Thesis--Princeton University. / Without thesis statement. "Appendix: Epistola cuiusdam Angli, qua asseritur consensus verae religionis doctrinae & caeremoniarum in Anglia, contra vanissimos quorundam cavillos, quibus eandem suis ad plebeculam contionibus impugnare conantur. With an English translation": p. [209]-225. Bibliography: p. [226]-237.

Thesis--Princeton University. / Without thesis statement. "Appendix: Epistola cuiusdam Angli, qua asseritur consensus verae religionis doctrinae & caeremoniarum in Anglia, contra vanissimos quorundam cavillos, quibus eandem suis ad plebeculam contionibus impugnare conantur. With an English translation": p. [209]-225. Bibliography: p. [226]-237.

The genus Evides Dejean, 1833 (Coleoptera: Buprestidae) belongs to the family of woodboring beetles commonly known as jewel beetles. This comparative study was undertaken on the morphological characteristics of three species from field specimens, private collections and collections at the Transvaal Museum of Natural History. The genus Evides consists of eleven species and one subspecies in total worldwide. Nine species and one subspecies are endemic to Sub-Saharan Africa. One species is recorded from India and further species from Laos and Vietnam: Bellamy (World Catalogue, in prep.) The objective of the study was to quantify morphological differences between species representing the genus in South Africa. Specimens of E. gambiensis (Laporte & Gory, 1835), E. interstitialis (Obenberger, 1924) and E. pubiventris (Laporte & Gory, 1835) were collected in the Waterberg (Limpopo Province) and additional specimens of E. pubiventris were collected at Ezemvelo Nature Reserve (Gauteng Province). All three species are related, difficult to differentiate in the field and occur on the same host plants, marula (Sclerocarya birrea (A. Rich.) Hochst.) and false marula (Lannea discolor (Sonder) Engl.). Results indicate that there are morphological differences and species can be differentiated according to elytra length, total elytron width, eye size, frons length, frons width and abdomen length.

A brief overview of the importance of wood boring insects is provided. Background on the two wood boring insect families Buprestidae (Coleoptera) and Sesiidae (Lepidoptera) is given. Keys and checklists to Tennessee’s buprestid fauna as presently known are furnished. Photomicrographs depicting characteristics separating Tennessee buprestid taxa to the level of species are provided for select couplets to aid those unfamiliar with buprestid morphology and terminology. Distribution and flight data of many species within the state are also featured. Results of a phylogenetic analysis of the Chrysobothris femorata (Olivier) species complex is presented based on the nuclear gene arginine kinase and the mitochondrial gene cox I. Implications of the resultant phylogenies are discussed. Phylogenetic relationships with the economically important sesiid tribe Synanthedonini are explored using cox I gene sequences. The cox I tree inferred provides interesting new insight into some ambiguous evolutionary relationships. Morphological characters that are used to distinguish genera within Synanthedonini are discussed and compared with the molecular data.

Thesis is divided into two parts, theoretical and practical which correspond together thematically. The common denominator is communication through art. Theoretical part is dedicated to current communication methods and historical influences on current relationship between society and art and represents artists, who use this dialog in their work. Practical part is dedicated to the communication itself through selected kind of artistic expression, the jewel. The practical part is being processed in a ceramic clay along with various auxiliary material.

This thesis is focused on jewel and his placement in the art education at primary school. The theoretical part focuses on classification and function of jewel in general. It describes his development and monitors the changes of jewellery production from the beginning of the 20th century until now in the Czech Republic and foreign countries. In the theoretical part non-traditional materials used for jewel are described. There is also introduced the textile jewel as a part of jewellery made from non-traditional materials. The practical part includes two methodological art series and the concept of jewel is introduced to children through them. The thesis includes supplementary materials and photo documentation.

