Summary: The possible involvement of an adrenergic-endorphin system in the mediation of the pressor response to isometric muscular contraction was studied in cats. Fatiguing contractions of the gastrocnemius and plantaris muscles caused an increase in the mean arterial blood pressure by 35 to 70 mmHg. Intravenous infusion (30 μg·kg-1) as well as intracisternal injection (2.5 μg) of clonidine-HCl eliminated the pressor response to muscular contraction. In both sets of experiments, the mean blood pressure remained at the resting level throughout the duration of the isometric contraction. Injection of naloxone (0.5 μmol·litre-1) into the cisterna magna did not alter the resting blood pressure and did not affect the rise in mean arterial pressure during muscle contractions. Intracisternal injection of naloxone (0.5 μmol·litre-1) prior to an intracisternal injection of clonidine (2.5 μg) did not alter the resting blood pressure but effectively antagonised the anti-pressor effects of clonidine during fatiguing isometric contractions. These data may indicate that activation of muscle "ergoreceptor" afferents (group III and IV fibres) during muscular contraction may cause an increase in the arterial blood pressure by interfering with an inhibitory adrenergic-endorphinergic pathway in the brainstem.

Many clinicians believe exercise associated muscle cramps (EAMC) occur because of dehydration and electrolyte losses. Experimental research supporting this theory is lacking. Mild hypohydration (3% body mass loss) does not alter cramp threshold frequency (TF), a measure of cramp susceptibility, when fatigue and exercise intensity are controlled. No experimental research has examined TF following significant (3-5% body mass loss) or serious hypohydration (>5% body mass loss). TF and blood variables of ten subjects were measured pre-post exercise. Subjects exercised in an environmental heat chamber alternating between upper arm ergometry and non-dominant leg cycling every 15 minutes until they lost 5% of their body mass or were too exhausted to continue. Significant (n=5) or serious hypohydration (n=5) did not alter cramp TF, cramp intensity, or cramp EMG amplitude. Significant and serious hypohydration with moderate electrolyte losses does not alter cramp susceptibility when fatigue and exercise intensity are controlled.

Summary: The effects of clonidine, a central alpha2 agonist, on changes in blood pressure caused by muscle afferent nerve (ergoreceptor) activation and baroreceptor manipulation were studied in cats. Prolonged isometric contractions (ergoreceptor activation) of the gastrocnemius and plantaris muscles increased mean arterial pressure by 53 mmHg. This pressor response was not altered by naloxone (0.5 μmol·litre-1) but was eliminated by clonidine (0.5-2.0 μg) when injected into the cerebral aqueduct. Brief occlusion of the carotid artery (15-30 s) caused mean arterial pressure to increase by 32-42 mmHg at rest. Neither naloxone nor clonidine altered the magnitude of the reflex pressor response to carotid occlusion. Similar increases in pressure were measured when occlusion was applied during fatiguing isometric contractions; thus baroreceptor induced increases in pressure were superimposed on the ergoreceptor induced blood pressure changes. Naloxone did not affect the changes in pressure caused by either reflex response. Clonidine continued to eliminate the pressor response to muscular contraction but did not affect the pressure increase when the carotid occlusion was applied during contractions. Electrical stimulation of the carotid sinus nerve caused blood pressure to decrease by 36 mmHg during rest and by 41 mmHg during fatiguing isometric contractions. Clonidine did not alter the depressor response to carotid sinus nerve stimulation. These data may indicate that separate pathways centrally mediate the changes in blood pressure caused by ergoreceptor and baroreceptor afferent activation. The integration of the ergoreceptor pathway may involve a catecholaminergic-opioidergic system but the present results do not suggest a similar interaction for the baroreceptor integration.

