As células pluripotentes (CPs), em teoria, são capazes de dar origem a todos os mais de 200 tipos de células do organismo. Na natureza, há três tipos de células pluripotentes: células-tronco embrionárias, células germinais embrionárias e células de carcinoma embrionário. As características das CPs têm permitido um importante avanço para a pesquisa básica e apontam uma grande aplicabilidade na medicina regenerativa. No núcleo das CPs existem fatores atuantes responsáveis pela manutenção da identidade pluripotente; dentre eles destacam-se OCT4, NANOG, SOX2, KLF4 e MYC. Muito já se sabe sobre os mecanismos que estes fatores atuam para promover a manutenção da pluripotência celular. Baseados nestes estudos foi possível gerar células de pluripotência induzida (iPSCs). Porém, os mecanismos moleculares que direcionam a indução da pluripotência ainda não estão muito bem esclarecidos. Alguns estudos revelaram que componentes chaves da via NFB estão envolvidos na regulação da pluripotência, bem como na diferenciação e destino celular das células-tronco. Neste estudo, analisamos a participação de componentes da via canônica (RelA e NFB1) e não-canônica (RelB e NFB2) de NFB nos processos de diferenciação e destino celular ou manutenção da pluripotência. Para isto usamos técnicas de PCR quantitativa em Tempo Real (qPCR) e Imunoprecipitação de Cromatina (ChIP) investigando os papéis das vias canônica e não-canônica de NFB na manutenção da pluripotência e diferenciação de CPs, em um modelo de indução de diferenciação celular mediado por ácido trans-retinóico (atRA) em células de carcinoma embrionário NTera-2. Foram avaliadas as ligações dos fatores de transcrição RelA e RelB nas regiões promotoras dos genes OCT4, SOX2, MYC, KLF4 e GFAP e a regulação transcricional associada. Nossos resultados identificaram que as células não tratadas com atRA apresentaram níveis baixos na expressão dos componentes da via canônica de NFB, RelA e NFB1, e GFAP e quando induzidas à diferenciação por atRA durante 4 dias esses níveis se elevaram. Uma situação oposta foi vista nos componentes da via não-canônica de NFB, RelB e NFB2, e na expressão dos fatores de pluripotência OCT4, NANOG, SOX2 e KLF4, que apresentaram níveis de expressão elevados nas células não tratadas com atRA e sofreram redução com a indução da diferenciação celular. O ensaio de ChIP revelou que RelA liga-se nas regiões de regulação dos genes OCT4, SOX2, KLF4, MYC e GFAP apenas quando a célula está em processo de diferenciação, enquanto RelB se apresentou ligado às mesmas regiões tanto nas células indiferenciadas quanto naquelas induzidas à diferenciação por 4 dias. Com estes dados sugerimos que a via canônica de NFB pode estar relacionada com o processo de diferenciação e destino celular através da regulação negativa executada por RelA e NFB1 nos genes responsáveis pela identidade pluripotente das células aqui estudadas enquanto a via não-canônica de NFB, representada pela ativação de RelB e NFKB2, pode participar na manutenção da pluripotência através da regulação positiva destes mesmos fatores. / Human pluripotent stem cells (hPSCs) are able to give rise to all the 200 cell types of the adult organism. In nature, there are three types of hPSCs: embryonic stem cells, germ line stem cells and embryonal carcinoma cells. hPSCs characteristics have allowed a major advance in basic research, and are thought to have great applicability in regenerative medicine. In the nucleus of hPSCs there are transcription factors responsible for the maintenance of their pluripotent identity. OCT4, NANOG, SOX2, KLF4 and MYC are considered the core pluripotency factors in hPSCs. A great deal of knowledge about the mechanisms that promote and maintain pluripotency has been generated. Based on these studies it was possible to generate induced pluripotent stem cells (iPSCs). However, the molecular mechanisms that drive the induction of pluripotency are not fully understood. Some studies have recently indicated that key components of the NFkB may be involved in regulating pluripotency as well as cell differentiation and cell fate. In this study we analyzed the involvement of components of the canonical (RelA and NFB1) and the non-canonical NFB pathways (RelB and NFB2) in the maintenance of pluripotency, differentiation and cell fate processes. The techniques of quantitative real-time PCR (qPCR) and chromatin immunoprecipitation (ChIP) were used to interrogate the roles of the canonical and non-canonical NFB pathways in maintenance of pluripotency and differentiation in a model of cell differentiation induced by all trans-retinoic acid (atRA) on embryonal carcinoma cells NTera-2. The transcription factors RelA and RelB occupancy in the promoter regions of OCT4, SOX2, KLF4, MYC and GFAP, and the transcriptional regulation associated