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Identifying appropriate attachment factors for isolated adult rat cardiomyocyte culture and experimentationLumkwana, Dumisile 04 1900 (has links)
Thesis (MScMedSc)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Introduction: Primary culture of isolated adult rat cardiomyocytes (ARCMs) is an important model for cardiovascular research, but successful maintenance of these cells in culture for their use in experiments remains challenging (Xu et al, 2009; Louch et al, 2011). Most studies are done on acutely isolated cardiomyocytes immediately after isolation, which is due to low survival of these cells in culture. Obstacles in culture are due to the type of medium and attachment factors (tissue culture adhesives) used to culture and grow these cells. Although we previously identified an optimum medium and adhesive for culture, an adhesive that permits cells to remain attached to the culture surface until after an ischemia/reperfusion insult was elusive.
Aims: We therefore aimed to identify the best attachment factor and concentration that will allow adult rat cardiomyocytes to remain attached to the culture surfaces after ischemia/reperfusion experiments. Methods: Cardiomyocytes were isolated from adult Wistar rat hearts and cultured overnight on different concentrations (25 -200 μg/ml) of collagen 1, collagen 4, extracellular matrix (ECM), laminin/entactin (L/E) and laminin. Following overnight cultures, experiments were done in PBS and in PBS versus MMXCB to compare ARCM attachment and viability. Cardiomyocytes cultured on ECM, L/E and L (25−200μg/ml) were subjected to 1 hour of simulated ischemia using MMXCB that contained 3mM SDT and 10mM 2DG, followed by 15 minutes reperfusion. Cell viability was determined by staining cells with JC-1 and images of cells in a field view of 1.17μm/mm2 were captured using fluorescence microscopy. The cells were analysed according to morphology and fluorescence intensity.
Results: Total and rod-shaped ARCMs attachment was improved when MMXCB was used as an experimental buffer instead of PBS. Regardless of the buffer used, morphological viability was poor on substrates of Col 1 and Col 4. In contrast to collagens, ARCMs attached efficiently and morphological viability was high on substrates of ECM, L/E and L in MMXCB, but this was greatly reduced in PBS. Mitochondrial viability was high in MMXCB compared to PBS on Col 1 and Col 4 at 75−175μg/ml and on ECM, L/E and L at all concentrations, except at 50 and 150μg/ml ECM, 175μg/ml L/E and 25μg/ml L.
When cardiomyocytes cultured on ECM, L/E and L were subjected to simulated ischemia, total ARCMs, rod-shaped and R/G fluorescence (mitochondrial viability) was reduced at all concentrations compared to the control group. Hypercontracted cells were higher in the ischemic treated cells compared to the controls on ECM at 75−150μg/ml and 200μg/ml, L/E at 50,100μg/ml and 175μg/ml and on L at 125μg/ml. Total numbers of ARCMs attached on ECM, L/E and L in the ischemic group consisted of similar numbers of non-viable hypercontracted and viable rod-shaped cells.
Conclusion: Cardiomyocytes should be cultured on ECM or L/E or L at concentrations from 25−200μg/ml in MMXCB. PBS is harmful to cultured ARCMs and should thus not be used as an experimental buffer. Ischemia/reperfusion can be simulated on ARCMs cultured on ECM, L/E or L from 25−200μg/ml, provided that a modified culture buffer is used as experimental buffer. / AFRIKAANSE OPSOMMING: Inleiding: Primêre selkulture van geïsoleerde volwasse rot kardiomiosiete (VRKMe) is ‘n belangrike model vir kardiovaskulêre navorsing, maar om hierdie selle suksesvol in kultuur te onderhou is ‘n groot uitdaging (Xu et al, 2009; Louch et al, 2011). Die meeste navorsingstudies maak gebruik van akuut geïsoleerde kardiomiosiete onmiddelik na isolasie omdat oorlewing van hierdie selle in kultuur baie laag is. Die struikelblokke in kultuur is as gevolg van die tipe medium en weefselkultuurgom wat gebruik word. Ons het voorheen 'n optimale medium en weefselkultuurgom geïdentifiseer vir VRKM kultuur oorlewing, maar die weefselkultuurgom was nie effektief genoeg om die selle aan die kultuuroppervlak te laat bly vaskleef, tot na die einde van 'n isgemie/herperfusie eksperiment nie.
