Depuis le printemps 2019, une campagne de sensibilisation aux enjeux de santé psychologique est déployée sur le campus de l’Université de Montréal. À l’instar de cette campagne, de nombreuses initiatives sont lancées au travers de la province pour enrayer la stigmatisation de la maladie mentale. Ainsi, un nouveau Discours, positif et anti-stigmatisant tente de s’imposer face au Discours stigmatisant ancré dans la société. Par Discours, il faut comprendre un ensemble discursif qui reprend des idéologies et les significations de phénomènes ; celui-ci se matérialise dans les mots et les interactions sociales (discours). On assiste à une mise en tension du sens que l’on donne de la maladie mentale. En s’appuyant sur la théorie de l’étiquetage, ce mémoire a pour objectif de comprendre la tension entre ces deux Discours dans les paroles (le discours) et attitudes des Québécoises et Québécois. La problématique se pose autour des étudiantes et étudiants universitaires du fait de leur tranche d’âge hybride (sortie de l’adolescence et entrée dans l’âge adulte) où la tolérance sociale de la maladie mentale bascule de l’indulgence pour les enfants atteints à une stigmatisation pour les adultes atteints. À cette fin, cette étude a mené des groupes de discussion avec des étudiantes et étudiants d’universités québécoises qui ont vécu une expérience personnelle de la maladie mentale afin de dépeindre leur perception et leur expérience de cette tension discursive dans leurs relations interpersonnelles. Une analyste thématique des transcriptions de ces discussions a été effectuée. Les principaux résultats montrent que pour les participants, il subsiste des traces du Discours stigmatisant dans les paroles des personnes qui les entourent. À des niveaux plus ou moins élevés suivant les personnes de l’entourage, ces traces s’accompagnent d’un éloignement social. Un éloignement qui s’opère en double dynamique et souligne la capacité des personnes souffrant de maladie mentale à interagir dans un nouveau groupe social. / Beginning in the spring of 2019, an awareness campaign on mental health issues has been deployed on the campus of the Université de Montréal. Many similar initiatives have been launched across the province of Quebec that aim to eliminate the stigma of mental illness. Thus, a new, positive and anti-stigmatizing Discourse is challenging the deep-rooted Discourse that stigmatizes mental illness in society. By Discourse, it is necessary to understand a discursive whole that takes up ideologies and the meanings of phenomena; this is materialized in words and social interactions (discourse). There is thus a growing tension inherent to the very meaning of mental illness. The purpose of this Master’s thesis is to better understand this tension between these two Discourses in the words and attitudes of Quebecers. The research project focuses on university students because of their hybrid age bracket (leaving adolescence and entering adulthood) where the societal tolerance for mental illness tends to shift from indulgence for children to stigmatization for adults. To this end, focus groups with Quebec university students allowed us to depict their perception of this discursive tension in their interpersonal relationships. The discussion transcripts were thematically analyzed. The main results show the emergence of a dissonant discourse, which mixes stigmatizing and anti-stigmatizing Discourse, and the persistence of stigmatizing Discourse held by people who are socially distant from the sick person. These findings formed the basis of a relational model of how participants experienced the stigma of their mental illness, providing a new perspective on the theory of labelling and stigmatization.

From an overview of most commonly used kinematic conceptions of robotic manipulators, the conception of Cartesian robot was identified as the most suitable for the given task of colony picking. A control system consisting of two modular parts has been designed for the colony picking robot. ColonyCounter module is a set of image processing libraries for identification of microbial colonies in image data and precise localization of individual colonies. This has been achieved by combination of multiple methods, most importantly connected components labelling and Hough circular transform. The second module – ColonyPicker – utilizes output of ColonyCounter module to plan the picking and placing of colonies. Subsequently it controls the transfer process itself using an innovative task planning and executing system.

