Zugl.: Aachen, Techn. Hochsch., Diss., 2009.

Zugl.: Wuppertal, Univ., Diss., 2007

This thesis consists of an investigation of the current use of visual representations, and drawing in particular, when teaching and assessing within anatomy education in medical studies. Although we know a lot about teachers’ use of visual representations and increasingly drawing, especially in the context of science education, less is known about the use of those tools by educators in the anatomical domain. Drawing is not currently systematically being used within assessment in anatomy education in UK medical schools, and its potential in assessment has not been investigated in depth. Four studies were conducted to answer the research questions set for this thesis. The first study investigated the way in which the teaching staff understand the use of visual representations in teaching, learning and assessment in anatomy. To answer this, observations of seven anatomy demonstrators were conducted within Year 1 dissection sessions, and interviews were conducted with the same demonstrators. The second study employed a real-world experimental design to explore if students’ drawings reveal changes in their understanding after dissection. Drawings from 98 Year 1 medical students were analysed in a within-subject crossover design, where students in the first condition drew the exterior of the heart before dissection in Week 1 and drew the superior mediastinum after dissection in Week 2. The order was reversed for students in the second condition. All drawings were analysed for their content and form with an extensive coding scheme that was developed for this thesis, as the existing coding approaches towards drawings were judged as inappropriate. The third study investigated the use of drawing to reveal changes in understanding as the medical degree progresses. Drawings of the exterior of the heart from 46 Year 3 students were analysed and compared to drawings from the Year 1 students. Finally, the fourth study investigated the way in which anatomy demonstrators understand drawing in the assessment of anatomical knowledge; eight artefact-based interviews were conducted with anatomy demonstrators. The findings offer important insights into the way in which visual representations and drawing in particular are being used by anatomy demonstrators within anatomy education and their perspectives on the use of drawing in teaching, learning and assessment. A significant contribution is also made to the knowledge regarding the use of drawing to assess understanding in spatially intensive domains, such as the anatomical one. This thesis also contributes to our knowledge of dissection as a method of teaching anatomy and the need for drawing training within this domain. Finally, the scheme for drawing analysis that was developed, evaluated and employed in this thesis can be considered a methodological contribution to the approaches of analysing drawings in the current literature.

Made available in DSpace on 2014-06-12T17:34:59Z (GMT). No. of bitstreams: 2 arquivo2004_1.pdf: 3200148 bytes, checksum: f3828ef94d92679f7e75b61c5527b68b (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2008 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / No âmbito deste trabalho foi abordada a problemática da geração automática do código NC para o processo de usinagem por fresamento em 2 ½ eixos. Nele foi desenvolvida uma metodologia que visa auxiliar a obtenção dos parâmetros necessários para a construção do programa de comando numérico (código NC). Esta metodologia propõe desenvolver um sistema de reconhecimento geométrico. O sistema lê arquivos neutros como entrada e traduz a informação em um arquivo de informação de fabricação. A metodologia se divide nas seguintes partes a considerar: 1) Extrações das informações geométricas do CAD; 2) Determinação das seqüências de corte; 3) Otimização dos parâmetros de usinagem e 4) Geração do código NC propriamente dito. A extração das informações geométricas é realizada através de um programa computacional desenvolvido neste trabalho, que captura as informações de dois arquivos neutros em formato IGES, um constituindo a peça bruta e o outro constituindo a peça ser fabricada. A informação geométrica de contorno (B-rep) é então analisada, onde através de processamento, os dados são armazenados em uma estrutura de grafo. Com a estrutura de grafo montada, podem-se obter informações, através das quais o programa fornece com relativa versatilidade as seqüências de operações de corte, que deverão ser executadas para a fabricação da peça. Após o reconhecimento geométrico e o sequenciamento das operações é realizada a otimização das condições de corte e dos percursos das ferramentas para cada operação, os quais são obtidos considerando-se o critério de mínimo tempo total de fabricação por peça, ou seja, critério de máxima produção. O programa computacional, assim, gera automaticamente programas em linguagem de máquina (código NC), para posterior envio a uma máquina CNC. O programa computacional foi desenvolvido por programação orientada a objetos (OOP) e escrito em linguagem C++ tendo como domínio o campo de peças prismáticas de base poligonal que possam ser usinadas em uma fresadora ou centro de usinagem CNC

