Global ETD Search

11	Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil Shorter, K., Farjo, N.P., Picksley, Stephen M., Randall, Valerie A. January 2008 (has links) No / Hair disorders cause psychological distress but are generally poorly controlled; more effective treatments are required. Despite the long-standing use of minoxidil for balding, its mechanism is unclear; suggestions include action on vasculature or follicle cells. Similar drugs also stimulate hair, implicating ATP-sensitive potassium (K(ATP)) channels. To investigate whether K(ATP) channels are present in human follicles, we used organ culture, molecular biological, and immunohistological approaches. Minoxidil and tolbutamide, a K(ATP) channel blocker, opposed each other's effects on the growing phase (anagen) of scalp follicles cultured in media with and without insulin. Reverse transcriptase-polymerase chain reaction identified K(ATP) channel component gene expression including regulatory sulfonylurea receptors (SUR) SUR1 and SUR2B but not SUR2A and pore-forming subunits (Kir) Kir6.1 and Kir6.2. When hair bulb tissues were examined separately, epithelial matrix expressed SUR1 and Kir6.2, whereas both dermal papilla and sheath exhibited SUR2B and Kir6.1. Immunohistochemistry demonstrated similar protein distributions. Thus, human follicles respond biologically to K(ATP) channel regulators in culture and express genes and proteins for two K(ATP) channels, Kir6.2/SUR1 and Kir6.1/SUR2B; minoxidil only stimulates SUR2 channels. These findings indicate that human follicular dermal papillae contain K(ATP) channels that can respond to minoxidil and that tolbutamide may suppress hair growth clinically; novel drugs designed specifically for these channels could treat hair disorders. ATP-binding cassette transporters Hair follicle Chemistry Humans Immunohistochemistry KATP channels Analysis Drug effects Genetics Minoxidil Pharmacology Organ culture techniques Polymerase chain reaction Potassium channels Inwardly rectifying Receptors Sulfonylurea receptors Tolbutamide Vasodilator agents REF 2014
12	Altered Skeletal Muscle Excitation-Contraction Coupling in the R6/2 Transgenic Mouse Model for Huntington's Disease Miranda, Daniel R. January 2021 (has links) No description available. Biomedical Research Biology Physiology action potential duration prolonged calcium Ca2+ force excitation-contraction coupling EC coupling chloride potassium Cl- K+ inwardly rectifying Kir ClC-1 skeletal muscle Huntington's disease huntingtin release flux voltage gated KV KV1.5 KV3.4 trains electrophysiology current-clamp
13	Analysis of mouse models of insulin secretion disorders Kaizik, Stephan Martin January 2010 (has links) No description available. 616.4

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