submitted by Liu Dan. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 66-80). / Abstract also in Chinese. / CONTENTS --- p.i / LIST OF TABLES --- p.iii / LIST OF FIGURES --- p.iv / LIST OF ABBREVIATIONS --- p.v / ACKNOWLEDGEMENTS --- p.vi / ABSTRACT --- p.vii / Chapter Chapter 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- History --- p.1 / Chapter 1.2 --- Immunoglobulins --- p.3 / Chapter 1.2.1 --- Structure --- p.3 / Chapter 1.2.2 --- Properties of immunoglobulins --- p.7 / Chapter 1.3 --- Monoclonal proteins and monoclonal gammopathies --- p.12 / Chapter 1.3.1 --- Monoclonal proteins --- p.12 / Chapter 1.3.2 --- Monoclonal gammopathies --- p.14 / Chapter 1.4 --- Laboratory investigation of monoclonal immunoglobulin --- p.17 / Chapter 1.4.1 --- The current procedure of investigation in laboratory --- p.17 / Chapter 1.4.2 --- Problems in identifying monoclonal immunolgobuin --- p.19 / Chapter 1.5 --- Comparison of different techniques --- p.20 / Chapter 1.5.1 --- Immunoelectrophoresis --- p.20 / Chapter 1.5.2 --- Immunofixation electrophoresis --- p.22 / Chapter 1.5.3 --- Isoelectric focusing and immunoisoelectric focusing --- p.24 / Chapter 1.6 --- Aim of the present study --- p.27 / Chapter 1.7 --- Design of experiment --- p.27 / Chapter Chapter 2 --- MATERIALS AND METHODS --- p.30 / Chapter 2.1 --- Study subjects --- p.30 / Chapter 2.2 --- Apparatus --- p.30 / Chapter 2.2 --- Apparatus --- p.30 / Chapter 2.3 --- Reagents and materials --- p.32 / Chapter 2.4 --- Preparation of gels --- p.35 / Chapter 2.5 --- Isoelectric focusing procedure --- p.36 / Chapter 2.6 --- Acid fixation and staining --- p.37 / Chapter 2.7 --- Technical factors affecting results --- p.38 / Chapter Chapter 3 --- RESULTS --- p.40 / Chapter 3.1 --- Interpretation of results in isoelectric focusing --- p.40 / Chapter 3.2 --- Affecting factors --- p.47 / Chapter 3.3 --- Comparison of the results between IEF and IFE --- p.53 / Chapter Chapter 4 --- DISCUSSION --- p.59 / Chapter Chapter 5 --- CONCLUSION --- p.65 / References --- p.66

O entendimento da contribuição diferencial de processos neutros e adaptativos envolvidos na diferenciação genética entre populações, assim como sua relação com varáveis físicas e ambientais da área de distribuição das espécies, é fundamental para melhorar o conhecimento da história evolutiva, mas também para fazer um manejo e conservação mais adequados da diversidade genética das espécies. O surgimento da Planície Costeira do Atlântico Sul foi um processo relativamente recente, que conduziu a processos de colonização e expansão dos organismos para um ambiente costeiro. Os padrões de estrutura genética gerados em processos de colonização e expansão podem ser difíceis de interpretar devido ao fato de que podem apresentar sinais sobrepostos de efeito fundador em série, isolamento por distância e isolamento por ambiente quando envolvem gradientes ecológicos na área de estudo. No presente trabalho foram conduzidas caracterizações da diversidade e estrutura genética de dois taxa predominantemente costeiros co-distribuídos, Calibrachoa heterophylla e Petunia integrifolia ssp. depauperata, em toda a amplitude da distribuição. Também foram inferidas as dinâmicas de fluxo gênico entre populações e sua relação com variáveis topográficas e climáticas reconstruídas pelo meio de um levantamento exaustivo e modelamento para a área de estudo. Processos de diferenciação genética promovidos pelo regime diferencial de chuvas nos extremos da distribuição foram inferidos para as duas espécies. Também foram identificadas populações das duas espécies apresentando alto nível de mistura de identidade genética nas localidades ao redor da Lagoa dos Patos. Isso foi associado a alta instabilidade na história geomorfológica recente desta região e dinâmicas atuais do vento que favorecem a dispersão secundária de sementes a maiores distâncias. Adicionalmente foram