Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-27T22:26:12Z No. of bitstreams: 1 DISSERTA??O Val?ria Silva G?es.pdf: 1629398 bytes, checksum: 6e130737f6d57007b90ea3ae8de5042b (MD5) / Made available in DSpace on 2016-09-27T22:26:12Z (GMT). No. of bitstreams: 1 DISSERTA??O Val?ria Silva G?es.pdf: 1629398 bytes, checksum: 6e130737f6d57007b90ea3ae8de5042b (MD5) Previous issue date: 2015-03-31 / The Lamiaceae family has a great importance in the Brazilian semiarid region due to their species of pharmacological properties. Martianthus leucocephalus (Mart. Ex Benth.) JFB Pastore belongs to the Lamiaceae family and has had the antimicrobial activity of essential oil proven. However, this species lacks pharmacological studies that evaluate its biological potential. Thus, the aim of this study was to evaluate the effect on the central nervous system, the acute toxicity, as well as investigate the antinociceptive and anti-inflammatory of Martianthus leucocephalus extract. The possible mechanism of action of species was also studied. The experiments were performed in the Chemistry Laboratory of Bioactive Natural Products and in the Laboratory of Pharmacology at the State University of Feira de Santana. The effect on the central nervous system was evaluated through Behavioral Pharmacological Screening by Rota Rod test. The acute toxicity was determined using the protocol Organization for Economic Cooperation and Development (OECD - 420/2001). The antinociceptive activity was investigated in the tests of acetic acid, formalin and hot plate. The mechanism of action was determined using the opioid antagonist naloxone in the hot plate model. The study of anti-inflammatory activity was performed using the paw edema induced by carrageenan tests and capillary permeability induced by acetic acid. The results were analyzed by analysis of variance (ANOVA). Martianthus leucocephalus showed no acute toxicity or muscle relaxant effects action on the motor system. The antinociceptive and anti-inflammatory properties were found significantly compared to the control group animals. / A fam?lia Lamiaceae tem uma grande import?ncia na regi?o do semi?rido brasileiro devido ?s suas esp?cies detentoras de propriedades farmacol?gicas. Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore pertence ? fam?lia Lamiaceae e j? teve a atividade antimicrobiana do seu ?leo essencial comprovada. No entanto, a referida esp?cie carece outros de estudos farmacol?gicos que avaliem seu potencial biol?gico. Diante disso, o objetivo desse trabalho foi avaliar o efeito sobre o sistema nervoso central, a toxidade aguda, bem como investigar as atividades antinociceptiva e anti-inflamat?ria de extratos de Martianthus leucocephalus proveniente de cultivo. O poss?vel mecanismo de a??o da esp?cie tamb?m foi estudado. Os experimentos foram realizados no Laborat?rio de Qu?mica de Produtos Naturais e Bioativos e no Laborat?rio de Farmacologia da Universidade Estadual de Feira de Santana. O efeito sobre o sistema nervoso central foi avaliado por meio da Triagem Farmacol?gica Comportamental e pelo teste do Rota Rod. A toxidade aguda foi averiguada utilizando o protocolo Organization for Economic Cooperation and Development (OECD ? 420/2001). A atividade antinociceptiva foi investigada nos testes do ?cido ac?tico, formalina e placa quente. O mecanismo de a??o foi apurado utilizando o antagonista opioide naloxona no modelo da placa quente. O estudo da atividade anti-inflamat?ria foi realizado utilizando os testes do edema de pata induzido por carragenina e da permeabilidade capilar induzida por ?cido ac?tico. Os resultados obtidos foram analisados atrav?s da an?lise de vari?ncia (ANOVA). Martianthus leucocephalus n?o mostrou toxidade aguda nem a??o miorrelaxante sobre o sistema motor. As propriedades antinociceptiva e anti-inflamat?ria foram evidenciadas de forma significativa em rela??o aos animais do grupo controle.