Due to difficulties in elucidating neurobiological aspects of psychological disorders, the National Institute of Mental Health (NIMH) created the Research Domain Criteria (RDoC), which encourages novel conceptualizations of the relationship between neurobiological circuitry and clinical difficulties. This approach is markedly different from the Diagnostic and Statistical Manual of Mental Disorders (DSM) based approach that has dominated clinical research to date. Thus, RDoC necessitates exploration of novel experimental and statistical approaches. Fear learning paradigms represent a promising methodology for elucidating connections between acute threat (“fear”) circuitry and fear-related clinical difficulties. However, traditional analytical approaches rely on central tendency statistics, which are tethered to a priori categories and assume homogeneity within groups. Growth Mixture Modeling (GMM) methods such as Latent Class Growth Analysis (LCGA) may be uniquely suited for examining fear learning phenotypes. However, just three extant studies have applied GMM to fear learning and only one did so in a human population. Thus, the degree to which classes identified in known studies represent characteristics of the general population and to which GMM methodology is applicable across populations and paradigms is unclear. This preliminary study applied LCGA to a fear learning lab study in an attempt to identify heterogeneity in fear learning patterns based on a posteriori classification. The findings of this investigation may inform efforts to move toward a trans-diagnostic conceptualization of fear learning. Consistent with the goals laid out in RDoC, explication of fear learning phenotypes may eventually provide critical information needed to spur innovation in psychotherapeutic and psychopharmacological treatment. / Master of Science / To date, most clinical psychology research has been based on the Diagnostic and Statistical Manual of Mental Disorders (DSM), which is a catalog of mental health disorders that was originally designed to facilitate communication among clinicians. Many experts contend that this approach has hampered progress in the field of biological clinical psychology research. Thus, the National Institute of Mental Health (NIMH) created a new template for biological clinical psychology research called the Research Domain Criteria (RDoC). Since RDoC calls for a complete overhaul in the conceptualization of clinical dysfunction, this approach requires statistical and experimental innovation. One traditional experimental approach that may be helpful in understanding the RDoC topic of acute threat (“fear”) is called Pavlovian Fear Learning (PFL). However, traditional PFL studies have utilized statistical methods that are based on comparing group averages and require researchers to determine groups of interest based on theory before the study begins. This is problematic because RDoC calls for research that begins with evidence rather than theory. Growth Mixture Modeling (GMM) is a statistical methodology that may allow researchers to analyze fear learning data without having to begin with theoretically determined categories such as DSM disorders. However, little research has tested how well this approach would work. This study is just the second to apply a GMM approach to a human PFL study. The findings from this investigation may inform efforts to develop a statistical technique that is well suited for RDoCian research and may also spur innovation in psychotherapeutic and psychopharmacological treatment.

Norovirus som orsakar vinterkräksjukan är en av de vanligaste vintersjukdomarna i Sverige. Sjukdomstiden varar generellt i en till tre dagar med symptomen kräkning och/eller diarré. Till den totala sjukdomsbilden världen över gällande akut gastroenterit, bidrar norovirus med 18 %. Trots att sjukdomen är mycket vanlig är kunskapen om norovirusets förfarande till stor del okänd.Syftet med studien var att göra en tidsserieanalys, även så kallad One-Step Growth analys, av koncentrationen minus-RNA i celler som infekterats med olika koncentrationer av murint norovirus (MNV). För att detektera minus-RNA användes RT-qPCR med SYBR Green. Målet var att se om startkoncentrationerna av virus vid någon tidpunkt korrelerar med mängden minus-RNA i cellerna. Efter 4 och 8 timmar fanns ett exponentiellt samband mellan den initiala viruskoncentrationen och minus-RNA-uttrycket i cellerna. Koncentrationen minus-RNA i de infekterade cellerna ökade mellan de undersökta tiderna 4, 8 och 24 timmar. Vidare visade resultaten att det krävs 4 timmar för att minus-RNA skulle vara kvantifierbar vid en högre infektionskoncentration av viruspartiklar, medan det krävs 24 timmar för den lägre infektionskoncentrationen av viruspartiklar. / Norovirus causes winter vomiting disease and is one of the commonest cause of winter illness in Sweden. The disease period generally lasts one to three days with symptoms like vomiting and/or diarrhea. To the disease burden of acute gastroenteritis worldwide, norovirus contributes with 18 %. Even though the illness is very common, the knowledge about norovirus is poor and largely unknown.The purpose of the study was to do a time series analysis, a so-called One-Step Growth analysis, of the minus-RNA concentration in cells infected with different concentrations of murine norovirus (MNV). For the detection of minus-RNA RT-qPCR was used with SYBR Green. The goal was to correlate start concentration of virus at any time with the amount of minus-RNA in the cells. At 4 and 8 hours there was an exponential connection by the initial virus concentration and minus-RNA development in the cells. The concentration of minus-RNA in the infected cells increased between 4, 8 and 24 hours. Further, the results can be interpreted as requiring 4 hours for the higher concentrations to become quantifiable, while requiring 24 hours for the lower concentrations to become quantifiable.

