Among the MEMS field, the design and simulation of piezoelectric micro-actuators are difficult as compared to thermal micro-actuators and electrostatic micro-actuators. The main reason of the piezo-electric material coupling effect is difficult to calculate. However the piezo-electric material has several advantages and characteristics for designing micro-actuators. Moreover, the design is usually done by the experimental or try-and-error method which is not so effective. It should be noted that there is not a simple method already developed for the design and simulation of the piezo-electric micro-actuators. In this research we proposed to use the software of ANSYS for the simulation of piezo-electric micro-pump. Simulation of force output of piezo-micro-pump can use ANSYS software to establish the simulation system of piezo-micro-pump. The micro-pump will have different resonance frequency, back-pressure and fluid due to piezo-electric characteristic. In this study, the author used a square, rectangle and circle geometric shape to simulate the result, each geometric shape has four different kinds of size. As a result, there are twelve groups of different simulation results. We are able to using the chart to present and explain the relation between resonance frequency and displacement.

This study presents a new process to fabricate gapless triangular micro-lens array (GTMA). The process includes optical simulation with tracepro, UV lithography, photoresist reflow process, Ni electroplating and hot embossing technique. After photoresist triangular column array is defined using UV lithography, reflow process is applied to melt photoresist triangular column array into the shape of triangular micro-lens array. With this reflowed triangular micro-lens array, Ni is deposited and covered uniformly on the triangular micro-lens array using electroplating. The growth rate of Ni is controlled at electroplating current density of 1 Ampere Square Decimeter (ASD; A/dm2). After this electroplating process is finished, a mold of GTMA is obtained, which is served as primary mold. Subsequently, with passivation technique applied on this mold¡¦s surface, electroplating process is applied again to obtain a secondary mold. Next, this secondary mold is served as master for the subsequent hot embossing process to replicate the GTMA pattern onto polymeric material PMMA and PET sheet. The mold with stiffness and hardness plays an important role in GTMA hot embossing process. In addition, this GTMA used as optical film can offer a 100 % fill factor and a simulation of optical coupling efficiency of 66.7% to improve luminance of backlight module (BLM). In addition, this study presents the fabricated molds of GTMA with different aspect-ratio about 0.109 and 0.133. The optical measurement of BLM shows that this optical film of GTMA pattern with aspect-ratio about 0.109 can increase 15.1% of luminance and with aspect-ratio about 0.133 can increase 22.1% of luminance.

A numerical simulation study for an interdigitated micro-channel PEM fuel cell is presented. Hydrogen gas is supplied to the anode and air is supplied to the cathode. The fuel cell outer surfaces are maintained at a constant temperature. The SIMPLEC algorithm is employed in a control volume numerical scheme. The outflow boundary conditions are specified to all transport equations except that an outlet pressure is specified to the momentum equation. Results are compared and show good agreement with the experimental data. The effects of the mass flow rate, the outlet pressure and the cell surface temperature on the cell performance are studied. The results can provide reference for fuel cell design.

