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Despite formidable advances in virology and medicine in recent decades, we know remarkably little about the dynamics of the influenza virus in the environment during transmission between hosts. There is still controversy over the relative importance of various transmission routes, and the seasonality of influenza remains unexplained. This work focuses on developing new knowledge about influenza transmission via the airborne route and the virus' inter-host dynamics in droplets and aerosols. We measured airborne concentrations of influenza A viruses (IAVs) and size distributions of their carrier aerosols in a health center, a daycare center, and airplanes. Results indicate that the majority of viruses are associated with aerosols smaller than 2.5 µm and that concentrations are sufficient to induce infection. We further modeled the fate and transport of IAV-laden droplets expelled from a cough into a room, as a function of relative humidity (RH) and droplet size. The model shows that airborne concentrations of infectious IAV vary with RH through its influence on virus inactivation and droplet size, which shrinks due to evaporation. IAVs associated with large droplets are removed mostly by settling, while those associated with aerosols smaller than 5 µm are removed mainly by ventilation and inactivation. To investigate the relationship between RH and influenza transmission further, we measured the viability of IAV in droplets at varying RHs. Results suggest that there exist three regimes defined by RH: physiological conditions (~100% RH) with high viability, concentrated conditions (~50% to ~99% RH) with lower viability, and dry conditions (<~50% RH) with high viability. A droplet's extent of evaporation, which is determined by RH, affects solute concentrations in the droplet, and these appear to influence viability. This research considerably advances the current understanding of the dynamics of the influenza virus while it is airborne and provides an explanation for influenza's seasonality. Increased influenza activity in winter in temperate regions could be due to greater potential for IAV carrier aerosols to remain airborne and higher viability of IAV at low RH. In tropical regions, transmission could be enhanced due to better survival of IAV at extremely high RH. / Ph. D.

This dissertation focuses on the design, synthesis, characterization of platinum (II) pyridylazolate complexes and develop high performance organic light emitting diodes (OLEDs) and design and execute high-sensitivity humidity sensors based on the luminescent metal-organic complexes of platinum. A majority of existing platinum compounds do not dissolve in organic solvents, making it difficult to analyze the photophysical characteristics of complexes in solution, a key part of understanding chemical photophysical properties. Furthermore, due to the poor quantum yield, it is inefficient for use in devices such as OLEDs. Chapter 2 reports the synthesis and characterization of a novel heteroleptic platinum(II) pyridylazolate complex with high solubility and quantum yield. The photochemistry of the complex is studied, including efficiency, emission profiles, and lifetimes at different temperatures. Chapter 3 reports the power efficiency (lm/W), current efficiency (cd/A), external quantum efficiency (EQE), luminance and operating voltage (V) of OLED devices made with the heteroleptic platinum(II) pyridylazolate complex. The relation between thickness of hole transport layer and electron transport layer on performance of devices has been studied through building a variety of devices. Chapter 4 includes application of a homoleptic platinum(II) pyridylazolate complex in humidity sensor. In many environments, the relationship between moisture content and emissive wavelength has been investigated. This research reveals that regardless of the humidity level, there is a link between increasing the temperature and decreasing the moisture absorption capacity of the complex.

The first objective of this problem - thorough literature review - was conducted on all available references concerning humidity measurement, and visits were made to the American Instrument Company in Silver Springs, Maryland, and the National Bureau of Standards. The survey evaluated the instruments in use today and also explored many of the ideas and concepts that have been suggested in past years as possible means for humidity measurement. A summation of the survey indicated that the electric hygrometer with a ceramic element was a good basic theory for a humidity instrument, but still required development before it,would be of any practical use. The second objective - development of a useful hygrometer - was directed towards the development of the electric hygrometer using a ceramic element. The study that resulted included the evaluation of two methods for determining the change of resistance of the ceramic element, as well as a preliminary investigation of the characteristics of a ceramic element when employed as the sensing element for an electric hygrometer. The element intended for the initial tests, a porous ceramic containing mostly aluminum oxide, was the only material tested, but the tests included elements of the pure ceramic material and the same material after it was soaked in a saturated solution of lithium chloride. With the instruments used, the indications were that both elements were suited for determining the high humidities. The pure element had a lower limit of 50 percent relative humidity, and the coated element a lower limit of 20 percent. Two instruments were used for indicating the change of relative humidity. A vacuum tube voltmeter type instrument was tested, but found unsatisfactory due to variations of readings under the same conditions, drifting of the zero set, and excessive fluctuations of the indicating meter. The other instrument or method used was to apply an A-C voltage across the element, and to measure the resulting current flow. Because the current was maintained above 1 milliampere, heating of the element was caused, resulting in a change of element resistance. However, results with this instrument were sufficient to establish a calibration curve for the apparatus. A comparison of this device and a psychrometer indicated the usefulness of such an instrument, but confirmed that the development was not complete. The overall result of the research indicates that the selection of the ceramic sensing element for an electric hygrometer has merit, but more development is necessary. Two phases of any future work on the problem should be the development of the indicating instrument, and the selection and design of the sensing element. The need for a satisfactory instrument still exists, and the successful development of the type instrument described herein, would undoubtedly meet the requirements. / Master of Science

