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The pathology of tuberculosis, caused by mycobacterium tuberculosis, in a herd of semi free-ranging springbok (Antidorcas marsupialis)Gous, Tertius A. 05 May 2008 (has links)
This first detailed description of the pathology of tuberculosis, caused by Mycobacterium tuberculosis in springbok is reported. The springbok were part of a semi free-ranging herd kept on the grounds of iThemba Laboratory for Accelerator Based Science (LABS) in the Kuils River district of the Western Cape Province, South Africa. Of the 33 animals sampled, two animals had tuberculosis lesions. Mycobacterium tuberculosis was isolated from these two animals, as well as an animal that did not show tuberculosis pathology. The index case was an adult ewe that was presented for necropsy in a severely weakened condition. The ewe showed advanced miliary tuberculosis with marked macroscopic lesions in the lungs, pleura and respiratory lymph nodes. Limited sampling was done but microscopic tuberculosis lesions were found in almost all the organs sampled, and acid-fast bacilli were generally numerous. Six healthy rams were culled nine months later and a pilot study indicated miliary tuberculosis lesions in one ram, which again were macroscopically most prominent in the lungs, pleura and respiratory lymph nodes. Macroscopic lesions were also noted in the sternal, iliac, prefemoral and retropharyngeal lymph nodes. Microscopy in this animal revealed lesions in the macroscopically affected organs as well as numerous other lymph nodes, and suspected lesions occurred in the testicle and colon. Acid-fast bacilli were scarce to moderate in affected organs. Because of the miliary nature of the lesions in both affected animals, the route of infection could not be established conclusively. The lesions in most affected organs of both animals resembled classical tuberculous granulomas, viz. central caseous necrosis, with various degrees of calcification, surrounded by various numbers macrophages, epithelioid cells, multinucleated giant cells and lymphoplasmacells, and mild to moderate fibrous encapsulation. Necrotic lesions in the spleen, liver and kidney of the ewe were more disseminate and coagulative. A main study conducted on healthy culled animals 19 months after the pilot study failed to find any animal with tuberculosis lesions in the group of 25 sampled. These animals were all negative for mycobacteria via mycobacterial culture. The Interferon-gamma (INFg) assay was performed on all the animals of the pilot and main study but failed to identify the culture-positive animals and showed one false-positive reaction. / Dissertation (MMedVet (Pathology))--University of Pretoria, 2007. / Paraclinical Sciences / unrestricted
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Development of ELISAs for the detection of interferon-gamma in rhinoceroses and elephants as diagnostic tools for Mycobacterium bovis and Mycobacterium tuberculosis infectionsMorar, Darshana 03 December 2009 (has links)
Please read the abstract in the 00 front of this document. / Thesis (PhD)--University of Pretoria, 2009. / Veterinary Tropical Diseases / unrestricted
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An experimental study in the use of instrumentation to analyze metabolism and product formation in cell cultureFleischaker, Robert James January 1982 (has links)
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1982. / MICROFICHE COPY AVAILABLE IN ARCHIVES AND SCIENCE. / Pages 373-383 reprint from Developments in Industrial Microbiology. / Bibliography: p. 243-261. / by Robert James Fleischaker, Jr. / Ph.D.
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The identification and characterization of novel persistence genes in chlamydia trachomatisMuramatsu, Matthew Kazuyuki 30 November 2016 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Chlamydia trachomatis is an obligate intracellular bacterial pathogen that
can infect the eyes, genital tract, and disseminate to lymph nodes in humans.
Many C. trachomatis infections are clinically asymptomatic and can become
chronic if left untreated. When humans are infected with C. trachomatis, a
cytokine that is produced is interferon-gamma (IFN-γ). In vitro, IFN-γ stimulates
expression of the host enzyme indoleamine 2,3-dioxygenase. This enzyme
converts free intracellular tryptophan to N-formylkynurenine. Tryptophan
starvation induces C. trachomatis to enter a viable-but-nonculturable state
termed persistence, which has been proposed to play a key role in chronic
Chlamydial disease. To circumvent host induced tryptophan depletion,
urogenital strains of C. trachomatis encode a functional tryptophan synthase
(TS). TS synthesizes tryptophan from indole and serine, allowing Chlamydia to
reactivate from persistence. Transcriptomic analysis revealed C. trachomatis
differentially regulates hundreds of genes in response to tryptophan starvation.
However, genes that mediate entry, survival, and reactivation from persistence
remain largely unknown. Using a forward genetic screen, we identified six
Susceptible to IFN-γ mediated Persistence (Sip) mutants that have diminished
capacities to reactivate from persistence with indole. Mapping the deleterious
persistence alleles in three of the Sip mutants revealed that only one of the
mutants had a mutation in TS. The two other Sip mutants mapped had mutations in CTL0225, a putative integral membrane protein, and CTL0694, a
putative oxidoreductase. Neither of these genes plays a known role in
tryptophan synthesis. However, amino acid (AA) competitive inhibition assays
suggest that CTL0225 may be involved in the transport of leucine, isoleucine,
valine, cysteine, alanine, and serine. Additionally, metabolomics analysis
indicates that all free amino acids are depleted in response to IFN-γ, making this
amino acid transporter essential during persistence. Taken together we have
identified two new chlamydial persistence genes that may play a role in chronic
chlamydial disease.