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-10-02T00:07:17Z No. of bitstreams: 1 tese_ariana_reis_17_12_2014.pdf: 4645024 bytes, checksum: bcc726ad75f2d5883fb9e1118b2a9d3d (MD5) / Made available in DSpace on 2015-10-02T00:07:17Z (GMT). No. of bitstreams: 1 tese_ariana_reis_17_12_2014.pdf: 4645024 bytes, checksum: bcc726ad75f2d5883fb9e1118b2a9d3d (MD5) Previous issue date: 2014-12-17 / The aim of this study was to characterize the morphology, production, content, chemical composition and bioactive activity of essential oils of Lippia bromleyana, Lippia lasiocalycina, Lippia insignis and Lippia thymoides, endemic species of the Bahia semi-arid. The species were grown in the Experimental Station Horto Florestal State University of Feira de Santana (UEFS) in the city of Feira de Santana - Bahia. The essential oils are extracted from dry leaves and inflorescences, by hydrodistillation in Clevenger apparatus, and chemical composition was determined by GC/MS and GC/FID. Were carried out quantitative and qualitative morphological characterizations, in addition to agronomic characterization. The leaf anatomy, types and frequency of hair were observed in binocular microscope and electronic scanning light. The antioxidant activity was assessed by the ability of the substances present in the sample capture the free radical DPPH, using five concentrations of essential oils (2, 6, 10, 14 and 18 mg mL1) and the antifungal activity by mycelium growth in vitro testing five essential oil concentrations (0.25, 0.50, 0.75, 1.0 and 1.25 ?L mL-1). In conditions where the study was conducted, it can be concluded that there are morphological differences between species in all traits, except only the number of flowers per inflorescence; there agronomic differences for all traits with L. lasiocalycina stood out in relation to the variable oil yield, while L. insignis and L. thymoides regarding the essential oil content; were identified six types of glandular trichomes one, two and tetracelular and three types of trichomes; the species L. bromleyana presents as differential anatomical absence of trichomes on the abaxial surface; L. thymoides has glandular trichomes with irregular contours on both sides, distinguishing it from other species; the frequency of trichomes on the abaxial surface is higher in species L. insignis and L. lasiocalycina, which are more anatomically similar; the major compounds found in the samples of essential oils of L. bromleyana, L. lasiocalycina, L. insignis and L. thymoides were piperitone oxide and limonene; E-ocimenona, myrcenone, myrcene, ?-myrcene and ?-cymene; thymol, myrcenone and E-ocimenona; and ?-caryophyllene, germacrene D, respectively; L. insignis and L. bromleyana stood out in relation to the antioxidant and antifungal activity, respectively. / O objetivo desse trabalho foi caracterizar a morfologia e a produ??o, teor, composi??o qu?mica e atividade bioativa de ?leos essenciais de Lippia bromleyana, Lippia lasiocalycina, Lippia insignis e Lippia thymoides, esp?cies end?micas do semi?rido baiano. As esp?cies foram cultivadas na Unidade Experimental Horto Florestal da Universidade Estadual de Feira de Santana (UEFS), na cidade de Feira de Santana ? Bahia. Os ?leos essenciais foram extra?dos de folhas e infloresc?ncias secas, por meio da hidrodestila??o em aparelho de Clevenger e a composi??o qu?mica determinada por CG/EM e CG/DIC. Foram realizadas caracteriza??es morfol?gicas quantitativas e qualitativas, al?m da caracteriza??o agron?mica. A anatomia foliar, tipos e frequ?ncia de tricomas foram observados em microsc?pio de luz binocular e eletr?nico de varredura. A atividade antioxidante foi avaliada pela capacidade das subst?ncias presentes na amostra captarem o radical livre DPPH, utilizando cinco concentra??es dos ?leos essenciais (2, 6, 10, 14 e 18 mg mL-1) e a atividade antif?ngica pelo crescimento miceliano in vitro, testando cinco concentra??es do ?leo essencial (0,25, 0,50, 0,75, 1,0 e 1,25 ?L mL-1). Nas condi??es em que foi realizado o estudo, pode-se concluir que existem diferen?as morfol?gicas entre as esp?cies em todas as caracter?sticas avaliadas, com exce??o apenas para o n?mero de flores por infloresc?ncia; existem diferen?as agron?micas para todos os caracteres avaliados, sendo que L. lasiocalycina se destacou em rela??o ? vari?vel rendimento de ?leo, enquanto que L. insignis e L. thymoides em rela??o ao teor de ?leo essencial; foram identificados seis tipos de tricomas glandulares uni, bi e tetracelular e tr?s tipos de tricomas tectores; a esp?cie L. bromleyana apresenta como diferencial anat?mico aus?ncia de tricomas tectores na face abaxial; L. thymoides possui tricomas glandulares com contornos irregulares em ambas as faces, distinguindo-a das demais esp?cies; a frequ?ncia de tricomas tectores na face abaxial ? superior nas esp?cies L. insignis e L. lasiocalycina, as quais s?o mais semelhantes anatomicamente; os compostos majorit?rios encontrados nas amostras dos ?leos essenciais de L. bromleyana, L. lasiocalycina L. insignis e L. thymoides foram: ?xido de piperitona e limoneno; E-ocimenona, mircenona, mirceno, ?