Doel: Die doel was dus om die beste weefselkultuurgom en konsentrasie te identifiseer, wat sal toelaat dat VRKMe verbonde bly aan die kultuuroppervlaktes tot na die einde van isgemie/herperfusie eksperimente. Metodes: Kardiomiosiete was geïsoleer vanaf volwasse Wistar rotharte en oornag in kultuur op verskillende konsentrasies (25 -200 μg/ml) van kollageen 1, kollageen 4, ekstrasellulêre matriks (ESM), laminin/entactin (L/E) en laminin onderhou. Die volgende dag was die VRKMe vir eksperimentasie in PBS en in PBS teenoor MMXCB gebruik, om selbehoud en oorlewing te vergelyk. Kardiomiosiete op ESM, L/E en L (25−200μg/ml) was aan 1 uur van gesimuleerde isgemie blootgestel, in MMXCB wat 3mM SDT en 10mM 2DG bevat het, gevolg deur 15 minute herperfusie. Sel oorlewing was bepaal deur selle te kleur met JC-1 en daarna was fluoressensiebeelde van die selle in ‘n veldgebied van 1.17μm/mm2 geneem. Die selle was volgens selmorfologie en fluoressensie intensiteit ontleed.
Resultate: Met die gebruik van MMXCB as eksperimentele buffer in plaas van PBS, het die aantal totale en staafvormige VRKMe verbinding verbeter. Morfologiese onderhoud was sleg op kollageen 1 en 4, ongeag van watter buffer gebruik was. In kontras met die kollagene was die VRKM verbinding en morfologiese onderhoud op ESM, L/E en L in MMXCB effektief verbeter, maar in PBS aansienlik verminder. Mitochondriale lewensvatbaarheid in MMXCB teenoor PBS op kollageen 1 en 4 by 75−175μg/ml, sowel as op ECM, L/E en L by alle konsentrasies, was hoog, behalwe by 50 en 150μg/ml ESM, 175μg/ml L/E en 25μg/ml L.
Isgemie blootstelling van kardiomiosiete gekultuur op alle konsentrasies van ESM, L/E en L, het ‘n afname in die totale, staafvormige en R/G fluoressensie (mitochondriale lewensvatbaarheid) teweeggebring. Meer hiperkontrakteerde kardiomiosiete was in die isgemie behandelde groepe as in die kontrole groepe teenwoordig, spesifiek op ESM by 75−150μg/ml en 200μg/ml, op L/E by 50,100μg/ml en 175μg/ml asook op L by 125μg/ml. In die isgemie groepe het die totale aantal VRKMe op ESM, L/E en L meestal uit ‘n gelyke hoeveelheid hiperkontrakteerde en staafvormige selle bestaan.
Gevolgtrekking: Kardiomiosiete moet op ESM of L/E of L by konsentrasises van 25−200μg/ml in MMXCB gekultuur word. PBS is nadelig vir VRKMe in kultuur en moet dus nie gebruik word as eksperimentele buffer nie. Isgemie/herperfusie eksperimente kan gesimuleer word op VRKMe wat op 25−200μg/ml ESM, L/E of L gekultuur is, mits ‘n gemodifiseerde kultuur buffer gebruik word as eksperimentele buffer.
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The role of Protein Phosphatase 2A (PP2A) in myocardial ischaemia/reperfusion injuryVan Vuuren, Derick 04 1900 (has links)
Thesis (PhD)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Ischaemic heart disease is a major contributor to global morbidity and mortality rates. Manoeuvres
such as ischaemic preconditioning confer cardioprotection against ischaemia/reperfusion (I/R)
injury by activating several intracellular signalling pathways. These pathways have been defined
solely in terms of the kinases involved, despite the realization in recent years that protein
phosphatase activity also contributes significantly to the attributes of the propagated signal. Protein
phosphatase 2A (PP2A) is a heteromultimeric enzyme involved in an array of phosphatase
reactions. We hypothesized that PP2A is an important participant in the myocardial response to I/R
by regulating intracellular signalling.
This project aimed to (i) characterize PP2A during myocardial I/R; (ii) determine the importance of
its contribution to the cellular response to I/R; and (iii) investigate its role in the signalling pathways
mediated by PKB/Akt, GSK-3β, ERK p42/p44 and p38 MAPK.
Two models were used to characterize PP2A during I/R: (i) H9c2 cells exposed to simulated
ischaemia (SI) buffer in conjunction with hypoxia (0.5% O2) for a maximum of 2 hours, followed by
reoxygenation in standard growth medium for up to 30 minutes; and (ii) isolated working rat hearts
exposed to a maximum of 20 minutes global ischaemia and 10 minutes reperfusion. In both
models samples were collected at several time points during I/R for Western blotting analysis.
PP2A-C (the catalytic subunit) accumulated in the nucleus during early ischaemia, but later
redistributed to the cytosol. At the end of ischaemia there was an elevation of PP2A-C relative to
PP2A-A in the unfractionated whole cell preparation concomitant with an increase in the inhibitory
phosphorylation of PP2A-C.
