O objetivo deste trabalho foi avaliar os efeitos da suplementação parenteral com vitamina E e selênio em determinados parâmetros hemáticos e séricos usados comumente na clínica diária em cavalos de enduro. Foram utilizados seis cavalos em treinamento das raças Árabes e Anglo-árabes em um delineamento em Cross Over de dois períodos de 84 dias cada, divididos em grupos Tratamento e Controle. Não foi observado efeito de tratamento para as variáveis peso, hematócrito, contagem eritrocitária, hemoglobina, leucócitos, freqüência cardíaca, concentração de hemoglobina corpuscular média, uréia, glutationa peroxidase, creatinina lactatodesidrogenase, creatiniquinase, gammaglutamiltransferase e glutationa peroxidase. Os valores de enzimas lactatodesidrogenase e creatiniquinase em repouso ficaram muito por cima dos níveis de referência em ambos grupos. No dia 70 foi observada diferença significativa (p < 0,05) para volume corpuscular médio e hemoglobina corpuscular média, correspondendo o valor menor ao grupo tratamento. As conclusões extraídas deste estudo são: 1. A suplementação parenteral de selênio e vitamina E não influi nos parâmetros analisados em cavalos que treinam e competem em provas de até 60 km. 2. Apesar de inconstantes, os achados nos valores de volume corpuscular médio e hemoglobina corpuscular média podem sinalizar uma tendência de adaptação ao exercício destes valores hematimétricos em animais com melhor defesa antioxidante. / The aim of this study was to measure the effects of parenteral supplementation with selenium and vitamin E in predetermined hematic and serics parameters commonly used in endurance horse daily clinic. Six Arab and Anglo-Arab horses in endurance training were used in a Cross Over experimental design of two periods of 84 days each, divided in Treatment and Control groups. No treatment effects was detected for weight, packed cell volume, red blood cell count, hemoglobin, leucocytes, heart rate, mean corpuscular hemoglobin concentration, reduced glutathion, urea, creatinine and lactatodeshidrogenase, creatinikinase, gammaglutamiltransferase or glutathion peroxidase. The lactatedeshidrogenase and creatinikinase values were high than those used as reference in both groups. In 70\'th day was observed a statistical difference (p < 0,05) for mean corpuscular volume and mean corpuscular hemoglobin parameters, with the lowest value in the treatment group. The following conclusions were extracted from this trial: 1. A parenteral supplementation with selenium and vitamin E had no effect in the examined parameters in horses training and competing in races till 60 km. 2. Despite the inconsistence of mean corpuscular volume and mean corpuscular hemoglobin finds, this observation can indicate an exercise adaptation tendency of this hematimetric values in horses with better antioxidant defense.

Orientador: Afonso Celso Vigorito / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-20T03:28:20Z (GMT). No. of bitstreams: 1 Souza_ClarissaVasconcellosde_M.pdf: 2809498 bytes, checksum: ac6c5e1a4f9ab38a684ec0a6a1a664a6 (MD5) Previous issue date: 2011 / Resumo: O procedimento de transplante de células-tronco hematopoiéticas (TCTH) pode causar perdas funcionais que dificultam atividades diárias que requeiram habilidades físicas. Nosso objetivo foi avaliar a função dos pacientes pré e após o TCTH utilizando uma avaliação em ambiente ambulatorial. De novembro de 2008 a Novembro de 2010, 50 pacientes, 29 (58%) mulheres, mediana de idade de 48 anos (24-67), foram arrolados no estudo. A coleta foi realizada pré e pós TCTH alogênico ou autólogo. Os instrumentos utilizados foram o teste de caminhada de 2 minutos (TC2M), saturação de oxigênio (SatO2), freqüência cardíaca (FC) e escala modificada de Borg (EMB) antes e depois do TC2M para avaliação da performance da marcha, teste de força de preensão (TFP) para avaliação de força muscular, teste de Schober (TS) para avaliação de mobilidade de coluna lombar e escala máxima e adaptada de atividade (EMA e EAA) do questionário Perfil de Atividade Humana (PAH) para avaliação da função física. Cinqüenta pacientes foram avaliados pré TCTH; 6 não foram submetidos ao TCTH; 3 morreram previamente, 1 se recusou e 2 foram excluídos. Quarenta e quatro de 50 (88%) foram submetidos ao TCTH, 21 alogênicos e 23 autólogos. Trinta e três de 44 (75%) pacientes foram submetidos à ambas as avaliações, 11/44 (25%) pacientes não foram submetidos: 9 morreram e 2 foram excluídos. Entre os grupos que foram submetidos às duas avaliações, achamos valores estatisticamente