Submitted by isabela.moljf@hotmail.com (isabela.moljf@hotmail.com) on 2017-07-05T15:18:16Z No. of bitstreams: 1 mateusviniciusmarcial.pdf: 2235520 bytes, checksum: 25f39d17f0416ae1fc55b0285fe52d56 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-08-08T17:46:01Z (GMT) No. of bitstreams: 1 mateusviniciusmarcial.pdf: 2235520 bytes, checksum: 25f39d17f0416ae1fc55b0285fe52d56 (MD5) / Made available in DSpace on 2017-08-08T17:46:01Z (GMT). No. of bitstreams: 1 mateusviniciusmarcial.pdf: 2235520 bytes, checksum: 25f39d17f0416ae1fc55b0285fe52d56 (MD5) Previous issue date: 2013-07-31 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Neste trabalho é estudado uma maneira de se introduzir não-comutatividade em alguns sistemas físicos via estruturas simpléticas para se investigar as propriedades de espaços não-comutativos (NC). Inicialmente, foi discutido em detalhes uma maneira sistemática de se introduzir não-comutatividade baseado no formalismo de Faddeev-Jackiw denominada Formalismo Simplético de Indução de Não-Comutatividade (FSINC). Este formalismo foi usado pra se obter uma versão NC para o Modelo Sigma Não-linear O(3) e para o Modelo de Skyrme SU(2). Posteriormente, uma segunda lei de Newton modificada que preserva a invariância rotacional foi obtida em um espaço de fase clássico estendido NC. Entre os principais efeitos da não-comutatividade na dinâmica de um oscilador harmônico tratado nesse espaço nota-se que a não-comutatividade induz uma pertubação estável no oscilador harmônico usual e que o oscilador pode até mesmo deixar de ser periódico dependendo da relação entre as frequências de oscilação da coordenada NC e do momento linear. Em seguida, considerando um espaço de fase com estrutura simplética não-comutativa e aplicando o (FSINC), as equações Friedmann- Lemaître corrigidas foram obtidas. As correções nas equações Friedmann- Lemaître podem ser associados com um fluido perfeito NC. Finalmente, usando as equações de Friedmann-Lemaître corrigidas, o parâmetro desaceleração NC pode ser determinado em termos do redshift. Dos valores existentes na literatura para o parâmetro densidade de energia do vácuo Ω0Ʌ e para o redshift transição, estima-se que a ordem de grandeza do parâmetro densidade de matéria do fluido NC Ω0β e do parâmetro NC βsão iguais a 0,52+0,03 -0,159 e β= -0,784 +2,398 -0,453 x 10 -36s-2, respectivamente. Isso mostra que a não-comutatividade poderia ser responsável por até 8,2% da densidade de matéria do universo ou por um terço da matéria escura sem violar os valores na literatura para o redshift de transição. / In this work we have studied how to introduce noncommutativity to some physical systems through the symplectic structures to investigate the properties of the noncommutative (NC) spaces. Initially, we discussed in details one systematic way to introduce noncommutativity, based on Faddeev-Jackiw formalism, called symplectic formalism for induction of noncommutativity (SFINC). This formalism was used to obtain NC versions of the SU(2) Skyrme model and O(3) nonlinear sigma model. After that, the rotational invariant noncommutative Newton’s second law was written in the NC extended classical phase space. Among the main effects of noncommutativity in the dynamics of a harmonic oscillator treated in this space, we note that the noncommutativity induces a stable perturbation in the usual harmonic oscillator and the oscillator may even not be periodic depending on the ratio between the oscillation frequency of the position coordinate and the oscillation frequency of the NC coordinate. Subsequently, considering a phase space with NC symplectic structure and applying the SFINC we obtained the modified Friedmann-Lemaître equations, which have NC corrections. This correction can be interpreted as a NC perfect fluid, which would behave like dust during a period of radiation, preserving the law of conservation of energy. Finally, using the Friedmann-Lemaître equations the NC deceleration parameter q can be determined in terms of the redshift. From the values in literature for the vacuum-energy density parameter Ω0Ʌ and the transition redshift, the range of acceptable values of the matter density parameter of NC fluid Ω0β and the NC parameter β estimated are 0,052+0,03 −0,159 and β = −0,784+2,398−0,453 ×10−36s−2, respectively. This result shows that the NC corrections could be responsible for up to 8.2% of the matter density of the universe, or a third of the dark matter, without violating the literature values of the transition redshift.

