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Erythroxylum pungens O. E. shulz: prote?mica total e bioprospec??o de alcaloides trop?nicos / Erythroxylum pungens O. E. shulz: total proteomics and bioprospection of tropane alkaloidsPereira, Gabrielle Macedo 25 August 2017 (has links)
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Previous issue date: 2017-08-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O bioma Caatinga ? exclusivamente brasileiro, marcado pelo clima semi?rido. Este bioma se destaca pelo alto ?ndice de endemismo e desconhecimento cient?fico ao seu respeito. Para sobreviver neste ambiente, as plantas desenvolveram mecanismos intr?nsecos de percep??o dos sinais ambientais externos e suas respectivas respostas metab?licas. Dentre as esp?cies de ocorr?ncia no bioma Caatinga, como fonte de alcaloides bioativos, destaca-se Erythroxylum pungens, que apresenta potencial pouco explorado. Assim, o objetivo deste trabalho ? investigar o proteoma total e o perfil metab?lico de E. pungens, aliado ? bioprospec??o de alcaloides trop?nicos com potencial citot?xico. Para isso, no cap?tulo 1 identificou-se, por espectrometria de massas, sete alcaloides trop?nicos conhecidos; 3-(2-metilbutiriloxi)tropan-6, 7-diol como tamb?m 3-(2-metilbutiriloxi)nortropan-6, 7-diol foram isolados e caracterizado por RMN 1D e 2D pela primeira vez. N,N-Dimetil-1-H-indol-3-etanamina foi isolado e caracterizado a partir de ra?zes de E. pungens. Al?m disso, verificou-se que alcaloides isolados de E. pungens, frente a cinco linhagens celulares, apontaram potencial seletivo de 3-(2-metilbutiriloxi)tropan-6, 7-diol contra c?lulas de tumorais de pr?stata. Para melhor conhecer a esp?cie e, com vistas na produ??o de alcaloides trop?nicos, trabalhou-se o proteoma e perfil alcalo?dico no cap?tulo 2. Dessa forma foi poss?vel identificar 1746 prote?nas nas folhas, 1779 no caule e 1026 na raiz de E. pungens. Atrav?s da recupera??o dos termos no Gene Ontology, observou-se os processos nos quais estas prote?nas est?o envolvidas, verificando o mesmo perfil de processos para os tr?s ?rg?os de E. pungens, com destaque para respostas a estresses bi?ticos, abi?ticos, qu?micos, al?m das respostas ao estresse oxidativo. Foram observados n?veis significativos de prote?nas envolvidas na fotoss?ntese das folhas de E. pungens, fotorrespira??o, al?m prote?nas relacionadas ?s respostas ao estresse: prote?nas da fam?lia 14-3-3, peroxidases, catalases, super?xido-dismutase e prote?nas de choque t?rmico, que tamb?m podem atuar no turnover prote?co. Ainda foram identificadas cinco prote?nas hom?logas a tropinona redutase na folha, e produ??o de alcaloide em todos os ?rg?os. No cap?tulo 3, por meio do experimento com esp?cimes de E. pungens submetidas a estresse h?drico por suspens?o de rega em casa de vegeta??o, notou-se que esta esp?cie investe no aumento de prote?nas relacionadas ?s respostas ao estresse h?drico, aumenta significativamente o teor de prolina livre no citosol. Ademais, mat?m a produ??o de alcaloides que parecem estar mais relacionado a fase de desenvolvimento da planta. Com isso, os resultados deste trabalho busca contribuir para a fitoqu?mica e fisiologia de E. pungens, assim como promove o uso racional da esp?cie, e consequentemente a conserva??o do bioma Caatinga. / Caatinga biome is exclusive brazilian, marked by semi-arid climate. This biome stands out for the high endemism index and scientific unfamiliarity.To survive in this environment with peculiar edaphoclimatic conditions, plants show evolution of intrinsic mechanisms of external environmental signals perception and their respectives metabolic responses. Among the occurrence genera in Caatinga biome, as bioactive alkaloids source, the Erythroxylum pungens species, which