-mirceno e ?-cimeno; timol, mircenona e E-ocimenona; ?-cariofileno e germacreno D, respectivamente; L. insignis e L. bromleyana se destacaram em rela??o ? atividade antioxidante e antif?ngica, respectivamente.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-08-24T20:52:09Z No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) / Made available in DSpace on 2016-08-24T20:52:09Z (GMT). No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) Previous issue date: 2015-03-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Hyptis ramosa Pohl ex Benth (Lamiaceae) is native and endemic to the semi-arid northeast, with its unknown phytochemical constitution so far. Considering the pharmacological importance of the species of this family, the development of forms of propagation and in vitro culture can contribute to the inclusion of these species in sustainable production systems and the conservation of the same. Thus, the objective of this work was to study the in vitro propagation of the species H. ramosa, through direct organogenesis and callus formation, and the biochemical characterization of the obtained calluses, thus allowing the establishment of strategies for their conservation and sustainable use. To this, H. ramosa seeds were disinfected and established in medium MS / 2 culture. In the multiplication phase was tested the influence of cytokinins BAP, CIN and TDZ on different explants. The obtained shoots were individualized and transferred to MS / 2 medium containing different concentrations of auxin IBA and activated carbon for rooting them. Regenerated and rooted in vitro microplants were subjected to pre-acclimatization in different cultivation container closure and then were transferred to ex vitro conditions in commercial substrate Plantmax? being quantified plant survival rate at 30 days after transfer. For callus induction, we used explants and different concentrations of 2,4-D and BAP, determining the growth curve from the fresh weight of callus until the 28th day of cultivation, at intervals of seven days. Concurrent with obtaining the growth curve it is quantified in calluses obtained the total soluble sugar content, reducing sugar and crude protein. The in vitro propagation of H. ramosa is possible using the nodal segment as a source of explants in MS medium supplemented with BAP. In vitro rooting occurs, even in free auxin. The species showed survival of 100%, regardless of the day of pre-acclimatization phase. For callus induction the best explant is the nodal segment, and the combination of 2.4-D and BAP favor the formation of the same. The callus growth curve showed quadratic behavior with two different phases and biochemical analysis showed the maximum level of total soluble sugars, reducing sugars and crude protein at 14 ?, 21 ? and 14 ? days, respectively. / Hyptis ramosa Pohl ex Benth (Lamiaceae) ? uma esp?cie nativa e end?mica do semi?rido nordestino, sendo sua constitui??o fitoqu?mica desconhecida at? o momento. Considerando a import?ncia farmacol?gica das esp?cies dessa fam?lia, o desenvolvimento de formas de propaga??o e cultivo in vitro poder? contribuir para a inser??o dessas esp?cies em sistemas de produ??o sustent?veis e a conserva??o das mesmas. Diante disso, o objetivo deste trabalho foi estudar a propaga??o in vitro da esp?cie H. ramosa, atrav?s de organog?nese direta e calog?nese, bem como a caracteriza??o bioqu?mica dos calos obtidos, permitindo assim o estabelecimento de estrat?gias para a sua conserva??o e explora??o sustent?vel. Para isso, sementes de H. ramosa foram desinfestadas e estabelecidas em meio de cultura MS/2. Na fase de multiplica??o foi testada a influ?ncia das citocininas BAP, CIN e TDZ sobre diferentes explantes. As brota??es obtidas foram individualizadas e transferidas para meio MS/2 contendo diferentes concentra??es da auxina AIB e de carv?o ativo para o enraizamento das mesmas. As microplantas regeneradas e enraizadas in vitro foram submetidas ? pr?-aclimatiza??o em diferentes tipos de fechamento do recipiente de cultivo e, posteriormente, foram transferidas para a condi??o ex vitro em substrato comercial Plantmax?, sendo quantificada a taxa de sobreviv?ncia das plantas aos 30 dias ap?s a transfer?ncia. Para a indu??o de calos utilizou-se diferentes explantes e concentra??es de 2,4-D e BAP, determinando-se a curva de crescimento a partir da mat?ria fresca dos calos at? o 28o dia de cultivo, em intervalos de sete dias. Concomitante com a obten??o da curva de crescimento quantificou-se nos calos obtidos o teor de a??cares sol?veis totais, a??cares redutores e prote?na bruta. A propaga??o in vitro de H. ramosa ? poss?vel utilizando-se o segmento nodal como fonte de explante, em meio de cultura MS suplementado com BAP. O enraizamento in vitro ocorre, mesmo em meio isento de auxina. A esp?cie apresentou sobreviv?ncia de 100%, independentemente da realiza??o da fase de pr?