'s patients, inflammation changes in dysferlinopaties were uncommon. Lobuled fibers were characteristic changes in calpainopathies in children. Conclusions: A definitive diagnosis among various subtypes of LGMD in children is challenging. Our series was a large study on LGMD in Brazilian children and showed high frequency of sarcoglycanopathies followed by LGMD2A, LGMD2B, LGMD2I, LGMD1B and LGMD1C

Introdução: As distrofias de cinturas representam um grupo de miopatias progressivas, geneticamente determinadas, envolvendo 16 formas de herança autossômica recessiva e oito dominantes, sendo as formas recessivas mais comuns, particularmente em crianças. Caracterizam-se por fraqueza muscular progressiva de predomínio proximal em cinturas escapular e pélvica, existindo desde formas graves de início na infância a formas leves de início em adultos. A biópsia muscular, com estudo histológico e imunoistoquímico, é fundamental para o diagnóstico, porém o exame molecular é o teste padrão ouro para o diagnóstico de certeza. Objetivos: Determinar a freqüência dos diferentes subtipos de distrofia de cinturas em crianças na nossa população, descrevendo os aspectos clínicos, histológicos e moleculares. Resultados: Fizeram parte deste estudo 39 crianças provenientes do ambulatório de doenças neuromusculares do HC-FMUSP, sendo a proporção entre o sexo feminino e masculino de 3:1. A idade de início da doença variou de dois a 13 anos, com média de 7,5 anos. Os sinais e sintomas na apresentação clínica incluíram: quedas frequentes (22 casos), dificuldades em subir escadas (13 casos), marcha digitigrada (2 casos) e dificuldades para se levantar do chão (2 casos). Os níveis de CK foram elevados em todos os pacientes, sendo maiores naqueles com diferlinopatia e algumas formas de sarcoglicanopatias. Dentre os 39 pacientes, 37 foram classificados como LGMD. Destes, 15 (40,5%) receberam o diagnóstico de sarcoglicanopatia (LGMD2C-F), cinco (13,5%) de disferlinopatia (LGMD2B), cinco (13,5%) de calpainopatia, dois (5,5%) de LGMD1B, dois (5,5%) de LGMD2I, um (2,5%) de caveolinopatia (LGMD1A), e em sete (19%) não foi possível identificar o subtipo específico. A biópsia muscular mostrou um padrão distrófico em todos os casos, sendo mais acentuado nas sarcoglicanopatias e na LGMD2I. A presença de inflamação foi incomum na LGMD2B, e a presença de fibras lobuladas foi um achado marcante na LGMD2A. Conclusões: O diagnóstico do subtipo específico de LGMD em crianças é um desafio. Este estudo em crianças brasileiras provenientes de um centro de doenças neuromusculares de um grande hospital da rede pública mostrou alta frequência de sarcoglicanopatias, seguida por LGMD2A e LGMD2B. Já a LGMD2I parece ser incomum no Brasil / Background: Limb-girdle muscular dystrophies (LGMD) are a heterogeneous group of genetic muscular dystrophies, involving 16 autosomal recessive subtypes and eight autosomal dominant subtypes. Autosomal recessive dystrophy is far more common than autosomal dominant dystrophy, particularly in children. The clinical course in this group is characterized by progressive proximal weakness, initially in pelvic and after in shoulder-girdle musculature, varying from very mild to severe degree. Significant overlap of clinical phenotypes, with genetic and clinical heterogeneity, constitutes the rule for this group of diseases. Muscle biopsies are useful for histopathologic and immunolabeling studies, and DNA analysis is the gold standard to establish the specific form of muscular dystrophy. Objectives: The aim of this study was to characterize the clinical, histological and molecular aspects in children with LGMD who attend a big public neuromuscular centre in our country to determine the frequency of different forms. Results: Thirty seven patients were classified as LGMD and included in this analysis. The study period extended from 2009-2012. The female to male ratio was 3:1. The age of onset ranged from two to 13 years, mean 7,5 years. Onset in the first decade was seen in 90%. The initial clinical signs included: frequent falls (22 cases), difficulty in climbing stairs (13 cases), walk on tip toes (2 cases), difficulty in rising from the floor (2 cases) and difficulty on walking (1 case). The serum CK levels were high in all cases. Among the 37 