has unexplored potential, is outstanding. Thus, the objective of this work is to investigate total proteome and metabolic fingerprint of E. pungens, together with tropane alkaloids bioprospection with cytotoxic potential. For this, in chapter 1 it was possible to identify, by mass spectrometry, seven known tropane alkaloids; 3-(2-methylbutyryloxy)tropan-6, 7-diol as well as 3-(2-methylbutyryloxy)tropan-6, 7-diol were isolated and characterized by 1D and 2D NMR for the first time. N,N-Dimethyl-1-H-indol-3-ethanamine was isolated and characterized from roots of E. punctures. In addition, it was found that alkaloids isolated from E. pungens, against five cell lines, indicated selective potential of 3-(2-methylbutyryloxy)tropan-6, 7-diol against prostate tumor cells. In chapter 2, it was possible to identify 1746 proteins in the leaves, 1779 in the stem and 1026 in the root of E. pungens. Through the recovery of the GO terms, it was possible to evaluate processes in which these proteins are involved, verifying the same process profile for three organs of E. pungens, with emphasis on biotic, abiotic, chemical stress responses, as well as responses to oxidative stress. Were observed significant proteins levels involved in photosynthesis of E. pungens leaves, photorespiration, as well as many proteins related to stress responses: proteins from 14-3-3 family, peroxidases, catalases, superoxide dismutase and shock proteins, which can also act on protein turnover. Were still identificated five proteins homologous to tropinone reductase, and alkaloid production in all organs suggesting that even under chronic stress conditions, E. pungens maintains the production of these metabolites. In chapter 3, through specimens submission experiment of E. pungens to water stress by irrigation suspension in greenhouse, it was observed that this species invest in the increase of proteins related to the responses to water stress, it significantly increases free proline content in cytosol which functions as an important osmoprotector. Furthermore, maintains alkaloids production that appear to be more related to development stage of the plant than stress condition in which it is inserted. The results of this work seek to contribute to knowledge of E. pungens phytochemistry and physiology, as well as to promote the species and consequently conservation of Caatinga biome.
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Morfog?nese in vitro e triagem fitoqu?mica de Myracrodruon urundeuva Fr. All.Silva, Tecla dos Santos 28 September 2017 (has links)
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Previous issue date: 2017-09-28 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / Myracrodruon urundeuva Fr. All. (Anacardiaceae) is an endangered tree that has wood and medicinal potential. This work aimed to study the induction of in vitro buds of M. urundeuva and to analyze the calogenesis process, as well as qualitatively evaluate the chemical composition of different organs of the species submitted to different culture conditions. In the induction of shoots, silver nitrate (AgNO3) and plant regulators 6-benzylaminopurine (BAP), kinetin (KIN), meta-topoline (mT) and naphthaleneacetic acid (NAA) were evaluated using different explants (cotyledons, petiole, nodal, cotyledonary and apical segments). For calogenesis, combinations of 2,4-D, KIN and glutamine in leaf explants were tested. The callus growth curve was performed for 56 days (7 day intervals) and the reducing sugars (RS), sucrose and total soluble sugars (TSS) were quantified. For the phytochemical study, we used methanolic extracts from material grown in vitro, in a greenhouse and in a natural environment. It is possible the induction of buds in M. urundeuva from the