Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-11T00:01:53Z No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-05-18T23:49:59Z (GMT) No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) / Made available in DSpace on 2016-05-18T23:49:59Z (GMT). No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) Previous issue date: 2015-03-27 / A fam?lia Centropomidae ? composta por tr?s g?neros, Centropomus, Lates e Psammoperca. Centropomus ? o grupo mais diversificado, apresentando seis esp?cies com ocorr?ncia no Oceano Atl?ntico Ocidental, C. undecimalis (Bloch, 1792), C. poeyi Ch?vez, 1961, C. parallelus Poey, 1860, C. mexicanus Bocourt, 1868, C. pectinatus Poey, 1860 e C. ensiferus Poey, 1860. Algumas destas esp?cies s?o consideradas cr?pticas, por apresentarem caracter?sticas morfol?gicas pouco resolutivas para fins de identifica??o. Apesar do grande interesse como recurso natural e para cultivo, aspectos sobre sua diversidade e padr?es cariot?picos s?o pouco conhecidos. Neste trabalho classifica??es morfol?gicas e compara??es de sequ?ncias mitocondriais 16S foram utilizadas para identifica??o das esp?cies do g?nero Centropomus com ocorr?ncia no Rio Grande do Norte, nordeste do Brasil. Duas esp?cies foram identificadas, C. undecimalis e C. mexicanus, que tiveram seus aspectos cromoss?micos analisados atrav?s de m?todos citogen?ticos cl?ssicos (colora??o convencional, bandamento C, Ag-RONs), colora??o com fluorocromos AT- e GC-espec?ficos, bandas de replica??o pela incorpora??o do an?logo de base 5?BrdU ( 5-bromo-2?-deoxiuridina) e mapeamento cromoss?mico in situ de sequ?ncias (TTAGGG)n e dos genes RNAr 18S e 5S. Ambas as esp?cies apresentaram 2n=48 cromossomos acroc?ntricos, com s?tios ribossomais (Ag-RON/DNAr 18S/Mitramicina+) no segundo par cromoss?mico, em posi??o telom?rica no bra?o longo de C. mexicanus) e intersticial em C. undecimalis. O par organizador nucleolar (par 2) se mostra um marcador citotaxon?mico resolutivo para estas duas esp?cies. Os dados gerados revelam uma menor diversidade de esp?cies de Centropomus do que se acreditava, sugerindo uma maior aten??o na identifica??o taxon?mica das esp?cies, tendo em vista otimizar a??es de explora??o comercial, conserva??o biol?gica e cultivo. / The Centropomidae family consists of three genera, Centropomus, Lates and Psammoperca. Centropomus is the most diverse group, with six Centropomus species occur in the Western Atlantic Ocean C. poeyi Ch?vez, 1961, C. parallelus Poey, 1860, C. mexicanus Bocourt, 1868, C. pectinatus Poey, 1860 and C. ensiferus Poey, 1860. Some of these species are considered cryptic, because of its morphological traits showed low resolution for identification purposes. Despite showing great interest as a natural resource and fish culture, aspects of their diversity and karyotypic patterns are poorly understood. In this work morphological identification and comparison of mitochondrial 16S gene sequence were used to identify the species of the genus Centropomus occurring in Rio Grande do Norte, northeastern Brazil. Two sepecies were identified, C. undecimalis and C. mexicanus, which had the chromosomal aspects analyzed, through Classical cytogenetic method analyzes (conventional staining, C-banding, Ag-NORs), fluorochrome staining AT- and GC-specific, replication bands by incorporating of the base analog 5-Bromo-2?-deoxyuridine (5-BrdU), in situ chromosomal mapping of (TTAGGG)n sequences and in situ chromosome mapping 18S and 5S rRNA genes. Both species show 2n=48 acrocentric chromosomes, with ribosomal sites (Ag-NOR/18S rDNA/ Mitramycin+) in second chromosomal pair, in telomeric position on the long arm in C. mexicanus and interstitial in C. undecimalis. The nuclear organization pair (pair 2) shown a resolutive cytotaxonomic marker for these two species. The generated data reveal a lower species diversity than previously believed, suggesting that greater attention should be paid in taxonomic identification of the species, in view of optimize commercial actions exploitation, biological conservation and cultivation.