No / In the past two decades comparative genomic hybridization (CGH) and array CGH have become crucial and indispensable tools in clinical diagnostics. Initially developed for the genome-wide screening of chromosomal imbalances in tumor cells, CGH as well as array CGH have also been employed in genotoxicology and most recently in toxicogenomics. The latter methodology allows a multi-endpoint analysis of how genes and proteins react to toxic agents revealing molecular mechanisms of toxicology. This chapter provides a background on the use of CGH and array CGH in the context of genotoxicology as well as a protocol for conventional CGH to understand the basic principles of CGH. Array CGH is still cost intensive and requires suitable analytical algorithms but might become the dominating assay in the future when more companies provide a large variety of different commercial DNA arrays/chips leading to lower costs for array CGH equipment as well as consumables such as DNA chips. As the amount of data generated with microarrays exponentially grows, the demand for powerful adaptive algorithms for analysis, competent databases, as well as a sound regulatory framework will also increase. Nevertheless, chromosomal and array CGH are being demonstrated to be effective tools for investigating copy number changes/variations in the whole genome, DNA expression patterns, as well as loss of heterozygosity after a genotoxic impact. This will lead to new insights into affected genes and the underlying structures of regulatory and signaling pathways in genotoxicology and could conclusively identify yet unknown harmful toxicants.

No description available.

