Geological records of past sea ice, such as those contained in Arctic marine sediments, offer an opportunity to strengthen our understanding of long-term sea ice variability, provided unambiguous paleo-sea ice proxies can be developed. One such recently proposed proxy is IP₂₅, a highly branched isoprenoid alkene biosynthesized exclusively by sea-ice dwelling diatoms (Haslea spp.), which is well preserved in marine sediments and could be used to reconstruct past changes in spring sea-ice extent. However, little is known about regional-scale controls on IP₂₅ production in sea ice, limiting its wider applicability as a paleo-sea-ice proxy. To address this issue we examined the distributions of IP₂₅ and the marine productivity biomarkers dinosterol and brassicasterol in a suite of surface sediment samples distributed across the Arctic. We find a statistically significant, logarithmic relationship between IP₂₅ and spring sea ice cover in samples from arctic and subarctic sites in the Pacific (n = 96, r² = 0.67, P < 0.0001) and the Atlantic n = 25, r² = 0.50, P < 0.0001), though the absolute concentrations of IP₂₅ are generally higher in the Atlantic (1.6 - 166.4 [mu]g/g OC) than in the Pacific (0 - 38.5 [mu]g/g OC) for equivalent sea-ice cover, and there are regional and basin-specific differences in the slope of the IP₂₅ - sea ice relationship. After normalization of IP₂₅ concentrations to that of a productivity biomarker (e.g., dinosterol; the PDIP₂₅ index) the proxy-sea ice relationship in greatly improved for all regions (r² = 0.86 and r² = 0.75 for Atlantic and Pacific, respectively) and most of the basin specific differences in the rate of change of IP₂₅ with sea ice are removed. This suggests that productivity plays an important secondary role in controlling IP₂₅ concentrations. However, the use of the PDIP₂₅ index does not change the absolute differences in concentrations seen in the Atlantic and the Pacific, and previously published data from Fram Strait remain anomalous when compared to the rest of our data. This suggests that there are additional, yet unidentified controls on the IP₂₅ proxy - sea ice relationship, which may hinder the development of an Arctic-wide calibration but that the PDIP₂₅ index is a viable tool for local and regional sea ice reconstructions. / text

Large ice blocks containing enough sediment to be denser than sea water form in the Minas Basin of the Bay of Fundy. The timing of ice block formation and ice block composition were monitored to improve understanding of the potential threat to tidal power generators posed by collision with ice blocks. Large blocks are produced from ice cliffs that form when anchored ice obstructs tidal channels and decreases flow speed. Decreased flow causes the channel cross-sectional area to decrease. In 2012, the Kennetcook River cross- sectional area decreased by 21% due to the formation of ice cliffs. Large ice blocks separated from the walls during the two spring tides following a 20-day time lag of the minimum air temperature. Ten percent of sampled ice blocks were denser than freshwater. Four of twelve ice cores collected from the ice cliffs along the Kennetcook River contained enough sediment to become denser than seawater.

The objective of this study was to investigate the potential application of rice bran wax (RBW) organogel to replace solid fat content and create the fat network in ice cream. Ice creams with 10% fat or 15% fat were formulated with RBW organogel as the fat source, and two different emulsifiers were used: polmo, a commercial blend of emulsifiers which contains 80% mono- and diglycerides and 20% polysorbate 80, and Glycerol monooleate (GMO). Candelilla wax (CDW) organogel and carnauba wax (CBW) organogel were also tested for comparison. RBW organogel had the ability to form and sustain structure in 15% fat ice creams when GMO was used as the emulsifier. Transmission electron microscopy revealed that the RBW crystal morphology within the fat droplet, when GMO was used as the emulsifier, was characterized by the growth of crystals at the outer edge of the droplet which increased fat destabilization and network formation. / Nestlé Product Technology Centre, France