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Orientador: André Rodrigues / Coorientador: Henrique Ferreira / Banca: Simone Possedente de Lira / Banca: Lara Durães Sette / Resumo: As coleções de culturas são importantes pilares na preservação, catalogação e prospecção da biodiversidade microbiana. Com o propósito de atender ao depósito e fornecimento de material biológico de qualidade, a Central de Recursos Microbianos da UNESP (CRM-UNESP) iniciou sua adequação para alcançar os padrões internacionais de operação e gerenciamento; o que inclui a autenticação taxonômica das culturas mantidas na coleção. Utilizando um método de triagem molecular adaptado nesse estudo (microssatélites), aliado ao uso de marcadores morfológicos e moleculares, realizamos a identificação taxonômica de 189 isolados, de um total de 201 fungos filamentosos selecionados com base na diversidade de fontes de isolamento e mantidos no acervo de pesquisa da CRM-UNESP. Com o intuito de agregar informações sobre o potencial biotecnológico desses isolados, também avaliamos a produção de compostos antimicrobianos. Após a reativação e purificação dos isolados, foram identificados 66 gêneros e 116 espécies, sendo que os gêneros mais abundantes foram: Fusarium (18,9%), Clonostachys (11,4%), Trichoderma (7%) e Penicillium (6,5%). Todos os 201 isolados foram avaliados quanto à produção de metabólitos secundários com propriedade antibacteriana e antifúngica, segundo um método de alto desempenho (também adaptado neste estudo), frente a micro-organismos de interesse agrícola (Xanthomonas citri subsp. citri) e clínico (Staphylococcus aureus, Escherichia coli, Candida albicans, Candida krusei e Candida parapsilosis). Do total dos cultivos brutos avaliados, a maioria (62,7%) apresentou atividade antagonista frente, a pelo menos, um dos micro-organismos de referência. Os cultivos brutos dos fungos Penicillium aurantiogriseum LESF 194, Clonostachys rosea LESF 389, Penicillium dodgei LESF 487, Xylaria sp. 4 LESF 502 e de um isolado não identificado LESF 227 inibiram o crescimento de todos os micro-organismos de... / Abstract: Culture collections are keystones in the preservation, cataloging and exploration of microbial diversity. In order to meet international standards of deposit and supply of biological materials, the UNESP-Microbial Resources Center (CRM-UNESP) started their suitability for functioning and management to reach international standards; which include taxonomic authentication of cultures preserved in the collection. Using an adapted molecular screening protocol (microsatellites) coupled with morphological and molecular markers, we obtained the taxonomic identification of 189 out of 201 filamentous fungi from the CRM-UNESP research collection. In order to gather information on the biotechnological potential of these isolates, we also evaluated the production of antimicrobial compounds. After revival and purification of fungal isolates, we identified 66 genera and 116 species; comprehending the most abundant genera: Fusarium (18.9%), Clonostachys (11.4%), Trichoderma (7%) and Penicillium (6.5%). All isolates were evaluated for the production of secondary metabolites with antibacterial and antifungal properties, according to a high-throughput method (also adapted in this study), towards agricultural (Xanthomonas citri subsp. citri) and clinical-relevant (Staphylococcus aureus, Escherichia coli, Candida albicans, Candida krusei and Candida parapsilosis) reference strains. The majority of culture broths (62.7%) showed antagonist activity against at least one of the reference strains. Culture broths of Penicillium aurantiogriseum LESF 194, Clonostachys rosea LESF 389, Penicillium dodgei LESF 487, Xylaria sp. 4 LESF 502 and from the non-identified fungus LESF 227 inhibited the growth of all reference strains. Our results contributed for the establishment of two screening approaches in the routine of CRM-UNESP. These methods allowed the identification of the various fungal isolates examined as well as the evaluation of the antimicrobial potential of ... / Mestre

Micro-electro-mechanical systems (MEMS) became possible thanks to the silicon based technology used to fabricate integrated circuits. Originally, MEMS fabrication was limited to silicon based techniques and materials, but the expansion of MEMS applications brought the need of a wider catalog of materials, including polymers, now being used to fabricate MEMS. Polyimide is a very attractive polymer for MEMS fabrication due to its high temperature stability compared to other polymers, low coefficient of thermal expansion, low film stress and low cost. The goal of this thesis is to expand the Polyimide usage as structural material for MEMS by the development of a multi-user fabrication process for the integration of this polymer along with multiple metal layers on a silicon substrate. The process also integrates amorphous silicon as sacrificial layer to create free-standing structures. Dry etching is used to release the devices and avoid stiction phenomena. The developed process is used to fabricate platforms for micro-hotplate gas sensors. The fabrication steps for the platforms are described in detail, explaining the process specifics and capabilities. An initial testing of the micro-hotplate is presented. As the process was also used as educational tool, some designs made by students and fabricated with the Polyimide-MEMS process are also presented.

No description available.

This thesis reports on the work performed on the manipulation of micro­-particles on substrate surfaces using short laser pulses. For particles with diameters on the order of microns, the binding forces to surfaces are significantly larger than gravitational forces. To overcome these binding forces and manipulate the particles the use of femtosecond laser pulses has been investigated. Individual micro-particles (poly-divinylbenzene, glass and silver materials) with diameters around 2 um were removed from substrate surfaces (dielectric, semiconductor and metal substrates) us­ing a Ti:Sapphire laser system. The pulses produced at 800 nm had pulse lengths around 140 fs and were tightly focussed onto the surface using 5x and 10x micro­scope objectives. The peak fluence thresholds for particle removal were determined and the surfaces examined after irradiation by a scanning electron microscope and atomic force microscope to check for damage. The experimental results indicate that ablation of the substrate below the micro-particles is most likely to be respon­sible for micro-particle removal from the substrate surface when using femtosecond pulses. Ablation pits were observed for the dielectric micro-spheres on semiconductor substrates. It is also believed that ablation is responsible for the removal of other types of micro-particles from various substrates. Unlike the dielectric micro-sphere on semiconductor substrate results, the other particle-substrate combinations show a close correspondence between the removal and substrate ablation thresholds. It is believed that these results indicate the occurrence of ablation leading to the removal of the micro-particles. Calculations of the local electromagnetic fields around the micro-particles have also been carried out and the distributions used to interpret the experimental results. / Thesis / Master of Applied Science (MASc)

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