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Prospecção química e biológica do endófito Humicola fuscoatra associado a alga vermelha Asparagopsis taxiformis para obtenção de metabólitos secundários bioativos /Mendonça, Iatã do Carmo. January 2018 (has links)
Orientadora: Dulce Helena Siqueira Silva / Banca: Alberto José Cavalheiro / Banca: Taicia Pacheco Fill / Resumo: Considerando a variedade de compostos encontrados em produtos de origem natural, o estudo da biodiversidade de um país é de interesse tanto científico quanto econômico. Sendo possível destacar os ecossistemas marinhos que apresentam biodiversidade comparável às florestas tropicais. Devido ao ambiente diferenciado, muitas vezes inóspito, os habitantes do ambiente marinho exibem características bioquímicas diferenciadas, mostrando grande potencial para bioprospecção. A importância do estudo de organismos marinhos na busca por metabólitos secundários bioativos levou à proposta de aprofundar a prospecção química do fungo endofítico Humicola fuscoatra, isolado da alga vermelha Asparagopsis taxiformis. A partir de seu extrato foi possível purificar por técnicas cromatográficas, e identificar 7 substâncias com base nos dados de ressonância magnética nuclear uni e bidimensional (RMN de 1H, RMN de 13C, TOCSY-1D, COSY, HSQC, HMBC) e espectrometria de massas (EM). Dentre estes foram identificados um composto da classe das dicetopiperazinas (P01), duas isocumarinas (P02 e P05), além de 4 substâncias não relatadas na literatura, incluindo três valerolactamas (P03, P06 e P07) e uma cicloexadienona (P04). Além dos compostos purificados por CLAE, foi possível identificar duas substâncias por cromatografia a gás acoplada a espectrometria de massas (CG-EM) sendo elas uma dicetopiperazina e um ftalato. A variedade estrutural dos compostos isolados e a grande quimiodiversidade do extrato de Humi... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Seeing the variety of compounds obtained from natural products, study a country biodiversity have a scientific interest as well as economic. It is possible to highlight marine ecosystems that present biodiversity comparable to rainforests. Due to the unique environment, often inhospitable, the organisms of the marine environments display uncommon biochemical characteristics, showing great potential for bioprospecting. The importance of the study of marine organisms in the search for bioactive compounds led to the proposal to deepen the prospection of the endophytic fungus Humicola fuscoatra, isolated from the red alga Asparagopsis taxiformis. Its extract led to the purification by chromatographic methods, and identification of 7 compounds based on their NMR spectral data obtained by uni and bidimensional experiments (1H NMR, 13C NMR, TOCSY-1D, COSY, HSQC, HMBC) and mass spectrometry (MS). One diketopiperazine (P01), two isocoumarins (P02 and P05) in addition to four novel compounds, including three valerolactams (P03, P06 and P07) and one cyclehexadienone (P04) were isolated. Moreover another diketopiperazine and one phthalate derivative were identified by gas chromatography coupled to mass spectrometry (GC-MS). The structural variety of the identified compounds associated to the rich chemodiversity observed for the Humicola fuscoatra extract reinforces the need to develop additional chemical studies of endophytic fungal strains of marine origin, since they have been providin... (Complete abstract click electronic access below) / Mestre
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Biocatalisadores de origem marinha (algas, bactérias e fungos) para redução estereosseletiva de cetonas / Biocatalysts from marine origin (algae, bacteria and fungi) for stereoselectivy reduction of ketonesAna Maria Mouad 10 September 2009 (has links)
Neste trabalho foram realizadas reações de redução de cetonas empregando diferentes organismos marinhos como biocatalisadores (algas, fungos e bactérias). Nas triagens foram utilizados derivados de acetofenonas (o-iodoacetofenona, m-iodoacetofenona, p-iodoacetofenona, o-fluoracetofenona, o-cloroacetofenona, o-bromoacetofenona, o-nitroacetofenona) e duas cetonas 1,3-dicarboniladas: a 4,4,4-triflúor-1-(furan-2-il)butano-1,3-diona e a 4,4,4-triflúor-1-(naftalen-2-il)butano-1,3-diona. As reações com as algas marinhas Bostrychia tenella e a Bostrychia radicans levaram aos álcoois com excelentes seletividades (ee >98%), contudo, obtiveram-se baixas conversões. Foram isoladas as bactérias Bt-01 (B. tenella) e Bt-02 (B. radicans), as quais catalisaram as reduções das acetofenonas com resultados similares aos obtidos com as algas. Os fungos (Br-09, Br-23, Br-27, Br-61) isolados da alga B. radicans reduziram as acetofenonas com boas seletividades e conversões. Ainda, reações de redução das acetofenonas com quatro linhagens de fungos isolados da alga Sargassum sp (SMA2-C, SMA2-8, SMA2-58, SGPY-41) levaram a obtenção dos respectivos álcoois com diferentes conversões e seletividades. As reduções das cetonas 1,3-dicarboniladas foram realizadas com as algas B. tenella e B. radicans, e com sete linhagens de fungos marinhos (Aspergillus sydowii Ce15, Aspergillus sydowii Ce19, Aspergillus sydowii Gc12, Bionectria sp Ce5, Penicillium raistrickii Ce16, Penicillium