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NK cells are innate lymphocytes which constitute the first line of organism's defence against infections through their receptor system. These cells represent an important part of antiviral and antitumor immunity, they also play a role in transplant immunity, autoimmunity and reproduction. This diploma thesis inquires into the structure of the transmembrane receptor NKR-P1B of mouse NK cells and the interaction with its ligand Clr-b. The aim was to prepare the expression vector coding the ligand-binding and whole extracellular region of the receptor NKR-P1B and to optimize its production and refolding in vitro. Purified protein samples were analyzed by size-exclusion chromatography, electrophoresis and mass spectrometry. Interaction between NKR-P1B and Clr-b proteins was tested using biophysical (size-exclusion chromatography and surface plasmon resonance) and biological methods (labelling of cellular sample with NKR-P1B proteins marked with fluorescent dye). In vitro binding experiments have not confirmed mutual interaction between NKR-P1B and Clr-b despite the prepared proteins binding to the bone marrow cells.

Cytokinins are a large group of phytohormones. Since their discovery in the 1950s, they have shown to play a pivotal role in plant physiology. Most studies so far focused on cytokinin action mechanisms and their metabolic regulation. Identification of AtABCG14 and AtPUP14 as cytokinin-specific membrane carriers brought researchers' attention to cytokinin membrane transport, too. In this thesis, we performed experiments with radio-labelled cytokinin tracers. We show that trans-zeatin and isopentenyladenine, two major biologically active cytokinins, are readily transported across the plasma membrane in tobacco BY-2 cell suspension. Making use of mathematical modelling, we show that BY-2 cells possess a membrane transport system with an affinity toward cytokinins. Next, we show that atabcg14 and atpup14 mutations affect cytokinin metabolism in Arabidopsis thaliana plants. Keywords: cytokinin, Arabidopsis thaliana, tobacco BY-2 cell lines, membrane transport, purine permease, ATP-binding cassette, radio-labelling

Mitochondrial isocitrate dehydrogenase 2 (IDH2) catalyzes reductive carboxylation (RC, reverse Krebs cycle pathway) and 2HG synthesis (2HG) - metabolite of which many scientists are interested. 2HG may be concurrently synthetized in cytosol by IDH1. RC is involved in anabolic reactions necessary for cell proliferation - produces citrate, fatty acid precursor - especially in hypoxia. IDH2 and IDH1 are not the only enzymes that are involved in 2HG synthesis. Recently, several enzymes, which participate in 2HG production, have been discovered. 2HG is useful in cancer diagnostics due to its overproduction by transformed cells. Moreover, 2HG may cause epigenetic changes via inhibition of 2-oxoglutarate dependent dioxygenase. In this work, the importance of RC and 2HG synthesis in cancer and healthy cells was investigated by gas chromatography with mass spectrometry detection as well as IDH2 influence. We found that IDH2 significantly participates in reverse RC and 2HG synthesis in breast cancer cell lines and uses glutaminolysis as a supplementary anaplerotic pathway. RC is increased by hypoxia, inhibition of respiration, and decreased by activation of respiration or hypocapnia. We confirmed 2HG synthesis and RC in healthy cells (fibroblasts, breast epithelial cells etc.) as well as in cancer cells....