-aclimatiza??o. Para indu??o de calos o melhor explante ? o segmento nodal, sendo a combina??o de 2.4-D e BAP favor?vel a forma??o dos mesmos. A curva de crescimento de calos mostrou comportamento quadr?tico com duas fases distintas e a an?lise bioqu?mica evidenciou o teor m?ximo de a??cares sol?veis totais, a??cares redutores e prote?na bruta aos 14?, 21? e 14? dias, respectivamente.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-27T00:25:31Z No. of bitstreams: 1 VERS?O FINAL DISSERTA??O-Luma Passos.pdf: 2866580 bytes, checksum: 631bf5534fde43f51e60719229aabc72 (MD5) / Made available in DSpace on 2016-09-27T00:25:31Z (GMT). No. of bitstreams: 1 VERS?O FINAL DISSERTA??O-Luma Passos.pdf: 2866580 bytes, checksum: 631bf5534fde43f51e60719229aabc72 (MD5) Previous issue date: 2015-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / The genus Lippia (Verbenaceae) is characterized by having species with medicinal and aromatic properties, its economic potential aims to the production of essential oils with important biological and pharmacological activities. Even with all known diversity and potential, few species are the target of agronomic studies. Considering the propagation as the first step in the domestication of a species and the influence of external factors on essential oil production, this study aimed to evaluate the effect of cutting type and auxin concentrations in the vegetative propagation of the species L. insignis, L. lasiocalycina and L. thymoides, as well as different types of fertilization and harvest season in production, and content and chemical composition of the essential oil. In propagation tests two types of cuttings were used (apical and middle) and five IBA concentrations (0, 250, 500, 750 and 1000 mg L-1). In cultivation three forms of fertilization were tested (control, manure and manure + NPK 10:10:10) and two harvest seasons (210 and 360 days after planting). The essential oils are extracted from dry leaves by the hydrodistillation method by Clevenger apparatus, and chemical composition determined by gas chromatography with flame ionization detector (GC / FID) coupled to mass spectrometer (GC / MS). It was concluded that apical cuttings are the most suitable for the propagation of L. insignis and both apical and middle cuttings for L. thymoides and L. lasiocalycina; treatment with IBA is not necessary in the propagation by cuttings of these species; chemical fertilizers (NPK) associated with cattle manure was the best treatment in growth and biomass production of the species L. lasiocalycina and L. thymoides, while for L. insignis to the control treatment was the best; pruning, followed by the second growing season (regrowth) is recommended in the cultivation of the studied species, aiming at the production of essential oil; ?-myrcene, limonene and E-ocimenone are the major components of L. insignis; ?-myrcene and E-ocimenone the major components of L. lasiocalycina and E-caryophyllene and caryophyllene oxide the major components of L. thymoides; fertilization and harvest season promote quantitative variations in the chemical composition of essential oils; the species L. insignis and L. thymoides are the most promising for essential oil production in the conditions of Feira de Santana, Bahia, Brazil. / O g?nero Lippia (Verbenaceae) ? caracterizado por apresentar esp?cies com propriedades medicinais e arom?ticas, sendo seu potencial econ?mico voltado para a produ??o de ?leos essenciais com importantes atividades biol?gicas e farmacol?gicas. Mesmo com toda diversidade e potencial conhecidos, poucas esp?cies est?o sendo alvo de estudos agron?micos. Considerando a propaga??o como o primeiro passo na domestica??o de uma esp?cie e a influ?ncia dos fatores externos na produ??o de ?leo essencial, o presente trabalho teve como objetivo avaliar o efeito do tipo de estaca e concentra??es de auxina na propaga??o vegetativa das esp?cies L. insignis, L. lasiocalycina e L. thymoides, bem como de diferentes tipos de aduba??o e ?pocas de colheita na produ??o, teor e composi??o qu?mica do ?leo essencial. Nos testes de propaga??o