identificados processos espécie-específicos que se relacionaram principalmente a fatores históricos de cada táxon. Em P. depauperata o efeito fundador relacionado a um processo único de colonização do ambiente costeiro determinou o nível superior de estrutura genética, enquanto que em C. heterophylla foi a história filogeográfica da espécie na qual a diferenciação intraespecífica é anterior à colonização da região costeira atual o fator preponderante. As diferenças de duração do ciclo de vida entre as espécies também influenciaram as dinâmicas contrastantes de fluxo gênico dos dois taxa, sugerindo que a colonização e adaptação local de C. heterophylla nas bordas da distribuição poderia ser condizente com um processo de monopolização. Em vista dos resultados obtidos neste trabalho, propõem-se o desenvolvimento de experimentos de transplante recíproco para confirmar o processo de adaptação local nas duas espécies e abordagens genômicas para identificar regiões do genoma responsáveis pelos processos de adaptação ao ambiente costeiro e de adaptação local nas margens da distribuição. / The understanding of differential contribution of neutral and adaptive processes to the genetic differentiation among populations, as well as its relationship to physical and environmental variables of species’ distribution area, is essential to improve the knowledge of species evolutionary history, but also to direct appropriate management and conservation policies for the genetic diversity. The emergence of the South Atlantic Coastal Plain was a relatively recent event that led to colonization and expansion processes to the coastal environment. Genetic structure patterns generated in colonization and expansion processes can be difficult to interpret because the overlapping signals, which can present the founder effect in series, isolation by distance, and isolation by environment in the presence of ecological gradients in the study area. In this work characterization diversity and genetic structure were conducted to two co-distributed and predominantly coastal taxa, Calibrachoa heterophylla and Petunia integrifolia ssp. depauperata alongside their complete geographical range. Moreover, we also inferred dynamic of gene flow among populations and investigated the relation between topographical and climatic variables reconstructed by means of an exhaustive survey and modeling for the study area and the gene flow. Shared genetic differentiation processes promoted by differential rainfall conditions at the distribution edges were inferred. In addition, we identified populations from both species with high level of mixed genetic membership in locations around the Patos Lagoon. This was associated with a high instability in recent geomorphological history of coastal region and current wind dynamics that favor the secondary seed dispersal over longer distances. Additionally, specific species processes were identified mainly related to historical factors of each taxon. In P. depauperata founder effects associated with unique colonization process to coastal environment determined the upper level of genetic structure, while in C. heterophylla the upper level of genetic structure was related to the phylogeographical history wherein the intra-specific differentiation preceded colonization to the current coastal region. The differences of the life span length of the species were also related to contrasting gene flow dynamics indicating that the colonization and local adaptation of C. heterophylla at the edges of the distribution could lead to monopolization process. In view of the results we propose the development of reciprocal transplant experiments to confirm the local adaptation process in both species and genomic approaches to identify regions of the genome responsible for the processes of adaptation to the coastal environment and local adaptation in distribution margins.