explant cotyledon node with the use of BAP combined with NAA. Calogenesis is potentiated with the combination of 2,4-D, KIN and glutamine in the nutrient medium. The growth curve of the callus is sigmoidal. The carbohydrate content, except for RA, presents a distinct behavior, varying according to the growth curve phases of the callus. The type of cultivation influences the yield of extracts of M. urundeuva and there are differences in the production of secondary metabolites between the different organs of the species. / Myracrodruon urundeuva Fr. All. (Anacardiaceae) ? uma ?rvore amea?ada de extin??o que possui potencial madeireiro e medicinal. Este trabalho objetivou estudar a indu??o de brotos in vitro de M. urundeuva e analisar o processo de calog?nese, bem como avaliar qualitativamente a composi??o qu?mica de diferentes ?rg?os da esp?cie submetida a distintas condi??es de cultivo. Na indu??o de brotos avaliou-se o uso do nitrato de prata (AgNO3) e dos reguladores vegetais 6-benzilaminopurina (BAP), cinetina (CIN), meta-topolina (mT) e ?cido naftalenoac?tico (ANA) sob distintos explantes (cotil?dones, pec?olo, segmentos nodais, cotiledonares e apicais). Para calog?nese testou-se combina??es de 2,4-D, CIN e glutamina em explantes foliares. Realizou-se a curva de crescimento dos calos durante 56 dias (intervalos de 7 dias) e quantificou-se os a??cares redutores (AR), sacarose e a??cares sol?veis totais (AST). Para o estudo fitoqu?mico, utilizou-se extratos metan?licos advindos de material cultivado in vitro, casa de vegeta??o e em ambiente natural. ? poss?vel a indu??o de brotos em M. urundeuva a partir do explante n? cotiledonar com o uso de BAP combinado com ANA. A calog?nese ? potencializada com a combina??o de 2,4-D, CIN e glutamina no meio nutritivo. A curva de crescimento dos calos apresenta forma sigmoidal. O conte?do de carboidratos, excetuando os AR, apresenta comportamento distinto, variando de acordo com as fases da curva de crescimento dos calos. O tipo de cultivo influencia no rendimento de extratos de M. urundeuva e existem diferen?as na produ??o de metab?litos secund?rios entre os diferentes ?rg?os da esp?cie.
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Bioprospec??o qu?mica da esponja callyspongia vaginalis (Callyspongiidae)Ara?jo, Rusceli Diego de 05 April 2013 (has links)
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Previous issue date: 2013-04-05 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Recently, marine organisms have attracted attention because of the
complexity and potent biological activity from your secondary metabolites. Our
planet has 80% it surface covered by oceans and seas, therefore, housing a
wide number of different forms of life, among them, the sponges. These sessile
and filtrating animals, according to numerous researches, come showing like
true chemistry factories. The substances from these animals, sometimes show
as news targets to therapeutics agents, and some countries has already use
them for treatment of some diseases. Further of the secondary metabolites, the
polysaccharides of marine origin also have been target of studies, because the
presence of the sulfates groups in its molecules. Polysaccharides with differents
biological activities have been related in a large number of researches. Actually,
many studies show the sponges as source of promising medicine. These
studies inspire new researches, because the few number of sponges species
studied until now. Because of that, the present work shows the chemistry
prospection of the sponge Callyspongia vaginalis. Chromatographic methods in
silica gel allowed the isolations of two secondary metabolites: the known β-
sitosterol and a ceramide, no reported in the genus Callyspongia, previously.