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β-Cell Autophagy in the Pathogenesis of Type 1 DiabetesMuralidharan, Charanya 12 1900 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Type 1 diabetes (T1D) is a multifactorial disease involving genetic and environmental factors. One of the factors implicated in disease pathogenesis is early life viral infection. A typical immune response to viral infection includes production of type 1 interferons (IFN), such as IFN-α, which can induce stress in the pancreatic β-cells. Reactive oxygen species (ROS) accumulation occurs after exposure to other inflammatory cytokines, causing oxidative stress that may be linked to T1D pathogenesis. Therefore, we hypothesized that IFN-α may also elicit β-cell ROS accumulation. Our in vivo and in vitro experiments with human islets showed rapid and heterogenous ROS accumulation with IFN-α. Although T1D is characterized by autoimmune destruction of β-cells, some cells survive this persistent attack. We hypothesized that survival/ death of β-cells could be attributed to the ability to effectively mitigate ROS accumulation.
One mechanism to mitigate ROS is autophagy, which degrades and recycles cellular components to promote cellular homeostasis. We observed an impairment in autophagy in β-cells of donors with T1D as well as in islets of diabetic non-obese diabetic (NOD) mouse model of autoimmune diabetes. Autophagic flux was also impaired in diabetic NOD mouse islets, further confirming impairment of autophagy. Interestingly, we observed an induction of autophagy after acute treatment with IFN-α both in vitro and in vivo, suggesting compensatory upregulation of autophagy to restore homeostasis. Similarly, we observed an increase in autophagosomes and telolysosomes in β-cells of normoglycemic autoantibody positive organ donors compared to nondiabetic organ donors. Together, these data implicate a defect in the final degradation step of autophagy involving lysosomes. Therefore, we analyzed the activity and expression of lysosomal cysteine protease Cathepsin H (CTSH, a T1D susceptibility locus), and found both to be increased in islets of pre-diabetic NOD mice. Together, these data support compensatory hyperactivation of lysosomal enzymes prior to overt diabetes, potentially to rid the cell of ROS and degradation-resistant oxidized proteins and lipids. We also observed that C57Bl/6J mice lacking a key autophagy enzyme, ATG7, in their β-cells, spontaneously developed hyperglycemia. Collectively, these data highlight the importance of -phagic degradation process in the pathogenesis of T1D. / 2022-12-28
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Cell polarity in hematopoietic stem cell quiescence, signaling and fate determinationAlthoff, Mark J. 02 June 2020 (has links)
No description available.
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Comparison of first-line therapies for relapsing-remitting multiple sclerosisYennam, Amulya 02 March 2021 (has links)
Multiple Sclerosis (MS) is a chronic and potentially disabling disease of the central nervous system (CNS) in which the immune system attacks the protective myelin layer that surrounds nerve cells. While the majority of individuals diagnosed with MS initially present with a non-progressive relapsing form of the disease, there is significant risk of eventually transitioning to a more progressive form for which there are few effective treatments. Consequently, early intervention with disease-modifying therapies (DMTs) is essential for effective disease management. Newly diagnosed patients are typically started on one of four first-line therapies (beta interferon, glatiramer acetate, teriflunomide, or dimethyl fumarate). Though there are distinct differences between these treatments in regard to efficacy and safety, there is no uniform standard for making decisions about which to initiate treatment with. This review gives an overview of current first-line MS therapies, and seeks to highlight the lack of comparison data and the gaps in the current understanding of disease management, as well as the need for more comprehensive research in these areas.
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Effects of Different Oral Doses of Cyclosporine on T-Lymphocyte Biomarkers of Immunosuppression in Normal DogsArcher, Todd Marlow 12 May 2012 (has links)
Cyclosporine is a potent immunosuppressive agent used to treat a wide range of canine inflammatory diseases. Unfortunately, optimal dosing protocols for achieving immunosuppression with cyclosporine in dogs remain unclear, and standard methods that objectively monitor effectiveness of immunosuppression have not been established. We evaluated an already established panel of biomarkers of immunosuppression in vivo with two oral dosages of cyclosporine in seven normal dogs, a high dosage known to induce immunosuppression and a lower dosage used to treat atopy, with a washout period between the two dosages. The biomarker panel included the flow cytometric evaluation of T-lymphocyte cytokine expression (IL-2, IL-4, and IFN-gamma). High dosage cyclosporine resulted in significant decreases in IL-2 and INF-gamma expression, but not IL-4 expression. Low dosage cyclosporine was associated with a significant decrease in INF-gamma expression, while IL-2 expression was not affected. The results demonstrated suppression of biomarkers in a dose-dependent manner.
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Interferon Regulatory Factors and Autoimmune DiseasesNing, Shunbin 01 January 2014 (has links)
No description available.
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Evaluation of the Role of Type-1 Interferon Signaling in the Pathogenesis of Salmonella TyphimuriumVerma, Priya 06 July 2022 (has links)
Innate immunity operates independently of prior exposure to pathogens. There are several signal transduction pathways that play a key role in inflammatory and immune responses. Critical signaling cascade in the interest of my research is type-1 interferon (IFN) signaling pathway in response to infection with Salmonella Typhimurium (ST). The role of type-I interferons is well established in the context of a viral infection; however, their role in bacterial infections is not clear. In my thesis I aimed to understand the role of type-1 IFNs in bacterial pathogenesis, and scrutinize the mechanism adopted by various components of type-1 IFN signaling, especially ISGF3 complex in response to Salmonella Typhimurium. My results indicate that type-I IFN signaling is detrimental to host survival. I further investigated the mechanism through which type-1 IFN signaling results in host susceptibility against Salmonella. My results indicated that the three transcription factors downstream of IFNAR1 have different impacts in mounting an innate immune response against ST. IRF9 and STAT2 promote susceptibility against ST whereas STAT1 through IFNAR1-signaling, promotes enhanced expression of pro inflammatory cytokines and protection against ST. I also observed that the monocytes/macrophages lineage in Ifnar1⁻ᐟ⁻ mice is responsible for conferring the enhanced resistance against ST. Furthermore, my work determined that expression of type-I IFN signaling compromises the fitness of macrophages by reducing mitochondrial respiration, glycolysis and myelopoiesis.
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