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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Tecnologias aplicadas à identificação do papel dos lipídios durante a organogênese em mamíferos (modelos suíno e canino) / Technologies applied to the identification of the role of lipids during organogenesis in mammals (swine and canine models)

Cabrera, Marisol León 19 December 2018 (has links)
O desenvolvimento embrionário encontra-se dividido em três etapas nas quais podem ser distinguidas as três estruturas: ovo, embrião e feto. Durante o desenvolvimento ocorrem eventos distintos e alterações morfológicas que dão inicio à vida. Os lipídios desempenham um papel importante no processo de formação, pois tem uma participação específica no desenvolvimento embrionário. A utilização de um conjunto de técnicas poderia auxiliar e decrever melhor os processos de formação e a sinalização molecular do desenvolvimento. Porém, o objetivo proposto neste trabalho foi caracterizar de forma aprimorada (mediante tecnologias apropriadas) o desenvolvimento embrionário de mamíferos, em relação à identificação de grupos lipídios. Para isso, foram estudados 28 fetos e embriões de cães, com idades compreendidas entre os 22 e 55 dias de gestação, obtidos em campanha de castração, além de um feto de suíno de 50 dias de gestação, procedente de um fornecedor comercial (Animal Technologies, Inc.). Foram analisados fígado, coração, cérebro, rins e pulmões de embriões e fetos, valendo-se de tecnologias de imagem (Microscopia Eletrônica de Varredura, Tomografia Computadorizada, Raios-X), e técnicas histológicas (colorações de Hematoxilina e Eosina, Tricrômio de Masson, Ferro Coloidal, Sudan Black) em cães. No suíno realizou-se além das citadas técnicas, a análise lipidômica relizada pela técnica de Electroespraiamento por Dispersão- Imageamento por Espectrometria (DESI-MS Imagem) e, posteriormente, coloração com Hematoxilina e Eosina. As principais conclusões relacionam-se ao emprego de várias tecnologias para descrever o desenvolvimento embrionário e fetal, caracterizando bem o desenvolvimento de estruturas, tais como: os arcos braquiais e as vesículas ópticas e óticas nos embriões, cordas tendíneas no coração, lobos hepáticos e mineralização do tecido ósseo. A vinculação dos lipídios à essas tecnologias permitiu associá-las aos processos de contração miocárdica, formação de surfactante pulmonar e trânsito de metabólitos das células epiteliais. Além disso, permitiram ainda demostrar as distribuições lipídicas em órgãos: Nacetilglutamina no coração, ST (ht22: 0) nos intestinos, AG dímeros no fígado e um amplo número de lipídios PS no sistema nervoso. Conclui-se que, de modo geral, existe grande variabilidade na interação do papel dos lipídios com o desenvolvimento embrionário. / Embryonic development is divided into three stages in which the three structures can be distinguished; egg, embryo and fetus. During development occur distinct events, and morphological changes that start life. Lipids play an important role in the formation process because they have a specific participation in embryonic development. The use of a set of techniques could help and improve the processes of formation and the molecular sign of embryonic development. However, the objective of this work was to characterize the embryonic development of mammals in an improved way (through appropriate technologies), in relation to the identification of lipid groups. For this, 28 fetuses and embryos of dogs, aged between 22 and 55 days of gestation, obtained in a castration campaign, in addition to a fetus of 50 days\' gestation from a commercial supplier (Animal Technologies, Inc.). The liver, heart, brain, kidneys and lungs of embryos and fetuses were analyzed using imaging technologies (Scanning Electron Microscopy, Computed Tomography, X-rays) and histological techniques (Hematoxylin and Eosin stains, Masson\'s Tricomial , Colloidal Iron, Sudan Black) in dogs. In the pig, all the techniques were performed, the lipid analysis revealed by the technique of Dispersion Electrospray - Spectrometry Imaging (DESI-MS Image) and subsequently staining with Hematoxylin and Eosin. The main conclusions are related to the use of several technologies to describe embryonic and fetal development, characterizing well the development of structures such as brachial arches and optic and optical vesicles in embryos, chordae tendineae in the heart, hepatic lobes and tissue mineralization bone. The lipid binding to these technologies has been associated with the processes of myocardial contraction, formation of pulmonary surfactant and transit of epithelial cell metabolites. In addition, they allowed to demonstrate the lipid distributions in organs: specific distributions were shown in organs: N-acetylglutamine in the heart, ST (ht22: 0) in the intestines, AG dimers in the liver, and a large number of PS lipids in the nervous system. We conclude that in general there is great variability in the interaction of the role of lipids with embryonic development.
2