The analysis of the their lipid extracts show different kinds of fatty acids with a
variety of chain length (saponifiable fraction), and others metabolites like
Lupenone and stigmasterol, also unprecedented in the genus. The
Polysaccharide characterization and the elucidation of the secondary
metabolites acquired through of chromatography analysis (CC, molecular
exclusion) and spectrometric (NMR 1H and 13C, mass, IR), respectively and
comparison with literature data / A diversidade estrutural de subst?ncias de origem marinha, associadas
as suas atividades biol?gicas, t?m atra?do aten??o de pesquisadores do mundo
inteiro. Dentre os organismos estudados atualmente dois grupos de
invertebrados marinhos, as asc?dias e as esponjas, merecem destaque. Estes
animais s?sseis e filtradores, segundo in?meras pesquisas, v?m se mostrando
verdadeiras f?bricas qu?micas , produzindo subst?ncias que por vezes
apresentam-se como aspirantes a agentes terap?uticos, com efic?cia cl?nica
comprovada. Al?m dos metab?litos secund?rios, os polissacar?deos de origem
marinha tamb?m tem sido alvo de estudos, sendo relatados diversos
compostos sulfatados e com diferentes atividades biol?gicas associadas a
estes. Tendo em vista a pequena quantidade de esp?cies de esponjas
estudadas at? o momento, comparado ao potencial qu?mico destes
organismos, o presente trabalho apresenta a prospec??o qu?mica da esponja
Callyspongia vaginalis. T?cnicas cl?ssicas de cromatografia em gel de s?lica
permitiram o isolamento de dois metab?litos secund?rios: o β-sitosterol e uma
ceramida, ambos in?ditos no g?nero Callyspongia. A an?lise das fra??es
lip?dicas mostraram diferentes tipos de ?cidos graxos com variados tamanhos
de cadeia (fra??o saponific?vel), e de outros metab?litos como lupenona e
estigmasterol, tamb?m in?ditos no g?nero em quest?o. O polissacar?deo obtido
atrav?s do material animal tamb?m foi isolado e, como amplamente relatado na
literatura apresentou-se sulfatado, caracter?stica diferencial dos polissacar?deos
de origem marinha. A caracteriza??o do polissacar?deos e a elucida??o
estrutural dos metab?litos secund?rios foram realizadas atrav?s da an?lise dos
dados de espectrometria de massa, Infravermelho e Resson?ncia Magn?tica
Nuclear de Hidrog?nio-1 e Carbono-13, atrav?s de sequ?ncias de pulsos uni e
bidimensionais e compara??o com dados da literatura
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Fatores Antinutricionais em Tr?s Esp?cies de Leguminosas Forrageiras. / Antinutritional Factors in Three Forage Legumes Species.Nepomuceno, Delci de Deus 13 January 2009 (has links)
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Previous issue date: 2009-01-13 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work was divided into three chapters; the first one was carried out for identifying
secondary metabolites classes on three forage legumes: Pueraria phaseoloides (puero),
Macrotyloma axillare (archer) and Neonotonia wightii (perennial soybean). These sorts of
metabolites when present in plants may cause to animal adverse effects resulting ingestion and
digestion decreasement and nutrients bioavailabity named antinutritional factors as well as may
systematically induce to animal or herd performance decreasement. For this stud,
phytochemical prospection, Nuclear Magnetic Resonance Analysis (NMRA) and Infrared
Spectroscopy (IS) were performed. Following secondary metabolites classes: organic acids,
reducing sugars, depsidians and depsidones, coumarine derivatives, steroids and triterpenoids,
saponins, cardiac glycosides, saccharides and tannins on three forage legumes species were
identified. Puero e perennial soybean for alkaloids presence were compatible. According
bibliographic references, secondary metabolites classes have presented antinutritional effects
compounds, some of totally dependent on intake and specie like reducing sugars and
saccharides responsible for antinutritional effects on monogastric animals as well. The second
chapter of this survey was carried out for quantifying condensed tannin content by Stiasny
Reaction and for evaluating puero, archer and perennial soybean in vitro foam stability. For
tannin quantification separated portions of entire plant, leaves, and stem by Simple
Randomized Design with legumes allocated on subdivided parcels and portions on subparcels
was used. By analysis of covariance, obtained result average, as well as interactions effects
between legumes and portions by Tukey Test (P<0.05) were achieved. In vitro foam stability
and yielding evaluation due to produced foam by legumes considered ruminant foamy
meteorism potential etiological agent was performed. Simple Randomized Design consisting by
subdivided parcels with three replications each was carried out where portions were composed
by entire plant, leaves and stem as well as crude methanol extract related to parcels and
remaining time (0, 5, 10, 30 and 60 minutes) for monitoring volume to subparcels. By Tukey