menores na avaliação pós para TC2M (p= 0,004), TFP para mão direita e esquerda (p= 0,004 e < 0,0001), TS, EMA e EAA (p< 0,0001); e valores maiores para FC (p= 0,01) antes do TC2M e SatO2 (p= 0,02) após o TC2M. Também encontramos diferenças estatisticamente significantes entre os grupos de pacientes autólogo e alogênico nas variáveis Hemoglobina (Hb), EMA (p= 0,002) e EAA (p= 0,008) no pós TCTH. Estas diferenças indicam um decréscimo no condicionamento aeróbico antes do stress físico, redução da performance da marcha, da força muscular, da flexibilidade da coluna e na função em atividades de vida diária no pós TCTH, mostrando perdas físicas nesta população. Ademais, o prejuízo funcional e físico foi mais severo nos pacientes submetidos ao procedimento alogênico no pós TCTH. Concluindo, o entendimento da intensidade e a especificidade dessas perdas físicas podem ajudar a conduzir melhor um programa de reabilitação no pós TCTH / Abstract: Hematopoietic stem cell transplant (HSCT) procedure may cause functional losses that impair daily tasks that require physical skills. Our aim was to evaluate function of patients pre and post HSCT using an assessment in outpatient basis. From November 2008 to November 2010, 50 patients, 29 (58%) female, median age 48 years (24-67), were enrolled in the study. Collection was performed pre and post autologous or allogeneic HSCT. Instruments were 2 minutes walking test (2MWT), oxygen saturation (Sa02), heart rate (HR) and Borg Scale (BS) before and after 2MWT for gate performance evaluation; Grip Strength (GS) for strength evaluation, Schober Test (ST) for spine mobility testing and maximum and adapted activity score (MAS and AAS) of Human Activity Profile (HAP) questionnaire for function role evaluation. Fifty patients were evaluated pre HSCT; 6 did not undergo HSCT; 3 died, 1 refused, and two were excluded. Forty four out of 50 (88%) underwent HSCT, 21 allogeneic, 23 autologous. Thirty three out of 44 (75%) patients performed both evaluations, 11/44 (25%) patients did not: 9 died and 2 were excluded. Among groups who performed both evaluations, we found significant lower values in the post evaluation for 2MWT (p= 0.004), GS for right and left hand (p= 0.004 and <0.0001 respectively), ST, MAS and AAS (p< 0.0001); and higher HR (p= 0.01) before 2MWT and Sa02 (p=0.02) after 2MWT. We also found statistically differences between allogeneic and autologous groups of patients for hemoglobin (Hb), MAS (p= 0.002) and AAS (p= 0.008) variables on post HSCT. Those differences indicate decrease on aerobic conditioning before physical stress, decline of gate performance, hand strength, spine flexibility and on function role on daily activities post HSCT, showing physical losses in this population. Moreover, functional and physical prejudice was more severe in patients that underwent allogeneic procedure on post HSCT. In conclusion a better understanding of intensity and specificity of those physical losses may help to conduct a better rehabilitation program on post HSCT period / Mestrado / Ciencias Basicas / Mestre em Clinica Medica

The objectives of this study were to characterize the hematological and productive pattern during the peripartum in cows with and without anemia, as well as to evaluate the erythrogram in animals that presented metritis puerperal acute (MPA), lameness, acetonemia, increased non-esterified fatty acids (NEFA) and cows with different categorization of body condition score (BCS). In study 1, 50 Holstein cows (29 multiparous and 21 primiparous) were used. Blood samples and physical examination were performed at 13 different times: 18 &#177; 3, 12 &#177; 2, 5 &#177; 1, and 2 &#177; 1 before calving, and 7, 14, 21, 30, 45 and 60 days postpartum. Erythrogram evaluations were performed, and red blood cell count (RBC), hemoglobin concentration, packed cell volum (PCV), and absolute hematimetric values of mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin, RDW, serum concentrations of iron, betahydroxybutyrate (BHBA), AGNE and total bilirubin (TBIL) were determined for the biochemical analyzes. Anemia was classified according to hemoglobin values &lt;7 g / dL and globular volume &lt;24%. The prevalence of anemia was higher in the period 60 days postpartum affecting 18.3% of the animals, the pattern of anemia presented was normocytic, normochromic, regenerative. RBC, PCV and hemoglobin were lower (P &lt;0.05) for animals with anemia. While the animals with MPA and lameness the value of the erythrogram was very similar throughout the postpartum