The L5 signal of the Global Positioning System (GPS) is becoming available on an increasing number of Block IIF satellites. As the third civilian signal, L5 is superior in signal design to the L1 C/A and L2C civilian signals. This new signal has been marked healthy for use on selected satellites since 2010, yet the hardware capable of tracking the L5 signal is still in the early stages of development. This work investigates the characteristics of the new signal and the quality of data produced by L5-tracking receivers. Commonly used receiver models chosen for this study are the Leica GRX1200+GNSS, the Trimble NetR8, and the Javad Delta TRE-G3TH. The metrics used in this analysis to assess the quality of data produced by these receivers are signal strength, receiver phase noise, receiver code noise, and multipath. The data used in these analyses were obtained from the International GNSS Service for the days of the year 275 to 281 in 2011. Metrics averaged over the GPS week 1656 provide a good indication of the overall performance of the receivers. / text

A protein, B0238.11, was identified in a yeast one-hybrid screen to bind to the ribosomal intergenic spacer region (IGS) of Caenorhabditis elegans. Proteins interacting with this region of the DNA have been implicated in ribosome biogenesis in other model organisms, so it is also possible that B0238.11 plays a role in RNA transcription by interacting with RNA polymerase I or other transcription machinery. Thus, the goal of this study was to further characterize the structure and function of B0238.11. I used yeast two-hybrid experiments to identify proteins that interact with B0238.11 within the nucleus. RPS-0, K04G2.2, DPY-4, EFT-3, PAL-1, and B0238.11, itself, were found to bind to B0238.11. Additionally, I analysed the amino acid sequence of B0238.11 using in silico bioinformatics methods to determine its structure and putative function and also to identify and characterize the other interacting proteins. I found that B0238.11 contains a high-mobility group box domain, which is also found in HMO1P in yeast and UBF in vertebrates. These other proteins also bind to the IGS, are known to form homodimers and have been implicated in the initiation of ribosomal RNA transcription. Here I scrutinize the validity of the interaction between each protein and B0238.11. I conclude that B0238.11 is likely to be a C. elegans homolog of UBF and present an updated interactome map for B0238.11. / Synopsis: I carried out yeast two-hybrid assay to find proteins interacting with B0238.11 (O16487_CAEEL). I found that this protein's DNA-binding profile and protein interaction profile mimic other HMG-box containing proteins UBF and HMO1P which are involved in ribosomal RNA transcription initiation. Acknowledgements: I would like to thank my supervisor, Dr. Teresa J. Crease, for not only giving me the opportunity to investigate an interesting topic in Molecular Biology, but also for her patient guidance, encouragement and sound advice. I feel extremely lucky to have a supervisor who cared so much about my work, who responded to my questions and queries so promptly, and was always available to discuss project and career related matters. I would also like to thank Dr. Todd Gillis and Dr. Terry Van Raay for their careful consideration of this project and timely constructive criticisms that helped shape my project. I would like to thank all the members of my committee for helping me see things from different perspectives and helping me develop and critical and mature understanding of the scientific process. I must also express my gratitude to Dr. Robin Floyd for allowing me to build upon his work and Dr. Marian Walhout, at the University of Massachusetts, for providing the Caenorhabditis elegans complimentary DNA library. A large part of this project would not have been possible without the people at the genomics facility in the Department of Integrative Biology, I commend their professionalism and punctuality in delivering results. Completing this work would have been all the more difficult were it not for the support and friendship provided by my peers Shannon Eagle, Tyler Elliott, Nick Jeffery, Joao Lima, Sabina Stanescu, Fatima Mitterboeck and Paola Pierossi. And finally, I would like to thank my parents and siblings Sara Omar and Ali Omar for their continued support through good times and bad, and letting me use their laptops when mine broke down.

