Malgré les progrès technologiques modernes, l'industrie du vêtement est toujours ancrée dans une approche traditionnelle en 2D et en 3D lors d’essayage virtuel. La question fondamentale de l’accessibilité aux données morphologiques du consommateur en ligne n'a toujours pas été résolue de manière appropriée. De plus, l’interactivité et le relationnel entre le corps humain et le vêtement ne sont pas suffisamment explorés pour atteindre une performance satisfaisante lors de l'essayage du vêtement en ligne. Mes travaux de recherche ont donc pour objectif de combler ces lacunes en proposant une plate-forme numérique intégrant à la fois la connaissance des experts du secteur de l’habillement (ajustement et confort), et les retours sensoriels des clients (au porté du produit) en misant en place une nouvelle stratégie de conception de vêtement en 3D afin de calculer et d’ajuster les valeurs de l’aisance 3D de celui-ci, comme les points clefs lors de la perception et la satisfaction du produit par le client. C’est à partir d’une méthode de classification supervisée associée à un descripteur de forme 2D que nous avons retrouvé le morphotype du client en 3D avec son avatar. Cette relation complexe entre la reconnaissance du corps porteurs et la conception de vêtements 3D approprié dans essayage virtuel a été testée et analysée dans le cadre de ce projet pour bâtir une solution de conception adaptée à un environnement à distance. À cette fin, nous avons présenté les principes de la modélisation du vêtement directement adaptés à la morphologie du porteur afin de couvrir toute la gamme de formes et de mesures corporelles. / Despite modern technological progresses, the apparel industry is still anchored in the traditional 2D-to-3D design approach. Additionally, the aspects of the relation between human body and garment are not sufficiently explored in order to provide satisfactory performance of virtual try-on in the aspects of providing not only right fit and comfort to the customer but also avoiding returns to the retailer. However the main aspect is a lack of appropriately resolved issue of consumers’ body recognition in an online environment and proper 3D design methodology for individual client. In my PhD research, we challenge those gaps by proposing a foundation of a digital and knowledge-based platform for garment design and fit and comfort evaluation by integrating customers' and experts’ knowledge with the design parameters. By building a new 3D design strategy, we proposed an original method to calculate and adjust the 3D ease allowance values, which constitutes the key issues of satisfaction perception. Our 3D design method is linked to the consumer’s virtual representation, which come from a new pattern recognition method permitting to identify individual morphology from a single web-camera. It was experimentally shown that using the supervised method to create 2D shape descriptors enables to detect wearers’ morphotypes for a target population. The complex relationship between wearers’ body recognition, 3D garment design and garment fitting in virtual try-on has been tested and analyzed in the scope of this research project to build a suitable design solution applied to the remote environment.

