This diploma thesis is focused on determination of bioavailable cadmium in natural waters. Thesis is devided to two parts-laboratory study in model solution and experiments in real surface water system. During the laboratory experiments the accumulation and release constants of cadmium by aquatic moss Fontinalis antipyretica were determined. In Morava river experiment this aquatic moss was used to determine bioavailable fraction of cadmium and the moss bag technique were compared with another methods for phytotoxic metals determination.

Flea beetles (Alticini) are a highly diversified group of leaf beetles (Chrysomelidae) with about 8 000 known species from about 560 genera distributed worldwide except Antarctica. The major life strategy of flea beetles is external feeding on vascular plants, similarly to other leaf beetles. However, studies published during last years revealed existence of numerous different flea beetle genera feeding on mosses. Moss-inhabiting flea beetles are usually highly specialized and share similar morphological characteristics, including flightlessness, compact body shape and modified antennae. However, their phylogenetic position remained unknown. In this study, I performed a phylogenetic analysis of 14 known moss- and leaf litter inhabiting flea beetle genera, included into a large dataset of various genera of flea beetles. I sequenced 2 mitochondrial and 2 nuclear genes previously used for phylogenetic analyses of Alticini. I also added numerous Neotropical external feeding alticine genera, because taxon samples from previous studies (Ge et al. 2012; Nie et al. 2017) consisted mainly of Oriental genera. Although deep divergences and phylogenetic positions of several genera were not resolved, the analysis revealed a multiple origin of moss- and leaf litter inhabitance among flea beetles. The morphologically...

Anandamide, N-arachidonylethanolamide (NAE 20:4), is an endocannabinoid receptor ligand unique to animals, in which it influences a wide range of physiological and behavioral functions. Using selective lipidomics approach, we recently identified occurrence of anandamide or NAE 20:4 and its precursor in moss plants. While Nacylethanolamines (NAEs) with C12-C18 acyl chain are ubiquitous in seed plants and play a role in mediating abscisic acid (ABA)-dependent or -independent responses to stress, endocannabinoid receptor-mediated interactions, similar to that of animals, have not been elucidated for plants. Physcomitrella patens provides us with a unique opportunity to address if 1) early land plants, such as mosses, retained NAE-mediated signaling mechanism that is akin to animals but not to vascular plants and 2) if such distinctive NAE profile and mechanisms by which it may function in moss plants is responsible, in part, for their natural ability to resist high temperatures and tolerate osmotic and salt stresses and dehydration. Our current studies are focused on characterization of anandamide metabolic pathway and its functional role in the development of moss. Insights into unique lipid composition and signaling pathways that mosses acquired naturally, during their successful transition from water to land, may lead to development of tools necessary to enhance abiotic stress tolerance in other plants.

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Source code plagiarism is an ongoing problem in programming courses at higher academic institutions. For this reason, different automated source code plagiarism detection tools have been developed. However, they require several manual steps before the submissions can be compared. Linnaeus University uses GitLab to handle their students’ code-related assignments but lacks an integrated workflow for checking submissions against plagiarism. Instead, Linnaeus University’s plagiarism-checking process is done manually, which is a challenging and time-consuming process. This thesis is a case study on Linnaeus University, focusing on integrating one of the plagiarism detection tools with GitLab using Continuous integration pipelines. The objectives have been to collect students’ submissions, communicate with the plagiarism tool, and visually present the results within GitLab. The prototype has been evaluated by a set of manually created submissions with different levels of plagiarism to ensure that the detection tool differentiates plagiarized and non-plagiarized submissions. Teachers at Linnaeus University have tested the workflow and reasoned whether the prototype fulfills their requirements.

