Thesis (Ph.D.) -- University of Texas Southwestern Medical Center at Dallas, 2005. / Not embargoed. Vita. Bibliography: 114-132.

Techn. University, Diss., 2005--München.

Thesis (Ph. D.)--Illinois State University, 2004. / Title from title page screen, viewed May 23, 2006. Dissertation Committee: Brian J. Wilkinson (chair), Radheshyam K. Jayaswal, Anthony J. Otsuka, David L. Williams, Wade A. Nichols. Includes bibliographical references (leaves 105-113) and abstract. Also available in print.

Würzburg, Univ., Diss., 1999. / Dateiformat: zip, Dateien in unterschiedlichen Formaten

München, Techn. Univ., Diss., 2000.

Thesis (M.S.)--Oregon State University, 2008. / Printout. Includes bibliographical references. Also available on the World Wide Web.

Publicación a texto completo no autorizada por el autor / Evalúa la capacidad de formación de biopelículas de 20 cepas de Listeria monocytogenes aisladas de embutidos y superficies inertes de una planta de procesado cárnico. Se evalúan diferentes condiciones de temperatura: refrigeración (4ºC), ambiente (22ºC) y 35ºC, obteniendo formación de biopelículas en el 95% de las cepas según la clasificación Stepanovic. El 85% son productoras débiles de biopelículas a 4°C y 35°C; sin embargo a 35°C un 10% son productoras moderadas. El uso de un desinfectante eficiente y la concentración adecuada para reducir la formación de las biopelículas es de suma importancia. Para ello se analiza la acción de dos desinfectantes a base de Hipoclorito de sodio y Amonio cuaternario a tres tiempos de contacto (5, 10 y 30 minutos). Los resultados del presente estudio demuestran que el hipoclorito de sodio es más eficiente para eliminar las biopelículas que el amonio cuaternario a partir de los 30 minutos de exposición. / Tesis

Master of Agribusiness / Department of Agricultural Economics / Keith D. Harris / Food processing facilities are faced with many challenges in ensuring that the food supply is safe for consumption. Listeria monocytogenes is a food pathogen that has been linked to ready-to-eat foods, including tree nuts. Listeria monocytogenes is part of the ubiquitous microorganism genus, Listeria. The most likely cause of Listeria contamination in food is post-processing contamination. The purpose of this research is to identify and examine possible solutions a nut processing facility might employ to mitigate a food safety risk. The outcome of this research helps to establish the most financially viable method a processing facility may implement to address and mitigate an established risk given defined premise construction and constraints. The research objective is to identify a solution, implement a course of action, and establish safeguards to prevent recurrence of the issue. Factoring in facility specific variables as well as industry data and relevant analyses, the research conducted concludes with recommended actions for the facility to make, including a combination of structural design changes coupled with extensive chemical sanitation techniques.

Nous avons montré que, dans l'intestin, les cellules exprimant le marqueur spécifique des lymphocytes Natural Killer (NK), NKp46 peuvent être divisées en deux compartiments, par rapport au facteur de transcription RORyt. Les cellules NKp46+RORyt- sont des cellules NK intestinales immatures. Cependant, les cellules NKp46+RORyt- s'apparentent aux cellules "lymphoid tissue-inducer" (LTi), puisqu'elles requièrent RORyt pour leur développement et produisent de l'IL-22, cytokine essentielle pour l'homéostasie et la réponse antimicrobienne épithéliale. Notre recherche actuelle vise à établir les relations ontogéniques et la signature génétique de ces cellules au niveau pan-génomique. La fonction in vivo des cellules NKp46+ intestinales est adressée dans un modèle unique d'infection par voie orale avec Listeria monocytogenes. L'ensemble de nos résultats a montré que les cellules NKp46+ ont une place légitime dans la famille émergente des cellules intestinales de l'immunité innée. / Natural Killer (NK) cells are NKp46+CD3- innate lymphocytes, which exhibit cytotoxicityand cytokine production, mainly IFN-γ, as major effector functions. In mammals, theexpression of natural cytotoxic receptor NKp46 is essentially restricted to NK cellcompartment. However, we showed that in mouse intestine, NKp46 marker defines aheterogeneous cell population, differentially expressing the retinoic acid orphan receptorRORγt. NKp46+RORγt- cells harbor reduced cytotoxicity and IFN-γ production, consistentwith an immature NK cell phenotype. In contrast, NKp46+RORγt+ cells resemble lymphoidtissue inducer cells (LTi) in their developmental requirement for RORγt and their ability toproduce IL-22. This cytokine is critical for epithelial homeostasis and antimicrobial responseat several epithelial sites.By using a genome wide profiling approach, we confirmed that gut NKp46+RORγt- cellsare the only gut NKp46+ cell population related to conventional splenic NK cells, while gutNKp46+RORγt+ cells are linked to LTi cells. Transcriptional signatures specific of distinct gutNKp46+ cell subsets are currently under intensive investigation in order to determine novelfunctional properties.We assessed gut NKp46+ cell subsets in vivo function during oral infection with entericpathogen Listeria monocytogenes (L.m.). Although immune responses to systemic L.minfection have been widely characterized in mice, L.m. cannot breach mouse intestinal barrier,thus limiting the knowledge of early immune responses at the natural site of infection. Using aunique transgenic mouse lineage restoring L.m. intestinal invasiveness, we showed that gutNKp46+RORγt- and NKp46+RORγ t+ cells respond to L.m. infection by producing IFN-γ andIL-22, respectively. Further investigation of the cellular mechanisms leading to gut NKp46+cell subset activation, as well as the contribution of these cells to the control of bacterialdissemination is in progress.Altogether our data provide novel insight into intestinal innate immunity and highlight gutNKp46+ cell subsets as “legitimate” members of the emerging family of intestinal innatelymphoid cells