Technological innovation and industrial evolution : the emergence of industrial networks

Lundgren, Anders January 1991 (has links)
Technological innovation is one of the most important sources of corporate and industrial growth. Yet, we know notably little of the relationship between innovation and economic change. Knowledge about the interaction between technological innovation and social action is suggested to be essential in the formulation of public and business policies concerning technological development. In this dissertation technological innovation is set into the context of industrial networks, here perceived as technological systems and networks of exchange relationships. The focus is upon the emergence of a technological system, digital image technology, and evolution of new patterns of interaction between actors, research organizations, established firms, new ventures and public institutions developing, producing, applying and promoting the new technology. The study of the development of digital image technology in Sweden between 1965 and 1989 shows that the emergence and the evolution of new industrial networks can be treated as three parallel processes; genesis – identification, coalescence – legitimation and dissemination – adaption, each dominating in different periods of time. Quantitative network analysis of the emerging patterns of interaction is used to analyze the changing structure of the Swedish image processing network resulting from these three evolutionary processes. The analysis reinforces the proposed framework for the understanding of the emergence of an industrial network.
3

Brinca_Comigo: os sujeitos imagéticos e suas sensibilidades projetadas / Brinca_Comigo: the imagery subjects and their projected sensitivities

Cirino, Renato 29 July 2013 (has links)
Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-11-03T17:34:29Z No. of bitstreams: 2 Dissertação - Renato Cirino Machado Alves Pereira - 2013.pdf: 4137112 bytes, checksum: f1993dcc574a6470a5bfb61f4e06ff96 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2014-11-03T17:35:31Z (GMT) No. of bitstreams: 2 Dissertação - Renato Cirino Machado Alves Pereira - 2013.pdf: 4137112 bytes, checksum: f1993dcc574a6470a5bfb61f4e06ff96 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-11-03T17:35:31Z (GMT). No. of bitstreams: 2 Dissertação - Renato Cirino Machado Alves Pereira - 2013.pdf: 4137112 bytes, checksum: f1993dcc574a6470a5bfb61f4e06ff96 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-07-29 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / The present work is a result of a reflection about an esthetic experience provided by the creation and execution of an interactive art installation in a formal education context. In the scope of the research, the installation Brinca Comigo (Play with me) is the tool-stimulus used to discuss how affection and emotional manifestations, mediated by sensibility and imaginary, can be projected by technical images in the process of socialization between high school students. The objective of the game, through the concept of game in Vilém Flusser’s perspective, triggers reflections about possible roles that digital images can assume in the established relations between researched imagistic subjects and the use of technological apparatus of the images and the information of the education process in a public educational institution of State of Goiás. / O presente trabalho é fruto da reflexão sobre a experiência estética proporcionada pela criação e execução de uma instalação de arte interativa no contexto da educação formal. No âmbito da pesquisa, a instalação Brinca_Comigo é a ferramenta-estímulo utilizada para discutir como manifestações de afetos e emoções, mediadas pelas sensibilidades e pelo imaginário, podem ser projetadas por imagens técnicas no processo de socialização entre estudantes do ensino médio. A brincadeira tem como objetivo, por meio do jogo na perspectiva abordada por Vilém Flusser, deflagrar reflexões sobre possíveis papeis que as imagens digitais possam exercer nas relações estabelecidas entre os sujeitos imagéticos pesquisados e o uso de aparatos tecnológicos da imagem e da informação no processo de vivência escolar em uma instituição pública pertencente à rede pública de ensino do Estado de Goiás.

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