Annual bluegrass (Poa annua L.) is the most problematic weed to control in creeping bentgrass (Agrostis stolonifera L.) putting greens.  The objective of this study was to transition a mixed putting green stand of annual bluegrass and creeping bentgrass to a monoculture by using fertilizers and plant growth regulators that selectively inhibit annual bluegrass.  A 25 year old loamy sand rootzone research green, planted with \'Penn-Eagle\' creeping bentgrass, with roughly 45% initial annual bluegrass coverage was utilized.  The biweekly application of ammonium sulfate (4.8 kg ha-1) with treatments of ferrous sulfate at rates of 0, 12.2, 24.4, and 48.8 kg ha-1 and in combination with seaweed extract (12.8 L ha-1) or paclobutrazol (0.37 L ai ha-1 spring and fall; 0.18 L ai ha-1 summer) were applied March to October, 2011 and 2012.  Plots receiving the highest rate of ferrous sulfate resulted in annual bluegrass infestation declines from an early trial amount of 45% to a final average of 20% but also resulted in unacceptable late-summer events of annual bluegrass collapse.  The ferrous sulfate medium rate resulted in a smooth transition from early-trial annual bluegrass infestation of 45% to an end of trial infestation of 20% and had the highest putting green quality.  Previous research has reported that consistent use of paclobutrazol can effectively and safely reduce annual bluegrass infestations.  In this trial annual bluegrass was reduced to 9% infestation after three months of application.  Two unexpected observations from this trial were that ferrous sulfate, applied at medium to high rates, significantly reduced silvery thread moss (Bryum argentum Hedw.) populations and occurrences of dollar spot (Sclerotinia homoeocarpa F. T. Bennett) disease.  Dollar spot control with ferrous sulfate has not previously been reported in the literature, so additional studies were designed to investigate this phenomenon further.  A creeping bentgrass putting green study was conducted to determine if sulfur, iron, or the two combined as ferrous sulfate decreases dollar spot activity.  Ferrous sulfate resulted in the highest turf quality and suppressed S. homoeocarpa infection, even during high disease pressure.  Fe-EDTA suppressed dollar spot infection as well as ferrous sulfate but quality declined to unacceptable levels during the summer, due to Fe-EDTA only.  Sulfur did not affect or increased S. homoeocarpa infection, indicating that a high and frequent foliar rate of iron is responsible for dollar spot control.  An in-vitro study was conducted to determine if agar pH in combination with iron concentrations affects mycelial growth of S. homoeocarpa.  Results from this trial indicated that 5.4 agar pH is an optimal pH for mycelial growth.  The 10 to 100 mg iron kg-1 concentration had little effect on mycelial growth at 5.0 and 5.5 pH, but increased growth at 4.5 and 6.5 pH.  As the iron concentration was increased from 10 to 100 to 1000 mg kg-1, mycelial growth decreased or stopped.  Our final conclusions are that seasonal biweekly foliar applications of the medium rate of ferrous sulfate (24.4 kg ha-1) safely and effectively reduced annual bluegrass infestation out of a creeping bentgrass putting green, while also effectively suppressing silvery thread moss and dollar spot incidence. / Master of Science

My thesis is a presentation of Jika Laufen for the international exhibition Expo 2015 in Milan. The work consists of designing and installing sinks into the exposure so that they are in a uniform manner. I designed the concept of the well for several reasons: the bathroom is an intimate space where it should be emphasized emotion , atmosphere and feelings. It is a space where a person should relax. The second aspect is that the ceramic has an organic origin, came from the ground and through the fire becomes very durable and solid. A no less important is the third aspect, exist on the context. Expo is an international exhibition and therefore the presentation of this forest exposure does not represent a Czech company, but also a Czech environment, where you will find beautiful nature and sources of known rivers. My concept is based on emotion and contrast materials. Delicate white ceramics have planted in a sharp stone and dark green leafy moss, creating a contrast full voltage . The exhibition of this type should not be missed even modern technology. That is my proposal represented feeler drain, which is around the perimeter of the sink hidden under the edge so as not to disturb the perception of the whole.

Formin proteins, important regulators of a cell's actin cytoskeleton, nucleate actin polymerization and promote filament elongation. Actin dynamics are crucial for a form of polarized growth termed tip growth that is performed by cells involved in reproduction and nutrient uptake in plants. Uncovering the molecular basis of how actin associated proteins like formins control actin dynamics is important to gain a fundamental understanding of plant growth mechanisms. In the moss Physcomitrella patens, there are 9 formin genes that group into three distinct classes (I, II and III). From previous work, we suspect that class I formins may play a role in cytokinesis. Thus, I investigated how class I formins localize in tip-growing protonemal cells to gain further insight into their function. To do this, I tagged class I formins with GFP at the endogenous locus and visualized their subcellular localization using confocal microscopy. I found that Formin 1A, 1D, 1E and 1F localize to punctate spots on the plasma membrane and may concentrate at the cell plate during cell division, while 1B localizes to the cytosol. Overall, these data have shown that class I formins may play a role in cell division and potentially in the secretory pathway.

