Thesis (doctoral)--Universität Trier, 2004. / Also available via the internet in PDF or HTML formats.

Thesis (Ph. D.)--Oregon State University, 2006. / Printout. Includes bibliographical references (leaves [88-98]). Also available on the World Wide Web.

Thesis (M.S.) University of Missouri-Columbia, 2006. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research .pdf file viewed on (June 25, 2007) Includes bibliographical references.

No description available.

Inhibiting the formation of ice is an essential process commercially, industrially, and medically. Compounds that work to stop the formation of ice have historically possessed drawbacks such as toxicity or prohibitively high active concentrations. One class of molecules, ice recrystallization inhibitors, work to reduce the damage caused by the combination of small ice crystals into larger ones. Recent advances made by the Ben lab have identified small molecule carbohydrate analogues that are highly active in the field of ice recrystallization and have potential in the cryopreservation of living tissue. A similar class of molecules, kinetic hydrate inhibitors, work to prevent the formation of another type of ice – gas hydrate. Gas hydrates are formed by the encapsulation of a molecule of a hydrocarbon inside a growing ice crystal. These compounds become problematic in high pressure and low temperature areas where methane is present - such as an oil pipeline. A recent study has highlighted the effects of antifreeze glycoprotein, a biological ice recrystallization inhibitor, in the inhibition of methane clathrates. Connecting these two fields through the synthesis and testing of small molecule ice recrystallization inhibitors in the inhibition of methane hydrates is unprecedented and may lead to a novel class of compounds.

The primary goal of this thesis is to utilize acoustical radiation from the Arctic ice cover to infer the response of sea ice to environmental forcing, and to sense remotely the mechanical properties of the ice. The work makes use of two experiments in the Canadian arctic undertaken by the Ocean Acoustics Group of the Institute of Ocean Sciences, which resulted in an extensive body of acoustical and related environmental data. Cracking sounds originating from both first and multi-year ice fracturing processes are analyzed. Data used in this thesis also include sound made by artificial sources. The survey of in situ ice conditions by air photography and synthetic radar imaging, and a crack distribution map based on observations made with a 3-D hydrophone array, reveal, for the first time, a close correlation between thermal cracking events and ice type. It is shown that most of the thermal cracks occur in irregular multi-year ice where there are exposed, snow-free surfaces. The study shows that acoustical radiation from some cracks implies a slip-stick seismic movement over the faults, and some cracks tend to radiate more high frequency sound downwards rather than sideways. This phenomenon is most clearly apparent in sounds made by artificial sources. Another interesting finding from this study is that the sound of cracking ice does not always exhibit a vertical dipole radiation pattern, and some cracks due to thermal tension on smooth first year ice radiate more energy horizontally. The observations have motivated the development of various analytical models. These models allow the observed acoustical features to be related to the length and depth of a crack, the thickness of the ice cover and its Young's modulus. The models also show that maximum sound radiation from a crack is in the direction of external forcing. Finally, it is found that noise due to rubbing between ice floes exhibits a narrow band spectrum. This phenomenon is investigated and a linear model derived shows that the observed peak frequency is that of the first mode horizontal shear wave triggered by frictional effects at the ice floe edge. / Science, Faculty of / Earth, Ocean and Atmospheric Sciences, Department of / Graduate