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-10-26T12:10:08Z No. of bitstreams: 2 aline_silva_santos.PDF: 1329305 bytes, checksum: 5deee2946b99b6eac0e5c8cc5c537c15 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-10-26T12:11:13Z (GMT) No. of bitstreams: 2 aline_silva_santos.PDF: 1329305 bytes, checksum: 5deee2946b99b6eac0e5c8cc5c537c15 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2015-10-26T12:11:13Z (GMT). No. of bitstreams: 2 aline_silva_santos.PDF: 1329305 bytes, checksum: 5deee2946b99b6eac0e5c8cc5c537c15 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2010 / O objetivo deste trabalho foi verificar a qualidade microbiol?gica do leite cru e da ?gua utilizados na fabrica??o do queijo e a qualidade microbiol?gica do queijo Minas Artesanal do Serro em temperatura ambiente e sob refrigera??o (8oC), durante 63 dias de matura??o, e sua compara??o com os crit?rios microbiol?gicos exigidos pela Lei Estadual n.? 14.185, de 31 de janeiro de 2002, espec?fica para queijos artesanais. Todas as amostras de ?gua apresentaram contamina??o por coliformes totais acima do valor m?ximo permitido. Nenhuma amostra de ?gua apresentou contamina??o por coliformes fecais. Para duas amostras de leite cru a contagem de mes?filos apresentou-se acima dos limites permitidos pela legisla??o brasileira. As contagens de Staphylococcus aureus no leite variaram entre 0 log UFC/mL e 3,46 log UFC/mL. Escherichia coli esteve ausente em todas as amostras de leite analisadas. A contagem de c?lulas som?ticas variou entre 13.500 c?lulas/mL e 260.500 c?lulas/mL e somente uma amostra estava fora dos padr?es para acidez. Listeria monocytogenes e Salmonella spp n?o foram encontradas em nenhuma das amostras de queijo analisadas nos diferentes tempos e temperaturas de matura??o e n?o houve efeito significativo das condi??es de matura??o em seus par?metros microbiol?gicos. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010. / ABSTRACT The aim of this study was to assess the microbiological quality of raw milk and water used in cheese production and the microbiological quality of handmade Minas Cheese produced in Serro-MG at room temperature and under refrigeration (8?C) during 63 days of maturation and its comparison with the microbiological criteria required by the State Law N? 14,185 of January 31, 2002, specifically to handmade cheeses. All of the water samples showed total coliform count above the maximum allowed. No water sample showed contamination by fecal coliforms. For two raw milk samples the mesophilic count presented above the allowed limits according to the Brazilian law. Staphylococcus aureus counts ranged from 0 log CFU/mL and 3.46 log CFU/mL. No Escherichia coli was observed in the analyzed milk samples. The counted somatic cell ranged from 13,500 cells/mL to 260,500 cells/mL and only one sample was out of the standards for acidity. No Listeria monocytogenes or Salmonella spp were found in the analyzed cheese samples at different times and temperatures of maturation and there were no significant effect of maturation conditions on their microbiological parameters.