Instability waves in a free shear layer formed by two parallei compressibie streams are analyzed using the linear spatial stability theory. Both viscous and inviscid disturbances are considered. The basic state is obtained by solving the compressibie laminar boundary-layer equations or is specified by the hyperbolic tangent velocity profile. The effects of viscosity, Mach number, the velocity and temperature ratios on the growth rate are determined. Unlike the boundary layer flow, viscosity has a stabilizing effect on the mixing layer flow. Increasing the temperature ratio produces a strong stabilizing effect on the growth of the mixing flow; this stabilization does not, however, persist at higher Mach numbers. Whereas the maximum growth rate of the Incompressible mixing layer varies linearly with the velocity ratio, the maximum growth rate of the compressible mixing flow varies nonlinearly with the velocity ratio. The numerical results substantiate the fact that the convective Mach number Is the appropriate parameter for correlating the compressibility effects on the spreading rate of the mixing layer. The ratio of the spreading rate of a compressible layer to that of an incompressible layer at the same velocity and density ratios depends primarily on the convective Mach number. Three-dimensional waves become important when the convective Mach number is greater than 0.6. The influence of nonparallelism on the spatial growth rate of two-dimensional disturbances is evaluated and is found to be negligible. Linear subharmonic Instabilities of a compressible mixing layer, which Is spatially periodic in a translating frame of reference, are analyzed by using Floquet theory. The basic state is obtained by the linear superposition ofa steady mean flow, which is given by a solution to the compressible boundary-layer equations or by a hyperbolic tangent velocity profile approximation, and the neutral primary wave of that mean flow. The results show that the growth rates of two-dimensional subharmonic instabilities (pairing mode) increase with increasing amplitude of the periodicity but decrease with increasing the convective iVIach number. In the incompressible flow case, the most amplified subharmonic wave is a two-dimensional mode, which is in agreement with the published results. For subsonic convective Mach numbers, the presence of the periodicity enhances the growth rates of three-dimensional subharmonic waves over a wide range of spanwise wavenumber which shows a preferred band over which the growth rate is maximum. However, when the convective IVIach number is greater than one, the interaction between the subharmonic wave and the primary wave marginally increases the maximum growth rate of the subharmonic. Nevertheless, that interaction dramatically increases the range of amplified spanwise wave numbers. Fourth-order compact finite-difference codes are developed for solving the compressible boundary-layer equations and investigating their primary and subharmonic instabilities. The codes proved to be very accurate and versatile. / Ph. D.

Static pressure and mean velocity data were obtained in a relaxing shear driven three-dimensional incompressible turbulent boundary layer flow produced by a swept rectangular step. The nominally 10 cm (4 in.) thick boundary layer had a freestream velocity of approximately 25 m/sec (80 ft/sec). The two steps investigated were each 3.8 cm (1.5 in.) high by 18.4 cm (7 .25 irt.) long and at angles of 30° and 45° to the transverse wind tunnel direction. Pressure gradients were determined by taking the derivative of least-squares curve fits to the static pressure data. Close to the trailing edge reattachment region, the maximum·gradient was·0.8 kPa/m (5 psf/f) for the 30° step and 0.4 kPa/m (2.5 psf/f) for the 45°step. As expected, a region of nominal pressure gradient (0.03 kPa/m or 0.2 psf/f compared to 1.6 kPa/m or 10 psf/f for a pressure driven flow) was found at greater than 36 cm (14 in.) down.stream of the trailing edge of each step. The wall crossflow angle decayed from 67° at 15 cm (6 in.) behind the trailing edge to 9° at 66 cm (26 in.) for the 30° step. In the same region, the crossflow angle decayed from 45° to 6° for the 45° step. The decay or relaxation was found to be much faster in the near-wall region and in the region close to the trailing edge. A defect in the streamwise velocity profiles indicated that the flow was dominated by the separation and reattachment over the step. For future shear driven investigations, a lower, more streamlined wing-type body is recommended to produce a moderately skewed boundary layer without dominant separation effects. / Master of Science

Static pressure, mean velocity, indirect wall shear from Preston tubes, direct wall shear using a two-dimensional (single line of action) floating element device, and direct wall shear measurements from an omnidirectional floating element capable of simultaneously determining magnitude and direction of a wall shear vector were completed over a modest range of two-dimensional, (near-zero) pressure gradient flows. Static pressure, mean velocity, and direct wall shear measurements using the omnidirectional meter were completed in a pressure driven, and two different shear driven three-dimensional flows. These data were combined to evaluate ten of eleven three-dimensional similarity models found in the literature. Uncertainty estimates on all the data are presented. Two-dimensional experimental results show that the constants in the two-dimensional law of the wall formula appear to be slightly dependent on Reynolds number, and the Patel calibration formulas for Preston tubes to be better than any other available formulas. Three-dimensional results show (1) the Perry and Joubert and the White, Lessmann, and Christoph three-dimensional similarity models to give limited but overall better agreement with experimental data, (2) none of the proposed models adequately model experimental results for y⁺ < 50, (3) near-wall collateral flow does not exist, and (4) pressure gradient effects on the omnidirectional meter appear to be negligible. / Ph. D.