Made available in DSpace on 2016-04-28T14:58:32Z (GMT). No. of bitstreams: 1 2007- Jose Antonio Monte.pdf: 871031 bytes, checksum: 637d77aaf96168f58214d17d66e559fd (MD5) Previous issue date: 2002-02-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / An experiment was conducted to evaluate the influence of the irrigation schedule on fruit production and quality, biomass production in the different parts of the plant, and the physiological growth parameters of the tomato plants, cultivar D?bora for salad, cultivated in the field, in a split plot design, in the Federal Rural University of Rio de Janeiro, from 21/09/2005 to 31/01/2006. The experiment was conducted with four treatments and four replications of different irrigation schedules, using a drip irrigation system with sprinklers spaced 0,3m with a water volume of 1,14 l.h-1. During the essay, six samplings of the plants were collected, for evalua tion of the plant development, through the growth analysis of the different parts of the plant. Mathematical equations were adjusted for the mass accumulation in root, stems, leaves and fruits, and for leaf area of plants. The analysis of the results showed that the irrigation schedule of three days caused lowers values of the physiological parameters, a tendency of reduction of big fruits production and higher number of small fruits. In these experimental conditions, the irrigation schedule of two days is the best recommendation for the farmer because the quality of the tomato production is the same of those under more frequent irrigation but with a smaller water use. / Com o objetivo de avaliar a influ?ncia do turno de rega na produ??o e qualidade de frutos, na acumula??o de fitomassa nas diferentes partes da planta, e na obten??o dos ?ndices fisiol?gicos de crescimento da cultura de tomateiro para mesa da cultivar D?bora, tipo longa vida, realizou-se um experimento em faixas, na Universidade Federal Rural do Rio de Janeiro, no per?odo de 21/09/2005 ? 31/01/2006. O experimento foi feito com quatro tratamentos consistindo de diferentes freq??ncias de irriga??o, atrav?s de um sistema de irriga??o localizada com emissores espa?ados de 0,3 m e de vaz?o correspondente a 1,14 l.h-1, tendo quatro repeti??es. Durante o experimento foram realizadas seis coletas de plantas para avaliar o desenvolvimento vegetal, atrav?s da an?lise do crescimento, em seis diferentes ?pocas ap?s o transplante. Foram ajustadas equa??es matem?ticas para o ac?mulo de massa, nas ra?zes, nos caules, nas folhas e nos frutos, e para a ?rea foliar das plantas. Analisando os resultados observou-se que o turno de rega com intervalo de tr?s dias entre irriga??es apresentou menores ?ndices fisiol?gicos, menor tend?ncia em produzir frutos grandes e apresentou maiores n?meros de frutos mi?dos. Nas condi??es experimentais, o turno de rega alternado de 2 dias foi o mais recomendado para o agricultor, por ter a mesma qualidade de produ??o de tomate apresentada pelos turnos de regas mais freq?entes, mas com menr gasto de ?gua.

Made available in DSpace on 2016-04-28T14:58:59Z (GMT). No. of bitstreams: 1 2006-Rubens Nei Briacon Busquet.pdf: 998513 bytes, checksum: 2e2a50b0c0141ff91d47dae53e3ba216 (MD5) Previous issue date: 2006-03-28 / This work had the objective of to analyze the development pattern and the nutrient accumulation of four genotypes of banana, with micropropagated plantlets, in the conditions of the State of Rio de Janeiro. An experiment was driven in the Department of Fitotecnia of UFRRJ, in Planossolo, in a randomized blocks design with four treatments and four repetitions of 45 plants for plot, in the spacing of 3x2 m. Four genotypes of the banana were evaluated: Thap Maeo (AAB), FHIA-18 (AAAB), SH3640 (AAAB) and Prata An? (AAB). Only the mother plant was driven per hole, being eliminated the whole sucker. In the first stage, leaves of the four genotypes were sampled eight weeks after transplant (WAT), to determine the correction factor to estimate the leaf area from the length and width of foliar limbo. The angular coefficients of the regression revealed that there are significant differences among genotypes, indicating that in the determination of the leaf area of banana tree by ruler, the factor of correction of 0.79 should be used for cultivar SH 3640, FHIA 18 and Thap Maeo, and 0.83 for Prata An?. The second stage, the temperature and the precipitation arise as the climate factors more related to the rate of leaf emission of the four genotypes.. The genotype Thap Maeo presented larger vegetative cycle (366 days) and total (468 days) and larger plant height (3.0 m) that the other genotypes. The genotypes didn't present differences in the reproductive cycle and in the diameter of plants. The genotype Fhia 18 presented the largest total mass of fruits for plant. The genotype Thap Maeo presented larger medium number of bunches for bunch, the largest of number of fruits for bunch, the largest relationship pulp-peel, however the smallest fruit size. The genotype SH 3640, although presented fruits of greatest weight and size, presented the smallest number of fruits for bunch and the pulp-peel ratio. In the third stage, to quantify the time variation of plant biomass and nutrient accumulation, one plant was sampled per plot on 8 harvests spaced 8 weeks, when the leaf area measured, and the mass and concentrations of N, P, K, Ca, Mg measured in each plant portion. The growth rates and of nutrient accumulation were measured through the functional plant growth analysis. The mass of rhizomes and stems increased up to 48 WAT and stabilized at 56 WAT. The leaf area, and the mass of active leaves and petioles increased up to 40 WAT and stabilizing in the last three harvests. The net assimilation rate increased between 8 and 24 WAT, and declined mainly after 32 SAT. The rates of nutrient accumulation presented the same pattern regarding with the time of maximal accumulation, varying from 30 to 33 WAT. From the total of nutrients accumulated by the banana plant, the bunch exported after fruit harvest 50% of N, 38% of P, 60% of K, 11% of Ca and 14% of Mg. The total amount of nutrient accumulated, in kg per ha, was 54.5 of N, 14.4 of P, 131.5 of K, 62,5 of Ca and 53 of Mg. . / Este trabalho teve como objetivo analisar o padr?o de crescimento e acumula??o de