Thesis ((DMed)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: We have developed an innovative multimedia e-learning resource, the Electrolyte Workshop, to provide students and clinicians with instruction and the opportunity for simulated practice in managing electrolyte and acid-base disorders. Our teaching approach is built around relevant physiology and makes use of real cases and storytelling to engage the learner. We have documented the challenges encountered during the development process and have made recommendations for the managing of similar projects. While there are many factors that must be in place to ensure successful e-learning, this dissertation focuses on an important but under-appreciated factor, namely the usability of the computer interface. Usability describes how easy technology interfaces are to use and is routinely evaluated and optimized in the software development industry. This is not yet the case with e-learning, especially in the area of medical education. Poor usability limits the potential benefit of educational resources, as this means that learners will struggle with the interface as well as with the challenges of the content presented. A comprehensive usability evaluation of our Electrolyte Workshop was completed. This included testing with typical end-users, where data were collected via standardized questionnaires and by observing and analysing their interactions with our application. We employed heuristic evaluation as an additional approach and assembled a panel of experts to evaluate our application against a set of heuristics, or principles of good interface design. Many serious usability problems were identified, thus severely limiting the potential educational impact of our Electrolyte Workshop. There was a striking disconnect between the objective measures of usability and self-reported questionnaire data. Our user-testing data make a useful contribution to the debate on how many users are required to find most of the usability problems in an interface. Heuristic evaluation proved to be a very efficient approach. However, both user testing and heuristic evaluation detected serious problems which were missed with the other method. These evaluations informed a comprehensive revision of our application and we could then compare the original with an optimized version in a randomized trial. We found large improvements in objective usability measures, which are likely to increase the satisfaction and motivation of learners. There were similar scores on measures of learning. This was not surprising as our participants were all relatively high-knowledge learners and not novices as regards the subject matter. Our study clearly indicates that the usability evaluation of e-learning resources is critical, and provides an example of how clinician-teachers can improve the usability of the resources they develop. Usability should be evaluated as a routine part of the development and implementation of e-learning materials, modules and programmes. This should start with the earliest versions of the resource, when making changes is easier and less costly. We have demonstrated that a combination of methods should be employed and have highlighted the utility of heuristic evaluation. An iterative approach should be followed, with several cycles of testing and re-design. User testing should always include the study of objective usability measures and not rely only on self-reported measures of user satisfaction. / AFRIKAANSE OPSOMMING: Ons het ’n innoverende multimediahulpbron vir e-leer, die Electrolyte Workshop, ontwikkel om studente en klinici van ’n onderrighulpmiddel sowel as die geleentheid vir gesimuleerde oefening in die hantering van elektroliet en suur-basis stoornisse te voorsien. Ons onderrigbenadering is gegrond op relevante fisiologie en maak gebruik van werklike gevalle en vertelkuns om die leerder te betrek en te boei. Ons het die uitdagings gedurende die ontwikkelingsproses opgeteken en aanbevelings oor die bestuur van soortgelyke projekte gedoen. Hoewel suksesvolle e-leer van etlike faktore afhang, konsentreer hierdie verhandeling op ’n belangrike dog onderskatte faktor, naamlik die bruikbaarheid van die rekenaarkoppelvlak. Bruikbaarheid verwys na die gemak waarmee tegnologiekoppelvlakke gebruik kan word, en word gereeld in die sagtewareontwikkelingsbedryf beoordeel en verbeter. Tog is dit nog nie die geval met e-leer nie, veral op die gebied van mediese onderrig. Swak bruikbaarheid beperk die moontlike voordeel van opvoedkundige hulpbronne, aangesien leerders voor die dubbele uitdaging van ’n ingewikkelde koppelvlak én die voorgeskrewe inhoud te staan kom. ’n Omvattende bruikbaarheidsbeoordeling is van die Electrolyte Workshop onderneem. Dit het toetsing met tipiese eindgebruikers ingesluit, waarvoor data met behulp van gestandaardiseerde vraelyste ingesamel en gebruikers se interaksie met die toepassing waargeneem en ontleed is. Ons het heuristiese evaluering as bykomende benadering gebruik en ’n kennerspaneel saamgestel om ons toepassing aan die hand van ’n stel heuristiek, oftewel beginsels van goeie koppelvlakontwerp, te beoordeel. ’n Hele aantal ernstige bruikbaarheidsprobleme is uitgewys, wat die moontlike opvoedkundige impak van die Electrolyte Workshop erg beperk. Daar was merkbare teenstrydigheid tussen die objektiewe bruikbaarheidsmaatstawwe en die selfaangemelde vraelysdata. Ons gebruikerstoetsdata lewer ’n waardevolle bydrae tot die debat oor hoeveel gebruikers nodig is om die meeste van die bruikbaarheidsprobleme met ’n koppelvlak te ontdek. Heuristiese evaluering was ʼn baie doeltreffende benadering. Tog het gebruikerstoetsing op sekere ernstige probleme afgekom wat heuristiese evaluering misgekyk het, en andersom. Hierdie beoordelings het as grondslag gedien vir ’n omvattende hersiening van die toepassing, waarna ons die oorspronklike weergawe in ’n verewekansigde proef met ’n verbeterde weergawe kon vergelyk. Die objektiewe bruikbaarheidsmaatstawwe het groot verbeterings getoon, wat waarskynlik leerders se tevredenheid en motivering sal verhoog. Leermaatstawwe het soortgelyke tellings opgelewer. Dít was egter te wagte gewees, aangesien die deelnemers almal betreklik ingelig was oor die vakmateriaal, eerder as nuwelinge. Ons studie het bevestig dat die bruikbaarheidsbeoordeling van e-leerhulpbronne noodsaaklik is, en bied ’n voorbeeld van hoe klinici-opvoeders bruikbaarder hulpbronne kan ontwikkel. Bruikbaarheid behoort as ’n roetinedeel van die ontwikkeling en inwerkingstelling van e-leermateriaal, -modules en -programme beoordeel te word. Dit behoort reeds by die vroegste weergawes van die hulpbron te begin, wanneer dit makliker en goedkoper is om veranderinge aan te bring. Ons het ook getoon dat ’n kombinasie van metodes gebruik behoort te word, en het die nut van heuristiese evaluering beklemtoon. ’n Herhalende benadering moet gevolg word, met etlike siklusse van toetsing en herontwerp. Gebruikerstoetsing behoort altyd die beoordeling van objektiewe bruikbaarheidsmaatstawwe in te sluit, en moenie slegs op selfaangemelde maatstawwe van gebruikerstevredenheid staatmaak nie.

