Global ETD Search

1	The hydroperoxide moiety of aliphatic lipid hydroperoxides is not affected by hypochlorous acid Zschaler, Josefin, Arnhold, Jürgen 20 November 2015 (has links) (PDF) The oxidation of polyunsaturated fatty acids to the corresponding hydroperoxide by plant and animal lipoxygenases is an important step for the generation of bioactive lipid mediators. Thereby fatty acid hydroperoxide represent a common intermediate, also in human innate immune cells, like neutrophil granulocytes. In these cells a further key component is the heme protein myeloperoxidase producing HOCl as a reactive oxidant. On the basis of different investigation a reaction of the fatty acid hydroperoxide and hypochlorous acid (HOCl) could be assumed. Here, chromatographic and spectrometric analysis revealed that the hydroperoxide moiety of 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HpETE) and 13S-hydroperoxy-9Z,11E-octadecadienoic acid (13-HpODE) is not affected by HOCl. No reduction of the hydroperoxide group due to a reaction with HOCl could be measured. It could be demonstrated that the double bonds of the fatty acid hydroperoxides are the major target of HOCl, present either as reagent or formed by the myeloperoxidase-hydrogen peroxide-chloride system. Hypochlorsäure Fettsäurehydroperoxide Arachidonsäure Myeloperoxidase Lipoxygenase Hypochlorous acid Lipid hydroperoxides Arachidonic acid Myeloperoxidase Lipoxygenase ddc:610 ddc:612
2	Detection of the halogenating activity of heme peroxidases in leukocytes by aminophenyl fluorescein Flemmig, Jörg, Remmler, Johannes, Zschaler, Josefin, Arnhold, Jürgen 14 April 2016 (has links) (PDF) The formation of hypochlorous and hypobromous acids by heme peroxidases is a key property of certain immune cells. These products are not only involved in defense against pathogenic microorganisms and in regulation of inflammatory processes, but contribute also to tissue damage in certain pathologies. After a short introduction about experimental approaches for the assessment of the halogenating activity in vitro and in cell suspensions, we are focusing on novel applications of fluorescent dye systems to detect the formation of hypochlorous acid (HOCl) in leukocytes. Special attention is directed to properties and applications of the non-fluorescent dye aminophenyl fluorescein that is converted by HOCl, HOBr, and other strong oxidants to fluorescein. This dye allows the detection of the halogenating activity in samples containing free myeloperoxidase and eosinophil peroxidase as well as in intact granulocytes using fluorescence spectroscopy and flow cytometry, respectively. Myeloperoxidase Hypochlorsäure Neutrophile Aminophenyl fluorescein eosinophile Peroxidase hypobromige Säure Eosinophile myeloperoxidase hypochlorous acid neutrophils aminophenyl fluorescein eosinophil peroxidase hypobromous acid eosinophils ddc:570
3	The hydroperoxide moiety of aliphatic lipid hydroperoxides is not affected by hypochlorous acid Zschaler, Josefin, Arnhold, Jürgen 20 November 2015 (has links) The oxidation of polyunsaturated fatty acids to the corresponding hydroperoxide by plant and animal lipoxygenases is an important step for the generation of bioactive lipid mediators. Thereby fatty acid hydroperoxide represent a common intermediate, also in human innate immune cells, like neutrophil granulocytes. In these cells a further key component is the heme protein myeloperoxidase producing HOCl as a reactive oxidant. On the basis of different investigation a reaction of the fatty acid hydroperoxide and hypochlorous acid (HOCl) could be assumed. Here, chromatographic and spectrometric analysis revealed that the hydroperoxide moiety of 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HpETE) and 13S-hydroperoxy-9Z,11E-octadecadienoic acid (13-HpODE) is not affected by HOCl. No reduction of the hydroperoxide group due to a reaction with HOCl could be measured. It could be demonstrated that the double bonds of the fatty acid hydroperoxides are the major target of HOCl, present either as reagent or formed by the myeloperoxidase-hydrogen peroxide-chloride system. info:eu-repo/classification/ddc/610 ddc:610 info:eu-repo/classification/ddc/612 ddc:612
4	Detection of the halogenating activity of heme peroxidases in leukocytes by aminophenyl fluorescein Flemmig, Jörg, Remmler, Johannes, Zschaler, Josefin, Arnhold, Jürgen January 2015 (has links) The formation of hypochlorous and hypobromous acids by heme peroxidases is a key property of certain immune cells. These products are not only involved in defense against pathogenic microorganisms and in regulation of inflammatory processes, but contribute also to tissue damage in certain pathologies. After a short introduction about experimental approaches for the assessment of the halogenating activity in vitro and in cell suspensions, we are focusing on novel applications of fluorescent dye systems to detect the formation of hypochlorous acid (HOCl) in leukocytes. Special attention is directed to properties and applications of the non-fluorescent dye aminophenyl fluorescein that is converted by HOCl, HOBr, and other strong oxidants to fluorescein. This dye allows the detection of the halogenating activity in samples containing free myeloperoxidase and eosinophil peroxidase as well as in intact granulocytes using fluorescence spectroscopy and flow cytometry, respectively. info:eu-repo/classification/ddc/570 ddc:570

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