Test (P<0.05) each portion averages within each time were compared and by analysis of
variance results related to volume and remaining time were submitted to. By statistical analysis
of Regression, saved volume effect during time and legumes interaction and checking time
were evaluated as well as foam stability interaction in relation to remaining foam was
estimated. Condensed tannin content ranged from 1 to 1.6%. In regard to perennial soybean and
archer, foam stability produced by entire plant, leaves, stem and legumes methanol extract was
considered constant, nevertheless puero showed significative result, creating the following
regression equations y= -2.0111x 44.984 for leaf; y= -2.4x 37.957 for stem; y= -2.9049 x
31.779 for plant entire and y= -15.383x 125.31 for methanol extract. The aim of third chapter
was to evaluate fungi association to legumes: Pueraria phaseoloides (puero), Neonotonia
wightii (perennial soybean), Macrotyloma axillare (archer), Calopogonium mucunoides
(calopo) and Arachis pintoi cv Amarillo (pinto), however, due to substances over legumes
leaves presenting disgusting feature they have been considered unappropriate to animal
feeding. According Barnett and hunter (1990) and Pitt and Hocking (1997) fungi amorphous
reproduction elements were used. For fungi macroscopic and microscopic identification,
Imprint tape technique, NaOH and blue cotton were used. The following fungi: Alternaria spp,
Phitomyces chartarum, Nigrospora spp, Cladosporium spp, Mucor spp, Fusarium spp,
Pseudomicrodochium spp, Tetraploa spp, Acremonium spp, Aspergillus niger, Curvularia spp
and Micelia sterilia were reported, at all. / Este trabalho foi dividido em tr?s cap?tulos, o primeiro realizou-se com o objetivo de identificar
as classes de metab?litos secund?rios presentes em tr?s leguminosas forrageiras Pueraria
phaseoloides (kudzu tropical), Macrotyloma axillare (macrotiloma) e Neonotonia wightii (soja
perene). Metab?litos estes, que quando presentes em plantas podem provocar efeitos adversos ao
animal ocasionando diminui??o na ingest?o, digest?o e biodisponibilidade de nutrientes os que
lhes conferem a denomina??o de fatores antinutricionais, estes efeitos podem repercutir de forma
sist?mica acarretando diminui??o do desempenho animal ou rebanho. Para este estudo foi
realizada a prospec??o fitoqu?mica, an?lise de Resson?ncia Magn?tica Nuclear de Hidrog?nio
(RMN 1H) e Espectroscopia no Infravermelho (IV). Foram identificadas as seguintes classes de
metab?litos secund?rios: ?cidos org?nicos, a??cares redutores, deps?dios e depsidonas, derivados
da cumarina, ester?ides e triterpen?ides, saponinas, glicos?deos card?acos, sacar?deos e taninos
nas tr?s esp?cies estudadas. O kudzu tropical e soja perene apresentaram ainda sinais compat?veis
para a presen?a de alcaloides. As classes de metab?litos secund?rios estudadas apresentam
compostos com efeitos antinutricionais para os animais, alguns destes efeitos s?o dependentes da
quantidade ingerida e da esp?cie animal como o caso dos sacar?deos e ac?cares redutores que
possuem efeitos antinutricionais em monog?stricos segundo literaturas consultadas. O segundo
cap?tulo deste trabalho teve como objetivo quantificar o teor de tanino condensado atrav?s da
Rea??o de Stiasny e avaliar a estabilidade in vitro da espuma formada pelas leguminosas,
kudzu tropical, macrotiloma e soja perene. Para a quantifica??o do tanino, foram tomados em
separados as por??es, planta inteira, folhas e haste, utilizou-se um delineamento inteiramente
casualizado com parcelas subdivididas alocando-se as leguminosas nas parcelas e as por??es nas
subparcelas. A m?dia do resultado obtido foi submetida ? an?lise de vari?ncia e comparadas pelo
Teste de Tukey (P<0,05), os efeitos das intera??es entre leguminosas e por??es foram
desdobrados pelo teste de Tukey (P<0,05) a avalia??o da produ??o e estabilidade da espuma in
vitro foi realizada devido ? espuma produzida por leguminosas ser referida como poss?vel
agente etiol?gico do meteorismo espumoso em ruminantes. Neste estudo utilizou-se o
delineamento inteiramente casualizado em parcelas subdivididas com tr?s repeti??es cada, onde
as por??es compostas por planta inteira, folhas, e haste e o extratos metan?lico bruto foram
consideradas as parcelas e o tempo de repouso (0, 5, 10, 30 e 60min) onde aferiu-se o volume as
subparcelas. O resultado referente ao volume produzido e conservado durante o tempo de
repouso foi submetido ? an?lise de vari?ncia e as m?dias de cada por??o dentro de cada tempo
foram comparadas pelo teste de Tukey (P<0.05) o efeito do volume conservado durante o tempo
e a intera??o entre leguminosas e tempo de aferi??o foi desdobrados pela an?lise de regress?o e a
intera??o da estabilidade da espuma formada foi avaliada em fun??o da porcentagem de espuma
remanescente. Como resultado deste estudo o teor de tanino condensado variou de 1 a 1,6%.