were evaluated. Our results showed that undifferentiated cells exhibited low expression levels of canonical NFB pathway components, RelA and NFB1, while cells induced to differentiate for 4 days exhibited downregulated expression of these factors. In the other hand, the non-canonical NFB pathway components, RelB and NFB2, and the pluripotency factors OCT4, NANOG, SOX2 and KLF4 were expressed in higher levels in undifferentiated cells, and were downregulated upon the differentiation process. ChIP assay revealed that RelA binds to the regulatory regions of OCT4, SOX2, KLF4, MYC, and GFAP only when cells are induced to differentiate, while RelB was found bound to the same regions in both undifferentiated and differentiated cells. This data suggests that the canonical NFB pathway may be associated to differentiation and cell fate processes by downregulation of genes responsible for the pluripotent identity, and that the non-canonical NFB pathway may act in the maintenance of pluripotency through the upregulation of the same factors.

As células pluripotentes (CPs), em teoria, são capazes de dar origem a todos os mais de 200 tipos de células do organismo. Na natureza, há três tipos de células pluripotentes: células-tronco embrionárias, células germinais embrionárias e células de carcinoma embrionário. As características das CPs têm permitido um importante avanço para a pesquisa básica e apontam uma grande aplicabilidade na medicina regenerativa. No núcleo das CPs existem fatores atuantes responsáveis pela manutenção da identidade pluripotente; dentre eles destacam-se OCT4, NANOG, SOX2, KLF4 e MYC. Muito já se sabe sobre os mecanismos que estes fatores atuam para promover a manutenção da pluripotência celular. Baseados nestes estudos foi possível gerar células de pluripotência induzida (iPSCs). Porém, os mecanismos moleculares que direcionam a indução da pluripotência ainda não estão muito bem esclarecidos. Alguns estudos revelaram que componentes chaves da via NFB estão envolvidos na regulação da pluripotência, bem como na diferenciação e destino celular das células-tronco. Neste estudo, analisamos a participação de componentes da via canônica (RelA e NFB1) e não-canônica (RelB e NFB2) de NFB nos processos de diferenciação e destino celular ou manutenção da pluripotência. Para isto usamos técnicas de PCR quantitativa em Tempo Real (qPCR) e Imunoprecipitação de Cromatina (ChIP) investigando os papéis das vias canônica e não-canônica de NFB na manutenção da pluripotência e diferenciação de CPs, em um modelo de indução de diferenciação celular mediado por ácido trans-retinóico (atRA) em células de carcinoma embrionário NTera-2. Foram avaliadas as ligações dos fatores de transcrição RelA e RelB nas regiões promotoras dos genes OCT4, SOX2, MYC, KLF4 e GFAP e a regulação transcricional associada. Nossos resultados identificaram que as células não tratadas com atRA apresentaram níveis baixos na expressão dos componentes da via canônica de NFB, RelA e NFB1, e GFAP e quando induzidas à diferenciação por atRA durante 4 dias esses níveis se elevaram. Uma situação oposta foi vista nos componentes da via não-canônica de NFB, RelB e NFB2, e na expressão dos fatores de pluripotência OCT4, NANOG, SOX2 e KLF4, que apresentaram níveis de expressão elevados nas células não tratadas com atRA e sofreram redução com a indução da diferenciação celular. O ensaio de ChIP revelou que RelA liga-se nas regiões de regulação dos genes OCT4, SOX2, KLF4, MYC e GFAP apenas quando a célula está em processo de diferenciação, enquanto RelB se apresentou ligado às mesmas regiões tanto nas células indiferenciadas quanto naquelas induzidas à diferenciação por 4 dias. Com estes dados sugerimos que a via canônica de NFB pode estar relacionada com o processo de diferenciação e destino celular através da regulação negativa executada por RelA e NFB1 nos genes responsáveis pela identidade pluripotente das células aqui estudadas enquanto a via não-canônica de NFB, representada pela ativação de RelB e NFKB2, pode participar na manutenção da pluripotência através da regulação positiva destes mesmos fatores. / Human pluripotent stem cells (hPSCs) are able to give rise to all the 200 cell types of the adult organism. In nature, there are three types of hPSCs: embryonic stem cells, germ line stem cells and embryonal carcinoma cells. hPSCs characteristics have allowed a major advance in basic research, and are thought to have great applicability in regenerative medicine. In the nucleus of hPSCs there are transcription factors responsible for the maintenance of their pluripotent identity. OCT4, NANOG, SOX2, KLF4 and MYC are considered the core pluripotency factors in hPSCs. A great deal of knowledge about the mechanisms that promote and maintain pluripotency has been generated. Based on these studies