Northern peatlands have acted as persistent sinks of CO2 throughout the Holocene largely owing to their ability to maintain shallow water table depths that limit decomposition rates and supports the growth of keystone vegetation including Sphagnum mosses. There is concern, however, that the future success and ecosystem function of these northern peat deposits may be at risk to climate change, where temperatures and evaporation rates are predicted to increase substantially in the next century. While numerous studies have examined the hydrology and carbon dynamics in large expansive peatland systems where a water table (WT) is ever-present, relatively little research has been done on small scale peat-accumulating systems where their vulnerability remains unknown. One region where a broad spectrum in the scale of peat accumulation is present is in the bedrock depressions of Canadian Shield rock barrens, which are of special importance as many peat deposits here provide habitat to species at risk including the Blanding’s Turtle and the Massassauga Rattlesnake. This study examines the controls that govern water storage dynamics and moss water availability in 18 different peat-accumulating depressions that vary in size, catchment area, and sediment composition. The magnitude of WT variability was often several times greater in shallower bedrock depressions (<50 cm deep) as compared to deeper ‘bogs’ (>60 cm deep). The magnitude of depression WT variability appeared to be closely linked to the WT depth (WTD), the relative proportions of different sediment types within the depression, and the depth dependant specific yield (Sy) of each sediment type. Sites which contained large fractions of Polytrichum moss or mineral soil – which were more common in shallow depressions ¬¬– had the greatest WT variability due to the lower porosity and Sy of this sediment as compared to Sphagnum peat. Sphagnum dominated ‘vernal pools’ (30-50 cm deep) had a WT variability two to three times greater than Sphagnum dominated bogs at WTDs > 20-25 cm, which may be related to exceptionally high ash concentrations near the base of vernal pools which reduced peat porosity and Sy as compared to more organic-rich peat. As compared to bogs, pits (<15 cm deep) and vernal pools had greater rates of WT decline during drying intervals, deeper average WTDs when a WT was present, and extended periods of WT absence during the summer months. As such, moss growing in pits and vernal pools generally had lower near-surface water availability as compared to bogs, though the importance of depression depth in determining the timing of moss stress is also dependant on the hydrophysical properties (Kunsat and moisture retention) of the moss species in question. WT dynamics and moss water availability were generally weakly correlated to depression catchment size, although during wetter periods of the year the rate of WT recession was moderated in pits and vernal pools which had an upslope depression that could provide sustained water inputs for multiple days after rainfall. The results of this study suggest that depression depth may be a first order control in determining peatland vulnerability to future regime shifts induced by external forcings or disturbances. Furthermore, this study suggests that systematic differences may exist between the hydrophysical properties of peat in shallow vs. large bedrock depressions, potentially resulting from contrasts in fire frequency/severity, and/or the degree of humification/compression among geological settings. / Thesis / Master of Science (MSc) / Canada is home to one of the largest reservoirs of organic carbon stored on land in the world, in unique ecosystems called peatlands. Peatlands are formed in wetland environments where a thick layer of organic matter has accumulated over time due to the average rate of vegetation growth on the surface of peatlands exceeding the rate of decomposition of the underlying organic matter. This net accumulation of organic matter over time has caused peatlands to act as a long term sink of carbon dioxide, which is a greenhouse gas that is a primary driver of global warming. The ability of peatlands to have slow decomposition rates and support the growth of key peatland vegetation, most notably various species of ‘peat moss’, is highly dependent upon their ability to keep their water table (i.e. the surface below which pore spaces in the organic matter are saturated with water) close to their growing surface. There is concern, however, that a warmer and dryer climate in the future could cause deeper water table positions in peatlands, thereby increasing decomposition rates, decreasing the growth rate of peat moss, and potentially turning peatlands into a net source of carbon dioxide. Most peatland studies to date, however, have focused on water storage/movement and carbon exchange in large, deep peatland systems, whereas relatively little research has been conducted on smaller peatlands. As such, the vulnerability of these smaller peatlands to future climate change remains uncertain. One region where peatlands exist over a wide range of different sizes and landscape positions is in bedrock depressions of the Canadian Shield, which are of special interest as they also provide habitat for species at risk including the Blanding’s Turtle and the Massassauga Rattlesnake. This study looked at how the water table positions and water availability to different species of peat moss compared over the growing season between 18 peatlands of different sizes and landscape position (i.e. peatlands with a relatively ‘small’ and ‘large’ area upslope of them). This study finds that deeper peatlands (with organic matter layers > 60 cm deep) usually had a shallower water table over the summer months than shallower peatlands (< 50 cm deep), primarily due to differences in the properties of the organic matter underlying their growing surfaces. Furthermore, each of the 12 studied peatlands < 50 cm deep lost their water table for a considerable amount of time during the summer (when their water table position dropped below the underlying bedrock of the depression), whereas each of the six peatlands > 60 cm deep had a water table present for the entire growing season. Surprisingly, a peatland’s position on the landscape seemed to have a relatively minor effect on determining the depth/presence of its water table. As deeper peatlands usually had a water table that was closer to the growing surface and was always present, more moisture was available to the peat moss growing at their surface than for peat moss in shallower depressions, though this moisture availability also depended upon the growth form of the different species of peat moss (some species of peat moss were better at accessing subsurface water than others). Through its impact on water table positions and moisture availability for peat moss, peatland depth is likely a primary control governing peatland vulnerability climate change, with shallower peatlands being more vulnerable to warmer and dryer conditions in the future.

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