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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Inhibitor studies of normal and abnormal sporocarp development in the mushroom Collybia velutipes /

Long, Terrill Jewett January 1961 (has links)
No description available.
22

Produção de cogumelos comestíveis e fenol-oxidases de Pleurotus sajor-caju e Lentinula edodes em resíduos lignocelulósicos

Finimundi, Jane Maria 11 November 2011 (has links)
Cogumelos comestíveis de Pleurotus sajor-caju PS-2001 e Lentinula edodes LE-01 foram cultivados em substratos constituídos de serragem de Pinus sp ( para P. sajor-caju) ou Eucalyptus sp (para L. edodes) adicionados de resíduos de bagaços de Vitis labrusca e Malus domestica. Foram avaliados os parâmetros de produção produtividade (P), eficiência biológica e rendimento (R), atividade das enzimas (lacases, VAO, MnP and LiP), proteína solúvel total e a N-acetil glicosamina liberada do micélio como uma estimativa indireta do crescimento da biomassa. Os testes foram realizados com os bagaços dos dois frutos separadamente e em serragem de Pinus sp. (para P. sajor-caju) e Eucalyptus sp.. Foram utilizadas três combinações de substratos (S) e um controle, sendo eles, S1: somente bagaço; S2: 75% bagaço e 25% serragem (3:1); S3: 50% bagaço e 50% serragem (1:1); S4: 25% bagaço e 75% serragem (1:3) e S5: somente serragem (controle). Os resultados mostraram que os bagaços de V. labrusca e M. domestica e serragem de Pinus sp. foram adequados à produção de cogumelos de P. sajor-caju quando utilizados isolados ou em combinações, destacando-se a combinação S4 com V. labrusca, onde foram obtidos os melhores resultados de produtividade (P), eficiência biológica (EB) e rendimento (R), de 0,59 g.dia-1, 33% e 11%, respectivamente. O substrato que possibilitou melhor atividade de lacases (Lac) foi S1 com M. domestica (81.995 U.g-1 no 30º dia), bem como a maior atividade de lignina peroxidase (LiP - 26 U.g-1) e oxidases do álcool veratrílico (OAV - 20,9 U.g-1). A maior concentração de proteínas totais (PT - 2.803 U.g-1) foi obtida em S4 com V. labrusca. Em S3, com bagaço de M. domestica, P. sajor-caju apresentou maior atividade de manganês peroxidase (MnP - 636,4 U.g-1) e a maior biomassa foi obtida em S3 com V. labrusca (8,47 mg de N-acetilglicosamina/g de substrato no 24º dia). Para L. edodes somente V. labrusca (S4) mostrou produção de cogumelos com R de 12,8%, EB de 38,5% e P de 0,25 g.dia-1. Já o bagaço de M. domestica possibilitou a produção de cogumelos nas combinações S3, S4 e controle S5. S4 com V. labrusca possibilitou a maior atividade de Lac por L. edodes (2.932 U.g-1 no 4º dia de cultivo) e a mais alta atividade de LiP (2.73 U g-1). Como a maior biomassa foi obtida empregando o substrato S1 com V. labrusca no 16º dia (17,42 mg de N-acetilglicosamina/g de substrato) e em S4 ocorreu a mais alta atividade de lacases, isto mostra que o crescimento fúngico não está associado ao aumento na produção de Lac. S1 com V. labrusca também apresentou maiores níveis de OAV (25,94 U.g-1). Mais alta concentração de proteínas solúveis PT (6.858 U.g-1) foi obtida empregando S1 com M. domestica. MnP e peroxidases totais de L. edodes não foram detectadas nos substratos V. labrusca