O entendimento da contribuição diferencial de processos neutros e adaptativos envolvidos na diferenciação genética entre populações, assim como sua relação com varáveis físicas e ambientais da área de distribuição das espécies, é fundamental para melhorar o conhecimento da história evolutiva, mas também para fazer um manejo e conservação mais adequados da diversidade genética das espécies. O surgimento da Planície Costeira do Atlântico Sul foi um processo relativamente recente, que conduziu a processos de colonização e expansão dos organismos para um ambiente costeiro. Os padrões de estrutura genética gerados em processos de colonização e expansão podem ser difíceis de interpretar devido ao fato de que podem apresentar sinais sobrepostos de efeito fundador em série, isolamento por distância e isolamento por ambiente quando envolvem gradientes ecológicos na área de estudo. No presente trabalho foram conduzidas caracterizações da diversidade e estrutura genética de dois taxa predominantemente costeiros co-distribuídos, Calibrachoa heterophylla e Petunia integrifolia ssp. depauperata, em toda a amplitude da distribuição. Também foram inferidas as dinâmicas de fluxo gênico entre populações e sua relação com variáveis topográficas e climáticas reconstruídas pelo meio de um levantamento exaustivo e modelamento para a área de estudo. Processos de diferenciação genética promovidos pelo regime diferencial de chuvas nos extremos da distribuição foram inferidos para as duas espécies. Também foram identificadas populações das duas espécies apresentando alto nível de mistura de identidade genética nas localidades ao redor da Lagoa dos Patos. Isso foi associado a alta instabilidade na história geomorfológica recente desta região e dinâmicas atuais do vento que favorecem a dispersão secundária de sementes a maiores distâncias. Adicionalmente foram identificados processos espécie-específicos que se relacionaram principalmente a fatores históricos de cada táxon. Em P. depauperata o efeito fundador relacionado a um processo único de colonização do ambiente costeiro determinou o nível superior de estrutura genética, enquanto que em C. heterophylla foi a história filogeográfica da espécie na qual a diferenciação intraespecífica é anterior à colonização da região costeira atual o fator preponderante. As diferenças de duração do ciclo de vida entre as espécies também influenciaram as dinâmicas contrastantes de fluxo gênico dos dois taxa, sugerindo que a colonização e adaptação local de C. heterophylla nas bordas da distribuição poderia ser condizente com um processo de monopolização. Em vista dos resultados obtidos neste trabalho, propõem-se o desenvolvimento de experimentos de transplante recíproco para confirmar o processo de adaptação local nas duas espécies e abordagens genômicas para identificar regiões do genoma responsáveis pelos processos de adaptação ao ambiente costeiro e de adaptação local nas margens da distribuição. / The understanding of differential contribution of neutral and adaptive processes to the genetic differentiation among populations, as well as its relationship to physical and environmental variables of species’ distribution area, is essential to improve the knowledge of species evolutionary history, but also to direct appropriate management and conservation policies for the genetic diversity. The emergence of the South Atlantic Coastal Plain was a relatively recent event that led to colonization and expansion processes to the coastal environment. Genetic structure patterns generated in colonization and expansion processes can be difficult to interpret because the overlapping signals, which can present the founder effect in series, isolation by distance, and isolation by environment in the presence of ecological gradients in the study area. In this work characterization diversity and genetic structure were conducted to two co-distributed and predominantly coastal taxa, Calibrachoa heterophylla and Petunia integrifolia ssp. depauperata alongside their complete geographical range. Moreover, we also inferred dynamic of gene flow among populations and investigated the relation between topographical and climatic variables reconstructed by means of an exhaustive survey and modeling for the study area and the gene flow. Shared genetic differentiation processes promoted by differential rainfall conditions at the distribution edges were inferred. In addition, we identified populations from both species with high level of mixed genetic membership in locations around the Patos Lagoon. This was associated with a high instability in recent geomorphological history of coastal region and current wind dynamics that favor the secondary seed dispersal over longer distances. Additionally, specific species processes were identified mainly related to historical factors of each taxon. In P. depauperata founder effects associated with unique colonization process to coastal environment determined the upper level of genetic structure, while in C. heterophylla the upper level of genetic structure was related to the phylogeographical history wherein the intra-specific differentiation preceded colonization to the current coastal region. The differences of the life span length of the species were also related to contrasting gene flow dynamics indicating that the colonization and local adaptation of C. heterophylla at the edges of the distribution could lead to monopolization process. In view of the results we propose the development of reciprocal transplant experiments to confirm the local adaptation process in both species and genomic approaches to identify regions of the genome responsible for the processes of adaptation to the coastal environment and local adaptation in distribution margins.