This thesis deals with a creation of a control system of 3-axis milling machine. The beginning of the thesis is dedicated to the general description of the problematic of the numerically controlled machines and the technologies used in this branch. The next chapter describes mechanical and electronical adjustments of the machine. Following chapters are dedicated to the creation of PLC program and HMI using the TwinCAT software. In conclusion, this thesis deals with commissioning of the machine, measuring its geometrical accuracy and testing.

The advancement of Nucleic acids (DNA and RNA) sequencing technology has enabled many projects targeted towards the identification of genome structure and transcriptome complexity of organisms. The first conclusions of the human and mouse projects have underscored two important, yet unexpected, findings. First, while almost the entire genome is transcribed, only 5% of it encodes for proteins. Thereby, most transcripts are noncoding RNA. This includes both short RNA (<200 nucleotides (nt)) comprising piRNAs; microRNAs (miRNAs); endogenous Short Interfering RNAs (siRNAs) among others, and includes lncRNA (>200nt). Second, a significant portion of the mammalian genome (45%) is composed of Repeat Elements (REs). RE are mostly relics of ancestral viruses that during evolution have invaded the host genome by producing thousands of copies. Their roles within their host genomes have yet to be fully explored considering that they sometimes produce lncRNA, and have been shown to influence expression at the transcriptional and post-transcriptional levels. Moreover, because some REs can still mobilize within host genomes, host genomes have evolved mechanisms, mainly epigenetic, to maintain REs under tight control. Recent reports indicate that REs activity is regulated in somatic cells, particularily in the brain, suggesting a physiological role of RE mobilization during normal development. In this thesis, I focus on the analysis of ncRNAs, specifically REs; piRNAs; lncRNAs in human and mouse post-mitotic somatic cells. The main aspects of this analysis are: Using sRNA-Seq, I show that piRNAs, a class of ncRNAs responsible for the silencing of Transposable elements (TEs) in testes, are present also in adult mouse brain. Furthermore, their regulation shows only a subset of testes piRNAs are expressed in the brain and may be controlled by known neurogenesis factors. To investigate the dynamics of the transcriptome during cellular differentiation, I examined deep RNA-Seq and Cap Analysis of Gene Expression (CAGE) data from time-course progression program of primary human skeletal muscle cell differentiation. I contrasted this program with Duchenne Muscular Dystrophy (DMD) donors. I identified novel candidates, protein-coding genes and lncRNAs, that may be involved in myogenesis and reaffirmed known myogenic players. Using RNA-Seq data, I designed a novel pipeline to identify possible de novo insertion sites during muscular differentiation, which I have also tested on embryonic mouse cerebral cortex.

