Title from second page of PDF document. Includes bibliographical references.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-12-18T18:25:58Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariana_generoso_ferreira.pdf: 1487910 bytes, checksum: 5d8038ca69034b764ba796a3a18d0907 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-01-03T17:13:11Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariana_generoso_ferreira.pdf: 1487910 bytes, checksum: 5d8038ca69034b764ba796a3a18d0907 (MD5) / Made available in DSpace on 2018-01-03T17:13:11Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariana_generoso_ferreira.pdf: 1487910 bytes, checksum: 5d8038ca69034b764ba796a3a18d0907 (MD5) Previous issue date: 2017 / O agricultor deve adequar-se ?s exig?ncias da nova legisla??o ambiental brasileira, quanto ? recupera??o de ?reas de preserva??o permanente e reserva legal. Um dos problemas que o produtor pode enfrentar ? a recupera??o de ?reas degradadas, que podem conter, entre outros contaminantes, res?duos de herbicidas. O 2,4-D e picloram s?o herbicidas hormonais mimetizadores de auxina. Entre os grupos vegetais, as esp?cies das matas ciliares t?m sido pouco relatadas quanto aos efeitos de res?duos desses herbicidas no ambiente. As an?lises micromorfol?gicas e micromorfom?tricas s?o ferramentas importantes no aux?lio do diagn?stico precoce da inj?ria, visto que modifica??es vis?veis podem aparecer em fase tardia da intoxica??o. Objetivou-se com este trabalho avaliar o desenvolvimento inicial de dez esp?cies arb?reas em substrato com res?duos da mistura de herbicidas 2,4-D+picloram e verificar os efeitos nas modifica??es anat?micas foliares de Mabea fistulifera e Zeyheria tuberculosa. Em delineamento em blocos ao acaso com tr?s repeti??es, foram distribu?dos 40 tratamentos em esquema fatorial 4x10. O primeiro fator foi composto por quatro doses da mistura comercial de 2,4-D+picloram correspondentes a 0,00; 0,166; 0,333 e 0,666 g ha-1 (Tordon?, contendo 240 g de 2,4-D e 64 g de picloram por litro). O segundo fator foi composto por dez esp?cies arb?reas: Machaerium opacum Vogel, Machaerium nyctitans (Vell.) Benth., Cassia ferruginea (Schrad.) Schrad. ex DC., Senna macranthera (DC. ex. Collad.) H.S. Irwin e Barnaby, Anadenanthera colubrina (Vell.), Brenan, Senegalia polyphylla (DC.) Britton e Rose, Piptadenia gonoacantha (Mart.) J.F. Macbr., Dalbergia villosa (Benth.) Benth., Mabea fistulifera Mart. e Zeyheria tuberculosa (Vell) Bureau ex Verl. Avalia??es morfol?gicas e anat?micas em plantas jovens foram realizadas conforme a metodologia padr?o. Para as vari?veis morfol?gicas das dez esp?cies arb?reas foram avaliados: intoxica??o, sobreviv?ncia, altura das pl?ntulas, n?mero de folhas, ?rea foliar, massa seca da parte a?rea e da raiz, di?metro do caule, o volume da raiz, o ?ndice de velocidade de emerg?ncia e a emerg?ncia. Por meio de avalia??es micromorfom?tricas foram medidas, na sec??o transversal das folhas de Mabea fistulifera e Zeyheria tuberculosa, a espessura dos tecidos, a epiderme adaxial e abaxial, par?nquima pali??dico e lacunoso, al?m da l?mina total. Para a ?rea foliar foram realizadas fotografias das folhas e mensuradas com aux?lio do software Image K. As esp?cies arb?reas sobreviveram ? aplica??o do produto, com varia??o na sensibilidade. Os res?duos da mistura dos herbicidas prejudicaram a maioria das esp?cies testadas em rela??o ?s avalia??es do desenvolvimento inicial. M. fistulifera, P. gonoacantha e Z. tuberculosa apresentaram maior toler?ncia ? mistura de herbicidas. Em rela??o ? anatomia e ?rea foliar, de maneira geral, a esp?cie Z. tuberculosa foi afetada negativamente pelos herbicidas. M. fistulifera se mostrou mais tolerante ? presen?a dos contaminantes. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The farmer must adapt to the requirements of the new brazilian environmental legislation, regarding the recovery of permanent preservation areas and legal reserve. One of the problems that the producer may face is the recovery of degraded areas, which may contain, among other contaminants, herbicide residues. 2,4-D and picloram are auxin mimicking hormonal herbicides. Among the plant groups, the species of the riparian forests have been little reported as to the effects of residues of these herbicides on the environment. Micromorphological and micromorphometric analyzes are important tools to aid in the early diagnosis of injury, since visible changes may appear in the later stages of intoxication. The objective of this work was to evaluate the initial development of ten substrate tree species with residues from of the 2,4-D + picloram herbicide mixture and to verify the effects on the anatomical modifications of Mabea fistulifera and Zeyheria tuberculosa. In a randomized block design with three replicates, 40 treatments were distributed in a 4x10 factorial scheme. The first factor was composed of four doses of the commercial mixture of 2,4-D + picloram corresponding to 0,00; 0,166; 0,336 and 0,666 g ha-1 (Tordon?, containing 240 g of 2,4-D and 64 g of picloram per liter). The second factor was composed of ten tree species: Machaerium opacum Vogel, Machaerium nyctitans (Vell.) Benth., Cassia ferruginea (Schrad.) Schrad. ex DC., Senna macranthera (DC. ex. Collad.) H.S. Irwin e Barnaby, Anadenanthera colubrina (Vell.), Brenan, Senegalia polyphylla (DC.) Britton e Rose, Piptadenia gonoacantha (Mart.) J.F. Macbr., Dalbergia villosa (Benth.) Benth., Mabea fistulifera Mart. and Zeyheria tuberculosa (Vell) Bureau ex Verl. Morphological and anatomical evaluations in young plants were performed according to the standard methodology. For the morphological variables of the ten tree species were evaluated: intoxication, survival, seedling height, number of leaves, leaf area, dry mass of shoot and root, stem diameter, root volume, emergency speed index and emergency. Through micromorphometric evaluations, tissue thickness, adaxial and abaxial epidermis, pali?adic and lacunacetic parenchyma were measured, in the cross section of the leaves of Mabea fistulifera and Zeyheria tuberculosa, in addition to the total lamina. For leaf area, leaf photographs were taken and measured using Image K software. Tree species survived the application of the product, with variation in sensitivity. Residues of the herbicide mixture adversely affected most of the tested species in relation to initial developmental assessments. M. fistulifera, P. gonoacantha and Z. tuberculosa presented greater tolerance to the herbicide mixture. In relation to the anatomy and leaf area, in general, the species Z. tuberculosa was negatively affected by the herbicides. M. fistulifera was more tolerant to the presence of contaminants.

Mesophyll conductance is (together with stomatal conductance) a crucial component of diffusion limitations of photosynthesis and it is important to understand the mechanisms of CO2 fluxes through the leaves. Here I tested a new technique for estimation of drawdown in CO2 concentration across hypostomatous leaves based on carbon isotope composition (13C) of leaf cuticle and cuticular waxes isolated from opposite leaf sides.