ou M. domestica. O bagaço de V. labrusca demonstrou ser mais adequado à atividade de Lac por L. edodes do que M. domestica. / Submitted by Ana Guimarães Pereira (agpereir@ucs.br) on 2016-12-09T15:57:07Z No. of bitstreams: 1 Dissertacao Jane Maria Finimundi.pdf: 2306012 bytes, checksum: 9b748065880a8d067aa4ca4e2d374bd9 (MD5) / Made available in DSpace on 2016-12-09T15:57:07Z (GMT). No. of bitstreams: 1 Dissertacao Jane Maria Finimundi.pdf: 2306012 bytes, checksum: 9b748065880a8d067aa4ca4e2d374bd9 (MD5) Previous issue date: 2016-12-09 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES. / Edible mushrooms Pleurotus sajor-caju PS-2001 and Lentinula edodes LE-01 were cultivated on substrates constituted of sawdust of Pinus sp. (for P. sajor-caju) or Eucalyptus sp. ( for L. edodes) added of Vitis labrusca and Malus domestica bagasse residues. The production parameters evaluated were: productivity (P), biological efficiency (BE) and yield (Y)), activities of enzymes (laccases, VAO, MnP and LiP), total soluble proteins and the N-acetyl glucosamine liberated from mycelia as a indirect estimative of biomass growth. There were three combinations of substrates (S) and a control, S1: only bagasse residues, S2: 75% bagasse residues and 25% sawdust (3:1), S3: 50% bagasse residues and 50% sawdust (1 : 1), S4: 25% bagasse residues and 75% sawdust (1:3) and S5: sawdust only (control). The results showed that bagasse residues of V. labrusca and M. domestica and sawdust of Pinus sp. were suitable for mushroom production of P. sajor-caju when used alone or in combinations, especially S4 with V. labrusca, which obtained the best results in productivity (P), biological efficiency (BE) and yield (Y) of 0.59 g day-1, 33% and 11% respectively. The substrate which showed the highest laccase activity (Lac) was S1 with M. domestica (81,995 U g-1) and the highest activity of lignin peroxidase (LiP - 26 U g-1) and also veratryl alcohol oxidase (VAO - 20.9 U g-1). The highest concentration of total protein (TP - 2803 mg g-1 of dry matter) was obtained in S4 with V. labrusca. In S3, with bagasse residues of M. domestica, P. sajor-caju showed higher activity of manganese peroxidase (MnP - 636.4 U g-1). The highest biomass was obtained in S3 with V. labrusca (8.47 mg N-acetyl glucosamine/g substrate on the 24 th day). To L. edodes, only V. labrusca ( S4) showed mushrooms production with a R of 12.8%, EB of 38.5% and Y of 0.25 g day-1. Only the bagasse M. domestica enabled the production of mushrooms in S3, S4 and S5. S4 with V. labrusca also showed the highest activity of Lac (2932 U g-1 on day 4 of culture) and the highest activity of LiP (2.73 U g-1). As the highest biomass was obtained in S1 with V. labrusca on the 16th day (17.42 mg N-acetyl glucosamine / g substrate) and S4 showed highest laccases secretion, this can show that fungal growth is not associated with increased production of Lac. S1 with V. labrusca also showed higher levels of VAO (25.94 U g-1). Higher concentration of TP (6858 mg g-1) was obtained from S1 with M. domestica. MnP and total peroxidases of L. edodes were not detected in V. labrusca or M. domestica. V. labrusca was more appropriate for laccase activity of L. edodes than M. domestica.
23

