Hydrogels based on self-assembling peptides are believed to have potential for use in a wide range of biomedical and biodiagnostic applications. For many of these, control over various properties of the gels is essential for tuning the gels to fit certain constraints or requirements in terms physical properties such as diffusive properties and swelling. One important property to control for applications such as cell culture and drug delivery is its mechanical strength, and this study investigates three different strategies by which the individual peptide monomers can be modified in order to effect a change in the macromolecular self-assembled structure and therefore a bulk change in the mechanical stiffness. In chapter 4, two ionic-complementary octapeptides, FEFKFKFK and FEFQFKFK are described, with monomer charges of +2 and +1, respectively at physiological pH. FEFKFKFK was observed to form largely discrete fibrils, characteristic of similar systems, while FEFQFKFK formed fibril bundles – believed to be a limited form of an aggregation effect frequently seen in similar peptides with neutral charge. As a result of this structural change, FEFQFKFK was found to have values for the elastic and viscous moduli (which are often used to measure the ‘strength’ of a gel) between 5 and 10 times larger than those of FEFKFKFK at the same concentration. The same behaviour was seen in FEFKFKFK when the monomer charges were reversed by adjusting pH, suggesting that the monomer charge is indeed responsible for the bundling effect. In chapter 5, two branched peptides were designed and synthesized: KG17, with two arms consisting of self-assembling FKFEFKFK-motifs, and KG28 which had three such arms. Each branched peptide was doped into pure FKFEFKFK and the resulting gels investigated. While no obvious structural changes were observed for either dopant (save for a potential fibril parallelisation effect with KG17 observed in Small-Angle Neutron Scattering (SANS)), both were observed to increase the elastic and viscous moduli of the gels at overall peptide concentrations of 30 and 50 mg mL-1 (gels), but not at 10 mg mL-1 (viscous liquid). The most dramatic change was observed in the 50 mg mL-1 gels, suggesting that higher concentrations could enhance the effect of the dopants. In chapter 6, three thermo-responsive polymers (pTEGMA), of Degrees of polymerisation (DPs) 17, 47 and 142 were conjugated to CGFKFEFKFK and incorporated into a peptide hydrogel. Gels containing the non-conjugated versions of each polymer were also tested. While no changes in morphology were observed at the fibillar level, the polymer Lower Critical Solution Temperature (LCST) behaviour could be observed in SANS in all samples apart from the DP17 conjugate. However, in rheological tests gels doped with this conjugate appeared to show the strongest the elastic and viscous moduli. In general the conjugates appeared to increase the elastic and viscous moduli, particularly at temperatures above ca. 50°C. Rather than this being LCST behaviour, it was suggested that the polymers can act to enhance a natural thermo-response that was observed in the peptide, with the shortest polymer (DP17) experiencing the least steric hindrance and therefore having the strongest effect. It was postulated that this interaction could involve the screening of charge on the peptide fibril. Non-conjugated polymer appeared to have little effect on the mechanical properties, with elastic modulus values correlating strongly to the overall peptide concentration.

Plasma polymerization techniques were used to synthesize and deposit hydrogel on silicon (Si) substrate. Hydrogel is a network of polymer chains that are water-insoluble and has a high degree of flexibility. The various fields of applications of hydrogel include drug release, biosensors and tissue engineering etc. Hydrogel synthesized from different monomers possess a common property of moisture absorption. In this work two monomers were used namely 1-amino-2-propanol (1A2P) and 2(ethylamino)ethanol (2EAE) to produce polymer films deposited on Si ATR crystal. Their moisture uptake property was tested using FTIR-ATR technique. This was evident by the decrease in -OH band in increasing N2 purging time of the films. Secondly, two monomer compounds namely vinyl acetic acid and glycidyl methacrylate which have both amine and carboxylic groups are used as solid surface for the immobilization of bovine serum albumin (BSA). Pulsed plasma polymerization was used to polymerize these monomers with different duty cycles. Initial works in this field were all about protein surface adsorption. But more recently, the emphasis is on covalent bonding of protein on to the surface. This immobilization of protein on solid surface has a lot of applications in the field of biochemical studies. The polymerization of vinyl acetic acid and glycidyl methacrylate were shown as successful method to attach protein on them. Chemical mechanical polishing (CMP) of Cu is one of the processes in the integrated chips manufacturing industry. Benzotriazole is one of the constituents of this CMP slurry used as corrosion inhibitor for Cu. Benzotriazole (C6H5N3) is a nitrogen heterocyclic derivative having three nitrogen atoms, each with an unshared pair of electrons, forming five-membered ring structure. This molecule coordinates with Cu atoms by loosing a proton from one of its nitrogen atom and thereby forming a film which is polymeric in nature that prevents further oxidation of Cu. In this work, aqueous solution of BTA was used to study the adsorption characteristics on Cu when a Cu electrodeposited Si was immersed in to it. This study was performed using ATR-FTIR technique. The adsorption of BTA on Cu surface was evident by the presence of aryl C-H stretching band at 3061 cm-1.