period. No difference was observed between the groups according to the values of NEFA, BCS and loss of BCS during the periods performed in the postpartum period. In conclusion the anemic animals presented the values of the physical examination according to the physiological limits, the anemia did not cause productive losses in the affected animals. It was not evidenced that animals with MPA and claudicants presented a greater reduction of blood crass, excluding the occurrence of inflammatory anemia, and although the prevalence of anemia increased during postpartum, it was not possible to characterize the main cause of the reduction of hematological values between the categorizations. In the second study, blood was sampled from 336 animals (252 multiparous and 84 primiparous) between 21 and 30 days in lactation (DEL) on 7 farms in the State of São Paulo with the objective of characterizing the prevalence of anemia in dairy cows, prevalence of anemia in different categorizations such as: lactation number, BCS at the time of collection, dystocia, retention of fetal membranes, mastitis, digestive problem, lameness and acetonemia, , as well as the analysis erythrogram, biochemical profile, BCS, and milk production among animals with and without anemia. In conclusion, the prevalence of anemia was low (16.3%) and no association with the categorizations performed in this study, it was not possible to characterize the cause of anemia through biochemical analysis. / Os objetivos deste estudo foi caracterizar o padrão hematológico e produtivo durante o periparto em vacas com e sem anemia, assim como avaliar o eritrograma em animais que apresentaram metrite puerperal aguda (MPA), claudicação, acetonemia, valores de ácidos graxos não esterificados aumentados (AGNE) e vacas com diferentes categorizações de escore de condição corporal (ECC). No estudo 1, foram utilizadas 50 vacas Holandesas (29 multíparas e 21 primíparas), foram realizadas colheitas sanguíneas e exame físicos em 13 momentos diferentes: 18 &#177; 3, 12 &#177; 2, 5 &#177; 1, e 2 &#177; 1 antes do parto, parto, e 7, 14, 21, 30, 45 e 60 dias após o parto. Foram realizadas avaliações do eritrograma sendo mensurado a contagem de hemácias (RBC), concentração de hemoglobina, volume globular, além dos valores hematimétricos absolutos do volume corpuscular médio (VCM), concentração hemoglobínica corpuscular média (CHCM), hemoglobina corpuscular média (HCM) e RDW, para as análises bioquímicas foram determinadas a concentração sérica de ferro, betahidróxidobutirato (BHBA), AGNE e bilirrubina total (TBIL). Anemia foi classificada de acordo com os valores de hemoglobina &lt; 7 g/dL e o volume globular &lt; 24 %. A prevalência de anemia foi maior no período 60 dias após o parto afetando 18.3% dos animais, o padrão da anemia apresentado foi normocítica, normocrômica, regenerativa. Os valores das contagens de hemácias, volume globular e hemoglobina foram menores (P &lt; 0.05) para os animais com anemia. Enquanto os animais com MPA e com claudicação, o valor do eritrograma foi muito semelhante ao longo do pós-parto. Não foi observado diferença entre os grupos de acordo com os valores de AGNE, ECC e perda de ECC durante as coletas realizadas no pós-parto. Em conclusão os animais anêmicos apresentaram os valores do exame físico de acordo com os limites fisiológicos, a anemia não provocou perdas produtivas nos animais afetados. Não foi evidenciado que animais com MPA e claudicantes apresentassem maior redução da crase sanguínea, excluindo a ocorrência de anemia inflamatória, sendo que embora a prevalência de anemia aumentasse durante o pós-parto não foi possível caracterizar a causa principal da redução dos valores hematológicos entre as categorizações realizadas. No estudo 2, foram realizadas coletas sanguíneas em 336 animais (252 multíparas e 84 primíparas) entre 21 e 30 dias em lactação (DEL) em 7 fazendas no Estado de São Paulo com objetivo de caracterizar a prevalência de anemia nos animais, prevalência de anemia em diferentes categorizações realizadas como: distocia, retenção dos anexos fetais, mastite, problema digestivo, claudicação, acetonemia, ECC no momento da coleta e número de parto, assim como a análise do eritrograma, bioquímico e ECC e produção de leite entre os animais com e sem anemia. Em conclusão a prevalência de anemia foi baixa prevalência (16.3 %) e nenhuma associação com as categorizações realizadas nesse estudo, adicionalmente não foi possível caracterizar por meio de análises bioquímicas a causa da anemia.

No description available.