<p> The interrelationship of force, surface elecrograms (EMG) and joint position for static voluntary contractions of Quadriceps Femoris muscle group in man were investigated before and during partial curarization induced by d-tubocurarine. </p> <p> Four normal male volunteers were studied. Each performed a series of brief isometric contractions (by extension of the lower leg against resistance) at different levels of force and at three knee-joint positions while lying in the supine position. All series were repeated for both a normal state and a partially paralyzed state under the :influence of curare. Torque generated about the knee-joint was measured with a Cybex isokinetic system and the nyoelectric activity of three quadriceps muscles was monitored using bipolar surface electrodes. </p> <p> Traditional parameters of nyoelectric activity (mean-rectified-EMG [MRE], and root-mean-squared-DG [RMSE]) were calculated using a minicomputer (PDPll/34), which had also acquired and processed the data. In addition, EMG power spectra were computed by Fast Fourier Transform techniques in an attempt to provide further insight into the effects of curare on human muscle. </p> <p> In order to provide a basis for comparison of the normal state with the parially curarized state, force-EMG relationships were computed for each subject, muscle, knee-joint angle, and condition. Statistical methods (three-way ANOVA' s) were then employed to both quantify any differences that may have existed between the two states and to identify sources of differences within each state. A similar statistically- based comparison of the power spectra was undertaken utilizing several indices that described the shape of the spectra. A general description of the activities of the quadriceps femoris muscles followed after collating all the information that the surface DIG provided in conjunction with the external forces measured. </p> <p> It was concluded that curare did not have any significant effects on the force-EMG relationship. There appeared to be a slight effect of curare on the power spectra however, with a general trend of increasing lower frequency power. The greatest source of variation of force-EMG relationships and power spectra was attributed to the position of the knee-joint. </p> / Thesis / Master of Engineering (MEngr)

A Síndrome Dolorosa Femoropatelar (SDFP), possui etiologia multifatorial e acomete cerca de 7 a 15% da população, em sua maioria mulheres, jovens, adultas e ativas. Ela causa dor anterior ou retropatelar e é exacerbada durante gestos motores funcionais, tais como subir e descer escadas ou permanecer longos períodos de tempo sentado, agachado ou ajoelhado. Como a avaliação diagnóstica desta síndrome ainda é indireta, diversos mecanismos e metodologias buscam realizar uma classificação que diferencie os portadores de SDFP com relação aos assintomáticos. Deste modo, o objetivo desse trabalho é estudar as relações entre os sinais eletromiográficos (EMG) do músculo quadríceps em indivíduos com SDFP durante exercícios isométricos distintos (dissipativo e conservativo) em diferentes ângulos da articulação tíbiofemoral, e compará-los com os indivíduos sem SDFP. Foram analisadas a intensidade do sinal EMG (RMS), a frequência mediana (Fmed) e a frequência em 95% (F95) do espectro de potência dos músculos vasto medial oblíquo (VMO), vasto lateral (VL) e reto femoral (RF) para as contrações isométricas dissipativa e conservativa. Participaram deste estudo 24 voluntários do sexo feminino (17 assintomáticos e 7 com SDFP) que realizaram 18 contrações nos ângulos de 90º, 60º e 20º de extensão de joelho, utilizando 30% da carga voluntária máxima. Eletrodos EMG foram posicionados nos referidos músculos para a coleta dos dados, os quais foram posteriormente tratados e processados, possibilitando a análises do sinal EMG nos domínio do tempo e da frequência. Os resultados indicam que o RMS do sinal EMG apresenta-se distinto entre as contrações isométricas para o grupo sintomático e evidenciam que não existe uma relação de intensidade de contração EMG de um músculo comparativamente ao outro quando se altera o gesto motor. A Fmed de VMO e VL podem ser utilizadas como ferramenta na detecção de SDFP, visto que atuam de maneira distinta entre os grupos. A F95 do músculo VMO comporta-se de um modo diferente entre os grupos, sendo que durante a contração isométrica dissipativa em 20º e 60º, seus valores em SDFP foram maiores do que no grupo Controle. Desta maneira, foi possível relatar que é possível diferenciar a SDFP, ou até mesmo detectá-la, utilizando parâmetros EMG de padrões diferentes de contrações isométricas. / The Patellofemoral Pain Syndrome (PFPS) has a multifactorial etiology and affects approximately 7-15% of the population, mostly active young women. PFPS causes retropatellar pain, that is exacerbated during motor functional gestures, such as climbing stairs, sitting, squatting or kneeling for long periods of time. Since the diagnostic evaluation of this syndrome is still indirect, different mechanisms and methodologies seek to achieve a classification able to distinguish patients with PFPS from asymptomatic ones. Thus, the purpose of this study is to find the relationship between the electromyographic (EMG) signals of quadriceps in individuals with and without PFPS during isometric exercises (dissipative and conservative) at different angles of the tibiofemoral joint, to analyze the signal pattern of the control subjects and to compare with the SDFP group. We compared the root means square (RMS) of the EMG signal, median frequency (Fmed) and frequency in 95% of the power spectrum (F95) on the vastus medialis oblique (VMO), vastus lateralis (VL) and rectus femoris (RF) for dissipative and conservative contractions. 