Cette thèse consiste en une analyse de l’État sénégalais en actes et de ses processus de gouvernementalité tels que ceux-ci sont déployés en Casamance. Au travers de la lutte contre le paludisme, comme objet ethnographique, ce travail mène une analyse de la fabrique de la légitimité étatique dans une région où celle-ci est remise en question. Il s'agit, au travers des actes d’État, d'interroger les relations entre les appareils d’État (idéologiques et coercitifs), les partenaires de l’État et les communautés. Les ajustements structuraux des années 1980, la décentralisation qui en découle et la mise en place des appareils communautaires ont participé, auprès de la population, de la dégradation de l'image d'un État fort. Cette dégradation, en contexte casamançais, fut exacerbée par une crise de la légitimité étatique trouvant son origine dans un conflit social. Celui-ci est survenu suite au sentiment éprouvé par les populations d'un État vécu comme pilleur et incapable d'assurer le bien-être de ses administrés. Aujourd'hui, alors que la Casamance reste en proie à un conflit de basse-intensité, l'imaginaire d'un État ayant « abandonné » la région subsiste. Comment, dès lors, l’État fabrique sa légitimité alors qu'il n'apparaît majoritairement aux yeux des Casamançais qu'au travers de ses prérogatives violentes ? Alors que ses appareils coercitifs sont très visibles (contrôles militaires et policiers réguliers), comment apparaît-il sous ses formes bienveillantes lui permettant de légitimer sa présence ? Ainsi, par une analyse du déploiement du biopouvoir, au travers de la mise en place des politiques de lutte contre le paludisme, cette thèse interroge la fabrique de l’État en Casamance. Cette recherche constitue ainsi une anthropologie politique. / This thesis consists in a Senegalese State analysis in its works and its governmentality process deployed in Casamance. Through the combat against malaria as an ethnographic field of investigation, this work analyses the fabrication of the State legitimacy in a region where it is challenged. It's about the investigation into the relations between the political apparatus (ideological and coercive), the government collaborators and communities through the State works. The structural adjustment of the 80's which has led to the decentralization and the community apparatus setting up, contributed to deteriorate the State's image for the people. A crisis of the legitimacy of the State rooted in a social conflict, exacerbated this deterioration in a Casamance context. This conflict appeared because the people felt that the State was a looter and was unable to provide for their well-being. Today, the imaginary of a State which has "given up" the region still goes on when Casamance still knows a weakened conflict. Consequently, how does the State build its legitimacy when it mainly reveals itself to the Casamances through its violent prerogatives? How does it appear by its benevolent forms allowing to legitimate its presence when its coercive apparatus are so obvious (military control and legitimate police)? Thus, this thesis questions, through an analysis of the deployment of the bio-powerand through the setting up of the policies to combat malaria, the fabrication of the State in Casamance. This research constitutes in this way a political anthropology.