A study was made of the petrology of a variety of underground ice types in permafrost on the Tuktoyaktuk Peninsula and Pelly Island, Mackenzie Delta, N.W.T. Ice bodies of a considerable range of ages occur, including some deformed in the Wisconsin glaciation; also permafrost and ice is growing ab initio beneath recently drained lake bottoms. The spectrum of ice body size is also wide, extending from pore-sized particles to beds 25 m thick. The major objective of the study was an understanding of the growth and deformation of such ice bodies from a petrologic viewpoint. Thus several bodies of known, recent, age -were analyzed in order to enumerate features typical of growth. This was possible for icing mounds, tension cracks and active layer ice which grew in winter 1973-74. Growth conditions were inferred in terms of water supply, freezing directions and rates, solute rejection (bubble formation) and crystal size, shape, lattice and dimensional orientation. On the basis of this knowledge of growth features, older and larger ice bodies were studied, and post-solidification characteristics ware analyzed. Soma near-surface ice gave evidence of thermomigration of bubbles, but the major changes in fabric ware due to thermally and mechanically induced stresses. In the case of wedge ice, progressive changes in crystal size, shape, lattice and dimensional orientation ware recognized from the centre to the boundary of the wedge, due to recrystallization and grain growth associated with wedge development. Segregated ice was studied ia pingos and an involuted hill. A pingo core with steeply-dipping beds showed little evidence of flow while broader pingo with a greater pore ice content had undergone some flow in the segregated ice layers. A range of fabrics was found in the involuted hill, optic axis orientations becoming increasingly concentrated normal to compositional layering while dimensional orientations tended towards parallelism with the layering in anticlines in the ice. The influence of bubbles on deformation is pointed out in that larger crystals occur in clear ice and thus have greater intracrystalline slip than in bubbly ice. Where a wedge penetrated such a fold, the fabric changed along the fold limb in a manner symmetrically related to the wedge. Additionally, several near-surface ices ware studied and showed evidence of multiple growth periods, and multiple freezing directions, indicating that the ice grew in enclosed water in frozen material. Thus the complexity of freezing and melting histories may be recognized petro-graphically while it is not readily apparent in the field. / Arts, Faculty of / Geography, Department of / Graduate

The Greenland and Antarctic ice sheets are losing mass and contributing to global sea-level rise. Ice shelves, floating ice attached to the margins of the ice sheets, modulate sea-level rise by restraining ice-sheet flow out towards the oceans, but are sensitive to surface melting. The formation of surface meltwater lakes on ice shelves can trigger rapid ice-shelf collapse. However, surface meltwater also flows atop ice shelves through rivers. The impact of rivers on ice-shelf stability is unknown. Previous studies of ice-shelf hydrology hypothesize that rivers mitigate the damage-potential of lakes by removing surface water off of the ice shelf, but also suggest that rivers enhance ice-shelf fracturing by incising into areas of already thin ice. This dissertation is focused on exploring the role of rivers on ice-shelf stability using remote sensing datasets, conceptual models, and Augmented Reality (AR). Focusing on ice shelves in Greenland, I present the discovery of a new ice-shelf surface hydrology feature, an ice-shelf estuary, and demonstrate its potential to weaken ice shelves. I fully document this new process on the Petermann Ice Shelf, where flow reverses at the mouth of the Petermann Estuary. This study marks the first observation of ocean water atop an ice shelf. I also document the initiation and growth of fracturing along the estuary channel, and a history of rectilinear calving events, where icebergs calve along longitudinal rivers. Based on this analysis of the Petermann Estuary, I propose a new mechanism for damaging ice shelves: estuarine weakening. I present evidence that this process also occurs on the Ryder Ice Shelf in northwest Greenland. My analysis demonstrates that the role of rivers on ice-shelf stability depends on how the river mouth evolves. If ice-shelf waterfalls at the river mouth incise to sea level and form estuaries, flow reversal will modulate water export off the shelf and maintain the damage-potential of lakes, and estuarine weakening may lead to a new mode of ice-shelf calving. By analyzing the three-dimensional (3D) structure of the Petermann and Ryder Ice Shelves and Estuaries with remote sensing and radar data, I find that basal channels are an important driver of estuary development as they dictate the linearity of surface rivers. Determining the role that basal channels play in estuary formation requires accurate and appropriate data visualization tools. I develop AR applications to visualize radar data on ice shelves, towards enabling more intuitive and sophisticated interpretation of the ice-shelf structure in 3D. Through simple conceptual modeling, I suggest that although basal channels precondition ice-shelf estuary formation, estuary formation is strongly controlled by river incision. Finally, I present a model of ice-shelf estuary formation as a function of surface and basal melting. Using this conceptual model, I predict that ice-shelf estuaries could form in Antarctica in the near future. Surface melting in Antarctica is predicted to increase in under half a century. Estuary formation in Antarctica will be accelerated by lengthening of the melt season, and estuaries may form far from the calving front if rivers intersect upstream rifts. I show that ice-shelf estuaries could evolve from ice-shelf rivers in a warming Antarctica, introducing new ice-shelf weakening mechanisms. This increases the urgency to understand and include ice-shelf estuarine processes in ice-sheet models.