How temperature affects physiological development in the chicken embryo is unknown. Embryos incubated at 38°C or 35°C showed no difference in growth or survival. The time to hatching was longer in 35°C than 38°C embryos (23.7 vs. 20.6 days), but unaffected was the relative timing of appearance of developmental landmarks (internal, external pipping). At stage 43-44, 38°C embryos maintained oxygen consumption around 1 mL/g/h despite acute temperature reduction (suggesting thermoregulatory maturation), unlike 35°C embryos. In 35°C embryos the lower oxygen-carrying capacity and temperature insensitive blood O2 affinity (P50 about 30 mmHg) may restrict O2 delivery to tissues, limiting metabolism during decreased ambient temperature. Reduced incubation temperature retards normal hematological and thermoregulatory development.

Le Syndrome WHIM (SW) est un déficit immuno-hématologique rare qui se caractérise notamment par une profonde leucopénie circulante. Le SW résulte principalement de mutations hétérozygotes autosomiques dans CXCR4 qui tronquent partiellement le domaine C-terminal de la protéine et entraînent un défaut de désensibilisation de CXCR4 en réponse à CXCL12. L’impact in vivo d’un gain de fonction de CXCR4 sur le développement et la différenciation lymphocytaires restant à définir, nous avons généré un modèle murin hétérozygote pour la mutation ponctuelle identifiée chez certains patients. L’objectif de ma thèse a été de déterminer si un défaut de différenciation des cellules souches et des progéniteurs hématopoïétiques (CSPH) était à l’origine de ces anomalies de lymphopoïèse. Nous avons observé que le nombre et la clonogénicité des CSH sont préservés dans la moelle osseuse des souris mutantes. Toutefois, les CSH porteuses de la mutation gain de fonction de Cxcr4 ont une quiescence accrue alors que leur multipotence et leur auto-renouvellement sont réduits. Ces dérégulations du compartiment des CSH entraînent, in vivo et in vitro, une altération de la production et de la spécification lymphoïde des progéniteurs multipotents (MPP). En périphérie, nous avons mis en évidence une diminution des CSPH dans le sang des souris mutantes, anomalie également retrouvée à partir de prélèvements sanguins de quatre patients atteints du SW et porteurs d’une mutation hétérozygote dans CXCR4. Ces résultats indiquent une anomalie de circulation des CSPH, hypothèse confortée par le développement d’une hématopoïèse extra-médullaire intra-splénique chez les souris mutantes. Ces données suggèrent que la désensibilisation de Cxcr4 régule la quiescence, la multipotence et l’auto-renouvellement des CSH et serait requise pour la spécification lymphoïde des MPP. Ainsi, l’absence de ce processus pourrait être à l’origine de la lymphopénie observée dans notre modèle murin et, par extrapolation, chez les patients. / The Warts, Hypogammaglobulinemia, Infections and Myelokathexis Syndrome (WS) is a rare immuno-hematological disorder characterized notably by a pan-leukopenia. It is mostly caused by heterozygous autosomal dominant gain-of-function mutations in CXCR4, which engender a distal truncation in the carboxyl-terminal tail domain and lead to a desensitization-resistant receptor. The in vivo impact of a gain-of-CXCR4 function on lymphocyte development and differentiation remain to be established. Then we recently produced major breakthroughs in the pathophysiology of the WS by generating a mouse strain harbouring a WS-linked heterozygous Cxcr4 mutation. The aim of my PhD was to determine if a HSPC differentiation defect would account for the lymphopenia. We showed that the total number and the clonogenicity of HSC were not altered in the BM of mutant mice. However, HSC carrying the gain-of-function mutation in Cxcr4 have an increased quiescence but a decreased multipotency and self-renewal. These HSC compartment deregulations lead to a in vivo and in vitro decline of multipotent progenitors (MPP) production and lymphoid specification. Furthermore, we reported a decrease in the total number of HSPC in the blood of mutant mice, an anomaly also found in four WS patients carrying a heterozygous CXCR4 mutation. This suggested an abnormal HSPC circulation that was strengthened by an enhanced extramedullary haematopoiesis observed in the spleen of mutant mice. These results suggest that Cxcr4 desensitization regulates HSC quiescence, multipotency and self-renewal and is required for MPP lymphoid specification. In this respect, the absence of such process could account for the lymphopenia in this model and likely in patients.

