Protein synthesis is principally regulated at the initiation stage in which eIF4F complex plays an important role. The eIF4F complex contains three subunits - eIF4A, eIF4E and eIF4G. The eIF4E is cap binding protein, the eIF4A is RNA dependent helicase which unwinds secondary structures at mRNA and scaffolding eIF4G protein. The interaction with other translation initiation factors is important for protein synthesis. The goal of my thesis was to create a new Saccharomyces cerevisiae yeast strain with the human eIF4F factor. Firstly I replaced yeast eIF4E protein with human eIF4E protein. I used a cre/loxP recombination to prepare yeast strains with deleted genes eIF4GI (huΔ4G1) and eIF4GII (huΔ4G2). Characterization of the new yeast strains showed that the human eIF4E protein replaced yeast ortholog factor better than the eIF4E protein from yeast Candida albicans. First experiments showed putative role of the eIF4GII protein during the cell growth under the temperature and osmotic stress. Key words: translation initiation, eIF4E, eIF4G, Saccharomyces cerevisiae

Master of Public Health - MPH / Adequate levels of adherence to treatment for tuberculosis (TB) and HIV at the same time poses a problem for public health in South Africa. TB/HIV co-infected patients face many potential barriers to adherence to treatment for both conditions. There is a need for more knowledge about factors influencing treatment adherence in co-infected patients on concomitant treatment. The aim of this study was to explore the barriers and facilitators to treatment adherence among people co-infected with TB/HIV living in the Alfred Nzo District, Eastern Cape, in order to identify the barriers and facilitators to adherence.

The switched telephony network was designed for voice calls. Expansion of datacommunication has lead to a wide range of experimentation to create new services. Theses services take place outside the network. When adding new services we currently encounter problems due to limitations of the simple devices at end points. Theo Kanter has proposed a new model to remove these limitations; this model is called “Adaptive Personal Mobile Communication”. The model consists of several components in the application layer of ISO standard. This model is based on peer to peer connections and the purpose of this model is to move services from within the networks to end point devices and avoid using central servers within the network. The Session Initiation Protocol (SIP) for establishing multimedia sessions allows us to move the point of integration for multimedia service integration out to the end-points. This project concerns the implementing of a prototype of this model as an SIP extension along with it evaluation. SIP offer addressing, naming, and localization of resources in this project. This report presents different design alternatives for XSP as an SIP extension, and the chosen model presents as a result of comparing of these design alternatives.

In this study, Mapulana male learners’ views on the influences of cultural initiation on their schooling were researched. Rite of passage was adopted as the theorisation for the study. In the context of the study, a rite of passage is when uninitiated male learners (mashoboro) go through the initiation process. Thereafter, their status of being boys is converted to becoming men (monna) through a transition (passage). Likewise, normal schooling is also a rite of passage, and Grades 1 to 12 are interrupted by another form of schooling, initiation, as a rite of passage. This study was motivated by the tension that was observed between the legal and customary, democratic and cultural, and formal and informal schooling. The study was qualitative in nature and rooted in the interpretive paradigm. Semi-structured interviews were employed to understand the Mapulana male learners’ views on the influences of cultural initiation on their schooling. It was found that the operation of initiation schools in Mpumalanga conflicted with the school calendar and this ultimately influenced schooling. Much of this could be attributed to the loopholes in policies governing initiations. This study can capacitate the Mpumalanga Department of Education (MDoE) about possible policy amendments. I recommend that parents raise this problem with principals through school governing bodies (SGBs) and that it be brought to the attention of the MDoE. The authorities should come to an agreement that prospective initiates should undergo medical circumcision before they go into cultural initiation performed in the bush. I also recommend that the above stakeholders should only allow male learners who are less than 15 years of age or those learners who are still in Grades 8 to 11 to undergo cultural initiation. The school management team (SMT) should help male learners who were away for initiation (initiates) with catch- up programmes. The MDoE must work hand-inglove with the Department of Health, Department of Cooperative Governance, Human Settlements and Traditional Affairs (CoGTA), and law enforcement agencies to prevent male Mapulana learners from being left behind in the curriculum. It must be ensured that learners’ health comes first and that initiation principals strictly adhere to policies governing initiation schools. / Dissertation (MEd)--University of Pretoria 2020. / pt2021 / Humanities Education / MEd / Unrestricted

Background: T2DM patients can be treated with both Insulin or/and non-insulin antidiabetic drugs. Biguanide-derivate is the first-line antidiabetic that is cost-effective and safe. DPP-4Is are antidiabetic drugs that can be used to treat old patients and those with kidney failure. While SGLT2-inhibitors and GLP-1analogues can be used by T2DM patients with cardiovascular problems and those with overweight. The initiation of antidiabetic drug is influenced by several factors such as patient characteristic, physician’s knowledge and guidelines. Aim: The aim of this study was to study the type of antidiabetic medication initiated to naïve T2DM patients, how age and sex affect the choice of medication and difference in prescribing between Primary healthcare centers in Region Uppsala. Methods: The study was a cross-sectional based on health record data from Region Uppsala. The study included 2473 T2DM patients that were initiated antidiabetic medication between January 2019 and December 2020. Results: Non-insulins accounted for 88 % and insulins for 21.1% of the prescriptions. The proportion of men that received both insulin and non-insulins were higher than women. The proportion of patients that received insulins was higher for the age group 80 years and older. The proportion of men that received SGLT2- inhibitors was higher than for women, while women received more GLP-1- analogues. There was a variation between PHCs, but non-insulins were mostly prescribed at all PHCs. Conclusions: Initiation of antidiabetic drugs to naïve T2DM patients in region Uppsala fallowed the guidelines. There are some differences in prescription pattern between the two sexes and the age groups.