Les mycoplasmes sont des bactéries à petit génome dérivées d’ancêtres à Gram positif par une succession de pertes de matériel génétique. Il a longtemps été considéré que la réduction génétique était la seule force régissant l’évolution de ces bactéries, cependant, des transferts horizontaux de grandes régions chromosomiques au sein et entre les espèces de mycoplasmes ont été récemment mis en évidence. Des éléments conjugatifs et intégratifs (ICE) découverts chez certaines espèces de mycoplasme pourraient être à l’origine de ces transferts. Ces ICEs codent les systèmes nécessaires pour leur excision, leur transfert conjugatif et leur intégration dans la cellule receveuse.Mycoplasma hominis est un mycoplasme commensal des voies génitales qui peut être responsable d’infections gynécologiques, d’infections néonatales et d’infections extragénitales. L’analyse préliminaire de génomes de M. hominis avait montré la présence de régions codantes caractéristiques des ICEs. Les objectifs de cette thèse étaient de rechercher et caractériser les ICEs chez 12 isolats cliniques de M. hominis entièrement séquencés et de déterminer la prévalence de ces ICEs au sein de l’espèce M. hominis. Pour cela, une étude rétrospective sur une période de 6 ans a été menée sur des isolats cliniques obtenus au CHU de Bordeaux. Les concentrations minimales inhibitrices des tétracyclines et des fluoroquinolones ainsi que les mécanismes de résistance ont été déterminés, permettant de disposer d’une collection d’isolats cliniques caractérisés pour l’étude des ICEs.Des ICEs de près de 30 kpb ont été trouvés en une ou plusieurs copies dans sept des 12 souches de M. hominis séquencées. Seulement cinq de ces ICEs semblaient fonctionnels puisqu’une forme circulaire a pu être détectée. Tous les ICEs de M. hominis présentaient une structure similaire avec un module spécifique de M. hominis d’environ 4-kpb, codant des protéines ayant des caractéristiques structurelles similaires à des effecteurs TAL (transcription activator-like), impliqués dans la reconnaissance de nucléotides et dans la transduction de signaux chez les bactéries symbiotiques. La caractérisation des mécanismes de résistance aux antibiotiques des isolats cliniques de M. hominis collectés au CHU de Bordeaux nous a permis de disposer d’une collection de 183 isolats isolés entre 2010 et 2015, parmi lesquels 14,8% étaient porteur du gène tet(M) responsable de la résistance aux tétracyclines, 2,7% étaient résistant à la lévofloxacine et 1,6% étaient résistants à la moxifloxacine par mutation des gènes de la topoisomérase IV et de l’ADN gyrase. Le screening de 120 de ces isolats cliniques a révélé une prévalence élevée des ICEs dans l’espèce M. hominis, mesurée à 45%. Il n’y avait pas de prédominance des ICEs dans les isolats portant le gène tet(M), suggérant que les ICEs n’étaient pas responsables de la dissémination de la résistance à la tétracycline.Des expériences complémentaires de conjugaison seront nécessaires pour confirmer la fonctionnalité des ICEs retrouvés dans l’espèce M. hominis. Cependant, la forte prévalence et le caractère très conservé des ICEs chez M. hominis suggèrent que ces ICEs pourraient conférer un avantage sélectif pour la physiologie ou la physiopathologie de la bactérie. Ce travail ouvre ainsi la voie à de futures études qui permettront une meilleure compréhension des transferts horizontaux de gènes et des facteurs de virulence chez M. hominis. / Mycoplasmas are small-genome bacteria derived from Gram-positive ancestors by a succession of genetic material losses. It has long been considered that genetic reduction was the only force governing the evolution of these bacteria, however, horizontal transfers of large chromosomal regions within and between mycoplasma species have recently been reported. Conjugative and integrative elements (ICE) found in some species of mycoplasma may be responsible for these transfers. These ICEs encode the systems necessary for excision, conjugative transfer and integration into a recipient cell.Mycoplasma hominis is a commensal genital mycoplasma that can be responsible for gynecological infections, neonatal infections and extragenital infections. Preliminary analysis of M. hominis genomes had showed the presence of coding regions characteristic of ICEs. The objectives of this thesis were to search for and characterize ICEs in one reference strain and 11 fully sequenced M. hominis clinical isolates and to determine the prevalence of these ICEs in the M. hominis species. To do so, a retrospective study over a period of 6 years was conducted on clinical isolates collected at the Bordeaux University Hospital. The minimum inhibitory concentrations of tetracyclines and fluoroquinolones as well as resistance mechanisms were determined, providing a collection of clinical isolates characterized for the study of ICEs.ICEs of 27-30 kpb were found in one or two copies in seven of the 12 M. hominis sequenced strains. Only five of these ICEs seemed functional since circular forms of extrachromosomal ICE were detected. All M. hominis ICEs exhibited a similar structure consisting of a 4.0-5.1 kb module composed of five to six juxtaposed CDSs, encoding proteins that share common structural features with transcription activator-like (TAL) effectors, involved in polynucleotide recognition and signal transduction in symbiotic bacteria. The characterization of antibiotic resistance mechanisms in M. hominis clinical isolates collected at Bordeaux University Hospital enabled us to obtain a collection of 183 isolates isolated between 2010 and 2015, of which 14.8% harbored the tet(M) gene responsible for tetracycline resistance, 2.7% were resistant to levofloxacin and 1.6% were resistant to moxifloxacin by mutation in topoisomerase IV and DNA gyrase genes. Screening of 120 of these clinical isolates revealed a high prevalence of ICEs in M. hominis, measured to be 45%. The proportion of ICEs was not higher in isolates carrying the tet (M) gene, suggesting that ICEs were not responsible for the spread of tetracycline resistance.Additional mating experiments will be necessary to confirm the functionality of the ICEs found in the M. hominis species. However, the conserved and specific structure of M. hominis ICEs and the high prevalence in clinical strains suggest that these ICEs may confer a selective advantage for the physiology or pathogenicity of the bacteria. This work opens the way for future studies that will provide a better understanding of horizontal gene transfers and virulence factors in M. hominis.