Vitamin B12 and folate are necessary for bone marrow progenitor growth and division. Deficiencies are common in lymphoproliferative disorders due to increased demands of rapidly growing malignant cells. Isolated vitamin B12 deficiency is seen in 13% of these patients and may be their only manifestation. We present the case of vitamin B12 deficiency anemia due to an underlying malignancy that was discovered following supplementation. A 77-year-old nonsmoker female with chronic kidney disease and hypothyroidism presented to her internist with dyspnea, tachycardia and unintentional 7-pound weight loss. Age-appropriate cancer screenings were up-to-date. Physical exam was notable for an overweight female with tachycardia and trace ankle edema bilaterally. Electrocardiogram demonstrated sinus tachycardia. Labs were remarkable for hemoglobin 10.3 mg/dL (12.1 mg/dL one year ago) and serum B12/mL. She was started on intramuscular vitamin B12 supplementation. At her one-month follow-up, she reported debilitating gastrointestinal distress, rash, and fatigue lasting 5-6 days with every vitamin B12 injection. Physical exam was notable for 20-pound weight loss. Labs revealed hemoglobin 9.9 mg/dL despite serum B12 750 pg/mL and worsening kidney function with marked proteinuria. Additional work-up by primary team and subsequent Hematology & Oncology referral demonstrated elevated M-spike on urine protein electrophoresis and abnormal bone marrow biopsy suspicious for lymphoid malignancy. CT abdomen and whole body PET scan revealed increased uptake in the T12 vertebrae and multiple nodal basins consistent with stage IV lymphoma. Biopsy of vertebral body confirmed diffuse large B-cell lymphoma. The patient received one cycle of chemotherapy with R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone). Her course was complicated by pathologic hip fracture requiring hospitalization and surgical repair. The patient died following cardiac arrest in the setting of septic shock from sigmoid colon perforation 7 months from initial presentation. Vitamin B12 and folate play critical roles in nucleic acid synthesis for bone marrow progenitors. Vitamin B12 deficiency arrests cell growth and division, leading to macrocytic anemia and various neuropsychiatric manifestations. It is a common diagnosis with numerous causes: autoantibodies to digestive proteins, poor dietary intake, small bowel malabsorption, etc. Diagnose with low hemoglobin (/dL or 13 mg/dL in non-pregnant women or men, respectively) and mean corpuscular volume >100 fL plus low serum B12 or elevated homocysteine and methyl-malonic acid levels. Replacement is given orally or intramuscularly. Vitamin B12 and folate deficiencies are found in lymphoproliferative disorders due to increased demands of rapidly growing malignant cells. Isolated vitamin B12 deficiency is seen in 13% of patients and may be the only clue. Replacement will not resolve their anemia. Physicians should monitor patients receiving supplementation. If anemia fails to improve or patients experience systemic symptoms, further investigation for lymphoid malignancies is warranted. This patient had dramatic deterioration with acceleration of underlying malignancy following vitamin B12 replacement. We believe supplementation enabled malignant lymphoid precursors to resume cell cycle growth and division. Only one report of vitamin B12 supplementation associated with unmasking a lymphoid malignancy exists in literature. Further research is needed to support whether supplementation can accelerate lymphoid malignancies.

Adult hemoglobin (HbA) is made up of two pairs of globin chains. Some rare mutations of the globin chains can result in high affinity towards hemoglobin molecule thus changing the equilibrium of normal oxygen loading in lungs and the delivery of same to the tissues. Because of change in the affinity to the oxygen these mutations can result in erythrocytosis (polycythemia). Here we discuss a case of Familial Polycythemia likely due to novel hemoglobin variant. 42-year Caucasian male presents to the clinic with high hemoglobin for several years but otherwise denies any symptoms of headache, vision changes, chest pain. He had history of multiple phlebotomies. His past medical history is significant for Polycythemia, PICC line associated clot,Type2 Diabetes, Hypertension, Epidural abscess. Social history is significant for smokeless tobacco but otherwise non-smoker, non-alcoholic, not an IVDA and doesn’t use testosterone. Family history is significant for his sister, her two 14year old daughters and multiple other family members with elevated hemoglobin and undergo phlebotomies, maternal grandfather died of cancer in his 30s. Physical examination is only significant for BMI of 32.46 otherwise no skin discoloration or cyanosis. Laboratory data WBC 9.4 with normal differential, hemoglobin 18.2, hematocrit 55.4, platelet count 180,000, peripheral blood smear was within normal limits, negative for JAK-2, normal erythropoietin, negative for hemochromatosis, Oxygen dissociation p50 of 19 which is low indicating left shifted dissociation curve, hemoglobin electrophoresis HbA: 61.2%, HbA2: 3%, HbF: 0%, Beta variant: 35.8%. In order to further characterize beta variant Bi-directional sequence analysis for Molecular alterations was performed and the following alterations were detected Gene: HBB, DNA change: Codon 39, heterozygous CAG>CCG Protein change: P.G1n39Pro. [glutamine (Q) to proline (P)]. HGVS: c.119A>C, p.Q40P, Classification: Likely deleterious variant. (GenBank accession number NM_000518.4). This is a previously unreported beta chain hemoglobin variant present. This hemoglobin variant is named as Hemoglobin Hyden based on the place where this is found in Hyden, Kentucky. There have been four variants reported at codon 40 of the beta globin gene, which is an external contact site between beta globin and alpha-2 globin. One variant, Hb vassa, is associated with mild hemolytic anemia. The three other variants (Hb Alabama, Hb Tianshui and Hb San Bruno) are not associated with clinical or hematological abnormalities. In our opinion p.Q40P is likely a cause of erythrocytosis. In order to further establish the causality it may be beneficial to test first degree relative to in this family in order to determine whether the p.Q40P alteration tracks with disease and is not present in unaffected individuals. Hemoglobin threshold for phelebotomy to lower the risk of thrombosis and cardiovascular events is yet to be defined.