nutrientes e o comportamento fenol?gico de quatro gen?tipos de bananeiras (Musa spp.), com mudas produzidas por micro-propaga??o, nas condi??es do Estado do Rio de Janeiro. Foi conduzido um experimento no Departamento de Fitotecnia da UFRRJ, em Planossolo, em delineamento em blocos ao acaso com quatro tratamentos e quatro repeti??es de 45 plantas por parcela, no espa?amento de 3x2 m. Foram avaliados quatro cultivares de banana: Thap Maeo (AAB), FHIA-18 (AAAB), SH3640 (AAAB) e Prata An? (AAB). Foi conduzida apenas a planta m?e por touceira, eliminando-se a brota??o lateral. Na primeira etapa foi feita a coleta de folhas, com o objetivo de determinar o fator de corre??o para estimar a ?rea foliar a partir do comprimento e largura de limbo foliar. Houve diferen?as significativas entre gen?tipos, indicando que deve-se usar o fator de corre??o de 0,79 para a as cultivares SH3640, FHIA 18 e Thap Maeo, e 0,83 para Prata An?. A segunda etapa teve como objetivo verificar a taxa de lan?amento de folhas de quatro gen?tipos de bananeiras e correlacion?-la com as condi??es clim?ticas e estudar o comportamento fenol?gico dos quatro gen?tipos. A temperatura e a precipita??o apresentaram-se como os fatores de clima mais relacionados ? taxa de emiss?o de folhas pelos quatro gen?tipos. Os gen?tipos apresentaram comportamento diferenciado quanto ? taxa de lan?amento de folhas nos per?odos 8, 32 e 64 SAT, quando o gen?tipo Prata An? apresentou o maior n?mero de folhas lan?adas. O gen?tipo Thap Maeo apresentou maior ciclo vegetativo (366 dias) e total (468 dias) e maior altura de planta que os demais gen?tipos. O gen?tipo Fhia 18 apresentou a maior massa total de frutos por planta. O gen?tipo Thap Maeo apresentou maior n?mero m?dio de pencas e frutos por cacho, a maior rela??o polpa-casca, por?m o menor tamanho de fruto. O gen?tipo Prata An? apresentou o valor mais elevado de s?lidos sol?veis totais. O gen?tipo SH 3640, apresentou o pior desempenho. Na terceira etapa, para quantificar a varia??o temporal de biomassa vegetal e de acumula??o de nutrientes, foram efetuadas 8 coletas espa?adas 8 semanas, quando foi coletada uma planta por parcela, separada em rizoma, limbo foliar, bainha das folhas, pec?olo, enga?o, flores, br?cteas e frutos, e determinando-se a ?rea foliar. No material vegetal obtido em cada coleta foram determinados os teores de N, P, K, Ca, Mg. As taxas de crescimento e de acumula??o de nutrientes foram mensuradas atrav?s do m?todo funcional de an?lise de crescimento vegetal. A massa seca do rizoma e do pseudocaule aumentou significativamente at? 48 SAT, se estabilizando aos 56 SAT. A ?rea foliar, e a massa das folhas ativas e dos pec?olos aumentaram at? 40 SAT, se estabilizando nas tr?s ?ltimas coletas.A ?rea foliar espec?fica reduziu-se ao longo das coletas em todos os gen?tipos, sendo que Fhia 18 apresentou os menores valores e Thap Maeo os maiores valores de ?rea foliar espec?fica. A taxa de assimila??o l?quida aumentou entre 8 SAT e 24 SAT, quando decaiu principalmente a partir de 32 SAT. As taxas de acumula??o absoluta de N, P, K, Ca e Mg apresentaram-se similares quanto a ?poca de m?xima acumula??o, variando de 30 a 33 SAT.. Ap?s a colheita, do total de nutrientes imobilizados pela bananeira, foram exportados para o cacho 50% do N, 38% do P, 60% do K, 11% do Ca e 14% do Mg. O total de nutrientes acumulado, em kg por ha, foi 54,5 de N, 14,4 de P, 131,5 de K, 62,5 de Ca e 53 de Mg.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-23T14:27:46Z No. of bitstreams: 1 2011 - Jos? Ant?nio Monte.pdf: 1144390 bytes, checksum: 831746c40c4d98b1c97c23c831d74f7c (MD5) / Made available in DSpace on 2016-08-23T14:27:46Z (GMT). No. of bitstreams: 1 2011 - Jos? Ant?nio Monte.pdf: 1144390 bytes, checksum: 831746c40c4d98b1c97c23c831d74f7c (MD5) Previous issue date: 2011-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The irrigation regime for ?in natura? tomato cultivation was studied at the experimental area of the Horticulture sector of the Crop Science Department of the Universidade Federal Rural do Rio de Janeiro, located at Serop?dica-RJ. The first experiment was realized to evaluate the effect of the water lamina (40, 60, 80, 100 e 120% of ETc) on biomass and foliar area accumulation, to obtain growth parameters for a growth analysis and production of the culture, for Debora plus hybrid, a long life type. In the second essay, the influence of the irrigation schedules (TR0 every day; TR1 every two days; TR2 every three days; and TR3 every four days) was investigated, by the growth analysis and production of the same hybrid. In the third experiment, the growth and production was studied for three genotypes (Debora plus hybrid, a long life type; Carmem hybrid; and the variety Santa Clara), all for in natura use, all under the same irrigation regime. The results obtained in the first experiment demonstrated that the maximal biomass accumulation occurs between 70 and 80 days after transplanting, for all treatments. The quantity of water applied above 80% of ETc resulted in higher vegetative growth and general production, but with the same commercial production as for the others treatments. Above 80% of th Etc, there was an augmentation of defective fruits, with a higher input for the culture, using more water and energy, without an augmentation of commercial fruits of tomato. In the second essay, there was no difference among the irrigation schedules and, therefore, the longer schedule of every four days had the same fruit quality as the others schedules. The third experiment showed differences for the growth pattern and water and nutrient requirements of the genotypes. The quantity of water used by the Carmem hybrid was lower than for Debora hybrid, which indicated the necessity of detailed studies with different irrigation strategies for each genotype of tomato. Therefore, for an optimization of inputs use for the ?regulated deficit irrigation?, the irrigation management needs to be adequate to the different requirement, during the cycle, of each genotype / Estudou-se o manejo de irriga??o na cultura do tomateiro para mesa, na ?rea experimental pertencente ao Setor de Horticultura, do Departamento de Fitotecnia na Universidade Federal Rural do Rio de Janeiro, localizada no Munic?pio de Serop?dica-RJ, em tr?s experimentos de campo