24 female volunteers participated in this study (17 asymptomatic patients and 7 with PFPS), who performed 18 contractions at angles of 90º, 60º and 20º of knee extension, using 30% of the maximal voluntary contraction. EMG electrodes were placed on those muscles for data collection, which were further processed using AqDAnalysis software for selection of traits, and MatLab® for processing and analysis of the signal in time and frequency domain. The results indicate that the RMS of EMG signal presents differences among the isometric contractions for the symptomatic group and show that there is a relationship of contraction of a muscle compared to the other when it changes the motor gesture. The Fmed of VMO and VL can be used as a tool in the detection of PFPS, because it acts differently in each group. The F95 of the VMO muscle behaves differently between groups, and during the dissipative isometric contraction at 20º and 60º, their values were higher in PFPS than in the control group. Thus, it was possible to report that it is possible to differentiate the PFPS, or even detect it using EMG parameters of different patterns of isometric contractions

A Síndrome Dolorosa Femoropatelar (SDFP), possui etiologia multifatorial e acomete cerca de 7 a 15% da população, em sua maioria mulheres, jovens, adultas e ativas. Ela causa dor anterior ou retropatelar e é exacerbada durante gestos motores funcionais, tais como subir e descer escadas ou permanecer longos períodos de tempo sentado, agachado ou ajoelhado. Como a avaliação diagnóstica desta síndrome ainda é indireta, diversos mecanismos e metodologias buscam realizar uma classificação que diferencie os portadores de SDFP com relação aos assintomáticos. Deste modo, o objetivo desse trabalho é estudar as relações entre os sinais eletromiográficos (EMG) do músculo quadríceps em indivíduos com SDFP durante exercícios isométricos distintos (dissipativo e conservativo) em diferentes ângulos da articulação tíbiofemoral, e compará-los com os indivíduos sem SDFP. Foram analisadas a intensidade do sinal EMG (RMS), a frequência mediana (Fmed) e a frequência em 95% (F95) do espectro de potência dos músculos vasto medial oblíquo (VMO), vasto lateral (VL) e reto femoral (RF) para as contrações isométricas dissipativa e conservativa. Participaram deste estudo 24 voluntários do sexo feminino (17 assintomáticos e 7 com SDFP) que realizaram 18 contrações nos ângulos de 90º, 60º e 20º de extensão de joelho, utilizando 30% da carga voluntária máxima. Eletrodos EMG foram posicionados nos referidos músculos para a coleta dos dados, os quais foram posteriormente tratados e processados, possibilitando a análises do sinal EMG nos domínio do tempo e da frequência. Os resultados indicam que o RMS do sinal EMG apresenta-se distinto entre as contrações isométricas para o grupo sintomático e evidenciam que não existe uma relação de intensidade de contração EMG de um músculo comparativamente ao outro quando se altera o gesto motor. A Fmed de VMO e VL podem ser utilizadas como ferramenta na detecção de SDFP, visto que atuam de maneira distinta entre os grupos. A F95 do músculo VMO comporta-se de um modo diferente entre os grupos, sendo que durante a contração isométrica dissipativa em 20º e 60º, seus valores em SDFP foram maiores do que no grupo Controle. Desta maneira, foi possível relatar que é possível diferenciar a SDFP, ou até mesmo detectá-la, utilizando parâmetros EMG de padrões diferentes de contrações isométricas. / The Patellofemoral Pain Syndrome (PFPS) has a multifactorial etiology and affects approximately 7-15% of the population, mostly active young women. PFPS causes retropatellar pain, that is exacerbated during motor functional gestures, such as climbing stairs, sitting, squatting or kneeling for long periods of time. Since the diagnostic evaluation of this syndrome is still indirect, different mechanisms and methodologies seek to achieve a classification able to distinguish patients with PFPS from asymptomatic ones. Thus, the purpose of this study is to find the relationship between the electromyographic (EMG) signals of quadriceps in individuals with and without PFPS during isometric exercises (dissipative and conservative) at different angles of the tibiofemoral joint, to analyze the signal pattern of the control subjects and to compare with the SDFP group. We compared the root means square (RMS) of the EMG signal, median frequency (Fmed) and frequency in 95% of the power spectrum (F95) on the vastus medialis oblique (VMO), vastus lateralis (VL) and rectus femoris (RF) for dissipative and conservative contractions. 