Ces dernières années sont apparues de nouvelles techniques permettant le contrôle de la charge de nano-objets individuels (atome, molécule, agrégat métallique ou semi-conducteur, ...) déposés sur substrats isolants. Cet aboutissement a été rendu possible par le perfectionnement des méthodes de microscopie à effet tunnel (STM) et à force atomique (AFM). En combinant ces outils, les précurseurs ont réussi à maîtriser l'état de charge d'un atome d'or déposé sur une bicouche de NaCl(001) sur substrat Cu(111). Par la suite, ce type de manipulation a été étendu à des systèmes moléculaires notamment au CEMES avec Cu(dbm)2. Ce sujet s'inscrit dans la continuité de ces études. L'objectif était d'analyser l'impact de l'augmentation de l'épaisseur du film isolant sur les mécanismes de charge. Cette problématique requière une quantification de l'état de charge du système ainsi qu'une mesure de l'épaisseur d'isolant. Dans ce travail, nous avons pu étudier des films de KBr et NaCl déposés sur des surfaces de Cu(111) et Ag(111). Pour ces études, que ce soit en courant tunnel (STM) ou en gradient de force (NC-AFM), le contrôle de l'état de pointe est essentiel. Lorsque l'on travaille sur substrat isolant, la pointe a tendance à collecter des contaminants qui en changent les propriétés électroniques. Or, pour charger un système de manière reproductible, il nous faut impérativement contrôler la métallicité de l'apex. Cette maîtrise passe par une re-préparation fréquente de la pointe sur une surface métallique, difficile à trouver dans le cas d'un film épais. Pour pallier à cette rareté, nous avons mis en place un masque de dépôt permettant un contrôle du gradient de l'épaisseur du film isolant tout en préservant des zones de métal libre. Cela nous a permis de réaliser nos mesures avec un état de pointe mieux contrôlé. L'instabilité de l'état de pointe nous a également conduit à effectuer des spectroscopies à courant régulé de type Z(V). En contrôlant ce courant, il est alors possible de minimiser l'interaction entre la pointe et le film isolant, préservant ainsi plus longtemps la pointe. Ces spectroscopies Z(V) permettent également d'augmenter la tension de mesure jusqu'à atteindre le régime d'émission de champ. Nous avons observé par cette méthode une variation de la modulation de l'amplitude des résonances d'émission de champ (FER) en fonction de l'épaisseur du film isolant. Une modélisation numérique par différences finies a été développée afin de comprendre ce phénomène. [..] / In recent years, new techniques have emerged to control the charge of individual nano-objects (atom, molecule, metal aggregate or semiconductor, etc.) deposited on insulating substrates. This achievement has been made possible by the refinement of Tunneling Microscopy (STM) and Atomic Force (AFM) methods. By combining these tools, the precursors succeeded in controlling the state of charge of a gold atom deposited on a NaCl (001) bilayer on a Cu (111) substrate. Subsequently, this type of manipulation has been extended to molecular systems, in particular at the CEMES with Cu(dbm)2. This subject is part of the continuity of these studies. The objective was to analyze the impact of the increase of the thickness of the insulating film on the charge mechanisms. This problem requires a quantification of the state of the system charge as well as a measurement of the insulation thickness. In this work, we have been able to study KBr and NaCl films deposited on Cu(111) and Ag(111) surfaces. For these studies, whether in tunnel current (STM) or force gradient (NC-AFM), the control of the tip state is essential. When working on an insulating substrate, the tip tends to collect contaminants that change their electronic properties. However, to charge a system in a reproducible way, we must imperatively control the metallicity of the apex. This control requires a frequent re-preparation of the tip on a metal surface, difficult to find in the case of a thick film. To overcome this scarcity, we have implemented a deposition mask allowing a control of the gradient of the thickness of the insulating film while preserving clean metal zones. This allowed us to carry out our measurements with a better controlled state of the tip. The instability of the tip state has also led us to perform Z (V) regulated current spectroscopies. By controlling this current, it is then possible to minimize the interaction between the tip and the insulating film, thus making the tip last longer. These Z (V) spectroscopies also make it possible to increase the measurement voltage until reaching the field emission regime. We have observed a variation of the modulation of the field emission resonances (FER) amplitude as a function of the thickness of the insulating film. [...]