com o objetivo de foi estudar o desenvolvimento da cultura do tomateiro em rela??o ? capacidade de produ??o de fitomassa e a de frutos, em diferentes formas de aplica??o de ?gua atrav?s da irriga??o. Buscando maior efici?ncia no uso da de ?gua de irriga??o, com produ??o de tomate de qualidade vi?vel economicamente para o produtor e sustent?vel para o meio ambiente. O primeiro experimento foi realizado para avaliar a influ?ncia da l?mina de irriga??o 40, 60, 80, 100 e 120% da evapotranspira??o (ETc) da cultura na do tomateiro na acumula??o de fitomassa e ?rea foliar, e obten??o de ?ndices fisiol?gicos, para uma an?lise de crescimento da cultura e produ??o de frutos, do tomateiro h?brido D?bora plus, tipo longa vida. O segundo experimento foi conduzido para avaliar a influ?ncia do turno de rega (TR1 turno de rega di?rio; TR2 turno de rega de dois dias; TR3 turno de rega de tr?s dias; e TR4 turno de rega de quaro dias) no crescimento e na produ??o de frutos, do mesmo tomateiro. E, no terceiro experimento, avaliou-se tamb?m o crescimento e a produ??o de frutos de tr?s gen?tipos (os h?bridos, longa vida, D?bora Plus e o Carmem e a variedade Santa Clara), todos para mesa, sob o mesmo regime de irriga??o. Com base nos resultados obtidos, no primeiro experimento, foi poss?vel constatar que o m?ximo de ac?mulo de fitomassa ocorreu entre 70 e 80 dias ap?s o transplante, em todos os tratamentos. O aumento na quantidade de ?gua aplicada acima de 80 % da ETc resultou em maior crescimento vegetativo do tomateiro e incremento na produ??o total de frutos, por?m, com a mesma produ??o comercial de frutos das l?minas menores. Nas irriga??es acima de 80% da ETc, houve um aumento na produ??o de frutos defeituosos de tomate, tornando a cultura mais onerosa, com maior gasto de energia e ?gua, sem aumentar a produ??o comercial de frutos do tomateiro. No segundo experimento, observou-se que n?o houve diferen?a entre os tratamentos, e por isso o turno de rega mais longo, de tr?s dias, produz a mesma qualidade de frutos de tomate dos turnos de regas mais freq?entes, mas com maior potencial de economia de ?gua e energia. O terceiro experimento mostrou que existem grandes diferen?as quanto ao padr?o de crescimento e ?s exig?ncias, tanto h?drica quanto nutricional, dos diferentes gen?tipos de tomateiros. A quantidade de ?gua exigida pelo h?brido Carmem ? inferior a quantidade de ?gua exigida pelo h?brido D?bora, indicando a necessidade de estudos mais detalhados com estrat?gias de irriga??o nos diferentes gen?tipos de tomateiro. Portanto, para haver uma otimiza??o dos recursos aplicados o manejo da irriga??o, usando a ?irriga??o sob d?ficit controlado?, essa dever? atender as diferentes exig?ncias, ao longo do ciclo, de cada gen?tipo.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-09T19:14:10Z No. of bitstreams: 1 2009 - Evandro Silva Pereira Costa.pdf: 2009437 bytes, checksum: cf64a3e8f7e3e004841a52c65f4c6626 (MD5) / Made available in DSpace on 2017-02-09T19:14:10Z (GMT). No. of bitstreams: 1 2009 - Evandro Silva Pereira Costa.pdf: 2009437 bytes, checksum: cf64a3e8f7e3e004841a52c65f4c6626 (MD5) Previous issue date: 2009-09-21 / CNPq / Defining methods of quantification of tomato late blight, caused by Phytophthora infestans, is important to many researches about strategies of management of the disease. The aim of this present study was to assess and to validate the diagrammatic grading keys - Simplified, James-modified and Broad - and to determinate the possible correlations between the measures of severity and plants? physiological and nutritional state and biomass accumulation. Two simultaneous experiments were carried out: one in greenhouse and the other in field conditions. Two cultivars were studied in greenhouses, Super Sweet and ?Perinha ?gua Branca?, a resistant and a susceptible to late blight genotypes, respectively. The plants were inoculated or not with a sporangia suspension, and submitted to ten evaluations: 1, 2, 3, 4, 6, 7, 9, 10, 17 and 24 days after inoculation. The avaliations were performed in the fifth oldest leaf, and eighth and eleventh leaves, by visual estimation of severity, using three diagrammatic grading keys, Simplified, Broad and James-modified, but also measuring photosynthetic potential (Fv/Fm), by chlorophyll ?a? fluorescence emission. In field conditions, four cultivars were evaluated ? ?Perinha ?gua Branca?, Super Sweet, Santa Clara and Carmen F1. Periodically, late blight severity was quantified using the same three grading keys in leaves of different plant parts, at 51, 62, 74, 94 and 102 days after transplant (DAT). At the day following each severity disease evaluation, one plant was harvested per plot to measure the accumulation of biomass and macronutrients of different plant organs. Fruit yield was determined by summing fruits harvested between 68 and 114 DAT. In greenhouse conditions, the estimative of late blight severity with the three diagrammatic grading keys proved to be adequate to the disease?s quantification, enabling the discrimination between inoculation effects and differences between the cultivars in four and in nine days after inoculation, respectively, which were confirmed by measures of the photosynthetic potential of the leaves. In field conditions, the diagrammatic grading keys Simplified and Broad were more adequate to tomato late blight quantification. Quantifications in the youngest leaves, in the medium and superior third part of the plant, were the most representative of the disease progress and also discriminated the cultivars regarding disease resistance. Negative correlations were observed between severity, estimated with the three grading keys considering the whole plant and its medium third part, and leaf dry mass and leaf concentrations of K and P. The cultivar ?Perinha ?gua Branca? showed the lowest stem and leaf dry mass at the beginning of plant evaluations, and also late fruit yield, but had the higher stem and leaf mass and at the end of the experiment. The cultivar Perinha ?gua Branca and the hybrid Carmen F1 had higher commercial fruit yield. Based in the results, it is recommended the use of Simplified diagrammatic grading key and evaluations in the medium or superior tomato third to quantify the late blight disease. / A defini??o de m?todos de quantifica??o da requeima