O entendimento da contribuição diferencial de processos neutros e adaptativos envolvidos na diferenciação genética entre populações, assim como sua relação com varáveis físicas e ambientais da área de distribuição das espécies, é fundamental para melhorar o conhecimento da história evolutiva, mas também para fazer um manejo e conservação mais adequados da diversidade genética das espécies. O surgimento da Planície Costeira do Atlântico Sul foi um processo relativamente recente, que conduziu a processos de colonização e expansão dos organismos para um ambiente costeiro. Os padrões de estrutura genética gerados em processos de colonização e expansão podem ser difíceis de interpretar devido ao fato de que podem apresentar sinais sobrepostos de efeito fundador em série, isolamento por distância e isolamento por ambiente quando envolvem gradientes ecológicos na área de estudo. No presente trabalho foram conduzidas caracterizações da diversidade e estrutura genética de dois taxa predominantemente costeiros co-distribuídos, Calibrachoa heterophylla e Petunia integrifolia ssp. depauperata, em toda a amplitude da distribuição. Também foram inferidas as dinâmicas de fluxo gênico entre populações e sua relação com variáveis topográficas e climáticas reconstruídas pelo meio de um levantamento exaustivo e modelamento para a área de estudo. Processos de diferenciação genética promovidos pelo regime diferencial de chuvas nos extremos da distribuição foram inferidos para as duas espécies. Também foram identificadas populações das duas espécies apresentando alto nível de mistura de identidade genética nas localidades ao redor da Lagoa dos Patos. Isso foi associado a alta instabilidade na história geomorfológica recente desta região e dinâmicas atuais do vento que favorecem a dispersão secundária de sementes a maiores distâncias. Adicionalmente foram identificados processos espécie-específicos que se relacionaram principalmente a fatores históricos de cada táxon. Em P. depauperata o efeito fundador relacionado a um processo único de colonização do ambiente costeiro determinou o nível superior de estrutura genética, enquanto que em C. heterophylla foi a história filogeográfica da espécie na qual a diferenciação intraespecífica é anterior à colonização da região costeira atual o fator preponderante. As diferenças de duração do ciclo de vida entre as espécies também influenciaram as dinâmicas contrastantes de fluxo gênico dos dois taxa, sugerindo que a colonização e adaptação local de C. heterophylla nas bordas da distribuição poderia ser condizente com um processo de monopolização. Em vista dos resultados obtidos neste trabalho, propõem-se o desenvolvimento de experimentos de transplante recíproco para confirmar o processo de adaptação local nas duas espécies e abordagens genômicas para identificar regiões do genoma responsáveis pelos processos de adaptação ao ambiente costeiro e de adaptação local nas margens da distribuição. / The understanding of differential contribution of neutral and adaptive processes to the genetic differentiation among populations, as well as its relationship to physical and environmental variables of species’ distribution area, is essential to improve the knowledge of species evolutionary history, but also to direct appropriate management and conservation policies for the genetic diversity. The emergence of the South Atlantic Coastal Plain was a relatively recent event that led to colonization and expansion processes to the coastal environment. Genetic structure patterns generated in colonization and expansion processes can be difficult to interpret because the overlapping signals, which can present the founder effect in series, isolation by distance, and isolation by environment in the presence of ecological gradients in the study area. In this work characterization diversity and genetic structure were conducted to two co-distributed and predominantly coastal taxa, Calibrachoa heterophylla and Petunia integrifolia ssp. depauperata alongside their complete geographical range. Moreover, we also inferred dynamic of gene flow among populations and investigated the relation between topographical and climatic variables reconstructed by means of an exhaustive survey and modeling for the study area and the gene flow. Shared genetic differentiation processes promoted by differential rainfall conditions at the distribution edges were inferred. In addition, we identified populations from both species with high level of mixed genetic membership in locations around the Patos Lagoon. This was associated with a high instability in recent geomorphological history of coastal region and current wind dynamics that favor the secondary seed dispersal over longer distances. Additionally, specific species processes were identified mainly related to historical factors of each taxon. In P. depauperata founder effects associated with unique colonization process to coastal environment determined the upper level of genetic structure, while in C. heterophylla the upper level of genetic structure was related to the phylogeographical history wherein the intra-specific differentiation preceded colonization to the current coastal region. The differences of the life span length of the species were also related to contrasting gene flow dynamics indicating that the colonization and local adaptation of C. heterophylla at the edges of the distribution could lead to monopolization process. In view of the results we propose the development of reciprocal transplant experiments to confirm the local adaptation process in both species and genomic approaches to identify regions of the genome responsible for the processes of adaptation to the coastal environment and local adaptation in distribution margins.