Embora o melanoma represente apenas 4% das neoplasias malignas da pele, é considerado a mais grave por ser altamente etal. Em virtude da via MAPK (Mitogen activated protein kinase) estar intimamente ligada ao descontrole da proliferação celular, especialmente em melanoma, esta via se tornou um alvo para o desenvolvimento de terapias direcionadas a oncogenes, como os potentes quimioterápicos Vemurafenibe (inibidor da mutação V600E em BRAF - BRAFV600E) e Trametinibe (inibidor de MEK). Cada vez mais, melhores taxas de respostas vêm sendo alcançadas com os novos medicamentos, porém a maioria dos pacientes está sujeita a recidivas após 7 meses de tratamento devido ao desenvolvimento de quimioresistência, justificando a constante busca por novos compostos terapêuticos. Dados de nosso laboratório indicam que 4-nerolidilcatecol (4-NC) induz aumento na expressão de p53, produção de ROS e dano ao DNA, culminando em apoptose dependente de caspase-3 em células de melanoma por ser um inibidor proteassomal. Além disto, o 4-nerolidilcatecol (4- NC) demonstrou efeito inibitório na proliferação de células de melanoma em modelo de cultura organotípica de pele. Desta forma, este projeto visa avaliar a possibilidade de superação da quimioresistência aos inibidores de BRAF e de MEK, utilizando terapias combinatórias com 4-NC em células de melanoma humano resistentes a estes inibidores. Primeiramente, linhagens celulares de melanoma resistentes aos inibidores de BRAF (R) e BRAF/MEK (DR) foram geradas a partir de células parentais BRAF mutadas (P) e caracterizadas por MTT, microscopia de fluorescência e western blotting. Estas células foram submetidas ao tratamento com 4-NC que apresentou citotoxicidade na concentração de 30&#181;M, inibição de formação de colônias e diminuição na invasão em modelos in vitro de culturas 2D e 3D em todas as linhagens estudadas (P, R e DR). O 4-NC foi ainda capaz de induzir estresse de retículo endoplasmático com indução de apoptose. Visando a explorar o efeito terapêutico in vivo do 4-NC, outro estudo foi conduzido em animais submetidos a enxerto xenográfico com células parentais de melanoma NRAS mutadas. Após desenvolvimento do tumor, os animais foram tratados 3 vezes por semana durante 3 semanas com 4-NC (10 mg/kg) por via i.p. O 4-NC foi capaz de inibir em até 4 vezes o crescimento dos tumores xenográficos (4/10) quando comparado com os controles, com remissão completa do tumor em um animal. A expressão de p53 e PARP clivada foi aumentada nos tumores dos animais tratados, sugerindo apoptose. A expressão gênica de MMP2 e MMP14 estava diminuída nas mesmas amostras, demonstrando o papel do 4-NC na inibição da invasão do melanoma in vivo. Finalmente, a toxicidade sistêmica do 4-NC foi avaliada nas mesmas doses empregadas no ensaio in vivo de tumorigênese. A baixa toxicidade observada nos ensaios toxicológicos com tratamentos sub-crônicos com 4-NC e a citotoxicidade demonstrada em modelos xenográficos nos leva a considerar este composto como promissor para estudos futuros e sua aplicação no tratamento do melanoma cutâneo humano, incluindo pacientes resistentes aos inibidores de BRAF e MEK. / Melanoma accounts for only 4% of malignant neoplasms of the skin, but is considered the most serious because it is highly deadly. Because the MAPK (Mitogen activated protein kinase) pathway is closely linked to the lack of control of cell proliferation, especially in melanoma, this pathway has become a target for the development of oncogene-targeted therapies, such as the potent chemotherapeutic agents Vemurafenib (V600E mutation inhibitor in BRAF - BRAFV600E) and Trametinib (MEK inhibitor). Increasingly, better response rates have been achieved with the new drugs, but most patients are subject to relapses after 7 months of treatment due to several mechanisms, which justify the constant search for new therapeutic compounds. Data from our laboratory indicate that 4-nerolidylcatechol (4-NC) induces increased p53 expression, ROS production and DNA damage, culminating in caspase-3 dependent apoptosis in melanoma cells. The 4-NC compound demonstrated an inhibitory effect on melanoma cell proliferation in an organotypic