patients, 15 (40,5%) were classified as sarcoglycanopathies (LGMD2C-F), five (13,5%) as dysferlinopathy (LGMD2B), five (13,5%) as calpainopathy (LGMD2A). Mutations in LMNA gene (LGMD1B), FKRP gene (LGMDI) and caveolin gene (LGMD 1C) were identified in two (5,5%), two (5,5%) and one patient (2,5%), respectively. In seven of 37 cases (19%) it was impossible to determine specific diagnosis. Calf hypertrophy, scapular winging and scoliosis were the most characteristic signs in sarcoglycanopathies. In LGMD2I calf hypertrophy is also observed. Atrophy of posterior compartment of thighs is frequent in children with LGMD2B and could suggest the diagnosis. In LGMD2A winging of scapulae and contractures in Achilles tendons were important findings. Muscle biopsy showed a dystrophic pattern in all cases, more intense in sarcoglycanopathies and LGMD2I. Differently from adult\'s patients, inflammation changes in dysferlinopaties were uncommon. Lobuled fibers were characteristic changes in calpainopathies in children. Conclusions: A definitive diagnosis among various subtypes of LGMD in children is challenging. Our series was a large study on LGMD in Brazilian children and showed high frequency of sarcoglycanopathies followed by LGMD2A, LGMD2B, LGMD2I, LGMD1B and LGMD1C

Introdução: As distrofias musculares de cinturas (limb-girdle muscular dystrophies - LGMD) são causadas por mutações em uma grande variedade de genes que codificam proteínas musculares, podendo ser herdadas de forma autossômica dominante ou recessiva. O diagnóstico é feito tanto através de exame de biópsia muscular que mostra um padrão histológico distrófico ao lado de deficiência específica de proteínas musculares quanto por estudo genético. Em alguns subtipos de LGMD não é possível fazer o diagnóstico específico pela biópsia muscular, tais como na deficiência da calpaína-3 (CAPN3) e da proteína relacionada a fukutina (FKRP). Nestes casos, portanto, o exame molecular é de grande valor para a confirmação do diagnóstico. Objetivos: Analisar os genes CAPN3 e FKRP em pacientes com diagnóstico histológico de LGMD e verificar a expressão proteica da CAPN3 nesses pacientes, correlacionando com as mutações identificadas e com o quadro clínico e histológico dos mesmos. Resultados: Fizeram parte deste estudo 36 pacientes com LGMD provenientes do ambulatório de miopatias do HC-FMUSP em que a biópsia muscular não identificou deficiência de distrofina, disferlina, caveolina-3 e sarcoglicanas. Destes, nove (25%) foram diagnosticados com LGMD2A, seis (17%) com LGMD2I e em 21 (58%) não foi possível identificar o subtipo específico. Foram encontradas mutações patogênicas no gene CAPN3 em oito pacientes, sendo em homozigose em dois casos, heterozigose composta em cinco casos e em heterozigose em um caso. Em um caso o diagnóstico de LGMD2A foi realizado baseado apenas na análise da expressão da proteína CAPN3 no tecido muscular. Em seis pacientes foram identificadas mutações patogênicas no FKRP, sendo em homozigose em cinco casos e em heterozigose em um caso. A maioria dos pacientes com LGMD2I (cinco casos) apresentava a mutação c.826C > A. Foi observada ausência total ou parcial da expressão da CAPN3 em pacientes com LGMD2A. Conclusões: O presente estudo mostrou que mutações nos genes CAPN3 e FKRP são frequentes em pacientes com diagnóstico clínico e histológico de LGMD. A análise da expressão da CAPN3 se mostrou como uma importante ferramenta no diagnóstico da LGMD2A / Introduction: The Limb-Girdle Muscular Dystrophies (LGMD) are caused by mutations on a wide variety of genes that encode muscular proteins which can be inherited in dominant or recessive autosomal forms. The diagnosis is made either by genetic study or by muscle biopsy which shows a dystrophic histologic pattern with specific deficiency of muscular proteins. On some LGMD subtypes such as calpain-3 (CAPN3) and fukutin related protein (FKRP) deficiencies it is not possible to make a specific diagnosis by muscle biopsy. In these cases, the molecular exam is of great value to confirm the diagnosis. Objectives: Analyze the CAPN3 and FKRP genes in patients with histological diagnoses of LGMD, and verify the protein expression of CAPN3 on these patients correlating it with the identified mutations and their clinical and