Homogeneous hydrogels made of an interpenetrating network of poly(N-isopropylacrylamide) (PNIPAm) and poly(acrylic acid) (PAAc) are synthesized by a two-step process; first making PNIPAm hydrogels and then interpenetrating acrylic acid throughout the hydrogel through polymerization. The kinetic growth of the IPN is plotted and an equation is fitted to the data. When diluted to certain concentrations in water, the hydrogels show reversible, inverse thermal gelation at about 34°C. This shows unique application to the medical field, as the transition is just below body temperature. A drug release experiment is performed using high molecular weight dyes, and a phase diagram is created through observation of the purified, concentrated gel at varying concentrations and temperatures.

This thesis presents a novel ZnO-hydrogel based fluorescent colloidal semiconductor nanomaterial system for potential bio-medical applications such as bio-imaging, cancer detection and therapy. The preparation of ZnO nanoparticles and their surface modification to make a biocompatible material with enhanced optical properties is discussed. High quality ZnO nanoparticles with UV band edge emission are prepared using gas evaporation method. Semiconductor materials including ZnO are insoluble in water. Since biological applications require water soluble nanomaterials, ZnO nanoparticles are first dispersed in water by ball milling method, and their aqueous stability and fluorescence properties are enhanced by incorporating them in bio-compatible poly N-isopropylacrylamide (PNIPAM) based hydrogel polymer matrix. The optical properties of ZnO-hydrogel colloidal dispersion versus ZnO-Water dispersion were analyzed. The optical characterization using photoluminescence spectroscopy indicates approximately 10 times enhancement of fluorescence in ZnO-hydrogel colloidal system compared to ZnO-water system. Ultrafast time resolved measurement demonstrates dominant exciton recombination process in ZnO-hydrogel system compared to ZnO-water system, confirming the surface modification of ZnO nanoparticles by hydrogel polymer matrix. The surface modification of ZnO nanoparticles by hydrogel induce more scattering centers per unit area of cross-section, and hence increase the luminescence from the ZnO-gel samples due to multiple path excitations. Furthermore, surface modification of ZnO by hydrogel increases the radiative efficiency of this hybrid colloidal material system thereby contributing to enhanced emission.

Orientador: Marisa Masumi Beppu / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química / Made available in DSpace on 2018-08-24T10:04:09Z (GMT). No. of bitstreams: 1 Moraes_MarianaAgostinide_D.pdf: 3647517 bytes, checksum: a0e6d1b53849e9a302a2f7d322f2e8b7 (MD5) Previous issue date: 2014 / Resumo: Neste estudo, a fibroína de seda foi explorada no preparo de materiais micro e nanoestruturados, em estruturas bi- e tridimensionais, visando o uso em biomateriais. Para isto, foi estudada a incorporação de microfibras de fibroína em hidrogéis de colágeno, formando uma estrutura 3D, e a formação de filmes ultrafinos contendo fibroína pelo método layer-by-layer. No primeiro caso, a fibroína de seda foi explorada como um material promissor para reforçar mecanicamente os hidrogéis de colágeno devido às suas ótimas propriedades mecânicas e também por apresentar citocompatibilidade adequada com diversos tipos de células. Os resultados mostraram uma boa interação entre as microfibras de fibroína e as fibrilas de colágeno, além de melhorias na resistência mecânica dos hidrogéis. Os hidrogéis contendo fibroína foram capazes não somente de manter o desempenho biológico do colágeno, mas de melhorar a proliferação celular. Os resultados obtidos sugerem que hidrogéis de colágeno podem ser microestruturados com fibroína de seda e representam um material promissor para uso em engenharia tecidual vascular. Já para o caso dos filmes ultrafinos (2D), foi estudada a formação dos mesmos através do método conhecido como layer-by-layer, pela técnica de imersão de substrato em solução polimérica. Como pares catiônicos foram estudados a quitosana e o hidrocloreto de poli(alilamina), sendo possível controlar a formação de fibrilas nanométricas de fibroína de seda através da variação do par polimérico. Fibrilas alinhadas de fibroína foram observadas no filme layer-by-layer com quitosana, porém no filme com hidrocloreto de poli(alilamina) não foram observadas fibrilas. A densidade de cargas do par polimérico é a característica que governa a formação de fibrilas na escala nanométrica. A etapa de secagem entre cada camada também é fundamental para a formação e estabilização dos filmes, que apresentaram