Syfte och frågeställningar: Syftet är att studera om träning på olika intensitetsnivåer påverkar upplevd stress hos kvinnor i Sverige. Denna studie kommer svara på dessa frågeställningar:  <ol type="1">Finns det någon skillnad i upplevd stress hos kvinnor som deltar på lågintensiv träning i form av promenader jämfört med kvinnor som deltar på högintensiv träning i form av löpning? Finns det någon skillnad i upplevd stress hos kvinnor som deltar på högintensiv träning i form av löpning jämfört med kvinnor som inte tränar alls? Finns det någon skillnad i upplevd stress hos kvinnor som deltar på lågintensiv träning i form av promenader jämfört med kvinnor som inte tränar alls?           Metod: Studien är en tvärsnittsstudie, genom enkätundersökningar har skillnader i upplevd stress jämförts på kvinnor med medelålder 39,03 år ( ± 14,44), som antingen tränat högintensivt eller lågintensivt. Resultaten har jämförts med en referensgrupp bestående av kvinnor som inte tränar alls. Perceived Stress Scale-10 (PSS10) inklusive tio stycken intensitets-frågor för inklusion i studien skickades ut via sociala medier, Facebook. Resultat: En signifikant skillnad sågs vid jämförandet av kvinnor som tränar högintensivt och referensgrupp, (p=0,001), poängmässig skillnad i PSS10; 7,67 poäng. Ingen signifikant skillnad redovisades mellan högintensiv och lågintensiv grupp (p=0,737), detsamma gällde lågintensiv och referensgrupp (p=0,081). Slutsats: Högintensiva kvinnor hade reducerad upplevd stress jämfört med kvinnor som inte tränade alls. Ingen skillnad i upplevd stress kunde urskiljas mellan kvinnor som tränar lågintensivt och kvinnor som tränade högintensivt. Samma gällde kvinnor som tränade på låg intensitet jämfört med kvinnor som inte tränade alls, ingen skillnad i upplevd stress. / Purpose and aims: The aim is to study if different exercise-intensities affects perceived stress in Swedish women. This study will answer the following questions: <ol type="1">Is there a difference in perceived stress in women who participate in low-intensity exercise in the form of walking compared to women who participate in high-intensity exercise in the form of running? Is there a difference in perceived stress in women who participate in high-intensity exercise in the form of running compared to women who do not exercise at all? Is there a difference in perceived stress in women who participate in low-intensity exercise in the form walking compared to women who do not exercise at all?           Method: The study is a cross-sectional study, through questionnaires, differences in perceived stress have been compared in women with a mean age of 39,03 years (± 14,44), who either exercised with high intensity or with low intensity. The results have been compared to a reference group consisting of non-training women. Perceived Stress Scale-10 (PSS10) together with ten intensity-questions for inclusion in the study was sent out via social media, Facebook. Results: A significant difference was detected in the comparison of the high intensity group and the reference group, (p=0.001), score difference in PSS10; 7,67 points. No significant difference between the high-intensity group and the low-intensity group (p=0.737), the same applied to the low-intensity group and the reference group (p=0,081). Conclusions: Women who participated in high intensity exercise had reduced percieved stress compared to women who did not exercise at all. No difference in percieved stress was observed between women who participated in low intensity exercise and women who participated in high intensity exercise. Similarly, no difference in percieved stress in women who participated in low intensity exercise and women who did not exercise at all.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

This dissertation focuses on the sub-national impact of foreign aid on civil conflicts by asking the question: How does foreign aid committed to contested areas affect the intensity of violence in those areas? The main theoretical contribution is to focus on how aid influences warring parties’ decisions to engage in contests over territorial control and how that in turn influences violence intensity. The study introduces two concepts: funding concentration and barriers to exploiting aid. A contested area has greater concentration of funding if warring parties expect a high value of aid to be distributed to only a few locations. Funding is instead diffused if the parties expect aid to be spread over many locations. A low barrier to exploiting aid is present if it is of a type that both state and non-state actors could potentially misuse. There is a high barrier if territorial control is required in order to exploit funding channels. The theory introduces three testable implications: First, greater funding concentration encourages conventional contests over territorial control, which increases military fatalities. The second proposal is that if there is a low barrier to exploiting aid (e.g. humanitarian and food aid) then there will be increased competition between warring parties and civilians, and hence more civilian fatalities. Third, high barrier funding (e.g. education aid) will motivate contests over territorial control and increase military fatalities. This dissertation uses geo-coded aid commitments data and introduces data of warring parties’ battleground control in sub-Saharan Africa, 1989–2008. The research design relies on propensity score matching where pairs of observations are matched based on a range of covariates. The results concerning barriers to exploitation are partially supported. High barrier aid increases military fatalities whereas low barrier aid has little impact on violence. Greater funding concentration increases military fatalities substantially compared to if there is low or no funding concentration. In line with theory, greater funding concentration does not increase civilian fatalities.