Bioatividades de frações dos extratos de Lentinula edodes e Pleurotus sajor-caju em cultivo de células de mamíferos

Finimundy, Tiane Cristine 08 December 2017 (has links)
Os cogumelos têm vastas perspectivas como fontes de propriedades medicinais, pois são usados desde os tempos remotos para tratar doenças e promover a saúde em geral. Possuem uma quantidade de compostos bioativos interessantes o que despertou o interesse da área científica. O presente estudo tem como objetivo a avaliação das propriedades bioativas e da composição química e nutricional dos cogumelos Pleurotus sajor-caju e Lentinula edodes. A atividade antioxidante do extrato foi avaliada através de ensaios de DPPH, poder redutor, TBARS e betacaroteno. Foram realizadas atividade antitumoral nas células NCI-H460, HepG2, HeLa, MCF-7 PLP2 HCT-116 e MRC-5, e anti-inflamatória para o extrato etanólico de P. sajor-caju em Raw 264.7. A composição da amostra do P. sajor caju em tocoferóis foi determinada por Cromatografia Líquida de Alta Eficiência (CLAE) acoplada a um detector de arranjo de diodos (DAD). Os perfis em ácidos graxos e açúcares foram obtidos por cromatografia a gas acoplada a um detector de ionização de chama (CG-FID) e por CLAE acoplado a um detector de índice de refração (DIR). O P. sajor-caju mostrou ser fonte importante de antioxidantes principalmente fenóis (147,54 μg/g) e tocoferóis (615.9g/100g). β-tocoferol foi a forma encontrada em maiores quantidades. Este extrato apresentou atividade antioxidante, sendo esta mais significativa para os ensaios de inibição da peroxidação lipídica (valores de EC50 inferiores a 2,6 mg/mL). A determinação do perfil em macronutrientes demonstrou altos teores em proteínas (17,29 g/100 g) e carboidratos (76,18 g/100 g), apresentando baixos valores de lipídios (1,16 g/100 g). A análise da composição do ácidos graxos conduziu à quantificação de 22 moléculas, com predomínio dos ácidos graxos poli-insaturados, particularmente os ácidos oleico e linoleico, 17-61% e 20-54%, respectivamente. O ensaio de citometria de fluxo foi utilizado para a verificação do tipo de morte induzida, para ambos os cogumelos, que apresentaram resultados semelhantes, indicando morte por apoptose. Os estudos efetuados descrevem o potencial nutracêutico das espécies analisadas, bem como o docking molecular nos faz pensar em uma possível atividade pró-apoptótica e anti-inflamatória. O consumo de cogumelos in natura como em extratos podem exercer um efeito benéfico para a saúde e auxiliar no tratamento de determinadas doenças. / Submitted by cmquadros@ucs.br (cmquadros@ucs.br) on 2018-04-03T14:30:36Z No. of bitstreams: 1 Tese Tiane C Finimundy.pdf: 5212206 bytes, checksum: ca4d07dbacc3899085628cc06e5f33d3 (MD5) / Made available in DSpace on 2018-04-03T14:30:36Z (GMT). No. of bitstreams: 1 Tese Tiane C Finimundy.pdf: 5212206 bytes, checksum: ca4d07dbacc3899085628cc06e5f33d3 (MD5) Previous issue date: 2018-04-03 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES / Mushrooms have vast prospects as sources of medicinal properties, since they have been used since ancient times to treat diseases and promote health in general. They have a number of interesting bioactive compounds which aroused the interest of the scientific area. The present study aims to evaluate the bioactive properties and the chemical and nutritional composition of the Pleurotus sajor-caju and Lentinula edodes mushrooms. The antioxidant activity of the extract was evaluated through DPPH, reducing power, TBARS and beta carotene assays. Antitumor activity was performed on NCI-H460, HepG2, HeLa, MCF-7, PLP2, HCT-116 and MRC-5 cells, and anti-inflammatory for the ethanolic extract of P. sajor-caju on Raw 264.7. The composition of the P. sajor caju sample in tocopherols was determined by High Performance Liquid Chromatography (HPLC) coupled to a fluorescence detector (DAD). The fatty acid and sugar profiles were obtained by gas chromatography coupled to a flame ionization detector (GC-FID) and by HPLC coupled to a refractive index detector (RID). P. sajor-caju showed to be an important source of antioxidants mainly phenols (147.54 μg / g) and tocopherols (615.9g / 100g). β-tocopherol was the form found in higher amounts. This extract showed antioxidant activity, which is more significant for lipid peroxidation inhibition assays (EC 50 values below 2.6 mg / mL). Macronutrient profile determination showed high levels of protein (17.29 g / 100 g) and carbohydrates (76.18 g / 100 g), presenting low lipid values (1.16 g / 100 g). Analysis of the composition of fatty acids led to the quantification of 22 molecules, with predominance of polyunsaturated fatty acids, particularly oleic and linoleic acids, 17-61% and 20-54%, respectively. The flow cytometry assay was used to verify the type of induced death for both mushrooms, which showed similar results, indicating death by apoptosis. The studies carried out describe the nutraceutical potential of the analyzed species, as well as the molecular docking makes us think of a possible pro-apoptotic and anti-inflammatory activity. The consumption of mushrooms in nature as in extracts can have a beneficial effect on health and help in the treatment of certain diseases.
24