coexistência de conformação molecular seda I e seda II. A viabilidade celular permaneceu entre 90 e 100% em todos os filmes estudados, porém o padrão de topografia imposto pelas fibrilas de fibroína não orientou o crescimento e adesão celular. Filmes finos multicamadas nanoestruturados com fibroína de seda possuem aplicação potencial na área biomédica, como recobrimentos e superfícies funcionais / Abstract: In this study, silk fibroin was explored in the preparation of micro and nanostructured materials, in bi- and tridimensional structures, aiming the use in the biomaterials field. For that, the incorporation of fibroin microfibers in collagen hydrogels forming a 3D structure was studied. We also studied the formation of ultrathin films containing fibroin by the layer-by-layer method. In the first case, the silk fibroin was explored as a promising material to mechanically reinforce collagen hydrogels due to its good mechanical properties and also for having cytocompatibility adequate with several cell types. The results showed a good interaction between fibroin microfibers and collagen fibrils, as well as improvements in the mechanical resistance of the hydrogels. The hydrogels containing fibroin were capable to maintain the biological behavior of collagen and also to improve cell proliferation. The results suggest that collagen hydrogels can be microstructured with silk fibroin and represent a promising material for use in vascular tissue engineering. In the case of the ultrathin films (2D), the formation of these films was studied by the layer-by-layer method, using the technique of substrate dipping in a polymeric solution. Chitosan and poly (allylamine hydrochloride) were used as cationic pair species, being possible to control the formation of nanometric fibrils of fibroin by varying the polymeric pair. Fibroin aligned fibrils were observed in the layer-by-layer film with chitosan, while no fibrils were observed in the film with poly (allylamine hydrochloride). The charge density of the polymeric pair is the main characteristic that governs the fibrils formation at the nanometric scale. The drying step between each layer is also essential for formation and stabilization of the films that show the co-existence of molecular conformation of silk I and silk II. Cell viability between 90 and 100% was attained for all the studied films; however the topographic pattern imposed by the fibroin fibrils did not orient cell adhesion and growth. Multilayer thin films nanostructured with silk fibroin have potential for application in the biomedical field, as coatings and functional surfaces / Doutorado / Engenharia Química / Doutora em Engenharia Quimica

Orientador: Nelson Eduardo Durán Caballero / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Química / Made available in DSpace on 2018-08-27T13:02:52Z (GMT). No. of bitstreams: 1 Bueno_CamilaPedrosoSilveira_M.pdf: 80017692 bytes, checksum: afdb5714b60ba879bf69cbd031077a1d (MD5) Previous issue date: 2015 / Resumo: Um dos principais problemas da quimioterapia, atualmente, é o uso de fármacos extremamente citotóxicos, cuja falta de especificidade acarreta na ação sob todos os tipos de células, incluindo as saudáveis, gerando efeitos colaterais intensos, que prejudicam a terapia em si. Nesse sentido, os nanomateriais oferecem uma opção terapêutica valiosa, pois podem conjugar especificidade e liberação sustentada do fármaco, prevenindo-o de ser degradado prematuramente e permitindo o uso de menores doses. A fim de desenvolver um sistema do tipo drug depot que servisse como plataforma para a liberação sustentada de fármacos, foram produzidas, neste trabalho, formulações baseadas em nanopartículas mesoporosas de sílica e hidrogel de Pluronic F-127, associadas a dois fármacos individualmente: doxorrubicina e sildenafila. As nanopartículas mesoporosas de sílica são boas alternativas para uso médico, pois são biocompatíveis e possuem grande volume de poros, atuando como carreadoras, promovendo a liberação sustentada do fármaco. O Pluronic F-127 atua como agente gelificante e promove uma liberação prolongada das nanopartículas e do fármaco incorporado, uma vez que é um polímero termo-reversível que permite a formação de um hidrogel à temperatura ambiente. A doxorrubicina é um antibiótico antitumoral de largo espectro, amplamente usada no tratamento de diversos cânceres, que atua ligando-se ao DNA e induzindo apoptose celular. Por sua vez, a sildenafila faz parte de uma classe de inibidores da enzima fosfodiesterase-5, cuja expressão aparece aumentada em diversos carcinomas e, por isso, seu papel na progressão tumoral vem sendo amplamente estudado. Após estudar os aspectos físico-químicos envolvendo os componentes