As úlceras venosas representam um grande problema de saúde mundial e elevados custos associados ao tratamento a longo prazo. A terapia ultrassônica tem sido aplicada por décadas a fim de acelerar o processo de cicatrização de feridas crônicas. Muitos autores observaram os efeitos do ultrassom pulsado de baixa intensidade (LIPUS) em úlceras venosas, no entanto não há dados concisos sobre os modos de aplicação. O objetivo deste estudo foi avaliar a eficácia de duas técnicas de aplicação (borda e leito) do LIPUS, comparada à terapia convencional (creme sulfadiazina de prata 1%) na cicatrização de úlceras venosas crônicas. Para tanto, vinte e quatro pacientes com úlceras venosas foram avaliados no Ambulatório de Úlceras da Dermatologia (AUD) do Centro Saúde Escola (CSE) da Faculdade de Medicina de Ribeirão Preto (FMRP) da Universidade de São Paulo (USP) e alocados aleatoriamente nos: Grupo Borda (média da idade 63 anos; 37,5% mulheres; e 62,5% brancas) com 9 úlceras (13,49 \'+ OU -\' 14,41 \'CM POT.2\' inicial) tratadas com LIPUS (1,5 MHz, 30 mW/\'CM POT.2\') na borda; Grupo Leito (média da idade 73 anos; 100% mulheres; e 87,5% brancas) com 8 úlceras (15.61 \'+ OU -\' 13.36 \'CM POT.2\' inicial) tratadas com LIPUS no leito; e Grupo Sulfadiazina (média da idade 76 anos; 20% mulheres; e 40% brancas) com 7 úlceras (24,06 \'+ OU -\' 30,10 \'CM POT.2\' inicial) tratadas diariamente com creme sulfadiazina de prata 1%. Durante o tratamento, as úlceras foram fotografadas quinzenalmente e analisadas pelo software ImageJ a fim de se obter a área ulcerada e posterior Índice de Cicatrização da Úlcera (ICU) e Relação Esfácelo/Granulação (REG). No Grupo Leito foi realizada uma análise histopatológica (contagem de fibroblastos, células inflamatórias, vasos sanguíneos e colágeno) a partir de biópsias coletadas no 1º e no 45º dia de tratamento. Os dados foram analisados pelo teste de efeitos mistos e por Poisson a fim de comparar os grupos, com um nível de significância de 95% (p < 0,05). Assim, considerando as discretas diferenças encontradas no ICU e na REG dos dois grupos irradiados somadas às alterações histopatológicas do GL, torna-se evidente a ação positiva do LIPUS no tratamento das úlceras venosas crônicas superior à terapia com sulfadiazina de prata 1%, independente do local de aplicação. / Venous ulcers represent a major health problem in the world and high costs associated with long-term treatment. Ultrasonic therapy has been applied for decades to enhance the process of chronics wound healing. Many authors have assessed the effects of LIPUS on venous ulcers, but there were no data about the different application techniques. This study aimed to assess the effectiveness of two application techniques (edge and bed) of LIPUS, comparing with the conventional therapy (1% silver sulfadiazine cream) on the chronic venous ulcers healing. Therefore, twenty four patients with venous ulcers were evaluated at the Ulcers Outpatient of Dermatology at the Teaching Health Center, Faculty of Medicine of Ribeirão Preto - University of São Paulo (FMRP-USP) and randomly allocated, as follows: Edge Group (mean age 62.9 years, 37.5% women, 62.5% white) with nine ulcers (13,49 \'+ OR -\' 14,41 \'CM POT.2\') treated with LIPUS (1.5 MHz, 30 mW/\'CM POT.2\') at the edge, Bed Group (mean age 72.6 years, 100% women, 87.5% white) with eight ulcers (15.61 \'+ OR -\' 13.36 \'CM POT.2\') treated with the same LIPUS at the bed and Sulfadiazine Group (mean age 75.7 years, 20% women, 40% white) with seven ulcers (24,06 \'+ OR -\' 30,10 \'CM POT.2\') treated daily with 1% silver sulfadiazine cream. During treatment, they were photographed every fifteen days and analyzed with the software ImageJ to obtain the ulcerated area, Wound Healing Index (WHI) and Sphacel and Granulation Ratio (SGR) from all ulcers. At the Bed Group, it was performed a histopathological analysis (fibroblasts, inflammatory cells, blood vessels, and collagen counting) from biopsies collected at the 1st and the 45th treatment day. Data were analyzed by the (mixed effects and Poisson tests) for comparison among groups, with the level of significance set at 95% (p < 0.05). Thus, considering the slight differences found in ICU and REG of irradiated groups and GL histopathological changes, it is evident better effects in chronic venous ulcers with LIPUS treatment than conventional therapy with silver sulfadiazine 1%, independently of application site.