foram utilizados dois tipos de estacas (apical e mediana) e cinco concentra??es de AIB (0, 250, 500, 750 e 1000 mg L-1). No cultivo foram testadas tr?s formas de aduba??o (testemunha, esterco bovino e esterco + NPK 10:10:10) e duas ?pocas de colheita (210 e 360 dias ap?s o plantio). Os ?leos essenciais foram extra?dos de folhas secas pelo m?todo de hidrodestila??o por aparelho de Clevenger e a composi??o qu?mica determinada por cromatografia gasosa com detector de ioniza??o em chama (CG/DIC) acoplado a espectr?metro de massas (CG/EM). Foi poss?vel concluir que estacas apicais s?o as mais indicadas na propaga??o de L. insignis e tanto as estacas apicais quanto medianas para L. thymoides e L. lasiocalycina; o tratamento com AIB n?o ? necess?rio na propaga??o por estaquia dessas esp?cies; a aduba??o mineral (NPK) associado a esterco bovino foi o melhor tratamento no crescimento e produ??o de biomassa das esp?cies L. lasiocalycina e L. thymoides, j? para L. insignis o tratamento testemunha foi o melhor; a poda, seguida da segunda ?poca de cultivo (rebrota) ? recomend?vel no cultivo das esp?cies estudadas, visando ? produ??o de ?leo essencial; ?-mirceno, limoneno e E-ocimenona s?o os componentes majorit?rios de L. insignis; ?-mirceno e E-ocimenona os componentes majorit?rios de L. lasiocalycina e E-cariofileno e ?xido de cariofileno os componentes majorit?rios de L. thymoides; a aduba??o e a ?poca de colheita promovem varia??es quantitativas na composi??o qu?mica dos ?leos essenciais; as esp?cies L. insignis e L. thymoides s?o as mais promissoras na produ??o de ?leo essencial nas condi??es de Feira de Santana, Bahia, Brasil.

Les régions de Midi-Pyrénées (France) et de Chongqing (Chine) sont riches en plantes aromatiques et médicinales dites oubliées (ou médiévales). Afin de valoriser pleinement les différentes bio-molécules extractibles de ces plantes, le concept de MAP-raffinerie a été créé et appliqué à une sélection de plantes issues de ces deux régions. Plusieurs technologies d’extraction utilisant l’eau comme solvant vert (hydrodistillation, distillation à la vapeur et extraction par eau sub-critique) ont ainsi été employées et leur impact tant sur la composition des huiles essentielles que sur la récupération des molécules anti-oxydantes a été évalué. Dans un premier temps, une liste de plantes aromatiques et médicinales oubliées, voire sous-utilisées dans les deux régions a été établie selon des règles de sélection prédéfinies. Six plantes modèles de la région de Midi-Pyrénées (Tussilago farfara L., Calendula arvensis L., Robinia pseudoacacia L., Geranium robertianum L., Cytisus scoparius L. et Spartium junceum L.) et trois plantes de la région de Chongqing (Tussilago farfara L., Citrus aurantium L. et Saussurea costus) ont finalement été retenues. Puis, le concept de MAP-raffinerie a été appliqué à ces plantes afin d’étudier leur possible valorisation globale. L’étude des compositions chimiques des extraits volatils des racines de Tussilago farfara L. et de Calendula arvensis L., ainsi que des boutons de fleurs de Spartium junceum L. a été réalisée par GC et GC-MS pour la première fois. Les principaux composés chimiques dans l’extrait volatil de racines de Tussilago farfara L. étaient des hydrocarbures sesquiterpéniques et des composés aliphatiques tandis que les principaux composés chimiques dans l’extrait volatil de racines de Calendula arvensis L. étaient des sesquiterpènes oxygénés, des monoterpènes oxygénés et des diterpènes oxygénés. L’extrait volatil de boutons de fleurs de Spartium junceum L. était principalement composé de composés aliphatiques. Par ailleurs, les résultats de l’évaluation des capacités anti-oxydantes des extraits (par les tests DPPH, ABTS, FRAP, ORAC et Folin-Ciocalteu) ont montrés que plusieurs plantes comme Cytisus scoparius L., Tussilago farfara L., Citrus aurantium L. ou Robinia pseudoacacia L. pourraient être des sources potentielles d’anti-oxydants naturels. D’un point de vue technologique, les comparaisons de l’utilisation de l’hydrodistillation (HD), de la distillation à la vapeur (SD) et de l’extraction par eau sub-critique (SWE) ont montrées que si la HD et la SD ont des effets limités sur la composition des huiles essentielles, la HD semble être une méthode plus efficace pour la récupération des composés anti-oxydants à partir des résidus de distillation que la SD tandis que la SWE s’avère être une technologie prometteuse pour l’extraction directe de ces molécules à partir des plantes. Si la composition minérale