Thesis (M.A.)--Boston University / Introduction The purpose of the thesis is; to develop and explain the location and extent of sea ice in the Beaufort Sea, and to describe methods in use and proposed by the science of ice forecasting to aid ship movements in the area. Much of the data in the thesis camas from unpublished or widely scattered papers, and the thesis is an attempt to assimilate such data into cohesive form. Chapter I. The Newly Acquired Importance of the Beaufort Sea Region and the Status of Ice Forecasting. Prior to World War II the area of the Beaufort Sea was little known or exploited. A few explorers occasionally had joined the native eskimos as inhabitants of the area. After the war, a program of oil exploration was undertaken on the southern shores of the Sea by the U.S. Navy. Discovery of oil and the newly feasible means of making trans-polar flights across the Arctic waters suddenly catipulated the area into a position of strategic importance to our national defense. Research programs devoted to gathering data on how to live in such a hostile climate bacame a natural outgrowth of this new importance. With the construction and resupply of the DEW line a vital necessity, methods of coping with sea ice had to be developed. In this respect, the Russians were far advanced over the Western Allies, having devoted much time and effort on the problem since the 1920's. Nonetheless, U.S. and Canadian scientists, under an accelerating research program, are well on the way to equality in the fields surrounding sea ice research. [TRUNCATED]