Toxigenic Aspergillus species produce mycotoxins that are carcinogenic, hepatotoxic and teratogenic immunosuppressing agents in both human and animals. Kenya frequently experiences outbreaks of aflatoxicosis with the worst occurring in 2004, which resulted in 125 deaths. This study sought to find possible reasons for frequent aflatoxicosis outbreaks in Kenya by isolating Aspergillus flavus strains from maize kernels sampled from different climatic regions of Kenya. Using diagonal transect random sampling, maize kernels were collected from Makueni, Homa Bay, Nandi, and Kisumu regions. The genetic diversity and variation among the isolates was examined by characterising the strains according to morphology, phenotype, vegetative compatible groups and molecular systematics. Selected atoxigenic and aflatoxigenic A. flavus isolates were also further analysed for aflatoxin production potential using quantitative real-time PCR and various bioanalytical techniques. The influence of the maize lines grown in Kisumu, Homa Bay, Nandi and Makueni region on A. flavus infection and aflatoxin production was also examined and served as the basis for an in vitro biocontrol assay. Out of 37 isolates identified, nitrate non-utilizing auxotroph’s complementation test revealed 20 vegetative compatibility groups. These groups were further designated using the prefix ʻʻKVCGʼʼ, where ʻʻKʼʼ represented Kenya and consequently assigned numbers 1 to 20 based on our findings. KVCG14 and KVCG15 had highest distribution frequency (n = 13; 10.8 %). The distribution of the L, S and S/L- morphotypes across the regions were 57 % (n = 21); 7 % (n = 3) and 36 % (n = 13) respectively. The phylogenetic analysis exhibited high diversity of A. flavus isolates from Makueni. ITS1 and ITS2 markers did not reveal significant information within intraspecies speciation of A. flavus. Furthermore, a unique isolate (KSM015) was identified that had characteristics of S-morphotype, but produced both aflatoxins B and G. Coconut agar medium (CAM) assay, TLC, HPLC and LCMS/MS analyses confirmed the presence or absence of aflatoxins in selected toxigenic and atoxigenic isolates. qPCR analysis revealed aflP, aflS, aflR and aflO transcripts as the most upregulated genes across the tested isolates whereas false detection of aflD gene transcript was observed in both induced and uninduced A. flavus isolates. Diversity Index (H) analyses ranged from 0.11 (Nandi samples) to 0.32 (Kisumu samples). Heterokaryon compatibility ranged from 33 % (for the Makueni samples, n = 3) to 67 % (Nandi samples, n = 6). The KDV1 maize line was more sensitive to A. flavus infection in comparison to GAF4. We also tested the biocontrol of atoxigenic isolates to inhibit toxin production by aflatoxigenic strains on infected maize kernels. It was shown that the atoxigenic strain (KSMO12) could inhibit the aflatoxigenic strain (KSM014) depending on the atoxigenic concentration during infection. To our knowledge, this is the first reported study for A. flavus genetic diversity, variation and distribution in Nandi, Homa Bay and Kisumu regions in comparison to and could assist researchers in the selection of biocontrol strategies to mitigate aflatoxin contamination, especially in Makueni and neighbouring regions.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19756号 / 農博第2152号 / 新制||農||1038(附属図書館) / 学位論文||H28||N4972(農学部図書室) / 32792 / 京都大学大学院農学研究科応用生物科学専攻 / (主査)准教授 田川 正朋, 教授 荒井 修亮, 教授 山下 洋 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

Lors des accidents de la route, les lésions de l’abdomen mettent des vies en jeu et nécessitent un traitement médical long et coûteux. Il est important d’offrir une meilleure prévention des lésions abdominales traumatiques. Des modèles numériques de corps humain ont été développés afin de comprendre les mécanismes lésionnels. L’objectif de cette thèse est de contribuer au développement d’un modèle numérique de foie humain pour la prédiction de lésions hépatiques en cas de choc.Dans un premier temps, une caractérisation morphologique du foie a été réalisée sur 78 scanners et a mis en évidence l’existence de 4 morphotypes. Ensuite, des expérimentations ont mis en évidence l’influence de la pressurisation des vaisseaux sur la déformation de la capsule et ont permis d’identifier la déformation à rupture locale lors d’un choc.Enfin, les modèles numériques des 4 morphotypes identifiés ont été construits pour simuler les essais de décélération réalisés dans la partie expérimentale. / In road accidents, lesions of the abdomen are life-threatening, and require a long and expensive medical treatment. It is important to offer a better prevention of traumatic abdominal injuries. Numerical models of the human body have been developed to understand the mechanisms of injury. The aim of this thesis is to contribute to the development of a numerical model of human liver for the prediction of liver lesions in case of shock.First, a morphological characterization of the liver was performed on 78 healthy patient scanners and highlight the existence of 4 morphotypes.Then, experiments were carried out to highlight the influence of this pressurization of the vessels on the strain state of the Glisson capsule and to identify the local ultimate strain during an impact.Finally, the numerical models of the 4 identified morphotypes were constructed and used to simulate the deceleration tests performed in the experimental part.