The impact of PP2A activity was evaluated by either inhibiting PP2A using okadaic acid (OA, 10
nM) or activating it by administering FTY720 (1 μM) in an isolated working rat heart model exposed
to either 35 minutes of regional ischaemia (RI) with infarct size (IFS) as primary end-point, or 20
minutes global ischaemia (GI) with functional recovery as end-point. The results showed that the
pre-ischaemic administration of OA or FTY720 reduced or exacerbated IFS respectively, indicating
that PP2A activation during I/R favours cell death. OA and FTY720 were also employed to assess the contribution of PP2A to intracellular signalling
in an isolated working rat heart exposed to I/R. Samples were collected at several timepoints and
analyzed using Western Blotting. Pre-ischaemic administration of OA enhanced the
phosphorylation of PKB/Akt, ERK p42/p44 and GSK-3β at the onset of reperfusion, while FTY720
given before ischaemia reduced the phosphorylation of GSK-3β, p38 MAPK and PKB/Akt at the
end of ischaemia and onset of reperfusion. In summary, PP2A is part of an early nuclear-based response to ischaemia, while long-term
ischaemia induces an increase in PP2A-C. A portion of this PP2A-C is stored in an inactive form,
while an active portion acts as a regulator of the pro-survival signalling components PKB/Akt, GSK-
3β and ERK p42/p44 at the end of ischaemia and the onset of reperfusion. PP2A is therefore an
important component of the myocardial response to I/R by regulating pro-survival signalling. / AFRIKAANSE OPSOMMING: Iskemiese hartsiekte is een van die belangrikste komponente wat bydra tot globale morbiditeit en
mortaliteit. Ingrepe soos iskemiese prekondisionering aktiveer veelvoudige intrasellulêre
seintransduksiepaaie om kardiobeskerming teen iskemie/herperfusie (I/H)-besering te ontlok. Die
kinases betrokke in hierdie seintransduksiepaaie is reeds deeglik nagevors, terwyl die potensiële
belang van die proteïenfosfatases in seintransduksie tot onlangs misken is. Ons hipotese was dat
Proteïenfosfatase 2A (PP2A), wat in ‘n wye verskeidenheid fosfatase reaksies betrokke is, ‘n
belangrike rolspeler in die miokardiale reaksie op I/H-besering is, deur deelname aan die
regulering van intrasellulêre seintransduksie.
Hierdie projek het ten doel gehad om (i) PP2A te karakteriseer tydens miokardiale I/H; (ii) die
belang van PP2A in die sellulêre reaksie op I/H-besering te bepaal; en (iii) PP2A se rol in die
seintransduksiepaaie, gemedieer deur PKB/Akt, GSK-3β, ERK p42/p44 en p38 MAPK, te
evalueer.
Twee modelle is aangewend om PP2A tydens I/H te karakteriseer: (i) H9c2-selle blootgestel aan ‘n
simuleerde iskemiebuffer tesame met hipoksie (0.5% O2) vir ‘n maksimum van 2 uur gevolg deur
heroksiginasie in standaardgroeimedium vir verskillende tydsperiodes tot ‘n maksimum van 30
minute; en (ii) geïsoleerde, werkende rotharte blootgestel aan ‘n maksimum van 20 minute globale
iskemie en 10 minute herperfusie. In beide modelle is monsters op verskillende tye versamel vir
Western-kladanalise. Tydens vroeë iskemie het PP2A-C in die kern toegeneem, waarna dit met
verloop van tyd na die sitosol herversprei het. Teen die einde van iskemie was daar ‘n toename in
die vlakke van PP2A-C relatief tot PP2A-A in ongefraksioneerde weefselhomogenate, tesame met
‘n toename in die inhibitoriese fosforilering van PP2A-C. Die belang van PP2A-aktiwiteit is ondersoek deur die effek te bepaal van die inhibisie of aktivering
daarvan op infarktgrootte (IFS) en funksionele herstel in ‘n geïsoleerde werkende rothartmodel,
blootgestel aan onderskeidelik 35 minute streeksiskemie (RI) of 20 minute globale iskemie. Preiskemiese
toediening van die PP2A-inhibitor okadaïensuur (OA, 10 nM), of aktiveerder FTY720 (1
μm) het infarktgrootte respektiewelik beperk of vergroot. PP2A-aktivering tydens I/H is dus nadelig.
OA en FTY720 is ook aangewend om die bydrae van PP2A tot I/H-verwante, intrasellulêre
seintransduksie in die geïsoleerde, werkende rothart te bepaal. Monsters is op verskeie
tydintervalle versamel en ontleed deur gebruik te maak van die Western-kladtegniek. Preiskemiese
toediening van OA het die fosforilering van PKB/Akt, ERK p42/p44 en GSK-3β by die
aanvang van herperfusie bevoordeel, terwyl pre-iskemiese toediening van FTY720, die fosforilering van GSK-3β, p38 MAPK en PKB/Akt aan die einde van iskemie en die begin van
herperfusie verminder het.