Region 5 is one of the most impoverished and insecure regions of Ethiopia. For decades, the region has suffered from a multitude of armed conflicts involving state and non-state actors. Region 5 is also one of the most underserved states of Ethiopia with some of the lowest levels of human development indicators nationwide. Although the adversities of poverty and conflict are widely acknowledged in their own respect, there has been little or no inquest into why poverty and conflict have prevailed under the same space for decades. Poverty and conflict have often been seen as separate phenomena that are dealt with using different sets of theories and practices in the real world. Nonetheless, a closer look at poverty and conflict in Region 5 reveals that both are strongly connected to each other. The poverty-conflict trap has been an on-going cycle in the region for the last five decades. The main intent of this research paper is analyzing the two-way relationship between poverty and conflict in Region 5. By studying this relationship, this analysis seeks to contribute to a new framework that brings peacebuilding and development closer.

本論文主要希望能夠藉由軍事準則一非作戰軍事行動(Military Operations Other Than War, MOOTW)的實務操作面向去分析「美日防衛合作新指針」中「雙方平時合作事項」，運用理論與實踐去檢證美日軍事合作對國際社會的貢獻與對台海的適用性。因為，在未來亞太安全與和平上，日本將扮演更積極角色與參加國際事務，那對曾受迫害的亞洲地區民族而言，如果無法保持客觀、理性的中立立場去觀察，那將難以抹取二次大戰軍國主義歷史的陰影，只會阻礙亞太地區邁向二十一世紀全球化的發展，具體而言，本論文具有大膽理論探討之前瞻性，屬於前沿理論(軍事準則)的概念形式。 針對亞太地區而言，在冷戰期間並沒有產生如同北大西洋公約組織及華沙公約組織一般相互對峙的軍事集團，而蘇聯所扮演的角色也僅是影響亞太安全的數個強權國家之一。冷戰結束之後，國際體系架構在冷戰結束後跨入了一個新的交替時期，東、西兩極體系彼此對抗的大環境已然消失，亞太地區也因為缺乏一個有效的多邊安全機制而失去了足以依靠的平衡槓桿。因此，亞太地區便逐漸形成一種「動態平衡」的結構。美國身為二十一世紀的唯一超強，其對於亞太地區，尤其是東北亞地區的安全政策，便成為大家所關注的焦點。其中民主國家又以美國及日本之間的共同防禦條約最值得吾人注意。 美日安保條約已逐漸成為兩國之間互動的重要架構之一，雖然美日安保條約仍以軍事安全為核心，但也進一步成為雙方經濟、政治、文化和議題發展的結構性支柱。尤其蘇聯瓦解，使得世界在可預期的未來，似乎不可能出現大規模世界核子戰爭，但是區域間的族群衝突，跨國恐怖活動、毒品走私或海盜活動等，這些「低強度衝突」(Low Intensity Conflict,LIC)的發生機率又非常的高，帶給人類和平與安全有莫大威脅，因此從實務層面思考，如何解決前述國際安全問題，才是美、日安保同盟對國際和平最大的貢獻。 針對本論文研究發現，有以下兩點建議： 一、建立國軍「非作戰軍事行動」準則 例如國內發生重大災難與跨國恐怖事件，或與國外軍事合作的行動準則，由本論文的研究發現可參考美軍準則如下：(一)可參考《美軍聯合作戰教令(Joint pub3-0》一建立(或增列)國軍聯合作戰準則中。(二)可參考《非作戰軍事行動聯合教令(Joint pub3-7)》建立國軍非作戰軍事行動教令。(三)可參考美軍陸、海、空軍對非作戰軍事行動的規範，建立國軍各軍種教範，資料如后: 1.美國陸軍野戰教範(FM100-5)。 2.美國海軍作戰準則(NDP1: Naval Warfare)。 3.美國空軍非作戰軍事行動準則(ADD2-3)。 二、積極參與區域海上人道救援行動 戰爭現已不再容易獲得國內與國際輿論之支持，反之，一國參與維持國際和平或區域海上人道救援行動，不僅容易獲得國內輿論之讚許，且可提高並贏得國際聲譽。實際上，維持國際和平與海上人道救援訓練與戰爭並無二致。並可達「寓戰於訓」的目的。而且從俄羅斯在北海的明斯克號潛艦沉沒事件，國軍在發展二代潛艦兵力的同時，對區域海上人道救援行動更應該積極參與，並列入爭取美、日、韓、澳、星等多邊國家軍事交流與訓練的第一優先項目。 關鍵詞：非作戰軍事行動 美日防衛合作新指針 低強度衝突 美日關係 / The main purpose of this thesis is looking forward to analyzing the mutual cooperation in the peace period in the " The New U.S.-Japan Defense Cooperation Guidelines " by the military principle- the real operation level of Military Operations Other than War (MOOTW). We use this theory and practice to verify the contribution of the military cooperation of between America and Japan for international society and the adaptability using in Taiwan Strait. Since the Japan will be a more active role and take part in more mission for the security and peace of Asia and Pacific area in the future. To those peoples who had suffer the oppression by Japan in Asian areas, if they can not keep an objective, rational and impartial position to observe this event,it will be hard to erase the shadow of militarism causing by Japan in the World War II.The result of this prejudice will only obstruct the progress of Asian area forward to the development of Internationalize in the 21^st century.Concretely speaking, this thesis posses a foreside in the theory discussion,and belong to the conceptualize style of the leading edge