24 female volunteers participated in this study (17 asymptomatic patients and 7 with PFPS), who performed 18 contractions at angles of 90º, 60º and 20º of knee extension, using 30% of the maximal voluntary contraction. EMG electrodes were placed on those muscles for data collection, which were further processed using AqDAnalysis software for selection of traits, and MatLab® for processing and analysis of the signal in time and frequency domain. The results indicate that the RMS of EMG signal presents differences among the isometric contractions for the symptomatic group and show that there is a relationship of contraction of a muscle compared to the other when it changes the motor gesture. The Fmed of VMO and VL can be used as a tool in the detection of PFPS, because it acts differently in each group. The F95 of the VMO muscle behaves differently between groups, and during the dissipative isometric contraction at 20º and 60º, their values were higher in PFPS than in the control group. Thus, it was possible to report that it is possible to differentiate the PFPS, or even detect it using EMG parameters of different patterns of isometric contractions

Darbo tikslas - nustatyti nenutrūkstamų izometrinių susitraukimų tikslumo ir stabilumo valdymo ypatumus. Tyrimo metu kelti šie uždaviniai: 1) nustatyti ir palyginti vaizdinės grįžtamosios informacijos (VGI) ir skirtingos procentinės jėgos (SPJ) įtaką nenutrūkstamo izometrinio susitraukimo tikslumui, stabilumui ir raumenų (agonisto ir antagonisto) elektriniam aktyvumui; 2) nustatyti ir palyginti mokymosi ir potencijuojamojo krūvio įtaką nenutrūkstamo izometrinio susitraukimo tikslumui ir stabilumui. Buvo tiriami jauni, fiziškai aktyvūs vyrai (n = 8; amžius 20,0 ± 1,5 m, ūgis 182,4 ± 6,5 cm; kūno masė 73,0 ± 5,7 kg, KMI kūno masės indeksas 22,0 ± 1,7 kg/m2 (vid. ± S). Pirmojo tyrimo metu tiriamieji atliko 20%, 50% ir 70% nuo MVJ nenutrūkstamus izometrinius susitraukimus (NIS). Tiriamieji atliko 2 NIS su ir be VGI. NIS truko 13 s, tačiau buvo analizuojami tik paskutinių 10 s duomenys, nes per pirmas 3 s tiriamiesiems buvo leidžiama pasiekti reikiamą jėgą nuo MVJ ir ją išlaikyti likusias 10 s. Antrojo tyrimo metu tiriamieji atliko tokius pačius NIS kaip ir pirmojo tyrimo metu 20% jėga nuo MVJ. Tiriamieji po su VGI ir be jos atliktų NIS atliko rankos raumenų potenciaciją, kurios metu turėjo padidinti rankos lenkimo jėgą iki maksimumo ir ją išlaikyti 10 s. Pailsėję 10 s vėl atliko NIS be VGI. Visas tyrimas buvo pakartotas po 9 - erių mokymosi pratybų, kai per pratybas tiriamieji buvo mokomi atlikti greitus ir tikslius izometrinius susitraukimus 20% jėga nuo MVJ kas antrą dieną su... [toliau žr. visą tekstą] / Research aim was to establish the peculiarities of control of the accuracy and the stability of continuous isometric muscle contractions. Objectives: 1) to establish and compare effect of visual feedback on the accuracy, stability and muscle activity of continuous isometric contractions performed with different strength; 2) to establish and compare effect of learning on the dependence of accuracy and stability of a continuous isometric contraction on muscle potentiation. The subjects studied were healthy, physically active men (n = 8; age 20.0 ± 1.5 m, height 182.4 ± 6.5 cm; body mass 73.0 ± 5.7 kg, body mass index 22.0 ± 1.7 kg/m2 ( ±SD). In experiment 1, the subjects performed CIC at 20%, 50% and 70% force of MVC. The subjects performed two series of CIC – first with visual feedback information (VFI), second – without VFI. The duration of each series was 13 sec. In experiment 2, the subjects performed the same CIC at 20% force of MVC. The subjects after two series of CIC (first with VFI, second – without VFI), done potentiation load (PL), when they had to achieve maximal force and it maintain 10 sec. After 10 sec rest the subjects performed CIC without VFI. All the experiment was repeated after 9 training series. In training series, they were learning speeds – accuracy isometric contractions at 20% force of MVC. Absence of visual feedback information worsening in the accuracy of performing CIC at all forces of MVC. We have established that there is a significant... [to full text]