Ter opsomming: PP2A is deel van ‘n vroeë gelokaliseerde kerngebaseerde reaksie op iskemie,
terwyl langdurige iskemie ‘n toename in PP2A-C relatief tot PP2A-A induseer. ‘n Deel van hierdie
PP2A-C is onaktief, terwyl die res funksioneer in die regulering van die
seintransduksiekomponente PKB/Akt, GSK-3β en ERK p42/p44 wat oorlewing fasiliteer met die
aanvang van herperfusie. PP2A is dus ‘n belangrike komponent in die miokardiale reaksie op I/H
deurdat dit tot die beheer van seintransduksiepaaie bydra.
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Etude de la réactivité et de la toxicité des particules de méthoxyphénols : analyse de leur action in vivo chez le rat en atmosphère contrôlée sur la fonction cardiaque et les paramètres du stress oxydant. / Study of the reactivity and toxicity of methoxyphenols particle : analyse of their action in vivo in the rat in a controlled atmosphere on cardiac function and oxidative stress parameters.Ricquebourg, Emilie 15 April 2014 (has links)
De manière générale, l'inhalation de particules entraîne des réactions inflammatoires et des réactions d'oxydo-réduction responsables de la dégradation des matrices biologiques qui exercent, de plus, un fort impact cardio-vasculaire. La combustion du bois est une source majeure de composés organiques semi-volatils, parmi lesquels les méthoxyphénols (MPs), tels que le coniféryl aldéhyde (CA), le syringaldéhyde (SR), ou l'acétosyringone (AS). Les MPs sont néanmoins peu étudiés dans la littérature alors que la toxicité d'autres composés également issus de la combustion de la biomasse, tels que le monoxyde de carbone, les suies et les hydrocarbures polyaromatiques est intensivement étudiée. Ce travail a montré par GC/MS que le vieillissement en atmosphère simulée (ozone, rayonnements lumineux) dégrade le CA en produits secondaires moins cytotoxiques, évalués sur des fibroblastes en culture, mais préserve le taux atmosphérique de SR et AS, de toxicité avérée. Un dispositif original de production de MPs particulaires (Ø~50nm, N~7E4particules/cm3, m~5µg/m3) en atmosphère contrôlée a été validé et permet la 1ièreétude in vivo des MPs. L'exposition chez le rat (1-3mois) montre une modification des défenses antioxydantes et des changements cardiaques principalement avec AS, puis CA et un peu moins pour SR. Des processus adaptatifs sont démontrés après 5mois d'exposition.Par ailleurs, il a été montré in vitro sur des adénocacinomes pulmonaires A549 en culture, que le CA induit une destructuration du tapis cellulaire et l'apoptose (caspase 3) mais pas d'effet pro-inflammatoire (IL8, Cox-2). En conclusion, ce travail contribue à étudier l'impact des MPs in vitro et in vivo. / In general, inhalation of particles is at the origin of inflammatory and oxidative reactions who are responsible of the degradation of biological cellular constituents, and could have a strong cardiovascular impact. The wood combustion is a major source of semivolatile organic compounds such as the methoxyphenols (MPs) including coniferyl aldehyde (CA), syringaldehyde (SR), or acetosyringone (AS). The MPs are however few studies into literature while toxicity of other compounds also from biomass combustion, as carbon monoxide, soot and polycyclic aromatic hydrocarbon are intensively studies.This work has shown by GC/MS that aging in simulated atmosphere (ozone + light rays) degraded CA in secondary products less cytotoxic, studies on fribroblastes culture but keep the atmospherical level of SR and AS which have a toxicity proved.A device of MPs particle production original by atomization, with a check system (height, composition, weight) and exposition flow continuous (Ø~50 nm, N~7E4 particles/cm3, m~5 µg/m3) adapted to little animals, was developed and validated, allowed the first study in vivo with these molecules. Between 1 and 3 month of exposition to rat Wistar, show modified antioxidant defences and cardiac modification (ischaemia/reperfusion) principally with AS, then CA and less SR. The adaptatives processes (remodeling) are demonstrated after 5 month of exposition.Furthermore, it is showed in vitro on lung adenocacinum cell lines (A549), CA induced a monolayer destructuration and apoptosis (caspase 3) but no effect proinflammatory (IL8, Cox-2 and iNOS).To conclude, this work contributes to study the impact of MPs in vitro and in vivo.
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