theory(military principle). Specifically speaking to the Asia and Pacific areas, it do not result the same conflict of each military ally as did between NATO and the Warsaw Treaty Organization.The former Soviet Union took part in one of the powerful countries,which influence the security of the Asian and Pacific areas. After cold war, the international structure enters to a new interchange period.The entire environment confronting between western and eastern polarities had disappeared. Asia-Pacific area lost a dependable balance lever due to lacking an effective multilateral security mechanism. Therefore, Asia- Pacific area gradually becomes a "dynamic balance" structure.The U.S. is the only one super power in the world in 21st century. The Asia-Pacific area security strategy of the U.S.,especially in northeast Asia, is the focus to other countries.For other democratic countries,the U.S.-Japan defense alliance is the axis about which the Asia-Pacific security policy of the US rotates. The U.S.-Japan Defense Cooperation Guidelines has become one of important structures of interactions between both sides.Although the U.S.-Japan Defense Cooperation Guidelines is focus on military security, furthermore, it becomes a construction pillar in economy, politics, culture and relevant developing issues on both sides. Especially,after the former Soviet Union broke down, it is almost impossible to have a nuclear war took place in the future,instated, the regional ethnic conflicts, international terrorism, narcotics dealing and smuggling and piratical activities take place very often, those bring huge threat to our peace and security. Pragmatically thinking about this, how to figure out those international issues can be the biggest contribution in terms of international peace. Based on our research,we propose the following suggestions: 1. To establish the R.O.C."Peacetime Military Operation Guidelines" for providing the Ministry of National Defense a guideline on dealing with the domestic disasters,deterring terrorism, and perusing international cooperative operations.We found that the US doctrines can provide a hands on reference: (1) The US Joint Operation Doctrine (Joint pub 3-0) can be referenced to establish the ROC joint operation doctrines. (2) The US Military Operation Other than War Joint Operation Doctrine (Joint pub 3-7) can also be referenced to establish ROC Other than War joint operation doctrines. (3) The US Military Operation Other than War Joint Operation Doctrines,for examples; US Army Operation Doctrine (FM 100-5),US Navy Operation Doctrine (NDP I: Naval Warfare), and US Air Force Other Than War Operation Doctrine (AFDD 2-3), can be referenced to establish ROC Services' Other Than War Joint Operation Doctrines. 2. To participate Asia-Pacific area rescue operations The international and nation's general public do not support the conduction of war. In contrary, the general publics always give highly respects to the organization join the international rescue operations. In facts,the procedure on conducting the war is similar to join the international rescue operation. Furthermore,the operation furnishes our troops a training event.For example, the Russia joined the rescue operation on Minske event. While the new generation submarine program is underway, we should take action and participate the area rescues, and join the international association work organized by US,Japan, Korea, Australia,and Singapore. Keywords: Military Operations Other than War (MOOTW),the New U.S.-Japan Defense Cooperation Guidelines,Low Intensity Conflict( LIC ),the U.S.-Japan Relations.