Submitted in partial fulfilment of the requirements for The Degree of Doctor of Philosophy (Community Psychology) Faculty of Arts University of Zululand, 2010. / The lives of individuals in all societies are a series of passages from one age to another and from one occupation to the other. Among the Vhavenda, there are fine distinctions among age or occupational groups and progression from one group to the next is accompanied by special rituals enveloped in ceremonies which involve actions that are clearly regulated and guarded so that the entire society suffer no discomfort or injury. The research explores and describes the lived experiences of Vha-Venda initiated women in the rural areas of Mashau, Mashawana and Shayandima village in Limpopo Province, South Africa. The perception that transition practices, otherwise known as initiation rituals or rites of passage, are only practiced in the ‘traditional’ societies because it is believed to be where the culture is embedded. This study draws on qualitative research principles based on the ethnographic approach. This research explores and describes the lived experiences of initiation of Venda women that is practiced and currently being implemented in the three villages that is, Mashau, Mashawana and Shayandima village in Limpopo Province, South Africa. As a result, this study is informed by the qualitative data gathered during the initial stages of the research with the assistance of research guides. The core material in this study emerges from in depth, semi-structured interviews conducted during individual interviews and focus group interviews with fifteen initiated women and two research guides between the age of thirty and sixty. The research guides, with special knowledge of the culture assisted the researcher on the process and activities of the initiation and also informed the researcher about the venues where certain rituals take place and also assisted in translation of certain phrases for clarification. In compliance with research ethics, the identities of the respondents remain confidential through the use of pseudonyms. The research concludes that the lived experiences of women initiation are private and one is strictly prohibited to talk about them, especially with uninitiated women. The aim is to portray the traditional social and cultural ritual proposed to be learnt and preserved. In this study, the researcher’s findings are that: Conformity, compliance and obedience with the initiation rituals can save a person from embarrassment in Limpopo Province where initiation is practiced. Participants reported that women are silenced and forbidden to talk about initiation outside ‘dombani’ with the uninitiated women. They reported that the initiation ritual is secret and thus a taboo to talk about it. Initiates are prohibited to disclose what happens during the initiation process. In contrast, uninitiated women viewed the ritual as barbaric and promiscuous. The initiated indicated that they were forced to attend because of fear of rejection, discrimination and isolation by the community. Other participants agreed to have attended for the sake of acceptance, though they believed to have gained knowledge about understanding womanhood. Most of the women mentioned that although it was some years that they had attended the initiation school, they still carried the burden of anger, shame, humiliation, frustration, low-self esteem, sense of helplessness and lack confidence and still find it hard to share their experiences or talk about them. The researcher concurs with the participants and Stayt (1968) that initiated women are denied freedom of expression. It is sticky prohibited to talk or share the initiation experiences with the non- initiated let alone discuss it outside dombani. Thereby, the aim, and its concomitant 4 objectives, have been thoroughly explored and achieved.

Gait initiation (GI) is an important locomotor transition task that includes anticipatory postural adjustments and the joint propulsion necessary for the first step of walking. Metrics associated with this task are known to change across the lifespan and may provide valuable information for fall risk indication, as falls often occur during transitional tasks. Assessments of discrete variables between fallers and non-fallers at GI have provided insight into differences between groups. However, more complex approaches such as time-series principal component analysis (PCA) may allow the examination of changes in magnitude, pattern, and timing not detectable using discrete comparisons alone. Therefore, this thesis aims to characterize differences between fallers and non-fallers by examining the kinematics and kinetics of gait initiation using time-series PCA. A sample of 56 community-dwelling older adults was recruited for this study and completed five walking trials where GI was measured by two force platforms. PCA of centre of pressure kinematics and kinetics time-series data were used to identify the critical features of the signal, and multivariate analysis of covariance was used to compare the individual loading scores of each principal component for each phase between groups. It was revealed that fallers demonstrated differences in the range of mediolateral movement during weight transfer and forward progression, a greater range of anteroposterior movement in forward progression, and a more gradual rise in vertical forces in the first step, associated with a shorter first step length. These findings point to a tendency for fallers to prioritize stability over forward progression performance, and differences in postural control strategies, compared to non-fallers. Further, the use of time-series PCA helped to highlight differences not detectable using discrete analysis alone. / Graduate

In this study, the effects of stress and strain rate on cyclic deformation, secondary hardening, martensitic phase transformation, crack initiation, and fatigue behavior of type 304L stainless steel are examined. A series of load and strain controlled uniaxial zero and non-zero mean strain fatigue tests were conducted with varying frequencies in order to investigate the effect of loading rate on fatigue behavior. The volume fraction of martensite was quantified for several tests using x-ray diffraction and electron backscatter diffraction. The loading rates were found to have a direct effect on the microstructure and fatigue behavior of the alloy investigated. Adiabatic heating from an increased rate of loading was found to effect martensite formation which is a major contributor to the secondary hardening phenomena associated with many austenitic stainless steels under cyclic loading. Also affected by the microstructural changes were cyclic deformation, crack initiation, microstructurally small crack growth, and fatigue behavior.

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