No description available.

Call number: LD2668 .T4 1984 W53 / Master of Science

Master of Science / Department of Architectural Engineering and Construction Science / Sutton F. Stephens / Seismic hazards are a primary concern in some of the most populous regions in the United States. Performance-based seismic design has brought about new technology advances and introduced an innovative approach towards constructing seismic resistant buildings. Base isolation and structural damping systems are becoming increasingly utilized methods of advanced seismic resistance. This relatively new design approach presents various issues that must be addressed throughout the design and construction processes. A brief background on the origin, dynamics, and hazards of earthquakes and a discussion on designs of traditional, fixedbased structures is presented in this report. A description for selected types of new advanced seismic restraint systems, with an emphasis on base isolation, is also provided. Examples of current applications of buildings equipped with base isolation are presented. This report concludes with a review of the fundamental design methodology for structural base isolation along with additional requirements not addressed by the current building codes.

Text in German / Zusammenfassung Die vorliegende Untersuchung geht von der Beobachtung aus, dass die postmoderne Gesellschaft tendenziell isolierend auf die Menschen wirkt. Demgegenüber wohnt der Kirche ein Gemeinschaftspotential inne, das eine greifbare Alternative für vereinsamte Menschen anbieten kann. Diese Untersuchung stellt nun die zentrale Frage, wie die Kirche ihr gemeinschaftsförderndes Potential effektiver in die Gesellschaft einbringen kann. Hierzu werden zunächst die soziologischen und theologischen Voraussetzungen geklärt, denen dann, anhand einer begrenzten empirischen Studie, praktische Erfahrungen von Menschen mit kirchlicher Gemeinschaft an die Seite gestellt werden. Zuletzt wird die zeitgenössische Gemeindebauliteratur vergleichend herangezogen, um schlussendlich die zentrale Frage dieser Untersuchung mit einigen praktischen Vorgehensvorschlägen zu beantworten. Summary of Dissertation This study has its roots in the observation, that the postmodern society has a tendency to isolate the people. In contrast to this, the church has an inherent potential of community, which can offer a concrete alternative for isolated people. This study now asks the central question, how the church can be enabled to bring their community-promoting potential more effectively into the society. For this, firstly the sociological and theological conditions are clarified, to which then, on the basis of a limited empirical study, practical experiences of people with church community are placed beside. At last, the contemporary literature of church growth is consulted comparatively, in order to finally answer the central question of this study by some practical procedure suggestions. / Practical Theology / M.Th. (Practical Theology)

Les mousses actives sont des solutions composites de contrôle du bruit qui combinent les avantages complémentaires du matériau en mousse passif et des actionneurs piézoélectriques répartis spatialement à l'intérieur des mousses. Une étude sur le problème de l'amélioration de l'indice d'affaiblissement des mousses actives en utilisant des stratégies de contrôle actif a été effectuée à la fois numériquement et expérimentalement à l'intérieur d'un guide d'onde sous la condition de propagation en ondes planes. Trois différents modèles de prototypes de mousse active ont été pris en compte dans les simulations par éléments finis et leur efficacité à annuler l'onde transmise en aval de la mousse active a été étudiée. Des études expérimentales afin d'optimiser l'indice d'affaiblissement des mousses actives sous une commande prédictive SISO avec un microphone unidirectionnel comme capteur d'erreur démontrent que l'efficacité du contrôle sur une large gamme de fréquences est bonne. Le problème physique de l'annulation de la propagation des ondes sonores est étudié en détail dans les