Study objective - The aim was to determine whether areas in the periaqueductal grey matter, medial dorsal raphé, or ventrolateral medulla might be involved with the integration of blood pressure and heart rate during isometric exercise.Design - Cats were anaesthetised with α chloralose (75 mg·kg-1) and catheters inserted into the right jugular vein and carotid artery. Isometric contractions were generated using a microprocessor controlled stimulator and sleeve electrode around the tibial nerve. Bilateral lesions were made in the dorsal periaqueductal grey matter (P1.0, LR 2.0, HD + 1.5 mm) or two sites in the ventrolateral medulla (P12.0, RL 2.0, HD -10 mm; or P12.0, RL 2.0, HD -8.5 mm). Lesions were also made in the medial dorsal raphé nuclei (P1.0, RL 0.0, HD +1.5 mm). Clonidine was injected into the cerebral aqueduct to determine whether it would exert an antipressor effect during muscle contraction after the lesions were made. Only one site of lesion was made in a group of animals. Bilateral injections of clonidine (250 ng in 0.5 μl) were made into the intact ventrolateral medulla (P11.5, RL 4.0, HD -8.5 mm) to explore its role further. Fatiguing contractions were performed before and after the lesions were made, or clonidine was injected, and changes in arterial blood pressure and heart rate were measured. Verification of the lesion sites or the microinjection sites, and the extent of the lesion or spread of the clonidine, was made from histological examination of brain tissue after each experiment.Experimental material - Adult cats of either sex, n = 20, weight 2.4 (SD 0.4) kg, were used.Measurements and main results - Fatiguing isometric contractions in control conditions caused mean arterial pressure to increase by 45-50 mm Hg and heart rates by 20-25 beats·min-1. Bilateral lesions in the dorsal periaqueductal grey matter did not alter resting mean arterial pressure but attenuated the pressor response during contractions. Injections of clonidine into the cerebral aqueduct had no further antipressor effects after the lesions. Lesions of the medial dorsal raphé nuclei or injections of clonidine into the intact medial dorsal raphé nuclei did not affect the pressor response to fatiguing isometric contractions. Injections of clonidine into the intact ventrolateral medulla eliminated the pressor response to isometric contractions. Bilateral lesions of the ventrolateral medulla near the rostral lateral border of the inferior olivary tract nuclei (P12.0, LR 2.0, HD -10 mm) also attenuated the muscle pressor response, while subsequent injections of clonidine into the cerebral aqueduct depressed the changes in blood pressure further.Conclusions - Ergoreceptor information may be processed through the periaqueductal grey matter through the ventrolateral medulla to control arterial blood pressure during isometric exercise to fatigue.

The purpose of this thesis was to determine whether practice-induced adjustments and retention of a goal directed isometric motor accuracy task were similar between ipsilateral upper and lower limb and whether there is an ipsilateral transfer between upper and lower limbs. In addition, this thesis project aimed to determine whether motor output variability and the activity of the involved agonist and antagonist muscles could predict any of the above stated changes. Sixteen young adults (8 men, 8 women; 22.1 or - 2.1 years) performed 80 trials of goal directed isometric contractions that involved accurately matching a target force of 25% MVC in 200 ms, either with the upper limb or the lower limb followed by the other limb. After an interval of 48 hours, 10 trials similar to the practice trials were performed to examine retention. Feedback of performance was provided in the form of a force-time trajectory along with numerical error values for force and time on each trial. End-point error was quantified as the absolute deviation from the targeted force and time. Motor output variability was quantified as the SD of force, SD of time to peak force and SD of force trajectory. The practice-induced adjustments for force and time endpoint accuracy were similar for the two limbs, however, two days later, retention of the force accuracy was better with the upper limb compared with the lower limb. Practice-induced reduction and practice-to-retention increase in force and time endpoint error were predicted by respective changes in peak force and time to peak force trial-to-trial variability for both limbs. In addition, the changes in accuracy were predicted by the changes in the activity of the involved agonist and antagonist muscles. Nonetheless, the changes in muscle activity differed between the two limbs. The adjustments in muscle activity were also different during the practice session despite the fact that the rate of improvement was similar for the two limbs. Finally, there was an asymmetric transfer of force accuracy from the lower limb to the ipsilateral upper limb, which was associated with the changes in motor output variability. The upper limb, which is inherently less variable as compared to the lower limb, may have retained the task better due to the formation of a stronger muscle synergy (or stronger internal model) to perform the contractions with accuracy. The lower limb, on the other hand may have formed a weaker internal model due to the greater interference from amplified signal-dependent noise (motor output variability) or an alternative motor plan, which may have been concerned primarily with the minimization of motor output variability instead of formation of a muscle synergy to perform the contractions accurately.