Orientador: Tatiane Maria Rodrigues / Resumo: Em folhas de diversas espécies vegetais, a bainha de células que envolve os feixes vasculares se estende em direção à epiderme em ambas as faces do limbo formando extensões da bainha do feixe (EBF), o que caracteriza folhas heterobáricas. Folhas que não apresentam EBF são chamadas homobáricas. Pesquisas demostram que folhas homobáricas garantem maior difusão lateral de gases, enquanto as EBF podem atuar na condução de água e aumentar o desempenho fotossintético foliar garantindo a transferência de luz para as camadas mais internas do mesofilo. As diferenças morfofuncionais entre os dois tipos foliares parecem estar relacionadas ao ambiente onde as plantas ocorrem e à sua distribuição nos diferentes estratos vegetacionais. Entretanto, para formações vegetais no Brasil não foram encontrados estudos sobre a distribuição de espécies com folhas homobáricas e heterobáricas. Esse trabalho teve como objetivo estudar a anatomia foliar de espécies herbáceas, arbustivas e arbóreas na floresta estacional semidecídua, cerradão e cerrado stricto sensu a fim de identificar folhas homobáricas e heterobáricas, buscando associar a presença/ausência de EBF com o porte das plantas e com o ambiente. Quadrantes de 20mX20m foram demarcados nas fragmentos vegetacionais. Folhas completamente expandidas foram coletadas de 59 espécies no cerradão (32 arbóreas; 19 arbustos e 8 herbáceas); 68 espécies no cerrado stricto sensu (21 arbóreas; 30 arbustos e 17 herbáceas) e 51 espécies na floresta estacional ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: In leaves of several plants species, the vascular bundle sheath extends toward the epidermis on both sides of the blade forming bundle sheath extensions (BSE), characterizing heterobarics leaves. Leaves lacking BSE are called homobarics. Research shows that homobaric leaves provide greater lateral movements of gases, while the BSE can act conducting water and increasing the leaf photosynthetic performance, what ensures the transference of light to the inner layers of the mesophyll. The morphological and functional differences between the two leaf types seem to be related to the environment where the plants occur and their distribution in the different vegetation strata. However, there are no studies on the distribution of species with homobarics and heterobarics leaves for vegetation formations in Brazil. This work aimed to study the leaf anatomy of herbs, shrubs and trees in semideciduous seasonal forest, cerradão and cerrado stricto sensu to identify homobarics and heterobarics leaves, searching to associate the presence / absence of BSE to the plant strata and environmental characteristics. Quadrants of 20mX20m were marked in vegetation fragments. Fully expanded leaves were collected from 59 species in the cerradão (32 tree, 19 shrubs and 8 herbs); 68 species in the cerrado stricto sensu (21 tree, 30 shrubs and 17 herbs) and 51 species in the semideciduous seasonal forest (23 tree, 17 shrubs and 11 herbs). Samples of the leaf blade were processed according to standard tech... (Complete abstract click electronic access below) / Mestre

CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Melastomataceae Juss, a maior família da ordem Myrtales, apresenta ampla distribuição pantropical. A família é uma das maiores da flora brasileira, somando cerca de 150 gêneros e 4.500 espécies. Melastomataceae é dividida em 11 tribos, sendo Melastomeae Bartl., Miconieae DC. e Microlicieae Naudin as tribos mais importantes para o Brasil, onde podem ser encontradas cerca de 1/3 de suas espécies. Microlicia D. Don, o maior gênero da tribo Microliciae, é um dos gêneros mais representativos, com cerca de 120 espécies, concentradas principalmente nos campos rupestres do Brasil Central. Embora atinjam sua maior diversidade nos campos rupestres, algumas espécies de Microlicia têm ampla distribuição, podendo ser encontradas em outras fitofisionomias, como cerrado, veredas, campos úmidos e campos hidromórficos. Microlicia é considerado um gênero problemático entre as Melastomataceae, sendo suas espécies reconhecidas apenas pela combinação de diferentes características, o que torna difícil a identificação precisa de várias delas. Numa tentativa de elucidar a delimitação dos taxa mais problemáticos, um grande esforço tem sido dedicado ao estudo da anatomia foliar de espécies do gênero na última década, e hoje cerca de 1/3 das espécies já tiveram a anatomia foliar descrita. As características mais comuns para o gênero são: epiderme uniestratificada, folhas anfiestomáticas, presença de apêndices epidérmicos variados, mesofilo isobilateral ou homogêneo, e presença de compostos fenólicos nos tecidos da folha, principalmente nas células do parênquima paliçádico. No entanto, apesar das várias características em comum, as folhas de Microlicia exibem também características anatômicas diferentes, que poderiam auxiliar na identificação dos taxa de reconhecimento mais