Through the recruitment of immunologic mechanisms, allogeneic hematopoeitic cell transplantation (HCT) has been establiched as a curative treatment for various hematologic diseases. The most convenient source to obtain hematopoietic progenitor cells are peripheral blood stem cells (PBSCs) which are harvested from the donor via leukapheresis after mobilization with granulocyte-colony stimulating factors. With the introduction of reduced intensity condition (RIC), the curative potential of allogeneic HCT became accessible to older and/or frail patients otherwise ineligible for HCT. However, new challenges arise as the increasing age of patients is inevitably accompanied by a comparable increase in the age of donors. Safety considerations of collecting PBSCs might attain new dimensions. Data to potential risks in elderly donors are lacking. Moreover, the impact of donor’s age on the feasibility of PBSCs collection and on the quality of the harvest in terms of stem cells (CD34+) and natural killer (NK)-cells has not been studied. It is also unknown whether PBSCs obtained from donors above 50 years would negatively influence engraftment or the incidence of graft-versus-host disease (GVHD) in the recipient. These questions were explored in a retrospective study including 167 recipients of an allogeneic HCT (52.7% after RIC) from a matched related sibling. Median donors’ age was 47 years [67 (40%) donors were > 50 years including 34 donors > 60 years]. Safety of mobilization and leukapheresis was age independent. Adequate PBSCs were collected from all donors though a higher CD34+-cell count was seen in donors < 50 years (p<0.0005), whereas harvests from donors > 60 years contained a higher NK-cell count (p=0.003). Engraftment in the recipient occurred after a median of 12 days and was not affected by an advanced donor age. Similarly, a higher incidence of GVHD was not seen in recipients of harvests from older donors. For the first time, we show that donor’s age, even beyond 60 years, does not preclude successful collection of PBSCs from siblings, does not jeopardize the short-term safety of the donor, and is not associated with deleterious sequels for the recipient in terms of engraftment or GVHD. As NK-cells have been implicated in the suppression of GVHD, and the mediation of a graft versus leukemia effect, the impact of the higher number of NK-cells in harvests from elderly donors on relapse of hematologic malignancies in the recipient warrants further studies.

Thousands of parrots all over the world suffer from illnesses and medical complications that can result from interactions between their immune system and bacteria in their digestive tract. The aim of this master's thesis is to understand the link between symptoms of these medical issues, the composition of blood and gastrointestinal microbiota in parrots. Using the hematological methods, 198 blood samples representing 53 parrot species were analyzed. The composition of microbiome was defined by combination of a molecular approach using bacterial 16S rRNA gene sequencing in 132 fecal samples, 12 intestine samples, 228 cloacal swabs and 236 beak swabs representing in total 61 parrot species and a diagnostic approach by psittacine fecal Gram's stain method. Significant association of hematological parameters with individual, environmental and clinical factors was observed, as well as its considerable interspecific variability. Absolute heterophile and lymphocyte counts have been shown more useful for infectious and autoimmune disease monitoring than H/L ratio. Relative numbers of basophiles were the best indicator for behavioral disorders. In relation to hematological parameters, the effect of the bacterial family Flavobacteriaceae, as part of the oral microbiota, and the bacteria Escherichia or...