A fototerapia com laser em baixa intensidade (FTLBI) é capaz de aumentar o metabolismo celular, o que poderia influenciar na diferenciação ósseo/odontogênica das células-tronco da polpa dentária humada (hDPSCs). O PDGF e o BMP-2 são fatores de crescimento envolvidos na dentinogênese e na reparação tecidual. O PDGF tem papel importante durante o desenvolvimento embrionário, na proliferação e migração celular e na angiogênese, enquanto o BMP-2 está fortemente associado à diferenciação celular em tecidos mineralizados, como o osso e a dentina. Sendo assim, o objetivo do estudo foi analisar os efeitos da FTLBI e dos fatores de crescimento (PDGF-BB ou BMP-2), isolados ou em associação, na diferenciação ósseo/odontogênica das hDPSCs. Para o estudo hDPSCs foram cultivadas em meio regular (G1) e irradiadas (G2), meio mineralizante (G3) e irradiadas (G4), meio mineralizante contendo PDGF-BB (G5) e irradiadas (G6), meio mineralizante contendo BMP-2 (G7) e irradiadas (G8). Para os grupos irradiados, a FTLBI foi realizada no modo pontual e em contato, com um laser de diodo semi-condutor, com área de feixe de 0,028cm2 e comprimento de onda 660nm (InGaAlP-vermelho), utilizando-se os seguintes parâmetros: potência de 20mW, densidade de energia de 5J/cm2, tempo de irradiação de 7 segundos por ponto e 0,14J de energia por ponto. A expressão dos genes relacionados à diferenciação ósseo/odontogênica (DSPP, DMP-1 e OCN) através do PCR quantitativo em tempo real (qRT-PCR), a atividade da fosfatase alcalina e os depósitos de cálcio foram analisados em 3, 7 e 14 dias. Os dados obtidos foram comparados pelo teste ANOVA complementado pelo teste de Tukey (p<0,05). As culturas tratadas com meio mineralizante contendo BMP-2 e irradiadas (G8) foram as que mostraram os maiores índices de diferenciação ósseo/odontogênica nos testes realizados. As expressões de DSPP, OCN e DMP-1, ao menos em 14 dias, foram significantemente maiores no G8 que nos demais grupos experimentais, exceto os grupos G3 e G7. Estes grupos apresentaram expressões de DSPP e OCN semelhantes às do G8 em 14 dias. A maior atividade de ALP foi observada no G8 em 3 dias e a menor no mesmo grupo aos 14 dias. A maior quantidade de depósitos de cálcio também foi encontrada no G8 em 14 dias. A associação de FTLBI e BMP-2 se mostrou capaz de induzir a diferenciação ósseo/odontogênica em células-tronco de polpa dentária humana de forma mais marcante que as demais terapias isoladas ou associadas estudadas. Portanto, o uso de uma terapia associando FTLBI e BMP-2 poderia ser de relevância para o restabelecimento da fisiologia pulpar quando aplicada em casos de exposição deste tecido, uma vez que poderia favorecer a diferenciação das células indiferenciadas da polpa dentária. / Laser phototherapy (LPT) is able to increase cellular metabolism, which in turn could influence the odontogenic differentiation of dental pulp stem cells (hDPSCs). PDGF and BMP-2 are growth factors involved in dentinogenesis and tissue repair. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis, whereas BMP-2 is strongly associated with cell differentiation in mineralized tissues such as bone and dentin. The aim of this study was to analyze the effects of LPT and the growth factors PDGF-BB and BMP-2 combined or not on the odontogenic differentiation of hDPSCs. These cells were grown in regular medium (G1) and irradiated (G2), mineralizing medium (G3) and irradiated (G4), mineralizing medium containing PDGF-BB (G5) and irradiated (G6), mineralizing medium containing BMP-2 (G7) and irradiated (G8). For irradiated groups, LPT was performed in punctual and contact mode with a semiconductor diode laser, with a beam spot area of 0.028 cm2 and wavelength of 660nm (InGaAlP-visible