Quanto ? estabilidade da espuma produzida pelas por??es planta inteira, folha, haste e extrato
metan?lico das leguminosas foi considerada est?vel para as por??es referentes ? soja perene e
macrotiloma, entretanto o kudzu apresentou um resultado significativo para a estabilidade da
espuma para as quatros por??es em estudos, gerando as equa??es de regress?o y=-2,0111x44,984
para a por??o folha; y= -2,4x37,957 para a por??o haste; y= -2,9049x31,779 para a por??o planta
inteira e para o extrato metan?lico a equa??o y= -15,383x 125,31. O objetivo da realiza??o do
terceiro cap?tulo deste estudo foi avaliar a associa??o de fungos com as leguminosas: Pueraria
phaseoloides (kudzu tropical), Neonotonia wightii (soja perene), Macrotyloma axillare (macrotiloma), Calopogonium mucunoides (calopog?nio) e Arachis pintoi cv.amarillo
(amendoim forrageiro) devido ? presen?a de subst?ncias encontradas depositadas sobre as folhas
das leguminosas as quais conferiram aspecto repugnante para inclus?o na alimenta??o animal.
Para esta avalia??o foram utilizados os elementos de reprodu??o amorfas dos fungos de acordo
com as metodologias de Barnett e Hunter, (1990) e Pitt e Hocking, (1997). Na identifica??o
observaram-se a macroscopia e microscopia dos fungos utilizando Imprint com fitas adesivas ou
lam?nulas de vidro, NaOH e Azul de algod?o, diretamente do material obtido das leguminosas e
das col?nias desenvolvidas em Agar Sabouraud Dextrose e Agar Simples, sendo identificados os
fungos Alternaria spp, Phitomyces chartarum, Nigrospora spp, Cladosporium spp, Mucor spp,
Fusarium spp, Pseudomicrodochium spp, Tetraploa spp, Acremonium spp, Aspergillus niger,
Curvularia spp e Mic?lia ster?lia.
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Morfoanatomia, composi??o qu?mica e atividade biol?gica do ?leo essencial de esp?cies nativas de LippiaOliveira, Ariana Reis Messias Fernandes de 17 December 2014 (has links)
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Previous issue date: 2014-12-17 / The aim of this study was to characterize the morphology, production, content, chemical composition and bioactive activity of essential oils of Lippia bromleyana, Lippia lasiocalycina, Lippia insignis and Lippia thymoides, endemic species of the Bahia semi-arid. The species were grown in the Experimental Station Horto Florestal State University of Feira de Santana (UEFS) in the city of Feira de Santana - Bahia. The essential oils are extracted from dry leaves and inflorescences, by hydrodistillation in Clevenger apparatus, and chemical composition was determined by GC/MS and GC/FID. Were carried out quantitative and qualitative morphological characterizations, in addition to agronomic characterization. The leaf anatomy, types and frequency of hair were observed in binocular microscope and electronic scanning light. The antioxidant activity was assessed by the ability of the substances present in the sample capture the free radical DPPH, using five concentrations of essential oils (2, 6, 10, 14 and 18 mg mL1) and the antifungal activity by mycelium growth in vitro testing five essential oil concentrations (0.25, 0.50, 0.75, 1.0 and 1.25 ?L mL-1). In conditions where the study was conducted, it can be concluded that there are morphological differences between species in all traits, except only the number of flowers per inflorescence; there agronomic differences for all traits with L. lasiocalycina stood out in relation to the variable oil yield, while L. insignis and L. thymoides