Bioatividades de frações dos extratos de Lentinula edodes e Pleurotus sajor-caju em cultivo de células de mamíferos

Finimundy, Tiane Cristine 08 December 2017 (has links)
Os cogumelos têm vastas perspectivas como fontes de propriedades medicinais, pois são usados desde os tempos remotos para tratar doenças e promover a saúde em geral. Possuem uma quantidade de compostos bioativos interessantes o que despertou o interesse da área científica. O presente estudo tem como objetivo a avaliação das propriedades bioativas e da composição química e nutricional dos cogumelos Pleurotus sajor-caju e Lentinula edodes. A atividade antioxidante do extrato foi avaliada através de ensaios de DPPH, poder redutor, TBARS e betacaroteno. Foram realizadas atividade antitumoral nas células NCI-H460, HepG2, HeLa, MCF-7 PLP2 HCT-116 e MRC-5, e anti-inflamatória para o extrato etanólico de P. sajor-caju em Raw 264.7. A composição da amostra do P. sajor caju em tocoferóis foi determinada por Cromatografia Líquida de Alta Eficiência (CLAE) acoplada a um detector de arranjo de diodos (DAD). Os perfis em ácidos graxos e açúcares foram obtidos por cromatografia a gas acoplada a um detector de ionização de chama (CG-FID) e por CLAE acoplado a um detector de índice de refração (DIR). O P. sajor-caju mostrou ser fonte importante de antioxidantes principalmente fenóis (147,54 μg/g) e tocoferóis (615.9g/100g). β-tocoferol foi a forma encontrada em maiores quantidades. Este extrato apresentou atividade antioxidante, sendo esta mais significativa para os ensaios de inibição da peroxidação lipídica (valores de EC50 inferiores a 2,6 mg/mL). A determinação do perfil em macronutrientes demonstrou altos teores em proteínas (17,29 g/100 g) e carboidratos (76,18 g/100 g), apresentando baixos valores de lipídios (1,16 g/100 g). A análise da composição do ácidos graxos conduziu à quantificação de 22 moléculas, com predomínio dos ácidos graxos poli-insaturados, particularmente os ácidos oleico e linoleico, 17-61% e 20-54%, respectivamente. O ensaio de citometria de fluxo foi utilizado para a verificação do tipo de morte induzida, para ambos os cogumelos, que apresentaram resultados semelhantes, indicando morte por apoptose. Os estudos efetuados descrevem o potencial nutracêutico das espécies analisadas, bem como o docking molecular nos faz pensar em uma possível atividade pró-apoptótica e anti-inflamatória. O consumo de cogumelos in natura como em extratos podem exercer um efeito benéfico para a saúde e auxiliar no tratamento de determinadas doenças. / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES / Mushrooms have vast prospects as sources of medicinal properties, since they have been used since ancient times to treat diseases and promote health in general. They have a number of interesting bioactive compounds which aroused the interest of the scientific area. The present study aims to evaluate the bioactive properties and the chemical and nutritional composition of the Pleurotus sajor-caju and Lentinula edodes mushrooms. The antioxidant activity of the extract was evaluated through DPPH, reducing power, TBARS and beta carotene assays. Antitumor activity was performed on NCI-H460, HepG2, HeLa, MCF-7, PLP2, HCT-116 and MRC-5 cells, and anti-inflammatory for the ethanolic extract of P. sajor-caju on Raw 264.7. The composition of the P. sajor caju sample in tocopherols was determined by High Performance Liquid Chromatography (HPLC) coupled to a fluorescence detector (DAD). The fatty acid and sugar profiles were obtained by gas chromatography coupled to a flame ionization detector (GC-FID) and by HPLC coupled to a refractive index detector (RID). P. sajor-caju showed to be an important source of antioxidants mainly phenols (147.54 μg / g) and tocopherols (615.9g / 100g). β-tocopherol was the form found in higher amounts. This extract showed antioxidant activity, which is more significant for lipid peroxidation inhibition assays (EC 50 values below 2.6 mg / mL). Macronutrient profile determination showed high levels of protein (17.29 g / 100 g) and carbohydrates (76.18 g / 100 g), presenting low lipid values (1.16 g / 100 g). Analysis of the composition of fatty acids led to the quantification of 22 molecules, with predominance of polyunsaturated fatty acids, particularly oleic and linoleic acids, 17-61% and 20-54%, respectively. The flow cytometry assay was used to verify the type of induced death for both mushrooms, which showed similar results, indicating death by apoptosis. The studies carried out describe the nutraceutical potential of the analyzed species, as well as the molecular docking makes us think of a possible pro-apoptotic and anti-inflammatory activity. The consumption of mushrooms in nature as in extracts can have a beneficial effect on health and help in the treatment of certain diseases.
25