Corrosion in reinforced concrete bridge decks has always been a concern amongst engineers. However, as structures continue to increase in size and in the amount of reinforcement present, consideration must be given to parameters such as the clear spacing arrangements between bars, the presence and absence of stay-in-place (SIP) forms, and differences in the cathode bar to anode bar ratios. Limited research has been performed to determine the effects of the parameters (Shiessel, P. 1986). Research has been conducted on the effects of macrocell corrosion compared to microcell corrosion. Previous studies have shown that the measured microcell corrosion is not augmented greatly by the macrocell current (Andrade et al. 1991). In this study, twenty-seven specimens were cast with reinforcing steel to represent reinforcing mats at the top and bottom of each specimen. Top and bottom spacing arrangements were approximately 51, 76, 102 mm (2, 3, and 4-inches), cathode-to-anode bar (C/A) ratios were 2 and 1, and the presence and absence of SIP were considered. Macrocell currents, resistivity measurements, half-cell potential measurements, and corrosion current densities were recorded over a 273 day time period to compare the differences that existed amongst the three different parameters. Based upon the data that was collected, no significant differences were recorded when comparisons were made between the spacing arrangements, the absence and presence of SIP, and differences in C/A ratios. The formation of the passive layer was confirmed by the corrosion current densities and half-cell potentials. The rate of the formation of the passive layer occurred in two distinct periods, a rapid rate from casting to about 105 days and a significantly slower rate beyond 105 days after casting. There was no detected influence of the macrocell activity on the formation of the passive layer throughout the 273 day study period. / Master of Science

No / RATIONALE: 5,6-Dimethylxanthenone-4-acetic acid (DMXAA) is a low molecular weight drug of the flavonoid group, which has an anti-vascular effect in tumours causing endothelial cell apoptosis and activation of cytokines. Flavonoid-based compounds have been reported to lead to an upregulation in the expression of lysophosphatidylcholines (LPC)-type lipids in solid tumours. A study employing TLC/MALDI-MS and MALDI-MS imaging to examine LS174T colorectal adenocarcinoma xenografts following administration of DMXAA has been conducted into this effect. METHODS: LS174T colorectal adenocarcinoma xenografts grown in male immune-deficient mice were treated with 27.5 mg/kg DMXAA. The control (before treatment) and 4 h and 24 h post-treatment tumours were excised and divided into two. MALDI-MS imaging experiments were carried out on 12 microm cryosections sections taken from one half of the tumours and from the other half the lipids were extracted and analysed by TLC/MALDI-MS. These experiments were carried out in triplicate. RESULTS: Statistical analysis of the MALDI-MS imaging data set indicated an increased amount of LPC in the 24 h post-treated sample and a decreased amount of PC in the 24 h post-treated sample, compared with the 4 h post-treated sample and the control. These effects were confirmed by the TLC/MALDI-MS data. The lipid extracts were separated into six spots on the TLC plate. These were identified as arising from different lipids classes, i.e. LPC, sphingomyelins (SM), phosphatidylcholines (PC) and phosphatidylethanolamines (PE). The TLC/MALDI-MS data indicated that LPC were highly expressed in the 4 h and 24 h post-treated tumour samples compared with the control. Examination of the mass spectrometric images confirms this increase and demonstrates additionally that the increase in the signals arising from LPC appears to be localised primarily within the central areas of the xenograft. CONCLUSIONS: An increase in expression of LPC lipids in solid tumours treated with DMXAA has been demonstrated and shown to be localised in the central area of the tumour.