Antifreeze proteins (AFPs) help cold-adapted organisms survive below 0 ◦C by binding to and inhibiting the growth of ice crystals. In this way, AFPs depress the freezing point of aqueous fluids below the melting point of ice (thermal hysteresis; TH). They also have the ability to inhibit ice recrystallization in the frozen state (ice recrystallization inhibition; IRI). Some AFPs show an order of magnitude higher TH activity than others, and are termed ‘hyperactive’. One of the objectives of this thesis was to see if IRI activities of the hyperactive AFPs are also an order of magnitude higher than the moderately active AFPs. Using a capillary-based assay for IRI, the activities of three hyperactive and three moderately active AFPs were determined. There was no apparent correlation between hyperactivity in TH and high IRI activity. However, mutations of residues on the ice-binding face (IBF) of both types of AFP reduced IRI and TH activities to a similar extent. In this way, the use of IBF mutant AFPs showed that the IBF responsible for an AFP’s TH activity is also responsible for its IRI activity. Analysis of the diverse AFP structures solved to date indicate that their IBFs are relatively flat, occupy a significant proportion of the protein’s surface area and are more hydrophobic than other surfaces of the protein. The IBFs also often have repeating sequence motifs and tend to be rich in alanine and/or, threonine. The de novo design of an ice-binding protein was undertaken using these features to verify the underlying physicochemical requirements necessary for a protein’s interaction with ice. Using site-directed mutagenesis, a total of sixteen threonine substitutions were made on one of the four faces of a cyanobacterial protein with no endogenous TH activity. The inclusion of eight paired threonines on one face of this quadrilateral helix gave the engineered protein low levels of TH activity, but at the cost of destabilizing the structure to some extent. The results of this study have validated some of the properties needed for the ice-binding activity of AFPs. / Thesis (Master, Biochemistry) -- Queen's University, 2010-01-29 17:37:24.322

Microbes have a remarkable ability to adapt to a host of environmental stressors, including low temperature, high pressure and osmotic stresses. The adaptations of resistant microbes to low temperatures are varied, and may include the accumulation of solutes to maintain osmotic balance, the production of antifreeze proteins (AFPs) or ice nucleation proteins (INPs) to manipulate ice growth or formation. AFPs depress the freezing point, inhibit ice recrystallization, and have been reported to inhibit or delay the growth of gas hydrates. Conversely, INPs precipitate ice formation at relatively high subzero temperatures. Collectively, these activities can be described as ‘ice-association’ activities. Here, ice-affinity and/or freeze-thaw cycling were used to either select for isolates with ice association properties or to assess the low temperature resistance of microbial consortia derived from various environments. Ice-affinity successfully selected psychrotolerant microbes from cultured temperate and boreal soils, some of which had been previously reported in glaciers and Arctic/Antarctic sites. Many of the recovered microbes demonstrated ice-association activities. Freeze-thaw selection also greatly decreased the abundance and diversity of consortia from distinct sites, and allowed the recovery of individual isolates, many of which demonstrated ice-association. Freeze-thaw selection was also used to assess the role of cross-tolerance between osmotic and freeze-thaw stresses, based on the common challenge of desiccation. Microbial consortia from lakes with varying degrees of salinity were subjected to freeze-thaw stress, and the consortia from more saline lakes tended to show greater low temperature resistance. While few of the recovered microbes demonstrated ice-association activities, those from the more saline lakes tended to contain a higher intracellular solute concentration and were more likely to form biofilms. This underscores the diversity of resistance strategies and supports the notion of cross-tolerance. To determine if these selective regimes would have applications for hydrate growth inhibition, microbes derived from an oil well sample were subjected to freeze-thaw stress. Selection reduced microbial abundance, shifted the diversity, and resulted in the recovery of microbes with some ice-association activity. Taken together, this thesis demonstrates that the application of low temperature stress can be used to successfully investigate stress resistance mechanisms within microbial communities from distinct environments. / Thesis (Ph.D, Biology) -- Queen's University, 2010-09-21 15:58:14.932

No description available.

No description available.