dos sistemas, como estabilidade coloidal e interações entre as nanopartículas e biomoléculas presentes no meio biológico, as formulações foram testadas contra câncer de próstata quimicamente induzido em ratos. Os resultados mostram que as nanopartículas possuem um papel determinante no quadro de melhora de tumor, minimizando, também, a toxicidade do antitumoral / Abstract: One of the largest problems of chemotherapy currently is the use of highly cytotoxic drugs with lack of specificity, thus damaging all kinds of tissues, including healthy cells. This leads to aggressive side effects that jeopardize the therapy itself. In this context, nanomaterials emerge as a valuable therapeutic option, as they can provide the specificity to target tumor tissues and a sustained release of the drug, preventing the drugs premature degradation and allowing lower doses. Aiming to develop a drug depot system that would act as platform for the sustained release of drugs, this project proposed to develop formulations based on mesoporous silica nanoparticles and Pluronic F-127 hydrogels, associated with two drugs individually: doxorubicin and sildenafil. The mesoporous silica nanoparticles are good alternatives for biomedical use because of their biocompatible features and their great pore volume, acting as carriers and providing sustained release. Pluronic F-127 acts as the gelling agent and provides a prolonged release of the nanoparticles and the loaded drug, since it is a thermo-reversible polymer that allows the formation of a hydrogel at room temperature. Doxorubicin is an antibiotic and antitumor of large spectra, largely used to treat several types of cancer and acts binding the DNA and inducing cell apoptosis. Sildenafil, in turn, is part of a class of inhibidors of the enzyme phosphodiesterase-5, which expression is increased in many carcinomas and which role in tumor progression has been largely studied. After studying physico-chemical aspects involving the systems produced, such as the colloidal stability, release profile, and the interactions of the nanoparticles with the biomolecules present in biological medium. The formulations were also tested in rats with chemically induced prostate cancer. The results show that the nanoparticles play a determinant role on the improvement of tumor conditions, also reducing doxorubicin's toxicity / Mestrado / Físico-Química / Mestra em Química

Made available in DSpace on 2016-01-26T18:56:02Z (GMT). No. of bitstreams: 1 Murilo Mendes de Angelo.pdf: 1443900 bytes, checksum: f6d656f910c3210fd63e015a55ec60a4 (MD5) Previous issue date: 2014-11-28 / The Agriculture in the Pontal do Paranapanema region is a major source of employment and income, with the highlight the cultivation of sugar cane. Along with the expansion of the cultivated area has been a substantial increase in the use of herbicides. Among the various existing herbicides in the domestic market, glyphosate, Roundup®, appears with great relevance in the agricultural scenario, because in addition to efficient has a broad spectrum of action. Moreover, their use also generates unwanted environmental impacts, such as changes in soil microbes and the generation of a taproot. Additionally, by leaching a part of glyphosate applied to crops can reach surface water, such as rivers. In the face of this problem, this study followed the process of degradation of glyphosate in a biological reactor, through the activated sludge process. Two studies, the first of which used a synthetic wastewater containing glyphosate as a source of carbon, nitrogen and phosphorus and sewage sludge. The second treatment was done under the same conditions first by adding a hydrogel polymer. These polymers are employed in the sugarcane plantations for water retention and can reach surface waters through leaching. The monitoring system was conducted through analysis of chemical oxygen demand (COD), amount of phosphorus, nitrogen, glyphosate, nitrite and nitrate, reaction temperature, pH and Dissolved Oxygen. The results demonstrate that the treatment of the effluent containing only glyphosate is more efficient than treatment containing glyphosate and the hydrogel polymer. / A agricultura na região do Pontal do Paranapanema é uma das principais fontes de emprego e de renda, tendo como destaque o cultivo da cana de açúcar. Junto com a expansão da área cultivada tem ocorrido um aumento substancial na utilização de herbicidas. Dentre os vários herbicidas existentes no mercado nacional, o glifosato, Roundup®, aparece com grande relevância no cenário agrícola, pois além de eficiente possui um amplo espectro de ação. Por outro lado, a sua utilização também acaba gerando impactos ambientais indesejados, como