Wind speed data of multi-heights have been examined to investigate the spatial and temporal characteristics of coherent structures in the near neutral marine atmospheric surface layer. With Taylor's hypothesis, the temporal velocity signals have been transformed to spatial fluctuations and then visualize these spatial velocity fluctuations to identify the coherent structures. It has been confirmed that there exist similar coherent structures in the marine atmospheric surface layer to those in laboratory turbulent boundary layer. These similar coherent structures include ejections, sweeps, shear layers, transverse vortices, and combined events of the shear layers and transverse vortices. Besides these similar coherent structures, there exist the plume and downdraft motions in the unstable marine atmospheric surface layer. It has been observed that the streamwise spatial length of the ejections and sweeps is 20-250 m and their mean frequency is of order of 0.01-0.001 /s at mean wind speed of 5-12.6 m/s. Between the region of the upstream ejection and downstream sweep motions an inclined shear layer is often seen. The inclined angle of the shear layer has been observed to vary from 30 to 70 degree with the height and length of the the shear layer. The transverse vortices are seen to exist in every region from the wall up to a height of 45 m and their diameter is up to 40 m. The mean frequency of the shear layers and the transverse vortices is of order of 0.001 /s. In the fully developed stage of the combined event of the shear layer and transverse vortex, the shear layer is generally longer and the diameter of the transverse vortex is larger. The mean frequency of the combined event of the shear layers and the transverse vortices is of order of 0.001 /s. The streamwise spatial length of the plume and downdraft motions is generally from 20 m to 50 m. Analysis indicates that the mean wind speed is a dominant factor in affecting the spatial and temporal characteristics of the coherent structures in the near neutral marine atmospheric surface layer. As the mean wind speed increases, the frequency of the shear related coherent events will increase, while the frequency of the buoyancy related coherent events (plumes and downdrafts) will decrease. The temperature difference between higher level of the surface layer and sea surface is the second main factor in affecting the spatial and temporal characteristics of the coherent structures. As the marine atmospheric surface layer becomes more stable the coherent motions will be suppressed. The effect of the temperature difference on the buoyancy related plume and downdraft motions is more evident than on the other shear related coherent motions. / Master of Science

The effects of boundary-layer suction in stabilizing the laminar boundary layer were considered in this experimental work. The experiments were conducted on a flat-plate wind-tunnel model in the V.P.I. & S.U. Stability Tunnel. The capabilities of the wind tunnel in terms of mean flow uniformity and low turbulence level were established and the basic flat-plate experiment was verified by comparison with the well known stability results for the Blasius boundary-layer. The two-dimensional Tollmein-Schlichting waves were introduced into the boundary layer using a vibrating ribbon. Suction was applied through the model surface vra two porous panels which could be located at various streamwise positions on the model. The porous Dynapore surface material of these panels was adapted by Douglas Aircraft Co. for possible use as a wing surface material on aircraft equipped with Laminar Flow Control. Detailed mean-flow measurements without suction showed that the Dynapore had no destabilizing effects in the laminar boundary layer. Using the porous panels, suction could be applied either continuously or in a discrete fashion through spanwise suction strips. With suction applied through a single spanwise strip, the mean-flow effects were determined in terms of the boundary-layer shape factor. Measurements of the disturbance behavior with suction were conducted to determine the relative merits of spatially continuous suction versus suction applied through various discrete suction strip configurations. These measurements were conducted in terms of the integral across the boundary layer of the streamwise disturbance amplitude, that is A = ∫ u' I dy. These results were compared with the theory of Reed & Nayfeh (1981). A method was also proposed by this theory for optimization of suction strip placement, and measurements were made which provided partial confirmation as to the validity of this optimization scheme. Experiments were also conducted without suction to investigate the weak nonlinear two- and three-wave interactions which occur at higher wave amplitudes. In these experiments, two-frequency Tollmein Schlichting waves were introduced simultaneously using a single vibrating ribbon, and initial disturbance amplitudes were controlled. In particular, these experiments established appropriate initial conditions for comparison with analytical models and considered the role of the difference frequencies which were generated through the nonlinear interaction of the two fundamental waves. This work also considered the behavior of the harmonic waves in the nonlinear regime. / Ph. D.

The bio-enabled syntheses of functional nano-structured metal oxide thin films is of importance for a range of applications, in photonics, electronics, sensing, cell engineering, and biochemical devices. This type of novel syntheses method can overcome problems common in conventional oxide processing. In general, conventional oxide processes often require thermal treatment, caustic chemicals, and mechanical processing when producing shape-controlled inorganic materials. In contrast, biological processes are usually carried out under mild conditions (low temperature, neutral pH, and atmospheric pressure) and are therefore promising for the development of benign processes. Functional materials synthesized at room temperature using biomolecules are promising due to their expediency. During recent years, significant discoveries and progress have been made in discovering, and finding new applications for such biomimetic oxide-based minerals. However, much of the research has focused on SiO- and TiO-bearing organic-inorganic hybrid materials, of which a significant limitation is that, there are relatively few water-soluble inorganic oxide precursors commercially available for such biological syntheses. Two common compounds that are used in the biomimetic syntheses of SiO₂ and TiO₂ are tetramethoxisilane (TMOS) and Ti(IV) -bis(ammonium lactato) dihydroxide ( TiBALDH ). As a result, approaches to synthesize new water-soluble transitional metal complexes for use as precursors in the biomineralization of the corresponding functional metal oxide thin films were explored in this work, in order to expand the range of functional oxide chemistries formed via bio-enabled methods. A Ti-containing compound was synthesized to compare the behavior of commercially-available and as-synthesized TiBALDH. Another titanium-containing complex with citrate ligands, instead of lactate, was also synthesized to investigate the influence of the ligand type on the deposition behavior of the precursors. Zirconium- and hafnium-containing complexes were also synthesized to demonstrate the feasibility and versatility of the idea of applying bio-enabled syntheses to the fabrication of functional mineral oxides other than the reported SiO₂ and TiO₂. The second part of this thesis focuses on developing a novel way to fabricate porous functional mineral oxide thin films with controlled pore size, which can be used in a variety of applications, such as dye loading for optical, photochemical, or electrochemical purposes. Commercially-available, carboxyl-group-terminated polystyrene spheres of different sizes were utilized as pore-size controllers in the bio-enabled syntheses of TiO₂ by protamine. This approach has been found to be an effective means of creating uniform pores in inorganic mineral oxide coatings. The accomplishments of this work have the potential to be integrated so as to expand the boundaries of biomineralization in materials science and engineering fields.