no tomateiro, causada por Phytophthora infestans, ? importante para v?rias pesquisas sobre estrat?gias de manejo da doen?a. Esse trabalho teve como objetivo aferir e validar as escalas diagram?ticas, Simplificada, James-modificada e Detalhada e determinar as poss?veis correla??es entre as medidas de severidade e o estado fisiol?gico e nutricional da planta e o ac?mulo de biomassa. Realizaram-se dois experimentos simult?neos, um sob condi??es de ambiente protegido e o outro sob condi??es de campo. Em casa de vegeta??o, utilizaram-se duas cultivares de tomate cereja, ?Perinha ?gua Branca? e Super Sweet, suscet?vel e resistente ? requeima, respectivamente. As plantas foram inoculadas ou n?o com suspens?o de espor?ngios de P. infestans e em seguida, avaliadas aos 1, 2, 3, 4, 6, 7, 9, 10, 17 e 24 dias ap?s a inocula??o. As avalia??es foram feitas na quinta, oitava e d?cima primeira folha, contadas do ?pice para a base, usando as tr?s escalas acima citadas e medi??es do potencial fotossint?tico (Fv/Fm) pela emiss?o da clorofila ?a?. No campo, utilizaram-se quatro cultivares, ?Perinha ?gua Branca?, Super Sweet, Santa Clara e Carmen F1. Periodicamente, quantificou-se a severidade da requeima utilizando-se as mesmas escalas acima citadas em folhas de diferentes partes da planta, aos 51, 62, 74, 94 e 102 dias ap?s o transplante (DAT). No dia seguinte a cada avalia??o de severidade, foi coletada uma planta por parcela para mensurar o ac?mulo de biomassa e de macronutrientes nos diferentes ?rg?os da planta. A produ??o de frutos foi determinada pelo somat?rio das colheitas realizadas entre 68 e 114 DAT. Em condi??es de casa de vegeta??o, a estimativa da severidade da requeima com as tr?s escalas se mostrou adequada para quantifica??o da doen?a tendo permitido discriminar efeito de inocula??o e diferen?as entre as cultivares aos quatro e nove dias ap?s a inocula??o, respectivamente, que foram confirmadas pelas medidas do potencial fotossint?tico das folhas. Em condi??es de campo, as escalas Simplificada e Detalhada, foram mais adequadas para a quantifica??o da requeima do tomateiro. Quantifica??es feitas nas folhas mais jovens, no ter?o m?dio e superior da planta, foram as que melhor representaram o progresso da doen?a e discriminaram as cultivares quanto a resist?ncia ? doen?a. Observaram-se correla??es negativas entre a severidade, estimada com as tr?s escalas, considerando a planta inteira e o ter?o m?dio, e a massa seca de folhas e os teores foliares de K e P nas quatro cultivares. A cultivar ?Perinha ?gua Branca? apresentou menor massa seca de caule e de folha no in?cio das avalia??es e produ??o de frutos mais tardia, por?m ao final do ciclo destacou-se pela maior massa seca de caule e de folha. A cultivar ?Perinha ?gua Branca? e o h?brido Carmen F1 apresentaram maior produ??o de frutos comerciais. Com base nos resultados pode-se recomendar o uso da escala simplificada e avalia??es no te?o m?dio ou superior do tomateiro para quantifica??o da requeima.

Made available in DSpace on 2017-07-10T19:25:00Z (GMT). No. of bitstreams: 1 Adriana Maria Meneghetti.pdf: 723411 bytes, checksum: fc151b405deaed517334646d5726cad4 (MD5) Previous issue date: 2005-11-28 / This research aimed on evaluating the growth and production of corn (Zea mays L.) in order to obtain the baby corn, which was submitted to the variation of four different depth irrigations, recorded from the culture s evapotranspiration, based on the class A, and applied when the evapotranspiration reached these following accumulated values: T1-15 mm, T2- 30 mm, T3- 45 mm e T4- 60 mm. The statistical model was outlinement completely with four treatments and five replications. Results were submitted to the statistical analysis, by the Sisvar program (FERREIRA 2000). The analysis of variance and the averages from the treatments were also recorded, as well as compared considering 5 % of probability according to the Tukey test and regression analysis. The experimental plots had about 8 m of length; the plants were separated by 0.080m among themselves and 0.70 m by rows, totalizing 100 plots to each plot. The features regarding the growth during the culture s cycle were: plant height (PE), foliar area (FA), leaf number (LN), foliar area index (FAI). Some other features were analyzed regarding the production analysis as: number of ear by plant (NEP), height of insertion of the first ear (HIFE), length of the ear with and without straw (LEWOS), diameter of the ear with and without straw (DEWOS), mass of the ear with and without straw (MEWOS), number of the row of grains by ear (NRGE) and number of ear, accomplishing 360 g. There were significant differences on production results regarding data of growing of foliar area and foliar area index. Significant differences had been also observed in the production of HIFE and LEWOS, and DEWOS, MEWOS and MEWOS, NRGE and number of ear that accomplishes 360 g. There was an increase in the depth irrigation and also in the application frequency, which implied in greater values of foliar area, as well as in the foliar area index, number of ear by plant, height of insertion of the first ear, number of row of grains by ear and number of ear, accomplishing 360 g, but also lower values for height, leaf number and length, diameter and ear s mass, with and without straw. The Best commercial results were recorded with T1 15 mm, when using 18 % of ear. The scheduling irrigation for baby corn must be carried out with application when the crop evapotranspiration indicates 15 mm and 30 mm accumulated values. It could also be understood with this trial that greater values cause reduction on productivity. / O objetivo deste trabalho foi avaliar o crescimento e produção de milho (Zea mays L.) para obtenção de minimilho. As parcelas em análise foram submetidas) a quatro lâminas de irrigação determinadas a partir da evapotranspiração da cultura, baseadas no tanque classe A e aplicadas quando a evapotranspiração atingia os valores acumulados: T1-15 mm, T2-30 mm, T3-45 mm e T4-60 mm. O modelo estatístico utilizado foi em parcelas subdivididas com quatro tratamentos e cinco repetições. Os resultados foram submetidos à análise estatística, pelo programa Sisvar (FERREIRA, 2000). Determinou-se a análise de variância e as médias dos tratamentos foram comparadas em nível de 