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-15T16:52:35Z No. of bitstreams: 1 JACQUELINE LIEDJA ARAUJO SILVA CARVALHO - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1821177 bytes, checksum: 5edf83fb0f87d1e2e3a52d083f218e97 (MD5) / Made available in DSpace on 2018-05-15T16:52:35Z (GMT). No. of bitstreams: 1 JACQUELINE LIEDJA ARAUJO SILVA CARVALHO - DISSERTAÇÃO PPGSA PROFISSIONAL 2013..pdf: 1821177 bytes, checksum: 5edf83fb0f87d1e2e3a52d083f218e97 (MD5) Previous issue date: 2013-09-26 / O Brasil tem um dos maiores rebanhos bovino leiteiros do mundo. No entanto, apesar de estar entre os grandes produtores mundiais de leite, a pecuária leiteira nacional é bastante heterogênea. Parte de algumas regiões como o Nordeste brasileiro, ainda encontra-se em desenvolvimento para alcançar os padrões de qualidade e quantidade e chegar ao mercado competitivo, como por exemplo, o município de Pombal, que estar localizado no Sertão da Paraíba, área do presente estudo, que é reconhecida em âmbito estadual, por seu potencial leiteiro. Neste contexto, esta pesquisa tem o objetivo de fazer uma análise da sustentabilidade da bovinocultura leiteira de Pombal, nos seus aspectos institucional (tecnológica), social, econômica e ambiental. Para a execução desse estudo, foram necessárias visitas in loco e aplicação de questionários semi-estruturados, a 50 produtores de leite, pertencentes a trinta e duas áreas rurais desse município. A partir das respostas, foram calculados em indicadores, utilizando o método do PNUD/ONU (1998), cujos resultados variaram de 0 (zero) a 100 (cem) e foram divididos em quatro graus de sustentabilidades: crítico, ruim, bom e excelente. Em seguida, aplicado na fórmula matemática denominada de IGS (Índice Global de Sustentabilidade) desenvolvido por González e Carvajal (2002). Quando o resultado do IGS for menor ou igual a 50%, a atividade apresenta-se no campo da insustentabilidade e será mais sustentável próximo a 100%. Os resultados na dimensão institucional apresentaram índices de 37%, na dimensão social em 58%, ambos considerados graus ruins de sustentabilidade. Na dimensão ambiental índices de 60%, ambos considerados graus bons para a sustentabilidade e, finalmente, a dimensão econômica com grau 36%, em nível ruim para a sustentabilidade. O IGS calculado foi de 47,74% o que não pode ser considerado sustentável. Desta forma, os resultados obtidos identificam que a produção bovina leiteira, de Pombal, necessita de atenção, com mais políticas públicas para o setor, não apenas no aspecto econômico, mas também nas dimensões sociais e ambientais, possibilitando melhorias na qualidade de vida dos envolvidos, como também, na conservação dos recursos naturais. / Brazil has one of the largest dairy cattle herds in the world. However, despite being among the major world producers of milk, the national dairy industry is quite heterogeneous. Part of some regions such as the Brazilian Northeast, is still under development to meet the standards of quality and quantity and get the competitive market, such as the municipality of Pombal, to be located in the backlands of Paraiba, the present study area, which is recognized statewide for its dairy potential. In this context, this study aims to make an analysis of the sustainability of dairy cattle of Pombal, in their institutional (technological), social, economic and environmental aspects. For the execution of this study, site visits and application of semi-structured questionnaires, 50 dairy farmers, belonging to thirty- two rural areas of this municipality were necessary. From the responses, indicators were calculated using the method of UNDP / UN (1998), whose results ranged from 0 (zero) to 100 (one hundred) and were divided into four grades of sustainabilities: critical, poor , good or excellent. Then applied to the mathematical formula called GSI (Global Sustainability Index) developed by González and Carvajal (2002 ). When the result of the IGS is less than or equal to 50%, the activity is presented in the field of unsustainability will be more sustainable and close to 100% . The results presented in the institutional dimension ratios of 37%, the social dimension in 58%, both considered bad grades sustainability. In the environmental dimension ratios of 60%, both considered good grades for sustainability and ultimately the economic dimension with grade 36% in bad for the sustainability level . The calculated IGS was 47.74% which can not be considered sustainable. Thus, the results identify that the dairy cattle production, Pombal, needs attention, most public policies for the sector , not only in economics but also in social and environmental dimensions, enabling improvements in the quality of life of those involved, but also to conserve natural resources.