regarding the essential oil content; were identified six types of glandular trichomes one, two and tetracelular and three types of trichomes; the species L. bromleyana presents as differential anatomical absence of trichomes on the abaxial surface; L. thymoides has glandular trichomes with irregular contours on both sides, distinguishing it from other species; the frequency of trichomes on the abaxial surface is higher in species L. insignis and L. lasiocalycina, which are more anatomically similar; the major compounds found in the samples of essential oils of L. bromleyana, L. lasiocalycina, L. insignis and L. thymoides were piperitone oxide and limonene; E-ocimenona, myrcenone, myrcene, ?-myrcene and ?-cymene; thymol, myrcenone and E-ocimenona; and ?-caryophyllene, germacrene D, respectively; L. insignis and L. bromleyana stood out in relation to the antioxidant and antifungal activity, respectively. / O objetivo desse trabalho foi caracterizar a morfologia e a produ??o, teor, composi??o qu?mica e atividade bioativa de ?leos essenciais de Lippia bromleyana, Lippia lasiocalycina, Lippia insignis e Lippia thymoides, esp?cies end?micas do semi?rido baiano. As esp?cies foram cultivadas na Unidade Experimental Horto Florestal da Universidade Estadual de Feira de Santana (UEFS), na cidade de Feira de Santana ? Bahia. Os ?leos essenciais foram extra?dos de folhas e infloresc?ncias secas, por meio da hidrodestila??o em aparelho de Clevenger e a composi??o qu?mica determinada por CG/EM e CG/DIC. Foram realizadas caracteriza??es morfol?gicas quantitativas e qualitativas, al?m da caracteriza??o agron?mica. A anatomia foliar, tipos e frequ?ncia de tricomas foram observados em microsc?pio de luz binocular e eletr?nico de varredura. A atividade antioxidante foi avaliada pela capacidade das subst?ncias presentes na amostra captarem o radical livre DPPH, utilizando cinco concentra??es dos ?leos essenciais (2, 6, 10, 14 e 18 mg mL-1) e a atividade antif?ngica pelo crescimento miceliano in vitro, testando cinco concentra??es do ?leo essencial (0,25, 0,50, 0,75, 1,0 e 1,25 ?L mL-1). Nas condi??es em que foi realizado o estudo, pode-se concluir que existem diferen?as morfol?gicas entre as esp?cies em todas as caracter?sticas avaliadas, com exce??o apenas para o n?mero de flores por infloresc?ncia; existem diferen?as agron?micas para todos os caracteres avaliados, sendo que L. lasiocalycina se destacou em rela??o ? vari?vel rendimento de ?leo, enquanto que L. insignis e L. thymoides em rela??o ao teor de ?leo essencial; foram identificados seis tipos de tricomas glandulares uni, bi e tetracelular e tr?s tipos de tricomas tectores; a esp?cie L. bromleyana apresenta como diferencial anat?mico aus?ncia de tricomas tectores na face abaxial; L. thymoides possui tricomas glandulares com contornos irregulares em ambas as faces, distinguindo-a das demais esp?cies; a frequ?ncia de tricomas tectores na face abaxial ? superior nas esp?cies L. insignis e L. lasiocalycina, as quais s?o mais semelhantes anatomicamente; os compostos majorit?rios encontrados nas amostras dos ?leos essenciais de L. bromleyana, L. lasiocalycina L. insignis e L. thymoides foram: ?xido de piperitona e limoneno; E-ocimenona, mircenona, mirceno, ?-mirceno e ?-cimeno; timol, mircenona e E-ocimenona; ?-cariofileno e germacreno D, respectivamente; L. insignis e L. bromleyana se destacaram em rela??o ? atividade antioxidante e antif?ngica, respectivamente.
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