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Studies of electrospray /Ding, Luyi, January 1900 (has links)
Thesis (Ph. D.)--Carleton University, 2000. / Also available in electronic format on the Internet.
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Investigation of large protein and multimeric protein complex structures with mass spectrometry techniquesPacholarz, Kamila Jolanta January 2015 (has links)
The biophysical properties, biological activity and function of macromolecular systems are highly dependent on their structure. Structure-activity relationships of proteins and their binding partners are critical for drug discovery, biochemical and medical research. While the gas-phase environment might present as an unusual venue from which to explore protein structure, for over the past two decades, nano-electrospray ionization (nESI) coupled to mass spectrometry (MS) has been recognized as having great potential for analysis of protein structure and protein non-covalent complexes. In conjunction with related technique of ion mobility (IM), mass spectrometry (IM-MS) provides insights into protein native-like conformations and any structural changes in may undergo upon ligand binding or alternations induced via physical parameters such as temperature, pressure or solution conditions. As most proteins tend to exist as multiple domains; from the distribution of oligomeric states in the Protein Data Base (PDB) 86% of proteins exist as oligomers; the work presented in this thesis focuses on application of MS techniques to probe the tertiary and quaternary structure of various large and multimeric protein complexes, their dynamics and/or conformational changes. Wherever relevant, the gas-phase studies reported here are complemented by other techniques, such as hydrogen deuterium exchange MS (HDX), molecular modelling (MD) and analytical ultracentrifugation (AUC). Firstly, the dynamics of intact monoclonal antibodies (mAbs) and their fragments are explored with IM-MS. Variations observed in conformational landscapes occupied by two mAb isotypes are rationalized by differences in disulfide linkages and non-covalent interactions between the antibody peptide chains. Moreover, mAb intrinsic flexibility is compared to other multimeric protein complexes in terms of collision cross section distribution span. Secondly, variable temperature MS (VT-MS) and variable temperature IM-MS (IM-MS) are used to probe unfolding and dissociation of four standard multimeric protein complexes (TTR, avidin, conA and SAP) as a function of the of analysis environment temperature. VT-MS is found to allow for decoupling of their melting temperature (Tm) from the protein complex dissociation temperature (TGPD). Whereas, VT-IM-MS is used to investigate structural changes of these protein complexes at elevated temperatures and provide insights into the thermally induced dissociation (TID) mechanism, as well as strength of the non-covalent interactions between subunits. Thirdly, VT-(IM)-MS methodology is applied to study behaviour of three mAbs: IgG1, IgG4 and an engineered IgG4 of increased thermal stability. Such analysis shows to be promising for comparative thermal stability studies for proteins of therapeutic interest. Lastly, the structure of ATP-phosphoribosyltransferase (MtATPPRT), an enzyme catalysing the first step of the biosynthesis of L-histidine in Mycobacterium tuberculosis, is explored. Conformational changes occurring upon feedback allosteric inhibition by L-histidine are probed with MS, IM-MS, HDX-MS and AUC. Reported results serve as the basis for IM-MS/HDX-MS based screening method to be used for screening of a library of novel and promising anti-tuberculosis agents.
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Comprehensive Analysis of Emerging New Psychoactive Substances by Ion Mobility Spectrometry and Mass SpectrometryGwak, Seongshin 17 September 2015 (has links)
In the new era of drug abuse, the proliferation of new psychoactive substances (NPS), commonly referred to as designer drugs or legal highs, has been a global concern. These substances are produced to circumvent current legislation for controlled substances with minor modifications in their chemical structure. Although many efforts have been made previously, the characterization of such substances are still challenging because of (1) the continual emergence of newly identified substances, (2) the lack of a universal screening test for NPS that are structurally similar to each other, and (3) the complex and time-consuming chromatographic techniques currently used. Therefore, it is necessary to develop novel analytical methods that can be readily adapted by forensic laboratories to overcome these challenges.