Snow and lake ice have very high albedos compared to other surfaces found in nature. Surface albedo is an important component of the surface energy budget especially when albedos are high since albedo governs how much shortwave radiation is absorbed or reflected at a surface. In particular, snow and lake ice albedos have been shown to affect the timing of lake ice break-up. Lakes are found throughout the Northern Hemisphere and lake ice has been shown to be sensitive to climatic variability. Therefore, the modelling of lake ice phenology, using lake ice models such as the Canadian Lake Ice Model (CLIMo), is important to the study of climatic variability in the Arctic and sub-Arctic regions and accurate snow and lake ice albedo measurements are required to ensure the accuracy of the simulations. However, snow and lake ice albedo can vary from day-to-day depending on factors such as air temperature, presence of impurities, age, and composition. Some factors are more difficult than others to model (e.g. presence of impurities). It would be more straight forward to just gather field measurements, but such measurements would be costly and lakes can be in remote locations and difficult to access. Instead, CLIMo contains an albedo parameterization scheme that models the evolution of snow and lake ice albedo in its simulations. However, parts of the albedo parameterization are based on sea-ice observations (which inherently have higher albedos due to brine inclusions) and the albedo parameterization does not take ice type (e.g. clear ice or snow ice) into account. Satellite remote sensing via the Moderate Resolution Imaging Spectroradiometer (MODIS) provides methods for retrieving albedo that may help enhance CLIMo’s albedo parameterization. CLIMo’s albedo parameterization as well the MODIS daily albedo products (MOD10A1 and MYD10A1) and 16-day product (MCD43A3) were evaluated against in situ albedo observations made over Malcolm Ramsay Lake near Churchill, Manitoba, during the winter of 2012. It was found that the snow albedo parameterization of CLIMo performs well when compared to average in situ observations, but the bare ice parameterization overestimated bare ice albedo observations. The MODIS albedo products compared well when evaluated against the in situ albedo observations and were able to capture changes in albedo throughout the study period. The MODIS albedo products were also compared against CLIMo’s melting ice parameterization, because the equipment had to be removed from the lake to prevent it from falling into the water during the melt season. Cloud cover interfered with the MODIS observations, but the comparison suggests that MODIS albedo products retrieved higher albedo values than the melting ice parameterization of CLIMo. The MODIS albedo products were then integrated directly into CLIMo in substitution of the albedo parameterization to see if they could enhance break-up date (ice off) simulations. MODIS albedo retrievals (MOD10A1, MYD10A1, and MCD43A3) were collected over Back Bay, Great Slave Lake (GSL) near Yellowknife, Northwest Territories, from 2000-2011. CLIMo was then run with and without the MODIS albedos integrated and compared against MODIS observed break-up dates. Simulations were also run under three difference snow cover scenarios (0%, 68%, and 100% snow cover). It was found that CLIMo without MODIS albedos performed better with the 0% snow cover scenario than with the MODIS albedos integrated in. Both simulations (with and without MODIS albedos) performed well with the snow cover scenarios. The MODIS albedo products slightly improved CLIMo break-up simulations when integrated up to a month in advance of actual lake ice break-up for Back Bay. With the MODIS albedo products integrated into CLIMo, break-up dates were simulated within 3-4 days of MODIS observed break-up. CLIMo without the MODIS albedos still performed very well simulating break-up within 4-5 days of MODIS observed break-up. It is uncertain whether this was a significant improvement or not with such a small study period and with the investigation being conducted at a single site (Back Bay). However, it has been found that CLIMo performs well with the original albedo parameterization and that MODIS albedos could potentially complement lake-wide break-up simulations in future studies.

Inhibiting the formation of ice is an essential process commercially, industrially, and medically. Compounds that work to stop the formation of ice have historically possessed drawbacks such as toxicity or prohibitively high active concentrations. One class of molecules, ice recrystallization inhibitors, work to reduce the damage caused by the combination of small ice crystals into larger ones. Recent advances made by the Ben lab have identified small molecule carbohydrate analogues that are highly active in the field of ice recrystallization and have potential in the cryopreservation of living tissue. A similar class of molecules, kinetic hydrate inhibitors, work to prevent the formation of another type of ice – gas hydrate. Gas hydrates are formed by the encapsulation of a molecule of a hydrocarbon inside a growing ice crystal. These compounds become problematic in high pressure and low temperature areas where methane is present - such as an oil pipeline. A recent study has highlighted the effects of antifreeze glycoprotein, a biological ice recrystallization inhibitor, in the inhibition of methane clathrates. Connecting these two fields through the synthesis and testing of small molecule ice recrystallization inhibitors in the inhibition of methane hydrates is unprecedented and may lead to a novel class of compounds.

No description available.