Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 6 uppsatser.

Thesis (Ph. D.)--Cornell University, May, 2007. / Vita. Includes bibliographical references (leaves 134-143).

Orientadores: Maria Elvira Pizzigatti Corrêa, Cecília Amélia Fazzio Escanhoela / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-20T05:39:33Z (GMT). No. of bitstreams: 1 Soares_TaniaCristinaBenetti_D.pdf: 3640740 bytes, checksum: 3cc935c816cada9809557953ff7cae3e (MD5) Previous issue date: 2012 / Resumo: A doença do enxerto contra o hospedeiro crônica (DECHc) é um processo inflamatório aloimune o qual resulta da resposta celular (células T) do doador contra o receptor em pacientes tratados pelo transplante de células progenitoras hematopoiéticas (TCTH). Os órgãos mais afetados são pele, mucosa oral, glândulas salivares e fígado. O objetivo deste trabalho foi avaliar os achados histopatológicos das glândulas salivares menores (GSM) e fígado dos pacientes afetados pela DECHc, comparando-os entre si, com as amostras destes órgãos de pacientes que não desenvolveram DECHc pós TCTH e com pacientes que não foram tratados pelo TCTH. Amostras de GSM e fígado de pacientes tratados por TCTH mieloablativo, de doadores aparentados com HLA idêntico, entre 1994 e 2006, foram analisadas. Cinquenta e sete pacientes foram selecionados, sendo 36 com DECHc oral e hepática e 21 sem DECH. O diagnóstico de DECHc foi definido por critérios clínicos (extensa/localizada), laboratoriais e de seguimento. Amostras sem alterações de pacientes não transplantados também foram avaliadas (19 de GSM e 20 de fígado). Os espécimes foram corados em hematoxilina e eosina, ácido periódico de Schiff (PAS), Tricrômio de Masson, Reticulina e Perls e tratados pela técnica de imuno-histoquímica para CD45, CD45RO, CD68, CD4, CD8, CD138 e AE1/AE3. Os critérios definidos pela classificação proposta pelo grupo de trabalho em histopatologia da reunião de consenso do National Institutes of Health (NIH) foram empregados para a avaliação de todas as amostras de ambos os órgãos. Nos espécimes corados pelo PAS, foi tomada a medida da área acinar das GSM, em imagens digitalizadas. No fígado de pacientes que desenvolveram DECH, foi observado aumento estatisticamente significante do número de células imunomarcadas para CD8 e CD45RO, quando comparado ao dos outros 2 grupos...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital / Abstract: Chronic graft-versus-host disease (cGVHD) is an alloimmune inflammatory process, which results from a donor-origin cellular response (T cells) against host tissues. The organs mostly affected are skin, oral mucosa, salivary glands and liver. The aim of this study was to evaluate the histopathological findings of minor salivary glands (MSG) and liver of patients affected by cGVHD, comparing the findings each other and with those of the patients who did not develop cGVHD after HSCT and patients who were not underwent HSCT. MSG and liver samples from patients who underwent myeloablative HLA-matched HSCT from sibling donors, between 1994 and 2006, were analyzed. Fifty-seven patients were selected, being 36 with oral and hepatic cGVHD and 21 without GVHD. The diagnosis of cGVHD was defined through clinical/ laboratory criteria and follow-up. Samples from non-transplanted patients were also evaluated (19 MSG and 20 liver specimens). The specimens were stained with haematoxylin & eosin, periodic acid-Schiff (PAS), Masson's trichrome, reticulin and Perls, and immunolabeled for CD45, CD45RO, CD68, CD4, CD8, CD138, and AE1/AE3. The criteria defined in the classification proposed by the consensus meeting of the National Institutes of Health were used for the evaluation of all samples of both organs. Digital images of the PAS stained sections were used to estimate the MSG acinar area. In the liver of patients who developed GVHD it was observed a statistically significant increase in the number of CD45RO and CD8 immunostained cells, comparatively with the other 2 groups...Note: The complete abstract is available with the full electronic document / Doutorado / Patologia / Doutor em Estomatopatologia

Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.

Current immune suppressive strategies fail to induce donor-recipient immune tolerance after allogeneic hematopoietic cell transplantation. Accordingly, patients suffer morbidity and mortality from graft vs. host disease (GVHD) and prolonged immune suppressive therapy. Biologic insight into transplantation tolerance is needed, and translation of such insight to novel clinical strategies may improve clinical outcomes. We report original investigation at seminal phases of this process including initial prophylactic immune suppression, onset of acute graft vs. host disease, and ultimate immune suppression discontinuation: In a controlled randomized clinical trial, we demonstrate that sirolimus-based immune suppression reduces risk for acute GVHD, ameliorates the severity of subsequent chronic GVHD, and supports reconstitution of functional regulatory T cells. Study of tissue-infiltrating CD4+ T cell subsets in acute GVHD target organs supports a pathogenic role for Th17 cells. Finally, we demonstrate that peripheral blood transcriptional biomarkers provide mechanistic insight into human transplantation tolerance. These data signal progress, and suggest rational translational efforts to achieve transplantation tolerance.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2005. / Härtill 5 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 5 uppsatser.

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 5 uppsatser.

Diss. (sammanfattning) Umeå : Univ., 2007. / Härtill 4 uppsatser.