In this dissertation, various analytical techniques have been evaluated for qualitative analysis of these emerging NPS. For rapid screening purposes, a commercial ion mobility spectrometry with a 63Ni ion source (63Ni-IMS) and also direct analysis in real time coupled to a quadrupole time-of-flight mass spectrometer (DART-QTOF-MS) were investigated first. The results showed that rapid detection by 63Ni-IMS and identification by DART-QTOF-MS can be achieved with sub-nanogram detection capability and high speed total analysis time less than two minutes. In recent developments of gas chromatography mass spectrometry (GC-MS), gas chromatography (GC) has been coupled to state-of-the-art mass spectrometers, including triple quadrupole (MS/MS) and quadrupole time-of-flight (QTOF). It was revealed that the application of GC-MS/MS and GC-QTOF facilitates the unambiguous identification of emerging NPS with a chemical ionization (CI) source. In addition, constitutional isomers of NPS were differentiated with the capabilities of product ion scan and multiple reaction monitoring (MRM) modes. Finally, the coupling of IMS with a mass spectrometer (IMS-MS) was investigated as an alternative confirmatory technique. With the development of an optimal solvent system in the electrospray ionization (ESI) process, the rapid analysis and identification of synthetic cathinone was successfully achieved less than five minutes. As a proof-of-concept, seized drugs samples provided by a local forensic laboratory were analyzed using these developed methods by various analytical techniques. The results from these seized samples are also presented in this evaluation.
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Headspace Analysis of Smokeless Powders: Development of Mass Calibration Methods using Microdrop Printing for Chromatographic and Ion Mobility Spectrometric DetectionJoshi-Kumar, Monica 25 March 2010 (has links)
Smokeless powder additives are usually detected by their extraction from post-blast residues or unburned powder particles followed by analysis using chromatographic techniques. This work presents the first comprehensive study of the detection of the volatile and semi-volatile additives of smokeless powders using solid phase microextraction (SPME) as a sampling and pre-concentration technique. Seventy smokeless powders were studied using laboratory based chromatography techniques and a field deployable ion mobility spectrometer (IMS). The detection of diphenylamine, ethyl and methyl centralite, 2,4-dinitrotoluene, diethyl and dibutyl phthalate by IMS to associate the presence of these compounds to smokeless powders is also reported for the first time. A previously reported SPME-IMS analytical approach facilitates rapid sub-nanogram detection of the vapor phase components of smokeless powders. A mass calibration procedure for the analytical techniques used in this study was developed. Precise and accurate mass delivery of analytes in picoliter volumes was achieved using a drop-on-demand inkjet printing method. Absolute mass detection limits determined using this method for the various analytes of interest ranged between 0.03 - 0.8 ng for the GC-MS and between 0.03 - 2 ng for the IMS. Mass response graphs generated for different detection techniques help in the determination of mass extracted from the headspace of each smokeless powder. The analyte mass present in the vapor phase was sufficient for a SPME fiber to extract most analytes at amounts above the detection limits of both chromatographic techniques and the ion mobility spectrometer. Analysis of the large number of smokeless powders revealed that diphenylamine was present in the headspace of 96% of the powders. Ethyl centralite was detected in 47% of the powders and 8% of the powders had methyl centralite available for detection from the headspace sampling of the powders by SPME. Nitroglycerin was the dominant peak present in the headspace of the double-based powders. 2,4-dinitrotoluene which is another important headspace component was detected in 44% of the powders. The powders therefore have more than one headspace component and the detection of a combination of these compounds is achievable by SPME-IMS leading to an association to the presence of smokeless powders.
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Putting the Pieces Together Again: Characterizing Trisaccharides by the Energetics of Their Primary Fragmentation Pathways and Their Ion MobilityOverton, Sean 10 November 2021 (has links)
Identification of polysaccharides is not a straightforward task due to the high degree of stereochemistry present in their isobaric monomers. Their isobaric nature causes traditional mass spectrometry to fall short when trying to differentiate not only the conformation of the monomers but the position of the glycosidic bonds that bind them. This structural information is important for biochemists as they study the role of different glycans in biological processes.