impreciso. Considerando o grande número de espécies de Microlicia com a anatomia foliar descrita, o polimorfismo relatado para várias espécies do gênero e o possível potencial plástico da folha, que as espécies de ampla ocorrência poderiam apresentar, o presente estudo teve como objetivos gerais: a) descrever a anatomia foliar de mais nove espécies do gênero, ocorrentes nos campos rupestres; b) descrever a anatomia foliar de quatro espécies ocorrentes em diferentes ambientes, além dos campos rupestres e c) através da análise de similaridade, realizada com base nas características anatômicas da folha de todas as Microlicia estudadas até o momento, analisar os grupos de espécies semelhantes formados e identificar caracteres que propiciem a formação dos mesmos. / Melastomataceae Juss, the largest family of the order Myrtales, is widely distributed pantropical. The family is one of the largest of the Brazilian flora, with about 150 genera and 4,500 species. Melastomataceae is divided into 11 tribes, with Melastomeae Bartl., Miconieae DC. and Microlicieae Naudin the most important tribes in Brazil, and in Brazil can be found about 1/3 of its species. Microlicia D. Don the largest genus of Microliciae tribe and one of the most representative genera, with about 120 species, mainly concentrated to the “campos rupestres” in the Central Brazil. Although Microlicia reaching its greatest diversity in the Brazilian “campos rupestres”, some species are widely distributed and can be found in other vegetation types such as cerrado, paths, swamps and hydromorphic fields. Microlicia is considered a problematic genus among the Melastomataceae, and their species are recognized only by combining different traits, making it difficult to accurately identify several of them. In an attempt to help the delimitation of the most problematic species, in the last decade a great effort has been devoted to the study of Microlicia leaf anatomy and today around 1/3 of these species have had the leaf anatomy described. The most common features are: unisseriate epidermis, amphistomatic leaves, presence of trichomes and emergences, isobilateral or homogeneous mesophyll and presence of phenolic compounds in leaf tissues, particularly in the palisade parenchyma cells. However, despite the many similar features, Microlicia leaves also exhibit different anatomical characteristics that could help identify the most problematic species. Considering the large number of Microlicia species with the leaf anatomy known, the polymorphism already reported for several species of the genus, and the possible plastic potential of the leaf anatomy that the species of widely spread could present, the goals of this work were: a) analyze the leaf anatomy of nine Microlicia from “campos rupestres”; b) analyze and interpret the leaf anatomy of four species occurring in others environments, beyond the “campos rupestres” and c) through similarity analysis performed using the anatomical leaf traits of the all Microlia studied to date, analyze the groups of similar species formed and identify characters that favor the formation of the same. / Dissertação (Mestrado)

Made available in DSpace on 2014-08-20T13:59:16Z (GMT). No. of bitstreams: 1 dissertacao_anderson_schock.pdf: 1206821 bytes, checksum: 4f2fc22ec8bca43a2893fc6979ea3727 (MD5) Previous issue date: 2012-02-27 / Among the many species of plants oleaginous plants that have been studied under the proposal to produce biodiesel, the physics nut (Jatropha curcas L.) has emerged due to large percentage of oil contained in seeds and its adaptive capacity, the soil and... / Dentre as muitas espécies de plantas oleaginosas estudadas sob a proposta de produção de biodiesel, a Jatropha curcas L. tem se destacado em função da grande porcentagem de óleo contido nas sementes e de sua capacidade adaptativa...

Contexte et objectifs : Les tentatives pour expliquer les variations des paramètres foliaires fondées uniquement sur les facteurs environnementaux, ce qui conduira à des erreurs importantes si la plante montre des variations ontogénétiques substantielles des propriétés foliaires. Nous avons évalué les variations des 27 traits morpho-anatomiques des feuilles de Dipterocarpus alatus durant six stades de développement architectural et entre les axes à un stade de développement donné sous deux conditions lumineuses différentes.Méthodologies:Une analyse architecturale a été réalisée pour distinguer précisément et objectivement des catégories d'axes et stades de développement de Dipterocarpus alatus. Les feuilles ont été collectées sur l'unité de croissance plus récente et complète sur la partie supérieure du tronc et sur la partie plus externe de la branche du milieu couronne des arbres qui poussent sous deux différentes conditions lumineuses. Vingt-deux traits foliaires ont été mesurées et calculées sur des images de tracés feuilles et de sections transversaux à l'aide de logiciel ImageJ. Densité stomatal a été calculée sur l'impression de