it was possible to generate induced pluripotent stem cells (iPSCs). However, the molecular mechanisms that drive the induction of pluripotency are not fully understood. Some studies have recently indicated that key components of the NFkB may be involved in regulating pluripotency as well as cell differentiation and cell fate. In this study we analyzed the involvement of components of the canonical (RelA and NFB1) and the non-canonical NFB pathways (RelB and NFB2) in the maintenance of pluripotency, differentiation and cell fate processes. The techniques of quantitative real-time PCR (qPCR) and chromatin immunoprecipitation (ChIP) were used to interrogate the roles of the canonical and non-canonical NFB pathways in maintenance of pluripotency and differentiation in a model of cell differentiation induced by all trans-retinoic acid (atRA) on embryonal carcinoma cells NTera-2. The transcription factors RelA and RelB occupancy in the promoter regions of OCT4, SOX2, KLF4, MYC and GFAP, and the transcriptional regulation associated were evaluated. Our results showed that undifferentiated cells exhibited low expression levels of canonical NFB pathway components, RelA and NFB1, while cells induced to differentiate for 4 days exhibited downregulated expression of these factors. In the other hand, the non-canonical NFB pathway components, RelB and NFB2, and the pluripotency factors OCT4, NANOG, SOX2 and KLF4 were expressed in higher levels in undifferentiated cells, and were downregulated upon the differentiation process. ChIP assay revealed that RelA binds to the regulatory regions of OCT4, SOX2, KLF4, MYC, and GFAP only when cells are induced to differentiate, while RelB was found bound to the same regions in both undifferentiated and differentiated cells. This data suggests that the canonical NFB pathway may be associated to differentiation and cell fate processes by downregulation of genes responsible for the pluripotent identity, and that the non-canonical NFB pathway may act in the maintenance of pluripotency through the upregulation of the same factors.

This project is about the visual ways people represent the nonhuman world, and the struggles over its meaning. It is the story of how the Canadian government used the National Film Board of Canada (NFB) to manufacture and sustain a national identity that was defined by its encounters with nature, and how those definitions morphed over time. The NFB was established in 1939 by the federal government. It was to be the eyes and ears of Canada, a way for Canadians to experience the nation. As a cultural institution supported by the state, the NFB is fertile ground for an examination of state discourses about nature. In particular, I analyze NFB films as vehicles for the Canadian government’s long-running nation-building project. Between 1939 and 1974, NFB filmmakers aligned their representations of nature with the views of the government. They imagined nature as a unifying symbol of national identity and as an object to be surveyed, rationalized and exploited by government institutions. Utilitarian narratives about natural resources and wilderness management served other ideological motives too. Specifically, NFB films about nature in the postwar period privileged a high modern way of seeing the environment. This project also seeks to discern instances of ideological conflict between filmmakers and official “environmental” viewpoints, where government strategies are questioned, ridiculed or reformulated in the films themselves. Although the NFB is a product of state policy as well as an interpreter of it, it was also actively involved in producing grass-roots narratives about the environment. The NFB’s directive to “interpret Canada to Canadians” unwittingly created opportunities for independent filmmakers to share their own visions of nature that often diverged from the state. This project therefore investigates moments where filmmakers used the camera as an apparatus of reflection to challenge and subvert state modes of thinking. / Thesis / Doctor of Philosophy (PhD) / The National Film Board of Canada (NFB) was established and financed by the federal government to be the “eyes of Canada.” It is therefore a valuable site in which to examine, among other things, how the state defined the limits and uses of nature. While NFB discourses about the environment often mirrored state ideology, they also reflected alternative voices and perspectives. Filmmakers made documentaries within the NFB production system that challenged, questioned, or even ridiculed state ideology. In other words, nature was not only imagined as a national resource to be exploited and controlled through technology and science, it was also envisioned as something to be appreciated for its ecological diversity and its wildness.