The transmission of some infectious diseases requires that pathogens can survive (i.e., remain infectious) in the environment, outside the host. The viability of pathogens that are immersed in aerosols and droplets is affected by factors such as relative humidity (RH) and the chemical composition of the liquid media, but the effects of these stressors on the viability of viruses have not been extensively studied. The overall objective of this work was to investigate the effects of RH and media composition on the viability of viruses in suspended aerosols and stationary droplets. We used a custom rotating drum to study the viability of airborne 2009 pandemic influenza A(H1N1) virus across a wide range of RHs. Viruses in culture medium supplemented with material from the apical surface of differentiated primary human airway epithelial cells remained equally infectious for 1 hour at all RH levels tested. We further investigated the viability of two model viruses, MS2 and Φ6, in suspended aerosols and stationary droplets consisting of culture media. Contrary to the results for influenza virus, we observed a U-shaped viability pattern against RH, where viruses retained their viability at low and extreme high RHs, but decayed significantly at intermediate to high RHs. By characterizing the droplet evaporation kinetics, we demonstrated that RH mediated the evaporation rate of droplets, induced changes in solute concentrations, and modulated the cumulative dose of solutes to which viruses were exposed as droplets evaporated. We proposed that the decay of viruses in droplets follows disinfection kinetics. Lastly, we manipulated the chemical composition of media to explore the stability of viruses as a function of pH and salt, protein, and surfactant concentrations. Results suggested that the effects of salt and surfactant were RH and strain-dependent. Acidic and basic media effectively inactivated enveloped virus. Protein had protective effect on both non-enveloped and enveloped viruses. Results from this work has advanced the understanding of virus viability in the environment and has significant implications for understanding infectious disease transmission. / Doctor of Philosophy / Pathogenic organisms, including bacteria, viruses, fungi, protozoa, and helminths, cause infections that are responsible for substantial morbidity and/or mortality. For example, it is estimated that influenza has caused 9 million to 45 million illnesses and 12,000 to 61,000 deaths annually since 2010 in the United States. The spread of certain diseases relies on people touching the pathogenic organism on surfaces or inhaling it from the air. Successful transmission requires that the pathogen survive, or maintain its infectivity, while it is in the environment. The survival of pathogens can be affected by temperature, humidity, composition of the respiratory fluid carrying them, and other factors. However, there is limited research investigating the effects of these factors on the survival of viruses in the environment. In this work, we studied the effect of relative humidity (RH) on the survival of viruses, including influenza virus and two other types of viruses, in inhalable aerosols and larger droplets. We found that influenza viruses survive well in aerosols across a wide range of RH levels for at least 1 h. Conversely, the two model viruses survived best at both low and very high RHs, such as found indoors in the wintertime or in tropical regions, respectively, but had a pronounced decay at intermediate RHs. By measuring how fast droplets evaporated, we found that RH affected their chemistry and determined the total amount of stress that viruses were exposed to. This explained why a "U-shaped" survival pattern was observed against RH. We also investigated the survival of viruses in droplets containing different components. Results indicated that the effects of salt, surfactant, protein, and droplet pH depended on RH and the type of virus. The outcomes of this work are meaningful in predicting the survival of viruses in aerosols and droplets of various compositions in the environment and could provide insight on developing strategies to minimize the spread of infectious diseases.

The diploma thesis is focused on the issue of measuring the moisture content in building materials using indirect methods that allow its long-term measurement. The theoretical part of the thesis provides an overview of the forms of moisture in porous building materials and provide an overview of the principles of measuring moisture content. In the experimental part of the thesis, the aim is to verify the available methods for long-term monitoring of moisture in building materials built into building structures. Proven methods of measuring humidity include resistance, capacitive and microwave methods and their comparison with the results of gravimetric measurements. At the end of the work is an evaluation of knowledge and recommendations for further research in this area.

This thesis compares the methods for the determination of air and moisture condensation in the construction. There are descriptions of the various methods and formulas for measuring humidity and methods for the calculation of condensation in building structures. The practical part describes the development of a method for verifying the condensation of moisture in construction. Parts of this work are the materials and methods for the development of the humidity sensor. There were used two methods – capacitive and resistive for measuring moisture condensation. In the conclusion is the evaluation of the humidity sensor and compares the results of individual measurements.