skin culture model. Thus, this project aims to evaluate the possibility of overcoming the existing chemoresistance to BRAF and MEK inhibitors, using 4-NC combinatory therapies in human melanoma cells resistant to these inhibitors. Firstly, melanoma cell lines resistant to BRAF (R) and BRAF / MEK (DR) inhibitors were generated from naive cells mutated BRAF (N) and characterized by MTT, fluorescence microscopy and western blotting. These cells were submitted to 4-NC treatment that showed cytotoxicity with 30 &#181;M, inhibition of colony formation and decrease in invasion in 2D and 3D in vitro models in all cell line studied (N, R and DR). Furthermore, 4-NC was able to induce endoplasmic reticulum stress with apoptosis induction. In order to explore the in vivo therapeutic effect of 4-NC, an additional study was conducted using xenograft model with NRAS-mutated melanoma cell line. After tumor development, the animals were treated 3 times per week for 3 weeks with 4-NC (10 mg / kg) by i.p. injection. 4-NC was able to inhibit up to 4- fold the growth of xenograft tumors (4/10) when compared to controls, with complete tumor remission in one animal. Cleaved PARP and p53 expression were increased in the tumors of treated animals, suggesting apoptosis. MMP2 and MMP14 gene expression were decreased in the same samples, demonstrating the role of 4-NC in inhibiting melanoma invasion in vivo. Finally, the systemic toxicity of 4-NC was evaluated at the same doses employed in the in vivo tumorigenesis assay. The low toxicity observed in the toxicological assays with sub-chronic 4- NC treatments and the demonstrated cytotoxicity in xenograft models leads us to consider this compound as promising for future studies and its application in the treatment of cutaneous human melanoma, including patients resistants to BRAF and MEK inhibitors.

Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-08-30T13:40:29Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tese_gizele_lima_de_sa.pdf: 1115362 bytes, checksum: 4fe5eecfaee189766be87ff59c7e2a47 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-09-01T19:13:07Z (GMT) No. of bitstreams: 2 tese_gizele_lima_de_sa.pdf: 1115362 bytes, checksum: 4fe5eecfaee189766be87ff59c7e2a47 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-09-01T19:13:16Z (GMT) No. of bitstreams: 2 tese_gizele_lima_de_sa.pdf: 1115362 bytes, checksum: 4fe5eecfaee189766be87ff59c7e2a47 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-09-01T19:13:24Z (GMT). No. of bitstreams: 2 tese_gizele_lima_de_sa.pdf: 1115362 bytes, checksum: 4fe5eecfaee189766be87ff59c7e2a47 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-03-16 / Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul - FAPERGS / O Neospora caninum é um protozoário filogenéticamente relacionado a vários coccídeos de importância médico veterinária, além de ser um parasito intracelular obrigatório capaz de causar abortamentos em bovinos e paralisia neoromuscular em cães. A interação ligantes/receptores são requisitos que permitem que o parasito explore sua capacidade invasiva, sendo o contato inicial mediado por proteínas de superfície imunodominantes como Nc-p43 e Nc-p29. O estudo de antígenos e proteínas recombinantes de N. caninum gerou uma série de informações para aprimorar o diagnóstico e diferenciação deste protozoário e demais agentes a ele relacionados, principalmente com relação ao Toxoplasma gondii. No presente trabalho foi realizada uma revisão bibliográfica das proteínas envolvidas na interação hospedeiro-parasito utilizadas como alvos vacinais e na aplicação em testes diagnósticos devido a sua especificidade. Dentre as proteínas descritas, os antígenos de superfície Nc-p43 e Nc-p29 são amplamente estudados devido a sua especificidade e capacidade de induzer uma resposta imune protetiva, além de não apresentar reação cruzada com outros parasitos Apicomplexas quando utilizadas em ensaios diagnósticos. Frente a isso, propomos a expressão das proteínas específicas de N. caninum, Nc-p43 e Nc-p29 em sistema procarioto, para avaliar sua antigenicidade frente a soros de animais naturalmente infectados por N. caninum e especificidade quando