Tandem mass spectrometry (MS/MS) allows the study of the fragment ions formed during collision induced dissociation (CID), the fragments formed depend on the structure and stability of the precursor molecule and can be used to identify the compounds. These fragmentation pathways will be as complex as the species that form them. To date, typical saccharide fragments are separated into three groups that represent the major fragments: Cross-ring cleavages (A/X), and those resulting from cleaving different sides of the glycosidic bond (B/Y) and (C/Z).
Ion mobility separation (IMS) has shown to have some success at discerning polysaccharide conformers and those of other biopolymers such as proteins and polynucleotides. Ion mobility separates gas-phase ions by colliding them with non-reactive gases and relating respective increase in flight time to their collision cross-section (CCS).
In this study, the relative energetics of the first steps of the cross-ring cleavage and both glycosidic bond cleavage channels for isomaltotriose [glc(α1-6)glc(α1-6)glc] as well as a minor water loss channel were explored using density functional theory (DFT) calculations at the B3LYP/6-31+g(d) level of theory. It was demonstrated that charge-remote mechanisms are a viable alternative to charge-directed mechanisms when under the high energy short time scale conditions present during an ESI-MS/MS experiment.
To verify the efficiency of ion mobility for isomeric separation, the relative experimental CCS of isomaltotriose [glc(α1-6)glc(α1-6)glc], maltotriose [glc(α1-4)glc(α1-4)glc], panose [glc(α1-6)glc(α1-4)glc] and raffinose [gal(α1-6)glc(α1-2)fru] were determined by comparison with literature CCS values for dextran, a variable-length oligomer of α1-6 linked glucose was used as an external calibrant. The experimental CCS of the precursor ions were compared to literature values when available as well as the calculated effective values of the optimized DFT geometries using the trajectory method of the MOBCAL computational suite.
As phosphate is often used as an adducting agent to increase the intensity of the precursor ion when running an IMS experiment, the effect of its presence on the fragmentation of isomaltotriose and large isomaltooligosaccharides was studied. It was seen that depending on the location of the phosphate ion, it will preferentially dissociate leaving behind a neutral glycan. This explains the low abundance of fragment ions observed when selecting a phosphate-adducted precursor ion during an MS/MS experiment.
IMS and MS-MS are complementary methods that can be used to identify monomers within a polysaccharide and how they are bound.
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Applications of Metallic Clusters and Nanoparticles via Soft Landing Ion Mobility, from Reduced to Ambient PressuresAguilar Ayala, Roberto 08 1900 (has links)
Nanoparticles, simple yet groundbreaking objects have led to the discovery of invaluable information due to their physiological, chemical, and physical properties, have become a hot topic in various fields of study including but not limited to chemistry, biology, and physics. In the work presented here, demonstrations of various applications of chemical free nanoparticles are explored, from the determination of a non-invasive method for the study of the exposome via using soft-landing ion mobility (SLIM) deposited nanoparticles as a matrix-assisted laser desorption/ionization (MALDI-MS) matrix replacement, to the direct SLIM-exposure of nanoparticles onto living organisms. While there is plenty of published work in soft-landing at operating pressures of 1 Torr, the work presented here shows how this technology can be operated at the less common ambient pressure. The ease of construction of this instrument allows for various modifications to be performed for a wide array of applications, furthermore the flexibility in metallic sample, operating pressure, and deposition time only open doors to many other future applications. The work presented will also show that our ambient SLIM system is also able to be operated for toxicological studies, as the operation at ambient pressure opens the door to new applications where vacuum conditions are not desired.
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Ion mobility mass spectrometry as a powerful tool to analyze complex macromolecular systemsFrerichs, Niklas 19 January 2021 (has links)
No description available.