miczynskii Gc5 e Trichoderma sp Gc1). As algas e os fungos marinhos catalisaram a redução regiosseletiva e estereosseletiva das cetonas 1,3-dicarboniladas, onde ocorreu a redução do grupo α-trifluorcarbonílico. Concluiu-se que as algas e seus microrganismos associados, e os fungos marinhos têm potencial para serem utilizados como biocatalisadores em reações de redução. Este trabalho foi o primeiro estudo realizado no país envolvendo algas marinhas e seus microrganismos associados em reações de redução de cetonas, cujos resultados são bastante promissores. / In this work, were investigated the ketone reduction reactions using several marine organisms as biocatalysts (algae, fungi and bacteria). In the screening were utilized acetophenone derivatives (o-iodoacetophenone, m-iodoacetophenone, p-iodoacetophenone, o-fluoroacetophenone, o-chloroacetophenone, o-bromoacetophenone, o-nitroacetophenone) and two 1,3-dicarbonylated compounds: 4,4,4-trifluoro-1-(furan-2-yl)butane-1,3-dione and 4,4,4-trifluoro-1-(naftalen-2-yl)butane-1,3-dione. The reactions with algae Bostrychia tenella and Bostrychia radicans afforded the alcohols with high selectivities (ee > 98%), however, with low conversions. The bacteria Bt-01 and Bt-02 were isolated from algae B. tenella and B. radicans, respectively, which catalyzed the reductions of acetophenones as the same as obtained with the algae. The acetophenones were reduced by several fungi (Br-09, Br-23, Br-27, Br-61) in good selectivities and conversions. These fungi were isolated from Bostrychia radicans. In addition, the acetophenone reduction reactions were screened with four strains of fungi, which were isolated from algae Sargassum sp (SMA2-C, SMA2-8, SMA2-58, SGPY-41). The alcohols were obtained with different conversions and selectivities. The reductions of 1,3-dicarbonylated compounds were carried out with the algae B. tenella and B. radicans, and marine fungi (Aspergillus sydowii Ce15, Aspergillus sydowii Ce19, Aspergillus sydowii Gc12, Bionectria sp Ce5, Penicillium raistrickii Ce16, Penicillium miczynskii Gc5 and Trichoderma sp Gc1). The algae and marine fungi catalyzed regio- and estereoselectively reductions of the 1,3-dicarbonylated compounds. The α-trifluoromethylcarbonyl group was reduced preferentially. In conclusion, the algae and associated micro-organisms and marine fungi have potential for catalyzing ketone reduction reactions. This investigation was the first study carried out in the Brazil by using algae and associated micro-organisms in the ketone reduction reactions. The obtained results here are promising and interesting.
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Molécules colorantes naturelles issues de la biodiversité marine fongique de La Réunion : optimisation de la production, extraction et caractérisation des pigments polycétides de Talaromyces albobiverticillius 30548 / Natural coloring molecules from marine fungal biodiversity of reunion island : optimization of production, extraction and characterization of polyketide pigments from Talaromyces albobiverticillius 30548Venkatachalam, Mekala 17 November 2017 (has links)
La grande majorité des colorants alimentaires naturels, utilisés dans la formulation des aliments et des boissons, proviennent des pigments extraits de matières premières végétales. Plusieurs couleurs dérivées de plantes peuvent entraîner des problèmes de formulation. Des facteurs, comme par exemple, la région, le climat, l'environnement, la variété cultivée, ont un effet de nuances de couleurs, de résistance et surtout de stabilité dans le produit final. Par ailleurs, les champignons filamenteux du genre Monascus, Penicillium et Talaromyces sont connus comme d'excellents producteurs de pigments rouges. Ces pigments intéressent de ce fait les industries car ils sont stables, non-toxiques et peuvent être utilisés comme colorants alimentaires.La recherche présentée dans le cadre de cette thèse de doctorat concerne la description des propriétés du pigment rouge que produit la souche de Talaromyces albobiverticillius isolée du milieu marin tropical autour de l'île de La Réunion. Les plans d’expérience (DOE) et la méthodologie des surfaces de réponses (RSM) ont été utilisés pour optimiser les conditions de culture et la formulation du milieu de fermentation, dans le but d'accroître les teneurs en polykétides colorés. Douze structures différentes ont été identifiées dans des extraits intracellulaires et extracellulaires des cultures fongiques, à l'aide de séparations et d'analyses spectroscopiques (HPLC-PDA-ESI/MS et RMN). Les pigments N-thréonine-monascorubramine, N-glutaryl-rubropunctamine et PP-O figurent ainsi parmi les 12 composants.Avec la demande croissante de composés colorés naturels dans le secteur industriel, les champignons isolés du milieu marin semblent présenter de nombreux intérêts. Des essais ont ainsi été menés afin d'étudier 1) l'amélioration des conditions de fermentation en fioles agitées ou en fermenteur de 2 litres; 2) les effets de la teneur en sel marin sur la synthèse des pigments; 3) des méthodes d'extraction respectueuses de l'environnement. Globalement, ces résultats font ressortir le grand potentiel des champignons marins produisant ce colorant rouge et la possibilité d'obtenir