vernis à ongles de la surface inférieure des feuilles. Quatre traits de chlorophylle ont été déterminées. Les tests d'ANOVA et Tukey ont été utilisés pour déterminer les différences entre les stades de développement et des axes. La contribution relative lmg a été calculée avec package R relaimpo et comparées avec l'analyse bootstrap pour déterminer le stade ontogénétique, catégorie axe ou l'intensité lumineuse, ce qui est expliqué plus les variations de l'anatomie des feuilles.Résultats:La morphologie et l'anatomie des feuilles diffère fortement durant l'ontogenèse pour les quatre axes en terme d'ordre et de catégorie deux sous des conditions lumineuses. L'effet de l'axe a été présentée au stade B, C, D et F, mais ont tendance à être insignifiante au stade E. Ontogenèse de l'arbre expliqué plus de variations dans la morphologie des feuilles et de l'anatomie que l'intensité lumineuse. Catégorie d'axe expliqué plus de variations dans la morphologie des feuilles, mais pas dans l'anatomie des feuilles que l'ontogenèse de l'arbre. Conclusions:Fortes et significatives variations intra-spécifiques (pendant l'ontogenèse et entre axes) peuvent influencer les variations inter-spécifiques, et donc contestent la validité de la valeur moyenne des traits foliaires entre les espèces. Ontogenèse de l'arbre contribué plus que l'intensité de la lumière dans l'explication de la variabilité dans la morphologie et l'anatomie des feuilles à la fois sur le tronc et deuxième l'ordre l'axe recommande fortement que les études sur les réponses de la morphologie et l'anatomie des feuilles à l'environnement doivent corriger l'effet de l'ontogenèse. / Background and Aims:Attempts to explain variations in leaf parameters based solely on environmental factors, this will lead to significant errors if the plant shows substantial ontogenetic variations in leaf properties. We evaluated variations in 27 morpho-anatomical leaf traits of Dipterocarpus alatus over six architectural development stages and between axes at a given development stage under two different light conditions. Methods:An architectural analysis was conducted to distinguish precisely and objectively axis categories and developmental stages of Dipterocarpus alatus. Leaves were collected on the most recent complete growth unit on the top of the trunk and the outermost part of the middle-crown branch with branchlet and twig from trees growing under two different light conditions. Twenty two leaf traits were measured and calculated using ImageJ on images of leaf tracings and cross-sections. Stomatal density was calculated on nail varnish impression of the leaf lower surface. Four chlorophyll traits were determined. One-way ANOVA and Tukey post-hoc tests were used to determine differences between development stages and axes. The lmg relative contribution was calculated with R package relaimpo and compared with bootstrap analysis to determine ontogenetic stage, axis category or light intensity, which explained more the variations in leaf anatomy.Key results:Leaf morphology and anatomy differed strongly during ontogeny for all four axes in term of order and category under both light conditions. The axis effect was displayed at stages B, C, D and F but tend to be insignificant at stage E. Tree ontogeny explained more variations in leaf morphology and anatomy than light intensity. Axis category explained more variations in leaf morphology but not in leaf anatomy than tree ontogeny. Conclusions: Strong and significant intraspecific variations (during ontogeny and between axes) may influence the interspecific variations, and thus challenge the validity of the mean value of leaf traits between species. Tree ontogeny contributed more than light intensity in explanation of the variability in leaf morphology and anatomy both on the trunk and second axis order strongly recommends that studies on the responses of leaf anatomy to the environment need to correct for the ontogeny effect.

Submitted by Rafael Santana (rafael.silvasantana@ufpe.br) on 2017-06-09T18:33:27Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Tese Bárbara Laine Ribeiro da Silva.pdf: 2635614 bytes, checksum: 8384212e8db84a27bc2b1fd6a579e7ad (MD5) / Made available in DSpace on 2017-06-09T18:33:27Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Tese Bárbara Laine Ribeiro da Silva.pdf: 2635614 bytes, checksum: 8384212e8db84a27bc2b1fd6a579e7ad (MD5) Previous issue date: 2016-04-01 / CAPES / Em ambientes com clima semiárido, a sobrevivência das plantas depende da adaptação de características morfológicas, anatômicas e fisiológicas. O hábito das espécies e a estrutura do ambiente no qual a planta está inserida também determina sua sobrevivência em ambientes com condições ambientais limitantes, como a caatinga. No caso das espécies herbáceas, é necessário descobrir qual a principal limitação ambiental para o seu desenvolvimento, já que a maioria delas possui ciclo de vida restrito ao período de chuvas na região, bem como quais