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Modeling and experimenting a novel inverted drift tube device for improved mobility analysis of aerosol particlesNahin, Md Minal 12 1900 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Ion Mobility Spectrometry (IMS) is an analytical technique for separation of charged particles in the gas phase. The history of IMS is not very old, and in this century, the IMS technique has grown rapidly in the advent of modern instruments. Among currently available ion mobility spectrometers, the DTIMS, FAIMS, TWIMS, DMA are notable. Though all the IMS systems have some uniqueness in case of particle separation and detection, however, all instruments have common shortcomings. They lack in resolution, which is independent of mobility of different charged particles and they are not able to separate bigger particles (20 120 nm) with good accuracy. The work presented here demonstrates a new concept of IMS technique at atmospheric pressure which has a resolution much higher than that of the currently available DTIMS (Drift Tube Ion Mobility Spectrometry) instruments. The unique feature of this instrument is the diffusion auto-correction. Being tunable, It can separate the wide range of particles of different diameters. The working principle of this new IMS technique is different from the typical DTIMS and to simply put, it can be considered as an inversion of commonly used technique, so termed as Inverted Drift Tube (IDT).The whole work performed here can be divided into three major phases. In the first phase, the analytical solution was derived for two new separation techniques: IPF (Intermittent push flow) and NSP (Nearly stopping potential) separations. In the next phase, simulations were done to show the accuracy of the analytical solution. An ion optics simulator software called SIMION 8.1 was used for conducting the simulation works. These simulations adopted the statistical diffusion (SDS) collision algorithm to emulate the real scenario in gas phase more precisely. In the last phase, a prototype of experimental setup was built. The experimental results were then validated by simulated results.
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Ion Mobility Spectrometry : Optimization of Parameters in Collision Cross Sections and Trace Detection of ExplosivesWu, Tianyang 12 1900 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Ion mobility spectrometry is a powerful technique for the study related to molecule. The work of tow major applications are introduced in this paper. The first application is the optimization of parameters in CCS. The accurate calculation of the collision cross section for multiple molecules is a long-time interested topic in the research for substances detection in micro scale. No reliable analytical approach to calculate the collision cross section has been established to date. Different approaches rely on different mechanism will provide different results in significant extent. This work introduce a method for the determination of parameters in the Lennard Jones potential. Experimental data combined with numerical computation was the fundamental strategy during the optimization of the parameters. In the experiment, electrospray is used as the ion source of IMS while a nebulizer was utilized to electrify the aromatic compounds. New parameters show no less accuracy and equal efficiency while can explain the physical meaning of the collision more clearly. The second application is the trace detection of explosives with very low concentration. The detection of explosives is an important topic in security, while the detection will be difficult due to the low vapor pressure of explosives. In this work, two types of devices are designed for the trace detection of explosives at an extremely low concentration. TNT is selected as the explosives in the experiment. The experiment succeed to reach a sensitivity of 1 part per quintillion, and even find out a linear relationship between the logarithm of TNT concentration and TNT vapor pressure.
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Ion Mobility and Gas-Phase Covalent Labeling Study of the Structure and Reactivity of Gaseous Ubiquitin Ions Electrosprayed from Aqueous and Denaturing SolutionsCarvalho, Veronica Vale 12 1900 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Gas-phase ion/ion covalent modification was coupled to ion mobility/mass spectrometry
analysis to directly correlate the structure of gaseous ubiquitin to its solution structures with selective covalent structural probes. Collision cross-section (CCS) distributions were measured prior to ion/ion reactions to ensure the ubiquitin ions were not unfolded when they were introduced to the gas phase. Ubiquitin ions were electrosprayed from aqueous and methanolic solutions yielding a range of different charge states that were analyzed by ion mobility and time-of-flight mass spectrometry. Aqueous solutions stabilizing the native state of ubiquitin generated folded ubiquitin structures with CCS values consistent with the native state. Denaturing solutions favored several families of unfolded conformations for most of the charge states evaluated. Gas-phase covalent labeling via ion/ion reactions was followed by collision-induced dissociation of the intact, labeled protein to determine which residues were labeled. Ubiquitin 5+ and 6+ electrosprayed from aqueous solutions were covalently modified preferentially at the lysine 29 and arginine 54 residues, indicating that elements of secondary structure, as well as tertiary structure, were maintained in the gas phase. On the other hand, most ubiquitin ions produced in denaturing conditions were labeled at various other lysine residues, likely due to the availability of additional sites following methanol and low pH-induced unfolding. These data support the conservation of ubiquitin structural elements in the gas phase. The research presented here provides the basis for residue-specific characterization of biomolecules in the gas phase.
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