histological pattern. Results: Thirty-six patients with LGMD, where the muscular biopsy did not identify deficiency of dystrophin, dysferlin, caveolin-3 and sarcoglycans, from the Muscle Ambulatory of HC-FMUSP took part in this study. Of these, nine (25%) were diagnosed with LGMD2A, six (17%) with LGMD2I, and on 21 of them (58%), it was not possible to identify the specific subtype. Pathogenic mutations on CAPN3 were found in eight patients, being homozygous in two cases, compound heterozygous in five cases and heterozygous in one case. The diagnosis of LGMD2A in one patient was done based exclusively by CAPN3 protein analysis on the muscle tissue. Pathogenic mutations on FKRP were found in six patients, being homozygous in five cases and heterozygous in one case. Most of the patients with LGMD2I (five cases) presented the mutation c.826C > A. It was observed total or partial absence of the CAPN3 expression in patients with LGMD2A. Conclusions: The study showed that mutations on CAPN3 and FKRP are frequent in patients with clinical and histological diagnosis of LGMD. The CAPN3 expression analysis proved as an important tool in the LGMD2A diagnosis

Title Use of thermal imaging camera in evaluation of kinezio taping impact on shoulder girdle for female volleyball players Objectives Aim of this diploma thesis is to find out how kinezio taping affects the temperature of shoulder girdle during the physical load at female volleyball players' smash Methods The thesis is devided into theoretical part which serves as a basic for research in the second empirical part. The complex will consist of a total of 16 female volleyball players. During the measurement we will examine the effect of kinezio taping on the temperature change of shoulder girdle during the physical activity using the thermal camera before and after a physical activity. And at the remaining eight players we eill record the values before and after physical activity without apllied kinezio tape. Results Using the thermal imaging camera we found out that after application of kinezio tape the temperature in the shoulder girdle at rest raises gradually. The increase of temperature during physical load is in the reporting period lower with applied kinezio tape. Keywords Thermal camera, kinezio taping, shoulder girdle, temperature, blood flow. Powered by TCPDF (www.tcpdf.org)

The aim of this thesis was to evaluate the influence of Vojta's reflex locomotion on the activity of the shoulder girdle's muscles with surface electromyography. We also observed the activity of some of the trunk's muscles. We tested phasic and support function of the upper extremity in several movements on 15 healthy people. It didn't bring many significant values. It could be caused by different sensitivities of the participants for the stimulation, the short time of the stimulation, individual motoric stereotypes or not so strongly expressed muscle dysbalantion within healthy individuals.

Title: The effect of scapular specific kinesio tape on the movement quality of the upper limb. Objectives: The aim of this study is to evaluate the effectiveness of specific kinesio tape of shoulder blade on the quality of the movement of the upper limb. Currently, kinesio taping is very popular method in the treatment of myoskeletal diseases. Kinesio taping is mostly used by athletes, however the method becomes one of the common methods in physiotherapist practice. In this diploma thesis, specific method of kinesio taping was used. The method is focused on the facilitation of the lower blade fixators and is frequently used. Positive effect of the method on the position of the shoulder joint is obvious. The movement quality of the upper limb is represented by visual motor skills coordination, which was objectified with selected testing method. The prerequisite is a close relation between the position of the shoulder girdle with the function of the hand. Centred position of the shoulder blade ensured with the fixation of the lower blade fixators allows precise movement of the entire upper limb. The diploma thesis was focused on the population of people working on a computer. This way of working is now the most common cause of pain for patients who visit a physiotherapist. Measurement was attended by...