Hymenopteran insects, which include all ants, bees and wasps, reproduce through a poorly understood form of reproduction known as haplodiploidy. A promising experimental system for understanding this developmental process is the jewel wasp, Nasonia vitripennis. A critical aspect of using Nasonia as a model is establishing an effective means for editing specific genes of interest so that their functions can be studied through genetic means. For my thesis research, I performed a pilot study of the gene editing method known as CRISPR in Nasonia. I targeted the single heterochromatin protein 1 (HP1) gene present in the Nasonia genome in order to assess the feasibility of this gene editing approach. Targeting HP1 would provide a clear phenotype when this gene is mutated due to its essential functions in early development known from studies in other eukaryotes. Additionally, creating a mutant of this gene will provide a means for studying the role of HP1 in wasp spermatogenesis, an aim that interlinks with the broader chromatin-based goals of our laboratory. Through this study I worked out a streamlined procedure for injecting CRISPR molecules into young wasp embryos, conducting genetic crosses with injected wasps, and screening through their progeny for potential mutants. I observed no mutant phenotypes in injected wasps, but instead, I isolated four potential mutants in F1 progeny. My work has helped to create a solid framework for improving this procedure in Nasonia, and they allow for a better overall understanding of the limitations of producing mutants through CRISPR gene editing in non-model organisms such as Nasonia.

No description available.

A grande procura por mirtilos, principalmente por países do Hemisfério Norte, está associada aos benefícios nutracêuticos do seu consumo em relação a prevenção de doenças. Em resposta à esta alta demanda, produtores de regiões e países que antes não cultivavam a fruta, iniciaram seus plantios nos últimos anos, sendo o clima, fator limitante à expansão de novas áreas uma vez que o mirtileiro, originário de regiões temperadas, necessita acumular alto número de horas de frio hibernal para completar seu desenvolvimento. No Brasil, predomina-se o plantio de mirtileiros nos estados da região sul, além de pequenas regiões de altitude no estado de São Paulo e Minas Gerais, utilizando cultivares tradicionais. A introdução de novos materiais genéticos, de baixa exigência em frio, desenvolvidos pela Universidade da Flórida possibilitou avaliar o desempenho das plantas e a qualidade dos frutos das cultivares Emerald e Jewel plantadas em Piracicaba - SP, sem ocorrência de frio hibernal. No período de maio de 2014 a maio de 2015 foi realizada a caracterização das fases de crescimento vegetativo, brotação, florescimento, frutificação e produção de frutos dessas cultivares. O crescimento dos ramos ocorre até o início da produção, interrompendo-se em seguida. A cultivar Emerald apresenta dois picos de florescimento e frutificação, com a colheita principal ocorrendo de agosto a outubro, enquanto a cultivar Jewel apresenta dois picos de emissão de botão floral, mas apenas um pico de florescimento e uma colheita que ocorre entre os meses de outubro e janeiro. A produção ocorre durante o período de entressafra do mercado interno e externo, que possibilita melhores preços da fruta. É possível obter-se produção das cultivares de mirtileiros Jewel e Emerald em região subtropical, mas a exigência de um número de horas de frio para estimular a emissão de botão floral e o florescimento parece ser mais baixa que a recomendada na literatura. Em agosto e outubro de 2015 também foram determinados para essas cultivares, parâmetros da qualidade físico-química dos