Produção de cogumelos comestíveis e fenol-oxidases de Pleurotus sajor-caju e Lentinula edodes em resíduos lignocelulósicos

Finimundi, Jane Maria 11 November 2011 (has links)
Cogumelos comestíveis de Pleurotus sajor-caju PS-2001 e Lentinula edodes LE-01 foram cultivados em substratos constituídos de serragem de Pinus sp ( para P. sajor-caju) ou Eucalyptus sp (para L. edodes) adicionados de resíduos de bagaços de Vitis labrusca e Malus domestica. Foram avaliados os parâmetros de produção produtividade (P), eficiência biológica e rendimento (R), atividade das enzimas (lacases, VAO, MnP and LiP), proteína solúvel total e a N-acetil glicosamina liberada do micélio como uma estimativa indireta do crescimento da biomassa. Os testes foram realizados com os bagaços dos dois frutos separadamente e em serragem de Pinus sp. (para P. sajor-caju) e Eucalyptus sp.. Foram utilizadas três combinações de substratos (S) e um controle, sendo eles, S1: somente bagaço; S2: 75% bagaço e 25% serragem (3:1); S3: 50% bagaço e 50% serragem (1:1); S4: 25% bagaço e 75% serragem (1:3) e S5: somente serragem (controle). Os resultados mostraram que os bagaços de V. labrusca e M. domestica e serragem de Pinus sp. foram adequados à produção de cogumelos de P. sajor-caju quando utilizados isolados ou em combinações, destacando-se a combinação S4 com V. labrusca, onde foram obtidos os melhores resultados de produtividade (P), eficiência biológica (EB) e rendimento (R), de 0,59 g.dia-1, 33% e 11%, respectivamente. O substrato que possibilitou melhor atividade de lacases (Lac) foi S1 com M. domestica (81.995 U.g-1 no 30º dia), bem como a maior atividade de lignina peroxidase (LiP - 26 U.g-1) e oxidases do álcool veratrílico (OAV - 20,9 U.g-1). A maior concentração de proteínas totais (PT - 2.803 U.g-1) foi obtida em S4 com V. labrusca. Em S3, com bagaço de M. domestica, P. sajor-caju apresentou maior atividade de manganês peroxidase (MnP - 636,4 U.g-1) e a maior biomassa foi obtida em S3 com V. labrusca (8,47 mg de N-acetilglicosamina/g de substrato no 24º dia). Para L. edodes somente V. labrusca (S4) mostrou produção de cogumelos com R de 12,8%, EB de 38,5% e P de 0,25 g.dia-1. Já o bagaço de M. domestica possibilitou a produção de cogumelos nas combinações S3, S4 e controle S5. S4 com V. labrusca possibilitou a maior atividade de Lac por L. edodes (2.932 U.g-1 no 4º dia de cultivo) e a mais alta atividade de LiP (2.73 U g-1). Como a maior biomassa foi obtida empregando o substrato S1 com V. labrusca no 16º dia (17,42 mg de N-acetilglicosamina/g de substrato) e em S4 ocorreu a mais alta atividade de lacases, isto mostra que o crescimento fúngico não está associado ao aumento na produção de Lac. S1 com V. labrusca também apresentou maiores níveis de OAV (25,94 U.g-1). Mais alta concentração de proteínas solúveis PT (6.858 U.g-1) foi obtida empregando S1 com M. domestica. MnP e peroxidases