Die in der vorliegenden Arbeit untersuchten Metall-Keramik-Verbunde wurden mittels Pulverspritzgießen hergestellt. Unter Anwendung der teilautomatisierten Verfahrensoptionen Mehrkomponentenspritzgießen und Inmould-Labelling, welches u. a. die Verwendung tiefgezogener Grünfolien beinhaltete, wurden hierzu 2K-Prüfkörpergeometrien (Zugstab, Biegebruchstab, Ringverbund) und 2K-Demonstratoren (Innenzahnrad, Fadenführer, Greifer) jeweils bestehend aus Stahl 17-4PH und ZrO2 (3%Y2O3), im Co-Sinterverfahren unter H2-Atmosphäre bei 1350°C, entwickelt. Schlüssel zur Darstellung schwindungskonformer ZrO2- und Stahl 17-4PH-Formgebungsmassen war der Angleich der Pulverpackungsdichte. Untersucht wurde neben der Werkstoff- und Gefügeausbildung das sich während dem Formgebungs- und Sinterprozess ausbildende Metall-Keramik-Interface sowie die sich bevorzugt in diesem Bereich manifestierenden Verbundeigenspannungen. Neben der stoffschlüssigen Versinterung beider Partner konnte eine Steigerung der Verbundfestigkeit durch Legierungsmodifikation unter Ausschluss technologischer Fehlerquellen erreicht und spezifiziert werden.:1 Einleitung und Zielstellung .................................................................................................5 2 Stand der Technik ..............................................................................................................6 2.1 Metall-Keramische-Verbundwerkstoffe und Werkstoffverbunde.....................................6 2.2 Werkstoffsystem ............................................................................................................6 2.2.1 Oxidkeramische Metall-Keramik-Verbunde.................................................................9 2.2.2 Nichtoxidkeramische Metall-Keramik-Verbunde........................................................15 2.3 Metall-Keramik-Interface..............................................................................................17 2.3.1 Stahl-Keramik-Komposite.........................................................................................21 2.3.2 Stahl-Keramik-Schichtverbunde................................................................................25 2.4 Konventionelle Verbindungs- und Fügetechnik.............................................................27 2.4.1 Kraft- und Formschluss.............................................................................................28 2.4.2 Lösbare Verbindungen .............................................................................................28 2.4.3 Nicht lösbare Verbindungen .....................................................................................29 2.4.4 Stoffschlüssige Verbindungen ..................................................................................30 2.5 Pulvertechnologische Verbindungs- und Fügetechnik ...................................................32 2.5.1 Co-Shaping..............................................................................................................34 2.5.2 Co-Firing..................................................................................................................38 2.6 Pulverspritzgießen........................................................................................................42 2.6.1 Prozesskette.............................................................................................................43 2.6.2 Werkstoffe...............................................................................................................45 2.6.3 Verfahrenscharakteristik...........................................................................................46 2.6.4 PIM in der industriellen Praxis ...................................................................................48 2.6.5 Mehrkomponentenspritzguss...................................................................................49 2.7 Prüfung und Spezifikation für spritzgegossene Metall-Keramik-Verbunde.....................52 2.7.1 zerstörende Prüfverfahren........................................................................................52 2.7.2 zerstörungsfreie Prüfverfahren .................................................................................55 2.7.3 Prädikative Methoden ..............................................................................................55 3 Experimenteller Teil..........................................................................................................57 3.1 Pulveranmusterung ......................................................................................................57 3.1.1 Feedstockherstellung und Charakterisierung ............................................................58 3.1.2 Grünfolienherstellung und Charakterisierung ...........................................................60 3.1.3 Thermische Analyse..................................................................................................62 3.2 Fertigungstechnologie..................................................................................................62 3.2.1 2-Komponentenpulverspritzgießen...........................................................................64 3.2.2 Folienhinterspritzen..................................................................................................64 3.2.3 Entbinderung und Sinterung ....................................................................................65 3.3 Werkstoff- und Verbundspezifikation...........................................................................66 3.3.1 Bestimmung der Dichte............................................................................................66 3.3.2 Dilatometrie.............................................................................................................66 3.5.2 Optische Interfaceanalyse.........................................................................................67 