Afin d’être représentatif d’un niveau d’effort musculaire, le signal électromyographique (EMG) est exprimé par rapport à une valeur d’activation maximale. Comme l’épaule est une structure articulaire et musculaire complexe, aucune contraction volontaire isométrique (CVMi) proposée dans la littérature ne permet d’activer maximalement un même muscle de l’épaule pour un groupe d’individus. L’objectif de ce mémoire est de développer une approche statistique permettant de déterminer les CVMi optimales afin de maximiser les niveaux d’activation d’un ensemble de muscles de l’épaule. L’amplitude du signal EMG de 12 muscles de l’épaule a été enregistrée chez 16 sujets alors qu’ils effectuaient 15 CVMi. Une première approche systématique a permis de déterminer les 4 CVMi parmi les 15 qui ensemble maximisent les niveaux d’activation pour les 12 muscles simultanément. Ces 4 contractions ont donné des niveaux d’activation supérieurs aux recommandations antérieures pour 4 muscles de l’épaule. Une seconde approche a permis de déterminer le nombre minimal de CVMi qui sont nécessaires afin de produire un niveau d’activation qui n’est pas significativement différent des valeurs d’activation maximales pour les 16 sujets. Pour 12 muscles de l’épaule, un total de 9 CVMi sont requises afin de produire des valeurs d’activation qui sont représentatives de l’effort maximal de tous les sujets. Ce mémoire a proposé deux approches originales, dont la première a maximisé les niveaux d’activation qui peuvent être produits à partir d’un nombre fixe de CVMi tandis que la deuxième a permis d’identifier le nombre minimal de CVMi nécessaire afin de produire des niveaux d’activation qui ne sont pas significativement différentes des valeurs d’activation maximales. Ces deux approches ont permis d’émettre des recommandations concernant les CVMi nécessaires à la normalisation de l’EMG afin de réduire les risques de sous-estimer l’effort maximal d’un ensemble d’individus. / As a means to provide information on the intensity of the muscle activity, the electromyographic (EMG) signal must be expressed as a percentage of a maximum activation value. Because of the anatomical complexity of the shoulder, no single maximum voluntary isometric contraction (MVIC) has been found to maximally activate the same muscle for a group of subjects. The objective of this study is to develop a statistical approach to determine the optimal MVICs that can maximise the activation levels for several shoulder muscles. The amplitude of the EMG signal of 12 shoulder muscles was recorded as 16 subjects performed 15 MVICs in standardised positions. A first systematic approach was used to determine the optimal 4 MVICs amongst 15 that could maximise the activation levels of 12 shoulder muscles simultaneously. The activation levels produced by these 4 MVICs were significantly higher than those produced by previous recommendations. A second approach was adopted to identify the minimum number of MVICs that were required to produce muscle activation levels that did not significantly differ from the maximum activation values. For 12 shoulder muscles, 9 MVICs were necessary to produce muscle activation levels that were representative of the maximum effort of all 16 subjects. This study proposed two novel approaches, the first of which maximised the EMG activation levels that can be produced by a fixed number of MVICs while the second approach identified the minimum number of MVICs required to produce EMG activation levels that were not significantly different from the maximum values of activation. These two approaches provided guidelines with regards to the MVICs that are necessary to normalize the EMG signals of 12 shoulder muscles while reducing the risks of underestimating the maximum capacity of each individual.