totais de L. edodes não foram detectadas nos substratos V. labrusca ou M. domestica. O bagaço de V. labrusca demonstrou ser mais adequado à atividade de Lac por L. edodes do que M. domestica. / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES. / Edible mushrooms Pleurotus sajor-caju PS-2001 and Lentinula edodes LE-01 were cultivated on substrates constituted of sawdust of Pinus sp. (for P. sajor-caju) or Eucalyptus sp. ( for L. edodes) added of Vitis labrusca and Malus domestica bagasse residues. The production parameters evaluated were: productivity (P), biological efficiency (BE) and yield (Y)), activities of enzymes (laccases, VAO, MnP and LiP), total soluble proteins and the N-acetyl glucosamine liberated from mycelia as a indirect estimative of biomass growth. There were three combinations of substrates (S) and a control, S1: only bagasse residues, S2: 75% bagasse residues and 25% sawdust (3:1), S3: 50% bagasse residues and 50% sawdust (1 : 1), S4: 25% bagasse residues and 75% sawdust (1:3) and S5: sawdust only (control). The results showed that bagasse residues of V. labrusca and M. domestica and sawdust of Pinus sp. were suitable for mushroom production of P. sajor-caju when used alone or in combinations, especially S4 with V. labrusca, which obtained the best results in productivity (P), biological efficiency (BE) and yield (Y) of 0.59 g day-1, 33% and 11% respectively. The substrate which showed the highest laccase activity (Lac) was S1 with M. domestica (81,995 U g-1) and the highest activity of lignin peroxidase (LiP - 26 U g-1) and also veratryl alcohol oxidase (VAO - 20.9 U g-1). The highest concentration of total protein (TP - 2803 mg g-1 of dry matter) was obtained in S4 with V. labrusca. In S3, with bagasse residues of M. domestica, P. sajor-caju showed higher activity of manganese peroxidase (MnP - 636.4 U g-1). The highest biomass was obtained in S3 with V. labrusca (8.47 mg N-acetyl glucosamine/g substrate on the 24 th day). To L. edodes, only V. labrusca ( S4) showed mushrooms production with a R of 12.8%, EB of 38.5% and Y of 0.25 g day-1. Only the bagasse M. domestica enabled the production of mushrooms in S3, S4 and S5. S4 with V. labrusca also showed the highest activity of Lac (2932 U g-1 on day 4 of culture) and the highest activity of LiP (2.73 U g-1). As the highest biomass was obtained in S1 with V. labrusca on the 16th day (17.42 mg N-acetyl glucosamine / g substrate) and S4 showed highest laccases secretion, this can show that fungal growth is not associated with increased production of Lac. S1 with V. labrusca also showed higher levels of VAO (25.94 U g-1). Higher concentration of TP (6858 mg g-1) was obtained from S1 with M. domestica. MnP and total peroxidases of L. edodes were not detected in V. labrusca or M. domestica. V. labrusca was more appropriate for laccase activity of L. edodes than M. domestica.
26