red), using the following parameters: power of 20mW, energy density of 5J/cm2 and irradiation time of 7 seconds per point (0,14 J per point). Differentiation was assessed by the following analysis: expression of genes related to odontogenic differentiation (DSPP, DMP-1 and OCN) using quantitative real time PCR (qRT-PCR); alkaline phosphatase activity and calcium deposition using alizarin red staining in 3, 7 and 14 days. Data were compared by ANOVA and Tukey´s test (p<0.05). The cultures treated with mineralizing medium containing BMP-2 and irradiated (G8) showed the highest rate of odontogenic differentiation. The expressions of DSPP, DMP-1 and OCN genes, at least in 14 days, were significantly higher in G8 compared to all other groups, except for the groups G3 and G7. These groups showed similar expressions of DSPP and OCN than G8 in 14 days. G8 showed the highest ALP activity in 3 days and the lowest in 14 days compared to all other groups. The largest amount of calcium deposits was observed in G8 in 14 days. The most striking feature on induction of odontogenic differentiation of hDPSCs was observed when LPT was applied in association with BMP-2. Therefore, the use of a combined LPT and BMP-2 therapy could be of relevance for the re-establishment of pulp physiology when applied in cases of dental pulp exposure by promoting the differentiation of hDPSCs.

A fototerapia com laser em baixa intensidade (FTLBI) é capaz de aumentar o metabolismo celular, o que poderia influenciar na diferenciação ósseo/odontogênica das células-tronco da polpa dentária humada (hDPSCs). O PDGF e o BMP-2 são fatores de crescimento envolvidos na dentinogênese e na reparação tecidual. O PDGF tem papel importante durante o desenvolvimento embrionário, na proliferação e migração celular e na angiogênese, enquanto o BMP-2 está fortemente associado à diferenciação celular em tecidos mineralizados, como o osso e a dentina. Sendo assim, o objetivo do estudo foi analisar os efeitos da FTLBI e dos fatores de crescimento (PDGF-BB ou BMP-2), isolados ou em associação, na diferenciação ósseo/odontogênica das hDPSCs. Para o estudo hDPSCs foram cultivadas em meio regular (G1) e irradiadas (G2), meio mineralizante (G3) e irradiadas (G4), meio mineralizante contendo PDGF-BB (G5) e irradiadas (G6), meio mineralizante contendo BMP-2 (G7) e irradiadas (G8). Para os grupos irradiados, a FTLBI foi realizada no modo pontual e em contato, com um laser de diodo semi-condutor, com área de feixe de 0,028cm2 e comprimento de onda 660nm (InGaAlP-vermelho), utilizando-se os seguintes parâmetros: potência de 20mW, densidade de energia de 5J/cm2, tempo de irradiação de 7 segundos por ponto e 0,14J de energia por ponto. A expressão dos genes relacionados à diferenciação ósseo/odontogênica (DSPP, DMP-1 e OCN) através do PCR quantitativo em tempo real (qRT-PCR), a atividade da fosfatase alcalina e os depósitos de cálcio foram analisados em 3, 7 e 14 dias. Os dados obtidos foram comparados pelo teste ANOVA complementado pelo teste de Tukey (p<0,05). As culturas tratadas com meio mineralizante contendo BMP-2 e irradiadas (G8) foram as que mostraram os maiores índices de diferenciação ósseo/odontogênica nos testes realizados. As expressões de DSPP, OCN e DMP-1, ao menos em 14 dias, foram significantemente maiores no G8 que nos demais grupos experimentais, exceto os grupos G3 e G7. Estes grupos apresentaram expressões de DSPP e OCN semelhantes às do G8 em 14 dias. A maior atividade de ALP foi observada no G8 em 3 dias e a menor no mesmo grupo aos 14 dias. A maior quantidade de depósitos de cálcio também foi encontrada no G8 em 14 dias. A associação de FTLBI e BMP-2 se mostrou capaz de induzir a diferenciação ósseo/odontogênica em células-tronco