a alteração na microbiota do solo e a geração da raiz pivotante. Adicionalmente, através da lixiviação, uma parte do glifosato aplicado nas lavouras pode atingir as águas superficiais, como por exemplo a dos rios. Em face deste problema, o presente trabalho acompanhou o processo de degradação do glifosato, em um reator biológico, por meio do processo de lodos ativados. Dois estudos foram realizados, sendo que o primeiro utilizou um efluente sintético contendo glifosato como fonte de carbono, nitrogênio e fósforo e o lodo de esgoto. O segundo tratamento foi realizado sob as mesmas condições do primeiro, adicionando-se um polímero hidroabsorvente. Estes polímeros são empregados nas plantações canavieiras para a retenção da água e também podem atingir as águas superficiais, através da lixiviação. O monitoramento do sistema foi realizado através das análises da demanda química de oxigênio (DQO), quantidade de fósforo, de nitrogênio, do glifosato, do nitrito e do nitrato, temperatura do reator, pH e Oxigênio Dissolvido. Os resultados demostraram que o tratamento do efluente contendo apenas o glifosato é mais eficiente do que o tratamento contendo o glifosato e o polímero hidroabsorvente.

Made available in DSpace on 2016-07-18T17:46:20Z (GMT). No. of bitstreams: 1 Murilo Mendes de Angelo.pdf: 1443900 bytes, checksum: f6d656f910c3210fd63e015a55ec60a4 (MD5) Previous issue date: 2014-11-28 / The Agriculture in the Pontal do Paranapanema region is a major source of employment and income, with the highlight the cultivation of sugar cane. Along with the expansion of the cultivated area has been a substantial increase in the use of herbicides. Among the various existing herbicides in the domestic market, glyphosate, Roundup®, appears with great relevance in the agricultural scenario, because in addition to efficient has a broad spectrum of action. Moreover, their use also generates unwanted environmental impacts, such as changes in soil microbes and the generation of a taproot. Additionally, by leaching a part of glyphosate applied to crops can reach surface water, such as rivers. In the face of this problem, this study followed the process of degradation of glyphosate in a biological reactor, through the activated sludge process. Two studies, the first of which used a synthetic wastewater containing glyphosate as a source of carbon, nitrogen and phosphorus and sewage sludge. The second treatment was done under the same conditions first by adding a hydrogel polymer. These polymers are employed in the sugarcane plantations for water retention and can reach surface waters through leaching. The monitoring system was conducted through analysis of chemical oxygen demand (COD), amount of phosphorus, nitrogen, glyphosate, nitrite and nitrate, reaction temperature, pH and Dissolved Oxygen. The results demonstrate that the treatment of the effluent containing only glyphosate is more efficient than treatment containing glyphosate and the hydrogel polymer. / A agricultura na região do Pontal do Paranapanema é uma das principais fontes de emprego e de renda, tendo como destaque o cultivo da cana de açúcar. Junto com a expansão da área cultivada tem ocorrido um aumento substancial na utilização de herbicidas. Dentre os vários herbicidas existentes no mercado nacional, o glifosato, Roundup®, aparece com grande relevância no cenário agrícola, pois além de eficiente possui um amplo espectro de ação. Por outro lado, a sua utilização também acaba gerando impactos ambientais indesejados, como a alteração na microbiota do solo e a geração da raiz pivotante. Adicionalmente, através da lixiviação, uma parte do glifosato aplicado nas lavouras pode atingir as águas superficiais, como por exemplo a dos rios. Em face deste problema, o presente trabalho acompanhou o processo de degradação do glifosato, em um reator biológico, por meio do processo de lodos ativados. Dois estudos foram realizados, sendo que o primeiro utilizou um efluente sintético contendo glifosato como fonte de carbono, nitrogênio e fósforo e o lodo de esgoto. O segundo tratamento foi realizado sob as mesmas condições do primeiro, adicionando-se um polímero hidroabsorvente. Estes polímeros são empregados nas plantações canavieiras para a retenção da água e também podem atingir as águas superficiais, através da lixiviação. O monitoramento do sistema foi realizado através das análises da demanda química de oxigênio (DQO), quantidade de fósforo, de nitrogênio, do glifosato, do nitrito e do nitrato, temperatura do reator, pH e Oxigênio Dissolvido. Os resultados demostraram que o tratamento do efluente contendo apenas o glifosato é mais eficiente do que o tratamento contendo o glifosato e o polímero hidroabsorvente.