Master thesis is dealing with issues and problems of packet queue management in high speed packet networks. Design implementation is made in VHDL hardware description language. In theoretical part of thesis are explained different types of mechanism used for providing quality of service in communication networks. Furthermore the brief description o VHDL, FPGA and framework Netcope Development Kit is a piece of theoretical part as well. The outcome of practical part contains a design, limiting packet queues based on Tocken Bucket mechanism. Design verification was made by simulations, synthesis and real implementation on smart NIC NFB-200G2QL. All kind of verificaion results are summerized in last three chapters.

Background:The family business is one of Sweden’s most common business forms, making up 90% of all firms and institutions. Also, it accounts for more than a third of GDP. So, it plays a vital role in the economy in Sweden. However, most of the family businesses are relatively small. Purpose:This paper aims to explore and understand the internal growth barriers to the small Swedish family business. Method:A qualitative method inspired by the inductive approach was conducted through semi-structured interviews with five owners of small family businesses in Sweden. The data were analyzed through three steps of general analytical procedure, which are (1) data reduction, (2) data displays, (3) conclusion and verification. Conclusion:This study concludes several internal growth barriers that affect the small Swedish family business: having only revenue goals, long-term growth goals, no written form of goals, family-oriented goals, unawareness of owner to change the firm’s structure during the growth, owner’s tendency to control all activities, owner prefers to keep the business smaller to keep control, the owner has more than one role and task, shortage of competencies and skills, unqualified successors and family members are welcome, lack of robots and Knowledge, owners have another job, different tasks, and roles, family business offers services of high quality that need time, selecting the oldest son to take over regardless of his competencies, employees’ attitude towards obeying a female owner, successors have no interest in taking over, no successors, rivalry among siblings and conflict of their interest, high arguments, different goals of family members from different generations, employ the first non-family member employee, employed more people, fear that non-family employees are less interested in FB or that more employees lead to loss of control, not able to employ more people and finally risk-avoiding behaviour. These barriers resulted in: hindering strategic changes in the needed time, innovation changes in the market obstructed, goals are forgotten, lack of competencies and skills, increases workload and challenges for the male manager, not responding to the market changes quickly, impossible to manage everything effectively, decrease control, not employing needed employees, responsibilities and roles on the owner increase, decrease integrity and harmony in the family, and finally, not developing the products, assisting more customers, and focusing on the growth. Consequently, lead to selling the firm and not keep it for a long time, slow growth, do no maximize potential growth, growth affected negatively, growth hindered, stay in the same size, avoid growth chances, or miss growth opportunities.