Traditionally, volume has provided the link between trading activity and returns. This study attempts to not only investigate the trading behavior of all aspects of investors by daily order imbalances, the better index than dollar volume, around firm-specific news releases, but also explore the relation between order imbalances and daily returns. This study contributes to the shot-run market reactions and trading behaviours from different three or five kinds of investors around seasoned equity offerings announcement in Taiwan. We have examined 306 SEOs listed on Taiwan stock exchanges from 1995 to 1998, and test five subsequent SEO-related signaling dates, such as the shareholders’ conventions date, the formal announcement date, the ex-right date and the listed date. Our findings indicated the anomalies on returns and order imbalance did exist with the publication of SEO news in Taiwan. The negative information effect is significant on the shareholders’ convention date. Further we find a strong relation between order imbalance from individuals and daily return in the five day window. We infer that individual investors are extreme sensitivity to any news released and that the majority of traders in TSE are comprised by individual can explain the phenomenon. Finally, we also find not only correlation among different type of traders but also that returns, cash per share and the interest rate influence trading decision deeply. / Traditionally, volume has provided the link between trading activity and returns. This study attempts to not only investigate the trading behavior of all aspects of investors by daily order imbalances, the better index than dollar volume, around firm-specific news releases, but also explore the relation between order imbalances and daily returns. This study contributes to the shot-run market reactions and trading behaviours from different three or five kinds of investors around seasoned equity offerings announcement in Taiwan. We have examined 306 SEOs listed on Taiwan stock exchanges from 1995 to 1998, and test five subsequent SEO-related signaling dates, such as the shareholders’ conventions date, the formal announcement date, the ex-right date and the listed date. Our findings indicated the anomalies on returns and order imbalance did exist with the publication of SEO news in Taiwan. The negative information effect is significant on the shareholders’ convention date. Further we find a strong relation between order imbalance from individuals and daily return in the five day window. We infer that individual investors are extreme sensitivity to any news released and that the majority of traders in TSE are comprised by individual can explain the phenomenon. Finally, we also find not only correlation among different type of traders but also that returns, cash per share and the interest rate influence trading decision deeply.

Title: Compensation of moving system by football players Objectives: The aim of this work was detect muscle imbalances on fotball player's foots. By all the players was founded moving stereotype of hip extension too. Methods: In this thesis we used the methods of posttest with consecutive imperativ preexperiment. Obtained results of muscles imbalances and hip extension we comparated with results after two months ongoing compensation program. We detected effect of moving compensation program by the Cohen "effect size". We rated changes of muscles imbalances and hip enxtension. To obtaining results we used methods of dr. Janda. Results: After 2 moths long compensation moving program it got by the testing football players to positive changes on theirs muscle imbalances. Moving stereotyp of hip extension and size of testing muscles was after compensation moving program better. Compensation moving program was demonstrably effective. Keywords: moving stereotyp, compensation program, moving systém, muscle imbalances

Title: Effects of dance sport in the musculoskeletal system of the individual Objectives: The goal is to musculoskeletal examination of selected individuals and evaluation the effect of dance sport on examined status. The results should be the basis for further research in this area. Methods: Kinesiology analysis, bioimpedance analysis and X-ray were used for scrutiny of the musculoskeletal system. Primary and secondary data were analyzed afterwards. Results: Findings of struktural deformities: flat feet (both), both sided hallux valgus with incipient degenerative changes of I. MTP joint (male), limit angle of I. MTP joint of left leg (female). These deviations are related to the using of dance shoes. Muscle imbalance upper thoracic region and lateral imbalance of tested people are the result of the prescribed holding and leading used in dance. Keywords: dance sport, musculoskeletal system, muscle imbalances, feet deformities

This thesis employs two new approaches to explain some of the important debates in two key economic fields: labour market economics and macroeconomic studies related to current account imbalances. Chapter 1, Chapter 2 and Chapter 3 begin a new strand of research by introducing the normal inverse Gaussian (NIG) distribution to describe unobserved heterogeneity in the labour market. The NIG distribution can be represented as a normal variance-mean mixture with the inverse Gaussian (IG) distribution as the mixing distribution. A 0.01% subsample of the 1980 US Census, comprising all men between 18 and 65 who are in the labour force, as well as a comparable sample from Ghana, is used to show that the NIG distribution provides a better fit of the log earnings function than the normal distribution. The prediction of right skewness of the log earnings distribution arising from the log normal skill Roy selection model is rejected in favour of left skewness. The thesis then extends the model to describe the distribution of log earnings conditioned on education. The same two datasets (US males and Ghanaian males) are used for the empirical analysis. We find that, once the unobserved heterogeneity is accounted for, the return to education is almost flat for lower levels of education in Ghana, and then increases for education levels greater than ten years. One of the key differences between the two datasets is that skewness and unobserved heterogeneity is a function of education for Ghana but not for the US. The NIG framework is found to be a useful tool to model this heterogeneity. Chapter 4 uses a model that allows for a rich structure of age effects similar to those predicted by the life cycle theories to argue that the demographic shifts are partly responsible for the sustained rise in the US current account deficit and the rapid increase in China's current account surplus in the last decade. However, demographics do not have an impact on the long run equilibrium or level of current accounts. Rather, they are important determinants of the short run adjustment of current accounts to their equilibrium levels. In the next twenty years, the demographic shifts are likely to push towards further current account positive adjustments in China and current account negative adjustments in the US. Developing the infrastructure, financial markets, policy tools and regulatory settings to be able to cope with the excess capital flow remains an urgent task.