frutos como massa fresca de frutos, diâmetro, comprimento, formato, volume, firmeza, coloração de casca, teor de sólidos solúveis totais, pH, acidez titulável, ratio, compostos fenólicos totais, teor de antocianinas, quercetina e atividade antioxidante pelo sequestro dos radicais DPPH e ABTS, que foram armazenados à temperatura ambiente (22 °C) avaliados no dia de colheita, e a cada três dias, até o décimo segundo dia. Frutos de mirtilos colhidos em agosto são maiores e mais arredondados, porém apresentam menor teor de sólidos solúveis totais e maior acidez. Por outro lado, os frutos colhidos em outubro apresentam maior ratio, e coloração mais intensa. \'Emerald\' possui frutos com maior atividade antioxidante que \'Jewel\'. Os teores de antocianinas, quercetinas, compostos fenólicos totais e atividade antioxidante diferem entre as cultivares de acordo com as épocas e aumentam ao longo dos dias após a colheita, enquanto pH, acidez e firmeza decrescem. / The high demand for blueberries, mainly by the Northern Hemisphere markets, is associated with their nutraceutical attributes and the health benefits of its consumption. In response to this high demand, countries that had never cultivated the fruit, started growing blueberries in recent years. The climate is a limiting factor to the expansion of new areas as these species are originated from temperate regions, an so, has a high chill requirement for a full development. In Brazil, blueberry cultivation of traditional areas are restricted to the southern states, and to small areas of altitude in São Paulo and Minas Gerais state, using traditional cultivars. The introduction of new genetic material, with low chill requirement, released by the University of Florida made possible to evaluate the performance of plants and fruit quality of Emerald and Jewel cultivars planted in Piracicaba - SP, without the occurrence of temperatures below 7.2 °C. From May 2014 to May 2015 vegetative growth, bud break, flowering, fruiting and fruit production of these cultivars were assessed. Branches grew until flowering period. Emerald cultivar has two peaks of flowering and fruiting, with the main harvest occurring from August to October, while \'Jewel\' has two bud break peaks, but only a peak of flowering and the harvest takes place between October and January. The production occurs during the off-season of the domestic and northern hemisphere markets, resulting in higher fruit prices. The results indicate that it is possible to cultivate blueberry cultivars Jewel and Emerald in subtropical regions because the chill requirement to induce bud and flowering break is much lower than it is cited in the literature. In August and October 2015 \'Jewel\' and \'Emerald\' were assessed for parameters of physical and chemical quality of the fruit. It were evaluated fresh fruit mass, diameter, length, shape, volume, firmness, peel color, soluble solids content (SST), pH, titratable acidity (AT), SST/AT ratio, total phenolic compounds, total anthocyanins content, total quercetins content and antioxidant activity using DPPH and ABTS methods on the harvest day, and every three days, until the twelfth day of the fruits harvested and stored at room temperature (22 °C). Fruits from August harvest have higher diameter and are more roundish, but have lower soluble solids content and higher titratable acidity. On the other hand, the fruits harvested in October have higher SST/TA ratio and higher chroma value. \'Emerald\' has higher antioxidant activity than \'Jewel\'. The content of anthocyanins, quercetins, total phenolic compounds and antioxidant activity differ with cultivars and harvest periods and increases during storage, while pH, acidity and firmness decrease.