Rotary air-to-air energy exchangers (also called energy wheels) transfer both heat and moisture between supply and exhaust airstreams in buildings. In this thesis, it is hypothesized that the transient step response characteristics of an energy wheel are uniquely related to the steady-state cyclic response of the wheel. The primary objective of this research is to study the transient response of a humidity/temperature sensor and measure energy wheel performance with a new test procedure that uses only transient response characteristics. In this thesis, the transient characteristics of a humidity/temperature sensor and an energy wheel to a step change in relative humidity and temperature are investigated through two types of measurements. One test uses a small airflow, at controlled temperature and humidity conditions, passing through a small section of a porous wheel while measuring the outlet conditions after the inlet conditions are suddenly changed. For a step input, it is shown that the outlet humidity/temperature sensor data correlate with an exponential function with two time constants. Since the transient response characteristics of the humidity/temperature sensor must be known to predict the response of the wheel alone, a second test is required that is similar to the first test except that the wheel is removed. This test is used to obtain the transient response of the sensor alone. Data from these tests show that both the sensor and the sensor plus wheel have two sets of two time constants. An analysis is presented to determine the transient response of the wheel alone using the correlated properties of the sensor alone and the sensor with a wheel upstream. The challenge undertaken in this research was the development of a more flexible, lower cost test facility than that presented in ASHRAE Standard 84-1991(Method of Testing Air-to-Air Heat Exchangers). In future work, this new laboratory experimental test facility should be adapted to test most types of energy wheels. The configuration allows a wide range of mass flow rates, inlet supply air temperatures and relative humidities. Uncertainty analysis is used for each transient test for the sensors and air-to-air energy wheels to specify the sensor and wheel plus sensor characteristics. This uncertainty analysis shows that accurate sensor calibration under equilibrium conditions and the start time for the humidity sensor step change is crucial to achieve low uncertainties in the transient behaviour of sensor and energy wheels. Knowing the uncertainty in the characteristics of the sensors and the wheel plus sensors the uncertainty in the transient response of the wheel alone is predicted. The first time constant of the humidity sensor is found to be about 3 seconds, while the second time constant is found to be about 100 seconds. It is found that the predicted response of the wheel alone gives time constants that are about 6 seconds and 140 seconds. Other researchers can use this information presented in this thesis to estimate the effectiveness of an energy wheel.

Rotary air-to-air energy exchangers (also called energy wheels) transfer both heat and moisture between supply and exhaust airstreams in buildings. In this thesis, it is hypothesized that the transient step response characteristics of an energy wheel are uniquely related to the steady-state cyclic response of the wheel. The primary objective of this research is to study the transient response of a humidity/temperature sensor and measure energy wheel performance with a new test procedure that uses only transient response characteristics. In this thesis, the transient characteristics of a humidity/temperature sensor and an energy wheel to a step change in relative humidity and temperature are investigated through two types of measurements. One test uses a small airflow, at controlled temperature and humidity conditions, passing through a small section of a porous wheel while measuring the outlet conditions after the inlet conditions are suddenly changed. For a step input, it is shown that the outlet humidity/temperature sensor data correlate with an exponential function with two time constants. Since the transient response characteristics of the humidity/temperature sensor must be known to predict the response of the wheel alone, a second test is required that is similar to the first test except that the wheel is removed. This test is used to obtain the transient response of the sensor alone. Data from these tests show that both the sensor and the sensor plus wheel have two sets of two time constants. An analysis is presented to determine the transient response of the wheel alone using the correlated properties of the sensor alone and the sensor with a wheel upstream. The challenge undertaken in this research was the development of a more flexible, lower cost test facility than that presented in ASHRAE Standard 84-1991(Method of Testing Air-to-Air Heat Exchangers). In future work, this new laboratory experimental test facility should be adapted to test most types of energy wheels. The configuration allows a wide range of mass flow rates, inlet supply air temperatures and relative humidities. Uncertainty analysis is used for each transient test for the sensors and air-to-air energy wheels to specify the sensor and wheel plus sensor characteristics. This uncertainty analysis shows that accurate sensor calibration under equilibrium conditions and the start time for the humidity sensor step change is crucial to achieve low uncertainties in the transient behaviour of sensor and energy wheels. Knowing the uncertainty in the characteristics of the sensors and the wheel plus sensors the uncertainty in the transient response of the wheel alone is predicted. The first time constant of the humidity sensor is found to be about 3 seconds, while the second time constant is found to be about 100 seconds. It is found that the predicted response of the wheel alone gives time constants that are about 6 seconds and 140 seconds. Other researchers can use this information presented in this thesis to estimate the effectiveness of an energy wheel.