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-15T22:23:03Z No. of bitstreams: 1 Tese-Maria do Socorro.pdf: 2248544 bytes, checksum: 195ed518eb9bcdd7d8346e1af11ce2bc (MD5) / Made available in DSpace on 2016-09-15T22:23:03Z (GMT). No. of bitstreams: 1 Tese-Maria do Socorro.pdf: 2248544 bytes, checksum: 195ed518eb9bcdd7d8346e1af11ce2bc (MD5) Previous issue date: 2015-10-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Brazil is one of the main centers of genetic diversity and dispersion of the genus Passiflora. In the present work, several species and interspecific hybrids of Passiflora were characterized through cytogenetic techniques and ISSR markers, as well as these genotypes were evaluated for in vitro multiplication and establishment. Chromosomal preparations were submitted to conventional staining, CMA3/DAPI banding, FISH using 45S/5S rDNA probes and GISH. Genomic DNA of P. edulis and P. cincinnata were used as genomic probes in the GISH. Twenty ISSR primers were used for the characterization by molecular markers. Nodal segments of P. cincinnata, P. laurifolia, P. setacea and P. luetzelburgii were collected and evaluated in the WPM, DKM and MS culture media to establishment of in vitro culture; for in vitro multiplication, explants were inoculated in DKW culture media under different concentrations of BAP, KIN and 2iP cytokinins. The results showed 2n = 18 chromosomes for the species and hybrids, regular meiosis, one pair of 5S rDNA sites and two or three pairs of 45S rDNA sites co-localized with the CMA3+/DAPI- bands. It is the first chromosomal counting recorded for P. luetzelburgii. Three groups distinct of chromosomes were revealed by GISH. The hybrid origin and the relationship with the parental species were also confirmed by using of the ISSR markers. DKW culture medium provided the better development of the explants, P. cincinnata CPE16 showed the better in vitro morphogenetic response, and KIN showed to be more efficient in obtaining increased shoots length. / O Brasil ? um dos principais centros de dispers?o da variabilidade gen?tica do g?nero Passiflora. O presente trabalho objetivou caracterizar esp?cies e h?bridos interespec?ficos de Passiflora, atrav?s de t?cnicas citogen?ticas e por marcadores ISSR, al?m de avaliar o estabelecimento e multiplica??o in vitro de gen?tipos de maracujazeiro. Prepara??es citol?gicas foram submetidas ? an?lise convencional, dupla colora??o com os fluorocromos CMA3/DAPI, FISH utilizando como sonda DNAr 5S/45S e GISH. O DNA gen?mico de P. edulis e P. cincinnata foram utilizados como sondas gen?micas na GISH. Foram utilizados 20 primers ISSR na caracteriza??o por marcadores moleculares. Para o estabelecimento do cultivo in vitro, segmentos nodais de P. cincinnata, P. laurifolia, P. setacea e P. luetzelburgii foram coletados e avaliados em meio de cultura WPM, DKW e MS, para a multiplica??o in vitro, os explantes foram inoculados em meio DKW sob diferentes concentra??es de citocininas BAP, KIN e 2iP. Os resultados mostraram 2n = 18 cromossomos para as esp?cies e h?bridos, al?m de meiose regular, um par de s?tios de DNAr 5S e dois a tr?s pares de s?tios de DNAr 45S que co-localizaram as bandas CMA3+/DAPI-, sendo o primeiro registro de contagem para P. luetzelburgii. Na GISH foi observada a forma??o de tr?s grupos cromoss?micos distintos. A origem h?brida e a rela??o com as esp?cies parentais foram tamb?m confirmadas pelo uso dos marcadores ISSR. No cultivo in vitro, o meio DKW proporcionou melhor desenvolvimento dos explantes, em rela??o aos gen?tipos, P. cincinnata CPE16 mostrou melhor resposta morfog?nica in vitro e KIN mostrou-se mais eficiente para obten??o de brota??es com maiores comprimentos.