3.5.3 Mechanische Festigkeit ............................................................................................67 3.5.4 Röntgenographische Eigenspannungsanalyse ...........................................................68 4 Ergebnisdiskussion ...........................................................................................................70 4.1 Werkstoff- und Pulverauswahl .....................................................................................70 4.1.1 Untersuchungen zum Co-Sinterverhalten von Metall- und Keramikpulvern........................................................................................................78 4.1.2 Werkstoff- und Gefügeausbildung während der Co-Sinterung .................................85 4.2 Feedstock- und Bindersystem .......................................................................................92 4.2.1 Rheologische Eigenschaften .....................................................................................95 4.2.2 Thermisches Verhalten und Entbinderung ................................................................99 4.2.3 Verarbeitung von Feedstock und Grünfolie.............................................................101 4.3 Prüfkörperentwicklung...............................................................................................105 4.3.1 Gestaltungsoptionen..............................................................................................105 4.3.2 Verfahrensverifizierung ..........................................................................................106 4.3.3 Qualitative Bewertung der Verfahrensoption Inmould-Labelling..............................109 4.4 Werkstoffverbund.........................................................................................................112 4.4.1 Metall-Keramik-Interface........................................................................................112 4.4.2 Zugfestigkeit..........................................................................................................119 4.4.3 Verbundeigenspannungen .....................................................................................122 5 Zusammenfassung.........................................................................................................126 6 Literaturverzeichnis ........................................................................................................130 7 Abkürzungsverzeichnis...................................................................................................140 Anhang ................................................................................................................................141 A1 Spezifikation ZrO2-Feedstock Z1 .................................................................................142 A2 Spezifikation Stahl-17-4PH-Feedstock M1 ..................................................................143 A3 Rezeptur ZrO2-Folien ..................................................................................................144 A4 Rezeptur Stahl 17-4PH-Folien.....................................................................................145 A5 Prozessparameter – Spritzgießen (Bsp. Biegebruchstab 7x7x70mm)............................146 A6 Folienkonfektionierung – Bsp.- Demonstrator Greifer .................................................147 A7 Prozessautomatisierung – Bsp. Demonstrator Fadenführer..........................................148 A8 Spritzgegossene Demonstratoren – 2K-Spritzgießen...................................................149 A9 Spritzgegossene Demonstratoren – Inmould-Labelling................................................150 A10 Dilatometerschaubilder ..............................................................................................151 A11 Mikrozugproben ........................................................................................................152 A12 studentische Arbeiten ................................................................................................153

Dynamics of carbon and phosphorus flows in arbuscular mycorrhizal symbiosis Mgr. Tereza Konvalinková (doctoral thesis) Abstract Arbuscular mycorrhizal fungi (AMF) are widespread and highly specialized root symbionts, which gain all of their carbon (C) from the hosts, supplying plants with mineral nutrients (particularly with phosphorus, P) in return. This thesis focuses on the size and flexibility of C and P flows in arbuscular mycorrhiza in relation to environmental conditions, in particular to light and P availability. The indications that the symbiotic flows are regulated actively by both partners are discussed. The main findings are presented as a compilation of separate scientific works (two research articles, one review and one book section). A glasshouse experiment has shown that both mycorrhizal benefits and mycorrhizal colonization of medic (Medicago truncatula) by an AMF species (R. irregularis) decline along the gradient of decreasing light intensity. Interestingly, morphological adaptation of medic to the long-term light deprivation was boosted by mycorrhiza, probably because of C demand of AMF and due to the improved nutrition of the mycorrhizal plants. On the other hand, sudden 6-day shading caused rapid decline of shoot P content of mycorrhizal plants, accompanied with the accumulation of P...