les colorants alimentaires adaptés. / It is well known that the vast majority of food colorants used in food and beverage applications comes from the pigments synthesized by plant materials. Besides, stability of many plant-derived colors can create formulation problems. Factors such as the region, the climate, the environment, the cultivar all impact colors shade, strength and overall stability in the final product. As an alternate, fungi of the genus Monascus, Penicillium and Talaromyces are known as excellent producers of red pigments. These red pigments are of industrial interest as they are stable and non-toxic and can be used as food colorants.This present research deals with the selection of high throughput red pigment producing Talaromyces albobiverticillius as a source of polyketide based natural food colorants. Design of Experiments (DoE) and Response Surface Methodology (RSM) have been used to optimize culture conditions and media formulation of fermentation process. Using Box Behnken Design (BBD), the influence of different physical factors on pigment and biomass production was studied using potato dextrose broth as culture media. The best optimal conditions were found to be with initial pH of 6.4, temperature of 24 °C, agitation speed of 164 rpm and fermentation time of 149 h gave 47.93 ± 0.58 mg /L of orange pigment, 196.28 ± 0.76 mg / L of red pigment and 12.58 ± 0.41 g /L of dry biomass. With the application of Plackett- Burman Design (PBD), 16 different media formulations were optimized using various carbon and nitrogen sources. When Sucrose and Yeast extract was used as a basal medium at 24° C, high pigment yield was observed: 695.93 ± 0.29 mg /L of orange pigment, 738.28 ± 0.51 mg / L of red pigment and 6.80 ± 0.37 g /L of dry biomass.Twelve different compounds were detected from the HPLC-PDA-ESI/MS analysis of intracellular and extracellular pigmented extracts. In particular, N-threonine-monascorubramine, N-glutaryl-rubropunctamine and PP-O were tentatively identified among these twelve compounds; further, this work reports for the first time on the PDA, MS and NMR characterization of the here named as N-GABA-monascorubramine derivative (6-[(Z)-2-Carboxyvinyl]-N-GABA-monascorubramine) pigment bearing a cis configuration at the C10-C11 double bond, in Talaromyces albobiverticillius 30548. Attempts were made to study the effects of sea salts on pigment synthesis; sustainable green extraction methods for pigments; upscaling of fermentation from shake flasks to laboratory fermenter. All these experiments with their results were discussed briefly as individual chapters. Overall, these findings bring out the potential of marine-derived red pigment producing fungi and its possibility of obtaining tailor made food colorants.
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Marine biotechnology : evaluation and development of methods for the discovery of natural products from fungiPather, Simisha 18 June 2013 (has links)
One of the major impediments in the development of marine natural products is the provision of biologically active natural products in sufficient quantity for complete pharmacological evaluation, clinical trials and eventual commercial production. Marine microorganisms show great promise in providing a renewable source of biologically active natural products. The main aim of this study was to develop and evaluate methods for the isolation, identification and cultivation of marine fungi from the South African marine environment for the production of biologically active secondary metabolites. Twenty-four species of fungi were isolated from marine algae collected from the intertidal zone near Port Alfred, South Africa. The fungi were cultivated in small-scale under static and agitated conditions and their crude intra- and extracellular organic extracts were screened by ¹H NMR and a series of bioassays. Using this as a basis, one isolate was selected for further study. By analyses of the lTS1 region of the ribosomal DNA, the fungal isolate was identified as a marine-derived isolate of Eurotium rubrum (Aspergillus ruber). Although E. rubrum has been isolated from the marine environment, no investigations have been undertaken to determine the adaptation of these isolates to the marine environment. In order to optimise productivity, creativity and incubation time, the fungus was cultivated in small-scale using a variety of carbon (glucose, fructose, lactose, sucrose, marmitol and maltose) and nitrogen sources (ammonium tartrate, urea, peptone and yeast extract). An HPLC-DAD method was developed to assess the metabolic creativity and productivity under different fermentation conditions. Distinctive variations in the range and yield of metabolites produced as well as morphology and growth time were observed. The crude extracts from all fermentations were combined and six known compounds were isolated by reversed-phase chromatography and their structures elucidated by spectroscopic techniques. The known compounds were fIavoglaucin, aspergin, isodihydroauroglaucin, isotetrahydroauroglaucin, neoechinuline A and physcion. Neoechinuline A, isodihydroauroglaucin and isotetrahydroauroglaucin showed activity against oesophageal and cervical cancer cell lines.
