Our previous study identified one candidate transcript, chicken prostaglandin D2 synthase (cPGDS), significantly higher expressed in hens with high-egg production across several chicken strains including the Single-Comb White Leghorn layers. To further elucidate its underlying molecular mechanisms, the downstream effects of cPGDS in primary culture of chicken anterior pituitary cells were investigated. We successfully generated a highly specific rabbit anti-cPGDS polyclonal antibody. Of 13 examined tissues/cells, quantitative reverse transcription polymerase chain reaction and Western blotting analysis demonstrated that cPGDS mRNA and protein were highly expressed in intestine, kidney, liver and pituitary gland. In addition, significantly higher cPGDS mRNA and protein expression levels of the pituitary gland and ovary in hens than of the pituitary gland and testis in roosters with the same age were found. Transfection of pEGFP-cPGDS plasmid into primary culture of pituitary anterior cells in medium that contained 1 mM arachidonic acids induced luteinizing hormone beta subunit (LHB) transcription and subsequent translation. Treatments of two metabolites in the cyclooxygenase pathway, PGD2 and PGJ2 in the primary culture of chicken anterior pituitary cells induced LHB transcription and translation in both dose- and time-dependent manners. Treatment of peroxisome proliferator activated receptors (PPARs) agonist increased LHB transcription and translation in chicken anterior pituitary cells. On the other hand, specific inhibitions of PPARA and PPARG using antagonists dramatically suppressed PGJ2-induced LHB transcription level. Taken together; these data suggested PGDS upregulated LHB transcription in primary culture of chicken anterior pituitary cells via the PPAR signaling pathway.

This thesis compare stationary and nonstationary numeric model of conduction and convection of heat in sapwood part of tree. Numeric models are testing with diferent moistures of sapwod and diferent sapflows. There are tested HFD and LHB methods in models which are compared each other. LHB method has substantially better results. Stationary and nonstationary models are almost unchanged in decreasing sapflow. Divergency of individual models rises at increasing sapflow. There is nonstationary method departed from stationery method in the initial sapflow.

The Bogie frame is an important and integral member of bogie. In Indian Railways, LHB (Linke Hofmann Busch) coaches are used as passenger coaches. They are equipped with FIAT bogie frames. Inorder to overcome the limitations of the existing FIAT bogie frame structure, a new bogie frame structure namely New CASNUB Bogie Frame is designed to equip with LHB coach. The New CASNUB Bogie Frame design is validated by conducting Stress analysis using ANSYS Mechanical APDL software. The stresses induced in both the frame structures are compared. Stresses induced in the New CASNUB Bogie frame are lesser than in the FIAT Bogie frame and are within the allowable stress limits of material used. New CASNUB Bogie Frame can be used as an alternative for LHB coaches in Indian Railways.

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