5 % de probabilidade pelo teste de Tukey e pela análise de regressão. As parcelas experimentais possuíam comprimento de 8 m, compostas por plantas separadas entre si por 0,080 m e 0,70 m entre linhas, com um total de 100 plantas por parcela. As características para a análise de crescimento foram: estatura da planta (EP), número de folhas (NF), área foliar (AF), índice de área foliar (IAF). Para a análise de produção foram analisadas: número de espigas por planta (NE), estatura de inserção da primeira espiga (EI), comprimento da espiga com palha (CECP), comprimento da espiga sem palha (CESP), diâmetro da espiga com palha (DECP), diâmetro da espiga sem palha (DESP), massa da espiga com palha (MECP), massa da espiga sem palha (MESP), número de fileira de grãos por espiga (NF E-1) e número de espigas que perfazem 360 g. Houve diferenças significativas para os dados de crescimento nos valores de área foliar e índice de área foliar. Também foram observadas diferenças significativas nos resultados de produção para estatura de inserção da primeira espiga, comprimento da espiga com e sem palha, diâmetro da espiga com e sem palha, massa da espiga com e sem palha, número de fileiras de grãos por espiga e número de espigas que perfazem 360 g. Um aumento nas lâminas de irrigação e na freqüência de aplicação implicou em maiores valores de área foliar e índice de área foliar, número de espigas, estatura de inserção da espiga, número de fileira de grãos por espiga e o número de espigas em 360 g, e menores valores para estatura, número de folhas e comprimento, diâmetro e massa da espiga com e sem palha. Os melhores resultados comerciais obtidos foram com o T1-15 mm, cujo aproveitamento foi de 18 % das espiguetas. O manejo da irrigação do minimilho deve ser realizado com aplicação quando a evapotranspiração da cultura indicar valores acumulados de 15 mm a 30 mm. Valores maiores ocasionam redução na produtividade.

Made available in DSpace on 2017-07-10T19:25:10Z (GMT). No. of bitstreams: 1 Nayara.pdf: 589475 bytes, checksum: f2c35283a861de2856bf3240ffe1381c (MD5) Previous issue date: 2012-06-20 / The seeds produced by Crambe abyssinica Hochst (crambe) species have great potential in the biodiesel industry that currently searches for new sources of biomass to reach its production goals. As this culture species is rarely developed, it is essential to carry out researches on ecophysiological behavior to implement it in the Brazilian energy matrix. In order to characterize the ecophysiological behavior of crambe, this study evaluated the growth and allelopathic potential of crambe under conditions of water stress in two stages. vi Crambe plants were submitted to different levels (4, 8, 12, 16 and 20 days) of irrigation cancelation during the vegetative and flowering stages. At each level, the following parameters were analyzed: number of leaves, plants heights, leaf area and total dry weight and calculated physiological indexes of leaf area ratio (LAR), specific leaf area (SLA), net assimilation rate (NAR) and relative growth rate (RGR), all analyzed by linear exponential regression. In addition, it was carried out a leaf constitution analysis of micro and macronutrients by atomic absorption spectrophotometry. The static aqueous extract was prepared from its shoots to evaluate the allelopathic potential of crambe under water stress, at 2 and 4% concentration and then added to Petri dishes with 25 seeds of lettuce for each one. Experiments were carried out in a germination chamber with distilled water and osmotic potential (PEG 6000) as control treatments, based on a completely randomized design. The obtained data were percentage (%G) and germination speed index (GSI), root (RL) and shoot lengths (SL) of seedlings and averages were compared by Tukey test at 5% significance level. Crambe plants showed some decrease on leaf area and total dry matter in the vegetative stage, in the last level of stress. For the same developmental stage, an effect of water stress was observed at LAR, SLA, NAR and RGR indexes, concluding that this stage of development is more sensitive to drought effects. The extracts that were prepared at 4% concentration showed higher allelopathic effects. During vegetative development, there was a decrease in allelopathic potential of plants along the stress, but, during flowering, crambe plants affected negatively germination and seedling initial development of lettuce during the 4th and 16th days of water stress. Thus, it was concluded that the cancelation of irrigation on crambe plants differed in terms of development period and intensity of water stress / As sementes produzidas pela espécie Crambe abyssinica Hoscht (crambe) apresentam grande potencial na indústria do biodiesel que, atualmente, procura novas fontes de biomassa vegetal para atingir as metas de produção. Por ser uma espécie de cultivo pouco desenvolvida, pesquisas sobre seu comportamento ecofisiológico são importantes para auxiliar a implementar tal cultura na matriz energética brasileira. A fim de caracterizar o comportamento ecofisiológico do crambe, o presente estudo avaliou o crescimento e a potencialidade alelopática do crambe em condições de estresse hídrico em dois estádios de desenvolvimento. Plantas de crambe foram submetidas a diferentes níveis (4, 8, 12, 16 e 20 dias) de suspensão de irrigação durante o estádio vegetativo e a floração. Em cada nível, foram analisados os seguintes parâmetros: número de folhas, altura das plantas, área foliar e massa seca total e calculados os índices fisiológicos de razão de área foliar (RAF), área foliar específica (AFE), taxa de assimilação liquida (TAL) e taxa de crescimento relativa (TCR), submetidos à análise de regressão linear exponencial. Além disso, fez-se análise da constituição foliar de micro e macronutrientes por espectrofotometria de absorção atômica. Para avaliar a potencialidade alelopática do crambe em condições de estresse hídrico, foi preparado extrato aquoso estático a partir de suas partes aéreas, em concentração de 2 e 4%, os quais foram adicionados em placas de Petri contendo 25 sementes de alface em cada uma. Os experimentos foram conduzidos em câmara de germinação, com testemunha de água destilada e de potencial osmótico (PEG 6000) e delineamento inteiramente casualizado. Obtiveram-se porcentagem (%G), índice de velocidade de germinação (IVG), comprimento médio de raiz (CMR) e de parte aérea (CMPA) das plântulas e as médias comparadas pelo teste Tukey a 5% de significância. As plantas de crambe apresentaram redução de área foliar e massa seca total no estádio vegetativo, no último nível de estresse. Para o mesmo estádio de desenvolvimento, foi observado efeito do estresse hídrico nos índices RAF, AFE, TAL e TCR, haja vista esse estádio de desenvolvimento ser mais sensível aos efeitos do déficit hídrico. Os extratos preparados a 4% de concentração apresentaram maiores efeitos alelopáticos. Durante o desenvolvimento vegetativo, observou-se redução na potencialidade alelopática das plantas ao longo do estresse. Porém, na floração, as plantas afetaram negativamente o processo de germinação e desenvolvimento inicial das plântulas de alface durante o 4° e 16° dia de estresse hídrico. Assim, conclui-se que os efeitos da suspensão de irrigação sobre as plantas de crambe variaram quanto ao período de desenvolvimento e à intensidade do estresse hídrico

Made available in DSpace on 2017-05-12T14:48:21Z (GMT). No. of bitstreams: 1 Adriana Maria Meneghetti.pdf: 723411 bytes, checksum: fc151b405deaed517334646d5726cad4 (MD5) Previous issue date: 2005-11-28 / This research aimed on evaluating the growth and production of corn (Zea mays L.) in order to obtain the baby corn, which was submitted to the variation of four different depth irrigations, recorded from the culture s evapotranspiration, based on the class A, and applied when the evapotranspiration reached these following accumulated values: T1-15 mm, T2- 30 mm, T3- 45 mm e T4- 60 mm. The statistical model was outlinement completely with four treatments and five replications. Results were submitted to the statistical analysis, by the Sisvar program (FERREIRA 2000). The analysis of variance and the averages from the treatments were also recorded, as well as compared considering 5 % of probability according to the Tukey test and regression analysis. The experimental plots had about 8 m of length; the plants were separated by 0.080m among themselves and 0.70 m by rows, totalizing 100 plots to each plot. The features regarding the growth during the culture s cycle were: plant height (PE), foliar area (FA), leaf number (LN), foliar area index (FAI). Some other features were analyzed regarding the production analysis as: number of ear by plant (NEP), height of insertion of the first ear (HIFE), length of the ear with and without straw (LEWOS), diameter of the ear with and without straw (DEWOS), mass of the ear with and without straw (MEWOS), number of the row of grains by ear (NRGE) and number of ear, accomplishing 360 g. There were significant differences on production results regarding data of growing of foliar area and foliar area index. Significant differences had been also observed in the production of HIFE and LEWOS, and DEWOS, MEWOS and MEWOS, NRGE and number of ear that accomplishes 360 g. There was an increase in the depth irrigation and also in the application frequency, which implied in greater values of foliar area, as well as in the foliar area index, number of ear by plant, height of insertion of the first ear, number of row of grains by ear and number of ear, accomplishing 360 g, but also lower values for height, leaf number and length, diameter and ear s mass, with and without straw. The Best commercial results were recorded with T1 15 mm, when using 18 % of ear. The scheduling irrigation for baby corn must be carried out with application when the crop evapotranspiration indicates 15 mm and 30 mm accumulated values. It could also be understood with this trial that greater values cause reduction on productivity. / O objetivo deste trabalho foi avaliar o crescimento e produção de milho (Zea mays L.) para obtenção de minimilho. As parcelas em análise foram submetidas) a quatro lâminas de irrigação determinadas a partir da evapotranspiração da cultura, baseadas no tanque classe A e aplicadas quando a evapotranspiração atingia os valores acumulados: T1-15 mm, T2-30 mm, T3-45 mm e T4-60 mm. O modelo estatístico utilizado foi em parcelas subdivididas com quatro tratamentos e cinco repetições. Os resultados foram submetidos à análise estatística, pelo programa Sisvar (FERREIRA, 2000). Determinou-se a análise de variância e as médias dos tratamentos foram comparadas em nível de 5 % de probabilidade pelo teste de Tukey e pela análise de regressão. As parcelas experimentais possuíam comprimento de 8 m, compostas por plantas separadas entre si por 0,080 m e 0,70 m entre linhas, com um total de 100 plantas por parcela. As características para a análise de crescimento foram: estatura da planta (EP), número de folhas (NF), área foliar (AF), índice de área foliar (IAF). Para a análise de produção foram analisadas: número de espigas por planta (NE), estatura de inserção da primeira espiga (EI), comprimento da espiga com palha (CECP), comprimento da espiga sem palha (CESP), diâmetro da espiga com palha (DECP), diâmetro da espiga sem palha (DESP), massa da espiga com palha (MECP), massa da espiga sem palha (MESP), número de fileira de grãos por espiga (NF E-1) e número de espigas que perfazem 360 g. Houve diferenças significativas para os dados de crescimento nos valores de área foliar e índice de área foliar. Também foram observadas diferenças significativas nos resultados de produção para estatura de inserção da primeira espiga, comprimento da espiga com e sem palha, diâmetro da espiga com e sem palha, massa da espiga com e sem palha, número de fileiras de grãos por espiga e número de espigas que perfazem 360 g. Um aumento nas lâminas de irrigação e na freqüência de aplicação implicou em maiores valores de área foliar e índice de área foliar, número de espigas, estatura de inserção da espiga, número de fileira de grãos por espiga e o número de espigas em 360 g, e menores valores para estatura, número de folhas e comprimento, diâmetro e massa da espiga com e sem palha. Os melhores resultados comerciais obtidos foram com o T1-15 mm, cujo aproveitamento foi de 18 % das espiguetas. O manejo da irrigação do minimilho deve ser realizado com aplicação quando a evapotranspiração da cultura indicar valores acumulados de 15 mm a 30 mm. Valores maiores ocasionam redução na produtividade.