<p>In this thesis various descriptions of the near surface atmospheric flow over a high latitude glacier is used in an effort to increase our understanding of the basic flow dynamics there.</p><p>Through their contribution to sea-level change, mountain glaciers play a significant role in Earth’s climate system. Properties of the near surface atmospheric flow are important for understanding glacier response to climate change.</p><p>Here, the near surface atmospheric flow is studied from several perspectives including the effects of both rotation and slope. Rotation is an important aspect of most atmospheric flows and its significance for mesoscale flows have gained recognition over the last years. Similarly, the very stable boundary layer (VSBL) has lately gained interest. Within a VSBL over sloping terrain katabatic flow is known to be usual and persistent. For the present thesis a combination of numerical and simple analytical models as well as observations from the Vatnajökull glacier on Iceland have been used. The models have continuously been compared to available observations. Three different approaches have been used: linear wave modeling, analytic modeling of katabatic flow and of the Ekman layer, and numerical simulations of the katabatic flow using a state of the art mesoscale model. The analytic models for the katabatic flow and the Ekman layer used in this thesis both utilizes the WKB method to allow the eddy diffusivity to vary with height. This considerably improves the results of the models. Among other findings it is concluded that: a large part of the flow can be explained by linear theory, that good results can be obtained for surface energy flux using simple models, and that the very simple analytic models for the katabatic flow and the Ekman layer can perform adequately if the restraint of constant eddy diffusivity is relieved.</p>

The processing and properties of novel polymer-based nano-composite (PNC) dielectrics for capacitor applications has been studied. PNCs were fabricated via a vacuum based deposition technique and their micro/nano-structure, chemical and dielectric properties investigated. After process development and optimisation, co-deposited Al and nylon-6 PNCs had a dielectric constant k&sim;7 at an approximate Al volume fraction of 0.3 that agreed with analytical predictions if it was assumed that the Al transformed to an oxide in-situ and/or after deposition. The significant effect of absorbed water vapour and temperature on PNC dielectric properties was revealed using different types of post-deposition heat treatment. Alternately-deposited PNCs consisting of Al or Ag 2-20 nm layers sandwiched between nylon-6 layers were fabricated in which the overall PNC Al or Ag volume fraction was controlled by varying the nominal Al or Ag layer thickness. Ag layers comprised of discrete nano-islands that produced a nano-capacitor network effect that increased k to &sim;11. In the case of Al layers, when the layer thickness was &ge; 5 nm, corresponding to a nominal volume fraction of 0.1, Al (core)-oxide (shell) nanoparticles were formed and the PNC dielectric constant increased to &sim;19. The detailed nano-structure of the core-shell particles was studied using various types of transmission electron microscopy (TEM), and the elevations in dielectric constant ascribed to multiple-interface polarisation effects dependent on the formation of the core-shell structure. PNCs based on alternate deposition of Ti sandwiched in nylon-6, and then both Ti and Ag in nylon-6 were also fabricated, with k reaching &sim;73 for Ag+Ti/nylon-6 PNCs. As well as Ti-based core (metal)-shell (oxide) particles, the Ag volume fraction was sufficiently high in the 10 nm nylon-6 layers to again form a nano-capacitor network that contributed to the overall device capacitance and effective dielectric constant. Again, various types of high magnification TEM were critical in resolving the Ti-based core-shell structure and its role in high-k behaviour. The vacuum-based alternate deposition technique has been developed to offer ease of operation, reliability, flexibility and applicability to chemically different filler and matrix systems in the fabrication of high-k PNC based capacitors, in which high-k performance relies critically on the formation of core (metal)-shell (oxide) particles in both Al and Ti based systems.