Voices and Silences: Exploring English and French Versions at the National Film Board of Canada, 1939-1974 Abstract The National Film Board of Canada (NFB) is unique as a state-sponsored filmmaking institution for its bilingualism: it has consistently produced and distributed films in English and French and made versions of some of those films in the opposite language. This doctoral thesis fills a gap in existing literature on the NFB and in translation studies by taking the versions as its object of study. The corpus is drawn from the vast body of audiovisual productions made by the NFB between 1939 and 1974, when voice-over was the preferred mode of audiovisual translation. Voice-over can refer to either the translated narration that replaces the original voice of narration-led documentaries, or to the audiovisual voice-over of documentaries built around interviews and spontaneous speech. Against the backdrop of asymmetrical language relations between English and French in Canada, this thesis offers a retelling of the NFB’s early history that emphasizes the intertwining threads of English and French production and identifies several approaches to version making. From 1939 to the mid-1950s, with English-language production dominant at the NFB and little original production in French, versions from English to French were a central element of film in Quebec. They bear witness to an interventionist approach to translating, whereby the original film is treated as raw material that can be shaped to appeal to local audiences. Subsequently, an increase in French original production, reflecting changing documentary aesthetics and growing nationalism in Quebec, led to a correspondingly higher number of French-to-English versions. These versions adopted a mediating approach by adding a narrator’s voice in English to originals that eschewed narration, bringing the innovative French films into conformity with the traditional model. The period from 1967 to 1974 is one of fragmentation and is characterized by a high level of non-translation, whether of the Challenge for Change/Société nouvelle films or those of Studio D. The few films that were versioned, however, showed great sensitivity to language. Non-translation of a different kind can be found in many Aboriginal films produced at the NFB. The NFB’s long-standing commitment to translation makes it a valuable site for studying audiovisual translation practices and changing language relations in Canada. Voix et silences : Une exploration des versions anglaises et françaises produites par l’Office national du film du Canada, 1939-1974 Résumé L’Office national du film du Canada (ONF), institution financée par l’État pour la création d’œuvres cinématographiques, se distingue par son bilinguisme, car il a toujours produit et distribué ses films en anglais ou en français tout en prenant soin de fournir une version dans la langue opposée. La thèse se penche sur ces versions et vient combler un vide dans les recherches sur l’ONF ainsi qu’en traductologie. Le corpus à l’étude provient d’une vaste production audiovisuelle réalisée entre 1939 et 1974, époque où le mode de traduction privilégié des documentaires était la voix hors-champ. Ce terme désigne autant la narration traduite qui remplace la voix originale que la voix en surimposition utilisée pour les entrevues et le discours spontané. La thèse relate les débuts de l’ONF dans le contexte des relations asymétriques entre l’anglais et le français au Canada et porte un regard neuf sur les liens entre les studios anglophones et francophones en plus de décrire certaines tendances dans la production de versions. De 1939 jusqu’au milieu des années 1950, lorsque l’anglais dominait, par rapport aux rares films produits originalement en français, les versions françaises traduites de l’anglais contribuèrent substantiellement à la cinématographie québécoise. Elles témoignent d’une stratégie de traduction interventionniste, par laquelle le film original est considéré comme une matière première que l’on peut manipuler afin d’accrocher le public local. Par suite d’un changement dans l’esthétique du documentaire et de la montée du nationalisme québécois, les originaux en langue française s’accrurent et entraînèrent une augmentation des versions du français à l’anglais. Celles-ci usèrent d’un mode de médiation qui consistait à rajouter une voix narrative anglophone aux films novateurs en français, du fait qu’ils avaient évacué la narration, soumettant ainsi les créations originales au modèle traditionnel. La période de 1967 à 1974 en est une de fragmentation se caractérisant par un nombre élevé de films non traduits, tels que ceux produits dans le cadre du programme Challenge for Change/Société nouvelle ou par le Studio D. Les quelques productions qui furent traduites, cependant, connurent des versions d’une grande sensibilité linguistique. Un phénomène de non-traduction, mais d’un autre ordre, s’observe par ailleurs dans certains films autochtones. Depuis longtemps, l’ONF s’est engagé à traduire ses productions; c’est ce qui en fait un site riche pour l’étude des pratiques de la traduction audiovisuelle et du changement des relations entre les langues au Canada.

This thesis describes the development of a networking device used to defend against (D)DoS attacks using P4 language. The main purpose was to design flexible device using P4 lan-guage based on already existing device, this would allow us to quickly react and respond to new more complex DDoS attacks. The design of the device dealt with the transfer of individual parts of the firmware into the P4 language. Subsequently, the entire device firmware was designed for hardware accelerators with FPGA technology. The firmware had been designed with respect to the limitations of current P4 language compilers. The device has been tested under laboratory conditions for functionality and performance. The device will be deployed in the network infrastructure of CESNET.