simulations numériques et elles apportent un éclairage précieux sur l'altération de la réponse vibratoire de l'actionneur piézo-électrique de la mousse active sous contrôle optimal.Les résultats des simulations ont aussi contribué à l'identification de stratégies de contrôle-de rechange pour l'atténuation de l'onde sonore transmise à l'aide de la réponse sensorielle de l'actionneur distribué. On peut pour cela remplacer éventuellement l'utilisation de microphones en champ lointain et ainsi améliorer notablement la compacité du système de contrôle actif. La réponse sensorielle d'un piezo-actionneur, en raison de sa déformation mécanique est indépendante de la réponse de sa charge totale, avec la compensation analogique-numérique hybride de la"capacite feedthrough" de l'actionneur, à l'aide d'un algorithme adaptatif. Cette charge mécanique de réponse s'est revélé être une bonne approximation de la vitesse radiale du volume de l'actionneur, et peut être utilisée comme signal d'erreur pour maximiser l'indice d'affaiblissement du système de mousse active. En outre, elle a été utilisée dans l'absorption et les problèmes de contrôle TL, fonctionnant sur une erreur de stratégie virtuelle de détection, et a produit les résultats souhaités sur une large plage de fréquences. Le succès du principe capteur/actionneur dans les problèmes de contrôle actif du bruit peut donner des améliorations importantes en termes de positions et de configurations de capteurs d'erreurs associés aux systèmes de contrôle actif.

As a technique to ensure absence of undesired interference in transactional computations, Concurrency Control (CC) guarantees logical data consistency via providing transaction isolation, thus contributing to their dependability. However, single-version CC, which requires that a transaction system always works on the current version of a data item, may introduce unpredictable delays for real-time transactions because of unbounded blocking time which may cause deadline misses. Compared to single-version CC (current value of a data item is available but the historical values are overwritten and not accessible) mechanism, multi-version Concurrency Control (MVCC, historical values of a data item are maintained in a version list and accessible) mechanisms have several advantages. The benefit of multiple versions for concurrency control is helping the scheduler avoid rejecting operations, which could improve the concurrency for real-time transaction systems. Because transactions are less likely to be blocked using MVCC, timeliness could be improved. Transaction isolation levels, out of which the serializable one is the highest, control the degree of interference-freedom of concurrent transactions. Instead of serializable isolation, some MVCC mechanisms are known to achieve a relaxed level of isolation. In order to select an appropriate MVCC mechanism that guarantees both timeliness and an acceptable level of isolation for a given transaction set, trade-off analysis between isolation and timeliness is necessary. However, even though approaches have been proposed to analyze timeliness and isolation together, they only focus on lock-based single-version concurrency control algorithms, not on MVCC. In this thesis, we focus on modeling multi-version based real-time transaction system as a network of timed automata, and verify the consistency of the tradeoff transaction timeliness and isolation in UPPAAL. We propose a modular modeling approach to model real-time multi-version transaction systems by reusing and extending set of basic blocks. The proposed approach not only reduces the modeling efforts, but also enables easy adjustment for adapting current MVCC mechanism to another. Assuming a given transaction set, we model three MVCC algorithms including multi-version Timestamp Ordering, a variant of multi-version Two-Phase locking and a Two-Version Priority Ceiling Protocol, and verify both timeliness and isolation level. The verification results show that Two-Version Priority Ceiling Protocol outperforms the other two MVCC algorithms with the given transaction set.