Control of phenotypic variation in Pseudomonas tolaasii

Smith, Julia C. January 1996 (has links)
No description available.
27

Makromycety okolí Řevnic / Macromycetes of Řevnice neighborhood

Marhoulová, Eliška January 2013 (has links)
This thesis is a summary of basic information about macromycetes, their particular way of life and identification cognitive characteristics. It also includes processing research areas Hřebeny - Brdy between Dobřichovice and Řevnice. The research results are applied in primary school instruction. As a part of this work is the proposal excursion to this area and trail design. Key words: macromycetes, mushrooms, Brdy
28

The effects of calcium and manganese on edible mushroom pleurotus pulmonarius.

January 1997 (has links)
by Law Shui Chee Annie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 117-125). / Abstract --- p.i / Abbreviations --- p.iii / List of Figures --- p.iv / List of Tables --- p.vi / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- Background of Pleurotus pulmonarius --- p.1 / Chapter 1.1.1 --- Taxonomy and classification --- p.1 / Chapter 1.1.2 --- Life cycle --- p.2 / Chapter 1.1.3 --- Marketing value --- p.4 / Chapter 1.1.4 --- Nutritional content --- p.4 / Chapter 1.2 --- Background of calcium --- p.5 / Chapter 1.2.1 --- Calcium requirements for human --- p.6 / Chapter 1.2.2 --- The biological role of calcium --- p.6 / Chapter 1.2.3 --- Calcium as a regulator --- p.8 / Chapter 1.2.4 --- Binding of calcium --- p.9 / Chapter 1.2.5 --- Uptake of calcium --- p.11 / Chapter 1.2.6 --- Calcium transport --- p.11 / Chapter 1.2.7 --- Application of calcium on mushroom cultivation --- p.12 / Chapter 1.2.8 --- Calcium effect on mushroom --- p.13 / Chapter 1.3 --- Background of manganese --- p.13 / Chapter 1.3.1 --- The biological role of manganese --- p.14 / Chapter 1.3.2 --- Uptake of manganese --- p.15 / Chapter 1.3.3 --- Manganese requirements of humans --- p.16 / Chapter 1.3.4 --- Manganese deficiency --- p.16 / Chapter 1.3.5 --- Use of manganese --- p.17 / Chapter 1.3.6 --- Influence of pH on manganese toxicity --- p.17 / Chapter 1.3.7 --- Effects of manganese on enzymes --- p.18 / Chapter 1.3.8 --- Application of manganese on mushroom cultivation --- p.19 / Chapter 1.4 --- Purpose of Study --- p.19 / Chapter 2. --- MATERIALS AND METHODS --- p.21 / Chapter 2.1 --- Organisms --- p.21 / Chapter 2.2 --- Maintenance of cultures --- p.21 / Chapter 2.3 --- Identification of two strains --- p.21 / Chapter 2.3.1 --- Determination of growth rate --- p.22 / Chapter 2.3.2 --- Arbitrarily-primed polymerase chain reaction (AP-PCR) --- p.22 / Chapter 2.3.3 --- Mating type reaction --- p.25 / Chapter 2.4 --- Effect of different concentrations of calcium and manganese on the life cycle of the fungi --- p.26 / Chapter 2.4.1 --- Spore germination --- p.26 / Chapter 2.4.2 --- Preparation of mycelium homogenate --- p.27 / Chapter 2.4.3 --- Vegetative growth --- p.28 / Chapter 2.4.4 --- Fruiting initiation --- p.30 / Chapter 2.4.5 --- Fruiting --- p.31 / Chapter 2.4.6 --- Fruiting yield (Biological efficiency) --- p.32 / Chapter 3. --- RESULTS --- p.35 / Chapter 3.1 --- Identification of two strains --- p.35 / Chapter 3.1.1 --- Determination of growth rate --- p.35 / Chapter 3.1.2 --- Fruitbody morphology --- p.35 / Chapter 3.1.3 --- Arbitrarily primed polymerase chain reaction (AP-PCR) --- p.35 / Chapter 3.1.4 --- Mating type reaction --- p.40 / Chapter 3.2 --- Effect of calcium and manganese on the life cycle of the fungus --- p.40 / Chapter 3.2.1 --- Spore germination --- p.40 / Chapter 3.2.2 --- Vegetative growth --- p.44 / Chapter 3.2.3 --- Fruiting initiation in vitro --- p.58 / Chapter 3.2.4 --- Fruiting --- p.58 / Chapter 4. --- DISCUSSION --- p.96 / Chapter 4.1 --- Effects of calcium and manganese on spore germination --- p.96 / Chapter 4.2 --- Effects of calcium and manganese on vegetative growth --- p.97 / Chapter 4.2.1 --- Biomass study --- p.97 / Chapter 4.2.2 --- Glucose utilization --- p.99 / Chapter 4.2.3 --- Protein secretion --- p.99 / Chapter 4.2.4 --- Orthophosphate utilization --- p.102 / Chapter 4.2.5 --- Ammonia content study --- p.104 / Chapter 4.2.6 --- Metal content study --- p.106 / Chapter 4.3 --- Effects of calcium and manganese on fruiting initiation --- p.107 / Chapter 4.4 --- Effects of calcium and manganese on fruiting --- p.107 / Chapter 4.4.1 --- Fruiting yield (biological efficiency) --- p.109 / Chapter 4.4.2 --- Metal content in fruitbodies --- p.109 / Chapter 4.4.3 --- "Carbon, hydrogen, nitrogen and sulfur contents in fruitbodies" --- p.111 / Chapter 4.4.3 --- Amino acid content in fruitbodies --- p.114 / Chapter 4.5 --- Response of different stages towards metals --- p.115 / Chapter 5. --- CONCLUSION --- p.116 / Chapter 6. --- REFERENCES --- p.117 / Chapter 7. --- APPENDIX --- p.126 / Chapter 7.1 --- Preparation of reagents for determination of orthophosphate content --- p.126 / Chapter 7.1.1 --- Stock standard 100.0 mg P/L --- p.126 / Chapter 7.1.2 --- Working stock standard solution 10.0 mg P/L --- p.126 / Chapter 7.1.3 --- Stock ammonium molybdate solution --- p.126 / Chapter 7.1.4 --- Stock antimony potassium tartrate solution --- p.126 / Chapter 7.1.5 --- Molybdate color reagent --- p.126 / Chapter 7.1.6 --- Ascorbic acid reducing solution --- p.127 / Chapter 7.1.7 --- Sodium hydroxide-EDTA rinse --- p.127 / Chapter 7.2 --- Preparation of reagents for determination of ammonia content --- p.127 / Chapter 7.2.1 --- Stock standard 100.0 mgN/L as NH3 in 2 M KC1 --- p.127 / Chapter 7.2.2 --- Working standards --- p.127 / Chapter 7.2.3 --- Potassium chloride and standards diluent --- p.127 / Chapter 7.2.4 --- EDTA solution --- p.127 / Chapter 7.2.5 --- Buffer --- p.127 / Chapter 7.2.6 --- Salicylate-nitroprusside color reagent recipe --- p.128 / Chapter 7.2.7 --- Hypochlorite reagent --- p.128
29

Controlling the forest understory : wild mushroom politics in central Oregon /

McLain, Rebecca J. January 2000 (has links)
Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 422-459).
30

Factors affecting the selectivity of composts suitable for the cultivation of Agaricus species

Smith, Jeffrey Francis January 1994 (has links)
No description available.

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