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Valorisation de biocides d’invertébrés marins méditerranéens / Secondary metabolites from Mediterranean marine invertebratesSfecci, Estelle 04 June 2018 (has links)
Ces travaux de thèse portent sur l’étude chimique d’espèces marines échantillonnées en Méditerranée. Dans le cadre du projet VALBIM, co-financé par la région PACA et en étroite collaboration avec la société BioPreserv à Grasse mais également avec la Mycoteca Universitatis Taurinensis (MUT) de l’Université de Turin, nous avons isolé et caractérisé 21 molécules dont 14 se sont avérées nouvelles. L’étude de l’algue Caulerpa taxifolia a conduit à la caulerpényne et à quatre nouveaux dérivés de l’acide pyruvique sulfatés. Les travaux sur l’ascidie Polysyncraton sp. ont conduit aux bolascidines A-D, quatre nouveaux métabolites de type bolaamphiphiles dont il n’existe pas à notre connaissance d’équivalent naturel ou synthétique. La première étude chimique de l’éponge Hexadella racovitzai a conduit à deux dérivés de la bromotyrosine, la psammapline A et une nouvelle molécule, la 4-Osulfatocyclobispsammapline A. Par ailleurs, trois nouvelles crambescines C1, conjointement avec une crambescine A2 déjà répertoriée, ont été isolées de Crambe crambe. L’étude du champignon marin Stachybotrys chartarum, isolé de l’éponge Aplysina cavernicola, a conduit à la satratoxine H et à deux nouveau dérivé, la 2,3-dihydrosatratoxine H et l’épi-chartarutine G, ainsi qu’à trois dérivés stachybotrylactame déjà répertoriés dans la bibliographie. Les différents métabolites ont été évalués pour leurs propriétés antibactériennes et cytotoxiques. Ces travaux ont bénéficié de nouvelles approches analytiques (calculs des spectres de dichroïsme circulaire électroniques par TDDFT, réseaux moléculaires) permettant de renforcer l’intérêt des études liées à l’écologie chimique et à la recherche de nouvelles molécules bioactives potentiellement valorisables dans différents domaines. / This PhD work focuses on the chemical study of marine species sampled in the Mediterranean Sea. As part of the VALBIM project, cofinanced by the region PACA and in close collaboration with the SME BioPreserv in Grasse but also with the Mycoteca Universitatis Taurinensis (MUT) of the University of Turin, we have isolated and characterized 21 molecules in total.14 of them were never reported before. The study of the seaweed Caulerpa taxifolia led to caulerpenyne and four new sulphated pyruvic acid derivatives. The work on the ascidian Polysyncraton sp. led to bolascidins A-D, four new metabolites which belong to the bolaamphiphile family, which, to the best of our knowledge, have no natural or synthetic equivalent. The first chemical study of the sponge Hexadella racovitzai led to two bromotyrosine derivatives, psammapline A and a new molecule, 4-O-sulfatocyclobispsammapline A. In addition, three new C1 crambescins, together with one crambescine A2 already reported, have been isolated from Crambe crambe. For all isolated marine invertebrate metabolites, the producer(s) remain(s) to be explored. The study of the marine fungus Stachybotrys chartarum, isolated from the sponge Aplysina cavernicola, led to satratoxin H and three stachybotrylactam derivatives already reported in the literature, and to 2,3-dihydrosatratoxin H and epi-chartarutine G, two new natural products. The different metabolites were evaluated for their antibacterial and cytotoxic properties. This work benefited from new analytical approaches (calculations of electronic circular dichroïsm spectra by TDDFT, molecular networking) and reinforced the interest of studies related to chemical ecology and search for new bioactive molecules to be valorized in different areas.
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Allene, Dihydronaphthalenon-Derivate und andere Sekundärmetabolite aus Pilzen mariner Habitate sowie Beiträge zu deren Biosynthese / allenes, dihydronaphthalenone-derivatives and further secondary metabolites from marine fungi as well as contributions to their biosynthesisWolff, Diana 03 November 2004 (has links)
No description available.
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