In the midst of climate change and growing concern about social aspects, investors want to make informed sustainable choices regarding their consumption and investments. Many companies are trying to stay ahead of the curve by engaging in Corporate Social Responsibility. Mutual funds have noticed this trend and subsequently have started to offer ethical mutual funds as a result. These ethical claims are difficult to scrutinize for investors creating a problem of asymmetric information. This study analyses how ethical claims and how eco-labels, in this case, the Nordic Swan Ecolabel relate to demand for equity mutual funds. In a world where more investors are seeking ethical investments, how is ethical commitment communicated in a trustworthy way? Data on daily Net Asset Value (NAV) and monthly Total Net Assets (TNA) between 2016-01-01 and 2019-12-31, for 217 equity mutual funds sold on the Swedish market were collected from Thomson & Reuters database Eikon. These mutual funds were categorized into three groups, conventional, non-labelled ethical, and eco-labelled mutual funds. The data was structured as panel data and both random effect and fixed effect models were used to estimate the factor loadings. The study shows that Nordic Swan Ecolabelled mutual funds tend to experience higher demand than both non-labelled ethical mutual funds and conventional mutual funds. In other words, the Nordic Swan Ecolabelled group distinguishes itself from the other mutual fund groups. Hence, the results of the study suggest that the Nordic Swan Ecolabel sends a signal that relates positively to the demand for mutual funds. Further, the results implicate that mutual fund companies that aim to introduce mutual funds that are truly ethical or sustainable should consider acquiring the Nordic Swan Ecolabel to signal their sustainability commitment since the results suggest that Nordic Swan Ecolabelled mutual funds have a positive relation to demand, both compared to conventional mutual funds and non-labelled ethical mutual funds. / I en tid präglad av klimatförändring och ökande medvetenhet om sociala aspekter vill investerare ta informerade beslut angående sin konsumtion och angående sina investeringar. Många företag försöker ligga i framkant för en hållbar utveckling genom att ägna sig åt Corporate Social Responsibility. Fondföretag har märkt av denna trend och har därför börjat erbjuda etiska fonder till sina kunder. Huruvida dessa fonder faktiskt är etiska kan vara svårt för investerare att undersöka vilket skapar asymmetrisk information mellan fondföretag och investerare. Denna studie analyserar hur aktiefonders påståenden om att investera etiskt relaterar till efterfrågan på dessa aktiefonder och hur eko-märkningar, i detta fall Svanen-märkningen relaterar till aktiefonders efterfrågan. Hur ska fonders etiska åtaganden kommuniceras på ett trovärdigt sätt i en värld där mer och mer investerare söker sig till etiska investeringar? Dagligt nettoandelsvärde (NAV-kurs) och månatlig fondförmögenhet (TNA) för 217 aktiefonder sålda på den svenska marknaden under tidsperioden 2016-01-01 och 2019-12-31 samlades in från Thomson & Reuters databas Eikon. Dessa fonder delades in i tre grupper, dessa var konventionella, icke-märkta etiska fonder samt eko-märkta fonder. Datan strukturerades som paneldata och både random effects och fixed effects modeller användes för att estimera faktorerna i regressionen. Studien visar att Svanenmärkta fonder tenderar att ha högre efterfrågan än både icke-märkta etiska fonder och konventionella fonder. Med andra ord så utmärker sig de Svanen-märkta fonderna jämfört med de andra grupperna. Resultatet av studien tyder därför på att Svanen-märkningen sänder en signal som relaterar till efterfrågan för en fond. Resultatet av studien implicerar även att fondföretag som ämnar att introducera fonder som faktiskt uppfyller de etiska och hållbara krav de påstår sig göra, bör överväga att förvärva Svanen-märkningen för att signalera sina etiska och hållbara åtaganden, då resultatet föreslår att Svanenmärkta fonder har en positiv relation till efterfrågan, både jämfört med konventionella fonder och icke-märkta etiska fonder.

For 500 years transient light phenomena (TLP) have been observed on the lunar surface by ground-based observers. The actual physical reason for most of these events is today still unknown. Current plans of NASA and SpaceX to send astronauts back to the Moon and already successful deep-space CubeSat mission will allow in the future research nanosatellite missions to the cislunar space. This thesis presents a new hardware and software concept for a future payload on such a nanosatellite. The main task was to develop and implement a high-performance image processing algorithm which task is to detect short brightening flashes on the lunar surface. Based on a review of historic reported phenomena, possible explanation theories for these phenomena and currently active and planed ground- or space-based observatories possible reference scenarios were analyzed. From the presented scenarios one, the detection of brightening events was chosen and requirements for this scenario stated. Afterwards, possible detectors, processing computers and image processing algorithms were researched and compared regarding the specified requirements. This analysis of available algorithm was used to develop a new high-performance detection algorithm to detect transient brightening events on the Moon. The implementation of this algorithm running on the processor and the internal GPU of a MacMini achieved a framerate of 55 FPS by processing images with a resolution of 4.2 megapixel. Its functionality and performance was verified on the remote telescope operated by the Chair of Space Technology of the University of Würzburg. Furthermore, the developed algorithm was also successfully ported on the Nvidia Jetson Nano and its performance compared with a FPGA based image processing algorithm. The results were used to chose a FPGA as the main processing computer of the payload. This concept uses two backside illuminated CMOS image sensor connected to a single FPGA. On the FPGA the developed image processing algorithm should be implemented. Further work is required to realize the proposed concept in building the actual hardware and porting the developed algorithm onto this platform.