A grande procura por mirtilos, principalmente por países do Hemisfério Norte, está associada aos benefícios nutracêuticos do seu consumo em relação a prevenção de doenças. Em resposta à esta alta demanda, produtores de regiões e países que antes não cultivavam a fruta, iniciaram seus plantios nos últimos anos, sendo o clima, fator limitante à expansão de novas áreas uma vez que o mirtileiro, originário de regiões temperadas, necessita acumular alto número de horas de frio hibernal para completar seu desenvolvimento. No Brasil, predomina-se o plantio de mirtileiros nos estados da região sul, além de pequenas regiões de altitude no estado de São Paulo e Minas Gerais, utilizando cultivares tradicionais. A introdução de novos materiais genéticos, de baixa exigência em frio, desenvolvidos pela Universidade da Flórida possibilitou avaliar o desempenho das plantas e a qualidade dos frutos das cultivares Emerald e Jewel plantadas em Piracicaba - SP, sem ocorrência de frio hibernal. No período de maio de 2014 a maio de 2015 foi realizada a caracterização das fases de crescimento vegetativo, brotação, florescimento, frutificação e produção de frutos dessas cultivares. O crescimento dos ramos ocorre até o início da produção, interrompendo-se em seguida. A cultivar Emerald apresenta dois picos de florescimento e frutificação, com a colheita principal ocorrendo de agosto a outubro, enquanto a cultivar Jewel apresenta dois picos de emissão de botão floral, mas apenas um pico de florescimento e uma colheita que ocorre entre os meses de outubro e janeiro. A produção ocorre durante o período de entressafra do mercado interno e externo, que possibilita melhores preços da fruta. É possível obter-se produção das cultivares de mirtileiros Jewel e Emerald em região subtropical, mas a exigência de um número de horas de frio para estimular a emissão de botão floral e o florescimento parece ser mais baixa que a recomendada na literatura. Em agosto e outubro de 2015 também foram determinados para essas cultivares, parâmetros da qualidade físico-química dos frutos como massa fresca de frutos, diâmetro, comprimento, formato, volume, firmeza, coloração de casca, teor de sólidos solúveis totais, pH, acidez titulável, ratio, compostos fenólicos totais, teor de antocianinas, quercetina e atividade antioxidante pelo sequestro dos radicais DPPH e ABTS, que foram armazenados à temperatura ambiente (22 °C) avaliados no dia de colheita, e a cada três dias, até o décimo segundo dia. Frutos de mirtilos colhidos em agosto são maiores e mais arredondados, porém apresentam menor teor de sólidos solúveis totais e maior acidez. Por outro lado, os frutos colhidos em outubro apresentam maior ratio, e coloração mais intensa. \'Emerald\' possui frutos com maior atividade antioxidante que \'Jewel\'. Os teores de antocianinas, quercetinas, compostos fenólicos totais e atividade antioxidante diferem entre as cultivares de acordo com as épocas e aumentam ao longo dos dias após a colheita, enquanto pH, acidez e firmeza decrescem. / The high demand for blueberries, mainly by the Northern Hemisphere markets, is associated with their nutraceutical attributes and the health benefits of its consumption. In response to this high demand, countries that had never cultivated the fruit, started growing blueberries in recent years. The climate is a limiting factor to the expansion of new areas as these species are originated from temperate regions, an so, has a high chill requirement for a full development. In Brazil, blueberry cultivation of traditional areas are restricted to the southern states, and to small areas of altitude in São Paulo and Minas Gerais state, using traditional cultivars. The introduction of new genetic material, with low chill requirement, released by the University of Florida made possible to evaluate the performance of plants and fruit quality of Emerald and Jewel cultivars planted in Piracicaba - SP, without the occurrence of temperatures below 7.2 °C. From May 2014 to May 2015 vegetative growth, bud break, flowering, fruiting and fruit production of these cultivars were assessed. Branches grew until flowering period. Emerald cultivar has two peaks of flowering and fruiting, with the main harvest occurring from August to October, while \'Jewel\' has two bud break peaks, but only a peak of flowering and the harvest takes place between October and January. The production occurs during the off-season of the domestic and northern hemisphere markets, resulting in higher fruit prices. The results indicate that it is possible to cultivate blueberry cultivars Jewel and Emerald in subtropical regions because the chill requirement to induce bud and flowering break is much lower than it is cited in the literature. In August and October 2015 \'Jewel\' and \'Emerald\' were assessed for parameters of physical and chemical quality of the fruit. It were evaluated fresh fruit mass, diameter, length, shape, volume, firmness, peel color, soluble solids content (SST), pH, titratable acidity (AT), SST/AT ratio, total phenolic compounds, total anthocyanins content, total quercetins content and antioxidant activity using DPPH and ABTS methods on the harvest day, and every three days, until the twelfth day of the fruits harvested and stored at room temperature (22 °C). Fruits from August harvest have higher diameter and are more roundish, but have lower soluble solids content and higher titratable acidity. On the other hand, the fruits harvested in October have higher SST/TA ratio and higher chroma value. \'Emerald\' has higher antioxidant activity than \'Jewel\'. The content of anthocyanins, quercetins, total phenolic compounds and antioxidant activity differ with cultivars and harvest periods and increases during storage, while pH, acidity and firmness decrease.