de polpa dentária humana de forma mais marcante que as demais terapias isoladas ou associadas estudadas. Portanto, o uso de uma terapia associando FTLBI e BMP-2 poderia ser de relevância para o restabelecimento da fisiologia pulpar quando aplicada em casos de exposição deste tecido, uma vez que poderia favorecer a diferenciação das células indiferenciadas da polpa dentária. / Laser phototherapy (LPT) is able to increase cellular metabolism, which in turn could influence the odontogenic differentiation of dental pulp stem cells (hDPSCs). PDGF and BMP-2 are growth factors involved in dentinogenesis and tissue repair. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis, whereas BMP-2 is strongly associated with cell differentiation in mineralized tissues such as bone and dentin. The aim of this study was to analyze the effects of LPT and the growth factors PDGF-BB and BMP-2 combined or not on the odontogenic differentiation of hDPSCs. These cells were grown in regular medium (G1) and irradiated (G2), mineralizing medium (G3) and irradiated (G4), mineralizing medium containing PDGF-BB (G5) and irradiated (G6), mineralizing medium containing BMP-2 (G7) and irradiated (G8). For irradiated groups, LPT was performed in punctual and contact mode with a semiconductor diode laser, with a beam spot area of 0.028 cm2 and wavelength of 660nm (InGaAlP-visible red), using the following parameters: power of 20mW, energy density of 5J/cm2 and irradiation time of 7 seconds per point (0,14 J per point). Differentiation was assessed by the following analysis: expression of genes related to odontogenic differentiation (DSPP, DMP-1 and OCN) using quantitative real time PCR (qRT-PCR); alkaline phosphatase activity and calcium deposition using alizarin red staining in 3, 7 and 14 days. Data were compared by ANOVA and Tukey´s test (p<0.05). The cultures treated with mineralizing medium containing BMP-2 and irradiated (G8) showed the highest rate of odontogenic differentiation. The expressions of DSPP, DMP-1 and OCN genes, at least in 14 days, were significantly higher in G8 compared to all other groups, except for the groups G3 and G7. These groups showed similar expressions of DSPP and OCN than G8 in 14 days. G8 showed the highest ALP activity in 3 days and the lowest in 14 days compared to all other groups. The largest amount of calcium deposits was observed in G8 in 14 days. The most striking feature on induction of odontogenic differentiation of hDPSCs was observed when LPT was applied in association with BMP-2. Therefore, the use of a combined LPT and BMP-2 therapy could be of relevance for the re-establishment of pulp physiology when applied in cases of dental pulp exposure by promoting the differentiation of hDPSCs.

MSc (Botany) / Department of Botany / The use of disturbance in the management of Savanna is a common rangeland practice in South Africa. A two-season trial was established in June 2016 and December 2016 to evaluate the effects of disturbance of different intensities on wetland belts in the Nylsvley Nature Reserve. The disturbances were in three different belts of different grass types at different intensities. The experimental treatments consisted of two different intensities, namely; high (H) and low (L). The Cyperus amabilis belt was regarded as site 1, the Sporobolus africanus as site 2, and the Themeda triandra as site 3. Seventeen kites were contracted in each belt, two times 1 m × 1 m at high and low intensity, followed by two times 2 m × 2 m one at high intensity. The results showed that disturbance affects the topsoil by removing the soil which is rich in nutrients producing pioneer species for the first few months and then original species were seen inhibiting again. / NRF