testados frente a soros de animais infectados por T. gondii. A proteína rNc-p43 foi utilizada na produção de um anticorpo policlonal, que foi purificado, conjugado a peroxidase (HPR) e isotiocianato de fluoresceína (FITC) afim de detectar as proteínas recombinantes e nativas Nc-p43, espectivamente. pAb e pAb/HRP foram capazes de reconhecer rNcp-43, enquanto pAb/FITC se mostrou eficiente na imunomarcação do complexo apical de taquizoítos. Um ensaio imunoenzimático de bloqueio (b-ELISA) foi realizado para avaliar a performance do pAb/HRP como ferramenta de diagnóstico. A porcentagem de inibição média para as amostras de soros positivos e negativos de bovinos com neosporose obteve diferença estatística (P <0,0001). Estes resultados sugerem que o pAb pode ligar-se aos mesmos epítopos da Ncp-43 que os anticorpos anti-N. caninum de amostras positivas testadas. O b-ELISA utilizando o pAb/HRP representa uma opção interessante aos testes de diagnóstico disponíveis para a neosporose, uma vez que menos passos estão envolvidos na sua realização e seu formato evita a reatividade cruzada com anticorpos anti-espécie específicos. Em resumo, este trabalho descreve a clonagem e expressão das proteínas Nc-p43 e Nc-p29 de N. caninum em sistema procarioto, a produção de um anticorpo policlonal monoespecífico contra a proteína recombinante Nc-p43 e a avaliação de sua aplicabilidade como ferramenta no diagnóstico para neosporose. / Neospora caninum is a protozoan phylogenetically related to several coccidia with importance in veterinary medicine. This obligate intracellular parasite causes abortions in cattle and neoromuscular paralysis in dogs. Interaction ligand/receiver are required that allow the parasite explore your ability invasive, being the initial contact mediated for immunodominant surface proteins as Ncp43 and Nc-p29. The study of antigens and recombinant proteins of N. caninum have generated a serie of informations aiming the improvement of diagnosis and differentiation of this protozoan and other agents related to it, especially Toxoplasma gondii.In the present work was carried out a literature review focusing in the proteins involved in host-parasite interaction used as vaccine targets and application in diagnostic tests due to its specificity. Among the described proteins, antigens of surface Nc-p43 and Nc-p29 are widely studied due to their specifity and ability to induzer a protective immunity, and not reported cross reaction with other Apicomplexa parasite when used in trials diagnostics. Furthermore, the expression of specific proteins of N. caninum, Ncp43 and Nc-p29, in prokaryote system, are here described, followed by their evaluating concerning their antigenicity, using sera from animals naturally infected with N. caninum; and their specificity through reaction with sera from animals infected with T. gondii. The rNc-p43 protein was used for polyclonal antibody production in rabbit, purified and conjugated to peroxidase (HPR) and fluorescein isothiocyanate (FITC), in order to detect the recombinant and native Nc-p43, respectivelly. pAb and pAb/HRP were able to recognize rNc-p43, which was evaluated by Dot blot and ELISA assays, while pAb/FITC was able to mark the apical complex of tachyzoites. A blocking enzyme-linked immunosorbent assay (b-ELISA) was performed to evaluate the performance of pAb/HRP as diagnostic tool. The average percentage of inhibition for the positive sera pool and the negative sera pool of cattle neosporosis was significantly different (p <0.0001). These results suggest that pAb may bind to the same epitopes of Ncp43 and anti-N. caninum antibodies positive samples tested. b-ELISA using pAb/HRP is an interesting option for diagnostic tests that are available for neosporosis since fewer steps are involved in their implementation and their shape prevents cross-reactivity with anti-species-specific antibodies. In short, this work describes the cloning and expression of Nc-p43 and Nc-p29 proteins N. caninum in prokaryotic system, to produce a monnoespecific polyclonal antibody against the recombinant protein Nc-p43 and evaluation of its suitability as a tool in diagnosis of neosporosis.