Orientador: Alexandre Luiz Souto Borges / Coorientador: Artur José Monteiro Valente / Banca: Bruno Vinícius Manzolli Rodrigues / Banca: Fernanda Alves Feitosa / Banca: Lafayette Nogueira Júnior / Banca: Eduardo Shigueyuki Uemura / Resumo: Os atuais avanços no desenvolvimento de biomateriais caminham para 2 áreas promissoras: a de regeneração tecidual e a de entrega controlada de fármacos. Assim, o presente estudo objetivou a síntese e caracterização de diferentes matrizes (fibras e hidrogel) à base de quitosana, a fim de se obter materiais biomiméticos para atuação em ambas áreas. Para regeneração, delineou-se a síntese de um arcabouço de fibras de quitosana com e sem cristais de nanohidroxiapatita onde, para isso, foram eletrofiadas soluções de quitosana (Ch) e de quitosana com nanohidroxiapatita (ChHa). Os espécimes de Ch apresentaram maior homogeneidade e maior diâmetro médio de fibras (690 ± 102 nm) que ChHa (358 ± 49 nm). No teste de viabilidade celular e na atividade da fosfatase alcalina não houve diferença estatística entre os grupos experimentais (Ch e ChHa), porém ambos diferiram do grupo controle (p < 0,001). Para o âmbito de liberação de fármacos, sintetizou-se, pela técnica de emulsão, dois tipos de hidrogéis: o primeiro, uma mistura da fase aquosa da solução de Ch (1 mL) e da solução de DNA (1 mL) (1:1) e o segundo, mistura da fase aquosa da solução de Ch (1 mL) e solução de Pectina (1 mL) (1:1). Ambas misturas foram realizadas em álcool benzílico (5 mL) com instrumento de dispersão de alto desempenho (31-34000 rpm min-1 por 5 min). Após a obtenção dos géis, 20mg de cada grupo foram imersos em uma solução aquosa de Própolis Verde (PV), na concentração de 70 μg/mL por 2 h e a cinética de liberação... (Resumo completo, clicar acesso eletrônico abaixo) / Current advances in biomaterial development are moving to 2 promising areas: tissue regeneration and controlled drug delivery. Thus, the present study aimed the synthesis and characterization of different matrices (fibers and hydrogel) based on chitosan, in order to obtain biomimetic materials for performance in both areas. For regeneration, the synthesis of a scaffold of chitosan fibers with and without nanohydroxyapatite crystals was delineated, where chitosan (Ch) and chitosan with hydroxyapatite (ChHa) solutions were electrospun. Ch specimens presented higher homogeneity and larger mean fiber diameter (690±102nm) than ChHa (358 ± 49nm). In the cell viability test and alkaline phosphatase activity there was no statistical difference between the experimental groups. (Ch and ChHa), but both differed from the control group (p < 0,001). For the drug release scope, two types of hydrogels were synthesized by the emulsion technique: the first, a mixture of the aqueous phase of Ch solution (1 mL) and DNA solution (1 mL) (1:1) and the second, mixture of the aqueous phase of the Ch solution (1mL) and Pectin solution (1 mL) (1:1). Both mixtures were performed in benzyl alcohol (5 mL) with high performance dispersion instrument (31-34000 rpm min-1 for 5 min). After obtaining the gels, 20mg from each group were immersed in an aqueous solution of Propolis Green (PV), at a concentration of 70 µg/mL for 2 h and the release kinetics of PV were analyzed at 25 and 37oC in water and artificial saliva. The obtained specimens were lyophilized and then physically-chemically characterized. The presence of pectin and DNA was confirmed by FTIR. PV sorption induced a significant modification of the gel surface. A phase separation was found between chitosan and DNA. Encapsulation efficiency did not change significantly between 25 and 37oC. The release kinetics in water or saliva presented a two-step mechanism. And the biological results... / Doutor

京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第22037号 / 医博第4522号 / 新制||医||1038(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 髙橋 良輔, 教授 湊谷 謙司, 教授 井上 治久 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DGAM