de l’eau lors de l’hydrodistillation n’a que des effets très limités sur les rendements d’extraction, les teneurs en ions calcium et bicarbonate des eaux ont par contre des effets décroissants significatifs sur la capacité anti-oxydante et sur la teneur phénolique totale des extraits aqueux et méthanoliques. Au vue de ces résultats, un concept amélioré de MAP-raffinerie a été développé en intégrant une extraction à l’eau sub-critique pour l’extraction des composés anti-oxydants des résidus d’extraction primaire. Selon ce nouveau concept, cinq extraits peuvent être obtenus à partir des matières végétales: un extrait volatil, un extrait aqueux, un extrait méthanolique, un extrait à l’eau sub-critique et in fine un résidu solide. Les premiers résultats ont montrés que la "MAP-raffinerie améliorée" augmente de manière significative la récupération des antioxydants par rapport à la MAP-raffinerie originale et permet d’envisager une valorisation plus facile du résidu solide en agro-matériaux du fait de sa faible teneur en eau résiduelle. / In both Midi-Pyrénées region (France) and Chongqing region (China), there are rich and underutilized medicinal and aromatic plants (MAP). Aiming at fully exploiting different molecules in these plants, the concept of MAP-refinery was developed and applied to several underutilized medicinal and aromatic plants in these two regions. Several water-based green extraction technologies of natural products (e.g. hydrodistillation, steam distillation and subcritical water extraction) were also investigated to look at their effects on essential oil composition and antioxidants recovery from selected plants. Firstly, lists of forgotten or underutilized medicinal and aromatic plants in both regions were established according to the rules of selection. From the lists, six plants in the Midi-Pyrénées region (Tussilago farfara L., Calendula arvensis L., Robinia pseudoacacia L., Geranium robertianum L., Cytisus scoparius L. and Spartium junceum L.) and three plants in the Chongqing region (Tussilago farfara L., Citrum aurantium L. and Saussurea costus) were finally selected for investigations. Then the MAP-refinery was applied to the selected plants in two regions in order to realise their global valorisation. Volatile extracts composition in the roots of Tussilago farfara L. and Calendula arvensis L., as well as flower buds of Spartium junceum L. were firstly investigated. The main chemical compounds in volatile extract from Tussilago farfara L. roots were sesquiterpene hydrocarbons and aliphatic compounds while main chemical compounds in volatile extract from Calendula arvensis L. roots were oxygenated sesquiterpenes, oxygenated monoterpenes and oxygenated diterpenes. The volatile extract from flower buds of Spartium junceum L. was mainly composed of aliphatic compounds. Antioxidant capacity evaluation results (by DPPH, ABTS, FRAC, ORAC and Folin-Ciocalteu tests) showed that several plant samples like Cytisus scoparius L., Tussilago farfara L., Citrum aurantium L. and Robinia pseudoacacia L. could be potential sources of natural antioxidants. Comparisons of hydrodistillation (HD), steam distillation (SD) and subcritical water extraction (SWE) showed that HD and SD had limited effects on essential oil composition but HD, SD and SWE had significant impacts on the recovery of antioxidants. Hydrodistillation seemed to be a better method for recovery of antioxidant compounds from residues of distillation than steam distillation. However, SWE appeared to be a more efficient method for direct extraction of antioxidant molecules (or phenolic compounds) from plants. In the hydrodistillation process, mineral contents in water were found to have very limited effects on yields of extracts but calcium and bicarbonate ions, had significant decreasing effects on antioxidant capacity and total phenolic content of both aqueous and methanolic extracts. Finally, an improved MAP-refinery was developed. Subcritical water was used for further extraction of antioxidant compounds from residues in original MAP-refinery. In this way, five parts could be obtained from plant materials: volatile extract, aqueous extract, methanolic extract, subcritical water extract and the final residue. The results showed that the improved MAP-refinery significantly increased the recovery of antioxidants compared with original MAP-refinery. This promising process will also allow a better valorisation of the final solid residue due to the lower content of residual water.