As células-tronco podem ser isoladas tanto de tecidos embrionários quanto de tecidos provenientes de um organismo adulto. Este projeto teve por objetivo caracterizar, descrever as células derivadas da região uterina e da cinta placentária junção materno/fetal da placenta de carnívoros domésticos (cães e gatos), e verificar a sua capacidade de pluripotência. Os úteros gestantes e não gestantes foram obtidos em campanhas de castrações e de controle populacional de cães e gatos, na cidade de Pirassununga/SP. Foram coletados 24 úteros gravídicos de animais hígidos, em diferentes idades gestacionais. O material foi dividido em três fases distintas da gestação, ou seja inicio que compreende de 8 a 20 dias de gestação; meio de 21 a 30 dias de gestação e final de 31 a 60 dias de gestação. O material foi coletado de fêmeas caninas e felinas, quatro úteros de cada fase, totalizando 12 úteros de cães e 12 de felinos. Coletamos também 8 úteros de fêmeas nulíparas (4 de cadelas e 4 de gatas) e 8 úteros com um mês pós parto (4 de cadelas e 4 de gatas). As amostras foram fixadas em paraformoldeido tamponado a 4% para a análise histológica e de imunohistoquimica. Para a padronização da imunohistoquimica inúmeros testes de marcação e diluição dos anticorpos utilizados nesta pesquisa foram realizados, todo protocolo aqui descrito foi padronizado pela primeira vez. Nas análises de imunohistoquimica avaliamos a expressão de marcadores associados a células-tronco pluripotentes Nanog, Oct4 e Sox2. Nas cadelas, as marcações foram positivas em todas as fases, gestacionais e não gestacionais. A detecção dessas proteínas nesta espécie ficaram padronizadas, destacando algumas diferenças quantitativas durante alguns períodos da gestação. Foi observado que o Oct4 na cadela, mostra uma diferença significativa (p=0,0064), entre as fases de início e meio da gestação e entra o início e a fase de termo. Quando comparados os resultados das análises imunohistoquimicas utilizando os três anticorpos entre si, nos três períodos gestacionais ficou evidente uma diferença (p=0,0005) somente relativa a proteína Nanog com Oct4. Nas gatas apenas foi possível padronizar o protocolo do Nanog e do Sox2, sendo a marcação feita com Oct4 negativa. Nesta espécie foi possível observar uma diferença da proteína Nanog (p=0,0006) quando comparada na fase inicial para a fase do meio e início da gestação para a fase termo. No que se refere as fêmeas nulíparas e fêmeas pós-parto destaca-se a ausência de diferenças quando comparados os anticorpos na fase pós parto tanto em cadelas quanto em gatas. Na fase nulípara foram observadas diferenças somente na cadela (p=0,0018) para os três anticorpos. Desta forma, a caracterização de células de origem placentária com característica de células tronco pode abrir um leque de possibilidades para obtenção destas células de forma mais ética, uma vez que este material é descartado na castrações. Foi possível a identificação das células que expressão proteínas pluripotentes em diferentes idades gestacionais, tanto na região de cinta placentária como no útero. Apesar de semelhantes, as espécies aqui estudadas apresentaram diferenças na realização do protocolo da imunihistoquímica. Pesquisas relacionadas com as células-tronco do endométrio vêm crescendo, principalmente porque estas células podem ser facilmente obtidas, a partir de fontes descartadas, sem entraves éticos. Desta forma tem o potencial de serem uma nova fonte para o desenvolvimento na terapêutica como terapia celular / The stem cells can be isolated both embryonic tissues as much tissues from an adult organism. This project had from objective characterize and describe cells derived from uterine region and placental girdle - maternal junction / fetal placenta of domestics carnivores (dogs and cats), and check their capacity of pluripotency. The uterus pregnant and non-pregnants were obtained in castrations campaigns and population control of dogs and cats in the city of Pirassununga/SP. Were collected 24 uterus gravidic of healthy animals at different gestational ages. The material was split into three separate stages of pregnancy,that is, the Startconsist of 8 to 20 days of gestation;between21-30 days of gestation and end 31-60 days of gestation. The material was colleted from canine and feline females, four uterus each phase 12 uterus of dogs and 12 uterus of cats. We also collect 8 nulliparous female uterus (4 of dogs and 4 of cats) and 8 uterus with a postpartum month (4 of dogs and 4 of cats). The samples were fixed in paraformoldeido at 4% for histological analysis and immunohistochemical. For the standardization of immunohistochemical, were made numerous tests of marking and dilution of antibodies all the protocol described here has been standardized for the first time. In immunohistochemical analysis, we evaluated the expression of markers associated with pluripotent stem cells Nanog, Oct4 and Sox2. In cats, it was only possible to standardize the protocol of the Nanog and of the Sox2, and marking made with negative Oct4. This species was possible was possible to observe a difference in the Nanog protein (p = 0.0006) compared with the initial phase to the medium and early pregnancy to the term phase. As regards the nulliparous females and postpartum females there is the absence of differences when compared to the antibodies in postpartum stage, both in dogs and in cats. In nulliparous phase differences were only observed in dogs (p = 0.0018) for the three antibodies. Thus, the characterization of placental origin cells with stem cell characteristic can open up a range of possibilities for obtaining such cells more ethical, since this material is discarded in castration. The identification of pluripotent cells expressing proteins at different gestational ages was possible in both placental belt region as in utero. Although similar, the species studied here showed differences in the realization of immunohistochemical protocol. Searches related to the endometrial stem cells are growing, mainly because these cells can be easily obtained from sources discarded without ethical barriers. This way has the potential to be a new source for development in the therapeutic cell therapy