This thesis deals with global imbalances in the world economy. Chapter one focuses on balance of payments theory, savings and investment. It also specifies global imbalances and presents the most important periods of their development. Chapter two analyses main causes of global imbalances and discusses the relationship between imbalances and current economic crisis. Chapter three describes current development of global imbalances, anticipated development in future and analyses possible solutions at national and international level.

The aim of this thesis is to find a reason why the US current account is in deficit, which is in contradiction of theoretical expectation. Prevailing discusion is about savings glut and defects in monetary policy. In our opinion both sides ignore China`s rising influence and real exchange rate. Balassa-Samuelson`s effect predict real exchange rate appreciation in converging economies, as long as their real GDP grows. Analysis of real exchange rate of Renminbi shows contradiction with Balassa-Samuelson effect, which is attributed to conditions on Chinese labour market. Chinese internal imbalance has impacts on global economy and nonappreciating real exchange rate of Renminbi deforming international trade is one of the factors that causes US current account deficit.

Le système monétaire international articulé autour d'une monnaie cœur et de monnaies périphériques évoluant dans des régimes de change différents s'avère intrinsèquement instable et a favorisé la hausse des déséquilibres de comptes courants et la crise financière de 2007. La littérature traitant des comportements d'épargne laisse présager d'une reprise de ces déséquilibres et des risques qui en découlent. Afin de rechercher des voies de stabilisation politiquement réalisables, nous avons évalué les taux de change d'équilibre des pays les plus impliqués dans les déséquilibres par une approche positive afin d'obtenir des résultats acceptables par les responsables politiques. Notre travail montre que le taux de change n'a joué qu'un rôle modéré dans le développement des déséquilibres et la littérature montre qu'il n'a joué qu'un faible rôle dans leur ajustement.Par ailleurs, les politiques monétaires accommodantes mises en place suite à la crise économique font peser des risques sur la stabilité financière. Compte tenu de la multiplicité des régimes de change et de la difficulté de prévoir et de maitriser les variations des taux de change, les risques liés aux déséquilibres de comptes courants et à l'instabilité financière nécessitent d'être appréhendés par d'autres voies d'ajustement que celles du taux de change. Nous montrons la faisabilité de zones monétaires permettant l'applicabilité de politiques monétaires appropriées en cas d'asynchronisme des cycles économiques favorisant la mise en place de politiques budgétaires à la fois appropriées pour limiter la reprise de déséquilibres mais également contracycliques. / The International Monetary System built around a core currency, the US dollar and peripheral currencies working in various exchange rate regimes appears inherently unstable and has fostered the rise of global current account imbalances and the financial crisis in 2007. The literature on changes in savings behavior suggests a resumption of these imbalances and associated risks. Their partial adjustment would therefore only be cyclical, resulting from the economic crisis in 2008.In view of looking for politically achievable ways to stabilize imbalances, we evaluated the equilibrium exchange rate of the countries or regions most involved in the imbalances by a positive approach to achieve results acceptable by policymakers. Our work shows that the exchange rate has played a moderate role in the development of imbalances and recent literature suggests that it played only a minor role in their adjustment.Moreover, accommodative monetary policies implemented after the economic crisis constitute risks to financial stability. Given the multiplicity of exchange rate regimes and the difficulty to forecast and manage the changes in exchange rates, risks related to global current account imbalances and financial instability need to be tackled by other channels of adjustment than the exchange rate. Our work therefore concerns, in theory but also pragmatically, options that could both reduce the risk of financial instability and that of the resumption of global current account imbalances. We show the feasibility of monetary zones allowing the implementation of appropriate monetary policies in case of asynchrony of economic and financial cycles. We conclude with the stabilizing ro