são as características desenvolvidas que permitem sua sobrevivência no ambiente. O trabalho avaliou características fisiológicas, anatômicas e morfológicas de três espécies herbáceas (Hyptis suaveolens L., Stachytarpheta sanguinea Mart. ex Schauer e Jacquemontia evolvuloides (Moric.) Meisn., em áreas de caatinga com 22, 44 e mais de 60 anos de regeneração, na Paraíba. Foram analisadas: área foliar; área foliar específica; espessura das epidermes, das cutículas, dos parênquimas e do mesofilo; densidade estomática e de tricomas; concentrações de clorofila a, clorofila b e carotenoides; potencial hídrico foliar, trocas gasosas, concentrações de compostos fenólicos totais, nitrogênio, potássio e fósforo. As três espécies apresentaram características anatômicas de folhas de sol, com estômatos e tricomas em ambas as faces foliares e maior concentração na face abaxial. J. evolvuloides apresentou mais características de locais ensolarados, com a menor área foliar específica na área em regeneração mais recente, maior espessura de epiderme e parênquima isobilateral. Algumas variações morfoanatômicas entre as áreas foram comuns entre as três espécies, como as maiores espessuras dos mesofilos e dos parênquimas na caatinga mais antiga que nas áreas com 22 e 44 anos em regeneração. No geral, H. suavoelens e S. sanguinea foram espécies mais plásticas que J. evolvuloides, com as maiores diferenças morfofisiológicas entre as áreas. J. evolvuloides demonstrou ser mais adaptada à baixa disponibilidade hídrica e à alta incidência luminosa. / In environments with semi-arid climate, the survival of plants depends on the adaptation of morphological, anatomical and physiological characteristics. The habit of the species and environmental structure in which the plant is inserted, also determines their survival in environments with limiting environmental conditions, such as the caatinga. In the case of herbaceous species, it is necessary to find out what is the main environmental limitations for its development, since most they have a life cycle restricted to the rainy season in the region, and what are the features developed to survival in the environment. This work determined the physiological, anatomical and morphological characteristics of three herbaceous species (Hyptis suaveolens L. Stachytarpheta sanguinea Mart. Ex Schauer and Jacquemontia evolvuloides (Moric.) Meisn., in areas of caatinga with 22, 44 and over 60 years of regeneration, in Paraiba state. Were analyzed the leaf area, specific leaf area, thickness of the epidermis, cuticle, parenchyma and mesophyll, the stomata and trichomes densities; concentrations of chlorophyll a, chlorophyll b and carotenoids, the leaf water potential, gas exchange, concentrations of total phenolic compounds, nitrogen, potassium and phosphorus. The three species have anatomical features of sun leaves with stomata and trichomes in both leaf surfaces and a greater concentration on the abaxial surface. J. evolvuloides showed more characteristics of sunny locations, with lowest specific leaf area in the most recent regeneration area, thickest epidermis and isobilateral parenchyma. Some morphoanatomical variations between the areas were common among the three species, such as the greater thicknesses of mesophyll and parenchyma in the oldest caatinga than in the areas with 22 and 44 years in regeneration. Overall, H. suavoelens and S. sanguinea were more plastic species than J. evolvuloides, with the largest morphophysiological differences between areas. J. evolvuloides proved to be more acclimatized to low water availability and high light incidence.

The present Master thesis focuses on evalution of CO2 concentration and irradiance on selected leaf anatomical parameters of European Beech (Common Beech) Fagus sylvatica L.. The process of photosynthesis is remarkably determined by numerous extrenal factors, among them by atmoshperic CO2 concentration and irradiance and is closely correlated with leaf anatomical parameters. One of these most important anatomical parameters affecting the net assimilation rate is an internal leaf surface, which corresponds to mesophyll area avialable for gas exhcange. Experimental material of the study was sampled from the leaves of juvenile trees of F. sylvatica planted in 2005 and growing under ambient (390 ppm, AC) and elevated (700 ppm, EC) CO2 concentrations on the experimental site of the Global Change Research Center AS CR at Bílý Kříž in the Beskydy Mountains. Sun and shade leaves were sampled from trees of both CO2 treatments in two seasons 3 years apart (2009 and 2012). To determine leaf anatomical parameters, the stereological methods were applied, which yield unbiased estimation of measured parameters, particularly the Fakir method for internal leaf surface determination. The EC effect was observed on the leaves sampled in 2009 only in the decrease of proportion of intercellular spaces in mesophyll. In...

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