Made available in DSpace on 2017-05-12T14:48:34Z (GMT). No. of bitstreams: 1 Nayara.pdf: 589475 bytes, checksum: f2c35283a861de2856bf3240ffe1381c (MD5) Previous issue date: 2012-06-20 / The seeds produced by Crambe abyssinica Hochst (crambe) species have great potential in the biodiesel industry that currently searches for new sources of biomass to reach its production goals. As this culture species is rarely developed, it is essential to carry out researches on ecophysiological behavior to implement it in the Brazilian energy matrix. In order to characterize the ecophysiological behavior of crambe, this study evaluated the growth and allelopathic potential of crambe under conditions of water stress in two stages. vi Crambe plants were submitted to different levels (4, 8, 12, 16 and 20 days) of irrigation cancelation during the vegetative and flowering stages. At each level, the following parameters were analyzed: number of leaves, plants heights, leaf area and total dry weight and calculated physiological indexes of leaf area ratio (LAR), specific leaf area (SLA), net assimilation rate (NAR) and relative growth rate (RGR), all analyzed by linear exponential regression. In addition, it was carried out a leaf constitution analysis of micro and macronutrients by atomic absorption spectrophotometry. The static aqueous extract was prepared from its shoots to evaluate the allelopathic potential of crambe under water stress, at 2 and 4% concentration and then added to Petri dishes with 25 seeds of lettuce for each one. Experiments were carried out in a germination chamber with distilled water and osmotic potential (PEG 6000) as control treatments, based on a completely randomized design. The obtained data were percentage (%G) and germination speed index (GSI), root (RL) and shoot lengths (SL) of seedlings and averages were compared by Tukey test at 5% significance level. Crambe plants showed some decrease on leaf area and total dry matter in the vegetative stage, in the last level of stress. For the same developmental stage, an effect of water stress was observed at LAR, SLA, NAR and RGR indexes, concluding that this stage of development is more sensitive to drought effects. The extracts that were prepared at 4% concentration showed higher allelopathic effects. During vegetative development, there was a decrease in allelopathic potential of plants along the stress, but, during flowering, crambe plants affected negatively germination and seedling initial development of lettuce during the 4th and 16th days of water stress. Thus, it was concluded that the cancelation of irrigation on crambe plants differed in terms of development period and intensity of water stress / As sementes produzidas pela espécie Crambe abyssinica Hoscht (crambe) apresentam grande potencial na indústria do biodiesel que, atualmente, procura novas fontes de biomassa vegetal para atingir as metas de produção. Por ser uma espécie de cultivo pouco desenvolvida, pesquisas sobre seu comportamento ecofisiológico são importantes para auxiliar a implementar tal cultura na matriz energética brasileira. A fim de caracterizar o comportamento ecofisiológico do crambe, o presente estudo avaliou o crescimento e a potencialidade alelopática do crambe em condições de estresse hídrico em dois estádios de desenvolvimento. Plantas de crambe foram submetidas a diferentes níveis (4, 8, 12, 16 e 20 dias) de suspensão de irrigação durante o estádio vegetativo e a floração. Em cada nível, foram analisados os seguintes parâmetros: número de folhas, altura das plantas, área foliar e massa seca total e calculados os índices fisiológicos de razão de área foliar (RAF), área foliar específica (AFE), taxa de assimilação liquida (TAL) e taxa de crescimento relativa (TCR), submetidos à análise de regressão linear exponencial. Além disso, fez-se análise da constituição foliar de micro e macronutrientes por espectrofotometria de absorção atômica. Para avaliar a potencialidade alelopática do crambe em condições de estresse hídrico, foi preparado extrato aquoso estático a partir de suas partes aéreas, em concentração de 2 e 4%, os quais foram adicionados em placas de Petri contendo 25 sementes de alface em cada uma. Os experimentos foram conduzidos em câmara de germinação, com testemunha de água destilada e de potencial osmótico (PEG 6000) e delineamento inteiramente casualizado. Obtiveram-se porcentagem (%G), índice de velocidade de germinação (IVG), comprimento médio de raiz (CMR) e de parte aérea (CMPA) das plântulas e as médias comparadas pelo teste Tukey a 5% de significância. As plantas de crambe apresentaram redução de área foliar e massa seca total no estádio vegetativo, no último nível de estresse. Para o mesmo estádio de desenvolvimento, foi observado efeito do estresse hídrico nos índices RAF, AFE, TAL e TCR, haja vista esse estádio de desenvolvimento ser mais sensível aos efeitos do déficit hídrico. Os extratos preparados a 4% de concentração apresentaram maiores efeitos alelopáticos. Durante o desenvolvimento vegetativo, observou-se redução na potencialidade alelopática das plantas ao longo do estresse. Porém, na floração, as plantas afetaram negativamente o processo de germinação e desenvolvimento inicial das plântulas de alface durante o 4° e 16° dia de estresse hídrico. Assim, conclui-se que os efeitos da suspensão de irrigação sobre as plantas de crambe variaram quanto ao período de desenvolvimento e à intensidade do estresse hídrico