L'utilisation de modèles en éléments finis du corps humain a été initiée au début des années 90 comme outil d'aide à la conception de dispositifs de sécurité automobile. Obtenir une personnalisation géométrique de tels modèles est une préoccupation récente afin d'en optimiser la biofidélité. Le but de cette étude est donc d'accroître notre compréhension des géométries externes et internes du corps humain, et fournir des outils de personnalisation de modèles numériques. En premier lieu, une base de données géométriques externes (anthropométrie corps entier) et internes(morphométrie du rachis, du bassin et du thorax) collectées sur 85 volontaires a été constituée à l'aide de mesures anthropométriques classiques et des méthodes de reconstruction stéréoradiographiques tridimensionnelles. L'exploitation de cette base de données a abouti à une description détaillée des géométries externes et internes de sujets asymptomatiques. Elle a également permis de développer et évaluer une méthode statistique d'estimation de paramètres anthropométriques (externes) et morphométriques (internes) basée sur près de 200 modèles anthropométriques (i.e. régressions linéaires simples et multiples) externe/externe et externe/interne. Grâce à cette méthode, 10 mesures anthropométriques suffisent pour modéliser la géométrie externe (43 dimensions du corps entier assis et debout) et interne (155 dimensions du bassin, vertèbres C3 à L5, et côtes niveaux 1 à 10) d'un individu (errreur moyenne: 2,3%(2

The effect of sub-lethal chlorine stress on various strains/serotypes of Salmonella on biofilm formation and thermal resistance was studied. The effect of oxidative stress (induced by 150 ppm of chlorine in TSB) on Salmonella biofilm formation on polystyrene and stainless steel surfaces at three temperatures (4°C, 30°C, and room temperature) in nutrient rich (full strength TSB) and nutrient limited conditions (1/10th TSB) was evaluated. On polystyrene surface, chlorine stressed S. Heidelberg (strain ID 72), S. Newport (strain ID 107) and S. Typhimurium (ATCC 14028) formed stronger (P < 0.05) biofilms at 30°C. On stainless steel, the chlorine stressed S. Heidelberg (ATCC 8326) and S. Enteritidis (ATCC 4931) at room temperature formed stronger (P < 0.05) biofilms as compared to the non-stressed control cells. The thermal resistance of short-term (1h) and long-term (27d) chlorine stressed Salmonella Heidelberg and S. Typhimurium were compared with the non-stressed controls at three different temperatures (55°C, 58°C and 61°C) and two growth phases (logarithmic and stationary). The short-term stressed log phase cells (both serotypes) were found to be more sensitive (P< 0.05) to thermal inactivation in TSB. Upon long-term sub-lethal chlorine exposure, Salmonella developed a rugose morphotype on tryptic soy agar at 37°C. The rugose morphotype provided significant thermal protection (P< 0.05) against heat stress as compared to smooth morphotype. In chicken broth, at 55°C, short-term chlorine stressed stationary phase S. Typhimurium displayed a higher D55 value compared to non-stressed cells. The findings from this research reveal that some Salmonella strains have the potential to form stronger biofilms and exhibit higher thermal tolerance upon exposure to sub-lethal chlorine concentration.

Abstract Mycorrhizal fungi are important contributors to the functioning of boreal forests, since they act in the bilateral carbon and nutrient transport between above- and belowground parts of the ecosystem. In ectomycorrhizal (ECM) symbiosis of woody host plants, both fungal and plant partners depend on resources provided by the other. A single tree may simultaneously host several ECM fungal partners, which greatly enhance the host's nutrient uptake. At the same time nearly 20% of host primary production is allocated to mycorrhizal fungi. Although fungi depend on host-derived carbon, it is poorly understood how reduced carbon availability, e.g., due to herbivory, affects the ECM fungal symbionts. In this thesis I studied the impact of simulated insect defoliation or mammal browsing on mycorrhizal fungi of boreal woody hosts. Quantitative and qualitative changes in biomass partitioning in different fungal compartments were detected. None of the experiments showed that defoliation or shoot clipping treatments reduced the intensity of ECM colonisation, while treatments often shifted fungal composition towards less biomass producing ECM morphotypes. Above- and belowground diversity in ECM symbionts tended to decrease due to shoot or foliar damage. In addition, in some cases defoliation also reduced fungal biomass in fine roots and decreased ECM sexual reproduction by reducing the number of sporocarps produced. Defoliation induced a similar response pattern in the host and in ECM fungi with a stronger response to increasing severity of treatment (e.g. degree of removed foliage or repeated years of defoliation). This was also confirmed when relating the effects of host and ECM fungal symbionts to defoliation using present and previously published data. The present results suggest that belowground adaptation of boreal trees to the changing environment is mediated by changes in fungal community or biomass partitioning. The lack of response in the intensity of ECM colonisation further emphasises the importance of the symbiosis to boreal trees.

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